ライフサイエンスコーパス: pipet

1 pulling platinum (Pt) wires in a theta glass pipet.

2 vity enclosed in the terminal of a capillary pipet.

3 rofluidic structures by using a conventional pipet.

4 of a laser-pulled quartz quadruple-barrelled pipet.

5 bout 3 times the inner opening radius of the pipet.

6 to that of a manually spotted volume with a pipet.

7 and the complex in the outer solution of the pipets.

8 gement is used for characterization of theta-pipets.

9 e configurations of traditional multichannel pipets.

10 with 200 muL of 0.9% NaCl buffer directly by pipetting.

11 immediately dispersed into single cells upon pipetting.

12 and compared its effectiveness with regular pipetting.

13 n turnaround time and cost, with less manual pipetting.

14 with reagent liquids, or lab skills such as pipetting.

15 samples and reagents are moved in and out by pipetting.

16 g SLT-1B from complex cell lysates simply by pipetting 20 muL of the sample in and out of the tip in

17 elative standard deviation similar to manual pipetting (3.0% vs 1.4%).

18 hite powder or nanotubes in acetonitrile and pipeting a small volume onto the electrode surface and a

19 enotypes of E. coli can be assayed by merely pipetting a cell suspension into seven microplate arrays

20 atforms are slow, and they typically rely on pipetting a fixed volume into the assay cards.

21 , inner pipet) and aspirate (the sink, outer pipet) a buffer solution (perfusate) into each of the tw

22 ferent substrates such as the tip of a glass pipet, a double-barrel pipet, and a freestanding silicon

23 s down to approximately 100 nL, although the pipetting accuracy and precision deteriorate considerabl

24 bes comparative results between the use of a pipet and a microfluidic-based sampling device for the c

25 g inocula of 10(5) or 10(6) CFU dispensed by pipet and by use of a cotton swab dipped in a 0.5 McFarl

26 of a laser-pulled quartz quadruple-barreled pipet and filling the open channels with electrolyte sol

27 without discontinuous steps in an automatic pipet and integrated seamlessly with glycan release and

28 ng to the ingress of the common ion into the pipet and its egress into the external solution.

29 s, which are formulated by approximating the pipet and meniscus using 1-D spherical coordinates.

30 Analytical data from DBS prepared with a pipet and microfluidic-based sampling devices were compa

31 istive pulse (RP) measurements in a nanopore pipet and vesicle impact electrochemical cytometry (VIEC

32 thrombin clots that are capable of plugging pipets and automated liquid-transfer systems.

33 table clamp, so on-chip operation only needs pipetting and adjusting of clamping force.

34 protocols are presented, one that minimizes pipetting and another that minimizes the number of react

35 Further, errors in repetitive pipetting and calculation are eliminated.

36 ple microwells thus avoiding repeated manual pipetting and costly robots.

37 ys may be performed with standard laboratory pipetting and detection systems.

38 Pipetting and dilution are universal processes used in c

39 ocular-surface epithelia can be isolated by pipetting and FACS sorting into a population of corneal

40 other biomolecules in a solution with manual pipetting and has many drawbacks such as being labor-int

41 rning bioreagents into microarrays by simply pipetting and incubating bioreagent solutions in compart

42 ability to dispense (i.e., the source, inner pipet) and aspirate (the sink, outer pipet) a buffer sol

43 as the tip of a glass pipet, a double-barrel pipet, and a freestanding silicon nitride membrane.

44 using a transfer pipet, docked to a holding pipet, and held firmly during physical agitation and int

45 re individually isolated in a microcapillary pipet, and the beta-cells were identified by amplificati

46 ed by a mixing cycle, is carried out with no pipeting, and a sample volume of 400 nL is sufficient fo

47 article dispersion, settling/centrifugation, pipetting, and visible-light spectroscopy, we developed

48 eater lateral distances than the size of the pipet aperture.

49 ly, when membranes contain Ni(2+) complexes, pipetting aqueous polyhistidine-tagged protein through t

50 p check valve, a diverter valve, and a 10-nL pipet are demonstrated.

51 other methods, issues related to incomplete pipetting are virtually eliminated.

52 ficient supernatant extraction is enabled by pipet aspiration at specific locations.

53 We report herein a novel pipet-based "ELISA in a tip" as a new versatile diagnost

54 We demonstrate a novel and versatile pipet-based approach to study the landing of individual

55 Overall, for the pipet-based deposition methods, results showed that the

56 CM), which is a technique that uses a mobile pipet-based electrochemical cell as an imaging probe.

57 For the two pipet-based sample deposition methods tested in this stu

58 , were compared in this study along with two pipet-based sample deposition methods, dried-droplet and

59 rea of carbon exposed to solution inside the pipet, both types of sensors yielded well-shaped voltamm

60 arable to the one obtained with conventional pipetting but with a significantly lower amount of start

61 unis can be made accessible to a patch-clamp pipet by removing a small portion (1-3 micrometers in di

62 ced whereby the two carbon electrodes in the pipet can be used to promote redox cycling of an individ

63 dispersed aggregated EVs, though subsequent pipetting caused reaggregation.

64 ts involving the same NP captured inside the pipet cavity.

65 ed using a "quad-probe" (four-channel probe) pipet cell, fabricated by depositing carbon pyrolyticall

66 xternal solution and collected at the second pipet ("collector"), (2) measurements of ohmic current-v

67 Using our pipet, complex paper-based microfluidic devices can be f

68 d the feasibility of automating the specimen-pipetting component of sample preparation in the LCx Chl

69 positioned above the nanopore as a change in pipet current.

70 DBS card were compared to volume-controlled pipetted DBS samples from the same finger prick.

71 s show a good agreement with measurements of pipetted DBS.

72 exhibit no substantial mean bias compared to pipetted DBS.

73 the concentration of chemoattractant in the pipet delivering chemoattractant.

74 A simple pipetting device was developed for reliable application

75 into sample reservoirs by using an eight-tip pipetting device, and the chip is docked with an array o

76 e of the small electrode area defined by the pipet dimensions, the background current is low, allowin

77 solution (<2 muL) and the MNPs (1-3 mug) by pipetting directly onto a matrix-assisted laser desorpti

78 rted to a dye-free solution using a transfer pipet, docked to a holding pipet, and held firmly during

79 After the pipet dosing was characterized in detail, two proof-of-c

80 ipets (subsequently called "dual superfusion pipet"), each providing the ability to dispense (i.e., t

81 positively charged heparin ionophores using pipet electrodes filled with the organic electrolyte sol

82 Novel dual-pipet electrodes prepared by pulling borosilicate theta-

83 Water-filled pipet electrodes were also used to study the stoichiomet

84 While linear multichannel pipets enable many established assays, they do not suppo

85 f electrolyte protruding from the tip of the pipet ensures that a gentle meniscus contact is made wit

86 A pipetting error of 10% during mixing of the premix and M

87 and bias, including the inner filter effect, pipetting errors, number of points in the Michaelis-Ment

88 r step causing inaccurate pipetting or total pipetting failure.

89 technique in which the probe is a fine glass pipet filled with a contact (reference) electrode and an

90 complexation reactions, with a multichannel pipet for adding the reagent and an infrared camera to m

91 combination with a programmable multichannel pipet form a highly efficient epitope mapping system.

92 range fluidic/electrical connections so that pipetting functions can be performed conveniently.

93 s waves are required for characterization of pipet geometry and precise determination of thermodynami

94 It is found that the pipet geometry plays an important role in controlling th

95 s slow diffusion of the DNA molecules in the pipet has also been observed.

96 Here, we develop a hand-held single-cell pipet (hSCP) that is rapid, operationally simple, highly

97 erent datasets from two different organisms, PIPETS identified significant 3'-end termination signal

98 5 muL of phosphate-buffered saline (PBS) via pipet immediately after Raman spectral measurements of t

99 ance to that of a calibrated, single-channel pipet in volume delivery experiments.

100 ter mix, and detection reagents with minimal pipetting, in a hand-held, disposable device intended fo

101 steps of cell lysis, labeling, washing, and pipetting inevitably triggers the cells, changes its bas

102 including plasma and urine, are deposited by pipet into a bed of CuO powder that fills a space within

103 m chloride, 1 mL of acetonitrile extract was pipetted into a 2-mL centrifuge tube containing anhydrou

104 aliquots of batch-cultured cells are rapidly pipetted into a hypotonic medium.

105 Samples are pipetted into an array of separable, multiplexed affinit

106 lacement or 150 mM mannitol or sucrose, were pipetted into cortical cups 35 min prior to ischemia and

107 (8 x 10(4)) and 3H-thymidine in medium were pipetted into each well to cover the treated and control

108 After the H(2)O(2)-TMB substrate was pipetted into the microfilter and catalyzed by the conju

109 e bacterial sample and immune Au@Pt NPs were pipetted into the mixing chamber and mixed sufficiently

110 s), in the end of a tapered micrometer-scale pipet is described.

111 study, because the meniscus of the scanning pipet is only in contact with the surface investigated f

112 The meniscus at the end of the pipet is sequentially placed at an array of locations ac

113 A patch clamp pipet is used to withdraw approximately 3 pL of cytoplas

114 Specifically, a dual-barrel pipet is used, where one barrel controls the pipet posit

115 PIPETS is a statistically informed, gene-annotation agno

116 faces surrounded by a thin glass wall of the pipets is enhanced by diffusion of the species from behi

117 The conical geometry of the pipet leads to a localized electric field, since all of

118 Samples are not pipetted manually but deposited by dragging one or sever

119 sily completed by either manual or automated pipetting methods.

120 The sensor is a pipet microtip preloaded with a segment of oil (plastici

121 d any blocking product is left behind as the pipet moves to a new location.

122 oss numerous 96 well plates and guidance for pipetting, non-trivial tasks for which informatics and v

123 ereafter "chicken matrix"), with just single pipetting of sample (i.e., no filtration, culturing and/

124 sample processing is required apart from the pipetting of the sample into a tin foil cup, which is pl

125 ts exerted by the charged inner surface of a pipet on IT processes.

126 Unlike pipetting, only sonication effectively dispersed aggrega

127 EMD ranging from 15 to 125 ng/5 microl were pipetted onto a 3-mm diameter x 2-mm thick bioabsorbable

128 vestigation, a raw urine sample was directly pipetted onto a triangular paper substrate, without any

129 Equal cell samples were pipetted onto calcium phosphate-coated disks in 24-well

130 For loading, the suspended microcrystals are pipetted onto the chip and excess mother liquor is subse

131 .8 containing 1% BSA and 0.05% Tween 20) and pipetted onto the sample-cum-conjugate pad.

132 estimates the sample height by up to several pipet opening radii.

133 omies of scale in reducing the complexity of pipetting operations.

134 dy-state counterpart obtained with a smaller pipet or at a slower scan rate.

135 A multichannel pipet or automated liquid-transfer system is used to tra

136 time-consuming motions with a single-channel pipet or device designs limited to the configurations of

137 nd are solely reliant on the speed of manual pipetting or a liquid handling robot.

138 ated sample-transfer step causing inaccurate pipetting or total pipetting failure.

139 as to promote or restrict migration from the pipet orifice.

140 es, one fluorescent reader, and no automated pipetting, over one thousand genotypes can be generated

141 ables DNA amplification with only the use of pipets, pipet tips, and a heater.

142 An unmodified, low-cost commercial robotic pipetting platform was utilized for one-pot sample prepa

143 We present PIPETS, (Poisson Identification of PEaks from Term-Seq d

144 pipet is used, where one barrel controls the pipet position and the second barrel houses voltage-gate

145 e technique that employs a dual-barrel theta pipet probe containing electrolyte solution and quasi-re

146 tacted by electrolyte is of the order of the pipet probe dimensions.

147 ork we use a "hopping mode", where the SECCM pipet probe is translated toward the surface at a series

148 rial on a commensurate scale to the employed pipet probe.

149 evice without loss of tumor cells during the pipetting process.

150 PIPETS provides a broadly applicable platform to explore

151 If one wishes to pipet reagents of less than 100 nL, new pipettors need t

152 for retrieval (fluid sampling) and addition (pipetting reagents or adding objects like tissue scaffol

153 First, an automated pipetting robot is commanded to prepare 286 nanocomposit

154 An automated pipetting robot is operated to prepare 264 mixtures of T

155 Media and drugs are supplied by a pipetting robot system.

156 To close this gap, we developed Lego-based pipetting robots that reliably handle liquid volumes fro

157 low-cost household consumables, programming pipetting routines, and modifying robot designs, we enab

158 hange (~ 4.4 mV) reached a peak 60 min after pipetting S. epidermidis plus 2% PEG-8 Laurate onto anod

159 herichia coli and Salmonella within a single-pipetted sample.

160 With manual pipetting, setting up a plate takes about 2 h, the initi

161 usly called 3'-end positions not captured by PIPETS showed that they were uniformly very low coverage

162 conducted at eight different combinations of pipet size and pressure.

163 osity) were unable to explain the effects of pipet size on the general aspiration rate.

164 r ATP; providing exogenous ATP (5 mM) in the pipet solution used during patch clamping did not recove

165 Finally, pipetting speed was significantly improved by using the

166 to be better than that obtained with manual pipet spotting.

167 mical gradients in culture, including puffer pipet spritzing and photolytic release of caged effector

168 ssay reagents, and automated using automated pipetting stations to increase the assay throughput.

169 highly sensitive, and requires only a single pipetting step.

170 erformed at the start of the assay (i.e., no pipetting steps are necessary during the assay).

171 lified, one-day LM-PCR protocol in which all pipetting steps can be performed by a robotic workstatio

172 ny recent techniques still involve laborious pipetting steps for spheroid manipulation such as collec

173 technique significantly reduces the numerous pipetting steps of spheroid manipulation to a single pip

174 eparation, capable of reducing the number of pipetting steps significantly, reducing reagent consumpt

175 Zebra BioDome reduced the number of pipetting steps to run the ScreenFire HPV assay.

176 voirs situated on the microchip in only five pipetting steps using an 8-channel pipettor.

177 distinct PCR reactions with only 2N + 1 = 41 pipetting steps, compared with the 3N2 = 1200 steps requ

178 or (rotational VFI) platform requiring fewer pipetting steps, designed for the electrochemical detect

179 ation microscopy analysis without additional pipetting steps.

180 less sample and an order of magnitude fewer pipetting steps.

181 trolled using a pair of concentric automated pipets (subsequently called "dual superfusion pipet"), e

182 e steady-state voltammograms of either IT at pipet-supported ITIES or electron transfer at solid elec

183 Steady-state voltammetry at the pipet-supported liquid/liquid interface has previously b

184 Our reconfigurable pipet supports the advancement of custom analytical tool

185 the pipet used, the applied voltage, and the pipet-surface separation.

186 methods require repetitive wash steps with a pipet that are laborious and time-consuming, making the

187 les prepared in laboratories using precision pipetting that resulted in consistent input volumes.

188 c ZnO NPs yield (4.63 g/L) was obtained when pipetting the mixed cell-free fractions with 100 mM of z

189 g steps of spheroid manipulation to a single pipetting; therefore, the errors from those steps are el

190 pepsin at known constant concentration were pipetted through an array of miniaturized chromatographi

191 d resilient; they self-heal when liquids are pipetted through them.

192 tion was achieved by scanning a 17 nm radius pipet tip at a distance as low as 1.3 nm from a highly p

193 mized through choice of appropriate affinity pipet tip derivatization chemistries.

194 a protease-modified membrane at the end of a pipet tip digests proteins in 30 s or less, and the shor

195 a nondestructive method for calibrating the pipet tip geometry by fitting the numerical model to the

196 droplet that is hanging from a (conductive) pipet tip in the organic filter phase.

197 Antibody digestion at the end of a pipet tip leads to 100% peptide coverage in MS analyses.

198 wo vesicles or to a vesicle on one end and a pipet tip on the other.

199 thods are applicable, including the use of a pipet tip or glass melting point tube, allowing integrat

200 x is excited by focusing a laser beam at the pipet tip to produce a submicrometer light source.

201 Nucleic acid is purified using a pipet tip with an embedded matrix that isolates nucleic

202 n, sol-gel materials were placed in a 10 muL pipet tip with glass wool on either side.

203 of ARPE-19 cells using a sequence-comb or a pipet tip, and it was allowed to heal in the presence or

204 into them using a syringe with a needle or a pipet tip, and then sealing the hole with nail hardener.

205 m weanling mice by oral wash using a plastic pipet tip, contains enough oral epithelial cells and lym

206 mples in a silica capillary, in a disposable pipet tip, in a polymer microchannel, or from samples de

207 magnetic plate with a stirrer, tubing, and a pipet tip, this easy-to-use device rivals the output of

208 )) are immobilized on the inner walls of the pipet tip, which serves as the assay compartment where s

209 c field guides these NA-PNA-beads toward the pipet tip, which they obstruct, leading to an indefinite

210 rehensive overview of recent developments in pipet tip-based analytical devices, highlighting their t

211 evolutionized modern chemical analysis, with pipet tip-based sensors emerging as versatile, low-cost,

212 nges during quasi-controlled breakage of the pipet tip.

213 IB) milling to precisely expose a UME at the pipet tip.

214 ding interface, and a nanoliter-to-picoliter pipet tip.

215 band at the very end of the interior of the pipet tip.

216 by diffusion of the species from behind the pipet tip.

217 subpicomole levels using this novel lab-in-a-pipet-tip approach.

218 We utilized pipet-tip ELISA to detect four cancer biomarker proteins

219 mpler platform utilizing low cost disposable pipet tips and conventional disposable microtiter well p

220 These materials are packed in pipet tips and in combination with a programmable multic

221 By repurposing standard plastic pipet tips as microreactors or sensor holders, these dev

222 fractions from 75-microm nanoLC columns into pipet tips at a user-defined, timed interval, typically

223 method involves sequentially moving rows of pipet tips containing sample solutions in close proximit

224 These clots plug the pipet tips during a manual or automated sample-transfer

225 he nanoLC fractions could be archived in the pipet tips for analysis at a later date.

226 ntial for combining functional membranes and pipet tips for rapid sample purification and pretreatmen

227 tools such as a technical pen and disposable pipet tips for the construction of the test and the cont

228 The hydrazide pipet tips have great potential in achieving automation

229 tions are then reconstituted directly in the pipet tips in approximately 250 nL of solvent prior to a

230 equipped with antibody-derivatized affinity pipet tips was used for the parallel extraction of speci

231 arge diameter Pt and sealed in polypropylene pipet tips were employed for end-column detection in CE.

232 A amplification with only the use of pipets, pipet tips, and a heater.

233 izing antibodies immobilized within affinity pipet tips, and eluted onto enzymatically active mass sp

234 mon laboratory materials, such as disposable pipet tips, filter paper, tooth picks, and nylon mesh.

235 rm parallel protein elution from 96 affinity pipet tips, proper buffering for on-target digestion, te

236 d of purification with reversed-phase-packed pipet tips.

237 thods that integrate membrane technology and pipet tips.

238 V) was injected into a micrometer-size glass pipet to construct an Ag+-selective SECM tip.

239 croscopy (P-SICM), that utilizes a nanoscale pipet to differentiate paracellular and transcellular tr

240 e-molecule unfolding by dosing acid from the pipet to locally melt individual molecules of duplex DNA

241 In this study, we use a nanoscale pipet to precisely locate tTJs within epithelia and meas

242 For this purpose, calcium was pipetted to graphite furnace together with samples.

243 es multiple centrifugation steps and careful pipetting to avoid loss of the bead-bound complexes.

244 The method involves using hand pipetting to create an array of cell-laden nanoliter-siz

245 0 microm and depended on the diameter of the pipet used, the applied voltage, and the pipet-surface s

246 his tool by modifying a commercial 8-channel pipet using machined and 3D-printed components.

247 ork, Na(+) or OH(-) ions were dosed from the pipet using pulsed voltage-driven delivery.

248 ameters of rapid IT processes extracted from pipet voltammograms.

249 ntribution decreases and other effects, like pipet volume uncertainty (typically >3%), dominate.

250 moderate-to-large copy number (N0 > 10(2)), pipet volume uncertainty and variability in the amplific

251 ed methodology for silanization of the outer pipet wall to investigate the kinetics of the rapid tran

252 that is initially present outside a tapered pipet was simulated by the finite element method, enabli

253 were displaced from the captured NA without pipetting wash buffers or use of external force and equi

254 around the cell, additional dual superfusion pipets were inserted into the microchannel for defined t

255 These pipets were shown to exhibit exceptional stability in ca

256 ys are performed manually using constriction pipets, which greatly limits the utility of this system.

257 mbryonic mice in vivo and study, using patch-pipet whole-cell current and voltage clamp techniques, s

258 ve developed a multichannel air displacement pipet with reconfigurable channels for nonstandard liqui

259 des have been prepared by masking PPC coated pipets with an insulating parylene C film.

260 be the fabrication of nanoengineered holding pipets with concave seating surfaces and fine pressure c

261 t-distance data and verify this method using pipets with opening radii between 30 and 300 nm.

262 id bilayers and a polycarbonate sample using pipets with opening radii down to 8 nm.

263 uidic ELISA system that supplants repetitive pipetting with rotationally controlled fluidics.

264 with simple sample recovery through vigorous pipetting with water.

265 cated rapidly from laser pulled theta quartz pipets, with the pH electrode prepared by in situ carbon

266 roplets are generated by manual or automatic pipetting without expensive and lab-bound microfluidic p

267 -well microtiter plate and automated using a pipeting workstation with performance suitable for high-

268 able, because variation in the amount of DNA pipetted would no longer contribute to variation in T/S,