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1 the center of the cell in lieu of a midbody/phragmoplast.
2 N cell plate that is formed within the solid phragmoplast.
3 , transitional phragmoplast, and ring-shaped phragmoplast.
4 e preprophase band, mitotic spindle, and the phragmoplast.
5 e transport of Golgi-derived vesicles in the phragmoplast.
6 al signal could be detected elsewhere in the phragmoplast.
7 reorganization and vesicle transport in the phragmoplast.
8 trated on MTs in the spindle midzone and the phragmoplast.
9 although some mitochondria can approach the phragmoplast.
10 reprophase band, the mitotic spindle and the phragmoplast.
11 to the midzone of the mitotic apparatus and phragmoplast.
12 rmation of a cell plate in the center of the phragmoplast.
13 ortex, preprophase band, mitotic spindle and phragmoplast.
14 letal assemblies that received the expanding phragmoplast.
15 otubule and a microfilament array called the phragmoplast.
16 tion of MAP65-3 with microtubules within the phragmoplast.
17 ed localization in the midzone of developing phragmoplast.
18 ortex, preprophase band, mitotic spindle and phragmoplast.
19 terials around the circumferentially growing phragmoplast.
20 nd during cytokinesis is associated with the phragmoplast.
21 ow that TIO is required for expansion of the phragmoplast.
22 of gamma-tubulin in the mitotic spindle and phragmoplast.
23 tiparallel MTs toward their plus ends in the phragmoplast.
24 ntiparallel MTs to be closely engaged in the phragmoplast.
25 dynamic cytoskeletal apparatus known as the phragmoplast.
26 lus ends of antiparallel microtubules in the phragmoplast.
27 t cells, cytokinesis is brought about by the phragmoplast.
28 lel microtubules in the middle region of the phragmoplast.
29 ials and their CPAMs gives rise to the solid phragmoplast.
30 and regions rich in growing plus-ends within phragmoplasts.
36 gaging and bundling anti-parallel MTs in the phragmoplast and disclosed a novel action of MAP65-4 at
37 ng that short-range interactions between the phragmoplast and plasma membrane may play important role
38 t that microtubules initiate randomly in the phragmoplast and that the majority exhibit dynamic insta
39 tiva) microtubule-associated proteins in the phragmoplast and uncovered a novel spindle-associated mi
42 rce of MT generation for assembling spindle, phragmoplast, and cortical arrays when the gamma-tubulin
43 3 was localized along MTs in the spindle and phragmoplast, and its signal was pronounced in anaphase
46 TEN, formin also localized to the cell apex, phragmoplast, and to the cell cortex as dynamic cortical
47 that microtubule nucleation factors regulate phragmoplast architecture and axial asymmetry directly b
48 imaging reveals that the mitotic spindle and phragmoplast are laterally displaced, and that the growi
50 riety of observations suggest that expanding phragmoplasts are actively guided to the former PPB site
53 ymmetric preprophase bands prior to mitosis; phragmoplasts are subsequently guided to these asymmetri
56 phragmoplast-specific motors Kinesin-12 and Phragmoplast-Associated Kinesin-Related Protein2 to inte
58 clusive model where microtubule dynamics and phragmoplast asymmetry are consistent with the localizat
59 al information to be read by the cytokinetic phragmoplast at later stages of mitotic cell division.
63 nto phragmoplast-like structures termed mini-phragmoplasts between both sister and nonsister nuclei.
69 formed cross walls at the stage in which the phragmoplast cytoskeleton has depolymerized and the new
70 then remained near the spindle midzone until phragmoplast development, at which time they were again
71 bule and actin filament structure called the phragmoplast directs vesicles to create the new cell wal
74 of cell division depends on guidance of the phragmoplast during cytokinesis to a cortical site marke
76 endent process necessary for the guidance of phragmoplasts during cytokinesis in asymmetrically divid
78 rotubules (MTs) in the cytokinetic apparatus phragmoplast exhibit an antiparallel array and transport
79 nt cytokinetic microtubule array, called the phragmoplast, exhibits higher microtubule dynamics in it
82 oplast guidance in o1 is normal; however, as phragmoplast expansion continues o1 phragmoplasts become
84 ation of a Fused kinase signalling module in phragmoplast expansion that depends upon conserved struc
85 together with actin plays a role in guiding phragmoplast expansion to the cortical division site.
86 t myosins are important at multiple steps of phragmoplast expansion, and the O1 actin motor and POK-l
90 keletal arrays, the preprophase band and the phragmoplast, facilitate the positioning and de novo ass
91 okinesis, the microtubule- and F-actin-based phragmoplast facilitates construction of a new cell wall
92 t on anaphase transition but causes aberrant phragmoplast formation and delays the completion of cyto
95 Following nuclear division, a cytokinetic phragmoplast forms between the daughter nuclei and expan
98 hase band formation did not prevent accurate phragmoplast fusion, and subsequent cell plate formation
99 he same alanine substitutions had defects in phragmoplast guidance and reduced TAN1 and POK1 localiza
102 This regulatory function of MYA1/Myo11F in phragmoplast guidance was dependent on intact actin fila
108 example the FtsZ ring in bacteria [15], the phragmoplast in plants [16], and the actomyosin ring in
110 centrosome, organization of the spindles and phragmoplasts in mitosis is known to involve the evoluti
111 the same time, the lateral expansion of the phragmoplast initials and their CPAMs gives rise to the
112 (ER) membranes are seen associated with the phragmoplast initials and with the TVN cell plate that i
114 his process can be divided into four phases: phragmoplast initials, solid phragmoplast, transitional
115 tbooks is of a symmetrical process, with the phragmoplast initiating in the center of the cell and gr
117 out this process, the advancing front of the phragmoplast is in intimate contact with the parental wa
118 rows the microtubule nucleation angle at the phragmoplast leading edge and functions as a microtubule
119 microtubule nucleation angle, but increases phragmoplast length and slows down phragmoplast expansio
120 errations in the spatial organization of the phragmoplast-like radial microtubule arrays (RMAs) at th
121 roximately 10 microtubules in each set) into phragmoplast-like structures termed mini-phragmoplasts b
122 reviously not recognized ectopic spindle- or phragmoplast-like structures that arise without attachme
123 Despite being a major characteristic of the phragmoplast, little is known about regulators of this p
124 inases indicated as ARABIDOPSIS NUCLEUS- AND PHRAGMOPLAST-LOCALIZED KINASE1-RELATED PROTEIN KINASE1 (
125 Plant cells assemble the bipolar spindle and phragmoplast microtubule (MT) arrays in the absence of t
130 are incorporated into cortical, spindle and phragmoplast microtubule arrays indicating that they are
131 (AtAurora1), spindle microtubules (AtEDE1), phragmoplast microtubules (AtMAP65-4) and the cell plate
133 that myosin VIII associates with the ends of phragmoplast microtubules and together with actin plays
134 play a critical role in the organization of phragmoplast microtubules during cytokinesis in the micr
136 ta lead to a model whereby myosin VIII links phragmoplast microtubules to the cortical division site
137 beling experiments demonstrated that, unlike phragmoplast microtubules which are concentrated on the
139 cells anti-DcKRP120-2 strongly decorates the phragmoplast mid-line where the plus ends of the microtu
143 , KINESIN-12E shares its localization at the phragmoplast midzone with several functionally diversifi
144 a-Aurora relocated from spindle poles to the phragmoplast midzone, where it interacted with the micro
148 d variable spindle orientations and enhanced phragmoplast mobility, suggesting that the PPB is involv
150 ge at unified spindle poles, and the bipolar phragmoplast MT array frequently had discrete bundles wi
151 role in establishing and/or maintaining the phragmoplast MT array, and AtPAKRP2 may contribute to th
153 augmin and gamma-tubulin on the spindle and phragmoplast MT arrays and leads to serious distortions
154 is associated with acentrosomal spindle and phragmoplast MT arrays in patterns indistinguishable fro
156 tion, defective cells exhibited disorganized phragmoplast MT arrays, which caused aborted cytokinesis
157 lope breakdown, they localize on spindle and phragmoplast MTs and on the reforming nuclear envelope o
158 scopy, we observed that certain antiparallel phragmoplast MTs overlapped and were bridged by electron
162 transport along microtubules of the bipolar phragmoplast network that guides plate assembly [7-9].
163 imal cells is functionally equivalent to the phragmoplast of plants and acts to target secretion to t
166 genes in Arabidopsis thaliana (A. thaliana): PHRAGMOPLAST ORIENTING KINESIN 1 and 2 (POK1, POK2).
168 ow that the division site-localized protein, PHRAGMOPLAST ORIENTING KINESIN1 (POK1), was aberrantly l
169 e Arabidopsis thaliana division site markers PHRAGMOPLAST ORIENTING KINESINs (POKs), which are also r
170 he domain of overlapping microtubules at the phragmoplast perimeter, limiting the site of material de
172 cal microtubules during telophase to mediate phragmoplast positioning at the final division plane.
176 rst phase is confined to the cylinder of the phragmoplast proper and is followed by a second phase th
180 ion, rapid microtubule reorganization in the phragmoplast requires the orchestrated activities of mic
181 icle trafficking, they were required for the phragmoplast-specific motors Kinesin-12 and Phragmoplast
183 continues to label the midline of the early phragmoplast, suggesting a structural continuity with th
184 PB is disassembled and directs the expanding phragmoplast to the former PPB site during cytokinesis.
186 n guidance of the cytokinetic apparatus, the phragmoplast, to a cortical "division site" established
187 a spatial reminder that actively guides the phragmoplast towards the cortical division site during c
188 to four phases: phragmoplast initials, solid phragmoplast, transitional phragmoplast, and ring-shaped
189 analyzed the behavior of microtubules in the phragmoplast using live-cell imaging coupled with mathem
190 is followed by a second phase that deposits phragmoplast vesicles in a concentric fashion, resulting
192 associated with the mitotic spindle, and the phragmoplast was depleted when GCP4 was downregulated.
193 antiparallel MTs in the central spindle and phragmoplast was largely abolished in mutant cells lacki
195 poral and spatial organization of PDL in the phragmoplast, we termed this protein 'phragmoplastin'.
196 rotubules at the leading edge of the matured phragmoplast were often disengaged, accompanied by consp
198 Plant cytokinesis is brought about by the phragmoplast, which contains an antiparallel microtubule
199 nd (PPB) and the subsequent expansion of the phragmoplast, which deposits the new cell wall, to the c
200 depends on a cytoskeletal structure called a phragmoplast, which directs the formation of a new cell
201 lves a microtubule-containing structure, the phragmoplast, which guides the formation of new cell wal
202 zation of growing microtubules ends from the phragmoplast with TAN1 at the division site suggests tha