ライフサイエンスコーパス: plasmid vector

1 ivo gene transfection approach using a naked plasmid vector.

2 nonspecific effect of DNA insertion into the plasmid vector.

3 niquely placed in either strand of a shuttle plasmid vector.

4 1,500 bp were gel purified and cloned into a plasmid vector.

5 ed with the kanamycin resistance gene of the plasmid vector.

6 cDNA copy of BVDV NADL in a low-copy-number plasmid vector.

7 the output of RDA was shotgun cloned into a plasmid vector.

8 ol of a heterologous inducible promoter on a plasmid vector.

9 produced empty capsids when expressed from a plasmid vector.

10 ed in trans by the rfc gene expressed from a plasmid vector.

11 A-mediated type I IFN activation in a single plasmid vector.

12 esis gene BIO6 were introduced together on a plasmid vector.

13 G1a was expressed and purified using a novel plasmid vector.

14 ol, E. coli RecA was expressed from the same plasmid vector.

15 from a pool of PCR products and a linearized plasmid vector.

16 quences of the adeno-associated virus to the plasmid vector.

17 d be restored by reintroduction of cylE on a plasmid vector.

18 ovalent joining of DNA fragments to suitable plasmid vectors.

19 egration-free human iPSCs from blood MNCs by plasmid vectors.

20 ression system are contained in two separate plasmid vectors.

21 entially displayed bands were subcloned into plasmid vectors.

22 viral or bacterial recombinants, peptides or plasmid vectors.

23 caused by multisite integration of viral or plasmid vectors.

24 ach ORF were PCR amplified and inserted into plasmid vectors.

25 of a cotransfected gene relative to standard plasmid vectors.

26 d EST-specific mRNA and cloned directly into plasmid vectors.

27 e or RNase L alone and leaves containing the plasmid vector alone produced typical systemic infection

28 ) and high (H) levels of expression, and the plasmid vector alone was transfected into M12 as a contr

29 ected NIH3T3 cells or cells transfected with plasmid vector alone.

30 2 cDNA, compared to mice given injections of plasmid vector alone.

31 we synthesized Cryptosporidium DNA in clonal plasmid vectors, amplified them in different mock commun

32 d recombination in mammalian cells between a plasmid vector and a donor oligonucleotide was detected

33 tant improved expression from a conventional plasmid vector and from a Semliki Forest virus derived,

34 promoter region was cloned on an H. halobium plasmid vector and introduced into NRC-1 and S9, a bop o

35 excision of the transgene cassette from the plasmid vector and its permanent insertion into the geno

36 PCR products were cloned into a plasmid vector and recombinant clones identified by rest

37 restriction fragments of genomic DNA into a plasmid vector and screened for recombinant plasmids con

38 re incorporated into a single-strand shuttle plasmid vector and used to establish the mutational freq

39 Plasmid vectors and fluorescent protein reporter systems

40 genome with a composite Ori but lacking the plasmid vector, and a molecule consisting of the remaini

41 upon complementation with the sof gene on a plasmid vector, and heterologous expression of sof49 in

42 Random mutations in porB were generated in a plasmid vector, and mutant gene pools were transformed i

43 subsequently generated by PCR, cloned into a plasmid vector, and purified.

44 oduct is similar to that from the retroviral plasmid vector, and the representation of different inse

45 of H. ducreyi were cloned independently into plasmid vectors, and their encoded proteins expressed si

46 een bla and lacZ on the standard lacZ fusion plasmid vectors; and (6) the single-copy construct flank

47 These properties of EBV-based plasmid vectors appear to be due, at least in part, to t

48 cell types of the Arabidopsis root; seed and plasmid vectors are available through the Arabidopsis st

49 Plasmid vectors are considered to be a safer alternative

50 Recombinant plasmid vectors are versatile tools that have facilitate

51 g resistance, via target overexpression by a plasmid vector, as a selection tool.

52 The adoption of plasmid vector assembly standards and parts libraries ha

53 DMAb variants were encoded into a plasmid vector backbone and their expression and binding

54 Plasmid vector backbones expressing various RIG-I ligand

55 re user-friendly web interface and many more plasmid vectors, but also new links of the plasmids to a

56 from 100 to 200 CAGs in a yeast integrating plasmid vector by taking advantage of replication instab

57 The AAV genome cloned into a plasmid vector can also serve to initiate productive AAV

58 uorescent protein (GFP) from a microinjected plasmid vector can be suppressed in zebrafish embryos by

59 In addition, this novel plasmid vector can be used as a substrate for both rAAV

60 g a high-grade glioblastoma cell line with a plasmid vector capable of expressing an antisense transc

61 nipulation of C. diphtheriae, we constructed plasmid vectors capable of integrating into the chromoso

62 f green fluorescent protein (GFP) in which a plasmid vector carries a microsatellite repeat that plac

63 ransfected with empty plasmid vector or with plasmid vector carrying wild-type or mutant XRCC1 gene a

64 We constructed several plasmid vectors carrying different upstream and downstre

65 The expression of the plasmid vectors carrying the Rb C-box cDNAs was shown to

66 nt containing a single overhanging 3' A to a plasmid vector containing a 3' T.

67 ion of HEK-293 embryonic kidney cells with a plasmid vector containing a fluorescent protein tag.

68 region of the rpsL gene were inserted into a plasmid vector containing a promoterless xylE gene.

69 We generated a plasmid vector containing a six-His (6xHis)-tagged AAV V

70 e transcriptase PCR and were inserted into a plasmid vector containing a T7 promoter.

71 er ovary (CHO) cells were transfected with a plasmid vector containing the IL-1RI coding region.

72 nd in DTY167 transformed with a 2-micrometer plasmid vector containing YCF1.

73 istent with mutational spectra observed when plasmid vectors containing dG-AAF or dG-AF are introduce

74 Plasmid vectors containing Japanese encephalitis virus (

75 Three plasmid vectors containing the dG-C8-IQ adduct at the G1

76 caffold/matrix attachment region (S/MAR) DNA plasmid vectors containing the full-length human USH2A c

77 Plasmid vectors containing various fragments of this reg

78 flhA in trans, an flhA mutant containing the plasmid vector control, or an fliC mutant (nonmotile mut

79 eatment with the IFN-alpha1 transgene or the plasmid vector control, with 0% survival following HSV-1

80 infection than were cells transfected with a plasmid vector control.

81 lhA mutant with flhA in trans but not by the plasmid vector control.

82 or both rAAV vector production and synthetic plasmid vector delivery.

83 Expression of a recombinant F gene by use of plasmid vectors demonstrated that F contains its own tar

84 Integration-proficient plasmid vectors derived from phiRv1 efficiently transfor

85 e by repressor titration, using the new pORT plasmid vectors designed to facilitate recombinant DNA e

86 Taken together, the plasmid vector developed in this study permits the expre

87 y of the Cowden PEC genome was cloned into a plasmid vector directly downstream from the T7 RNA polym

88 ta/gamma delta TCR dKO mice treated with the plasmid vector DNA.

89 ocardial injection of angiogenic peptides or plasmid vectors during open heart surgery in patients.

90 can be corrected, and indicate the value of plasmid vector encoding appropriate chemokines to achiev

91 We evaluated whether a plasmid vector encoding CD154 (pCD40L) could influence t

92 ontrast to vectors encoding native beta-gal, plasmid vectors encoding beta-gal with a destabilizing r

93 der effect of the TRAIL gene, we constructed plasmid vectors encoding GFP-TRAIL or GFP-Bik chimeric p

94 Two plasmid vectors encoding the A and B subunits of cholera

95 y, the clpC gene cloned into a multiple-copy plasmid vector exhibited an activation phenotype, sugges

96 tion, cotransfection of HDV replicons with a plasmid vector expressing a hygromycin resistance marker

97 nescent dermal fibroblasts with a selectable plasmid vector expressing the SV40 T-antigen gene result

98 Here, using plasmid vectors expressing allelic human apoE2 or apoE3

99 s that had been transiently transformed with plasmid vectors expressing E proteins that were mutant i

100 Intratumoral injections of plasmid vectors expressing hpRNA for uPAR and cathepsin

101 However, plasmid vectors expressing large amounts of gene product

102 The originally described plasmid vectors expressing tTA/rtTA are driven by the cy

103 Transfection of a plasmid vector-expressing double-stranded RNA (dsRNA) fo

104 r by transfection of uninfected cells with a plasmid vector followed by infection with an F13L deleti

105 ine sequence was expressed from a eukaryotic plasmid vector following transfection into COS-1 cells.

106 The most commonly used plasmid vector for this purpose is L4440.

107 While constructing a plasmid vector for transfection of trypanosome cells, we

108 We also describe a set of "pEarleyGate" plasmid vectors for Agrobacterium-mediated plant transfo

109 ents (HRSE) was incorporated separately into plasmid vectors for generation of self-complementary ade

110 We report the development of a series of plasmid vectors for the construction of fusions to mutan

111 We have constructed a series of plasmid vectors for the expression of foreign genes in i

112 iously described to provide a large suite of plasmid vectors for use in this and other related Gram p

113 atest transformation efficiencies, while the plasmid vector had no significant effect, nor did the de

114 A plasmid vector harbouring this minimal 71 bp oriT was mo

115 To this end, a plasmid vector has been prepared featuring the polylinke

116 Herpes simplex virus type 1 (HSV-1) plasmid vectors have promise for genetic intervention in

117 All four eib genes, when cloned into plasmid vectors, impart IgG binding to E. coli K-12 stra

118 ts in a defined order and insert them into a plasmid vector in a single recombination reaction.

119 ia psittaci genomic library in a pBluescript plasmid vector in vitro, trapping EUO-bound plasmid clon

120 on in vitro and transferred into E. coli via plasmid vectors in the absence of the cognate methylase.

121 ave used EM visualization of active genes on plasmid vectors in Xenopus oocyte nuclei to investigate

122 These limitations include unwieldy plasmid vectors, incomplete or poorly designed two-hybri

123 in software for codon selection (CodonZ) and plasmid vectors incorporating the most active non-coding

124 xic response following the delivery of these plasmid vectors into mammalian hosts.

125 nsduction of mecA from K1M200 (cloned into a plasmid vector) into a methicillin-susceptible S. aureus

126 uggest that systemic administration of naked plasmid vector is a convenient, safe, and highly efficie

127 red because when cloned on a low-copy-number plasmid vector it was able to suppress the temperature-s

128 When this fragment is subcloned into a plasmid vector, it facilitates the site-specific integra

129 COS-1 cells transformed with this plasmid vector (JE-4B clone) secreted JEV-specific extra

130 Our finding that traditional rAAV plasmid vectors lack integration potency compared to wtA

131 ession of the protein in feline cells from a plasmid vector made them largely resistant to FPV infect

132 ts was hindered by CTG repeat instability in plasmid vectors maintained in Escherichia coli and poor

133 and precise transfer of DNA segments between plasmid vectors, makes this technology ideal for genomic

134 Increasing levels of plasmid vector-mediated activation of innate immune sign

135 Binary Ti vectors are the plasmid vectors of choice in Agrobacterium-mediated plan

136 producing each E. coli protein from the 4287 plasmid vectors of the ASKA library and selecting for in

137 ells and EM-C11 cells transfected with empty plasmid vector or with plasmid vector carrying wild-type

138 was able to support replication in cis of a plasmid vector otherwise unable to replicate, if an inta

139 Co-expression of this plasmid with a second plasmid vector over-expressing the E. coli chaperonin pr

140 thelial cells were transfected in vitro with plasmid vector pcDNA:IGF-1, which encodes an epitope-tag

141 P-selectin, were cloned into a pUC19-derived plasmid vector, pCW93, and a pACYC184-derived phagemid v

142 re stably transfected with a hFIX expression plasmid vector, pdLMe4 betaA-hIXm1, which contains a hFI

143 xanthus phage Mx8 genome, when cloned into a plasmid vector, permits site-specific integration of the

144 efore being cloned into the Escherichia coli plasmid vector pET-28a (+) at the EcoRI and EcoRV restri

145 The plasmid vector pGEM11 yielded clones ranging in insert s

146 One conventional plasmid vector (pGEM11), one conventional binary plasmid

147 A plasmid vector (pHEBo) containing cDNA encoding the HLA-

148 A packaging plasmid/vector plasmid system containing no significant

149 shRNA lentiviral plasmid vectors pLSLP-HK1, pLSLP-HK2, and pLSLP-HK3 were

150 omal Deltaasd mutation was complemented by a plasmid vector possessing the asd+ gene.

151 yeast with the PCR fragment and a linearized plasmid vector prepared such that the ends of the vector

152 by expression of the URE gene in a mammalian plasmid vector (pSecTag2A.URE) which was used to immuniz

153 mid vector (pGEM11), one conventional binary plasmid vector (pSLJ1711) and one conventional binary co

154 njectable muscle-specific synthetic promoter plasmid vector (pSP-HV-GHRH), elicits growth in pigs.

155 The oprF gene was cloned into plasmid vector pVR1020, and the plasmid vaccines were de

156 n an antisense orientation in S. aureus on a plasmid vector reduces alpha-toxin production and the le

157 an intact copy of the S. iniae pgm gene on a plasmid vector restored antimicrobial peptide resistance

158 introduction of the nine-gene sag locus on a plasmid vector restored SLS activity to the nonhemolytic

159 nhibit TGF-beta expression with an antisense plasmid vector resulted in a significantly higher number

160 venous injection of human HGF gene via naked plasmid vector resulted in abundant HGF protein specific

161 eplication was an AAV genome inserted into a plasmid vector, RPA was not an effective substitute for

162 taining varying amounts of flanking gene and plasmid vector sequences were then introduced as linear

163 the bacterial neomycin-resistance gene in a plasmid vector, such that the reading frame of the neo g

164 Thus, this EBV-based plasmid vector system both markedly prolongs gene expres

165 A plasmid vector, termed pSG5rab.gp, expressing the glycop

166 ey as a convergent fusion into one bacterial plasmid vector that can then be amplified and paired-end

167 In this study, we describe the use of a plasmid vector that confers high G6PC2 protein expressio

168 2 glycoprotein D (gD), using combinations of plasmid vector that expresses gD (pgD2) and a recombinan

169 -KBP following transient transfection with a plasmid vector that expresses murine CD40, but not when

170 on junction of circHIPK3 or elevated using a plasmid vector that overexpressed circHIPK3.

171 between 3 and 5 kbp in length on a multicopy plasmid vector that was transformed into E. coli to scre

172 We evaluated delivery of 0.1-3 mg of plasmid vectors that encode reporter secreted-embryonic

173 Here, we describe plasmid vectors that facilitate the construction of high

174 ed reagents, we have constructed a series of plasmid vectors that permit expression of amino-terminal

175 A cloning method and plasmid vectors that permit fluorescence-anisotropy-base

176 oli cells transformed with any of the common plasmid vectors that provide ampicillin resistance throu

177 macrophages are difficult to transfect with plasmid vectors, these studies illustrate that primary m

178 rate cell type-specific responses to the DNA plasmid vector, they show no evidence of an immunogenic

179 flaviviruses, we have developed a eukaryotic plasmid vector to express the premembrane/membrane and e

180 method can be applied to selectively deliver plasmid vectors to the heart.

181 roup (0/8) compared with levels expressed in plasmid vector-treated controls (4/6 mice surveyed were

182 d the infection to a greater extent than the plasmid vector-treated counterpart and at a level simila

183 ia virus type 1 (HTLV-1) was inserted into a plasmid vector under the control of the SP6 promoter.

184 Moreover, the clones include plasmid vectors useful for routine and cutting-edge tech

185 disrupted is amplified by PCR, cloned into a plasmid vector using topoisomerase and then employed as

186 and pre-S genes encoded on an Asd+ pBR-based plasmid vector was constructed.

187 A versatile plasmid vector was designed to direct the synthesis of r

188 A plasmid vector was designed, constructed, and used for t

189 A plasmid vector was developed that permitted high-level e

190 A second plasmid vector was generated that contained the full-len

191 uberculosis DNA cloned upstream of inhA in a plasmid vector, was electroporated into M. tuberculosis,

192 Each of two ORFs, cloned into a plasmid vector, was inactivated with this cassette.

193 Through weekly repeated injections of plasmid vector, we achieved sustained, long-term, high l

194 Using an antisense (AS)-CAR plasmid vector, we silenced surface CAR expression in lu

195 Using asd-based plasmid vectors, we designed SC608 to express the entero

196 of S. salivarius 57.I in an Escherichia coli plasmid vector were identified.

197 Plasmid vectors were tested in live mammalian cells to s

198 in was sufficient for the replication of the plasmid vector when Rep and adenovirus (Ad) helper funct

199 Using a commercially available plasmid vector with a luciferase reporter gene already i

200 were co-expressed in Escherichia coli on one plasmid vector with each cDNA having its own IPTG-induci

201 IS903phikan was placed on an IncQ plasmid vector with the transposase gene located outside

202 hnique to clone single oligonucleotides into plasmid vectors with high efficiency that predictably re

203 the loss frequencies of some low copy number plasmid vectors with parS inserts, as well as that of P1

204 sporting such hormones when overexpressed on plasmid vectors (with some demonstrable specificity obse

205 ontrols, mice were immunized with either the plasmid vector without an osp-coding sequence or recombi

206 ess high levels of immunogenic antigens from plasmid vectors without the need for antibiotic selectio