ライフサイエンスコーパス: photolabel

1 llular loop region that it has been shown to photolabel.

2 elective general anesthetic and an effective photolabel.

3 d state) and then rapidly frozen (<1 ms) and photolabeled.

4 contribute to the pool's architecture can be photolabeled.

5 roactive steroids inhibited etomidate analog photolabeling.

6 istance (CQR) affect the efficiency of AzBCQ photolabeling.

7 effect of phencyclidine (PCP) on [(125)I]TID photolabeling.

8 cid residues of the receptor for [125I]IACoc photolabeling.

9 Rs) were studied using electrophysiology and photolabeling.

10 , and top-down MS confirmed a single site of photolabeling.

11 and indinavir effectively protected against photolabeling.

12 econds after mixing, by use of time-resolved photolabeling.

13 ulator, which neither enhanced nor inhibited photolabeling.

14 The hydrophobic photolabel 3-trifluoromethyl-3-(m-[(125)I]iodophenyl)dia

15 The photolabeled 35-kDa protein was isolated from rat brain

16 yrrolidinediol (8-N(3)-ADP-HPD), was used to photolabel a recombinant bovine PARG catalytic fragment

17 EBDA and EHDA specifically photolabeled a 29-kDa nuclear protein (pI 5.8).

18 We photolabeled a myelin-enriched fraction from rat brain w

19 ta-tubulin and [(3)H]2-(m-azidobenzoyl)Taxol photolabels a peptide containing amino acid residues 217

20 After channel opening, TID photolabels a residue on the delta-subunit's M2-M3 loop

21 rified alpha4beta2 nAChRs, [(3)H]epibatidine photolabeled alpha4Tyr(195) (equivalent to Torpedo alpha

22 a site at the gamma-alpha subunit interface, photolabeling alphaM2-10 (alphaSer-252) and gammaMet-295

23 te); and (iii) at the gamma-alpha interface, photolabeling alphaM2-10'.

24 brane domain outside the ion channel, but it photolabels alphaMet-386 and alphaSer-393 in the cytopla

25 tylcholine binding sites, [(3)H]azietomidate photolabeled alphaTyr-93, alphaTyr-190, and alphaTyr-198

26 t sites within the alpha and delta subunits, photolabeling alphaVal-218 (alphaM1), deltaPhe-232 (delt

27 Photolabeled amino acid residues in each M4 segment were

28 Positioning these two photolabeled amino acids in a single type of binding sit

29 Photolabeled amino acids in both subunits were identifie

30 Consideration of the location of the photolabeled amino acids in homology models of the nAChR

31 determined by Edman degradation some of the photolabeled amino acids in nAChR subunit fragments isol

32 Photolabeled amino acids in the agonist binding sites we

33 In the presence of agonist, [(3)H]dFBr also photolabeled amino acids in the nAChR extracellular doma

34 The locations of the photolabeled amino acids in the nAChR structure and the

35 nce of agonist (carbamylcholine), both drugs photolabeled amino acids on the complementary (non-alpha

36 dine and identified by Edman degradation the photolabeled amino acids.

37 nds at the extracellular end of the channel, photolabeling amino acids at positions M2-16 (alpha,gamm

38 n gammaM3, and to a site in the ion channel, photolabeling amino acids within each subunit M2 helix t

39 ilize the nAChR in a closed state, [(3)H]CPZ photolabels amino acids at M2-5 (alpha), M2-6 (alpha,bet

40 Its photolabel analog [(3)H]azi-etomidate labels residues wi

41 a-tubulin isotypes, bovine brain tubulin was photolabeled and the isotypes resolved by high-resolutio

42 d mitochondria, individual mitochondria were photolabeled and tracked through fusion and fission.

43 he pivotal reactive intermediate involved in photolabeling and cross-linking studies using the 8-azid

44 gA in DMPC bilayers, direct [(14)C]halothane photolabeling and microsequencing demonstrated dominant

45 or and hence should be promising ligands for photolabeling and subsequent sequencing studies.

46 oflurane binding sites were identified using photolabeling and were further validated by the docking

47 hR binding moiety, a benzophenone moiety for photolabeling, and an alkyne moiety for biotinylation vi

48 differential scanning calorimetry and lipid photolabeling, and measured the affinity of this interac

49 ,1'-binaphthyl-5,5'-disulfonic acid (BisANS) photolabeling approach to monitor changes in protein unf

50 In this study, we adapted a UV photolabeling approach, using an apolar fluorescent prob

51 nd subunit selectivity of [(3)H]azietomidate photolabeling are discussed in terms of the structures o

52 ids enhance rather than inhibit azietomidate photolabeling, as assayed at the level of GABA(A)R subun

53 ed His-36, whereas its isomer, 7-azioctanol, photolabeled Asp-41.

54 A photolabeling assay showed that this annexin could bind

55 ATPase and photolabeling assays demonstrated that compounds with ri

56 oreover, the PS1 heterodimer is specifically photolabeled at the cell surface by a potent inhibitor t

57 Propofol enhanced [(3)H]AziPm photolabeling at alphaM2-10'.

58 The photolabeling at the cytoplasmic end of the channel is f

59 fen, whereas neither drug inhibits [(3)H]CPZ photolabeling at the extracellular end, establishing tha

60 Although the efficiency of photolabeling at the subunit level was unaffected by ago

61 and that [(3)H]azidopine can also be used to photolabel both wild-type R482-ABCG2 and mutant T482-ABC

62 Consistent with this, E193 was photolabeled by 3-azibutanol.

63 3 and alpha1Met-236 in alpha1M1), previously photolabeled by [(3)H]azietomidate, and alpha1Ile-239, l

64 When the amino acids photolabeled by [(3)H]AziPm were identified by protein m

65 ino acids of each nAChR subunit specifically photolabeled by [(3)H]tetracaine that contribute to the

66 The proteins photolabeled by [(32)P-5N(3)]NAADP have molecular masses

67 olated, detergent-solubilized GluT1.HA.H6 is photolabeled by [gamma-32P]-azidoATP in an ATP-protectab

68 o acid in the alpha1-beta3 subunit interface photolabeled by R-[(3)H]mTFD-MPAB.

69 des (or limited sequences) site-specifically photolabeled by radioactive photolabile oligoDNA probes

70 rface involving gammaTrp-453 (M4), a residue photolabeled by small lipophilic probes and promegestone

71 -kDa cytosolic protein was also specifically photolabeled by these photoaffinity analogs.

72 ent is close enough to the active site to be photolabeled by trapped ATP analogues.

73 acetyl-geranylgeranyl cysteine enhanced E193 photolabeling by 3-azibutanol.

74 how reserpine- and tetrabenazine-protectable photolabeling by [125I]IAmF.

75 mer-causing mutation in PS1 strongly reduced photolabeling by a transition-state analogue but not by

76 ncentration dependence of inhibition of that photolabeling by etomidate or R-mTFD-MPAB also establish

77 tates by using electrophysiology-coordinated photolabeling by several lipophilic probes followed by m

78 ity CQ analogue, and lack of competition for photolabelling by VP, supports our QTL predictions.

79 dines are substrates of ABCG2 and that these photolabels can be used to screen new substrates and/or

80 Incubation of [125I]TBZ-AIPP-photolabeled chromaffin granule membranes in the presenc

81 a32P]ATP-nucleotidylated or [alpha32P]8N3ATP-photolabeled CK is treated with trypsin a single, identi

82 Photolabeled CRABP-I was hydrolyzed with endoproteinase

83 Photolabeled CYP3A4 peptide adducts were further charact

84 These photolabeling data suggest that an accessory component w

85 They also photolabeled deltaTyr-212 at the delta-beta subunit inte

86 (3)) resulted in complete protection against photolabeling, demonstrating that [(32)P]pApAp(8-azidoA)

87 had normal ATPase activity, indicating that photolabeling did not significantly alter the enzymatic

88 t that the ability of NANTP and SSL-NANTP to photolabel different sites results from different orient

89 mately 0.5 mol of (14)C/mol of subunit, with photolabeling distributed within the nAChR extracellular

90 After proteolytic digestion of photolabeled DT-A, derivatized peptides were isolated us

91 er-free radioiodinated [125I]IAS was used to photolabel epitope-tagged human beta 2AR in membranes pr

92 Competition photolabeling established that this site binds with high

93 l-terminal fourth repeat of annexin from the photolabeling experiment using domain-deletion mutants o

94 ion kinetics, photoinactivation studies, and photolabeling experiments are also included; these exper

95 Photolabeling experiments have been particularly informa

96 Competitive radioligand binding and photolabeling experiments using well-characterized nonco

97 Photolabeling experiments with 8-azido-ATP demonstrate a

98 Photolabeling experiments with the McbA propeptide now i

99 Inhibition kinetics, photolabeling experiments, as well as X-ray crystallogra

100 -myeloid differentiation-2 (MD-2) complex by photolabeling experiments.

101 he lipid carbonyl carbons, in agreement with photolabeling experiments.

102 o the fusion proteins were obtained from the photolabeling experiments.

103 roteins, including BamA and LptD as shown by photolabeling experiments.

104 (d) approximately 10 nM) in both binding and photolabeling experiments.

105 nt stereospecific barbiturate anesthetic, to photolabel expressed human alpha1beta3gamma2 GABAARs.

106 Comparison of the photolabeled fragment from the subsequent proteolysis of

107 Photolabeled G proteins were either directly resolved us

108 [3H]APFBzcholine photolabeled gammaLeu-109/deltaLeu-111, gammaTyr-111, an

109 Azialcohols photolabel Glu-33 and Tyr-418, two residues that are pre

110 analogue photolabeling reagents in which the photolabeling groups were placed at three positions arou

111 rescent groups for the purpose of performing photolabeling have been prepared and evaluated using the

112 General anesthetic photolabels have been instrumental in discovering and co

113 3-Azioctanol photolabeled His-36, whereas its isomer, 7-azioctanol, p

114 nalogue, 16b, was radioiodinated and used to photolabel human DAT-transfected HEK 293 cell membranes.

115 binding sites in an extrasynaptic GABAAR, we photolabeled human alpha4beta3delta GABAARs purified in

116 IC50 = 40 mum) than it inhibited ion channel photolabeling (IC50 = 125 mum).

117 Photolabeling identified three distinct [(3)H]F(4)N(3)Bz

118 orms of RNase L has been completed utilizing photolabeling/immunoprecipitation and affinity assays, r

119 7, followed by thrombin digestion, retained photolabel in a 22-kDa fragment, indicating that iodoami

120 ace; the etomidate analog [(3)H]azietomidate photolabeled in a pharmacologically specific manner two

121 In addition, [(3)H]TFD-etomidate photolabeled in an agonist-dependent manner amino acids

122 (alphaGlu-390, alphaCys-412) that were also photolabeled in nAChRs in the equilibrium desensitized s

123 For nAChRs photolabeled in the absence of agonist (resting state),

124 ist and [(3)H]azietomidate, amino acids were photolabeled in the ion channel [position M2-20 (alphaGl

125 e alphaM1 and alphaM4 helices, identified by photolabeling in alphaM1 (alphaCys-222/alphaLeu-223); an

126 Comparison of nAChR photolabeling in the closed state (absence of agonist) a

127 The propofol-inhibitable [(3)H]AziPm photolabeling in the GABAAR beta3 subunit in conjunction

128 Photolabeling in the ion channel and alphaM1 was higher

129 domain: 1) in the ion channel, identified by photolabeling in the M2 helices of betaVal-261 and delta

130 unit site in the delta subunit helix bundle, photolabeling in the nAChR desensitized state (+agonist)

131 a site within the ion channel, identified by photolabeling in the nAChR desensitized state of amino a

132 and deltaCys-236); (ii) in the ion channel, photolabeling in the nAChR resting, closed channel state

133 -55 and deltaTrp-57, as the primary sites of photolabeling in the non-alpha subunits.

134 es elicited by the activation of efficacious photolabels in vivo with time-resolved proteomics provid

135 presence of agonist and the agonist-enhanced photolabeling inhibitable by phencyclidine.

136 tubulin by subtilisin after, but not before, photolabeling is blocked by probe 1.

137 The observed pattern of photolabeling is examined in relation to the predicted o

138 [(3)H]CPZ photolabeling is not detected in the transmembrane domai

139 or from detergent extracts of bovine cortex, photolabeled it with [(3)H]Ro15-4513, and identified (3)

140 The photolabeled kinase was subjected to tryptic digest, and

141 Photolabeling may help resolve this difficulty, and thus

142 ta)-6-azi-pregnanolone (6-AziP), was used to photolabel membranes from Sf9 cells expressing high-dens

143 Previous photolabeling, modeling, and functional data have identi

144 f [(3)H]physostigmine- or [(3)H]galanthamine-photolabeled nAChR establish that, in the presence of ag

145 f peptide fragments isolated from [(3)H]CMPI-photolabeled nAChR subunits established photolabeling of

146 idate, an intravenous general anesthetic, we photolabeled nicotinic acetylcholine receptor (nAChR)-ri

147 ng domains in a ligand-gated ion channel, we photolabeled nicotinic acetylcholine receptor (nAChR)-ri

148 n of this protein with CNBr/trypsin revealed photolabeling of a 2.9-kDa peptide.

149 imulated ABCB1 ATPase activity and inhibited photolabeling of ABCB1 with [(125)I]-iodoarylazidoprazos

150 erestingly, erlotinib slightly inhibited the photolabeling of ABCB1 with [(125)I]iodoarylazidoprazosi

151 imulated ABCG2 ATPase activity and inhibited photolabeling of ABCG2 with [(125)I]-IAAP.

152 ed as antihypertensive agents, inhibited the photolabeling of ABCG2 with [(125)I]IAAP and [(3)H]azido

153 gh concentration, but it did not inhibit the photolabeling of ABCG2 with IAAP.

154 at propofol inhibited to the same extent the photolabeling of alpha1Met-236 and betaMet-286.

155 However, it is unknown whether photolabeling of alphaE262 causes functional effects in

156 Irradiation at 254 nm resulted in photolabeling of alphaTyr(198) in agonist binding site S

157 CMPI-photolabeled nAChR subunits established photolabeling of amino acids contributing to the ACh bin

158 crosequencing, we found propofol-inhibitable photolabeling of amino acids in the beta3-alpha1 subunit

159 ing state), there was tetracaine-inhibitable photolabeling of amino acids in the ion channel at posit

160 ncing established 3alpha5alpha-P inhibitable photolabeling of amino acids near the cytoplasmic end of

161 The results of photolabeling of ArsA with the ATP analogue 8-azidoadeno

162 contrast, within the same site GABA enhances photolabeling of beta3Met-227 in betaM1 by an anesthetic

163 [(3)H]Azietomidate and [(3)H]R-mTFD-MPAB photolabeling of beta3Met-227 in betaM1 established that

164 re was also propofol-inhibitable [(3)H]AziPm photolabeling of beta3Met-227 in betaM1, the amino acid

165 [(3)H]azietomidate photolabeling of beta3Met-286 in betaM3 and alpha4Met-26

166 mical modification of this residue abolishes photolabeling of both channels with the ceramide probe.

167 05 in the vestibule of the ion channel, with photolabeling of both residues enhanced in the presence

168 fic, AMP-PCP-enhanced, [(3)H]azidodantrolene photolabeling of both the RyR monomer and a 160 or 172 k

169 Here, we use [(3)H]azietomidate photolabeling of bovine brain GABA(A)Rs to determine whe

170 imulated GLUT4 translocation, as assessed by photolabeling of cell surface GLUT4 with Bio-LC-ATB-BMPA

171 ition of agonist did not enhance [(125)I]TID photolabeling of deltaIle288 within the deltaM2-M3 loop.

172 Halothane photolabeling of deltaTyr-228 provides initial evidence

173 Within M1, the level of photolabeling of deltaTyr-228 with [(14)C]halothane was

174 [35S]GTPgammaS binding, a decrease in basal photolabeling of G-proteins with azidoanilido-[alpha-32P

175 We previously identified azietomidate photolabeling of GABA(A)R alpha1Met-236 and betaMet-286

176 (A)R-modulating neurosteroids do not inhibit photolabeling of GABA(A)R alpha1Met-236 or betaMet-286 b

177 Importantly, photolabeling of Galpha-subunits with azidoanilido-[alph

178 GABA inhibits S-[(3)H]mTFD-MPPB photolabeling of gamma2Ser-280 (gammaM2-15') in this sit

179 gamma-alpha subunit interface, identified by photolabeling of gammaMet299 within the gammaM3 helix at

180 Furthermore, [(3)H]physostigmine photolabeling of gammaTyr-111, gammaTyr-117, deltaTyr-21

181 ty labeling (BEProFL) approach that utilizes photolabeling of HDAC8 with a probe containing a UV-acti

182 al analyses, radioligand binding assays, and photolabeling of nAChR-rich membranes with [3H]BP to ide

183 Compound 28 inhibited the photolabeling of P-gp with [(125)I]-iodoarylazidoprazosi

184 P produced time- and concentration-dependent photolabeling of protein bands of approximately 35 and 6

185 [125I]IAmF photolabeling of recombinant VMAT2, expressed in SH-SY5Y

186 hyperspectral visual stimulation as well as photolabeling of RGCs to provide a functional and anatom

187 specifically inhibits [(3)H]azidodantrolene photolabeling of RyR1 and its N-terminal fragment in SR.

188 Saturation of photolabeling of the 80- and the 37-kDa RNase L with the

189 8-Azidoadenosine 5'-monophosphate photolabeling of the AMP-binding site and adenylate kina

190 Photolabeling of the beta3 subunits was stereoselective,

191 s not have an effect on the Kd value; and 3) photolabeling of the protein with a cysteine residue in

192 ([(125)I]TID) to compare the state-dependent photolabeling of the Torpedo nAChR before and after puri

193 The photolabeling of these amino acids suggests that when th

194 ersubunit sites, inhibited [(3)H]S-mTFD-MPPB photolabeling of these nAChR intrasubunit binding sites.

195 We now find that photolabeling of this pocket persists during the transit

196 Photolabeling of this protein by IAC was inhibited by SK

197 ein site, and propofol inhibited [(3)H]AziPm photolabeling of this site in myelin SIRT2.

198 solated from proteolytic digests established photolabeling of two residues: one within the alphaM1 tr

199 Preferential photolabeling on Pbeta from Pgamma position 40 and on Pa

200 xes, 8-azido-ATP was found to preferentially photolabel one chain of the homodimer, suggesting that t

201 To achieve photolabeling or photoligation of two substrates, one is

202 esthetic steroid alphaxalone, which enhanced photolabeling, or DS-2, a delta subunit-selective positi

203 assays of the ATPase activity of P-gp and by photolabeling P-gp with its transport substrate [(125)I]

204 ed His(8)-beta3 subunits identified a single photolabeled peptide, ALLEYAF-6-AziP, in the third trans

205 nity chromatography and reversed-phase HPLC, photolabeled peptides located within or near the phospha

206 lytic digestion and isolation of fluorescent photolabeled peptides.

207 This probe photolabels PfCRT in situ with high specificity.

208 oth agents were also effective and selective photolabels, photoincorporating into some, but not all,

209 The photolabeled PKCdelta C1B was subjected to tryptic diges

210 ytoplasmic end of the M2 ion channel domain, photolabeling positions M2-2, M2-6, and/or M2-9 in each

211 [(3)H]6-AziP photolabeled proteins of 30, 55, 110, and 150 kDa, in a

212 itate a small fraction of the 45- and 40-kDa photolabeled proteins, suggesting that these proteins as

213 igrates with either the 45-kDa or the 40-kDa photolabeled proteins.

214 termine the sites of cholesterol binding, we photolabeled purified mouse VDAC1 (mVDAC1) with photoact

215 beta (+)/alpha (-) pockets, the barbiturate photolabel R-5-allyl-1-methyl-5-(m-trifluoromethyl-diazi

216 Tracking of photolabeled Rac2-deficient neutrophils from hematopoiet

217 coupled to an alkyne-containing neurosteroid photolabeling reagent and used to identify peptide-stero

218 inding sites directly, a neurosteroid-analog photolabeling reagent, (3alpha,5beta)-6-azi-pregnanolone

219 4 was found to be an exceptionally efficient photolabeling reagent, incorporating into both alpha1 an

220 We synthesized neurosteroid analogue photolabeling reagents in which the photolabeling groups

221 alent attachment site for Bpa(4)-SP, a small photolabeled receptor fragment was generated by chemical

222 c and chemical fragmentation analysis of the photolabeled receptor mutants established that the sites

223 Fragmentation analysis of the photolabeled receptor restricted the site of photoincorp

224 ltaM2-13') that line the channel lumen (with photolabeling reduced by >90% in the desensitized state)

225 like the parent anesthetic, and identify two photolabeled residues (V954 and E969) in the S6 helix.

226 In the model, all of the photolabeled residues line the ligand binding cavity exc

227 ometry (MS), we identified three clusters of photolabeled residues representing three distinct neuros

228 le different adduct masses were found on the photolabeled residues, and the molecular identity of eac

229 8-azidoadenosine 5'-monophosphate (8-N3-AMP) photolabeled separate sites in CFTR.

230 Cyanogen bromide cleavage of the [125I]IACoc photolabeled sigma-1 receptor followed by radiosequencin

231 ore, after cleaving the specific [(125)I]IAF-photolabeled sigma-1 receptor in guinea pig and rat live

232 Cleavage of the photolabeled sigma-1 receptor using Endo Lys C and cyano

233 Instead, it photolabeled sites at the alpha(+)-beta(-) and gamma(+)-

234 table cholesterol analogues and analyzed the photolabeled sites with both top-down mass spectrometry

235 the feasibility of identifying neurosteroid photolabeling sites by using mass spectrometry.

236 tation of solubilized, [(3)H]azidodantrolene-photolabeled SR protein reveals that the cleaved 160/172

237 Using meta-azi-propofol (AziPm), we photolabeled stable 5-LOX protein, which had been used t

238 First, we use a photolabelling strategy to trace the connections that re

239 19-[3H]BPC-discodermolide), was selected for photolabeling studies because it had the highest extent

240 Photolabeling studies established that S-mTFD-MPPB binds

241 This was supported by photolabeling studies showing concentration- and UV-depe

242 Photolabeling studies using [3H]-3-azioctanol in Torpedo

243 Previous photolabeling studies with [(3)H]flunitrazepam identifie

244 DsRed-derived variants which we showcase in photolabeling studies, and discuss these data in terms o

245 Upon the basis of these photolabeling studies, we conclude that (1) subunits VII

246 rophenyl azido (pfpa) CQ analogues for PfCRT photolabeling studies.

247 he binding of ginkgolides to PAF receptor by photolabeling studies.

248 In this study, we developed an intact cell photolabeling technique that allows the direct visualiza

249 (D-mannose-4-yloxy)-2-propylamine exofacial photolabeling technique, was reduced by approximately 70

250 In the present study patch clamp and photolabelling techniques were used to investigate the m

251 The photolabeling technology developed here offers a new way

252 Tryptic peptides from the photolabeled terminase were purified by affinity chromat

253 tinic acetylcholine receptors (nAChRs) and a photolabel that incorporates both at the lipid-protein i

254 cing revealed that R-[(3)H]mTFD-MPAB did not photolabel the etomidate sites at the beta(+)-alpha(-) s

255 table analogs of 1-butanol and 1-octanol, to photolabel the purified Ig1-4 domain of human L1 (hL1 Ig

256 [(3)H]TFD-etomidate photolabeled the alpha-subunit of the nAChR in a manner

257 In living cells, BPyneTEA photolabels the closed state selectively over the inacti

258 icated that [(3)H]3'-(p-azidobenzamido)Taxol photolabels the N-terminal 31 amino acids of beta-tubuli

259 r of chiral barbiturates that are capable of photolabelling their binding sites on GABAA receptors.

260 was mutated to a glutamine, KK174 no longer photolabeled this residue, but instead labeled the nearb

261 AziPm photolabeled three SIRT2 residues (Tyr(139), Phe(190), a

262 Torpedo californica nAChRs and time-resolved photolabeling to identify the nAChR binding sites occupi

263 atidine in a muscle and a neuronal nAChR, we photolabeled Torpedo alpha(2)betagammadelta and expresse

264 -binding regions on Palpha and Pbeta through photolabel transfer from various Pgamma positions throug

265 were able to investigate this issue using a photolabel transfer strategy that allows for mapping the

266 candidate targets, we used a combination of photolabeling, two-dimensional gel electrophoresis, and

267 xing unit, a novel freeze-quench unit, and a photolabeling unit.

268 on the full ectodomain LFA-1 were probed by photolabeling using photoactivatable isoflurane (azi-iso

269 purified recombinant PfCRT, we analyze AzBCQ photolabeling versus competition with CQ and other drugs

270 Within alpha subunit, >/=95% of specific photolabeling was contained within a 20-kilodalton prote

271 Photolabeling was inhibited by anesthetic concentrations

272 [(32)P-5N(3)]NAADP photolabeling was irreversible in a high K(+) buffer, a

273 Photolabeling was performed after preincubation times of

274 e antagonist, or isoflurane, state-dependent photolabeling was seen in a delta subunit fragment begin

275 inhibitory effects elicited by these MSAs on photolabeling were distinct for beta-tubulin from differ

276 alphaM1 and deltaTyr-228 within deltaM1 were photolabeled, while no labeled amino acids were identifi

277 Purified UGT1A10 was photolabeled with 4-AzHBA, digested with trypsin, and an

278 in GDP-fucose transport and was specifically photolabeled with 8-azidoguanosine-5'-[alpha-(32)P]triph

279 ), opsin and membrane lipids were dominantly photolabeled with [(14)C]halothane, but none of the thre

280 nAChR-rich membranes were photolabeled with [(3)H]AziPm, and labeled amino acids w

281 subunits, isolated from nAChR-rich membranes photolabeled with [(3)H]Bz(2)choline, were digested enzy

282 nAChR-rich membranes were photolabeled with [(3)H]TDBzl-etomidate, and labeled ami

283 Subunits isolated from nAChR-rich membranes photolabeled with [(3)H]tetracaine were subjected to enz

284 -subunit, isolated from nAChR-rich membranes photolabeled with [3H]dTC, was digested with Staphylococ

285 ovary cells was specifically and efficiently photolabeled with a radioiodinated derivative of Bpa(4)-

286 a-tubulin isotype content, were specifically photolabeled with a tritium-labeled Taxol analog, 2-(m-a

287 Myofibrils photolabeled with AziPm and Azi-iso identified myosin, a

288 ylcholine vesicles and could be specifically photolabeled with P3-(4-azidoanilido)-uridine-5'-[P1-32P

289 To identify this site, PKCdelta C1B was photolabeled with three photo-activable diazirine alcoho

290 vesicles of defined composition and by using photolabeling with 3-trifluoromethyl-3-(m-[125I]iodophen

291 Conversely, AMP enhanced photolabeling with 8-N3-ATP at ATP-binding site 2.

292 We now use competition photolabeling with [(3)H]azietomidate and [(3)H]R-mTFD-M

293 shows greatly decreased activity, abolished photolabeling with [32P]8N3ATP, and no detectable autoin

294 ytochrome c oxidase (CcO) were identified by photolabeling with arylazido-cardiolipin analogues and d

295 rs that combines attributes of high-contrast photolabeling with high-sensitivity Ca(2+) detection in

296 ain of the Torpedo nAChR using time-resolved photolabeling with the hydrophobic probe 3-(trifluoromet

297 and cell surface GLUT4 levels as assessed by photolabeling with the membrane-impermeant reagent 2-N-(

298 hetic sites on GABAA receptors (GABAA Rs) by photolabelling with an anaesthetic barbiturate.

299 Propofol inhibited [(3)H]AziPm photolabeling within the delta subunit helix bundle at l

300 lower-efficiency, state-dependent [(3)H]CMPI photolabeling within the ion channel.

301 or the purified nAChR, the agonist-sensitive photolabeling within the M2 ion channel domain of positi