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1 d with caged rhodamine to make the particles photoactivatable.
3 ution CLIP (iCLIP), employing either UV-C or photoactivatable 4-thiouridine (4SU) combined with UV-A
4 constructed U25 snoRNAs containing a single photoactivatable 4-thiouridine at each U position within
5 e that exposes isolated white blood cells to photoactivatable 8-methoxypsoralen and ultraviolet A rad
7 eveloped an optogenetic strategy that uses a photoactivatable adenylate cyclase to achieve real-time
9 ted to fluorescein 5(6)-isothiocyanate, as a photoactivatable agent, followed by encapsulation in non
15 , we used 3-azibutanol and 3-azioctanol, the photoactivatable analogs of 1-butanol and 1-octanol, to
16 ides in mammalian brain tissue, we developed photoactivatable analogs of two opioids: [Leu(5)]-enkeph
18 of porcine SR with [(3)H]azidodantrolene, a photoactivatable analogue of dantrolene, has identified
20 Y720, accomplishing the first synthesis of a photoactivatable analogue of this ligand (2) in 9 steps
23 the lobatamides to mammalian V-ATPase, three photoactivatable analogues bearing benzophenone photoaff
26 e a new strategy that uses a novel synthetic photoactivatable and click chemistry-taggable phospholip
28 The thio group of 6-thio-ppGpp is directly photoactivatable and is thus a zero-length cross-linker.
29 und SecA to membrane lipids, a radiolabeled, photoactivatable and lipid-partitioning crosslinker, 3-t
30 nsively for biological imaging applications; photoactivatable and photoconvertible fluorescent protei
31 mechanism appears to be common to all of the photoactivatable and reversibly photoswitchable FPs repo
32 okes shift, fluorescent timers, irreversibly photoactivatable and reversibly photoswitchable RFPs.
33 ctivator to render it biologically inert and photoactivatable, and employing next-generation protein
34 analog trinitrophenol ATP, as well as with a photoactivatable ATP analog, 8-azido-ATP (N(3)-ATP).
36 either an electrophilic isothiocyanato or a photoactivatable azido group at the terminal carbon of t
37 ncluded the electrophilic isothiocyanate and photoactivatable azido groups, both of which are capable
39 rophosphonate inhibitor of FAAH containing a photoactivatable benzophenone group was synthesized and
42 riments demonstrate the covalent transfer of photoactivatable biotin from isolated cdb3-PO4 (but not
43 as used to direct the covalent attachment of photoactivatable biotin onto silylated silicon wafers.
54 tolabeled purified mouse VDAC1 (mVDAC1) with photoactivatable cholesterol analogues and analyzed the
55 are selectively targeted by the hydrophobic photoactivatable compound 1,5-iodonaphthyl-azide (INA).
56 vers organic and transition metal-containing photoactivatable compounds (complexes) that absorb in th
66 selectively with a maleimide to introduce a photoactivatable cross-linking aryl azide at three diffe
68 Biotin-terminated glycopolymers containing photoactivatable cross-linking groups were designed to c
69 onfocal fluorescence microscopy, and in vivo photoactivatable cross-linking of stalled translocation
74 new approach to produce genetically encoded photoactivatable derivatives of Rac1, a key GTPase regul
75 nd the utility of these dyes by synthesizing photoactivatable derivatives that are compatible with li
80 emically enabled volumetric 3D digital light photoactivatable dye display (3D Light PAD) is reported.
81 ration 3D Light PAD was fabricated using the photoactivatable dye N-phenyl spirolactam rhodamine B, a
82 ible and near-infrared light with a nontoxic photoactivatable dye, which upon encounter with molecula
84 metric dimers and oligomers using dual-color photoactivatable dyes and localization microscopy (PD-PA
85 he photophysical properties of commonly used photoactivatable dyes in a wide range of solvents, which
88 known 2 and 4, these FABPs comprise a set of photoactivatable fatty acid analogues with the crosslink
89 olism in cells, MBLAC2 was cross-linked to a photoactivatable fatty acid in a manner that was indepen
90 semiconducting pro-nanoenzyme (OSPE) with a photoactivatable feature for metastasis-inhibited cancer
91 s context, we designed molecular guests with photoactivatable fluorescence for these supramolecular h
93 than the width of the point spread function, photoactivatable fluorescent molecules are used, in this
95 d a transgenic mouse strain that expresses a photoactivatable fluorescent neurofilament protein in ne
97 d provide a versatile platform for designing photoactivatable fluorescent probes for various biologic
98 the tetrazole/alkene prefluorophores, robust photoactivatable fluorescent probes were obtained with f
99 limited availability of genetically encoded photoactivatable fluorescent probes with distinct emissi
100 strategies have been developed in generating photoactivatable fluorescent probes, most of which rely
101 a protocol that preserves both the delicate photoactivatable fluorescent protein labels essential fo
108 e, we evaluate the blinking kinetics of four photoactivatable fluorescent proteins for quantitative s
109 hotoactivation localization microscopy using photoactivatable fluorescent proteins genetically fused
111 reliance of modern microscopy techniques on photoactivatable fluorescent proteins prompted developme
115 enic strategy to measure cell division and a photoactivatable fluorescent reporter to examine whether
117 oped a facile and general strategy to obtain photoactivatable fluorogenic dyes across a broad spectra
118 tructural manipulations and describe a novel photoactivatable fluorophore based on a Si-containing an
119 aging with custom cell tracking software and photoactivatable fluorophore labeling to determine the c
121 imaging is in principle compatible with any photoactivatable fluorophore, it should have broad appli
125 imately, lead to the realization of valuable photoactivatable fluorophores for imaging applications.
126 rticle tracking of Nav channels labeled with photoactivatable fluorophores in combination with Bayesi
127 l PSF design and digital postprocessing with photoactivatable fluorophores opens up avenues for impro
128 demonstration provides a new class of bright photoactivatable fluorophores, as are needed for super-r
129 an be achieved by sequential localization of photoactivatable fluorophores, for which the image resol
130 utively active Rac1 or light activation of a photoactivatable form of Rac1 blocks the ability of repe
133 response to ligands, peptide cross-linked by photoactivatable free cholesterol (FCBP)], (iv) selectiv
135 populations of cytosolic proteins tagged to photoactivatable GFP (PAGFP) move with a slow motor-depe
141 Interestingly, expression of matrix-targeted photoactivatable GFP showed that mitochondrial fusion wa
142 analysis of peri-Golgi actin dynamics using photoactivatable GFP suggest a scenario in which TGN-loc
143 in cellula pulse-chase imaging protocol with photoactivatable GFP to investigate the mechanism underl
144 e to measure the rate of departure of PAGFP (photoactivatable GFP)-tagged neurofilaments from photoac
147 maging with a combination of FLIP, FRAP, and photoactivatable GFP-Bax, we demonstrate that disrupting
149 transport of cytosolic proteins (CPs) using photoactivatable GFP-PAGFP-with modifications of standar
150 e Rab5 membrane exchange rate, assessed with photoactivatable GFP-Rab5, decreases in the presence of
154 nd three of them are investigated here using photoactivatable GFP-tagged RLC (RLC-PAGFP) exchanged in
155 ce decay after photoactivation recordings of photoactivatable GFP-tagged tubulin that, despite this r
157 We report the development and application of photoactivatable Green Cherry (G(PA)C), the first geneti
158 Cultured sympathetic neurons expressing a photoactivatable green fluorescent neurofilament fusion
160 ve combined neural tracing using an improved photoactivatable green fluorescent protein (PA-GFP) with
161 rescent molecules are used, in this case the photoactivatable green fluorescent protein (PA-GFP).
162 horylatable PSD-95 mutant (T19A) tagged with photoactivatable green fluorescent protein (PAGFP) shows
165 used a dual, in vivo tagging strategy with a photoactivatable green fluorescent protein and externall
167 uorescence recovery after photobleaching and photoactivatable green fluorescent protein revealed that
169 In this issue, Victora et al. use imaging of photoactivatable green fluorescent protein to define the
170 e used localized, two-photon activation of a photoactivatable green fluorescent protein to investigat
171 PSD, we tagged synaptic proteins with PAGFP (photoactivatable green fluorescent protein) and used com
172 RESEARCH DESIGN AND Using matrix-targeted photoactivatable green fluorescent protein, mitochondria
173 either enhanced green fluorescent protein or photoactivatable green fluorescent protein, we have stud
175 first genetically encoded "continuously red-photoactivatable green" two-color probe for live cell im
176 tion was observed at superresolution using a photoactivatable-green-fluorescent-protein (PAGFP)-tagge
178 , containing p-benzoylphenylalanine (Bpa), a photoactivatable group, and biotin as a tag, were synthe
180 rivatives incorporating both fluorescent and photoactivatable groups still retained binding affinity
182 address this limitation, we have developed a photoactivatable HNO donor incorporating the (3-hydroxy-
183 port a synthetic immunology approach using a photoactivatable immune modulator (PIM) to increase Th17
184 One application based on enzyme-specific, photoactivatable inhibitors is to isolate a designated a
186 describe our recent result in engineering a photoactivatable intein compatible with living mammalian
187 alize the binding and adduction sites of the photoactivatable irreversible cocaine analog 3beta-(p-ch
188 transporter (DAT) was investigated using the photoactivatable irreversible cocaine analog [125I]3beta
190 even more specific interactions between the photoactivatable isoprenoid and any number of isoprenoid
191 n-protein recognition, we have synthesized a photoactivatable, isoprenoid-containing cysteine analogu
192 elieve the strategy can be extended to other photoactivatable ligands to characterize cell membrane r
195 omethyl)-3-(m-[125I]iodophenyl)-diazarene, a photoactivatable, lipophilic probe which promiscuously l
196 omethyl)-3-(m-[125I]iodophenyl)-diazarine, a photoactivatable, lipophilic probe, also did not result
198 500- to 750-nm sections with interferometric photoactivatable localization microscopy followed by sca
199 ere, single-particle tracking is paired with photoactivatable localization microscopy to observe the
202 omplexes were reacted with the bifunctional, photoactivatable maleimide reagent 4-(N-maleimido)benzop
206 ht, genetically expressed fluorescent marker photoactivatable monomeric (m)Cherry (PA-mCherry1) in co
208 ere, we report two bacteriophytochrome-based photoactivatable near-infrared fluorescent proteins, nam
211 ctive nucleotides were positioned beside the photoactivatable nucleotides, and cross-linking by brief
213 mber of a new class of SPOTs, small-molecule photoactivatable optical sensors of transmembrane potent
214 ns have driven the continuing development of photoactivatable or photoconvertible labels, including g
222 lipids by measuring the incorporation of the photoactivatable phosphatidylcholine analog 1-O-hexadeca
223 e have used hydrophobic photolabeling with a photoactivatable phosphatidylcholine analogue to detect
224 pattern was different from that reported for photoactivatable phospholipid binding to the nAChR.
226 We then describe the recently developed photoactivatable, photoswitchable, and reversibly photos
236 ion, readout and bleaching, large numbers of photoactivatable probes can be precisely localized to ob
237 maging techniques that apply localization of photoactivatable probes to achieve super-resolution.
241 ce recovery after photobleaching (iFRAP) and photoactivatable probes, coupled to biochemical experime
244 t-activated transition metal complexes offer photoactivatable prodrugs with novel targeted mechanisms
248 ch facilitates simultaneous imaging of three photoactivatable proteins in mammalian cells using fluor
252 sed target-specific azido DCDHFs, a class of photoactivatable push-pull fluorogens which produce brig
254 calized activation of a genetically encoded, photoactivatable Rac guanosine triphosphatase is suffici
255 rm and position and additionally show, using photoactivatable Rac transgenes, that it is the level of
257 ch we combined with cell-type-specific RNAi, photoactivatable Rac, and morphodynamic profiling, to in
260 we use zebrafish motor neurons expressing a photoactivatable Rac1 to co-opt endogenous growth cone g
261 robe, AS-PaRac1 (activated synapse targeting photoactivatable Rac1), that can label recently potentia
262 rescent calcium integrator calcium-modulated photoactivatable ratiobetric integrator (CaMPARI) report
263 rescent calcium integrator calcium-modulated photoactivatable ratiobetric integrator (CaMPARI) report
274 We also identified WIG1-binding motifs using photoactivatable ribonucleoside-enhanced crosslinking an
276 an transcripts that interact with eIF3 using photoactivatable ribonucleoside-enhanced crosslinking an
277 ing sites in plasma cells through integrated Photoactivatable-Ribonucleoside-Enhanced Cross-Linking a
278 combined AGO2 RIP-seq and AGO2 PAR-CLIP-seq (photoactivatable-ribonucleoside-enhanced cross-linking a
282 wn that ginkgolide derivatives with aromatic photoactivatable substituents are potent PAF receptor an
284 ubjected to a cross-linking screen using the photoactivatable, sulfhydryl-reactive reagent N-[4-(p-az
285 d to enhance the effective resolution of two photoactivatable/switchable fluorophore-labeled structur
287 sis of monomeric TagRFP, we have developed a photoactivatable TagRFP protein that is initially dark b
290 tatively shared Taxol/LPS receptor, a novel, photoactivatable Taxol analogue was employed to identify
291 mycomembrane-related proteins, we developed photoactivatable trehalose monomycolate analogues that m
292 hod known as "label transfer" that employs a photoactivatable trifunctional cross-linking reagent to
293 gn1 in CA1 mouse hippocampal neurons using a photoactivatable tyrosine kinase receptor (optoFGFR1).
294 nse suppression methodology to incorporate a photoactivatable unnatural amino acid and photochemicall
296 riments of cells expressing GFP-tubulin or a photoactivatable variant of GFP-tubulin, respectively, d
298 sed on split sfCherry2, we have engineered a photoactivatable variant that enables single-molecule lo
300 his mutation in mammalian cells we created a photoactivatable version of IDH2(R172K), in which K172 i