ライフサイエンスコーパス: polylysine

1 red on fibronectin than in cells cultured on polylysine.

2 s by recombinant human CKII is stimulated by polylysine.

3 lylysine and 0.22 +/- 0.12% for [99mTc] DTPA-polylysine.

4 bronectin but had no effect on attachment to polylysine.

5 A-man-PL and 0.13 +/- 0.08% for [99mTc] DTPA-polylysine.

6 asing protein (BPI or CAP57), protamine, and polylysine.

7 consisting of asialoglycoprotein coupled to polylysine.

8 r the MARCKS peptide or with the addition of polylysine.

9 y degraded protein, it bound to MDA-modified polylysine.

10 polyelectrolyte complex made of alginate and polylysine.

11 nder an electric field or in the presence of polylysine.

12 nk formation is observed with either AP-1 or polylysine.

13 approximately 15.0 MBq (99m)Tc-DTPA-succinyl-polylysine (2 microg; DTPA is diethylenetriaminepentaace

14 wly formulated alginate-glutamine degradable polylysine (A-GD-PLL) microcapsules.

15 aximal stimulation occurs at 10 microg/ml of polylysine, a concentration which has no effect on prote

16 report the crystallographic structure of the polylysine-actin-latrunculin A complex at 3.5-A resoluti

17 was 2.84 +/- 0.83% for [99mTc]DTPA-mannosyl-polylysine and 0.22 +/- 0.12% for [99mTc] DTPA-polylysin

18 epared with either short chain or long chain polylysine and a beta-galactosidase expression plasmid.

19 nificant increase in nonspecific adhesion to polylysine and BSA.

20 Exogenous cationic polymers, polylysine and chitosan, can impart stimulatory activity

21 mplexes were formed between the mannosylated polylysine and oligonucleotides and added to the cells i

22 tions between the charged surface-associated polylysine and phosphatidylserine are sufficient to both

23 In contrast, polycations (polylysine and polyethylenimine) and low-molecular-weigh

24 bosylation of not only polyarginine but also polylysine and polyhistidine, and ribosylation was inhib

25 hod uses inexpensive, commercially available polylysine and reusable glass plates.

26 ation of coacervate microdroplets comprising polylysine and short single strands of DNA generates mem

27 ediation of the DHP receptor (e.g. caffeine, polylysine, and peptide A), induced Ca(2+) release witho

28 kinetics of inhibition by the PIP2 scavenger polylysine, and the inhibition by the phosphatidylinosit

29 ed by cross-linking polyethylene glycol with polylysine around a salt-leached polylactic-co-glycolic

30 of human serum albumin, gamma-globulins, and polylysine, as well as using media mimicking the extrace

31 tor, LPDII, where DNA was first complexed to polylysine at a ratio of 1:0.75 (w/w) and then entrapped

32 rowth cones also caused actin bundle loss in polylysine-attached growth cones without loss of actin m

33 lent presentation of CGP77175A on a modified polylysine backbone (degree of polymerization = 1200; 50

34 th a peptide mimetope of dsDNA on a branched polylysine backbone (DWEYSVWLSN-MAP) induces a systemic

35 anocarriers (H-Dots) are composed of epsilon-polylysine backbone for charge variations, near-infrared

36 A3 or A6 peptides constructed on a branching polylysine backbone.

37 ions using two distinct peptides on branched polylysine backbones (multiple Ag peptide)-peptides.

38 nanoparticle delivery platform (DEP)-SN38, a polylysine-based nanoparticle conjugate of the irinoteca

39 sed binding, none of the amines that inhibit polylysine binding inhibits PC binding, suggesting that

40 Polylysine bound permanently to RG-II, precluding electr

41 ine, spermine, tRNA, DNA, polyglutamic acid, polylysine, bovine serum albumin, or histone did not rel

42 nic polyamino acids such as polyarginine and polylysine by fluorescence polarization.

43 s responsible for reversed galvanotropism on polylysine by growing neurons on tissue culture dishes w

44 ferences in electrostatic attraction between polylysine, carrageenan, and pectin.

45 Polylysine-pectin or polylysine-carrageenan complexes were formed at mass rat

46 t high mass ratios, whereas those containing polylysine-carrageenan complexes were turbid and unstabl

47 Pectin did not strongly interact with polylysine-carrageenan complexes, whereas carrageenan di

48 (degree of polymerization = 1200; 50% of the polylysines carry the inhibitor) greatly enhanced in viv

49 the Ad type 7 fiber and retard DNA through a polylysine chain present at the C-terminus of this linke

50 have very high molecular weight (> 300,000) polylysine chains attached to them.

51 then fluorescently stained and stretched on polylysine-coated glass slides so that the positions of

52 The resulting cDNAs were arrayed on polylysine-coated glass slides.

53 fibronectin, laminin, or vitronectin but not polylysine-coated substrates.

54 ronectin but not to fibronectin, laminin, or polylysine-coated surfaces.

55 refore, we synthesized an ultrathin alginate-polylysine coating to display aptamers on the surface of

56 diated endocytosis of a ribozyme-transferrin-polylysine complex, specifically reduces both cellular F

57 in between those of the free DNA and the DNA.polylysine complex.

58 nd anti-IV in the form of asialoglycoprotein-polylysine complexes were administered to Huh7 cells, an

59 uptake showed a parallel relationship in the polylysine concentration range of 0-10 microM.

60 Under these conditions, the increase in the polylysine concentration resulted in the increase of bot

61 anodal turning were graded over the range of polylysine concentrations (0 = 0.1 < 1 < 10 = 100 microg

62 considered by growing neurites on a range of polylysine concentrations.

63 The polylysine conjugate was also longer acting than the mon

64 genes were complexed with asialoglycoprotein-polylysine conjugates, and 1 mg of the complexed DNA was

65 ine enkephalin, bradykinin, substance P, and polylysine-containing particles were determined with att

66 Dendrimers were synthesized on a polylysine core and bore either native GnRH (1, 2, and 5

67 a mixture of cytochrome b5, cytochrome c and polylysine, cytochrome b5 undergoes reversible electroch

68 The synthesis of a polylysine dendron containing eight GdDOTA units conjuga

69 ide-modified alpha-ImI to an alkyne-modified polylysine dendron.

70 n techniques demonstrated that an adenovirus-polylysine-deoxyribonucleic acid (DNA) complex can be us

71 However, surprisingly, we found that epsilon-polylysine did not have a significant impact on lipid di

72 ervations that the cationic polymer, epsilon-polylysine, disrupts bacterial membranes with greater af

73 bstrates of gamma-PAK, such as histone 1 and polylysine, do not stimulate autophosphorylation or acti

74 hylenetriamine pentaacetic acid polymannosyl polylysine (DTPA-man-PL) was synthesized and tested for

75 In the presence of 1-5 mM Ca2+ (or 10 microM polylysine) ECaSt/PDI augmented the bovine pancreatic tr

76 antibody complexes and (99m)Tc-DTPA-succinyl-polylysine enabled in vivo visualization of very small a

77 epsilon-Polylysine (epsilon-PL) is a broad-spectrum antimicrobia

78 gements can be blocked by positively charged polylysine, further implicating electrostatic interactio

79 With the most promising polylysine glycopolymer PL84(mimHNK-1)45 the inhibitory

80 ducing the 125I-ModOxLDL retention; however, polylysine had no effect.

81 Both mucin and polylysine had pronounced influences on the particle siz

82 the presence of multiple Lys-Lys sequences, polylysines have tremendously enhanced affinity.

83 meric desorption rate k0 of polytyrosine and polylysine homopeptides from flat surfaces.

84 influenced by the cell type tested, although polylysine homopolymers demonstrate levels of internaliz

85 ected with other vectors by using adenovirus-polylysine in BAEC.

86 ed imidazole groups to the epsilon-amines of polylysine in varying mole ratios (73.5 mol % imidazole,

87 llapse, we cultured Helisoma growth cones on polylysine in which lamellipodial collapse was prevented

88 er levels of calcium loading (>150 nmol/mg), polylysine induced monophasic Ca2+ release curves (witho

89 that inhibits actin polymerization, arrests polylysine-induced nucleation at the level of an antipar

90 sing two different systems (C167PM actin and polylysine-induced polymerization of alpha-actin) show t

91 Polylysine inhibits elongation at both temperatures.

92 ortant implications for the incorporation of polylysine into food systems, particularly those contain

93 p a multifunctional nanoparticle composed of polylysine, iron oxide, and CpG (PIC) to increase tumor

94 Polylysine is a cationic biopolymer with a strong antimi

95 Polylysine is added to prevent the normal decompaction o

96 In addition, epsilon polylysine is loaded into elastic matrices during phase

97 A cationic polyamino acid, such as polylysine, is then added to the reaction mixture, where

98 GF2) that was first chemically conjugated to polylysine (K).

99 tinas were removed, dissociated, plated on a polylysine/laminin substrate, and maintained in vitro fo

100 or 8-day-old Long Evans rats and cultured on polylysine/laminin-coated coverslips in serum-free mediu

101 formation and subsequent cross-linking with polylysine led to an increase in injected MSC half-life

102 In addition, both lysine octapeptide and polylysine ligands were accessible for binding to hepari

103 In summary, a lectin-based intranasal polylysine-linked synthetic peptide vaccine was effectiv

104 by replacing its PHD with a polyhistidine or polylysine linker.

105 The polylysine modification and formation of ecotropic retro

106 ate that formation of amphotropic retrovirus polylysine molecular conjugates (aMMLV-PL) enhanced gene

107 Polylysine-molossin is a 31 amino acid synthetic peptide

108 This peptide, when added to polylysine-molossin/DNA complexes at an optimal w/w rati

109 The addition of cationic lipids to polylysine-molossin/DNA conjugates gave excellent report

110 complexes at an optimal w/w ratio of 5:1:2 (polylysine-molossin/DNA/fusogenic peptide) resulted in 2

111 r to macromolecular acceptors (ubiquitin and polylysine) more strongly than transfer to small-molecul

112 aptotagmin interactions mediated by the C2 B polylysine motif are required to attain full synaptotagm

113 Our analyses reveal that a polylysine motif at residues 189-192 confers an inhibito

114 This polylysine motif binds clathrin-adapter protein AP-2, ne

115 Thus, the polylysine motif functions after endocytosis and before

116 The synaptotagmin 1 C2A polylysine motif functions independently of calcium-medi

117 ular surfaces involved, namely the conserved polylysine motif in the C2B domain and Ca(2+)-binding al

118 and provide evidence that a highly conserved polylysine motif in this loop supports binding to negati

119 Finally, mutation of the polylysine motif inhibits the Ca2+-independent ability o

120 We have demonstrated that incorporation of a polylysine motif into the pIX ectodomain results in a si

121 Together, our results demonstrate that the polylysine motif is required for efficient Ca2+-independ

122 uptake was unchanged in the mutants, and the polylysine motif mutant synaptotagmin was able to rescue

123 Polylysine motif mutants exhibited an apparent decreased

124 tagmin I (syt) transgenes harboring specific polylysine motif mutations into flies otherwise lacking

125 ic transmission, contains a highly conserved polylysine motif necessary for function.

126 endent interactions mediated through the C2A polylysine motif of synaptotagmin 1 function to modulate

127 Addition of human beta2GP1, polylysine, native low-density lipoprotein, or apolipopr

128 Conventional processes to obtain branched polylysine need several steps and the addition of specif

129 Neither adhesion to polylysine nor thrombin-induced aggregation caused simil

130 Neither polylysine nor two control peptides containing scrambled

131 18 DTPA and 82 mannosyl groups attached to a polylysine of 100 units ([99mTc]DTPA18-man82-PL100) were

132 he adhesion of cells to an adsorbed layer of polylysine on glass plates, followed by hypotonic lysis

133 We therefore examined the effect of polylysine on the digestion of corn oil-in-water emulsio

134 f labeled DNA the surfaces were treated with polylysine or 3-aminopropyl triethoxysilane or were coat

135 y blocking PI4K or using the PIP2 scavengers polylysine or bovine serum albumin reduced the incidence

136 comprising poly(ethylene oxide) and either a polylysine or polyester dendron were prepared and hydrop

137 an irrelevant plasmid bound to mannosylated polylysine or the expression plasmid bound to galactosyl

138 The ability of beta2GP1, polylysine, or low-density lipoprotein to be a "cofactor

139 is triggered when polyphosphates bind to the polylysine patch in C2B domain and is stabilized by Mg(2

140 We examined the stability of polylysine-pectin complexes in the presence of carrageen

141 Solutions containing polylysine-pectin complexes were slightly turbid and rel

142 s, whereas carrageenan displaced pectin from polylysine-pectin complexes, which was attributed to dif

143 Polylysine-pectin or polylysine-carrageenan complexes we

144 PEG-PLL-Oleic acid, PPO), and GEM-conjugated polylysine (PEG-PLL-OA-GEM, PPOGEM), PPO/PPOGEM (POEM))

145 nanocarrier (consisting of lipid-derivatized polylysine (PEG-PLL-Oleic acid, PPO), and GEM-conjugated

146 either an integrin-binding RGD peptide or a polylysine peptide in the exposed HI loop.

147 an amide I band of ionized polyglutamate and polylysine, peptides which adopt a polyproline II helica

148 use the arginine-glycine-aspartame (RGD) and polylysine (pK7) motifs have been shown to enhance Ad5 i

149 at is condensed by electrostatic forces with polylysine (PL) covalently linked to streptavidin (binds

150 ximately 100 different complexes of DNA with polylysine (PL) or PL covalently attached to the glycopr

151 rus protein final sigma1, when conjugated to polylysine (PL), can bind the apical surface of M cells

152 mid DNA containing the gene of interest with polylysine (PL), PL linked to a replication-incompetent

153 The effects of polylysine (PLL) and PLL-asialoorosomucoid (AsOR) on DNA

154 ay, however, where M1-4 lanes alternate with polylysine-(Plys)-only lanes, RGC axons from goldfish, z

155 on of polyplexes composed of plasmid DNA and polylysine (POL), a non-buffering polyamine, or the stro

156 nt produced in yeast and covalently bound to polylysine [poly(K)] to enable binding of DNA.

157 Polycations such as polylysine, polyarginine, histone H1, histones H2A-H2B,

158 ng the ED(50) values of five macromolecules: polylysine, polyarginine, protamine, low-density lipopro

159 In contrast, less than 1 muM of a ChoP-polylysine polymer inhibited specifically the adhesion o

160 ltivalent nature of the MAG-IgM interaction, polylysine polymers of different sizes were substituted

161 bserved effects were highly dependent on the polylysine position in the mature domain.

162 ectrolytes consisting of cyanine dye pendant polylysines ranging in number of polymer repeat units (N

163 arboxyl-terminal methionine and the upstream polylysine region are important determinants for geranyl

164 cells confirmed the importance of the K-Ras polylysine region for microtubule binding, as deletion o

165 These studies show that the polylysine region of K-Ras is critical for its interacti

166 of Ha-Ras and vice versa, we found that the polylysine region of K-Ras located immediately upstream

167 ible polycations consisting of histidine and polylysine residues (HIS RPCs) were evaluated for their

168 e expression plasmid bound to galactosylated polylysine resulted in no detectable transgene expressio

169 rted by polycations (neomycin, spermine, and polylysine) reversing the effect of PIP2 on TNP-ATP bind

170 netics observed for precursors with multiple polylysine segments support a model in which translocati

171 farnesyl modification and positively charged polylysine sequence.

172 ing of either eight consecutive lysines or a polylysine sequence.

173 The primary epsilon-amine terminus of polylysine served as a model for the cationic centers, w

174 with polycation poly(ethylene glycol) (PEG)-polylysine (single-coat (SC) nanozyme), we introduce for

175 em viscosity are determined for concentrated polylysine solutions.

176 erol product formed in the reaction binds to polylysine SPA beads, producing a signal that is measure

177 Consequently, polylysine SPE/MALDI-MS targets offer a practical soluti

178 ing sequence of a variety of genes, encoding polylysine stretches that are important for protein func

179 l root ganglion (DRG) neurite outgrowth on a polylysine substrate and that asymmetric cGMP elevation

180 Adhesion of macrophages to a polylysine substrate had no effect.

181 which transfected neurons were replated onto polylysine substrates suggest that activation of MEK is

182 be achieved using the antisense mannosylated polylysine system.

183 oximal external region (MPER), extended by a polylysine tail.

184 Furthermore, these polylysine targets can capture over 60% of a protein fro

185 ration, for example, is some 50% larger with polylysine than with polyarginine.

186 lecular conjugate consisting of mannosylated polylysine that exploits endocytosis via the macrophage

187 ecular conjugate, consisting of mannosylated polylysine that exploits endocytosis via the macrophage

188 Ca2+ release from the SR was induced by polylysine (the ryanodine receptor-specific Ca2+ release

189 ography and wet etching and then coated with polylysine to facilitate cell adherence.

190 rylation does not occur when cells attach to polylysine, to which cells adhere in a nonspecific fashi

191 tail, resulting in C-terminal appendage of a polylysine tract and a terminally stalled ribosome.

192 The neurons are cultured on polylysine-treated coverslips, which are suspended above

193 ace membranes of heat-killed neurons than on polylysine, underlining the importance of plasma membran

194 e due to the fluorescent dye, do not bind to polylysine unless hybridized to the negatively charged D

195 g lysine octapeptide, Ad vector displaying a polylysine was capable of recognizing cellular heparan s

196 ked DNA nor DNA complexed with unsubstituted polylysine was effective in gene transfer.

197 , and even with HIV nucleocapsid protein and polylysine, was unacceptable; the exception was a form o

198 es with differential adhesion to immobilized polylysine, we show that neuronal colony-forming precurs

199 ing enthalpies (polyhistidine, polyarginine, polylysine), weighted by numbers of such cationic groups

200 Nucleoids released in the presence of polylysine were easily processed and provided well-defin

201 Adhesion to BSA and polylysine were not affected.

202 negatively charged, but neurites growing on polylysine, which is positively charged, turned toward t

203 ce with results obtained earlier for ionized polylysine, which suggest a high polyproline II propensi

204 f (111)In-diethylenetriaminepentaacetic acid-polylysine Z2D3-F(ab')(2), and SPECT imaging was perform