ライフサイエンスコーパス: poly A

1 If 0.01% poly A were included, the value of kcat/Km increased to

2 chromosomal loci (17%) showed TGF-beta 1RII poly A tract mutation, including 2 cancers and 1 dysplas

3 iral mRNAs that lack a 5' m7GTP cap and a 3' poly-A tail rely on structural elements in their untrans

4 canonical 5' 7-methylguanosine cap and a 3' poly-A tail.

5 ranscription can initiate upstream of the 3' poly-A tail during retrotransposon integration.

6 arise given that ribosomal RNA lacks the 3' poly-A tail typically associated with messenger RNA.

7 and eight ncRNAs contained non-templated 3'-poly-A or poly-AG additions.

8 on, we complexed synthetic mRNA containing a poly A tail with PABPs in a stoichiometric manner and st

9 PACS is not dependent on the existence of a poly A tail on an mRNA, it should have application to id

10 a typical polyadenylation signal preceding a poly A tail and the 5' UTR which includes 63-71 bp and t

11 CAI2 is a single-exon gene with a poly A signal located in but independent of the p16/ARF

12 s both a putative start of translation and a poly-A tail.

13 ntly monoallelic and the majority occur at a poly-A tract.

14 NA fragments with zinc finger homology had a poly-A tail and 3 of these fragments contained a putativ

15 245 sequence tags of a poly-A primed cDNA library and 55 sequence tags from a 1

16 The pathogenetic mechanism by which a poly-A tract results in these various human disorders re

17 , their 3' termini lack a polyadenylic acid (poly A) tail and instead contain 2-4 copies of a unique

18 Polyadenylic-polyuridylic acid (poly A:U) is a dsRNA mimetic explored empirically in can

19 Two tandem poly-adenylation (poly A) signals for the GHBP mRNA were found in the 3'-u

20 d there may be a choice of poly-adenylation (poly-A) signal sequence.

21 There are three alternative poly-A addition sites in the long 3' UTR and also six po

22 minal polyadenosine nucleotides (poly A) and poly A binding proteins (PABPs) for optimal expression,

23 d of interlaced networks of both poly A+ and poly A- annotated transcripts and unannotated transcript

24 imited to simple sequences like poly-I:C and poly-A:U.

25 rosslinking of the protein-RNA complexes and poly-A pull down are reported to contribute to biases an

26 expression profiles from rRNA-depletion and poly-A enrichment are similar.

27 exes consisting of eIF4E, eIF4G, eIF4A1, and poly-A binding protein.

28 se (PNPASE), a 3' --> 5' exoribonuclease and poly-A polymerase, in the mitochondrial intermembrane sp

29 riments, adding unique molecular indexes and poly-A tails to produce finalized probes, in situ probe

30 uencing of small RNAs, including miRNAs, and poly-A-enriched mRNAs.

31 LR ignores known SNPs, low quality reads and poly-A/T sequences.

32 sis of DNA sequences around splice sites and poly-A signals is able to explain several observations i

33 ng strongly in vitro to synthetic poly-U and poly-A oligoribonucleotides.

34 deling, conformational changes of poly-U and poly-A RNA are characterized as they interact with polyb

35 hanced the ability of ASL(Lys3)(UUU) to bind poly-A programmed ribosomes.

36 ntly composed of interlaced networks of both poly A+ and poly A- annotated transcripts and unannotate

37 erkes: an insertion consisting of a 40-70 bp poly-A and an 11 bp duplication of the exonic region pre

38 y-T tracts and precisely at their 3' ends by poly-A tracts.

39 assively-parallel, rapid approach to capture poly-A-rich genomic fractions within short 80-150bp ampl

40 ase chain reaction analysis of human cardiac poly A+ mRNA.

41 st some of the mRNA of P. aerophilum carried poly-A tails facilitating the construction of a cDNA lib

42 largely to those obtained from conventional poly-A tail purification methods, indicating both enumer

43 ite scan (PASS) has been developed to detect poly-A sites in the genome.

44 Mitochondrial DNA, poly A RNA, and the cytosolic nucleotide pool were the p

45 al RNA to yield sufficient mRNA using either poly-A selection or depletion of rRNA.

46 scription factor Arx when it has an expanded poly-A tract (Arx(E)), a mutation associated with infant

47 oly-T primers was copying rRNA with extended poly-A 3' ends.

48 o a nuclear region devoid of splicing factor/poly A RNA rich domains.

49 fibroblast growth factor receptor-1 (FGFR1), poly-A-binding protein, cAbl, heterogeneous nuclear ribo

50 somes, as well as increased its affinity for poly-A programmed ribosomes to the level of native Esche

51 This is a distinct function for poly-A tails, which are otherwise known primarily as sta

52 Our data demonstrate a novel mechanism for poly-A expansion diseases: the misregulation of a subset

53 rphic Alus, TypeTE identifies the hallmarks (poly-A tail and target site duplication) and orientation

54 s and occur preferentially in homopolymeric (poly A or poly T) genomic stretches.

55 The RET vector uses an improved poly A-trap strategy for the efficient identification of

56 ng (PolyA-seq) did not detect differences in poly A sites after THZ1 treatment.

57 ng system that is not dependent on an intact poly-A tail and showed that it could be used to analyze

58 ll as the vast majority of species that lack poly-A tails in their mRNAs (including all archea and ba

59 in histone transcripts which typically lack poly-A tails.

60 nd degradation, and that maintaining longer, poly-A-containing arms flanking the miRNA stem-loop mark

61 capture of information distant from the mRNA poly-A tail.

62 ned canine beta(3)-AR cDNA probe and myocyte poly A(+) RNA, we detected a single band about 3.4 kb in

63 ble for identifying interaction sites on non poly-A RNAs.

64 ption is adaptable to other large-scale, non-poly-A depleted, RNA-seq studies.

65 Here, we present novel poly A trap vectors that overcome the effect of NMD and

66 pair DNA hairpin attached to a 50-nucleotide poly-A tail (HP-A(50)) is threaded into an alphaHL chann

67 NA on 3'-terminal polyadenosine nucleotides (poly A) and poly A binding proteins (PABPs) for optimal

68 One single peritumoral dose of poly A:U was sufficient to induce IFN-beta, readily visu

69 ome and accumulation of Pol II downstream of poly A sites indicating increased levels of initiation a

70 e/uracil does not affect the fluorescence of poly A nanocapsules.

71 response elicited in a therapeutic model of poly A:U administration.

72 233-129,263 sites using as little as 2 ng of poly A(+) RNA.

73 PCR revealed 2.7 x 10(5) molecules per ng of poly A+ RNA.

74 on wild-type virus either at or upstream of poly A signals, depending on the gene.

75 ation of membraneless organelles composed of poly-A RNA and proteins.

76 nsposition events and subsequent mutation of poly-A microsatellites within L1.

77 ell transcriptome without the requirement of poly-A pulldown.

78 transcriptome data obtained by sequencing of poly-A RNA derived from postmortem dorsolateral prefront

79 iation is enhanced, positional order of only poly-A RNA is detected within phase separated RNP mixtur

80 Polyanionic polymers such as heparin or poly A substituted for NaCl.

81 a 3' hydroxyl group for adaptor ligation or poly-A tailing.

82 fferences in the ORF62 gene and in the ORF64 poly-A region successfully distinguished the Oka vaccine

83 how its proteins act to retrotranspose other poly A elements and the extent of its role in shaping th

84 Moreover, the results show that the PDEbeta poly-A signal has a dominant inhibitory effect over two

85 In each study cohort, we performed poly-A RNA-sequencing in baseline samples from 25 respon

86 These polyadenylation (poly A) trap vectors employ a splice donor to capture an

87 Polyalanine (poly-A) tracts exist in 494 annotated proteins; to date,

88 complexes (RNCs) with different polyalanine (poly-A) inserts or signal peptides from membrane/secreto

89 GF-beta 1RII coding region polydeoxyadenine (poly A) microsatellite tract was polymerase chain reacti

90 erence RNA standards to test four protocols (poly-A-selected, ribo-depleted, size-selected and degrad

91 3 more at the amino terminus, and a putative poly A tail.

92 itive sequences, such as poly-K/R, poly-N/Q, poly-A, and poly-G residues.

93 cating that TLR3 is required for recognizing poly A:U.

94 amplified from barley (Hordeum vulgare) root poly A+ RNA and used as probes to screen a barley root c

95 enylate StCycA2 mRNA, resulting in shortened poly-A tails of StCycA2 mRNA and subsequently reduced tr

96 different library preparation kits (standard poly-A versus total RNA with Ribozero depletion) and ana

97 We reveal that poly-A tails added to rRNA molecules are integrated into

98 Mutational inactivation of the poly A microsatellite tract within TGF-beta 1RII occurs

99 olorectal cancer patients is mutation of the poly A tract of the transforming growth factor-beta rece

100 rat and mouse GHR/GP gene suggests that the poly A signals and GT repeat may be involved in the regu

101 d non-coding RNAs lacking the 5' cap and the poly-A tail.

102 ing an epitope-tagged protein that binds the poly-A tail of mRNAs (FLAG::PAB-1) from an intestine-spe

103 the indirect interaction is mediated by the poly-A RNA-binding protein PABPC1.

104 single polyadenylation site 229 nt from the poly-A tail.

105 We identified the poly-A binding RBP, CPEB1, as a potential new regulator

106 d by the addition of peptides and, as in the poly-A system, these motifs exhibit positional ordering.

107 UAG stop codon immediately downstream of the poly-A tract.

108 plication of the exonic region preceding the poly-A (XM_548088.6:c.2110_2111ins[A(40_70);2100_2110])

109 A molecules is accomplished by targeting the poly-A tail with an oligo-dT20 modified gold nanoparticl

110 mmunoblotting, we were able to show that the poly-A inserts embedded in the passage tunnel can form a

111 main (ASL(Lys3)(UUU)), are unable to bind to poly-A programmed ribosomes.

112 We thus expect that a translated poly-A tail, encoding for positively charged lysines reg

113 ter trimming, amplification primer trimming, poly-A tail trimming, vector screening and low quality r

114 available methods of mRNA enrichment (TruSeq poly-A enrichment and RiboMinus rRNA depletion).

115 both these changes and increases in tubulin poly A+ mRNA and protein coexist indefinitely after a ne

116 tumor-specific immune response elicited upon poly A:U administration.

117 Total RNA was poly-A selected and RNA was sequenced to evaluate transc

118 t mammalian Larp1 is found in a complex with poly A binding protein and eukaryote initiation factor 4

119 d with probes to TIMP-1 to -4 and GAPDH with poly A+ mRNA from ventricular tissues of patients with i

120 s oligomer was similar to that observed with poly A or high salt concentration when the molar ratio o

121 ARP1) is a conserved RBP that interacts with poly-A-binding protein and is known to regulate 5'-termi

122 e for adoption of ultra-deep RNA-seq without poly-A selection to interrogate both linear and circular