ライフサイエンスコーパス: planarian

1 gly similar to those guiding regeneration in planarians.

2 arkers and the strong negative phototaxis of planarians.

3 imited regeneration capacities of freshwater planarians.

4 uced genes during regeneration initiation in planarians.

5 the only proliferative cell type in asexual planarians.

6 ings suggest two distinct roles for TORC1 in planarians.

7 lie the remarkable regenerative abilities of planarians.

8 persisting regionalized expression in adult planarians.

9 mechanisms regulate germ cell development in planarians.

10 and axial polarity of regenerated tissue in planarians.

11 We characterized the Hh pathway in planarians.

12 uninjured regions of intact and regenerating planarians.

13 t allows us to follow stem cell migration in planarians.

14 ed as absent in free-living flatworms, e.g., planarians.

15 or male GSC specification and maintenance in planarians.

16 homeostasis and post-injury regeneration in planarians.

17 , and is up-regulated during regeneration in planarians.

18 In planarians, a pluripotent stem cell population and perpe

19 rved TALE class protein PBX/Extradenticle in planarians, a representative member of the Lophotrocozoa

20 ovel role for tec-1 as negative regulator of planarian adult neurogenesis.

21 ome-wide expression profiling of pluripotent planarian adult stem cells (pASCs), Onal et al present e

22 We determined that planarian and vertebrate excretory cells express homolog

23 he physiological origins of Kleiber's law in planarians and have general implications for understandi

24 es of stem cell and regeneration dynamics in planarians and Hydra.

25 unction of nanos in germ cell development in planarians and suggest that these animals will serve as

26 ng-lived free-living flatworms (for example, planarians), and neoblast-like cells have been described

27 yet sporadic and include some sponge, hydra, planarian, and salamander (i.e., newt and axolotl) speci

28 (hesl-3) and sim label progenitors in intact planarians, and following amputation we observed an enri

29 expression enriched in sexually reproducing planarians, and identified an orphan chemoreceptor famil

30 onic brains of polychaete annelids, polyclad planarians, and nemerteans.

31 Here, we show that planarians, and possibly other flatworms, lack centrosom

32 We propose that nr4A promotes pattern at planarian AP axis ends through restriction of patterning

33 Planarians are a group of flatworms.

34 Freshwater planarians are able to regenerate any missing part of th

35 Planarians are able to regenerate from essentially any t

36 ional regulatory mechanisms and suggest that planarians are an ideal model for this understudied aspe

37 Planarians are an important model organism for regenerat

38 Planarians are capable of regenerating any missing body

39 After amputation, planarians are capable of regenerating new anterior and

40 Planarians are capable of whole-body regeneration and mo

41 Planarians are flatworms and regenerate from tiny body f

42 Planarians are flatworms capable of regenerating all bod

43 Planarians are flatworms capable of regenerating all bod

44 Planarians are flatworms capable of regenerating any mis

45 Planarians are flatworms that constitutively maintain ad

46 Planarians are flatworms with robust regenerative capaci

47 Planarians are free-living flatworms capable of rapidly

48 ewly recognized virus-host system.IMPORTANCE Planarians are freshwater flatworms, related more distan

49 Planarians are regenerative flatworms that bidirectional

50 in of animal excretory systems and establish planarians as a novel and experimentally accessible inve

51 on or reproduction by fission, and establish planarians as a pertinent model for studying telomere st

52 gene controlling phagocytosis and establish planarians as a powerful system for analyzing host-patho

53 arity determinant notum Our work establishes planarians as a suitable model for further in-depth stud

54 k, suggesting that the bls family evolved in planarians as an additional mechanism for restricting ce

55 Our results establish planarians as an experimentally tractable animal model f

56 standard techniques, allowing for imaging of planarians at sub-cellular resolution in vivo using brig

57 Further, planarian Bcl-2 family proteins can induce and/or regula

58 with the last common ancestor of acoels and planarians being the ancestor of the Bilateria.

59 We identified 44 planarian bHLH homologs, determined their patterns of ex

60 y dynamic and reveals fundamental aspects of planarian biology that have been previously unappreciate

61 We identified a regional switch in the adult planarian body upon systemic disruption of homologous re

62 ession map exists for the maintenance of the planarian body.

63 alyses indicate that premeiotic functions of planarian boule2 and vertebrate Dazl evolved independent

64 se microRNAs in neuronal organization during planarian brain regeneration.

65 e role of the miR-124 family of microRNAs in planarian brain regeneration.

66 We performed RNA sequencing of planarian brain tissue following RNAi of hh and patched

67 findings indicate that, after decapitation, planarians build an organizing center from stem cells at

68 vidence of asymmetric stem cell divisions in planarians, but also demonstrate that EGF signaling like

69 maintaining and resetting axial polarity in planarians, but it is unclear how planarians reestablish

70 Planarians can be cut into irregularly shaped fragments

71 Planarians can regenerate any missing body part, requiri

72 Planarians can regenerate their entire CNS using pluripo

73 e collectively expressed in a broad range of planarian cell types, SL3 is highly enriched in a subset

74 use single-cell transcriptome sequencing on planarian cells to investigate the cell-type specificity

75 In planarians, centrioles are only assembled in terminally

76 tion, and identify additional components, of planarian chromatoid bodies.

77 s; however, these proteins are essential for planarian ciliogenesis.

78 ized, for the first time, glial cells in the planarian CNS that respond to injury by repressing sever

79 GABAergic, and octopaminergic neurons in the planarian CNS.

80 paration indicate that muscle functions as a planarian connective tissue, raising the possibility of

81 ell dynamics demonstrates the utility of the planarian digestive system as a model for elucidating th

82 Planarians display remarkable plasticity in maintenance

83 idered to have a centralized nervous system, planarians, display both abstinence-induced and antagoni

84 We used the planarian epidermal lineage to study how the location of

85 Our results further establish the planarian epidermis as a novel paradigm to uncover the m

86 The planarian epidermis is a simple tissue that undergoes ra

87 gical turnover, injury, and for some such as planarians, even amputation.

88 etiology is remarkably conserved between the planarian excretory system and the vertebrate nephron.

89 Here we focus on the planarian excretory system, which consists of internal p

90 Although planarians experienced a brief fall from grace, with the

91 Planarians famously can regenerate after decapitation.

92 Here, by generating an optimized planarian fission protocol in Schmidtea mediterranea, we

93 Here, we use the planarian flatworm as a simple chemical-genetic screenin

94 mprehensive model for regeneration, with the planarian flatworm being one of the most important model

95 The planarian flatworm is an emerging model that is useful f

96 enesis, it complements the historically used planarian flatworm models, such as Schmidtea mediterrane

97 sts contain pluripotent stem cells and drive planarian flatworm regeneration from diverse injuries.

98 to producing an ommochrome body pigment, the planarian flatworm Schmidtea mediterranea generates porp

99 zed the spatial expression of SL RNAs in the planarian flatworm Schmidtea mediterranea, with the goal

100 l transcriptome of Schmidtea mediterranea, a planarian flatworm that can regenerate all organs, inclu

101 rly unlimited regenerative capabilities make planarian flatworms an ideal system with which to invest

102 the slow diffusivity previously observed in planarian flatworms and housefly larvae.

103 The well-known regenerative abilities of planarian flatworms are attributed to a population of ad

104 Here, using planarian flatworms as a model system, we demonstrate th

105 Planarian flatworms contain a population of adult stem c

106 a previously unknown prey item (soft-bodied planarian flatworms in the genus Dugesia) made up the ma

107 Planarian flatworms regenerate every organ after amputat

108 Indeed, planarian flatworms were used as experimental models dec

109 Here we study the telomere biology of planarian flatworms with apparently limitless regenerati

110 In planarian flatworms, PIWI proteins are essential for reg

111 Freshwater planarians, flatworms from order Tricladida, are experim

112 ionship can be better understood by studying planarians, flatworms that continuously change their bod

113 ed the transcriptomes of major cell types of planarians--flatworms that regenerate from nearly any in

114 erentiate peptide dynamics in the freshwater planarian flatwormSchmidtea mediterraneaat different tim

115 Here we find that a planarian Follistatin homolog directs regeneration of an

116 ody plan and cell fate during embryogenesis, planarian Follistatin promotes reestablishment of anteri

117 ip (PIC) for rapid, stable immobilization of planarians for in vivo imaging without injury or biochem

118 hese difficulties, we present here Planform (Planarian formalization), a manually curated database an

119 iverse organisms, in particular neoblasts of planarians (free-living relatives of schistosomes).

120 ilized transcriptional profiling to identify planarian genes expressed in adult proliferating, regene

121 e protein families that are missing from the planarian genome.

122 Planarian glia and their regulation by Hedgehog signalin

123 Planarian glia were distributed broadly, but only expres

124 We propose that these cells are planarian glia.

125 and, therefore, defines a secondary role for planarian gonadal niche cells in promoting GSC different

126 receptor mining, we identified 566 putative planarian GPCRs and classified them into conserved and p

127 Our studies uncover the complement of planarian GPCRs and reveal previously unappreciated role

128 Planarians grow and regenerate organs by coordinating pr

129 ntrast to most well-studied model organisms, planarians have a remarkable ability to completely regen

130 Planarians have an abundant population of stem cells tha

131 of their exceptional regenerative abilities, planarians have become important models for understandin

132 posterior Wnt expression domains, controlled planarian head and trunk patterning.

133 A cell cluster at the anterior tip of planarian head blastemas (the anterior pole) is required

134 These very same neurons also produce the planarian hedgehog ligand (Smed-hh), which appears to co

135 evealing an important mitogenic role for the planarian hh signaling molecule in the adult CNS.

136 We have identified two planarian homeobox transcription factors, Smed-nkx2.1 an

137 embly of the morphogen gradients observed in planarian homeostasis and regeneration.

138 SmedOB1 in planarian, which is required for planarian homeostasis and regeneration.

139 main epithelial progenitor populations and a planarian homolog to the MEX3 RNA-binding protein (Smed-

140 l, we identify and functionally characterize planarian homologs of human DAZL/DAZ-interacting partner

141 We have developed the Planarian Immobilization Chip (PIC) for rapid, stable im

142 How the planarian immune system has evolved to cope with these p

143 nthesized in vitro We identified one of four planarian integrin-alpha subunits inhibition of which ph

144 s end, we analyzed the expression profile of planarian intestinal phagocytes, cells responsible for d

145 ed regionalization of gene expression in the planarian intestine along the medio-lateral axis, especi

146 indicate that growth and regeneration of the planarian intestine are achieved by co-ordinated differe

147 have analyzed growth and regeneration of the planarian intestine, the organ responsible for digestion

148 The asexual freshwater planarian is a constitutive adult, whose central nervous

149 ipulated and studied in vivo, the freshwater planarian is an ideal system with which to investigate t

150 Brain regeneration in planarians is mediated by precise spatiotemporal control

151 ne and amphetamine, but not cannabinoids, in planarians is mediated through a common nor-BNI-sensitiv

152 xperiments from the main publications in the planarian literature.

153 n functional regeneration experiments in the planarian literature; By analyzing all the datasets toge

154 ns of SmedOB1 provide one mechanism by which planarians maintain telomere and genome stability to ens

155 Here we produce an in silico planarian matrisome and use recent whole-animal single-c

156 a reciprocal manner with SLIT to pattern the planarian mediolateral axis, while WNT11-2 patterns the

157 ity of a high quality genome assembly of the planarian model species Schmidtea mediterranea, we provi

158 Using the planarian model system, we report that membrane voltage-

159 multiciliated epidermal cells in propelling planarian movement, as well as the osmoregulatory functi

160 er that SMEDWI-3 plays an additional role in planarian mRNA surveillance.

161 ntal pathways are predominantly expressed in planarian muscle cells.

162 tting and find unexpected roles for distinct planarian muscle fibers.

163 nuclear receptor expressed predominantly in planarian muscle, including strongly at AP-axis ends and

164 Most PCGs are expressed within planarian muscle; however, how muscle is specified and h

165 In planarians, muscle cells express signaling molecules to

166 Planarian neoblasts are pluripotent, adult somatic stem

167 These findings indicate that planarian neoblasts comprise two major and functionally

168 These cells resemble planarian neoblasts morphologically and share their abil

169 sine kinase tec-1 as a negative regulator of planarian neuronal regeneration.

170 We show that regenerating planarians' normal anterior-posterior pattern can be per

171 nd laterally in adult Schmidtea mediterranea planarians, opposing the dorsal-pole expression of Smed-

172 We find that either planarian or human TRPA1 can restore noxious-heat avoida

173 RNA-mediated genetic interference (RNAi) of planarian orthologues of human CKD genes and inhibition

174 tative analysis, we showed that after injury planarians perfectly restored brain:body proportion by i

175 regeneration assay in which ejection of the planarian pharynx is selectively induced by brief exposu

176 Here, we characterize planarian piRNAs and examine the roles of PIWI proteins

177 The planarian PIWI homologs SMEDWI-1 and SMEDWI-3 are requir

178 We find that the planarian PIWI proteins SMEDWI-2 and SMEDWI-3 cooperate

179 s directly controls stem cell-specific AS in planarians, placing the origin of this regulatory mechan

180 In planarians, pluripotent somatic stem cells called neobla

181 In planarians, positional control genes (PCGs) control rege

182 In planarians, positional information has been identified f

183 y be conserved and is an instructive part of planarian posterior regeneration.

184 Head-versus-tail regeneration in planarians presents a paradigm for study of this phenome

185 RNA interference-mediated depletion of planarian PRMT5 results in defects in homeostasis and re

186 on regulatory proteins that are required for planarian protonephridia regeneration.

187 Overall, our characterization of the planarian protonephridial system establishes a new parad

188 (frog, fish, mouse, and amphioxus) and from planarians (protostomes) suggest that Wnt signaling thro

189 Thus, planarians provide a powerful model to identify genes re

190 olarity in planarians, but it is unclear how planarians reestablish polarity signaling centers after

191 Planarians regenerate all body parts after injury, inclu

192 ally, we explored genes downregulated during planarian regeneration and characterized, for the first

193 milar to CHD4/Mi-2 proteins, as required for planarian regeneration and tissue homeostasis.

194 For example, planarian regeneration has been studied for over a centu

195 Planarian regeneration involves regionalized gene expres

196 possibility that an important early step in planarian regeneration is the specialization of neoblast

197 Axial patterning during planarian regeneration relies on a transcriptional circu

198 Planarian regeneration requires adult stem cells called

199 Planarian regeneration requires neoblasts, a population

200 erse-engineer the first mechanistic model of planarian regeneration that can recapitulate the main an

201 pable of searching for and finding models of planarian regeneration that match experimental data stor

202 Planarian regeneration uses a population of regenerative

203 In planarian regeneration, new cells arise from a prolifera

204 ts suggest that hnf4 is a regulatory gene in planarian regeneration, validate the computational predi

205 t by the reverse-engineered dynamic model of planarian regeneration.

206 the conserved JNK signalling cascade during planarian regeneration.

207 le in specifying posterior cell fates during planarian regeneration.

208 ive dynamical model explaining patterning in planarian regeneration.

209 de automated searches for unbiased models of planarian regeneration.

210 nd we demonstrate a broad requirement during planarian regeneration.

211 atin homolog (Smed-follistatin) required for planarian regeneration.

212 ns, we present a different kind of review of planarian regeneration.

213 In planarians, regeneration of entire animals from tissue f

214 ually curated database and software tool for planarian regenerative experiments, based on a mathemati

215 e developed a linear mechanical model with a planarian represented by a thin shell.

216 Asexual freshwater planarians reproduce by tearing themselves into two piec

217 tify a role for nuclear hormone receptors in planarian reproductive maturation and reinforce the sign

218 ns, SmedGD will prove useful not only to the planarian research community, but also to those engaged

219 This procedure will be useful for all planarian researchers, particularly those with relativel

220 our results demonstrate that where and how a planarian rips itself apart during asexual reproduction

221 ied six TTBK homologues in the genome of the planarian Schmidtea mediterranea (Smed-TTBK-a, -b, -c, -

222 micals elicit robust escape behaviors in the planarian Schmidtea mediterranea and that the conserved

223 Using the planarian Schmidtea mediterranea as a model, we identify

224 RNA interference of Smed-iguana in the planarian Schmidtea mediterranea caused cilia loss and f

225 dentify two boule paralogs in the freshwater planarian Schmidtea mediterranea Smed-boule1 is necessar

226 ashirt family of zinc-finger proteins in the planarian Schmidtea mediterranea to be a target of Wnt s

227 Here we used the ciliated epithelium of the planarian Schmidtea mediterranea to dissect the role of

228 We used the planarian Schmidtea mediterranea to study visual system

229 enome, and RNAi in the sexual biotype of the planarian Schmidtea mediterranea to test that hypothesis

230 rin-producing cells in the brown, freshwater planarian Schmidtea mediterranea Using an RNA-sequencing

231 In the planarian Schmidtea mediterranea, hedgehog (hh) is expre

232 ful in vivo model for stem cell biology, the planarian Schmidtea mediterranea.

233 m cells in a simultaneous hermaphrodite, the planarian Schmidtea mediterranea.

234 members of the Wnt signaling pathway in the planarian Schmidtea mediterranea.

235 e study of regeneration initiation using the planarian Schmidtea mediterranea.

236 tem cell division during regeneration in the planarian Schmidtea mediterranea.

237 upials), one amphibian and one flatworm, the planarian Schmidtea mediterranea.

238 Here, we report Kleiber's law scaling in the planarian Schmidtea mediterranea.

239 regulatory roles during regeneration in the planarian Schmidtea mediterranea.

240 own to regulate germ cell development in the planarian Schmidtea mediterranea; thus, we sought to inv

241 ons to work on neuropeptidergic signaling in planarian showed interesting parallels but also remarkab

242 y in a nutrient-rich environment; in starved planarians, silencing results in a decrease in cell size

243 cause of the unique phylogenetic position of planarians, SmedGD will prove useful not only to the pla

244 Some planarian species have remarkable regenerative abilities

245 However, planarian species with limited regenerative abilities ar

246 double-stranded RNA (dsRNA) viruses in five planarian species, including the well-characterized mode

247 vel taxon of dsRNA viruses in five different planarian species.

248 ollowed by full-length homologous pairing in planarian spermatocytes, is not observed in other specie

249 types and identify genetic regulators of the planarian stem cell system.

250 Asexual adult planarian stem cells appear to maintain telomere length

251 During regeneration, planarian stem cells are induced to form a cell populati

252 Here, we show that planarian stem cells directionally migrate to amputation

253 s, SL3 is highly enriched in a subset of the planarian stem cells engaged in regenerative responses.

254 (2016) show that a PIWI protein expressed in planarian stem cells is inherited by their differentiati

255 generation in which eye tissue production by planarian stem cells is not directly regulated by the ab

256 Planarian stem cells nearby the brain express core hh si

257 Our data reveal that the regulation of planarian stem cells relies on posttranscriptional regul

258 nown about the extrinsic signals that act on planarian stem cells to modulate rates of neurogenesis.

259 These findings suggest that planarian stem cells utilize molecular mechanisms found

260 s of chromatoid bodies and their function in planarian stem cells, and also support emerging studies

261 n uninjured organs, occurs in the absence of planarian stem cells, and can also be triggered by prolo

262 introns that is differentially regulated in planarian stem cells, and comprehensively identify its r

263 Planarian stem cells, or neoblasts, drive the almost unl

264 omatoid bodies to histone mRNA regulation in planarian stem cells.

265 Because planarians stop "doing it" at the slightest disturbance,

266 m grace, with the advent of molecular tools, planarians, such as Schmidtea mediterranea, have emerged

267 or Hofstenia muscle and this similarity with planarians suggests mesodermal muscle originated at the

268 -pbx (pbx for short), which encodes a second planarian TALE-family homeodomain transcription factor,

269 By providing a succinct overview of planarian taxonomy, anatomy, available tools and the mol

270 We present a cell-based model of planarian that can regenerate anatomical regions followi

271 Neoblasts are adult stem cells (ASCs) in planarians that sustain cell replacement during homeosta

272 In intact and regenerating planarians, the regulation of Wnt/beta-catenin signaling

273 Planarians thus present a fertile ground for the identif

274 he core components of the TOR pathway during planarian tissue homeostasis and regeneration and identi

275 MS ion images of planarian tissue sections allow changes in peptides and

276 nt of nerve axons distributed throughout the planarian tissue, and demonstrate that the head-tail axi

277 ls (ASCs) that facilitate the maintenance of planarian tissues and organs, providing a powerful syste

278 nsduction, demonstrate its conservation from planarians to humans, and imply that animal nociceptive

279 ere we used functional genomic approaches in planarians to identify genes required for proper germ ce

280 Thus, PIWI proteins enable planarians to repurpose piRNAs for potentially critical

281 Planarians undergo whole-body regeneration and tissue tu

282 Planarians use Wnt/beta-catenin signaling to polarize th

283 s not well understood, and only two types of planarian viruses have been described to date.

284 Characterization of the entire family of planarian voltage-operated Ca(2+) channel alpha subunits

285 Planarians, well known for their ability to undergo whol

286 oreover, DNA damage and apoptosis signals in planarian were significantly affected by SmedOB1 RNAi.

287 novel telomere-associated protein SmedOB1 in planarian, which is required for planarian homeostasis a

288 ersus-tail regeneration polarity decision in planarians, which requires Wnt signaling, provides a par

289 initial mechanistic studies of apoptosis in planarians, which revealed that a S. mediterranea homolo

290 In planarians, which scale reversibly over 40x through rege

291 These findings suggest that studies of planarians will inform our understanding of germ cell bi

292 Here we examine Procotyla fluviatilis, a planarian with restricted ability to replace missing tis

293 s a wealth of fascinating biology, including planarians with their astonishing regenerative abilities

294 g of the remarkable regeneration capacity of planarian worms and demonstrate the power of this automa

295 e same surgical and genetic experiments with planarian worms, obtaining the same phenotypic outcomes

296 ms with highly variable morphologies such as planarian worms, which due to their extraordinary regene

297 the PIC by performing time-lapse imaging of planarian wound closure and sequential imaging over days

298 Most planarian wound-induced genes are conserved across metaz

299 The gene activation program that occurs at planarian wounds to coordinate regenerative responses re

300 uality in live-imaged primary cell cultures, planarians, zebrafish and human cerebral organoids.