ライフサイエンスコーパス: phase separation

1 by the global environment (e.g. crowding or phase separation).

2 otides play a key role in modulating protein phase separation.

3 e the factors driving protein-polynucleotide phase separation.

4 ence of point-defect rich solid solution and phase separation.

5 ich in some cases form through liquid-liquid phase separation.

6 create lipid-free vesicle-like enclosures by phase separation.

7 parameter, such as temperature, that support phase separation.

8 emodeler Brg1 and FUS-assisted liquid-liquid phase separation.

9 covering proteins involved in liquid-liquid phase separation.

10 me asymmetry delays the onset of macroscopic phase separation.

11 t the charge properties of the PRD predicted phase separation.

12 binding protein in the case of liquid-liquid phase separation.

13 ng the molecular details of LLPS to modulate phase separation.

14 ol, PTM crosstalk, nucleosome structure, and phase separation.

15 PhaSepDB will facilitate the future study of phase separation.

16 ilaggrin assembles KGs through liquid-liquid phase separation.

17 air/water interface, possibly related to tau phase separation.

18 enomena, including swarming, clustering, and phase separation.

19 ckerboard charge disproportionation and nano phase separation.

20 and TBP) were similarly found to alter their phase separation.

21 eatest oligomer formation following extended phase separation.

22 wn the animals and triggers motility-induced phase separation.

23 ompaction states that are independent of HP1 phase separation.

24 rm in response to various stress stimuli via phase separation.

25 is a defining feature of proteins that drive phase separation.

26 enomenon in plants that might also arise via phase separation.

27 e, we show that Drosophila HLBs form through phase separation.

28 solute will stabilize or destabilize domain phase separation.

29 e heterochromatin formation by liquid-liquid phase separation.

30 how many proteins can undergo liquid-liquid phase separation.

31 e can serve as scaffold molecules that drive phase separation.

32 fied coronene could be separated via further phase separation.

33 affinities to high collective affinity upon phase separation.

34 structure in mammalian oocytes that forms by phase separation.

35 that assemble by intracellular liquid-liquid phase separation.

36 properties of proteins assembled via liquid phase separation.

37 tions between nucleosomes, thereby promoting phase separation.

38 liquid phases thereby regulating functional phase separation.

39 er droplets help understand the principle of phase separation.

40 nt low-complexity domain (LCD) promotes LEM2 phase separation.

41 rinciples and sequence features important to phase separation.

42 of biological condensates via liquid-liquid phase separation.

43 DP-43 CTD while GRN-5 mediated liquid-liquid phase separation.

44 tion species (PNS) that precede solid-liquid phase separation.

45 sate on the membrane surface can drive lipid phase separation.

46 ded high specificity for these selective gas-phase separations.

47 amellar vesicles capable of undergoing lipid phase separations.

48 es or condensates form through liquid-liquid phase separation(1-4), which is thought to underlie gene

49 The critical lengths required for phase separation (25 for poly-PR and 50 for poly-GA) are

50 Here we implicate liquid-liquid phase separation(3) as the underlying mechanism.

51 to the non-crosslinked emulsion that showed phase separation after two weeks of storage.

52 Phase separation also acts in cargo trafficking pathways

53 main from LAF-1, a model protein involved in phase separation and a key component of P granules.

54 0, and proteasome activities regulate TDP-43 phase separation and conversion into a gel or solid phas

55 chemical dimerization approach to manipulate phase separation and demonstrate how the material proper

56 -propelled rods destabilize motility-induced phase separation and facilitate orientational ordering,

57 dues can be a critical factor in controlling phase separation and highlights the unique role of NMR i

58 for the design of new ionomers with tunable phase separation and improved transport properties as we

59 top surface, changes the PBDB-TF/Y6 vertical phase separation and intermixing, and reduces the bottom

60 that the PrP interaction with NAs modulates phase separation and promotes PrP fibrillation in a NA s

61 nocrystallization involves an order-disorder phase separation and reconstruction, which is energetica

62 y-efficient, physical-chemical interactions, phase separation and self-assembly, are capable of gener

63 ng an unexpected mode of globally programmed phase separation and sequestration.

64 ear import but also, prevents their aberrant phase separation and stress granule recruitment in the c

65 levels below the critical concentration for phase separation and suppresses SG initiation.

66 A mutation in SynGAP-alpha1 that disrupts phase separation and synaptic targeting abolishes its ab

67 is close to liquid-disordered/liquid-ordered phase separation and that melatonin can induce phase sep

68 n how the FUS-RNA interactions contribute to phase separation and whether its phase behavior is affec

69 of cellular functions, including signaling, phase separation, and enzyme catalysis.

70 Macromolecule condensates, phase separation, and membraneless compartments have bec

71 interactions regulate nuclear localization, phase separation, and stress granule recruitment of CIRB

72 RNAs at the histone locus, which facilitates phase separation, and the nuclear concentration of the s

73 In many cases, phase separation appears to either underly or contribute

74 therefore effectiveness in altering membrane phase separation are strictly linked to the location of

75 has been previously appreciated, implicating phase separation as an evolutionarily ancient mechanism

76 or efforts have been devoted to identify the phase separation associated proteins and elucidate their

77 munity to easily browse, search and download phase separation associated proteins.

78 blication reference and sequence features of phase separation associated proteins.

79 Phase separation at specific sites of the cell periphery

80 Finally, we propose a metaphor for rapid phase separation based on cloud formation, reasoning tha

81 We find a strong dependence of the phase-separation behavior on both DPR length and concent

82 pleted cells, potentially as a result of the phase separation between the two chromatin segments with

83 relative average phase purity, or TEM-imaged phase separation but rather facilitates Y6 migration to

84 neless organelles form through liquid-liquid phase separation, but how their size is controlled and w

85 We directly test the in vivo function of phase separation by using mutant SYD-2 and ELKS-1 protei

86 nsic contributions of disordered proteins to phase separation can be discerned by computing or measur

87 Reentrant liquid-liquid phase separation can occur when the condensation of one

88 Our study demonstrates how regulated phase separation can simultaneously enhance reaction eff

89 reditary synpolydactyly in humans, alter its phase separation capacity and its capacity to co-condens

90 developed a five-compartment electrodialysis/phase separation cell (ED/PS).

91 Yet, how phase separation contributes to the physiological functi

92 n mutation in patient cells, suggesting that phase separation could play an important role in DPR tox

93 ity (J(sc)) results from the smaller polymer phase-separation domain sizes as evidenced by PL quenchi

94 es the photovoltaic performance as the donor phase-separation domain sizes increases.

95 rthermore, we observe that the induced lipid phase separation drives localization of the SOS substrat

96 Phase separation drives numerous cellular processes, ran

97 so known as liprin-alpha) and ELKS-1 undergo phase separation during an early stage of synapse develo

98 idermal structure and function are driven by phase-separation dynamics.

99 Condensate formation by phase separation emerges as a new principle to explain t

100 Dynamic phase separation enables rapid mobilization of this prot

101 ample, solar cell degradation mechanisms via phase separation, especially for more complex high-perfo

102 A growing body of evidence suggests that phase separation events likely play a significant and mu

103 butions of tyrosine and arginine residues to phase separation experimentally through mutagenesis stud

104 ve the key PTM connectivity, which condensed-phase separations failed to achieve.

105 o-step process involving first liquid-liquid phase separation followed by polymer microphase separati

106 ular condensation, as well as for harnessing phase separation for applications in bioinspired materia

107 The dynamics of phase separation governed terminal differentiation and w

108 Biological liquid-liquid phase separation has gained considerable attention in re

109 Lipid miscibility phase separation has long been considered to be a centra

110 cal tests of this model in living cells, and phase separation has not yet been directly linked with d

111 Biomolecular droplets formed through phase separation have a tendency to fuse.

112 In recent years, condensates formed by phase separation have emerged as a new principle governi

113 he design principles underlying biomolecular phase separation have the potential to drive the develop

114 ingly, this rapid intracellular hyperosmotic phase separation (HOPS) correlates with the degree of ce

115 bsorption, enhanced crystallinity, prominent phase separation, improved mobility, and decreased charg

116 inhibitors and improve our understanding of phase separation in cells and membraneless organelles.

117 Lipid phase separation in cellular membranes is thought to pla

118 dilute solutions and the driving forces for phase separation in concentrated solutions.

119 To address the possible role of phase separation in DPR toxicity, a one-bead-per-amino-a

120 h as endocytosis and physical phenomena like phase separation in lipid bilayers.

121 cular dynamics simulations have investigated phase separation in model membranes at the coarse-graine

122 is Review, we examine the emerging roles for phase separation in plants.

123 d discussing some context-dependent roles of phase separation in regulating biochemical reactions.

124 At 5 mol% melatonin, we observed phase separation in samples with POPC-d(31), but not wit

125 s particularly focused on the role played by phase separation in the formation of molecule aggregates

126 Appreciation for the role of liquid-liquid phase separation in the functional organization of cellu

127 N" diblock copolymers that undergo nanoscale phase separation in the solid state to produce sub-10 nm

128 xperiments, we demonstrate that DDXs promote phase separation in their ATP-bound form, whereas ATP hy

129 ase separation and that melatonin can induce phase separation in these ternary mixtures by preferenti

130 cidating the molecular basis of biomolecular phase separation in various disease, stress response, an

131 her of these positions dramatically enhances phase separation in vitro and decreases fluidity of phas

132 TIAR-2 undergoes liquid-liquid phase separation in vitro and forms granules with liquid

133 letion of this region significantly disrupts phase separation in vitro and in vivo.

134 TDP-43 CTD aggregation and/or liquid-liquid phase separation in vitro GRN-3 promoted insoluble aggre

135 of proteins as a function of pH and protein phase separation in yeast cells for pH values close to t

136 mediate transcriptional changes possibly via phase separation, in a manner likely dependent on the fu

137 Thus, SAM-induced phase separation, in the context of Ph, can mediate larg

138 in a manner that is at least consistent with phase separation, including nucleoli, stress granules, C

139 We also found the extent of GQ-triggered-phase-separation increases on exposure to conditions whi

140 ges as attempts to make them often result in phase separation into binary compounds.

141 ct domains within Ede1 bind Atg8 and mediate phase separation into condensates.

142 Liquid phase separation into two or more coexisting phases has

143 Phase separation is a cooperative process, the kinetics

144 This phase separation is affected by pH via a set of chemical

145 Phase separation is an emerging paradigm for understandi

146 Phase separation is an important mechanism that mediates

147 rotein sequence features responsible for IDP phase separation is critical for understanding physiolog

148 ange their binding and subsequent control of phase separation is critical to designing better pathoge

149 Phase separation is crucial for selective surfactant for

150 ions; thus, a proteome-wide understanding of phase separation is currently lacking.

151 Liquid-liquid phase separation is emerging as the universal mechanism

152 N protein phase separation is induced by addition of non-specific

153 f membrane-less organelles via liquid-liquid phase separation is one way cells meet the biological re

154 concentrated within the condensates, whereas phase separation is overall regulated by the stoichiomet

155 iologically-motivated modeling revealed that phase separation is suppressed by a "magic-number effect

156 Phase separation is thought to underlie spatial and temp

157 ition, our model predicts that the region of phase separation is typically broader at the isoelectric

158 We suggest that the CPC combines phase separation, kinase and histone code-reading activi

159 eir plurality through the unifying canvas of phase separation kinetics.

160 in 1alpha (HP1alpha) undergoes liquid-liquid phase separation (LLPS) and forms liquid droplets and ge

161 we asked whether PrP undergoes liquid-liquid phase separation (LLPS) and if this process is modulated

162 ties, the CPC also can undergo liquid-liquid phase separation (LLPS) and proposed that the inner cent

163 -CoV-2) condenses with RNA via liquid-liquid phase separation (LLPS) and that N protein can be recrui

164 show that SGs assemble through liquid-liquid phase separation (LLPS) arising from interactions distri

165 nase (PKA), RIalpha, undergoes liquid-liquid phase separation (LLPS) as a function of cAMP signaling

166 Here, we identify liquid-liquid phase separation (LLPS) as a mechanism for organizing cl

167 e organisms, for instance, use liquid-liquid phase separation (LLPS) as the precursor phase to form v

168 Here, we uncover a common liquid-liquid phase separation (LLPS) behavior shared by these disease

169 tau has been shown to undergo liquid liquid phase separation (LLPS) both in vivo and in vitro.

170 wetting layer when approaching liquid-liquid phase separation (LLPS) by changing protein concentratio

171 Liquid-liquid phase separation (LLPS) compartmentalizes transcriptiona

172 0/Na(2)SO(4)/Water system near Liquid-Liquid Phase Separation (LLPS) conditions by both sitting-drop

173 condensate, whose formation by liquid-liquid phase separation (LLPS) facilitates the initial steps of

174 -like domains (PLDs) can drive liquid-liquid phase separation (LLPS) in cells.

175 ion (IDR) that facilitates its liquid-liquid phase separation (LLPS) in the nucleolus.

176 rease of NEAT1 promotes TDP-43 liquid-liquid phase separation (LLPS) in vitro.

177 n interacting sites, undergoes liquid-liquid phase separation (LLPS) in vitro.

178 Liquid-liquid phase separation (LLPS) is involved in the formation of

179 Liquid-liquid phase separation (LLPS) is one proposed mechanism for me

180 The idea that liquid-liquid phase separation (LLPS) may be a general mechanism by wh

181 Liquid-liquid phase separation (LLPS) mediates formation of membranele

182 Liquid-liquid phase separation (LLPS) occurs following amorphous solid

183 Proteinaceous liquid-liquid phase separation (LLPS) occurs when a polypeptide coales

184 Liquid-liquid phase separation (LLPS) of proteins and nucleic acids is

185 Liquid-liquid phase separation (LLPS) of proteins that leads to format

186 Liquid-liquid phase separation (LLPS) of proteins underlies the format

187 cular condensates underlain by liquid-liquid phase separation (LLPS) of proteins, we conducted multip

188 Cellular liquid-liquid phase separation (LLPS) plays a key role in the dynamics

189 study, Yasuda et al. show how liquid-liquid phase separation (LLPS) under hyperosmotic stress condit

190 It's widely appreciated that liquid-liquid phase separation (LLPS) underlies the formation of membr

191 protein (N-protein) undergoes liquid-liquid phase separation (LLPS) with viral RNA.

192 or condensates, that form via liquid-liquid phase separation (LLPS)(1,2).

193 Biomolecules can undergo liquid-liquid phase separation (LLPS), forming dense droplets that are

194 owding agents, tau can undergo liquid-liquid phase separation (LLPS), forming highly dynamic liquid d

195 rts that tau readily undergoes liquid-liquid phase separation (LLPS), here we explored the relationsh

196 eless organelles assembled via liquid-liquid phase separation (LLPS), known as condensates, also faci

197 ough they are likely formed by liquid-liquid phase separation (LLPS), they have a differential sensit

198 n IAPP undergoes AWI-catalyzed liquid-liquid phase separation (LLPS), which initiates hydrogelation a

199 proteins (RBPs), which undergo liquid-liquid phase separation (LLPS).

200 ng proteins and are formed via liquid-liquid phase separation (LLPS).

201 norganic system that undergoes liquid-liquid phase separation (LLPS).

202 omolecular condensates through liquid-liquid phase separation (LLPS).

203 u protein in vitro can undergo liquid-liquid phase separation (LLPS); however, observations of this p

204 opathies, was found to undergo liquid-liquid phase separation making it one of several proteins assoc

205 nuclear membrane proteins(7,8), suggest that phase separation may contribute to other critical envelo

206 ecific transcription factors also engage the phase-separation mechanism for efficient and specific tr

207 eered platform for creating puncta with new, phase-separation-mediated control of biological function

208 from proteins and RNAs through liquid-liquid phase separation, membraneless organelles (MLOs) have em

209 cells and examine the various ways in which phase separation might contribute to the development of

210 The defects are rescued by introducing a phase-separation motif from an unrelated protein.

211 y concentrated molecules akin to biophysical phase separation observable in vitro.

212 (60 degrees C) resulted in minimal degree of phase separation observed.

213 The phase separation occurring in a system of mutually inter

214 Specifically, we analyze the liquid-liquid phase separation of an in vitro model of cellular membra

215 the phase diagram and are formed through the phase separation of anisotropic protein-RNA complexes.

216 less organelles resulting from liquid-liquid phase separation of biopolymers into intracellular conde

217 r results provide critical insights into the phase separation of cellular membranes and, more general

218 vides a scaffold that supports liquid-liquid phase separation of chromatin binding proteins.

219 pmental biology and to test concepts such as phase separation of chromatin.

220 oters drives RNA synthesis, which stimulates phase separation of DDR factors in the shape of foci.

221 Here, we exploit the aqueous two-phase separation of dextran-in-PEG emulsion micro-drople

222 In pollen grains, a phase separation of extracellular material into a patter

223 uidic device that triggered liquid-to-liquid phase separation of FG-Nups, which yielded droplets that

224 g this sequence completely prevents in vitro phase separation of full-length hnRNPA2 and aggregation

225 ce stimulates granule formation by enhancing phase separation of hnRNPA2 has not yet been studied.

226 Phase separation of intrinsically disordered proteins (I

227 Phase separation of intrinsically disordered proteins (I

228 e, a process implicated in the liquid-liquid phase separation of intrinsically disordered proteins.

229 lation of the dynamic of the Taylor cone and phase separation of its ejected droplets enable the gene

230 transition results from electrically driven phase separation of Li(4) Ti(5) O(12) and Li(7) Ti(5) O(

231 able surface patterns were created through a phase separation of liquid crystal oligomers (LCOs) drop

232 l model to study the effects of pH on liquid phase separation of macromolecules.

233 Phase separation of membrane-associated proteins partici

234 Liquid-liquid phase separation of multivalent intrinsically disordered

235 hich are membraneless compartments formed by phase separation of polyelectrolyte solutions.

236 This model could account for both in vitro phase separation of proteins as a function of pH and pro

237 Phase separation of substrates and effectors is proposed

238 These findings suggest that phase separation of tau may facilitate the formation of

239 Liquid-liquid phase separation of tau protein has been implicated in n

240 titution with the YAP CC domain prevents the phase separation of TAZ and its ability to induce the ex

241 ndocytosis (CME) proteins facilitated by the phase separation of the CME protein, Ede1, which acts as

242 the acidity for adsorption of antibiotic and phase separation of the copolymers within the polymerizi

243 We find that coacervates formed by phase separation of the shorter polyions more effectivel

244 We recently developed orthogonal organic phase separation (OOPS): a quick, efficient and reproduc

245 might result from a process of liquid-liquid phase separation orchestrated by the epigenetic marking

246 e examine in vitro hnRNPA2 granule component phase separation, partitioning specificity, assembly/dis

247 Liquid-liquid phase separation plays an important role in cellular org

248 PA2 tyrosine phosphorylation reduces hnRNPA2 phase separation, prevents partitioning of hnRNPF and ch

249 der applicability of the results because the phase separation process is dictated by the inherent asy

250 presence of regulatory sites can promote the phase-separation process.

251 Extended phase separation promoted a time-dependent adoption of t

252 igned sequences with significantly increased phase separation propensity by shuffling the wild-type s

253 ighted the diverse function of RNA in tuning phase-separation propensity up or down, altering viscoel

254 he relations between amino acid sequence and phase-separation propensity.

255 del is used to study the single-molecule and phase-separation properties of the DPRs.

256 ous posttranslational modifications and its "phase-separation" properties.

257 ts that the spatial proximity resulting from phase separation reduces the requirement for motif speci

258 ining region of this construct for promoting phase separation relative to other Arg-rich stretches of

259 es that occurs during PISA leads to enhanced phase separation relative to that achieved using solutio

260 hly concentrated and dynamic condensates via phase separation, reminiscent of stargazin/PSD-95-mediat

261 ropelled hard discs undergo motility-induced phase separation, self-propelled rods exhibit a variety

262 e saturation concentration for liquid-liquid phase separation, so they can compete subunits away from

263 iency of cross-links, here, we present a gas-phase separation strategy using high-field asymmetric wa

264 ontaneously formed by macromolecules through phase separation, suggest new possibilities for how enzy

265 's disease, were found to be highly prone to phase separation, suggesting that there might be a stron

266 ons to viscoadaptation affect solubility and phase separation, suggesting that viscoadaptation may ha

267 all, we provide an account of how biological phase separation supports the highly intertwined relatio

268 rasonication technique and investigate their phase separation, surface oxidation, and nucleation.

269 cal studies reveal two features of reentrant phase separation that are likely important to functional

270 After phase separation, the bilayer organizes in multiple lipi

271 to understand the principles of heterotypic phase separation, the demixing of multiple proteins and

272 , we demonstrate a strong connection between phase separation, the process underlying the formation o

273 homogeneous swimmer suspension that mimics a phase separation; the process is mediated by intercellul

274 We propose that plants make use of phase separation to a much greater extent than has been

275 transcriptional components drives localized phase separation to amplify gene expression.

276 ms nuclear condensates through liquid-liquid phase separation to compartmentalize its DNA-binding cof

277 omains, and that can engage in liquid-liquid phase separation to form subnuclear bodies, as well as b

278 review summarizes recent work linking liquid phase separation to neurodegeneration, highlighting a pa

279 multivalency required to facilitate protein phase separation to regulate protein function.

280 ses by biomolecular condensates that form by phase separation to suggest that the destruction complex

281 chromatin and transcription factors through phase separation to sustain gene transcription in chroma

282 These results link phase separation to tumorigenesis and uncover an importa

283 e that the physical process of liquid-liquid phase separation, together with surface effects, is suff

284 es between lipid domains and its relation to phase separation, ultimately providing a platform for id

285 raction of the proteome is poised to undergo phase separation under physiological conditions.

286 (KTP)-rich phase generated by liquid-liquid phase separation was evaluated.

287 Phase separation was higher in PBAT/PLA films due to les

288 th DPPC-d(62), whereas at 10 mol% melatonin, phase separation was observed in both samples with eithe

289 ur knowledge of sequence determinants of IDP phase separation, we characterized variants of the intri

290 In the regime of dense clusters akin to phase-separation, we observe co-existing assemblies, in

291 gns of related physicochemical process (i.e. phase separation) were also observed.

292 s, which have previously been linked to PrLD phase separation, were dispensable for this recruitment.

293 g conditions, degradation that occurs due to phase separation when mixed halides are employed.

294 he structure of the GM headgroup affects the phase separation, whereas the nature of the tail determi

295 This phase separation, which creates a distortion in the dire

296 prone to undergoing concentration-dependent phase separation, which on aging is rationalized in a de

297 ther signatures characteristic of electronic phase separation, which we attribute to emergence of str

298 SynGAP-alpha1, which undergoes liquid-liquid phase separation with PSD-95, is highly enriched in syna

299 Along this trajectory, function evolved from phase separation with RNA (coacervates) to avid and spec

300 These condensed states are formed by phase separation, yet little is known about how material