ライフサイエンスコーパス: pipette

1 (volumetric flasks, graduated cylinders, and pipettes).

2 injection into the neuron through the patch pipette.

3 by infusion of GluA4 antibody through patch pipette.

4 r single cell RNA collected by a patch clamp pipette.

5 ear spiral, up to 180 mum from the recording pipette.

6 a(2+) is chelated by 10 mM EGTA in the patch pipette.

7 nts upon inclusion of Kir2.1-Ab in the patch pipette.

8 rnally and intracellularly through the patch pipette.

9 cell-attached patches through the recording pipette.

10 nts with trypsin added to the outside of the pipette.

11 5,10-imine maleate was included in the patch pipette.

12 earance of large vesicles around the puffing pipette.

13 f FXYD1 KO and WT myocytes through the patch pipette.

14 cinity of the dendritic whole-cell recording pipette.

15 GOmega) continuously pulls membrane into the pipette.

16 n 5-10min following cell break-in with patch pipettes.

17 e used to control injections from very small pipettes.

18 s adapters to fit most commercial 50-200 muL pipettes.

19 o automated sample handlers and multichannel pipettes.

20 ptimized microfluidic device integrated with pipettes.

21 with 200 muL of 0.9% NaCl buffer directly by pipetting.

22 samples and reagents are moved in and out by pipetting.

23 , followed by rinsing, we were able to reuse pipettes 10 times with no degradation in signal fidelity

24 g SLT-1B from complex cell lysates simply by pipetting 20 muL of the sample in and out of the tip in

25 elative standard deviation similar to manual pipetting (3.0% vs 1.4%).

26 s down to approximately 100 nL, although the pipetting accuracy and precision deteriorate considerabl

27 le trace amounts of Alconox remaining in the pipette after cleaning did not affect ion channel pharma

28 s practice of manually replacing patch-clamp pipettes after each recording.

29 blocker, 4-aminopyridine (4-AP, 5 mM) in the pipette also antagonised the effects of U6.

30 igh-throughput format using an eight-channel pipette and a homemade mini-heater, with a maximum throu

31 reagents are delivered using a multichannel pipette and a microwave reactor is used to complete pept

32 e a second pipette to cut off the tip of the pipette and add Matrigel to the center well." These erro

33 tions of ion currents through the tip of the pipette and appropriate positioning hardware provided a

34 usion of 100 microM diC8-PIP(2) in the patch pipette and bathing solutions, respectively, inhibited a

35 on of Kv2.1 antibodies through the recording pipette and extracellular application of rStromatoxin-1

36 First, unless the patch pipette and GUV pressures are precisely matched in the G

37 ucing the tip size, the ability to reuse the pipette and ion exchange with the cytoplasm.

38 rious [ATP], [InsP3] and [Ca2+] in the patch pipette and measured single InsP3R channel activity in s

39 Schematic shows brain slice, patch pipette and microscope objective.

40 blocked by phosphatase delivered within the pipette and not affected by treatment with the phosphata

41 oyment, requiring little more than a Pasteur pipette and not being affected by dilution factors.

42 ntents are aspirated through the patch-clamp pipette and prepared for RNA-sequencing.

43 es for field analysis since the multichannel pipette and the infrared camera are both operated with b

44 elized for 384 samples by using multichannel pipettes and 96-well plates.

45 iI were placed onto the tips of pulled glass pipettes and inserted into the inner nuclear layer of fi

46 en times smaller than typical microinjection pipettes and rather than pressure pulses as delivery met

47 table clamp, so on-chip operation only needs pipetting and adjusting of clamping force.

48 ple microwells thus avoiding repeated manual pipetting and costly robots.

49 Pipetting and dilution are universal processes used in c

50 ocular-surface epithelia can be isolated by pipetting and FACS sorting into a population of corneal

51 abricated using both manual liquid handling (pipette) and an automated liquid dispensing platform, th

52 article dispersion, settling/centrifugation, pipetting, and visible-light spectroscopy, we developed

53 ell in focus while iteratively adjusting the pipette approach trajectory to compensate for tissue mot

54 n to compensate for cell movement as a patch pipette approaches a targeted neuron.

55 ly, when membranes contain Ni(2+) complexes, pipetting aqueous polyhistidine-tagged protein through t

56 Existing pipettes are capable of delivering fluids with attolitre

57 By using a carbon pipette as the tip in the scanning electrochemical micro

58 gly curved cylindrical (tube) membrane and a pipette-aspirated giant unilamellar vesicle.

59 obtained that are consistent with both dual pipette aspiration and micropipette aspiration, a proble

60 allow as the approach angle of a patch-clamp pipette between a water immersion objective and the spec

61 elivered into rods and cones through a patch pipette, binds to and dissociates from synaptic ribbons.

62 mixing, the sample was transferred from the pipette bulb to a small diameter tube, and captured biom

63 included these two nucleotides in the patch pipette but found that TRPC5 currents were absent or wer

64 d for the two cell preparations at the lower pipette Ca2+ levels.

65 Whole cell K+ currents were determined at pipette [Ca2+] of 100 nM or 5 microM in the presence and

66 (length, 50-60 microm) at the tip of a glass pipette, can probe the intracellular environment with a

67 sed firing rate linked to an increase in the pipette-cell conductance.

68 We demonstrate the utility of pipette cleaning by developing the first robot to perfor

69 when applied onto plants in droplets with a pipette compared with a fine spray inoculation.

70 anger blocker KB-R7943, or with BAPTA in the pipette, consistent with a mechanism based on activation

71 mple was mixed within the bulb of a transfer pipette containing lyophilized nucleic acid-silica adsor

72 preconcentration, and clean-up method using pipettes containing immobilized metal ion affinity chrom

73 DBS card were compared to volume-controlled pipetted DBS samples from the same finger prick.

74 exhibit no substantial mean bias compared to pipetted DBS.

75 s show a good agreement with measurements of pipetted DBS.

76 of alphaSyn PFFs from whole-cell patch-clamp pipette decreased mEPSC frequency within 10 min followed

77 A simple pipetting device was developed for reliable application

78 ic depression and LTP inhibition after patch-pipette dialysis.

79 Intracellular delivery of GA via a patch pipette did not potentiate the acute effect of GA on Kir

80 ed or free fluorophores via whole-cell patch pipette did not support photopotentiation.

81 solution (<2 muL) and the MNPs (1-3 mug) by pipetting directly onto a matrix-assisted laser desorpti

82 ations by directly drawing micropillars from pipette-dispensed PDMS microdroplets using vacuum-chucke

83 vectors could be delivered through the patch-pipette during such recordings and that these vectors dr

84 Pipette effects on cell firing are traced to a combinati

85 obtained by rigidly fixing ('anchoring') the pipette electrode to the head; however, previous anchori

86 ole cell transduction currents through patch pipette electrodes at the basolateral membrane.

87 ast, and automated method for cleaning glass pipette electrodes that enables their reuse within one m

88 (3 microM) into the cell with the whole-cell pipette enabled AMPH-induced DA efflux at -60 mV in both

89 r infusion of autoactivated CaMKII via patch pipette enhanced chloride currents 3-fold, and this regu

90 A pipetting error of 10% during mixing of the premix and M

91 used into the pipette tip for the disposable pipette extraction (DPX) of carbendazim residues of oran

92 Schematic shows brain slice, patch pipette, field stimulation electrodes and microscope obj

93 Inclusion in the pipette-filling solution of a peptide corresponding to t

94 n of the Ca2+-specific chelator BAPTA in the pipette-filling solution or preincubation with the calci

95 Applying moderate suction to the pipette flattens the membrane, reducing tension, and mak

96 and transferred into lysate buffer by mouth pipette, followed by reverse transcription carried out d

97 range fluidic/electrical connections so that pipetting functions can be performed conveniently.

98 ative pressure (suction) applied through the pipette had no effect on the channel, but positive press

99 r of the second messenger cAMP via the patch pipette had no effect on THIK-1 currents.

100 Pipette-held test objects are translated perpendicularly

101 A and DAG lipase inhibitors in the recording pipette; however, unlike in PCs, NMDA receptors (NMDARs)

102 hole-cell recordings with Neurobiotin-filled-pipettes in horizontal slices from adult rat spinal cord

103 technique that rapidly (within 15 s) anchors pipettes in place with virtually no movement, thus subst

104 dicator fluo-4 in the whole cell patch clamp pipette, in addition to 20 mM EGTA and other constituent

105 ter mix, and detection reagents with minimal pipetting, in a hand-held, disposable device intended fo

106 With zero Na+ in the pipette INa inactivation produced a decline in the SR Ca

107 Pipette inclusion of protein kinase A (PKA) catalytic su

108 (L-GSH), through the intracellular recording pipette, indicating that the increased NMDAR response wa

109 ysis of DiC(8) PI(4,5)P(2) through the patch pipette inhibited Ca(2+)-dependent inactivation of TRPV6

110 tdIns(4,5)P2 or PtdIns(4)P through the patch pipette inhibited desensitization of TRPV1, indicating t

111 on of a small competing peptide in the patch pipette inhibited the SKF 81297-induced reduction in pea

112 he phosphoinositide 3-kinase pathway, in the pipette inhibited these effects.

113 dead-end nanoliter compartments using simple pipette injection, and quantify their individual secreti

114 e or saline or the insertion of the infusion pipette into the SI cortex of the right hemisphere.

115 m chloride, 1 mL of acetonitrile extract was pipetted into a 2-mL centrifuge tube containing anhydrou

116 aliquots of batch-cultured cells are rapidly pipetted into a hypotonic medium.

117 Samples are pipetted into an array of separable, multiplexed affinit

118 -attached mode, on pipette potential, and on pipette ionic composition.

119 A hollow patch pipette is attached to individual cells, enabling the re

120 A multi-pipette is the only additional equipment required for hi

121 The tip size of these pipettes is approximately ten times smaller than typical

122 nometres that resides at the apex of a sharp pipette: it provides scanning cryogenic thermal sensing

123 racellular Ni(2+), inclusion of BAPTA in the pipette, KB-R7943, and SKF96365.

124 Samples are not pipetted manually but deposited by dragging one or sever

125 the laboratory include traditional transfer pipettes, micropipettes based on air displacement, and m

126 hannel blocker applied through the recording pipette (MK-801).

127 ment by tracking cell position and adjusting pipette motion while approaching a target.

128 of a double-barrel (theta-glass) application pipette mounted on a piezo actuator.

129 dures were slow and often caused substantial pipette movement, resulting in loss of the recording or

130 to the above values, despite changes in the pipette Na(+) concentration, showing autoregulation of N

131 Removal of either extracellular or intra-pipette Na(+) had no effect on the selectivity, kinetics

132 oss numerous 96 well plates and guidance for pipetting, non-trivial tasks for which informatics and v

133 High concentrations of PIP(2) in the patch pipette not only resulted in a strong increase in sperm

134 3 and by intracellular dialysis from a patch-pipette of activated (thiophosphorylated) recombinant AM

135 influx was prevented by the presence in the pipette of the extracellular chaperone clusterin, which

136 oyl phosphatidylcholine (bis-DenPC) on glass pipettes of approximately 10 microm (I.D.).

137 ereafter "chicken matrix"), with just single pipetting of sample (i.e., no filtration, culturing and/

138 sample processing is required apart from the pipetting of the sample into a tin foil cup, which is pl

139 after saline infusion and by T = 3 h in the pipette only group.

140 manually dragging this liquid crystal from a pipette onto salty media, it is possible to extend this

141 EMD ranging from 15 to 125 ng/5 microl were pipetted onto a 3-mm diameter x 2-mm thick bioabsorbable

142 For loading, the suspended microcrystals are pipetted onto the chip and excess mother liquor is subse

143 we found that pungent chemicals added to the pipette or bath solution easily activated TRPA1 in cell-

144 r through direct Na(+) loading via the patch pipette or by focal application of glutamate (20 mM puff

145 sponse to cytosolic NADH loaded directly via pipette or increased by extracellular lactate perfusion,

146 many areas of science and technology, where pipettes or related devices are used for dispensing well

147 tion of different-sized vesicles through the pipette orifice followed by nanoelectrochemical analysis

148 ith the spatial resolution controlled by the pipette orifice radius and a few nanometers film thickne

149 y the nanoparticle translocation through the pipette orifice.

150 Patches creep up the pipette over time with voltage independent and voltage d

151 channels, local [Ca(2+)] imaging, and patch pipette perfusion of EGTA at the calyx of Held.

152 Cplx3 was introduced via a whole-cell patch pipette placed directly on the synaptic terminal, and ve

153 An unmodified, low-cost commercial robotic pipetting platform was utilized for one-pot sample prepa

154 Adaptive pipette positioning provides a platform for future advan

155 Making the pipette potential more positive leads to an increase in

156 te depends on time in cell-attached mode, on pipette potential, and on pipette ionic composition.

157 reversed the direction of creep at positive pipette potentials.

158 itutively active PKC delivered via the patch pipette potentiated NMDA (but not AMPA) whole-cell curre

159 eurons performed with high- and low-chloride pipettes (predicted GABA(A) receptor reversal potentials

160 in vitro multipatch setup with an automated pipette pressure and cleaning system facilitating record

161 The axial deformation of a pipette-pressurized fluid membrane bag produces minuscul

162 clusion of GDP-betaS (1 mM) in the recording pipette prevented all of the U6 effects, suggesting that

163 carboxylicacid hexyl ester] in the recording pipettes prevented modulation.

164 evice without loss of tumor cells during the pipetting process.

165 reshly prepared reagents within the transfer pipette produced similar results, suggesting that long-t

166 for retrieval (fluid sampling) and addition (pipetting reagents or adding objects like tissue scaffol

167 eflects rapid exit through membrane near the pipette recording site.

168 Here we used suction-pipette recordings from isolated ORNs of OMP(-/-) mice t

169 ell level as observed by single-cell suction pipette recordings.

170 apamin via the somatic whole-cell recording pipette reduced the medium afterhyperpolarization and in

171 eurons with natural Cl- concentration (patch pipette removed).

172 Media and drugs are supplied by a pipetting robot system.

173 To close this gap, we developed Lego-based pipetting robots that reliably handle liquid volumes fro

174 low-cost household consumables, programming pipetting routines, and modifying robot designs, we enab

175 Low pH pipette saline also increased E(a) and reduced the seal

176 High ionic strength pipette saline increased E(a) and, surprisingly, increas

177 hannel blocker MK-801 was added to the patch-pipette saline, suggesting that postsynaptically express

178 herichia coli and Salmonella within a single-pipetted sample.

179 Great advancements in harvest (in vivo pipette), sample preparation, and sequencing (Illumina H

180 of voltage between pairs of perforated-patch pipettes sealed onto abluminal cells located on microvas

181 With manual pipetting, setting up a plate takes about 2 h, the initi

182 Imaging with fluorescent dyes in the pipette showed that the hydrophilic dye Alexa 488 is imp

183 ch as projected cell area, adhesion area, or pipette size, as well as dynamical parameters such as th

184 the range approximately 0.4-4 mN/m, and the pipette size, one can precisely calculate the patch tens

185 ent was eliminated by removal of K+ from the pipette solution and partially blocked by the TASK (tand

186 against intracellular epitopes, in the patch pipette solution blocked TRPC1/C5 channel currents but p

187 with a steeper dependence on voltage if the pipette solution contains K(+) as the main cation than i

188 as high and elimination of it with K(+)-free pipette solution could not be reconciled with restricted

189 rotons by buffering HC cytosol with a pH 9.2 pipette solution eliminated feedback, whereas alkalinizi

190 Perfusion of ADP or control pipette solution had no effect, whereas perfusion of ATP

191 clamping cytosolic Ca(2+) concentration with pipette solution in which Ca(2+) was buffered to 1 micro

192 robustly inhibits ClC-1 when included in the pipette solution in whole cell patch clamp experiments a

193 Recordings made with an ATP- and GTP-free pipette solution produced large and robust TRPC5 current

194 T cells with 200 muM Navbeta4 peptide in the pipette solution to induce resurgent sodium currents.

195 Increasing pH buffering capacity in the pipette solution with 40 mm HEPES attenuated the effects

196 ump) (both at 10 and 100 mmol/L Na(+) in the pipette solution) and maximal NKA-mediated Na(+) extrusi

197 alpha5alphaP is normally applied through the pipette solution, addition of steroid to the bath soluti

198 um through inclusion of 750 nm Ca(2+) in the pipette solution, indicating that neither the calcium se

199 When EGTA was omitted from the pipette solution, the number of sparks triggered in KO a

200 dye from the cytoplasm with a dye-free patch pipette solution.

201 ould be reduced by inclusion of NH4Cl in the pipette solution.

202 ing the driving force by modifying the patch-pipette solution.

203 and eliminated in the absence of ATP in the pipette solution.

204 te, whereas others used an ATP- and GTP-free pipette solution.

205 hole-cell current densities are similar with pipette solutions containing cesium, potassium, or sodiu

206 of it when the Na(+) concentration in patch pipette solutions perfusing the intracellular compartmen

207 With intracellular pipette solutions that controlled free [Ca(2+) ]i , we f

208 ent when we included glutaredoxin 1 in patch pipette solutions to reverse glutathionylation.

209 ith 140 mM KCl and 1 mM Mg2+ in the bath and pipette solutions, two main open levels with conductance

210 gating were observed using MgATP or K2ATP in pipette solutions, which increases or decreases [Mg(2+)]

211 can produce currents similar to those in the pipette-spanning dome.

212 highly sensitive, and requires only a single pipetting step.

213 erformed at the start of the assay (i.e., no pipetting steps are necessary during the assay).

214 ny recent techniques still involve laborious pipetting steps for spheroid manipulation such as collec

215 technique significantly reduces the numerous pipetting steps of spheroid manipulation to a single pip

216 eparation, capable of reducing the number of pipetting steps significantly, reducing reagent consumpt

217 voirs situated on the microchip in only five pipetting steps using an 8-channel pipettor.

218 less sample and an order of magnitude fewer pipetting steps.

219 that TRPV4 can be activated by tens of mm Hg pipette suctions with open probability rising with sucti

220 e and GTPgammaS introduced through the patch pipette, suggesting that the two photoreceptor types emp

221 ied steroid compensates for the diffusion of pipette-supplied steroid out of the patch to the rest of

222 Pipette-supported single particle electrodes may also fi

223 ne allows the GPMV contents to passivate the pipette surface, thereby dynamically blocking membrane s

224 In the present work we used patch pipette techniques to study the properties of a novel Ca

225 scopy (FluidFM) is a recent force-controlled pipette technology that enables manipulation of single c

226 graphy scan was modified into a larger patch pipette that could be positioned with nanoscale precisio

227 s a nanometer-scale electrolyte filled glass pipette that serves as a scanning probe.

228 We describe quantum dot-coated glass pipettes that provide strong two-photon contrast at deep

229 With 5 mm Na+ in the pipette the reduction in release flux was greater (34 +/

230 roduce a Hewlett-Packard prototype picoliter pipette, the "thermal inkjet picofluidic system" (TIPS),

231 nce seal between a lipid bilayer and a glass pipette, the so-called "giga-seal", channel activity can

232 g steps of spheroid manipulation to a single pipetting; therefore, the errors from those steps are el

233 pepsin at known constant concentration were pipetted through an array of miniaturized chromatographi

234 d resilient; they self-heal when liquids are pipetted through them.

235 integrated the chemoenzymatic technique in a pipette tip (AutoTip) that was operated by an automated

236 in solid phase extraction (SPE) cartridge or pipette tip and coated on to the surface of magnetite fo

237 omeric avidin-coated microbeads trapped in a pipette tip and has been used for genotyping single nucl

238 t attainable separation distance between the pipette tip and substrate surface ( approximately 1 nm)

239 rom mdx mice are strongly curved towards the pipette tip by actin pulling normal to the membrane.

240 ification that allows controlled increase of pipette tip diameter.

241 ogically characterized and was used into the pipette tip for the disposable pipette extraction (DPX)

242 Based on these results, separations in micro-pipette tip format with these three types of stationary

243 The advantage of pipette tip SPE was reduced solvent volumes.

244 ime-consuming compared with conventional and pipette tip SPE.

245 ve read "Use a second pipette with a cut-off pipette tip to add Matrigel to the center well," instead

246 f reverse-phase resin packed at the end of a pipette tip to quickly separate unreacted AdoMet from ra

247 Both solid-contact and "pipette tip"-based sensors were successfully applied to

248 suspended by an atomic-scale meniscus at the pipette tip, and to image their phase transformations wi

249 xperimental setup, entirely built inside the pipette tip, is able to 1) block impurities/interference

250 example of an electrochemical biosystem in a pipette tip, namely bio-lab-on-a-tip.

251 s work, we evaluated the use of a disposable pipette tip, opportunely configured to demonstrate the f

252 e actuator can rapidly shift the theta-glass pipette tip, thus exposing the target receptors to alter

253 The combination of a pipette tip, wire electrodes, enzyme, and cotton wool fi

254 We develop a micro-pipette tip-based nucleic acid test (MTNT) for high-thro

255 size approximately given by the size of the pipette tip.

256 MTNT consists of micro-pipette tips and embedded solid phase nucleic acid extra

257 nzyme activity was moderate; and (iii) C(18) pipette tips and solvent extraction, a method that enric

258 ak hydrostatic pressures, with liquid-filled pipette tips as fluid columns at the inlets, to introduc

259 StageTips are ordinary pipette tips containing very small disks made of beads w

260 tential is hampered by poor visualization of pipette tips in deep brain tissue.

261 By immersing pipette tips into Alconox, a commercially-available dete

262 Pipette tips were designed and 3D printed as adapters to

263 hase polyamide resin (DPA-6S) in custom-made pipette tips.

264 with 1 mM fluo-3 and 3 mM EGTA via the patch pipette to buffer diadic cleft Ca2+, the number of spark

265 omised when BAPTA was added in the recording pipette to buffer intracellular Ca2+ and block the relea

266 light cures the thin adhesive layers linking pipette to collar to head.

267 o the center well," instead of "Use a second pipette to cut off the tip of the pipette and add Matrig

268 Here, we used a focal pipette to deliver minimal stimulus shocks near second-o

269 and IP(3) or Ca(2+) were delivered via patch pipette to one cell of a pair, or to a monolayer while f

270 of sample solution within the 12 tips of the pipette to perform a rapid analysis (1 min).

271 ed in the internal solution of the recording pipette to reduce possible effects of network inhibition

272 For this purpose, calcium was pipetted to graphite furnace together with samples.

273 Using patch pipettes to record whole-cell currents under voltage cla

274 We used patch pipettes to record whole-cell currents under voltage-cla

275 The method involves using hand pipetting to create an array of cell-laden nanoliter-siz

276 We demonstrated the pipettes' utility in targeted patch-clamp recording expe

277 of 14 pA in 74% of macropatches attached to pipettes (-V(p) = -60 mV) containing a low-Na(+), nomina

278 esults (positive/negative) for WB sampled by pipette versus swab were concordant for 78/79 (98.7%) WB

279 ate on Xpert to compare WB sampling methods (pipette versus swab); 43/71 BS specimens had been previo

280 d the lowest tension is encountered near the pipette wall.

281 High-resolution TEM of carbon pipettes was used to attain better understanding of the

282 were displaced from the captured NA without pipetting wash buffers or use of external force and equi

283 rrents is fast and uniform before whole-cell pipette washout, suggesting little voltage attenuation a

284 muM calcium into the calyx via a whole-cell pipette, we found that slow endocytosis was inhibited by

285 reactions inside the tips of a multichannel pipette were combined with temperature monitoring by an

286 tive or negative pressure from the recording pipette were considered to be transfected cells.

287 y stimulating the SNR close to the recording pipette, were inhibited to a smaller extent compared to

288 ts have included ATP and/or GTP in the patch pipette, whereas others used an ATP- and GTP-free pipett

289 h ions were loaded through the somatic patch pipette, which also recorded electrical responses.

290 Here, we demonstrate the operation of a pipette, which, observed by transmission electron micros

291 We used dual pipette whole cell patch clamp recording to measure the

292 A1 pyramidal neurons were loaded via a patch pipette with a Ca(2+)-sensitive indicator (fura-6F) and

293 ce in Step 33 should have read "Use a second pipette with a cut-off pipette tip to add Matrigel to th

294 forces arising from a flow through a conical pipette with a tip radius of 1-1.5 mum, placed approxima

295 show that supplementing the whole-cell patch pipette with PI(4,5)P(2) reduced inhibition of TRPM8 by

296 a servonull micropressure system, with glass pipettes with 2- to 3-microm tips used to cannulate epis

297 possibility of using nanometer-sized quartz pipettes with a layer of carbon deposited on the inner w

298 Here, we present a suite of glass pipettes with integrated microelectrodes for the simulta

299 different, and, therefore, carefully chosen pipettes with well-characterized geometry were necessary

300 able, because variation in the amount of DNA pipetted would no longer contribute to variation in T/S,