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1 cluding the natural ketolides narbomycin and pikromycin.
3 including methymycin (2), neomethymycin (3), pikromycin (4), and narbomycin (5) produced by Streptomy
7 10-deoxymethynolide, whereas narbomycin and pikromycin are derived from the 14-membered ring macrola
8 e Streptomyces venezuelae pikD gene from the pikromycin biosynthetic cluster was analyzed, and its de
12 s methymycin, neomethymycin, narbomycin, and pikromycin in Streptomyces venezuelae, is described.
14 Assembly line engineering is performed using pikromycin modules, with synthases designed using the up
15 roduction of the final glycosylated products pikromycin, narbomycin, methymycin and neomethymycin) an
16 nomodular type I polyketide synthases of the pikromycin pathway in vitro followed by direct appendage
17 eactivity of a monooxygenase (PikC) from the pikromycin pathway is modified through computationally g
18 tecture of a full-length PKS module from the pikromycin pathway of Streptomyces venezuelae creates a
20 synthesized and assayed a series of modified pikromycin (Pik) pentaketides that mimic early pathway e
21 e 6-deoxyerythronolide B synthase (DEBS) and pikromycin (Pik) polyketide synthase (PKS) are unique mu
24 eneck processing unnatural substrates in the pikromycin (Pik) system, preventing the formation of epi
27 cryo-microscopy structures of a full-length pikromycin PKS module in three key biochemical states of
28 preferences of the final two modules of the pikromycin PKS were compared for several non-natural ext
31 ample in which alternative expression of the pikromycin polyketide synthase results in the generation
32 isolated from a PKS-like gene cluster of the pikromycin producer Streptomyces venezuelae ATCC 15439,
35 s erythromycin and oleandomycin, each of the pikromycin series producers harbors a six module PKS clu
36 the C2-type KR from the third module of the pikromycin synthase, analogous to the KR from the third