ライフサイエンスコーパス: photoaffinity

1 Detailed mapping of residues modified by the photoaffinity adducts may provide insight to guide the f

2 Using a photoaffinity agent, the molecular target has been ident

3 These have been characterized using the photoaffinity analog 8-azido-[alpha-32P]ATP as well as u

4 a molecular target for dantrolene using the photoaffinity analog [(3)H]azidodantrolene.

5 Experiments using a photoaffinity analog indicated that PSP binds to a singl

6 By using a photoaffinity analog of a potent respiratory syncytial v

7 st time, that [125I]iodoarylazidoprazosin, a photoaffinity analog of the substrate prazosin, labels m

8 Here, we utilize a photoaffinity analog of the VA isoflurane to identify a

9 5 nM, and an (125)I-labeled azido-Cys-AtPep1 photoaffinity analog specifically labeled a membrane-ass

10 Tritiated photoaffinity analogs of the natural lepidopteran juveni

11 was also specifically photolabeled by these photoaffinity analogs.

12 different numbers of cGMP moieties using the photoaffinity analogue 8-p-azidophenacylthio-cGMP; the r

13 We synthesized its photoaffinity analogue [(3)H](trifluoromethyl)phenyldiaz

14 We describe here the synthesis of the first photoaffinity analogue of SPC.

15 w here for the first time that both of these photoaffinity analogues are transport substrates for ABC

16 ]azidopine as well as the transport of these photoaffinity analogues by ABCG2.

17 Photoaffinity analogues of FPP (5, 6) were prepared by s

18 In this study, we show that photoaffinity analogues of HMBPP, meta/para-benzophenone

19 Two radiolabeled photoaffinity analogues of HTI-286, designated 4-benzoyl

20 The photoaffinity analogues were tested in a tubulin assembl

21 s for the production of analogues, including photoaffinity and biotinylated derivatives.

22 We show here, using photoaffinity and fluorescent derivatives, that this inh

23 Photoaffinity and radiolabeled analogues of MPC-6827 wer

24 ange of substituted aryl(CF(3))diazirines in photoaffinity applications.

25 These data validated the photoaffinity assay as an excellent system for the ident

26 fragment-screening platform, termed PhABits (PhotoAffinity Bits), which utilizes a library of photore

27 assinosteroids to BRI1 using a biotin-tagged photoaffinity castasterone (BPCS), a biosynthetic precur

28 binant PfMDR1 protein with a highly specific photoaffinity CQ analogue, and lack of competition for p

29 ntical levels of PS1 derivatives that can be photoaffinity cross-linked to a biotinylated, benzopheno

30 th a fluorescent probe Alexa Fluor 680 and a photoaffinity cross-linker APG) was cross-linked to RyR1

31 Two approaches were used--photoaffinity cross-linking and disulfide chemical cross

32 In this study, we employed a photoaffinity cross-linking approach to map the CR4/5-TR

33 cted based on experimental data derived from photoaffinity cross-linking by psoralen, phenphi (cis-Rh

34 Site-directed photoaffinity cross-linking experiments were performed b

35 minal halves of the MukB coiled coil through photoaffinity cross-linking experiments.

36 Phenanthroline-copper footprinting and photoaffinity cross-linking indicate that TFIID makes ex

37 Photoaffinity cross-linking of the template to the modif

38 tion of the SH groups of these residues with photoaffinity cross-linking reagents caused a significan

39 bserved activity as well as mutagenicity and photoaffinity cross-linking studies of the alpha(v)beta(

40 Photoaffinity cross-linking studies provided evidence fo

41 Importantly, previous photoaffinity cross-linking studies using a PTH analogue

42 Combining our results from photoaffinity cross-linking studies with data now availa

43 We demonstrate here the use of photoaffinity cross-linking to determine high-resolution

44 bination of site-directed mutagenesis and UV photoaffinity cross-linking, we have identified five ami

45 eavily on benzoylphenylalanine- (Bpa-) based photoaffinity cross-linking.

46 y disulfide trapping extends and complements photoaffinity cross-linking.

47 ished findings from receptor mutagenesis and photoaffinity cross-linking.

48 ts into integrin-ligand interactions through photoaffinity, cross-linking/mass spectroscopy, and X-ra

49 Previous photoaffinity crosslinking data are used to position the

50 the connector in the procapsid was found by photoaffinity crosslinking.

51 Here, we have designed and synthesized photoaffinity derivatives of the 4-acyl-1,6-dialkylpiper

52 R activation also promotes the binding of a photoaffinity GTP analog to a protein that migrates on o

53 The cocaine photoaffinity label 3-iodo-4-azidococaine ([125I]IACoc)

54 ine analogs, we developed a piperidine-based photoaffinity label [(125)I]4-[2-(diphenylmethoxy)ethyl]

55 the previously identified attachment of the photoaffinity label [(125)I]RTI 82 in TM6.

56 s well as receptor-mediated incorporation of photoaffinity label [(32)P]azidoanilido-GTP indicates hi

57 We previously prepared a benztropine-based photoaffinity label [125I]-(N-[4-(4'-azido-3'-iodophenyl

58 [125I]TBZ-AIPP were synthesized and used to photoaffinity label chromaffin granule membranes.

59 his new analogue was explored by using it to photoaffinity label crude protein extracts obtained from

60 Using a synthetic signal peptide harboring a photoaffinity label in its hydrophobic core, we examined

61 ular reactivity is a desirable quality for a photoaffinity label to possess, and thus, the resistance

62 Bpa is a photoaffinity label unnatural amino acid that can form c

63 rier-free, radioiodinated fenpropimorph-like photoaffinity label, 1-N-(2',6'-dimethyl-morpholino)-3-(

64 Herein, we report a new photoaffinity label, 2-aryl-5-carboxytetrazole (ACT), th

65 e spanning region labeled by a cocaine-based photoaffinity label, [125I] 2 (RTI 82).

66 os-4-yloxy l)-2-propylamine ([(3)H]ATB-BMPA) photoaffinity label.

67 Mass spectrometric analysis of photoaffinity labeled bNT-CRFR1 yielded a 1:1 complex wi

68 closed state, we identified the amino acids photoaffinity labeled by [(125)I]TID in the presence of

69 Three photoaffinity labeled derivatives of epothilone D were p

70 in and phosphoinositide binding protein, was photoaffinity labeled using a variety of benzophenone-co

71 scoveries have emboldened efforts to prepare photoaffinity-labeled and other unique forms of STX as p

72 Using a photoaffinity-labeled ascr#2 probe and amplified lumines

73 In the present study, photoaffinity-labeled discodermolide analogues are used

74 Three photoaffinity-labeled discodermolide analogues were synt

75 te phosphopeptide fragments corresponding to photoaffinity-labeled fragments that contain all interna

76 The identification of photoaffinity-labeled peptides was aided by a signature

77 compounds reported in this study selectively photoaffinity-labeled the CCK receptor, resulting in the

78 LY294002, reduced the ability of TGase to be photoaffinity-labeled with [alpha-(32)P]GTP, providing e

79 A binding ensemble profiling with (f)photoaffinity labeling (BEProFL) approach that utilizes

80 The photochemistry of a new photoaffinity labeling (PAL) agent, 5-azido-2-(N,N-dieth

81 n the global proteome is uniquely enabled by photoaffinity labeling (PAL) coupled with chemical enric

82 Interestingly, we find photoaffinity labeling (PAL) of the NACHT domain require

83 Photoaffinity labeling (PAL) was used to identify the bi

84 icate that this azide might be a very useful photoaffinity labeling agent, because the reactive inter

85 emonstrate the utility of these compounds as photoaffinity labeling analogues for the study of a vari

86 In this study, we used photoaffinity labeling and a proteomic approach to ident

87 Photoaffinity labeling and binding assays using transgen

88 In aggregate, results from the photoaffinity labeling and efflux assays using [(125)I]I

89 ral location of the site has been defined by photoaffinity labeling and electron crystallography, the

90 Photoaffinity labeling and fluorescence quenching experi

91 integrase (IN) inhibitor-binding site using photoaffinity labeling and mass spectrometric analysis.

92 sites of hTMPK inhibitors were validated by photoaffinity labeling and mass spectrometric studies.

93 eins were identified as filamin and actin by photoaffinity labeling and mass spectrometry.

94 This work focuses on the use of photoaffinity labeling and molecular modeling to elucida

95 in was confirmed by two separate approaches: photoaffinity labeling and site-specific antibodies.

96 The photoaffinity labeling and surface plasmon resonance-bas

97 es are also occupied, in a site suggested by photoaffinity labeling and thought to positively modulat

98 diazirines have achieved great popularity in photoaffinity labeling applications, the properties of t

99 t solution for all extant data, including 10 photoaffinity labeling constraints, a new such constrain

100 Combining the fluorescence data with photoaffinity labeling data provides insights into the c

101 tent with previous mutagenesis, chimera, and photoaffinity labeling data, demonstrating involvement o

102 When combined with previous photoaffinity labeling data, there are now seven indepen

103 based on experimental data from a series of photoaffinity labeling experiments and spectroscopic str

104 Although electron crystallography and photoaffinity labeling experiments determined that the b

105 BD1 can enhance the trapping of ADP at NBD2, photoaffinity labeling experiments with [alpha-(32)P]8-N

106 Recently, photoaffinity labeling experiments with mouse cell extra

107 tential drug binding site mimics and used in photoaffinity labeling experiments.

108 DNA photoaffinity labeling found that the Dpb4 subunit conta

109 Photoaffinity labeling identified two binding sites for

110 Photoaffinity labeling in a crude membrane fraction, fol

111 The present photoaffinity labeling in a physiologically relevant con

112 Photoaffinity labeling is a powerful tool to identify pr

113 Photoaffinity labeling is a useful technique employed to

114 entify the NAADP binding site, we employed a photoaffinity labeling method using a radioactive photop

115 This EET photoaffinity labeling method with a high signal-to-nois

116 elated and because the initial report of the photoaffinity labeling of a domain of this receptor incl

117 sin (IAAP) and [(3)H]azidopine were used for photoaffinity labeling of ABCG2 in this study.

118 ata demonstrating the efficient and specific photoaffinity labeling of CYP3A4 by this naturally occur

119 Further, the photoaffinity labeling of E969 indicated pore block as a

120 This is in contrast to photoaffinity labeling of FSH beta by the peptide mimic

121 Photoaffinity labeling of gamma-aminobutyric acid type A

122 e after sliding was also demonstrated by DNA photoaffinity labeling of histone proteins at specific s

123 Ing3A, but not PMA, blocked photoaffinity labeling of human P-gp with [(125)I]iodoar

124 Photoaffinity labeling of human protein geranylgeranyltr

125 We performed photoaffinity labeling of intact cells expressing fluore

126 Photoaffinity labeling of myofilament proteins with meta

127 mbination of whole cell transport assays and photoaffinity labeling of Pdr5p with [(125)I]iodoarylazi

128 Photoaffinity labeling of porcine SR with [(3)H]azidodan

129 g site is enriched in synaptic vesicles, and photoaffinity labeling of purified synaptic vesicles con

130 Photoaffinity labeling of Rab4 with [gamma-(32)P]GTP-azi

131 Photoaffinity labeling of Rab5 with [gamma-(32)P]GTP-azi

132 Photoaffinity labeling of receptors by bound agonists ca

133 DNA photoaffinity labeling of RSC showed that the Rsc4 subun

134 nicillenol analogue was also synthesized for photoaffinity labeling of target proteins.

135 Photoaffinity labeling of the AChR by (125)I-dizocilpine

136 h-affinity binding sites on the AChR; and 3) photoaffinity labeling of the AChR using (125)I-dizocilp

137 Photoaffinity labeling of the I-domain followed by LC/MS

138 In contrast, photoaffinity labeling of the M174A mutant using radioio

139 Photoaffinity labeling of the M181A mutant using radioio

140 bits in a concentration-dependent manner the photoaffinity labeling of the multidrug transporter with

141 rmediates formed during BER, we used a novel photoaffinity labeling probe and mouse embryonic fibrobl

142 6-azi-3-hydroxypregnan-20-one (6-AziP), as a photoaffinity labeling reagent to identify neuroactive s

143 studies using the 8-azidoadenosine family of photoaffinity labeling reagents.

144 efore consistent with the conclusions of the photoaffinity labeling results.

145 ce constraints were utilized along with nine photoaffinity labeling spatial approximation constraints

146 The use of a photoaffinity labeling strategy identified fumarate hydr

147 esent study, we used a unique chemoselective photoaffinity labeling strategy, the methionine proximit

148 proximation constraints coming from previous photoaffinity labeling studies and 12 distance restraint

149 y that appears consistent with findings from photoaffinity labeling studies and with site-directed mu

150 Photoaffinity labeling studies have demonstrated approxi

151 Previous photoaffinity labeling studies have placed the B site on

152 erol stereoisomers were further confirmed by photoaffinity labeling studies on G(s),G(i2), and G(i3)

153 Photoaffinity labeling studies show radiolabeling of sub

154 Finally, photoaffinity labeling studies showed an isoform-specifi

155 d cocaine analog recognition was verified in photoaffinity labeling studies using [(125)I]MFZ 2-24.

156 Photoaffinity labeling studies with the 3-azidobenzoic a

157 By photoaffinity labeling studies, we previously observed a

158 eptor has come from receptor mutagenesis and photoaffinity labeling studies, with both contributing t

159 oximation constraints identified in previous photoaffinity labeling studies.

160 er to select a suitable candidate for future photoaffinity labeling studies.

161 ng to closed and open state models of TRPA1, photoaffinity labeling suggested that the A-967079 cavit

162 The photochemistry of the widely used photoaffinity labeling system 4-amino-3-nitrophenyl azid

163 lize SA analogs in conjunction with either a photoaffinity labeling technique or surface plasmon reso

164 identifying the protein targets of MOMIPP by photoaffinity labeling techniques.

165 This is in contrast with previous photoaffinity labeling through positions 6, 18, 22, and

166 e receptor (nAChR), which have been shown by photoaffinity labeling to bind to a common site in the i

167 we have utilized the more direct approach of photoaffinity labeling to explore spatial approximations

168 In this work, we have used photoaffinity labeling to identify a critical spatial ap

169 ze novel photoreactive fusion inhibitors and photoaffinity labeling to obtain direct physical evidenc

170 Here we apply photoaffinity labeling using a propofol derivative, meta

171 By photoaffinity labeling using derivatives of apicularen a

172 Previous mutagenesis studies and photoaffinity labeling using ligand analogues suggested

173 m the dyad was shown by DNA footprinting and photoaffinity labeling using recombinant histone octamer

174 Photoaffinity labeling was also increased in the presenc

175 The photoaffinity labeling was light- and concentration-depe

176 Photoaffinity labeling was protected by a molar excess o

177 In addition, photoaffinity labeling was used to determine the spatial

178 binant CRALBP (rCRALBP) was characterized by photoaffinity labeling with 3-diazo-4-keto-11-cis-retina

179 tyrosine mutants within the binding site by photoaffinity labeling with 5-azido-6-chloropyridin-3-yl

180 TP binding to both peptides was confirmed by photoaffinity labeling with 8-azido-ATP that was increas

181 We have employed photoaffinity labeling with 8-azido-ATP, which supports

182 Tryptophan fluorescence quenching and direct photoaffinity labeling with [(14)C]halothane suggested a

183 pha1beta3 and alpha1beta3gamma2 GABA(A)Rs by photoaffinity labeling with [(3)H]21-[4-(3-(trifluoromet

184 Direct photoaffinity labeling with a full agonist probe confirm

185 Following photoaffinity labeling with all-trans-[11,12-(3)H]retino

186 Photoaffinity labeling with an epothilone A photoprobe l

187 ed and the hormone subunits were probed with photoaffinity labeling with receptor peptides correspond

188 pha4beta2 nAChRs was directly examined using photoaffinity labeling with the hydrophobic probe 3-(tri

189 atter conclusion based on previous data from photoaffinity labeling).

190 lationship data, including binding affinity, photoaffinity labeling, and acquired mutation in human c

191 Mutational analysis, photoaffinity labeling, and structural studies have prov

192 catalytic interactions using enzyme kinetic, photoaffinity labeling, and vanadate inhibition studies.

193 Photoaffinity labeling, chimera analysis, and mutagenesi

194 Using the combination of photoaffinity labeling, enzymatic digestion, MALDI-TOF a

195 ersus the completely unfolded state, we used photoaffinity labeling, hydrogen exchange, fluorescence

196 otein was supported by identification, using photoaffinity labeling, of a binding site for etomidate

197 Photoaffinity labeling, receptor site-directed mutagenes

198 specifically its binding site, which include photoaffinity labeling, site directed mutagenesis, and h

199 ibitor, and we identify its binding sites by photoaffinity labeling.

200 of subtype selectivity was examined here by photoaffinity labeling.

201 nucleosomal DNA regions by site-directed DNA photoaffinity labeling.

202 P and inositol phosphate (IP) induction, and photoaffinity labeling.

203 cking, isothermal titration calorimetry, and photoaffinity labeling.

204 leosomes were probed using site-specific DNA photoaffinity labeling.

205 of binding of motilin to its receptor using photoaffinity labeling.

206 nt quench of intrinsic STAS fluorescence and photoaffinity labeling.

207 ironment for use in structural studies using photoaffinity labeling.

208 unit interface consistent with azi-etomidate photoaffinity labeling.

209 The results indicate that these photoaffinity-labeling agents are binding at the same al

210 is makes diazirines potentially more general photoaffinity-labeling agents.

211 ino acid residues identified in two separate photoaffinity-labeling experiments, (3) structure-activi

212 , evidenced by mRNA and protein analyses and photoaffinity-labeling experiments.

213 Potential photoaffinity-labeling ligands of the PLG binding site w

214 Six different photoaffinity-labeling ligands were designed and synthes

215 4-azidobenzoyl and 4-azido-2-hydroxybenzoyl photoaffinity-labeling moieties were placed at opposite

216 ent with all existing structure-activity and photoaffinity-labeling studies.

217 Based on our previous studies using photoaffinity-labeling techniques in characterizing the

218 polymerase (L) protein that was validated by photoaffinity labelling-based target mapping.

219 Photoaffinity labels are powerful tools for dissecting l

220 DATs labeled with [(125)I]AD-96-129 or other photoaffinity labels displayed distinctive sensitivities

221 ]IAPEGlyMER and [125I]TBZ-AIPP are effective photoaffinity labels for VMAT2.

222 toactivatable analogues bearing benzophenone photoaffinity labels have been prepared.

223 e have been synthesized and characterized as photoaffinity labels of the vesicle monoamine transporte

224 a-1 and sigma-2 receptors, we show that both photoaffinity labels specifically and covalently derivat

225 ally positioned carrier-free, radioiodinated photoaffinity labels specifically designed to probe the

226 This is due to a lack of photoaffinity labels that are minimally modified from th

227 established total synthesis strategy and the photoaffinity labels were attached to the C26 hydroxyl g

228 ct that further development of this class of photoaffinity labels will lead to a broad range of appli

229 Traditional photoaffinity labels work through nonspecific C-H/X-H bo

230 iazirine and benzophenone, two commonly used photoaffinity labels, in two case studies ACT showed hig

231 be investigated using phosphorylnitrenes as photoaffinity labels.

232 rearrangements increases their usefulness as photoaffinity labels.

233 s sites involved in the binding of other DAT photoaffinity labels.

234 imic of exoloop 3 specifically and saturably photoaffinity-labels FSH alpha but not FSH beta.

235 ta2 nAChR at a single high-affinity site and photoaffinity-labels only the alpha4 subunit, presumably

236 ), in terms of their relative affinity for a photoaffinity ligand (2-azido-3-[(125)I]iodo-7,8-dibromo

237 d that the hAhR has a lower affinity for the photoaffinity ligand compared with mAhR(b-1).

238 The novel photoaffinity ligand N-[4-(4-azido-3-(125)I-iodophenyl)-

239 e, lapachenole has been used as an effective photoaffinity ligand of human P450 3A4, and mass spectro

240 Three photoaffinity ligands (PALs) for the human serotonin tra

241 While photoaffinity ligands (PALs) have been widely used to pr

242 ropofol binding sites have been mapped using photoaffinity ligands and mutagenesis; however, their pr

243 Tropane-based photoaffinity ligands covalently bind to discrete points

244 Photoaffinity ligands that were cross-linked to the rece

245 ounds of the 1,5-benzodiazepine CCK receptor photoaffinity ligands were originally prepared in an eff

246 eversible reactions, to be used as effective photoaffinity ligands.

247 d by use of mechanism-based inactivators and photoaffinity ligands.

248 Eight photoaffinity-modified residues were identified in rCRAL

249 22 bp fragment, we synthesized a (32)P- and photoaffinity moiety-labeled 22 bp dsRNA fragment and us

250 Using a bifunctional photoaffinity nucleotide analogue and a non-membrane-per

251 owever, BTN3A1 did not preferentially bind a photoaffinity prenyl pyrophosphate.

252 Therefore, we developed (1) a GSH-based photoaffinity probe (GSTABP-G) to target the "G site", a

253 cifically labeled by an active site-directed photoaffinity probe (III-63) for PS, indicating that the

254 ailored WOBE437-derived diazirine-containing photoaffinity probe (RX-055) irreversibly blocked membra

255 An angiotensin analog, photoaffinity probe 125I-SBpa-Ang II, was used to specif

256 gation of biotin to the C4S dodecasaccharide photoaffinity probe afforded a strategy for the isolatio

257 onist azidoepibatidine (AzEPI) prepared as a photoaffinity probe and radioligand.

258 ive site of gamma-secretase using a multiple photoaffinity probe approach called "photophore walking.

259 Both fluorescein and a benzophenone photoaffinity probe are incorporated, with total labelin

260 N-terminal fragment dimers exist by using a photoaffinity probe based on a transition state analog g

261 Moreover, enzyme inhibition kinetics and photoaffinity probe displacement experiments demonstrate

262 Here, we demonstrate that the photoaffinity probe E2012-BPyne specifically labels the

263 s-retinoic acid ([(3)H]9-cis-RA) as a direct photoaffinity probe for the characterization of human re

264 We have synthesized a state-dependent photoaffinity probe for the nicotinic acetylcholine rece

265 dine has been synthesized and evaluated as a photoaffinity probe for the putative transporter protein

266 biological evaluation of a novel paclitaxel photoaffinity probe is described.

267 Here we use a clickable photoaffinity probe to identify cathepsin D (CatD) as a

268 (TDBzcholine) was synthesized and used as a photoaffinity probe to map the orientation of an aromati

269 in the ion channel and that [(3)H]dFBr is a photoaffinity probe with broad amino acid side chain rea

270 riments using 100-fold excess unlabeled 2-5A photoaffinity probe, pApAp(8-azidoA), and authentic 2-5A

271 The newly synthesized, biotinylated photoaffinity probe, SQBAzide, was first shown to specif

272 Here we report the use of a novel photoaffinity probe, WC-21, to identify the sigma-2 rece

273 f concept study, we subsequently developed a photoaffinity probe-based competition assay to profile C

274 cleotide phosphate ([(32)P-5N(3)]NAADP) as a photoaffinity probe.

275 Here, we report that photoaffinity probes based on potent helical peptide inh

276 Chemical biology studies employing photoaffinity probes derived from ZCM-I-1 demonstrated i

277 We present a group of photoaffinity probes designed to bind to the family of C

278 n of KAT bisubstrate inhibitors to clickable photoaffinity probes enables the selective covalent labe

279 yl compounds 1 and 2 are therefore candidate photoaffinity probes for characterization of both mammal

280 ), were prepared in good yields as candidate photoaffinity probes for mammalian and insect nicotinic

281 ications for designing oligosaccharide-based photoaffinity probes for the identification of proteins

282 we report the first GAG polysaccharide-based photoaffinity probes for the system-wide identification

283 This hypothesis can be tested by using two photoaffinity probes that differ only in the N-unsubstit

284 By using photoaffinity probes to generate a relationship between

285 We designed and applied dynorphin-inspired photoaffinity probes to reveal the protein targets of th

286 ly of various chondroitin sulfate (CS)-based photoaffinity probes was developed.

287 ng the peptidoglycan biosynthetic machinery, photoaffinity probes were installed in combination with

288 substituents to obtain several affinity and photoaffinity probes which will be utilized in concert f

289 We developed clickable GSM photoaffinity probes with the goal of identifying the ta

290 ChBP by using optimized azidochloropyridinyl photoaffinity probes.

291 o active site lysines can be cross-linked to photoaffinity probes.

292 explore this, we examined the binding of the photoaffinity reagent 8-azido-ATP[gamma] biotin to purif

293 A system of photoaffinity reagents for selective labeling of DNA pol

294 rly demonstrate that chromenes are effective photoaffinity reagents for the cytochrome P450 superfami

295 Based on these results, we prepared photoaffinity reagents for the identification of the par

296 ave utilized two types of arylazido-modified photoaffinity reagents that probe residues in the Uvr pr

297 We used photoaffinity scanning (PAS) to identify key ligand-rece

298 The photoaffinity spin-labeled non-nucleoside ATP analogue,

299 ility to displace the substrate analogue and photoaffinity tag [(125)I]iodoarylazidoprazosin.

300 n of Rab5 with DATFP-FPP establishes a novel photoaffinity technique for the characterization of pren