ライフサイエンスコーパス: polysomal

1 These processes correlate with increased polysomal activity of a sequence-selective mRNA endonucl

2 also show that eIF4AIII associates with both polysomal and monosomal RNA in S2 cell extracts, whereas

3 eceptor transcripts were distributed between polysomal and non-polysomal fractions.

4 Conversely, polysomal and post-polysomal sedimentation of the decapp

5 companied by selective 1.5-fold increases in polysomal apoE mRNA levels.

6 echanistically, CGP57380 inhibited efficient polysomal assembly via two processes.

7 s of genes exhibiting increased or decreased polysomal association after Argonaute or RPG knockdowns,

8 of the MLL1 and MLL4 mRNAs increasing their polysomal association and translation, resulting in the

9 o translation analysis and by monitoring the polysomal association of HIF-1alpha mRNA.

10 Second, we show that iron modulates the polysomal association of m-Acon mRNA.

11 Moreover, equol increased the polysomal association of mRNAs for p 120 catenin and eIF

12 pts are functional mRNAs, the expression and polysomal association of natural La1 and La1' RNAs were

13 dent mRNA translation while maintaining mRNA polysomal association.

14 repressed BCL2 mRNA was associated with non-polysomal, but dense fractions on sucrose density gradie

15 Both the abundance and polysomal co-fractionation of CSP1 was enhanced in the c

16 export of COX-2 mRNA into the membrane-bound polysomal compartment at the endoplasmic reticulum.

17 reveal that these proteins are excluded from polysomal complexes in exponentially growing cells, indi

18 the proportion of individual mRNA species in polysomal complexes in leaves of Arabidopsis thaliana un

19 , levels of miR169 pronouncedly decreased in polysomal complexes, concomitant with the increased accu

20 RNAs were associated with the epitope-tagged polysomal complexes, with an average relative level of a

21 Dhh1 and Pat1 are found to co-localize with polysomal complexes.

22 id rRNA pools, and few high-molecular-weight polysomal complexes.

23 We show that the polysomal, cytoplasmic pool of HAC1 mRNA is a substrate

24 lutionarily conserved process leading to the polysomal degradation of thousands of 'non-aberrant' mRN

25 Furthermore, association of the non-polysomal derived proteins to exogenous non-specific mRN

26 Immunoblotting for rpS6 showed modest polysomal disaggregation upon fasting.

27 ith [35S]cysteine/methionine or [14C]NaHCO3, polysomal distribution analyses and ribonuclease protect

28 arisons of protein and mRNA abundance and by polysomal distribution analyses, the uORF represses tran

29 Polysomal distribution analysis of Mrp2 mRNA was consist

30 ere determined by DNA microarray analysis of polysomal distribution.

31 array to monitor the relative abundances and polysomal distributions of mRNAs.

32 have shown that the H19 transcript exists in polysomal form and is likely translated.

33 ha(1)-subunit transcript associated with the polysomal fraction in beta(1)-subunit expressing MSV-MDC

34 , eNOS transcripts were enriched in the free polysomal fraction of nonproliferating cells and enriche

35 s and enriched in the cell matrix-associated polysomal fraction of proliferating cells.

36 studies that demonstrated enrichment of the polysomal fraction of reticulocytes with gamma-globin mR

37 ta-globin pre-mRNA was detected in the light polysomal fraction, and introducing stop codons identifi

38 hown by the increase of CD20 mRNA within the polysomal fraction, indicating a new role of HDAC6 in th

39 dly partitions to the translationally active polysomal fraction.

40 somal fraction to the translationally active polysomal fraction.

41 itions in the translationally inactive, post-polysomal fraction.

42 Polysomal fractionation showed that Mef2c decrease was d

43 Using polysomal fractionation, we demonstrate that the deletio

44 n ribosomal complex stability was studied by polysomal fractionation.

45 -1 alpha (EF1alpha), targeted to the heavier polysomal fractions after a bout of contractions.

46 mRNA shows increased association with these polysomal fractions after PH.

47 mplification of endogenous ATR transcript on polysomal fractions immunoprecipitated with hnRNP A18.

48 7 induced the coshift of Act1 and HuR to the polysomal fractions in a sucrose gradient, HuR deficienc

49 high-throughput deep sequencing analysis of polysomal fractions in cells overexpressing SRSF1.

50 predominantly polysomal fractions toward non-polysomal fractions in cells transfected with the chimer

51 smORF translation by ribosomal profiling of polysomal fractions in Drosophila.

52 oximately 140 kDa in size and is enriched in polysomal fractions of cytoplasmic extracts from HeLa ce

53 Dcp2-like decapping activity was detected in polysomal fractions prepared from mammalian cells.

54 Quantification of alpha-TM mRNA bound in polysomal fractions reveals that both wild-type and hete

55 bution of luciferase mRNA from predominantly polysomal fractions toward non-polysomal fractions in ce

56 at endogenous Nrf2 mRNAs were recruited into polysomal fractions under oxidative stress conditions.

57 xcess mRNAs in rnr1 are often present in non-polysomal fractions, and half-life measurements demonstr

58 the distribution of receptor mRNA toward the polysomal fractions, favoring increased translation.

59 P41a and/or CSP41b, which are not present in polysomal fractions, stabilize ribosome assembly interme

60 ic differences emerged in both monosomal and polysomal fractions.

61 bodies shifted IAP mRNA from nonpolysomal to polysomal fractions.

62 and mRNAs in cytosolic ribonucleoprotein and polysomal fractions.

63 s were distributed between polysomal and non-polysomal fractions.

64 uitylated globin chains are localized to the polysomal fractions.

65 Moreover, polysomal LDH exists in a complex with AUF1 and hsp-70,

66 ARP6 by small interfering RNA also decreased polysomal loading of collagen mRNAs, suggesting that it

67 eIF4E1 protein level by reducing eIF4E1 mRNA polysomal loading without affecting total mRNA level or

68 Polysomal localization of LDH was dependent on RNA bindi

69 fractionation studies were consistent with a polysomal location of hTTP.

70 at could modulate translation initiation and polysomal mRNA assembly.

71 g (RDM), that uses site-specific cleavage of polysomal mRNA followed by separation on a sucrose gradi

72 n on genic regions with fragmented total and polysomal mRNA illuminated numerous aspects of posttrans

73 introduces a new technique for cell-specific polysomal mRNA isolation in kidney injury models that is

74 uld independently, and cooperatively, impair polysomal mRNA loading.

75 A cDNA library, prepared from polysomal mRNA of globular embryos, was screened using a

76 ges in the maternal component of the zygotic polysomal mRNA population during the transition from ooc

77 quantitative comparison of steady-state and polysomal mRNA populations revealed that over half of th

78 usly a cDNA library made from membrane-bound polysomal mRNA prepared from breast and prostate cancer

79 First, using polysomal mRNA profiles, we found that imatinib and CGP5

80 ided a high level of reproducibility between polysomal mRNA samples immunopurified from two independe

81 ted RBP4 in the liver, we isolated total and polysomal mRNA to assess gene-specific translation.

82 Reconstitution experiments in which non-polysomal mRNA-binding proteins are dissociated from the

83 polysomes, or in the number of ribosomes per polysomal mRNA.

84 icant fraction of alphaCP is associated with polysomal mRNA.

85 Quantitative comparison of steady-state and polysomal mRNAs for 15 genes involved in nodulation iden

86 d a methodology for affinity purification of polysomal mRNAs from genetically defined cell population

87 bules from adult testes, and the presence of polysomal mRNAs in sucrose gradient analyses of testes f

88 d metabolites demonstrated that profiling of polysomal mRNAs strongly augments the prediction of cell

89 ally associates with elongating ribosomes on polysomal mRNAs, it specifically auto-phosphorylates on

90 esticular mRNA masking proteins bound to non-polysomal mRNAs.

91 nd in situ translation events validated with polysomal occupancy.

92 n of apoE mRNA in the translationally active polysomal pool.

93 iments with free 40 S ribosomal subunits and polysomal preinitiation complexes, but not with free or

94 Polysomal profiles of myc(-/-) cells revealed decreased

95 In this study, we performed polysomal profiling analysis, which demonstrated HCMV to

96 metabolite quantifications and 13C-tracing, polysomal profiling, and chromatin immunoprecipitation s

97 logous expression, in vitro translation, and polysomal profiling.

98 for functionally related mRNA subgroups and polysomal protein specificity.

99 Analysis of cytoplasmic and polysomal proteins from rat liver after PH showed that C

100 the proportion of individual mRNA species in polysomal (PS) complexes in leaves of non-stressed and m

101 Furthermore, the increased polysomal recruitment of apoE mRNA is largely mediated b

102 ment with these data, Cot/tpl2 increases the polysomal recruitment of the 5 TOP eEF1alpha and eEF2 mR

103 This enzyme, polysomal ribonuclease 1 (PMR1), is a novel member of th

104 n all 3 brain tissues, in both monosomal and polysomal ribosome fractions, we detected an invariant s

105 by ZNF598, ASCC efficiently dissociated all polysomal ribosomes in a stalled queue, monosomes assemb

106 somal subunits but not with 80S monosomes or polysomal ribosomes, indicating that it is not a ribosom

107 somal subunits but not with 80S monosomes or polysomal ribosomes, indicating that it is not a ribosom

108 Rat U34A GeneChips with either total RNA or polysomal RNA at one and six hours following contraction

109 relying on sequencing of total polyA(+) and polysomal RNA from Arabidopsis grown under various condi

110 eloped a one-step procedure for isolation of polysomal RNA from collagen1alpha1-eGFPL10a mice subject

111 e successfully extracted macrophage-specific polysomal RNA from Mac(TRAP) kidneys and conducted RNA s

112 We isolated monosomal and polysomal RNA from seeds at three stages: freshly harves

113 performing microarray analysis of total and polysomal RNA in these cells.

114 wever, comparison of the absolute amounts of polysomal RNA indicates that polysome association is not

115 oligonucleotide microarray hybridization of polysomal RNA of untreated and STI571-treated 32D-BCR/AB

116 Quantitative analysis of sRNAs in total and polysomal RNA samples revealed that mature microRNAs (mi

117 and PABPC1 with both ribosomal subunits and polysomal RNA, an association not previously observed ow

118 Polysomal RNA-based expression profiling with Affymetrix

119 omoter (ProLAT52) to generate epitope-tagged polysomal-RNA complexes that could be affinity purified,

120 Microarray analysis of total and polysomal RNAs nonetheless identified distinct sets of m

121 ity was also found associated with these non-polysomal RNAs.

122 enom exonuclease was used to demonstrate the polysomal RNase generates products with a 3' hydroxyl.

123 This confirms that the polysomal RNase is specific for single-stranded RNA.

124 shed by occlusion of a cleavage site for the polysomal RNase.

125 Targeted analysis of the polysomal RNP complexes revealed that alphaCP is specifi

126 Conversely, polysomal and post-polysomal sedimentation of the decapping proteins was ab

127 undance in the cytoplasmic fraction, and its polysomal sedimentation profile do not change in respons

128 is report, the major proteins binding to non-polysomal testicular mRNAs were isolated and analyzed.

129 ative proteomics, copy number variation, and polysomal transcriptomics.

130 ion by increasing levels of apoE mRNA in the polysomal translating pool.

131 ght conditions by changing the ratio between polysomal versus total messenger RNA.

132 e and chemokine mRNAs in translation-active (polysomal) versus translation-inactive (free ribosomes)