ライフサイエンスコーパス: polyribosome

1 asm by repressing the translation of mRNA at polyribosomes.

2 rred on resource-poor spines lacking SER and polyribosomes.

3 ch is incorporated into actively translating polyribosomes.

4 global protein synthesis and disassembly of polyribosomes.

5 tinal isoforms of IMPDH1 also associate with polyribosomes.

6 found to export and associate normally with polyribosomes.

7 tubule-bound mRNAs (MT-mRNAs) associate with polyribosomes.

8 ly promotes the association of this RNA with polyribosomes.

9 exported to the cytoplasm and is present in polyribosomes.

10 in addition to promoting ATX2 assembly with polyribosomes.

11 over, some CUTs appear to be associated with polyribosomes.

12 toplasmic 61-kDa species was associated with polyribosomes.

13 nical polyadenylation site, and associate to polyribosomes.

14 hat FMRP forms complexes with cortical brain polyribosomes.

15 both in nucleus, and in the cytoplasm on the polyribosomes.

16 sequence were preferentially associated with polyribosomes.

17 h appearance of specific mRNA transcripts in polyribosomes.

18 component of both soluble and membrane-bound polyribosomes.

19 Furthermore, Tap, but not NXT is detected in polyribosomes.

20 oprotein (mRNP) complex that associates with polyribosomes.

21 ibonucleoprotein particle is associated with polyribosomes.

22 d FMRP is associated with apparently stalled polyribosomes.

23 ng protein that is primarily associated with polyribosomes.

24 n a soluble form, as well as associated with polyribosomes.

25 llular localization of the luc transcript to polyribosomes.

26 edominantly cytoplasmic, and associates with polyribosomes.

27 inase C (PKC)-induced recruitment of mRNA to polyribosomes.

28 mic lysates is predominantly associated with polyribosomes.

29 gag RNA nuclear export and association with polyribosomes.

30 n of MBP mRNAs associated with membrane-free polyribosomes.

31 nt gamma-globin chains prior to release from polyribosomes.

32 cruit and also to maintain maternal mRNAs on polyribosomes.

33 tment, Cp mRNA was primarily associated with polyribosomes.

34 tion of numerous transcripts and subsynaptic polyribosomes.

35 small ribosome subunits, monoribosomes, and polyribosomes.

36 mRNP particles that are not associated with polyribosomes.

37 to an increase in the amount of p27 mRNA in polyribosomes.

38 pected for proteins that are associated with polyribosomes.

39 istributed with 80 S ribosomal particles and polyribosomes.

40 aining mRNA and promote its association with polyribosomes.

41 involving increased association of mRNA with polyribosomes.

42 aptic mRNAs are transported as stably paused polyribosomes.

43 RNase through interaction with the enzyme in polyribosomes.

44 genous Dhh1 accompanies slowly translocating polyribosomes.

45 and (3) Herc5 is physically associated with polyribosomes.

46 after LTP on resource-rich spines containing polyribosomes (4% larger than control) or SER (15% large

47 The dynamics of polyribosome abundance were studied in gravistimulated m

48 We previously found that polyribosomes accumulate in dendritic spines of the adul

49 clin A2 in adult neurons results in neuronal polyribosome aggregations and learning and memory defici

50 Here we optimize methods to analyze brain polyribosomes, allowing us to definitively demonstrate t

51 upled in vitro transcription/translation and polyribosome analysis demonstrated a strong association

52 Polyribosome analysis indicated that oxidized bases in m

53 for FMRP function, we examined their role in polyribosome and mRNA association.

54 specially on enlarged spines containing both polyribosomes and a spine apparatus.

55 grown under non-permissive conditions, lose polyribosomes and accumulate free 80S ribosomes.

56 s of evidence that JAKMIP1 is a component of polyribosomes and an RNP translational regulatory comple

57 his network is localized in dendrites, where polyribosomes and associated membranous elements are pos

58 ) Ferredoxin 1 (Fed-1) mRNA dissociates from polyribosomes and becomes destabilized when photosynthes

59 ts R521G and P525L associate abundantly with polyribosomes and decrease global protein synthesis.

60 le promoter results in formation of half-mer polyribosomes and decreased Qsr1p levels on free 60S sub

61 with RNase A caused a coincident collapse of polyribosomes and each Upf protein into fractions contai

62 quantitatively associated with mRNA, both in polyribosomes and in free mRNPs.

63 component, and found that FMRP moved out of polyribosomes and into SGs subsequent to oxidative stres

64 a large portion of the mRNA dissociates from polyribosomes and is subsequently degraded.

65 rotein-coding IFNL4 mRNA are not loaded onto polyribosomes and lack a strong polyadenylation signal,

66 We report that Bfr1p associates with yeast polyribosomes and mRNP complexes even in the absence of

67 There were more polyribosomes and multivesicular bodies throughout the d

68 , thereby reducing p53 mRNA association with polyribosomes and p53 translation.

69 its Drosophila homolog, ATX2, assemble with polyribosomes and poly(A)-binding protein (PABP), a key

70 erely reduced the association of TbAGO1 with polyribosomes and RNAi-induced cleavage of mRNA.

71 inal domain blocked association of AGO1 with polyribosomes and severely affected mRNA cleavage.

72 cantly depleted in organelle content such as polyribosomes and showed abnormal (vesiculated) mitochon

73 ng detected Fyn mRNA specifically in soluble polyribosomes and soluble Fyn protein was only detected

74 scharge subsided beyond 10 s, FMRP levels in polyribosomes and stress granules did not return to basa

75 Furthermore, coincident polyribosomes and synapse enlargement might indicate spi

76 we show that FUS can associate with stalled polyribosomes and that this association is sensitive to

77 er 30 min of gravistimulation, the levels of polyribosomes and the amount of polyribosome-associated

78 stingly, these complexes are associated with polyribosomes and the endoplasmic reticulum.

79 n translation as it is found associated with polyribosomes and the rough endoplasmic reticulum.

80 y nondescript but contain massive numbers of polyribosomes, and (5) labeled degenerating hair cells.

81 4 cosediments with 40S ribosome subunits and polyribosomes, and its knockdown decreases translation.

82 at permissive temperatures, slows run-off of polyribosomes, and reduces binding to eEF1A.

83 her MAEL nor piRNA precursors associate with polyribosomes, and they may arise from an imbalance betw

84 of TbAGO1 is that a fraction sediments with polyribosomes, and this association is facilitated by an

85 rs even when most of the mRNA is retained on polyribosomes, and thus is likely an independent event r

86 leaves is stabilized by its association with polyribosomes, and/or by translation.

87 he levels 40 S, 60 S, and 80 S monosomes and polyribosomes are unaffected by the loss of PRMT3, but t

88 in cycloheximide-treated cells is present on polyribosomes as a result of continued synthesis and tra

89 H domains and RGG boxes that associates with polyribosomes as a ribonucleoprotein particle.

90 ing protein in yeast known to associate with polyribosomes as an mRNP component, although its biologi

91 sociate with both soluble and membrane-bound polyribosomes as an mRNP component.

92 NA was able to displace FXR1P and FXR2P from polyribosomes as it does for FMRP, and this displacement

93 ing protein that associates with translating polyribosomes as part of a large messenger ribonucleopro

94 luding thylakoid membranes, carboxysomes and polyribosomes, as well as phages, inside the congested c

95 m knockout mice did not manifest accelerated polyribosome assembly or protein synthesis as it occurs

96 on, de novo protein synthesis, and dendritic polyribosome assembly.

97 in illuminated plants but in darkness is not polyribosome associated and is thus rapidly degraded by

98 ies detected by pCD41 are polyadenylated and polyribosome associated, suggesting that they may be act

99 miR-107 levels, leading to downregulation of polyribosome-associated MOR-1A in both Be(2)C cells and

100 he levels of polyribosomes and the amount of polyribosome-associated mRNA gradually increased over 24

101 s was accompanied by a transient decrease in polyribosome-associated mRNA.

102 nerve processes was used to screen synaptic polyribosome-associated mRNA.

103 ufficient to prevent complete translation of polyribosome-associated mRNAs.

104 FMRP is a polyribosome-associated neuronal RNA-binding protein, su

105 0 rapidly shifts existing TNFalpha mRNA from polyribosome-associated polysomes to monosomes.

106 Although FMRP has been characterized as a polyribosome-associated regulator of translation, less i

107 high-fat diet for 7 days showed increases in polyribosome-associated RNA and phosphorylation of S6K,

108 Longer incubations led to depletion of polyribosome-associated RNA, consistent with activation

109 FMRP is a polyribosome-associated RNA-binding protein that regulat

110 and plants, emerging evidence indicates that polyribosome-associated transcripts can be translational

111 expression of multiple genes, is one of the polyribosome-associated transcripts.

112 f function, but the mutant proteins remained polyribosome-associated.

113 ny of the events exhibiting isoform-specific polyribosome association are highly conserved across mam

114 nucleus and cytoplasm, and further implicate polyribosome association as an essential component of Sc

115 iguous segments of GCN1 that lead to reduced polyribosome association by GCN1.GCN20 in living cells w

116 RNA specifically undergoes markedly enhanced polyribosome association in the hippocampus in response

117 ur before the decline in mRNA abundance, and polyribosome association is rapidly reversible if plants

118 Furthermore, polyribosome association is severely compromised for bot

119 We find that differences in polyribosome association may be explained, at least in p

120 l inhibitors, does not inhibit the efficient polyribosome association of a Puf5p target mRNA.

121 ites in the 3'UTR have a strong influence on polyribosome association of alternative mRNA isoforms.

122 t nuclear pre-mRNA processing influences the polyribosome association of alternative mRNA isoforms.

123 isease-causing mutation, D226N, disrupts the polyribosome association of at least one retinal IMPDH1

124 ly responsive to light, we characterized the polyribosome association of these mRNAs in the light and

125 9G8 also enhanced polyribosome association of unspliced RNA containing a C

126 The Top3beta-polyribosome association requires TDRD3, which directly

127 A abundance are associated with increases in polyribosome association that require both a functional

128 es 533 and 538 were required for normal FMRP polyribosome association whereas all four arginines play

129 the same binding site on RNA mediating their polyribosome association, and that they may be functiona

130 titutions blocked the normal light effect on polyribosome association, whereas both altered dark-indu

131 in mouse erythroblasts and investigated mRNA polyribosome association, which revealed deregulated tra

132 led to a loss of siRNAs but did not abolish polyribosome association.

133 nce for the important role of the RGG box in polyribosome association.

134 and Levy first reported their observation of polyribosomes at the base of spines, the prevailing view

135 ation or to the presence of half-mers, i.e., polyribosomes awaiting a 60S subunit, our data show that

136 multaneously produced a greater reduction in polyribosome binding by GCN1.GCN20 and a stronger decrea

137 is enhanced phosphorylation of at least six polyribosome binding proteins.

138 Among the polyribosome bound proteins are the p90rsk-activating ki

139 gy and gene expression profiles of total and polyribosome-bound mRNA genome wide.

140 lts demonstrate that Scp160p associates with polyribosome-bound mRNP complexes in vivo, implicating a

141 hat unfolded protein response (UPR)-elicited polyribosome breakdown resulted in the continued associa

142 he initiation stage of protein synthesis and polyribosome breakdown.

143 ng that FMRP in brain is not associated with polyribosomes, but is part of small ribonucleo-protein c

144 ction increased c-jun/betaGal mRNA levels in polyribosomes by 2.3-fold, which indicates that translat

145 we have found that Scp160p is released from polyribosomes by EDTA in the form of a large complex of>

146 ytosol and comigrates with monoribosomes and polyribosomes by sucrose density gradient sedimentation.

147 ts suggest that the virion RNase is bound to polyribosomes by virtue of the reported association with

148 We further show that mRNA on polyribosomes can be targeted for degradation, although

149 ent and poly(A)- mRNA associate with smaller polyribosomes compared with cells with normal ribosome l

150 ions in AD and directly demonstrate that the polyribosome complex is adversely affected early in the

151 nstrate in neuroblastoma cells that the FMRP-polyribosome complexes are sensitive to puromycin, a dru

152 in enhanced recruitment of the HCV RNA into polyribosome complexes in vivo but only partially rescue

153 Accordingly, the HP RNA was retained within polyribosome complexes in vivo that were refractory to I

154 Similarly, polyribosome complexes remained stably positioned at spi

155 y of the Otx2(lambda) mRNA to form efficient polyribosome complexes was impaired.

156 nslocation of multifunction kinase p90rsk to polyribosomes; concomitantly, there is enhanced phosphor

157 onstrates that dFMRP does not associate with polyribosomes, consistent with their reported exclusion

158 ally, we find that IMPDH1 is associated with polyribosomes containing rhodopsin mRNA.

159 the majority of transcripts associated with polyribosomes decreased, resembling a general stress res

160 Analysis of liver polyribosomes demonstrated that the post-transcriptional

161 n of transcripts for vacuolar invertase with polyribosomes did not change over this period.

162 vivo but only partially rescued the RNA from polyribosome dissociation induced by IFN treatment.

163 et of the mRNAs showed dramatic dark-induced polyribosome dissociation, similar to Fed-1 mRNA, and al

164 and all of the mRNAs showed at least slight polyribosome dissociation.

165 red with eIF4GI depletion were excluded from polyribosomes due to the presence of multiple upstream o

166 Additional studies revealed that polyribosomes extracted from cytoplasm of wild-type viru

167 ly up-regulated and selectively recruited to polyribosomes following influenza virus infection.

168 y recruited maternal mRNAs were removed from polyribosomes following subsequent cleavage of eIF4G, in

169 ion of DHX33 protein levels markedly reduced polyribosome formation and caused the global inhibition

170 d RBV inhibit HCV IRES through prevention of polyribosome formation.

171 cDNA array analysis of the polyribosome fraction demonstrated that retinoic acid re

172 , the advantage of which is that hundreds of polyribosome fractionations can be performed simultaneou

173 significantly increased levels in the heavy polyribosome fractions following miRNA miR-30a-3p knockd

174 Upf2p shifted toward heavier polyribosome fractions in the absence of Upf1p and into

175 of each mutant protein into 30 S, 70 S, and polyribosome fractions in vivo.

176 nic stimulation, with a commensurate loss of polyribosomes from dendritic shafts at 2 hr posttetanus.

177 We isolated polyribosomes from Escherichia coli cells expressing GFP

178 Polyribosomes from fetal kidneys labeled in vitro with 1

179 termination and induces the dissociation of polyribosomes from mRNA.

180 rate incorporation of labeled amino acids by polyribosomes from neonatal kidneys declined sharply to

181 nism by which FUS affects the translation of polyribosomes has not been established.

182 Association of eEF2 with polyribosomes, however, was unchanged upon expression of

183 Utilizing RiboTag mice and polyribosome immunoprecipitation, we performed endotheli

184 xCR1 mRNA was associated with polyribosomes in animal cells but not vegetal cells.

185 ere they mediate mRNA translation as part of polyribosomes in animals.

186 etion transcript variant was associated with polyribosomes in cells.

187 scripts for HMGR-FLAG remain associated with polyribosomes in dark-treated tissues.

188 ectron microscopy reconstructions to examine polyribosomes in dendrites when memory formation was blo

189 We found that 4EGI-1 depleted polyribosomes in dendritic shafts and selectively preven

190 The presence of polyribosomes in dendritic spines suggests a potential i

191 naptic density formation and fewer dendritic polyribosomes in hippocampi of AMPKalpha2 cKO mice.

192 ively enriched in actively translating large polyribosomes in IL-4-pretreated cells compared with cel

193 relatively stable when it is associated with polyribosomes in illuminated plants but in darkness is n

194 Each of the MIE transcripts associates with polyribosomes in infected cells and therefore contribute

195 ctron microscopy reconstructions to quantify polyribosomes in LA dendrites when consolidation was blo

196 ndicate that FMRP associates with functional polyribosomes in neurons.

197 The association of FMRP with polyribosomes in non-neural cell lines has previously su

198 mber of RNAs show increased association with polyribosomes in PDK-1-/- ES cells relative to PDK-1+/+

199 erential association of ATF5 mRNA with large polyribosomes in response to stress.

200 d to fractions lighter than those containing polyribosomes in the absence of Upf2p.

201 ic spine bases, (3) endoplasmic reticula and polyribosomes in the cytoplasm of dendritic shafts, and

202 Electron microscopy showed ribosomes/polyribosomes in the distal parts of axon tips and in as

203 RNAs containing this element dissociate from polyribosomes in the leaves of transgenic tobacco (Nicot

204 found that Fed-1 transcripts accumulated on polyribosomes in the light but were found primarily in n

205 o alter the association of Tmprss6 mRNA with polyribosomes in the liver of rats indicating a lack of

206 profiles revealed a significant reduction in polyribosomes in torpid animals, indicating that transla

207 ocampus, FMRP shifted into SGs and away from polyribosomes, in both hippocampi.

208 The percentage of spines containing polyribosomes increased from 12% +/- 4% after control st

209 Analysis of translating polyribosomes indicated that hsp90 interacts with nascen

210 ation status and they remain associated with polyribosomes, indicating inhibition at a postinitiation

211 o attenuation of IL-12 mRNA association with polyribosomes inhibiting translation while IL-10 mRNA as

212 rate that the association of FMRP with brain polyribosomes is abrogated by competition with the FMRP

213 Histone mRNA from polyribosomes is immunoprecipitated with anti-SLBP.

214 cifically, we show that FUS association with polyribosomes is increased by Torin1 treatment or when c

215 The assembly of ATX2 with polyribosomes is mediated independently by two distinct

216 ethod for sedimentation velocity analysis of polyribosomes is presented that is based on low-speed ce

217 735A mutant bound siRNAs and associated with polyribosomes, it displayed a severe defect in the cleav

218 tion is initiated prematurely in perinuclear polyribosomes, leading to overproduction of receptors bu

219 elective RNA-binding protein associated with polyribosomes, leading to the hypothesis that FMRP may b

220 at FMRP interaction with RNA and translating polyribosomes leads to synapse loss.

221 The transient decrease in polyribosome levels was accompanied by a transient decre

222 rocess coupled to photosynthesis affects the polyribosome loading of a subset of cytoplasmic mRNAs.

223 RBV also blocked polyribosome loading of HCV-IRES mRNA through the inhibi

224 portant for Fed-1 mRNA stability rather than polyribosome loading.

225 nslation with a significant reduction in the polyribosome/monoribosome ratio for Pdx1 mRNA.

226 Polyribosomes, mRNA, and other elements of translational

227 data indicate that association of FMRP with polyribosomes must be functionally important and imply t

228 Although HAC1u mRNA is associated with polyribosomes, no detectable Hac1pu is produced unless t

229 agment lacking the ability to associate with polyribosomes normalized all observed excitability defec

230 We found that palmitate acutely increases polyribosome occupancy of total RNA, consistent with an

231 calreticulin and calmodulin transcripts into polyribosomes occurred predominantly in the lower pulvin

232 In parallel studies, mRNAs from polyribosomes of fragile X cells were used to probe micr

233 the interaction between TDP-43 and RACK1 on polyribosomes of neuroblastoma cells with mis-localizati

234 or the association of the AGO3 effector with polyribosomes or target transcripts.

235 Immunoaffinity purification of polyribosomes (polysomes) from crude leaf extracts of Ar

236 nation promoted adjustments in the levels of polyribosomes (polysomes) that were highly coordinated w

237 ere, we determined that CSP1 associates with polyribosomes (polysomes) via an RNA-mediated interactio

238 Polyribosome profile analysis confirmed that elevated ma

239 We sought to address this issue through polyribosome profile analysis of islets from mice fed 16

240 plasmic abundance, 251 mRNAs had an abnormal polyribosome profile in the absence of FMRP.

241 dom34delta mutants display an altered polyribosome profile that is rescued by expression of RP

242 There was no change in the shape of the polyribosome profile with increasing age, but before bir

243 on (TMA7), translation fidelity (TMA20), and polyribosome profiles (TMA7, TMA19, and TMA20).

244 In dbp2Delta cells, polyribosome profiles are deficient in free 60S subunits

245 cting shifts in individual mRNA abundance in polyribosome profiles following miRNA knockdown via siRN

246 had no effect on either the abundance or the polyribosome profiles of endogenous histone H1 or transg

247 s had little effect on cell growth or on the polyribosome profiles.

248 ation requires translation, we also examined polyribosome profiles.

249 by methionine incorporation and analysis of polyribosome profiles.

250 By polyribosome profiling of tumor tissues and human breast

251 We developed a brain polyribosome-programmed translation system, revealing th

252 ion of the RNAi machinery and target mRNA on polyribosomes promotes an efficient RNAi response.

253 d it is straightforward and does not require polyribosome purification or in vitro RNA footprinting.

254 protein synthesis, accumulation of half-mer polyribosomes, reduced levels of 60S ribosomal subunits

255 s and the appearance of new complexes in the polyribosome region of the gradient.

256 ults in increased loading of H2AFX mRNA onto polyribosomes, resulting in increased expression of H2A.

257 al ratio of ribosomes to mRNA as revealed by polyribosome sedimentation analysis.

258 Polyribosome sedimentation velocity centrifugation can b

259 stribution of key cellular resources such as polyribosomes, smooth endoplasmic reticulum, and synapti

260 rane system comprising the nuclear envelope, polyribosome-studded peripheral sheets, and a polygonal

261 ns of smooth endoplasmic reticulum (SER) and polyribosomes, subcellular resources important for synap

262 of interferon regulatory factor-1 mRNA with polyribosomes, suggesting iron was not essential for int

263 a 7.6-kilobase RNA that was associated with polyribosomes, suggesting it is translated.

264 ncorporation of c-fos mRNA into the heaviest polyribosomes, suggesting redox regulation of c-fos mRNA

265 en was associated with spine apparati and/or polyribosomes, suggesting that estrogen might act locall

266 IMPDH1) and IMPDH type 2 are associated with polyribosomes, suggesting that these housekeeping protei

267 RNA assembles into 60 S ribosomes which form polyribosomes, suggesting that they function in protein

268 rtion of dendritic spine synapses containing polyribosomes than did the cortices of wild-type mice, c

269 Repressed mRNAs are associated with polyribosomes that are engaged in translation elongation

270 This revealed two major pools of polyribosomes that were upregulated during memory format

271 e miRNAs that were tested cofractionate with polyribosomes, the sites of active translation.

272 r1p disrupts the interaction of Scp160p with polyribosomes, thereby demonstrating that the relationsh

273 Unlike normal polyribosomes, these complexes were resistant to breakdo

274 overall translation and that its binding to polyribosomes through RACK1 may promote, under condition

275 rotein upon synaptic signaling using stalled polyribosomes to bypass the rate-limiting step of transl

276 sed from stress granules and associates with polyribosomes to increase protein synthesis in a CAP-ind

277 a subtle yet significant shift from soluble polyribosomes to soluble mRNPs for at least two of these

278 min of gravistimulation, the amount of large polyribosomes transiently decreased.

279 s suggest that the association of Upf2p with polyribosomes typically found in a wild-type strain depe

280 pletion of PTC-containing mRNA isoforms from polyribosomes, underscoring the functional relationship

281 that normal FMRP associates with elongating polyribosomes via large mRNP particles.

282 ranslation while IL-10 mRNA association with polyribosomes was not affected.

283 calreticulin and calmodulin transcripts with polyribosomes was seen predominantly in the lower one-ha

284 ies showing that VHS-RNase degrades mRNAs in polyribosomes, we constructed a mutant in which NES was

285 Although many MT-mRNAs associate with polyribosomes, we find that active translation is not re

286 Polyribosomes were associated with the spine apparatus u

287 IIalpha) mRNA levels in actively translating polyribosomes were dysregulated in synaptoneurosomes fro

288 Postsynaptic densities on spines containing polyribosomes were larger after tetanic stimulation.

289 lated 9G8 was present in monosomes and small polyribosomes, whereas soluble fractions contained only

290 rilipin A mRNAs were efficiently loaded with polyribosomes whether or not fatty acids were added to t

291 proteins from several animals associate with polyribosomes, which are units of mRNA translation, wher

292 As containing 5' UTR GRSF-1 binding sites to polyribosomes, which is mediated through interactions wi

293 ed FMRP associated with actively translating polyribosomes while a fraction of phosphorylated FMRP is

294 n and greater association of the M mRNA with polyribosomes, while general translation was unaffected.

295 represent the quantal product of one or more polyribosomes, while inter-flash intervals appear random

296 reticulin and calmodulin were recruited into polyribosomes within 15 min of gravistimulation.

297 d that iLRE-containing mRNAs dissociate from polyribosomes within 20 min after plants are transferred

298 Ultrastructural analysis revealed polyribosomes within growth cones that colocalized with

299 ins and miRNAs biochemically cosediment with polyribosomes, yet another fraction paradoxically accumu

300 cated in the cytosol, and is associated with polyribosomes, yet does not produce protein, indicating