ライフサイエンスコーパス: phytoene

1 he sole product of the reaction was 15,15'-Z-phytoene.

2 t of the cyclopropylcarbinyl intermediate to phytoene.

3 in (GFP), did not photo-bleach or accumulate phytoene.

4 sectors accumulate the carotenoid precursor phytoene.

5 eranylgeranyl diphosphate, zeta-carotene, or phytoene.

6 ed: dehydrosqualene (DSQ), a C30 analogue of phytoene; 10(S)-hydroxysqualene (HSQ), a hydroxy analogu

7 zeaxanthin (1.28), beta-cryptoxanthin (2.8), phytoene (18.68) and phytofluene (7.45) in a CE using DE

8 and responsible for the synthesis of 15-cis-phytoene, 9,9'-di-cis-zeta-carotene, and all-trans-lycop

9 Other phytoene-accumulating mutant endosperms, vp2 and white3

10 stigate this regulation further, we examined phytoene-accumulating tissue in Arabidopsis thaliana (L.

11 Previous analyses of phytoene-accumulating tissue suggested that there may be

12 Two types of phytoene-accumulating tissue were studied: Norflurazon-b

13 PYGG converted GGPP to phytoene almost quantitatively in vitro and did not show

14 (15Z)-Phytoene and (all-E)-lutein showed the greatest bioacces

15 The CPSFL1 protein bound phytoene and beta-carotene when expressed in Escherichia

16 is that C40 carotenoids are synthesized from phytoene and C30 carotenoids from diapophytoene.

17 A strong correlation was noted between phytoene and carotenoid components in this study (capsan

18 restored to a level sufficient for producing phytoene and downstream carotenoids.

19 ch green microalgal extracts along with pure phytoene and lutein, on the epidermis of the nematode Ca

20 Others were zeta-carotene, phytofluene, phytoene and lutein.

21 Phytoene and phytofluene accumulation tended to be more

22 Phytoene and phytofluene are major abundant dietary caro

23 Phytoene and phytofluene are precursor molecules of high

24 ) treatment resulted in high accumulation of phytoene and phytofluene in both oranges, and the biosyn

25 The bioaccessibility of phytoene and phytofluene in tomato, carrot, blood orange

26 decreased with increased heating times while phytoene and phytofluene were unchanged.

27 y with a significant reduction in carotenes (phytoene and phytofluene) in the PG fraction.

28 The bioaccessibility of phytoene and phytofluene, and also total carotenoid bioa

29 products, on carotenoid contents, including phytoene and phytofluene, as well as vitamin E.

30 The pds(-) and zds(-) strains synthesize phytoene and zeta-carotene, respectively, both of which

31 the contents of vitamin C, alpha-tocopherol, phytoene, and beta-carotene in fruits; however, the effe

32 Plastoglobuli contain beta-carotene, phytoene, and galactolipids missing in CLDs.

33 after treatment were compared for lycopene, phytoene, and phytofluene contents.

34 This study evaluates the effect of phytoene- and lutein-rich green microalgal extracts alon

35 Efficient phytoene biosynthesis via PSY strictly depended on simul

36 To optimize phytoene biosynthesis, we applied a synthetic biology ap

37 did not cleave geranylgeranyl diphosphate or phytoene but did cleave other linear and cyclic caroteno

38 In a second stage, phytoene conversion into downstream carotenoids is requi

39 , Mg-protoporphyrin IX chelatase (bchD), and phytoene dehydrogenase (crtI) demonstrate RegA is respon

40 of the operon and the carC gene that encodes phytoene dehydrogenase.

41 hange the product of Rhodobacter sphaeroides phytoene desaturase (crtI gene product), a neurosporene-

42 Replacing the native 3-step phytoene desaturase (CrtI) with the 4-step enzyme from E

43 identified: phytoene synthase (crtB/CT1386), phytoene desaturase (crtP/CT0807), zeta-carotene desatur

44 chocystis sp. PCC 6803 the genes that encode phytoene desaturase (encoded by crtP (pds)) and zeta-car

45 ions of this FoMV vector system, four genes, phytoene desaturase (functions in carotenoid biosynthesi

46 ces is effective at silencing the endogenous phytoene desaturase (PapsPDS) gene in Papaver somniferum

47 mate-1-semialdehyde aminotransferase (GSAT), phytoene desaturase (PDS) and light-harvesting polypepti

48 Infection with TRV containing a phytoene desaturase (PDS) fragment resulted in reduced a

49 at express a fragment of the nuclear-encoded Phytoene desaturase (PDS) gene capable of catalyzing pos

50 We have successfully silenced the phytoene desaturase (PDS) gene in the diploid wild speci

51 gineered with magnesium chelatase (ChlH) and phytoene desaturase (PDS) gene sequences from Nicotiana

52 cylic acid differed statistically in normal, phytoene desaturase (PDS) gene silent and diseased (infe

53 ent protein (gfp) transgene or an endogenous phytoene desaturase (pds) gene, generated a stronger and

54 ences from the RNA leader of the Arabidopsis phytoene desaturase (pds) gene, when inserted into the 3

55 e novo shoots with knockout mutations of the phytoene desaturase (PDS) gene.

56 ne desaturase gene (zds) or both the zds and phytoene desaturase (pds) genes of Synechocystis sp. PCC

57 Phytoene desaturase (PDS) is required for synthesizing c

58 nalyzed 20 progeny plants of Cas12a-mediated phytoene desaturase (PDS) mutagenized regenerants, as we

59 nd expressing the VIGS constructs to silence Phytoene desaturase (PDS) or a ribosomal protein-encodin

60 tenoid biosynthetic pathway, a cDNA encoding phytoene desaturase (PDS) was isolated and characterized

61 h we successfully silenced the expression of phytoene desaturase (PDS), a 20S proteasome subunit (PB7

62 to zeta-carotene, carried out by the enzyme phytoene desaturase (PDS), is one of the earliest steps

63 pressing transgenic sorghum lines, targeting Phytoene desaturase (PDS), Magnesium-chelatase subunit I

64 TRV with gRNA targeted to Phytoene desaturase (SlPDS) and Downy mildew resistance

65 by phytoene synthase (PSY), two desaturases (phytoene desaturase [PDS] and zeta-carotene desaturase [

66 lack colored carotenoids due to a defect in phytoene desaturase activity.

67 stewartii, and the two carotene desaturases phytoene desaturase and carotene zeta-carotene desaturas

68 ded a vector, BMVCP5, that better maintained phytoene desaturase and heat shock protein70-1 (HSP70-1)

69 block the carotenoid pathway at the level of phytoene desaturase and induce the accumulation of phyto

70 arrying geranylgeranyl diphosphate synthase, phytoene desaturase and the bacterial carotene desaturas

71 to target an endogenous transcript encoding PHYTOENE DESATURASE and used to analyze the role of miR1

72 ownstream enzymes, required two desaturases (phytoene desaturase and zeta-carotene desaturase [ZDS])

73 As the phytoene desaturase and zeta-carotene desaturase used or

74 share significant similarity and a putative phytoene desaturase domain with a recently described pla

75 tem was first optimized in studies silencing phytoene desaturase expression.

76 genes of Synechocystis sp. PCC 6803 with the phytoene desaturase gene (crtI) of Rhodobacter capsulatu

77 esium chelatase subunit I gene (ChlI) or the phytoene desaturase gene (PDS).

78 The phytoene desaturase gene was silenced with a transient h

79 mutant capable of inducing silencing of the PHYTOENE DESATURASE gene.

80 ith a control construct or one that silences phytoene desaturase had no effect on resistance or susce

81 ude that the differences in the mechanism of phytoene desaturase inhibition play an important role in

82 hereas in the dark mainly zeta-carotene, the phytoene desaturase product, accumulates, illumination l

83 f green fluorescent protein and silencing of phytoene desaturase shows that marker gene-assisted sile

84 -PCR of intact RNA showed that the amount of phytoene desaturase transcripts increased after HHP trea

85 s, and the biosynthetic activity upstream of phytoene desaturase was similar in Newhall and Cara Cara

86 ts contained an insertion in a gene encoding phytoene desaturase, an enzyme of carotenoid biosynthesi

87 ts are rescued by inhibitors or mutations of phytoene desaturase, demonstrating that phytofluene and/

88 ,15,9'-tri-cis-zeta-carotene, the product of phytoene desaturase, to form 9,9'-di-cis-zeta-carotene,

89 l carotenoid gene (crtI) encoding the enzyme phytoene desaturase, which converts phytoene into lycope

90 ne in the sequence of reactions catalyzed by phytoene desaturase.

91 siRNAs targeting an endogenous mRNA encoding PHYTOENE DESATURASE3 was introduced into a protein-codin

92 ) A and B, as well as to bacterial and plant phytoene desaturases (PHD).

93 olutionarily preserved activity of bacterial phytoene desaturases and plant carotenoid isomerases.

94 We expressed shuffled phytoene desaturases in the context of a carotenoid bios

95 Phytoene desaturases occurring in nature convert phytoen

96 Approximately 10% of known phytoene desaturases, as in Rhodobacter, produce neurosp

97 a component of a redox chain responsible for phytoene desaturation but that a redundant electron tran

98 Both GGPP formation and phytoene desaturation were elevated in these mutants.

99 ne, IM likely serves as a redox component in phytoene desaturation.

100 CrtI, which mediates lycopene formation from phytoene, does not require light, nor is a poly-cis-lyco

101 essibility of carotenoids from wild-type and phytoene-enriched Dunaliella bardawil biomass treated by

102 Results revealed that phytoene-enriched microalgae at 3 mug/mL significantly i

103 The profile of phytoene formation during ripening was also different in

104 Phytoene formation mediated by phytoene synthase (PSY) i

105 of im accumulate the noncolored carotenoid, phytoene, IM likely serves as a redox component in phyto

106 ed in 'Tigerella' and 'Byelsa', and those of phytoene in 'Orange', those of phenolics in 'Green Zebra

107 ne desaturase and induce the accumulation of phytoene in C. sorokiniana.

108 tudy aimed at optimizing the accumulation of phytoene in Chlorella sorokiniana by using norflurazon a

109 carotenoids and carotenoid precursors beyond phytoene in dark-grown mutant cells.

110 f carotenoids in ripe fruit the formation of phytoene in vitro was detected in fruit of both mutants.

111 at in cyanobacteria and plants by converting phytoene into lycopene using two plant-like desaturases

112 e enzyme phytoene desaturase, which converts phytoene into lycopene.

113 onstituents such as carotenoids (capsanthin, phytoene, lutein, beta-cryptoxanthin), polyphenols conte

114 mulation of malondialdehyde and carotenoids (phytoene, lutein, lycopene, and beta-carotene) was delay

115 and 0.08 +/- 0.01 mumol/L.h for phytofluene, phytoene, lycopene and beta-carotene, respectively).

116 ue to high accumulation of carotenes, mainly phytoene, lycopene and phytofluene.

117 Phytoene overproduction initially interferes with photos

118 ical isomers of major carotenoids in humans (phytoene, phytofluene, lutein, zeaxanthin, beta-cryptoxa

119 The mechanisms of main tomato carotenes (phytoene, phytofluene, lycopene and beta-carotene) intes

120 ces were in carotenoids, including lycopene, phytoene, phytofluene, neurosporene, and zeta-carotene.

121 Phytoene, phytofluene, zeta-carotene, neurosporene, tetr

122 ed highest amounts of potentially absorbable phytoene/phytofluene was by far tomato juice (5mg/250mL

123 Phytoene-rich first described C. sorokiniana biomass was

124 the following six enzymes to be identified: phytoene synthase (crtB/CT1386), phytoene desaturase (cr

125 Phytoene synthase (PSase) catalyzes the condensation of

126 e 2 (CCD2) promoter while CstMYB1R2 binds to phytoene synthase (PSY) and CCD2 promoters.

127 ined, or sequence or abundance of mRNAs from phytoene synthase (PSY) and chromoplast-specific lycopen

128 Phytoene synthase (PSY) catalyzes the highly regulated s

129 Phytoene synthase (PSY) catalyzes the highly regulated,

130 orophyll biosynthesis pathway in addition to phytoene synthase (PSY) in carotenoid biosynthesis pathw

131 Phytoene formation mediated by phytoene synthase (PSY) is rate limiting in the biosynth

132 Phytoene synthase (PSY) is the crucial plastidial enzyme

133 Phytoene synthase (PSY) mediates the first committed ste

134 ltered splicing and C-terminal truncation of phytoene synthase (PSY), a key enzyme in carotenoid bios

135 Phytoene synthase (PSY), a major rate-controlling carote

136 limiting enzyme for carotenoid biosynthesis, phytoene synthase (PSY), as compared with white-rooted c

137 hypothesized that the daffodil gene encoding phytoene synthase (psy), one of the two genes used to de

138 ent, we began to characterize genes encoding phytoene synthase (PSY), since this nuclear-encoded enzy

139 control the expression of the gene encoding PHYTOENE SYNTHASE (PSY), the first and main rate-determi

140 he plastid-localized GGPS isoform GGPS11 and phytoene synthase (PSY), the first enzyme of the caroten

141 eract with GBFs to activate transcription of phytoene synthase (PSY), the gene encoding a rate-limiti

142 Phytoene synthase (PSY), the rate-limiting enzyme in the

143 ceed through a poly-cis pathway catalyzed by phytoene synthase (PSY), two desaturases (phytoene desat

144 d levels by posttranscriptionally regulating phytoene synthase (PSY).

145 c pathway is mediated by the nuclear-encoded phytoene synthase (PSY).

146 Expression analysis of PHYTOENE SYNTHASE (PSY1) and CAROTENOID ISOMERASE (CRTIS

147 isrupting the activity of the fruit-specific phytoene synthase (PSY1), the first committed step in th

148 genome editing we validated the function of PHYTOENE SYNTHASE 1 (PSY1) gene in affecting millet grai

149 SlBBX20 can activate the expression of PHYTOENE SYNTHASE 1, encoding a key enzyme in carotenoid

150 hosphate (IPP) was used as the substrate for phytoene synthase a reduction (e.g. r,r mutant, 5-fold)

151 Contrastingly, reduced phytoene synthase activity was not detected when [3H]GGP

152 rotenoid content, on two maize genes, the Y1 phytoene synthase and PSY2, a putative second phytoene s

153 Upon induction, recombinant phytoene synthase constituted 5-10% of total soluble pro

154 DNA sequencing of the phytoene synthase gene from each of the mutants revealed

155 e resulting from the up-regulation of the Y1 phytoene synthase gene product in endosperm tissue.

156 Transformation with a wild-type copy of the phytoene synthase gene was able to complement the lts1-2

157 as closely linked to a marker located in the phytoene synthase gene.

158 ts examined in this work are affected in the phytoene synthase gene.

159 Our findings underscore the diverse roles of phytoene synthase in shaping horticultural traits, and r

160 hese data were supported by the detection of phytoene synthase protein on western blots.

161 matoes is due to a mutated or down-regulated phytoene synthase protein, respectively, resulting in th

162 The crtB gene encoding phytoene synthase was isolated from a plasmid containing

163 d the expression of HMG2, PSY1 (the gene for phytoene synthase), and lycopene accumulation before the

164 ruit color accumulation (bifunctional 15-cis-phytoene synthase, 9-cis-epoxycarotenoid dioxygenase, be

165 s, as well as with the enhanced abundance of phytoene synthase, a key enzyme in the carotenoid biosyn

166 The phytoene synthase, and carotene desaturase proteins did

167 ymes geranylgeranyl diphosphate synthase and phytoene synthase, from the soil bacterium Erwinia stewa

168 Notably, phytoene synthase, involved in carotene biosynthesis, wa

169 The FN68 mutant is deficient in phytoene synthase, the first enzyme of the carotenoid bi

170 te synthase rather than squalene synthase or phytoene synthase, which catalyze c1'-2-3 cyclopropanati

171 es were concurrently upregulated, except for phytoene synthase, which was repressed.

172 anisms, acting predominantly at the level of phytoene synthase-1 (PSY1), and feed-forward mechanisms

173 fruit phenotype of the r,r mutant and Psy-1 (phytoene synthase-1) antisense tomatoes is due to a muta

174 e acting upon diketopiperazine substrates, a phytoene synthase-like prenyltransferase as the catalyst

175 hytoene synthase and PSY2, a putative second phytoene synthase.

176 s and Myxococcus xanthus crtB genes encoding phytoene synthase.

177 s of maize was sequenced and found to encode phytoene synthase.

178 and beta-glucosidase (beta-Glu), as well as phytoene synthase1 (PSY1) involved in carotenoid synthes

179 but functionally diverged from, the squalene/phytoene synthases family.

180 n bacteria and bifunctional lycopene cyclase-phytoene synthases in fungi.

181 that CPSFL1 is involved in the regulation of phytoene synthesis and carotenoid transport and thereby

182 embrane-bound portion mediated a doubling of phytoene synthesis rates.

183 gments became photo-bleached and accumulated phytoene (the substrate for PDS) in a manner similar to

184 rom impaired biosynthesis at a step prior to phytoene, the committed precursor to carotenoids.

185 ically inducing a burst in the production of phytoene, the first committed intermediate of the carote

186 Moreover, phytoene, the precursor of the pathway, was identified i

187 oene desaturases occurring in nature convert phytoene to either neurosporene or lycopene in most euba

188 sport chain and convert the colorless 15-cis-phytoene to the red-colored all-trans-lycopene.

189 The two-step desaturation of phytoene to zeta-carotene, carried out by the enzyme phy

190 o catalyze two desaturation steps converting phytoene to zeta-carotene.

191 rsion of the C40 carotenoid backbone, 15-cis-phytoene, to all-trans-lycopene, the geometrical isomer

192 duced six rather than four double bonds into phytoene, to favor production of the fully conjugated ca

193 Phytoene was consistently the carotenoid with the highes

194 e.g. r,r mutant, 5-fold) in the formation of phytoene was observed with an accumulation (e.g. r,r mut

195 Plasma concentrations of phytofluene and phytoene, which were present in small amounts in tomato