ライフサイエンスコーパス: phosphohistidine

1 t, 13.5 ppm) to the phosphoryl moiety of the phosphohistidine.

2 hoamino acid analysis showed the presence of phosphohistidine.

3 analogy to the NMR properties of the known 3-phosphohistidine.

4 ino acid found after alkaline hydrolysis was phosphohistidine.

5 nstants consistent with published values for phosphohistidine.

6 mblance to other enzymes known to contain N3-phosphohistidine.

7 rylated residue co-migrated with authentic 1-phosphohistidine.

8 e nitrogens can be phosphorylated, forming 1-phosphohistidine (1-pHis) or 3-phosphohistidine (3-pHis)

9 ed, forming 1-phosphohistidine (1-pHis) or 3-phosphohistidine (3-pHis).

10 d ESCC cells using antibodies specific for 3-phosphohistidine and mass spectrometry.

11 oteins, and the heat and acid sensitivity of phosphohistidine and phosphoaspartate complicates substr

12 osphoenzyme intermediate was consistent with phosphohistidine and the only radioactive amino acid fou

13 nd phosphotyrosine-containing proteins and 3-phosphohistidine- and phospholysine-containing amino aci

14 Upon formation of the phosphohistidine at His-258, the 13C and 1H resonances o

15 the phosphoaccepting histidine and from the phosphohistidine back to ADP seem to be essentially equa

16 duced mitophagy dependent on its active site phosphohistidine but not the NDPK function.

17 using synthetic peptides, we conclude that 3-phosphohistidine cannot replace phosphotyrosine in confe

18 ced by glutamine, were phosphorylated by the phosphohistidine-containing phosphocarrier protein (HPr-

19 n the protein complex involving a 21-aa-long phosphohistidine-containing segment of the alpha subunit

20 transition-state (TS) analogue of enzymatic phosphohistidine dephosphorylation as an amino acid buil

21 reveals a three-domain molecule in which the phosphohistidine domain is flanked by the nucleotide and

22 ilitated by two conformational states of the phosphohistidine domain.

23 no acid that acts as a nucleophile forming a phosphohistidine-enzyme intermediate, and His(119) would

24 s limited overall homology to members of the phosphohistidine family of phosphatases.

25 We use the phosphohistidine immonium ion as a diagnostic tool as we

26 of a fairly strong hydrogen bond to the same phosphohistidine implies that hydrolysis of the covalent

27 The secreted enzyme forms a phosphohistidine intermediate and shows broad specificit

28 -phosphate during formation of the transient phosphohistidine intermediate at the N3' of His-258, thi

29 HMQC spectra acquired from the transient N3' phosphohistidine intermediate complex in the wild-type e

30 This five-coordinate phosphohistidine intermediate energetically exists betwe

31 ray crystal structure of the five-coordinate phosphohistidine intermediate from Streptomyces sp .

32 The phosphohistidine intermediate is characterized by two hy

33 Attempts to detect a phosphohistidine intermediate with the H256A mutant enzy

34 ydrolysis involved the formation of a stable phosphohistidine intermediate.

35 tose-2,6-bisphosphate, and subsequently, the phosphohistidine intermediate.

36 xylamine, suggesting that the enzyme forms a phosphohistidine intermediate.

37 The phosphohistidine is stabilized in the His432Arg structur

38 ohistidine phosphatase in mammals, regulates phosphohistidine levels of several proteins including th

39 Furthermore, it has been suggested that phosphohistidine might substitute for phosphotyrosine in

40 de indicate a covalent H247-BeF(3)(-) as the phosphohistidine mimic.

41 /Gly peptides containing the nonhydrolyzable phosphohistidine (pHis) analog- phosphotriazolylalanine

42 Still, the detection of phosphohistidine (pHis) in the proteome has remained dif

43 Phosphohistidine (pHis) was discovered 60 years ago as a

44 m the intrinsic instability and isomerism of phosphohistidine (pHis).

45 Phosphohistidine phosphatase 1 (PHPT1), the only known p

46 The peptides are designed as inhibitors of phosphohistidine phosphatase and as a pull-down probe fo

47 tidine phosphatase 1 (PHPT1), the only known phosphohistidine phosphatase in mammals, regulates phosp

48 sults support a tumor promoter role for LHPP phosphohistidine phosphatase in MDA-MB-231TNBC cells and

49 erate mutase family 5 (PGAM5) functions as a phosphohistidine phosphatase that specifically associate

50 We found that levels of the LHPP phosphohistidine phosphatase were significantly increase

51 ial therapeutic targets, we investigated the phosphohistidine phosphatase, LHPP, which has been impli

52 e for the existence of histidine kinases and phosphohistidine phosphatases has emerged, together with

53 d as a pull-down probe for identification of phosphohistidine phosphatases, respectively.

54 of the experimentally observed ''dead-end'' phosphohistidine product (PDB Code = 1V0W ).

55 favorability of the in vitro four-coordinate phosphohistidine product.

56 C cells did not significantly affect overall phosphohistidine protein levels.

57 ll allow more confident investigation of the phosphohistidine proteome to reveal the roles of histidi

58 (31)P NMR spectra were not consistent with a phosphohistidine residue.

59 Formation of phosphohistidine residues in proteins has been found in

60 (i.e., His760), which was proposed to form a phosphohistidine species during catalysis.

61 sphotransfer event; however, analysis of the phosphohistidine species is made difficult by the instab

62 ition to its activity on phosphotyrosine and phosphohistidine substrates, Tdp1 also possesses a limit

63 We have analyzed the ability of 3-phosphohistidine to associate with the known phosphotyro

64 ohistidine even when they do not form stable phosphohistidines using the natural substrate ATP.

65 utant resting enzymes were reversed when the phosphohistidine was formed, generating spectra very sim

66 y recognize the 1-pHis or 3-pHis isoforms of phosphohistidine were developed by immunizing rabbits wi