ライフサイエンスコーパス: photobleach

1 the molecules are switched "off" rather than photobleached.

2 -dependent reactions, which usually cause PS photobleaching.

3 assumptions about the spectral dependency of photobleaching.

4 rature-dependent fluorescence recovery after photobleaching.

5 ation, and rapid fluorescence recovery after photobleaching.

6 intensity, the excess intensity just adds to photobleaching.

7 ed with PPIX fluorescence and degree of PPIX photobleaching.

8 s needs to be considered when correcting for photobleaching.

9 ements for finite filament length as well as photobleaching.

10 heir different fluorescence stability during photobleaching.

11 movement, low signal-to-background ratio and photobleaching.

12 spectroscopy and fluorescence recovery after photobleaching.

13 dic according to fluorescence recovery after photobleaching.

14 by the quantum yield of fluorophores and by photobleaching.

15 acteria cells achieved by regular SIRM after photobleaching.

16 asurements using fluorescence recovery after photobleaching.

17 aneously measuring fluorescence lifetime and photobleaching.

18 this artifact was measured using single-step photobleaching.

19 cle fusion using fluorescence recovery after photobleaching.

20 uper-resolution imaging with greatly reduced photobleaching.

21 itive and susceptible to photoinhibition and photobleaching.

22 polyp expansion, coral tissue reaction, and photobleaching.

23 ter cellular behavior and are susceptible to photobleaching.

24 uantified, using fluorescence recovery after photobleaching.

25 s to avoid photodamage to the cell and rapid photobleaching.

26 spectroscopy and fluorescence recovery after photobleaching.

27 s and generated a small fraction of opsin by photobleaching ~1% of rhodopsin.

28 applied a combination of biochemical assays, photobleaching/activation approaches, and atomistic mole

29 itions within a nucleus, without significant photobleaching, allowing us to make reliable estimates o

30 Additionally, fluorescence recovery after photobleaching analysis indicated impaired vimentin dyna

31 Fluorescence recovery after photobleaching analysis indicates that the paranodes pro

32 rgy transfer and fluorescence recovery after photobleaching analysis of postischemic brain endothelia

33 Interestingly, fluorescence recovery after photobleaching analysis reveals differential mobility of

34 trated in vitro, fluorescence recovery after photobleaching analysis suggests interactions in vivo ar

35 Here, we use single-molecule photobleaching analysis to measure the probability of Cl

36 Using fluorescence recovery after photobleaching analysis, we first show that secretory ve

37 Using fluorescence recovery after photobleaching analysis, we show that H1.3 has superfast

38 trans-ROL)) in the neural retina following a photobleach and 5-fold lower retinyl esters in the RPE.

39 nd the ERC using fluorescence recovery after photobleaching and a novel sterol efflux assay.

40 ability, as evidenced by suppression of both photobleaching and blinking behavior.

41 These probes are non-photobleaching and can be used alongside fluorophores wi

42 Secondary outcome measures were PPIX photobleaching and clinical local skin reactions, suppor

43 showed that SiRA is remarkably resistant to photobleaching and constitutes the brightest far-red lig

44 Common pitfalls such as photobleaching and cross-talk are addressed, as well as

45 Based on fluorescence recovery after photobleaching and endocytosis assays, integrin recyclin

46 copy techniques: fluorescence recovery after photobleaching and fluorescence correlation spectroscopy

47 al stress, using fluorescence recovery after photobleaching and fluorescence correlation spectroscopy

48 eurons employing fluorescence recovery after photobleaching and fluorescence correlation spectroscopy

49 The effect of scanning speed on photobleaching and fluorescence yield is more remarkable

50 terizations with fluorescence recovery after photobleaching and FRET corroborate the formation of mul

51 Photobleaching and genetic perturbations showed that the

52 racked in native terminals with simultaneous photobleaching and imaging (SPAIM) to show that DCVs und

53 ) is valuable for its combination of reduced photobleaching and outstanding spatiotemporal resolution

54 ften used as an acceptor but YFP is prone to photobleaching and pH changes.

55 Using photobleaching and pharmacological perturbations in vivo

56 Fluorescence photobleaching and photoactivation experiments also reve

57 Using photobleaching and photoconversion experiments in glial

58 formations, evaluate their stability against photobleaching and photoconversion in the context of oth

59 challenging for biological imaging as noise, photobleaching and phototoxicity compromise signal quali

60 nges in biological imaging include labeling, photobleaching and phototoxicity, as well as light scatt

61 ence-based voltage sensors often suffer from photobleaching and phototoxicity, which limit the record

62 To overcome issues with photobleaching and poor distinction between confocal and

63 y, we demonstrate using fluorescence loss in photobleaching and quantitative co-localization with chr

64 sessed by slowed fluorescence recovery after photobleaching and resistance to salt.

65 Meanwhile, the unique photobleaching and signal-to-noise benefits afforded by

66 cytoplasmic oligomerization, while stepwise photobleaching and single molecule colocalization may be

67 ity of the method and the demonstration that photobleaching and the photophysical properties of the d

68 rials are often used for their resistance to photobleaching and their complex viewing-direction-depen

69 tamate uncaging, fluorescence recovery after photobleaching and transgenic mice expressing labeled PS

70 : A fluorescent dextran inside TATS lumen is photobleached, and signal recovery by diffusion of unble

71 of round cells, fluorescence recovery after photobleaching, and a mathematical mean-field model of c

72 s illumination light to photoswitch off than photobleaching, and can be photoswitched "on" again to r

73 ng of single fluorescent proteins, step-wise photobleaching, and multiparameter spectroscopy, allows

74 ve-cell imaging, fluorescence recovery after photobleaching, and single molecule tracking showed that

75 eling protocols, fluorescence recovery after photobleaching, and single particle tracking.

76 ation was subsequently reduced by additional photobleaching, and the diffusion of individual SRB mole

77 scent microscopy, fluorescent recovery after photobleaching, and transmission electron microscopy, th

78 In contrast, photobleaching anterograde transport vesicles entering a

79 ily because of the difficulty of determining photobleaching apparent quantum yields (AQYs) that captu

80 present a simple method to determine a CDOM photobleaching AQY matrix (AQY-M) for natural water samp

81 ons by employing fluorescence recovery after photobleaching as an in vivo assay to measure the influe

82 obabilities, in conjunction with fluorophore photobleaching assays on over 2000 individual complexes,

83 se PolC functions in B. subtilis, we applied photobleaching-assisted microscopy, three-dimensional su

84 tions, the need for precise knowledge of the photobleach beam profile, potential for bias due to samp

85 ciation, we used fluorescence recovery after photobleaching beam-size analysis to study the membrane

86 ti-colour emission process, and blinking and photobleaching behaviours of single tetrapods can be con

87 ever, long maturation times, low brightness, photobleaching, broad emission spectra, and sample autof

88 Our results show that photobleaching can critically skew the estimation of oli

89 However, photobleaching can occur before the sample is appropriat

90 sunlight will rapidly and non-destructively photobleach CDs into optically inactive carbon nanoparti

91 hoton excitation (2PE) and poorly understood photobleaching characteristics have made their implement

92 le group, about twice the amount of PPIX was photobleached compared to topical cream application.

93 MG complexes emit 2-fold more photons before photobleaching compared to organic dyes such as Cy5 and

94 rwent fusion and fluorescence recovery after photobleaching consistent with the PRD LLPS in vitro.

95 for analysis of fluorescence recovery after photobleaching data.

96 with the dynactin disruptor mycalolide B or photobleaching DCVs entering a synaptic bouton by retrog

97 Fluorescence recovery after photobleaching demonstrates that P(DeltaOD) diffuses 6-f

98 h-RAM computer (64 Gb) to run and includes a photobleaching detrending algorithm, which allows extens

99 essed by calcein fluorescence recovery after photobleach during intracellular [Ca] recording.

100 tation times, thereby drastically decreasing photobleaching during repeated scanning.

101 enzyme-dependent fluorescence recovery after photobleaching (ED-FRAP) of NADH has been shown to be an

102 No notable photobleaching effect or degradation could be observed d

103 these MgPzs (with the highest and the lowest photobleaching efficiencies), we found that the higher t

104 integrin tensions and can be switched off by photobleaching, enabling continuous real-time imaging of

105 a combination of high intensity UV pulses to photobleach epicardial NADH.

106 Photobleaching event counting is a single-molecule fluor

107 uorophores, and the possibility that several photobleaching events occur almost simultaneously.

108 ddition to complications such as overlapping photobleaching events that may arise from fluorophore in

109 ross-linking and fluorescence recovery after photobleach experiments, and it helps resolve the long d

110 e microscopy and fluorescence recovery after photobleaching experiments and found that mycomembrane f

111 Fluorescence recovery after photobleaching experiments demonstrated that subunit exc

112 te, we performed fluorescence recovery after photobleaching experiments in living cells, which expres

113 in reporters and fluorescence recovery after photobleaching experiments in zebrafish embryos identifi

114 Photobleaching experiments indicated that co-assembly of

115 Mechanistically, fluorescence-recovery-after-photobleaching experiments point for the upstream role o

116 Fluorescence-recovery-after-photobleaching experiments revealed that Kv2.1, VAPA, an

117 Single-molecule fluorescence photobleaching experiments revealed that PopD formed mos

118 These data, together with photobleaching experiments to measure nucleolar protein

119 In summary, any inference from photobleaching experiments with B > 0.1 is likely to be

120 Through fluorescence recovery after photobleaching experiments, many components of the AMR h

121 complemented by fluorescence recovery after photobleaching experiments, which reveal an inverse corr

122 ere monitored by fluorescence recovery after photobleaching experiments.

123 pool with kinetics similar to those seen in photobleaching experiments.

124 id dynamics with fluorescence recovery after photobleaching experiments.

125 Fluorescence loss in photobleaching (FLIP) and network analysis experiments r

126 e (the largest TIRF depth) to preferentially photobleach fluorescence from the lower layers and allow

127 is combined with fluorescence recovery after photobleaching, fluorescence correlation spectroscopy an

128 We use fluorescence recovery after photobleaching, fluorescence correlation spectroscopy, a

129 tion spectroscopy to quantify the diffusion, photobleaching, fluorescence intermittency, and photocon

130 The RIT, defined as the time taken after a photobleach for visual sensitivity to recover detection

131 agellar transport particles, or reduction of photobleaching for live microtubule imaging.

132 vo validation of interactions using acceptor photobleaching Forster resonance energy transfer and flu

133 wledge, no other fluorescence recovery after photobleaching framework incorporates all these model fe

134 Moreover, fluorescence recovery after photobleaching (FRAP) analysis demonstrated that exposur

135 ealed ghosts and fluorescence recovery after photobleaching (FRAP) analysis of actin filament mobilit

136 s we show, using fluorescence recovery after photobleaching (FRAP) and fluorescence anisotropy measur

137 Using fluorescence recovery after photobleaching (FRAP) and single-molecule tracking in hu

138 oscopy (FCS) and fluorescence recovery after photobleaching (FRAP) are widely used methods to determi

139 Fluorescence recovery after photobleaching (FRAP) assays revealed that the GFP-MuMx1

140 opy imaging, and fluorescence recovery after photobleaching (FRAP) assays, we show that the divalent

141 experiments with fluorescence recovery after photobleaching (FRAP) confirmed the active vesicle traff

142 Fluorescence recovery after photobleaching (FRAP) experiments confirmed the differen

143 lopment in 1976, fluorescence recovery after photobleaching (FRAP) has been one of the most popular t

144 es by monitoring fluorescence recovery after photobleaching (FRAP) in transgenic zebrafish with GFP-t

145 Fluorescence recovery after photobleaching (FRAP) is a well-established experimental

146 Fluorescence recovery after photobleaching (FRAP) is an excellent tool to measure th

147 Fluorescence recovery after photobleaching (FRAP) is widely used to assess condensat

148 scent probe from Fluorescence Recovery After Photobleaching (FRAP) measurements assumes bleaching wit

149 Fluorescence recovery after photobleaching (FRAP) microscopy is used to probe the di

150 and the standard fluorescence recovery after photobleaching (FRAP) model introduced by Axelrod et al.

151 emonstrated using fluorescent recovery after photobleaching (FRAP) monitoring displacement of GFP-BAZ

152 and using a dual fluorescence recovery after photobleaching (FRAP) reporter assay for axonal translat

153 Interestingly, fluorescence recovery after photobleaching (FRAP) results indicated that NKKY101 mut

154 Notably, fluorescence recovery after photobleaching (FRAP) shows that YscQ exchanges between

155 pid assessments, fluorescence recovery after photobleaching (FRAP) static laser microscopy, and deter

156 Fluorescence recovery after photobleaching (FRAP) studies indicate that like H1, bin

157 cribe the use of fluorescence recovery after photobleaching (FRAP) to probe chain mobility in reversi

158 oscopy (HS-AFM), fluorescence recovery after photobleaching (FRAP), confocal laser scanning microscop

159 chniques such as fluorescence recovery after photobleaching (FRAP), fluorescence correlation spectros

160 We performed fluorescent recovery after photobleaching (FRAP), quantitative RT-PCR, and whole ce

161 Using fluorescence recovery after photobleaching (FRAP), we demonstrate that adherens junc

162 application, and Fluorescence Recovery After Photobleaching (FRAP).

163 established but underutilized method called photobleaching FRET (pbFRET), with the major difference

164 species with one of the acceptors absent or photobleached, from which two-color FRET data is collect

165 ourier transform fluorescence recovery after photobleaching (FT-FRAP) with patterned illumination is

166 Perturbation of equilibrium distributions by photobleaching has also been developed into a robust met

167 Single-molecule photobleaching has emerged as a powerful non-invasive ap

168 imaging can partially overcome the limits of photobleaching; however, limitations of this technique r

169 ncluding inverse fluorescence recovery after photobleaching (iFRAP) and photoactivatable probes, coup

170 Fluorescence recovery after photobleaching imaging reveals that the S561A mutant sho

171 e complex can be revealed by single-molecule photobleaching imaging.

172 Photoconversion, photobleaching, immunofluorescence and super-resolution

173 OM), which are widely distributed but highly photobleached in the surface ocean, are critical in regu

174 uorescent nanodiamonds (FNDs) show almost no photobleaching in a physiological environment.

175 scent signals in the presence of fluorophore photobleaching in a solid surface bioassay.

176 butable to the absence of phototoxicity, and photobleaching in bioluminescent imaging, combined with

177 been considered an effective means to reduce photobleaching in fluorescence microscopy, but a careful

178 spectroscopy and fluorescence recovery after photobleaching in HeLa cells.

179 s and studies of fluorescence recovery after photobleaching in respiratory mucus showed that mechanis

180 Fluorescent recovery after photobleaching in slices from VGLUT1(Venus) knock-in mic

181 sterol probe, is combined with resistance to photobleaching in solution and in human fibroblasts and

182 Our results therefore indicate that photobleaching is a necessary step toward inflicting irr

183 Single molecule photobleaching is a powerful technique to measure the nu

184 theoretically that speckle imprinting using photobleaching is optimal when the laser energy and fluo

185 We confirm that STED Photobleaching is primarily caused by the depletion ligh

186 labels (i.e., maximum emitted photons before photobleaching) is a critical requirement for achieving

187 ticles in a region of interest by repeatedly photobleaching its boundary.

188 tachment to proteins, have a ~54-fold higher photobleaching lifetime and emit ~43-fold more photons t

189 ameters were optimized to deliver 23.8 mJ of photobleaching light energy at a pulse width of 6 msec a

190 er they possess narrow Stokes shifts and can photobleach, limiting multiplexed detection applications

191 Photobleaching limits extended imaging of fluorescent bi

192 st, we visualized whole eisosomes and, after photobleaching, localized recruitment of new Pil1p molec

193 s in cancer phototherapy is often limited by photobleaching, low tumor selectivity, and tumor hypoxia

194 In addition, their strong resistance to photobleaching makes them suitable for long duration or

195 Fluorescence recovery after photobleaching measurements revealed that single mismatc

196 A detailed investigation of the photobleaching mechanism of these porphyrazines revealed

197 ne by using FRET microscopy and the acceptor photobleaching method.

198 cted by dye concentration, light scattering, photobleaching, micro-viscosity, temperature, or the mai

199 Using fluorescence recovery after photobleaching microscopy, we show that apparent speed o

200 Thus, as fluorophores stochastically photobleach, noise properties of the time trace change s

201 vant information that may be acquired before photobleaching occurs.

202 ed probe DNA on these surfaces is unlabeled, photobleaching of a probe label is not an issue, allowin

203 t absorbing, highlighting the possibility of photobleaching of BrC during their atmospheric aging and

204 The photobleaching of chromophoric dissolved organic matter

205 sensitive cells with no phototoxicity and no photobleaching of fluorescent biomarkers.

206 The system utilizes photobleaching of fluorophore dyes in the bulk flow and

207 ic receptor (M2R) and Gi1 by single-particle photobleaching of immobilized complexes.

208 en incorporated into phospholipid membranes, photobleaching of MgPzs correlates with the degree of li

209 umbers of molecules from fluctuations in the photobleaching of proteins tagged with Green Fluorescent

210 In the other approach, fractional photobleaching of reference oligomers provides a novel p

211 ation, the fundamental molecular event after photobleaching of rhodopsin is the recombination reactio

212 hat PKM2 phosphorylation is signaled through photobleaching of rhodopsin.

213 s technique remain present such as the rapid photobleaching of several types of organic fluorophores

214 Stepwise photobleaching of SpoIVFB fused to a fluorescent protein

215 Fluorescence recovery after photobleaching of tagged Nexus-associated proteins showe

216 We demonstrate that after photobleaching, (*)OH exposure degrades CDs in a two-ste

217 d subsequently observing as the fluorophores photobleach, one obtains information on the number of su

218 scopy as well as fluorescence recovery after photobleaching or photoswitching, and observed significa

219 y of an optical probe without suffering from photobleaching or phototoxicity.

220 via competitive absorption, and as a result, photobleaching or side reactions of the fluorophore are

221 s offer a superior optical signal and do not photobleach, our novel protocol holds enormous promise o

222 l neurons, using fluorescence recovery after photobleaching, particle tracking, and modeling.

223 nalysis of multiple spatial harmonics of the photobleaching pattern.

224 The photobleaching patterns of eGFP-M2R were indicative of a

225 rastructure, and fluorescence recovery after photobleaching/photoconversion experiments showed that t

226 However, photobleaching, phototoxicity, and related artifacts con

227 ted polymer to avoid leakage or differential photobleaching problems existed in other nanoprobes.

228 ial Fourier domain removed dependence on the photobleach profile, suppressing bias from imprecise kno

229 aging, including fluorescence recovery after photobleaching, provided further support for the role of

230 Single-molecule fluorescence recovery after photobleaching provides direct measurement of elongation

231 hotophysical parameters of the probe such as photobleaching quantum yield, count rate per molecule, a

232 inetic model to determine photoreduction and photobleaching rate constants.

233 uorescence signal, photoconversion rate, and photobleaching rate of mEos3.2 sensitive to the buffer c

234 tended times is challenging due to the rapid photobleaching rate of most fluorophores.

235 tive redox reactions that contributed to the photobleaching rate were studied over a wide temperature

236 amples and provide constraints when modeling photobleaching rates in natural waters.

237 coherent and optimized for modeling accurate photobleaching rates in natural waters.

238 ntegrated signal and the photoconversion and photobleaching rates of fluorescent proteins in cells.

239 ter simulations, fluorescence-recovery-after-photobleaching recovery times of both fused and single-m

240 her increasing the linear scanning speed for photobleaching reduction in STED microscopy.

241 Photobleaching remains a limiting factor in superresolut

242 ion coefficient, low quantum yield, and high photobleaching resistance.

243 tremely low luminescence background and high photobleaching resistance.

244 Fluorescence recovery after photobleaching revealed that the viscosity of CF ASL was

245 Fluorescence recovery after photobleaching reveals reversible changes in the level o

246 s include mechanical uncertainties, specimen photobleaching, segmentation, and stitching inaccuracies

247 xperiments using fluorescence recovery after photobleaching show that human FZD4 assembles-in a DVL-i

248 ealed that the GFP-MuMx1 nuclear bodies upon photobleaching showed a slow partial recovery (mobile fr

249 Stepwise photobleaching showed that CaMKII formed oligomeric comp

250 The analysis of fluorescence recovery after photobleaching showed that the fluxes of dye molecules i

251 Fluorescence recovery after photobleaching shows that TSSC1 is required for efficien

252 ingle melanosome fluorescence recovery after photobleaching (smFRAP) to characterize the association

253 gy to perform single-molecule recovery after photobleaching (SRAP) within dense macromolecular assemb

254 sian analysis of images collected during the photobleaching step of each plane enabled lateral superr

255 Although photobleaching steps are often detected by eye, this met

256 Our method is capable of detecting >/=50 photobleaching steps even for signal-to-noise ratios as

257 orithms, it is possible to reliably identify photobleaching steps for up to 20-30 fluorophores and si

258 ties have created a challenge in identifying photobleaching steps in a time trace.

259 factors can limit and bias the detection of photobleaching steps, including noise, high numbers of f

260 While fluorescence recovery after photobleaching studies demonstrated that ROP dynamics do

261 Through photobleaching studies, we show that BMs are not static-

262 linked Orai1 concatemers and single-molecule photobleaching suggest that channels assemble as tetrame

263 A-wave recovery compared with WT mice after photobleaching, suggesting a delayed dark adaptation.

264 Using fluorescence recovery after photobleaching technique, diffusion coefficients D of fl

265 her, field synthesis achieves lower rates of photobleaching than light sheets generated by lateral be

266 ters are frequently degraded by blinking and photobleaching that arise from poorly passivated host cr

267 CDOM origin (terrestrial versus marine) and photobleaching that controls variations in AQYs, with a

268 crease of fluorescence anisotropy seen after photobleaching the candidates.

269 es), we found that the higher the rate of PS photobleaching the faster the leakage induced in the mem

270 excitation zone by sequentially imaging and photobleaching the fluorescent molecules.

271 w blinking characteristics due to reversible photobleaching, the blinking of GNPs seems to be stable

272 After photobleaching, the EB1 signal at the flagellar tip reco

273 Irradiation with laser light above the photobleaching threshold induces photonic confinement po

274 icroscope enabled patterned illumination for photobleaching through two-photon excitation.

275 The noise properties in a photobleaching time trace depend on the number of active

276 t a new Bayesian method of counting steps in photobleaching time traces that takes into account stoch

277 Here, we applied single-molecule photobleaching to analyze the oligomeric state of an end

278 a procedure for fluorescence recovery after photobleaching to examine dye leakage through bacterial

279 the anthropogenic source shows a shift from photobleaching to photohumification denoted by an increa

280 we use fluorophore localization imaging with photobleaching to probe the structure of EGFR oligomers.

281 including using chemical cleavage instead of photobleaching to remove fluorescent signals between con

282 st successful application of single-molecule photobleaching to resolve drug-induced and domain-depend

283 g a three-state model of photoconversion and photobleaching to the time course of fluorescence signal

284 uch as strong signal strength, resistance to photobleaching, tunable fluorescence emissions, high sen

285 r, near-membrane fluorescence recovery after photobleaching, uncaging or photoactivation/switching as

286 ment of photosynthetic efficiency and became photobleached under high light (HL) growth conditions.

287 Importantly, CyB suffers photobleaching under a Nd:YAG laser but the signal decre

288 DA was assessed at baseline in 1 eye after a photobleach using a computerized dark adaptometer with t

289 The technique of Fluorescence Recovery After Photobleaching was applied for the first time on real ch

290 ured by 2-photon fluorescence recovery after photobleaching, was not affected just after cardiorespir

291 Through live-cell photobleaching, we find rapid binding kinetics between P

292 Using fluorescence loss in photobleaching, we find that the endoplasmic reticulum (

293 urthermore using fluorescence recovery after photobleaching, we found that FAK inhibition increased t

294 studying the recovery of fluorescence after photobleaching, we found that there are three mantATP bi

295 Using fluorescence recovery after photobleaching, we show that an ACTN4 mutation that caus

296 Using fluorescence recovery after photobleaching, we show that the ER chaperone Kar2/BiP f

297 or wood smoke BrC, both photoenhancement and photobleaching were observed.

298 physical problems of acceptors such as rapid photobleaching, which is crucial for high time resolutio

299 dark adaptation was assessed in 1 eye after photobleach with targets centered at 5 degrees on the in

300 per molecule, the saturation intensity, the photobleaching yield, and, crucially, management of brig