ライフサイエンスコーパス: poly (A) tail

1 single polyadenylation site 229 nt from the poly-A tail.

2 canonical 5' 7-methylguanosine cap and a 3' poly-A tail.

3 capture of information distant from the mRNA poly-A tail.

4 MKIIalpha protein and mRNA by shortening its poly(A) tail.

5 nd cytoplasmic lengthening of the neg-1 mRNA poly(A) tail.

6 n the renal outer medulla appeared to lack a poly(A) tail.

7 precursor, cleaves at that site, and adds a poly(A) tail.

8 leolytic cleavage followed by synthesis of a poly(A) tail.

9 codons or translational readthrough into the poly(A) tail.

10 tly translated in vivo despite the lack of a poly(A) tail.

11 nding that PUMs repress an mRNA that lacks a poly(A) tail.

12 e U-rich internal loop of the ENE and the 3'-poly(A) tail.

13 cription or translation or the status of the poly(A) tail.

14 capping by the addition of a CUCU tag to the poly(A) tail.

15 that hybridizes to and protects the PAN RNA poly(A) tail.

16 ling in ribosomes on translation through the poly(A) tail.

17 pended upon the mRNA's 5' cap but not its 3' poly(A) tail.

18 s end in a conserved stem-loop rather than a poly(A) tail.

19 t recognize the cis-elements and produce the poly(A) tail.

20 ndent on the length of both the mRNA and the poly(A) tail.

21 nterplay between the 5' m(7)G cap and the 3' poly(A) tail.

22 X(L) mRNA was dependent upon the presence of poly(A) tail.

23 PEB, but after polyadenylation, it binds the poly(A) tail.

24 g in the stem-loop but not a reporter with a poly(A) tail.

25 ot distinguish RNAs with or without a cap or poly(A) tail.

26 mRNAs in that they possess a 5' cap and a 3' poly(A) tail.

27 POP2 levels induce a lengthening of tim mRNA poly(A) tail.

28 aves pre-mRNAs at a specific site and adds a poly(A) tail.

29 in histone transcripts which typically lack poly-A tails.

30 ed with maintenance and/or extension of long poly(A) tails.

31 le for mixed tailing and protection of viral poly(A) tails.

32 4 with mRNAs containing critically shortened poly(A) tails.

33 of single-stranded, positive-sense RNAs with poly(A) tails.

34 ust circadian rhythms in the length of their poly(A) tails.

35 these RNAs while stabilizing mRNAs with long poly(A) tails.

36 iduals were shown to have severely truncated poly(A) tails.

37 uncated AU-rich mRNAs lacking the 3' UTR and poly(A) tails.

38 nto structures previously thought to be long poly(A) tails.

39 is known to trim hTR precursors by removing poly(A) tails.

40 A and mRNA; and the Distal Site, which binds poly(A) tails.

41 -seq that directly sequences the full-length poly(A) tails.

42 ter transcripts lack splicing signatures and poly(A) tails.

43 pore complexes and the length of transcript poly(A) tails.

44 ly(A) tails while other mRNAs to have longer poly(A) tails.

45 bundance of the mRNA subpopulation with long poly(A) tails (a rough proxy for mRNA translatability).

46 processes, it is important to identify these poly(A) tails accurately in transcriptome sequencing dat

47 In eukaryotes, the poly(A) tail added at the 3' end of an mRNA precursor is

48 of a targeted gene, subsequently inhibiting poly(A) tail addition and leading to degradation of that

49 on in most eukaryotes, involves cleavage and poly(A) tail addition at the 3' end of mRNAs at the poly

50 of U1 snRNP to the target pre-mRNA inhibits poly(A)-tail addition, causing degradation of that RNA s

51 ansport and translation, suggesting that the poly(A)-tail also provides a basis for eukaryotes to eff

52 cells accomplish similar decoupling through poly(A) tail alterations to ensure that dormant transcri

53 HTTAS is 5' capped, poly (A) tailed and contains three exons, alternatively

54 ells, the oocyte transcriptome has a shorter poly(A) tail and a higher relative proportion of termina

55 down cells, p53 mRNA has an abnormally short poly(A) tail and a reduced translational efficiency, res

56 on of an mRNA is strongly impacted by its 3' poly(A) tail and associated poly(A)-binding proteins (PA

57 nteraction of topoisomerase IIalpha with the poly(A) tail and G/U-rich 3'-untranslated region (3'-UTR

58 nome of TV contains 6,714 nucleotides plus a poly(A) tail and is organized into three open reading fr

59 FLuc production rate that was dependent on a poly(A) tail and poly(A)-binding protein, but was indepe

60 tramolecular RNA clamp, sequestering the PAN poly(A) tail and preventing the initiation of RNA decay.

61 s between polysomes and P-bodies and how the poly(A) tail and the associated poly(A) binding protein

62 proximity of the termination codon to the 3' poly(A) tail and the poly(A) RNA-binding protein, PAB1,

63 nctionally replaced interactions between the poly(A) tail and the poly(A)-binding protein (PABP) to a

64 As: efficiently translated mRNAs have longer poly(A) tails and are shorter, more stable, and more eff

65 can therefore interact with mRNAs via their poly(A) tails and caps, as well as through sequence-spec

66 cells into two fractions with short and long poly(A) tails and compared them by microarray analysis.

67 ocol that queries the junctions of 3'UTR and poly(A) tails and confidently maps the poly(A) tags to t

68 form enhances translation and elongates the poly(A) tails and imparts its translational state to the

69 eotides to capture the RNAs at their natural poly(A) tails and initiate sequencing by synthesis.

70 either GLD-2 or RNP-8 resulted in shortened poly(A) tails and lowered abundance of four target mRNAs

71 length, while GC-rich Alus only in their 3' poly(A) tails and middle A-stretches, with differences d

72 ource tool for finding the precise border of poly(A) tails and other homopolymers in raw mRNA sequenc

73 n that highly expressed genes can have short poly(A) tails and the elucidation of the seemingly contr

74 levels of CDK11 increased the length of HIV poly(A) tails and the stability of mature viral transcri

75 majority of these products contained intact poly(A) tails and were bound by the poly(A) binding prot

76 n RNA during cDNA synthesis by aiming at the poly(A)+-tail and (2) introduced a pre-amplification of

77 rphic Alus, TypeTE identifies the hallmarks (poly-A tail and target site duplication) and orientation

78 ion, independent of 7-methylguanylate cap or poly(A) tail, and prompts mRNA redistribution to silenci

79 inding protein N1 (PABPN1) and PABPC1 at the poly(A) tail, and, provided biogenesis involves pre-mRNA

80 nscripts with a high frequency of very short poly(A) tails, and a loss of 3' oligo-uridylation.

81 L1/MALAT RNAs accumulate in cells, lack poly(A) tails, and are translated; however, they cannot

82 a mice have mild histologic defects, shorter poly(A) tails, and evidence of mitochondrial damage.

83 peroxidase (HRP)-biotin/streptavidin to the poly(A) tails, and the oxidation reaction of tetramethyl

84 ith much less starting material, the lack of poly(A)-tails, and the fact that the messages can be pol

85 he first study to reveal that TATA boxes and poly (A) tails are direct targets for BBR in its regulat

86 esent study demonstrates that TATA boxes and poly (A) tails are the first and second primary targets

87 Poly(A) tails are 3' modifications of eukaryotic mRNAs t

88 mRNA poly(A) tails are important for mRNA stability and trans

89 Therefore, we propose that mRNA poly(A) tails are important in regulating protein synthe

90 Following the resumption of meiosis, the poly(A) tails are lengthened and translation ensues.

91 Poly(A) tails are non-templated additions of adenosines

92 new insights into how transcripts that lack poly(A) tails are stabilized and regulated and suggest t

93 Here we identify a terminal poly(A) tail as being important for a subset of intron e

94 rity of both forms requires the mRNA cap and poly(A) tail, as well as eIF4E, eIF4G, Pab1 and eIF3, an

95 paving the way for a better understanding of poly(A) tail-associated regulation of gene expression.

96 ulatory regions as well as the poly adenine (poly (A)) tail at the mRNA terminus.

97 transcripts have a 5' monophosphate, lack a poly(A) tail at the 3' end, and contain no introns; thes

98 ssenger RNA (mRNA) does not terminate with a poly(A) tail at the 3'-end.

99 timulated hypothalamus shortens the AVP mRNA poly(A) tail at the same time as reducing transcript abu

100 ions of 3'-untranslated region (UTR) and the poly(A) tails at the transcriptome level, a comprehensiv

101 We uncover a poly(A) tail-based regulatory mechanism that dynamically

102 tic initiation factor 4E (eIF4E), eIF4G, and poly(A) tail-binding protein (PABP) that circularizes mR

103 cal structure that could not only shield the poly(A) tail but also serve as a scaffold for the assemb

104 ctor 4E (eIF4E) at the cap and PABPC1 at the poly(A) tail, but that lacks detectable EJCs and PABPN1.

105 ranscribed by Pol II and acquire 5' caps and poly(A) tails, but only mRNAs are translated into protei

106 Coating of the poly(A) tail by mtPABP1, however, did not lead to transc

107 obe DNAs, extension reaction of polyadenine (poly(A)) tails by poly(A) polymerase, binding of a terna

108 We demonstrate that mRNA in yeast lacking a poly(A) tail can be destabilized by introduction of a pr

109 ion of mRNAs, consistent with alterations in poly(A) tail chain termination.

110 end in a stem-loop structure instead of the poly(A) tail characteristic of all other mature mRNAs.

111 Many plant viruses without 5' caps or 3' poly(A) tails contain 3' proximal, cap-independent trans

112 More than 10% of poly(A) tails contain at least one guanosine (G); among

113 w dwarf virus RNA, lacking a 5' cap and a 3' poly(A) tail, contains a cap-independent translation ele

114 ion in myoblasts led to a shortening of mRNA poly(A) tails, demonstrating the cellular function of PA

115 ing proteins directly recruit PABP, in a non-poly(A) tail-dependent manner, to stimulate the small su

116 supports export and translation as well as a poly(A) tail does.

117 eadenylases are best known for degrading the poly(A) tail during mRNA decay.

118 ranscription can initiate upstream of the 3' poly-A tail during retrotransposon integration.

119 tion motif of HuD, the domain that binds the poly(A) tail, eliminated the branch-specific expression

120 The WISP protein is required for poly(A) tail elongation of bicoid, Toll, and torso mRNAs

121 specific genes through deadenylation of mRNA poly(A) tails, enabling positive selection.

122 We thus expect that a translated poly-A tail, encoding for positively charged lysines reg

123 rithms struggle to accurately identify these poly(A) tail end-points.

124 Poly(A) tails enhance the stability and translation of m

125 ally, we show that neither a 5' cap nor a 3' poly(A) tail enhances Sec incorporation.

126 While greater distances with the poly(A) tail exacerbate dependency on PABP for deadenyla

127 to 3' stem-loop structures not involving the poly(A) tail exhibit even longer half-lives.

128 rase WISPY is responsible for stage-specific poly(A) tail extension in the female germline.

129 ware of focus exclusively on the trimming of poly(A) tails, failing to provide the detailed informati

130 in part through the removal of PAB1 from the poly(A) tail following its self-association into multime

131 nterestingly, REF/Aly appears to protect the poly(A) tail from deadenylation, and REF/Aly-stabilized

132 engths have impeded greater understanding of poly(A)-tail function.

133 In eukaryotes, poly(A) tails generally stabilize mature mRNAs, whereas

134 se R, but can be efficiently degraded once a poly(A) tail has been added to their ends.

135 w dwarf virus mRNA, which lacks both cap and poly(A) tail, has a translation element (3'-BTE) in its

136 pair DNA hairpin attached to a 50-nucleotide poly-A tail (HP-A(50)) is threaded into an alphaHL chann

137 polyadenylation or retain a reasonably long poly(A) tail if they are to return to the translating po

138 n vivo, arguing against a major role for the poly(A) tail in microRNA-mediated silencing.

139 n may be relevant, as the positioning of the poly(A) tail in mRNAs influences the length of the 3'-un

140 earch for the enzymes responsible for adding poly(A) tails in Chlamydomonas and Arabidopsis organelle

141 dentified 213 transcripts that have extended poly(A) tails in Noc KO liver.

142 D(pol) that resulted in shorter or longer 3' poly(A) tails in virion RNA.

143 ll as the vast majority of species that lack poly-A tails in their mRNAs (including all archea and ba

144 the first example of a cellular IRES that is poly(A) tail-independent.

145 t is a polyU sequence that can interact with poly(A) tails, inhibit the association of poly(A)-bindin

146 e eukaryotic initiation factor (eIF) 4G/PABP/poly(A) tail interaction is achieved instead through the

147 These guanosines frequently divide poly(A) tails into interspersed A-tracts and therefore c

148 A 3' poly(A) tail is a common feature of picornavirus RNA gen

149 e primers are removed by exonuclease I and a poly(A) tail is added to the 3' end of the first-strand

150 The foreshortened poly(A) tail is maintained by poly(A) ribonuclease, whic

151 sequence motifs that signal the addition of poly(A) tails is essential to improved genome annotation

152 One challenge in studying poly(A) tails is that they are difficult to sequence and

153 s tend to possess shorter poly(A)-tails, the poly(A)-tail is not required for the codon-mediated mRNA

154 s are polyadenylated in the nucleus, and the poly(A)-tail is required for efficient mRNA export and t

155 cDNA synthesis specifically toward the 3UTR/poly(A) tail junction of cellular RNA.

156 The alterations in poly(A) tail length accompanying elevated PDE12 expressi

157 In summary, we show that poly(A) tail length and 3' terminal uridylation have ess

158 oly(A) tail length and expression level, and poly(A) tail length and 3'-UTR length.

159 r the rhythmic mRNAs by their peak phases in poly(A) tail length and abundance of the long-tailed sub

160 of previously reported correlations between poly(A) tail length and expression level, and poly(A) ta

161 ar PAPS isoforms control de novo synthesized poly(A) tail length and hence expression of specific sub

162 strongest contributor to the rhythmicity in poly(A) tail length and the rhythmicity in the abundance

163 a function for Hnrnpa1 in the regulation of poly(A) tail length and translation of maternal mRNAs th

164 In fact, changes in poly(A) tail length are not sufficient to account for PN

165 n of pgc is concurrent with extension of its poly(A) tail length but appears largely independent of t

166 NA show growth defects as well as defects in poly(A) tail length but do not accumulate poly(A) RNA in

167 onsequences of altered pre-mRNA splicing and poly(A) tail length control.

168 a suggest that NPM1 has an important role in poly(A) tail length determination and may help network 3

169 st, highly reproducible and minor changes in poly(A) tail length distribution are easily detected.

170 Therefore, even though the poly(A) tail length dynamics seen between genotypes may

171 assays have enabled a more detailed look at poly(A) tail length genome-wide throughout many developm

172 cial for gene expression and perturbation of poly(A) tail length has been linked to a human neurodege

173 help of these advances, our understanding of poly(A) tail length has evolved over the past 5 years wi

174 etic data indicate that dNab2 restricts bulk poly(A) tail length in vivo, suggesting that this functi

175 on in the heart, where controlled changes in poly(A) tail length influence mRNA translation.

176 mportantly, we found that the rhythmicity in poly(A) tail length is closely correlated with rhythmic

177 Poly(A) tail length is emerging as an important marker o

178 also needed during oogenesis to regulate the poly(A) tail length of dmos during oocyte maturation and

179 ial action of RNA binding proteins modulates poly(A) tail length of maternal mRNAs, leading to asymme

180 A)denylome" analysis, a method that measures poly(A) tail length of transcripts in a global manner, a

181 se transcripts do not exhibit rhythmicity in poly(A) tail length or steady-state mRNA level, despite

182 basis for understanding its function in both poly(A) tail length regulation and in the compaction of

183 ly(A) RNA binding protein, Nab2, facilitates poly(A) tail length regulation together with targeting t

184 mRNP organization and compaction as well as poly(A) tail length regulation.

185 This poly(A) tail length restriction is controlled by Mtr4p.

186 d translation, and enzymes that regulate the poly(A) tail length significantly impact protein profile

187 me, consistent with previous reports linking poly(A) tail length with nuclear RNA surveillance.

188 f genes were found to exhibit rhythmicity in poly(A) tail length, and the poly(A) rhythms are strongl

189 We describe strategies for assessing 3' poly(A) tail length, base modifications and transcript h

190 Depletion of GLD4 not only reduced GLUT1 poly(A) tail length, but also GLUT1 protein.

191 define the function of PABPN1 in control of poly(A) tail length, little is known about the role of P

192 r-expression enhances AVP mRNA abundance and poly(A) tail length.

193 r PNPase nor RNase II has any effect on tRNA poly(A) tail length.

194 ing to RNA is critical for proper control of poly(A) tail length.

195 nal mRNAs is regulated by dynamic changes in poly(A) tail length.

196 that modulates both mRNA nuclear export and poly(A) tail length.

197 acterization of ZC3H14 as a regulator of RNA poly(A) tail length.

198 es, splicing events, poly(A) site choice and poly(A) tail length.

199 Furthermore, although poly(A)-tail length has been considered critical in post

200 Here we describe poly(A)-tail length profiling by sequencing (PAL-seq) an

201 During egg activation, relative changes in poly(A)-tail length, and thus translational efficiency,

202 When subjected to poly(A) tail-length assays, mitochondrial mRNAs from aff

203 We also determined that poly(A) tail lengths of transcripts vary across developm

204 Poly(A) tail lengths were similar for target mRNAs on po

205 RNAs, but difficulties in globally measuring poly(A)-tail lengths have impeded greater understanding

206 miRNAs have almost no effect on steady-state poly(A)-tail lengths of their targets in mouse fibroblas

207 , we profiled translational efficiencies and poly(A)-tail lengths throughout Drosophila oocyte matura

208 Poly(A)-tail lengths were conserved between orthologous

209 cleus with diverse intragenic and intergenic poly(A)-tail lengths.

210 nuclear-retained noncoding RNA with a short poly(A) tail-like moiety and a small tRNA-like cytoplasm

211 cay is generally initiated by removal of the poly(A) tail mediated by the Ccr4-Caf1-Not complex.

212 ng, and therefore reduced canonical (cap-and-poly(A)-tail-mediated) translation, remains undiscovered

213 ve levels of A17-PABPN1 and detected shorter poly(A) tails, modest changes in poly(A) signal (PAS) us

214 A)-binding proteins (PABs) uniformly bind to poly(A)-tailed mRNAs, regulating their stability and tra

215 cytoplasm they contribute to translation of poly(A)-tailed mRNAs.

216 these results indicate that, in addition to poly(A) tails, Nab2 can also recognize sequence motifs e

217 These results establish that neither the poly(A) tail nor PAB1 is required in yeast for discrimin

218 space between the termination codon and the poly(A) tail nor the binding of steady-state, largely hy

219 higher levels of expression by targeting the poly (A) tails of mRNAs.

220 The expansion, which occurs in the poly(A) tail of an AluSx3 element and differs in both si

221 tic cells, the shortening and removal of the poly(A) tail of cytoplasmic mRNA by deadenylase enzymes

222 While the 5' cap modification and the poly(A) tail of eukaryotic mRNA play key roles in regula

223 al protein, many copies of which bind to the poly(A) tail of eukaryotic mRNAs to promote translation

224 GLD-2-GLD-3 acts by extending the short poly(A) tail of germ-line-specific mRNAs, switching them

225 ng a reverse primer that hybridizes with the poly(A) tail of HIV-1 mRNAs, anchored by conserved viral

226 roximately 25% of the expressed genes have a poly(A) tail of less than 30 residues in a significant p

227 The 3' poly(A) tail of messenger RNA is fundamental to regulati

228 Proteins bound to the poly(A) tail of mRNA transcripts, called poly(A)-binding

229 PABP is able to bind the poly(A) tail of mRNA, as well as translation initiation

230 d this is accompanied by extension of the 3' poly(A) tail of the AVP mRNA, and the up-regulation of t

231 well as synthesize 5' methylated cap and 3' poly(A) tail of the transcribed viral mRNAs.

232 mitochondrial mRNAs, although the length of poly(A) tails of mitochondrial transcripts were unaffect

233 lar structures may be formed by the cellular poly(A) tails on mRNA.

234 c templates ensures the synthesis of long 3' poly(A) tails on progeny RNA genomes.

235 transferase reactions, leads to synthesis of poly(A) tails on the 3' end of VSV mRNAs that are 10- or

236 polymerase (PAP) catalyzes the synthesis of poly(A) tails on the 3'-end of pre-mRNA.

237 eir 3' untranslated regions (UTRs) have long poly(A) tails; once the RNAs are spliced and transported

238 s the enzyme responsible for the addition of poly(A) tails onto RNA molecules in Escherichia coli.

239 , including mRNAs, for degradation by adding poly(A) tails onto their 3' ends, these data indicate th

240 The longest NQO1 transcript has increased poly(A) tail (PA-tail) length that accounts for the diff

241 mination in vitro However, after binding the poly(A) tail, PABP became insensitive to suppression by

242 ssenger RNA function is controlled by the 3' poly(A) tail (PAT) and poly(A)-binding protein (PABP).

243 LARP4 was shown to promote poly(A) tail (PAT) lengthening and stabilization of indi

244 remain untranslated, whereas elongating the poly(A) tail permits protein production.

245 In the cytoplasm, poly(A) tails play pivotal roles in the translation and

246 The poly(A) tail protects the mRNA from unregulated degradat

247 largely to those obtained from conventional poly-A tail purification methods, indicating both enumer

248 e show that KPAF5 stabilizes KPAF4 to enable poly(A) tail recognition, which likely leads to mRNA sta

249 ssembly in vivo, suggesting new functions of poly(A) tail regulation in RNP dynamics.

250 ession and accelerated decay caused by rapid poly(A) tail removal [3, 5-12].

251 a two-color assay to simultaneously monitor poly(A) tail removal from different RNAs, we demonstrate

252 RNA expression posttranscriptionally through poly(A) tail removal.

253 encountered, translation continues into the poly(A) tail, resulting in C-terminal appendage of a pol

254 are uncoupled from transcription and exhibit poly(A) tail rhythms even though the steady-state mRNA l

255 transcribed rhythmically but do not exhibit poly(A) tail rhythms.

256 ondrial ribosome profiling and mitochondrial poly(A)-tail RNA sequencing (MPAT-Seq) assay, we identif

257 Whereas the poly(A) tail serves to provide such a tag at the 3' end

258 NA (mRNA) degradation, which is initiated by poly(A) tail shortening (deadenylation).

259 tent with this, Pum-Brat repression leads to poly(A) tail shortening and mRNA degradation in tissue c

260 This activity was associated with poly(A) tail shortening and regulated by heterogeneous n

261 Poly(A) tail shortening, also termed deadenylation, is t

262 Deadenylation, also called poly(A) tail shortening, is the first rate-limiting step

263 Deadenylation, also called poly(A) tail shortening, is the first, rate-limiting ste

264 F)1 in the CCR4-NOT complex function in mRNA poly(A) tail shortening.

265 yadenylation, which indicated that selective poly(A)-tail shortening primarily specifies these change

266 slation on transcripts containing or lacking poly(A) tails, suggesting that cleavage of PABP and IRES

267 Premature termination of poly(A) tail synthesis in the presence of cordycepin abr

268 Activation requires shortened/no poly(A)-tail targets; polyadenylated mRNAs are partially

269 Shortening of the poly(A) tail, termed deadenylation, reduces transcript s

270 termined that eIF4A2 bound mRNAs have longer poly(A) tails than DDX6 bound mRNAs.

271 re is a >30-fold increase of PAP I-dependent poly(A) tails that are </=10 nt in length coupled with a

272 translationally repressed mRNAs contain long poly(A) tails that are dramatically shortened during the

273 ventional poly(A) polymerase, which produces poly(A) tails that stabilize RNAs.

274 destabilizing codons tend to possess shorter poly(A)-tails, the poly(A)-tail is not required for the

275 (NOT)," which catalyzes the removal of mRNA poly-(A) tails, the first obligatory step in mRNA decay.

276 day-dependent oscillation for the Fabp7 mRNA poly(A) tail throughout murine brain.

277 t decapping is preceded by shortening of the poly(A) tail to a length that can no longer support tran

278 Polyadenylation is the addition of a poly(A) tail to an RNA molecule.

279 elements are linked to the propensity of the poly(A) tail to engage in double-stranded structures.

280 mRNA polyadenylation, the addition of a poly(A) tail to the 3'-end of pre-mRNA, is a process cri

281 ble miRNA-binding sites by looping the 3'UTR poly(A) tail to the bound miRISC and deadenylase.

282 The addition of the poly(A) tail to the ends of eukaryotic mRNAs is catalyze

283 The relationship of the poly(A) tail to translational control is intimately rela

284 ic acid (LNA)-containing oligo(dT) probes to poly(A) tails to maximize RNA capture selectivity and ef

285 The Ccr4-Not complex removes mRNA poly(A) tails to regulate eukaryotic mRNA stability and

286 ter trimming, amplification primer trimming, poly-A tail trimming, vector screening and low quality r

287 Third, the poly(A) tail was necessary for maximal PUM repression.

288 polymerase, and the mRNA transcribed, with a poly(A) tail, was efficiently utilized in an in vitro tr

289 where the majority of the PAP I synthesized poly(A) tails were after the Rho-independent transcripti

290 regulated by RNAi in Chlamydomonas, very few poly(A) tails were detected in chloroplasts for the atpB

291 the number or sequence of mitochondrial mRNA poly(A) tails, where unexpectedly we found, in addition

292 Analysis of poly(A) tails, which destabilize chloroplast RNAs, indic

293 cleases for pre-tRNA substrates adding short poly(A) tails, which not only modulate the stability of

294 EMT/metastasis-related mRNAs to have shorter poly(A) tails while other mRNAs to have longer poly(A) t

295 Orb2 represses translation and removes mRNA poly(A) tails, while the oligomeric form enhances transl

296 turely translated: a transcript with a short poly(A) tail will remain untranslated, whereas elongatin

297 on, we complexed synthetic mRNA containing a poly A tail with PABPs in a stoichiometric manner and st

298 nstrate that our tool can precisely identify poly(A) tails with near perfect accuracy at the speed re

299 onfirm that cordycepin reduces the length of poly(A) tails, with some mRNAs being much more sensitive

300 Mature MALAT1 thus lacks a poly(A) tail yet is expressed at a level higher than man