ライフサイエンスコーパス: phorbol 12,13-dibutyrate

1 ing affinity (Kd = 2890 +/- 240 nm for [(3)H]phorbol 12,13-dibutyrate.

2 ensin II and the protein kinase C activator, phorbol 12,13-dibutyrate.

3 n PKCdelta have similar binding affinity for phorbol 12,13-dibutyrate.

4 igher potency than oleoyl-acetyl-glycerol or phorbol 12,13-dibutyrate.

5 with the increase of PKD activity induced by phorbol 12,13-dibutyrate.

6 ther enhanced by treatment of the cells with phorbol 12,13-dibutyrate.

7 protein kinase C (PKC) down-regulation with phorbol 12,13-dibutyrate (1 microM for 24 h) but not by

8 umol/L), and the protein kinase C activator, phorbol 12-13 dibutyrate (1 mumol/L), increased Cl- infl

9 nd inhibited Icat2 whereas the PKC activator phorbol-12,13-dibutyrate (1 microM) reduced Ang II-induc

10 well as by protein kinase C (PKC) activators phorbol 12,13-dibutyrate (10 micro;M) and phorbol 12-myr

11 Phorbol 12,13-dibutyrate (10 microM) and phorbol 12-myri

12 tion of (-)-indolactam V (0.03-30 microM) or phorbol-12,13-dibutyrate (10 microM) reversibly reduced

13 ter 12-myristate 13-acetate (PMA, 100 nM) or phorbol-12, 13-dibutyrate (100 nM) reduced taurine uptak

14 The phorbol ester [(3)H]phorbol 12, 13-dibutyrate ([(3)H]PDBu) bound to this C1

15 und phorbol esters, but the binding of [(3)H]phorbol 12,13-dibutyrate ([(3)H]PDBu) by the deltaC1a do

16 Binding assays using [(3)H]phorbol 12,13-dibutyrate ([(3)H]PDBu) revealed that inte

17 ong-term consequences of acute stress on [3H]phorbol 12,13-dibutyrate ([3H]PDBu) binding, a marker fo

18 ed by quantitative autoradiography using [3H]phorbol-12,13-dibutyrate ([3H]PDBu) binding.

19 production or contraction induced by Ca(2+), phorbol 12,13-dibutyrate (a protein kinase C activator),

20 Exposure to 100 nm phorbol 12,13-dibutyrate, a direct activator of PKC, eli

21 25% of their initial level by treatment with phorbol 12,13-dibutyrate also abolished the TPO-induced

22 Phorbol-12, 13-dibutyrate, an activator of protein kinas

23 ansfected with human PS1, we have found that phorbol 12, 13-dibutyrate and forskolin increase the sta

24 Phorbol 12,13-dibutyrate and 1-oleyl-2-acetyl-glycerol c

25 bated in the presence of phosphatidylserine, phorbol 12,13-dibutyrate and ATP, intact PKD slowly auto

26 ained by gastric biopsy were stimulated with phorbol 12,13-dibutyrate and ionomycin in the presence o

27 The more hydrophilic compounds (phorbol 12,13-dibutyrate and phorbol 12,13-dihexanoate)

28 st to palytoxin, the TPA-type tumor promoter phorbol 12,13-dibutyrate and the non-TPA-type tumor prom

29 Prolonged exposure to phorbol-12,13-dibutyrate and acetylcholine yielded more

30 now report that protein kinase C activators, phorbol 12,13-dibutyrate, and phorbol 12-myristate 13-ac

31 Interestingly, phorbol 12,13-dibutyrate, another phorbol ester, and IL-

32 Addition of phorbol 12,13-dibutyrate at lower concentrations (3 and

33 that was active in the process of inhibiting phorbol 12,13-dibutyrate binding to partially purified p

34 elerythrine and angoline did not inhibit [3H]phorbol 12,13-dibutyrate binding to the regulatory domai

35 quirement for anionic phospholipid for [(3)H]phorbol 12,13-dibutyrate binding was determined; it decr

36 erved after treatment with the PKC activator phorbol-12,13-dibutyrate but not after treatment with an

37 tein kinase C isoforms (by pretreatment with phorbol 12,13-dibutyrate) but not by pretreatment with G

38 ic for PKC as the PMA effect was mimicked by phorbol 12,13-dibutyrate, but not by 4alpha-phorbol 12,1

39 A similar effect was observed with phorbol 12,13-dibutyrate, but not with the biologically

40 Furthermore, activation of PKC by phorbol-12,13-dibutyrate did not produce long-term chang

41 In addition, acute application of phorbol 12,13-dibutyrate enhanced peak IBa density in co

42 n of PKCdelta abolished its high potency for phorbol 12,13-dibutyrate in vitro, with only marginal re

43 tivity stimulated by a phorbol ester (4 beta-phorbol 12,13-dibutyrate) in endothelial cells was inhib

44 Phorbol-12-myristate-13-acetate and phorbol-12, 13-dibutyrate increased PKC activity but fai

45 y elevated [Ca++] (ca. 3 x 10(-7) M), or the phorbol-12,13-dibutyrate-induced inhibition of Ca++-acti

46 hibited LFA-1/ICAM-1-dependent adhesion in a phorbol-12,13-dibutyrate-induced model of tonsil T cell

47 ma cell line PANC-1 with biologically active phorbol-12,13-dibutyrate led to striking activation of p

48 PKC inhibitor GF 109203X (50 +/- 4%) and by phorbol-12, 13-dibutyrate-mediated down-regulation of PK

49 The PKC activator phorbol-12,13-dibutyrate mimicked the effect of ethanol,

50 response to protein kinase C activation with phorbol 12,13-dibutyrate or 1-oleyl-2-acetyl-glycerol.

51 Galpha(s)-stimulated AC7 activity by either phorbol 12,13-dibutyrate or ethanol, in HEL cells endoge

52 phosphatase activity during stimulation with phorbol-12,13-dibutyrate or acetylcholine.

53 tein kinase C (PKC) activity with 0.5 microM phorbol-12,13-dibutyrate or briefly incubating cells wit

54 lasma membrane confines after stimulation by phorbol-12,13-dibutyrate or forskolin, respectively.

55 7 and HL-60 myeloid leukemia cells with TPA, phorbol-12,13-dibutyrate, or bryostatin 1 was associated

56 In contrast to phorbol 12,13-dibutyrate, palytoxin does not activate th

57 fied human PKD and either wild-type PKD from phorbol 12, 13-dibutyrate (PDB)-stimulated cells or unst

58 strongly activated all of these kinases, and phorbol 12,13-dibutyrate (PDB), which strongly activated

59 activation of protein kinase C isoforms with phorbol-12,13-dibutyrate (PDB) also promoted striking ph

60 was identified by titrating this domain with phorbol-12,13-dibutyrate (PDB) in the presence of organi

61 SCLC cell lines H 69, H 345, and H 510 with phorbol-12,13-dibutyrate (PDB) led to a rapid and striki

62 beta-phorbol 12-myristate, 13-acetate (PMA), phorbol 12, 13 dibutyrate (PDBu) and 12-deoxyphorbol 13-

63 e effect of ATPgammaS while a PKC activator, phorbol 12, 13-dibutyrate (PDBu) activated a current wit

64 Maximal activation of PKC by 100 nM phorbol 12, 13-dibutyrate (PdBu) almost completely inhib

65 Application of the PKC activator phorbol 12, 13-dibutyrate (PDBu) and chelerythrine, resp

66 owed previously that treatment with 1 microM phorbol 12, 13-dibutyrate (PDBU) for 24 h completely blo

67 rs phorbol 12-myristate 14-acetate (PMA) and phorbol 12, 13-dibutyrate (PDBu) inhibited KIR2.3 curren

68 -O-tetradecanoylphorbol-13-acetate (TPA) and phorbol 12, 13-dibutyrate (PDBu) inhibited secretin-evok

69 Reverse dialysis of a PKC activator, phorbol 12,13-dibutyrate (PDBu) (0.5 microM), through th

70 A phorbol ester phorbol 12,13-dibutyrate (PDBu) (a diacylglycerol analog

71 The PKC activator phorbol 12,13-dibutyrate (PDBu) alone increased mEPSC ev

72 id ligand for some PH domains, reconstitutes phorbol 12,13-dibutyrate (PDBu) binding to PKD similarly

73 [(3)H]Phorbol 12,13-dibutyrate (PDBu) binding was carried out

74 We also show that PKC activation with phorbol 12,13-dibutyrate (PDBu) causes a 4-fold slowing

75 aline, 1-oleoyl-acetyl-sn-glycerol (OAG) and phorbol 12,13-dibutyrate (PDBu) evoked single channel cu

76 ms of their ability to displace bound [3H-20]phorbol 12,13-dibutyrate (PDBU) from the single isozyme

77 (o) of SOCs stimulated by the phorbol ester, phorbol 12,13-dibutyrate (PDBu) in inside-out patches an

78 Preincubation of sickle erythrocytes with phorbol 12,13-dibutyrate (PDBu) increased adherence of s

79 By activating PKC with the phorbol ester phorbol 12,13-dibutyrate (PDBu) or a natural stimulant,

80 ctivation of PKC by pretreatment with 100 nM phorbol 12,13-dibutyrate (PDBu) significantly inhibited

81 eir ability to inhibit the binding of [3H-20]phorbol 12,13-dibutyrate (PDBU) to PK-C alpha, the enant

82 This gene is highly stimulated by phorbol 12,13-dibutyrate (PDBu) via three pathways: (i)

83 lated by CPA, BAPTA-AM and the phorbol ester phorbol 12,13-dibutyrate (PDBu) were reduced by anti-PIP

84 [3H]Forskolin and [3H]phorbol 12,13-dibutyrate (PDBu) were used to label adeny

85 In addition, the PKC activator phorbol 12,13-dibutyrate (PDBu), a PKC catalytic subunit

86 erythrine and activated by the phorbol ester phorbol 12,13-dibutyrate (PDBu), the diacylglycerol anal

87 Submicromolar phorbol 12,13-dibutyrate (PDBu), which stimulates PKC, a

88 e, KCl-dependent membrane depolarization and phorbol 12,13-dibutyrate (PDBu).

89 sin or the biologically active phorbol ester phorbol 12,13-dibutyrate (PDBu).

90 y up to eightfold, as did the phorbol ester, phorbol 12,13-dibutyrate (PDBu).

91 Phorbol 12,13-dibutyrate (PDBu, 0.1 micromol/L) increase

92 M) activated Icat, whereas the phorbol ester phorbol 12,13-dibutyrate (PDBu, 0.1-5 microM) failed to

93 Activation of protein kinase C by phorbol 12,13-dibutyrate (PDBu, 100 nM) inhibited the in

94 on of endogenous protein kinase C (PKC) with phorbol 12,13-dibutyrate (PDBu, 100 nmol/L) evoked a Cl-

95 , completely abolished Ca2+ sensitization by phorbol 12,13-dibutyrate (PDBu; 1 microM).

96 decanoylphorbol 13-acetate (TPA; 162 nM) and phorbol 12,13-dibutyrate (PDBu; 100-200 nM) each increas

97 Activators of PKC, such as phorbol-12, 13-dibutyrate (PDBu) (1.0 microM), indolacta

98 After activation of PKC by 100 nM phorbol-12, 13-dibutyrate (PDBu), [3H]MK-801 binding was

99 cidil, the protein kinase C activator 4 beta-phorbol-12, 13-dibutyrate (PDBu), or standard potassium-

100 a dose 10-fold higher than with the standard phorbol-12, 13-dibutyrate (PDBU).

101 luding a diacylglycerol/phorbol ester [4beta-phorbol-12, 13-dibutyrate (PDBu)] binding C1 domain.

102 ment of guinea pig ventricular myocytes with phorbol-12,13-dibutyrate (PDBu) caused a significant dec

103 stimulation protocol with the phorbol ester phorbol-12,13-dibutyrate (PDBu) does not induce a high a

104 rs phorbol-12-myristate-13-acetate (PMA) and phorbol-12,13-dibutyrate (PDBu) increased the amplitude

105 dye FM 1-43, we have examined the effects of phorbol-12,13-dibutyrate (PDBu) on presynaptic vesicle t

106 etyl-sn-glycerol (OAG) or the phorbol ester, phorbol-12,13-dibutyrate (PDBu) was also markedly inhibi

107 ed the binding models of phorbol-13-acetate, phorbol-12,13-dibutyrate (PDBu), indolactam V (ILV), ing

108 ures after stimulation with a phorbol ester, phorbol-12,13-dibutyrate (PDBu).

109 pidermal growth factor (ErbB1 activation) or phorbol 12,13-dibutyrate (PKC activation).

110 hard plot analysis using the radioligand [3H]phorbol 12,13-dibutyrate revealed a dissociation constan

111 10R/L20R) compared to the wild-type, whereas phorbol 12,13-dibutyrate showed a 6000-fold loss of affi

112 ity (Ki= 1.78 nm) only for deltaC1b, whereas phorbol 12,13-dibutyrate showed affinities within 10-fol

113 Further, phorbol 12,13-dibutyrate significantly increased the amo

114 Upon phorbol 12,13-dibutyrate stimulation, green fluorescent

115 Relative to phorbol 12,13-dibutyrate, they showed 15- and 6-fold sel

116 Inhibition of the binding of [3H]phorbol-12,13-dibutyrate to PK-C alpha showed that only

117 rolonged treatment of native thymocytes with phorbol 12,13-dibutyrate together with the calcium ionop

118 e potentiation of AC7 activity by ethanol or phorbol 12,13-dibutyrate was found to be reduced by the

119 Similar results were obtained when phorbol-12, 13-dibutyrate was used instead of TPA.

120 leoyl-2-acetyl-sn-glycerol increased Po, but phorbol 12,13-dibutyrate, which stimulates protein kinas

121 The MRCK C1 domains bind [20-(3)H]phorbol 12,13-dibutyrate with K(d) values of 10 and 17 n

122 induced by high concentrations of glucose or phorbol 12,13-dibutyrate without altering values obtaine