ライフサイエンスコーパス: polysome

1 esis as the nascent proteins emerge from the polysome.

2 ctors may be constitutively localised to the polysome.

3 ns uL4 or uL22 are still capable of entering polysomes.

4 not NCBP2, are components of JUND-containing polysomes.

5 tein 1 (NCBP1)/CBP80 is a component of HIV-1 polysomes.

6 ycolysis decreases TNF mRNA association with polysomes.

7 et of oxidative stress-responsive mRNAs into polysomes.

8 mammalian cells are in turn associated with polysomes.

9 y and the association of the transcript with polysomes.

10 esent in all subpopulations of ER-associated polysomes.

11 se in the amount of grk mRNA associated with polysomes.

12 uces association of the UME6 transcript with polysomes.

13 s with the return of the stabilized mRNAs to polysomes.

14 3 translation and TP53 mRNA association with polysomes.

15 mRNAs into the active translation sites, the polysomes.

16 cies between 30S and 50S subunits versus 70S polysomes.

17 a2(I) mRNA is unrestrictedly loaded onto the polysomes.

18 p1 mutant for membrane-bound, but not total, polysomes.

19 ncreasing the loading of CBP/p300 mRNAs onto polysomes.

20 es with 40S ribosomal subunits and also with polysomes.

21 and it associates with mRNAs assembled into polysomes.

22 existing DHX33 mRNAs to actively translating polysomes.

23 of both PHO1;2 and cis-NATPHO1;2 toward the polysomes.

24 and the loading of CBP/p300 transcripts onto polysomes.

25 nally, IGFBP3 mRNA was found enriched to the polysomes.

26 onosomes and increased their target mRNAs in polysomes.

27 , of mRNAs encoding particular proteins from polysomes.

28 he cell coincident with their depletion from polysomes.

29 the tool to isolate coupled mitochondria and polysomes.

30 ap-dependent manner, were shifted to lighter polysomes.

31 events the escape of immature ribosomes into polysomes.

32 rom ES7, intact 28S rRNA, 80S ribosomes, and polysomes.

33 ges within the chromosomal DNA and among 70S-polysomes.

34 atically linking double-stranded DNA and 70S-polysomes.

35 in the amount of Hsp70 on highly translating polysomes.

36 nscripts were differentially abundant in the polysomes.

37 enriched on ER membrane-bound ribosomes and polysomes.

38 cay (NMD), even though they were detected in polysomes.

39 are necessary to tether JUND-NCBP1/NCBP3 to polysomes.

40 oping multicolor probes, we showed that most polysomes act independently; however, a small fraction (

41 subunits in sucrose gradients and repopulate polysomes after a short starvation-induced translational

42 ADH2 mRNA rapidly dissociated from polysomes after glucose repletion, and sequences bound b

43 This heightened association of miRNA with polysomes also elicits reduced degradation of target mRN

44 Polysome analyses and in vitro reconstitution of initiat

45 Polysome analyses were combined with microarray measurem

46 tive reverse transcriptase PCR (qRT-PCR) and polysome analyses were performed.

47 Polysome analysis indicated that EF-P functions in trans

48 A combined eRIP and polysome analysis of early stage differentiation of hESC

49 Polysome and ribosome profiling revealed that IFIT2 prev

50 ighly hypoxia-induced mRNAs are recruited to polysomes and actively translated, whereas other cellula

51 Cul4B is present on the polysomes and colocalizes with TTP to exosomes and proce

52 e dynamics of messenger RNA association with polysomes and compared the transcriptome with the transl

53 l Ccr4-Not complex in yeast, associates with polysomes and contributes to the negative regulation of

54 levels of actb mRNA associated with synaptic polysomes and diminished levels of synaptic actb protein

55 ranslation by mediating their recruitment on polysomes and enhancing proliferative and self-renewal p

56 ed in increased association of its mRNA with polysomes and led to higher PHO1 protein levels, indepen

57 the SMN mRNA from heavy polysomes to lighter polysomes and monosomes, suggesting that Gemin5 function

58 ssembled ribosomes exist in two populations: polysomes and monosomes.

59 correlated with a global reduction in heavy polysomes and overall repression of translation.

60 Depletion of NAC from polysomes and re-localisation to protein aggregates is o

61 icient in loading uORF-containing mRNAs onto polysomes and stimulates translation in protoplasts, and

62 rent supra-ribosomal building blocks forming polysomes and suggest the presence of unexplored transla

63 In normal conditions, 70S-polysomes and the chromosomal DNA segregate, while 30S a

64 ion influenced recruitment of mRNAs to heavy polysomes and translation of subsets of genes.

65 in annotated 5'-UTRs were overrepresented in polysomes and were as stable as canonical mRNA isoforms.

66 ncrease in monosomes at the expense of large polysomes, and appearance of "halfmer" disomes containin

67 displaces miR-17 from translationally active polysomes, and de-represses multiple miR-17 mRNA targets

68 s are subject to differential recruitment to polysomes, and expose the importance of selective mRNA t

69 own translation, release mRNA molecules from polysomes, and form stress granules (SGs) via a network

70 ssion, promotes cap-dependent translation in polysomes, and reduces the anti-proliferative effect of

71 messenger ribonucleoprotein for loading onto polysomes, and reduction of Cul4B expression shunts the

72 rmore, phosphorylated eIF4E relocates to the polysomes, and this contributes to changes in the transl

73 localized to the cytoplasm, associated with polysomes, and translated to produce E7 oncoprotein.

74 m a stable complex with the 70S ribosome and polysomes, and we demonstrate the proximity in vivo of r

75 iation of the mRNA with actively translating polysomes; and de novo MMP-9 protein synthesis were obta

76 n kinesin-3 and dynein-driven EEs, where the polysomes appeared to translate EE-associated mRNA into

77 We also observe that DYNC1H1 polysomes are actively transported by motors, which may

78 We show that polysomes are less efficient at mediating bypassing than

79 Here we show that polysomes are primarily formed by three distinct classes

80 biquitinated ribosomes are also present in a polysome arrangement, similar to that observed in yeast

81 on microscopy to image ex-vivo-derived human polysomes as a source of actively translating ribosomes.

82 Finally, some DDX6 was associated with polysomes, as previously reported in yeast.

83 ll organization of mRNA and ribosomes within polysomes, as well as the possible role of this organiza

84 crease in mRNA levels as well as a defect in polysome assembly that was independent of mRNA abundance

85 ith eIF4A or eIF4E, and reduces cell growth, polysome assembly, and translation of reporter mRNAs wit

86 n about how proteins emerging from different polysomes associate to form hetero-oligomeric channels.

87 maydis, Higuchi et al. now demonstrate that polysomes associate with early endosomes that undergo ki

88 Collectively, our results indicate that polysomes associate with moving EEs and that "off- and r

89 at the mRNA encoding plexin A remains highly polysome associated during stress and escapes degradatio

90 ticles lacking srRNA 4 and reduced levels of polysome associated ribosomes.

91 NA targeted mRNAs are invariably found to be polysome associated; observations that appear to be at f

92 allel measurements of cytosolic (global) and polysome-associated (translationally enhanced) mRNA leve

93 ARF caused a dramatic reduction in polysome-associated DHX33 mRNAs, while Ras(V12) led to a

94 Genome-wide analysis of cytosolic and polysome-associated messenger RNA (mRNA) revealed not on

95 combinatorial biotin transfer system, where polysome-associated mRNAs are selectively recovered from

96 thms in the protein translation by analyzing polysome-associated mRNAs in the liver of mice fed ad li

97 r, we show enrichment of "model" eRpL22-like polysome-associated testis mRNAs can occur outside the g

98 logy clustering showed that the functions of polysome-associated transcripts differed between dormant

99 The changes in polysome-associated tRNA levels reflect the codon usage

100 anged following viral infection, whereas the polysome-associated tRNA population changes dramatically

101 delineated how a target mRNA passes through polysome association and Ago2 interaction steps on rough

102 and eIF4E2 are elucidated by observing their polysome association and the status of mammalian target

103 easiRNA targeted EVADE genomic RNA, polysome association of GYPSY (ATHILA) subgenomic RNA, a

104 This novel combination results in increased polysome association of mda-7/IL-24 mRNA, activation of

105 Large differences in polysome association of miRNA target RNAs are found betw

106 We next observed that polysome association of nad7 mature mRNA is specifically

107 as promoted by UPF1 depletion, which induced polysome association of PTC-containing mRNAs, increased

108 riptional activity, and had no effect on the polysome association profile of HIF-1alpha.

109 , mRNAs induced upon ERalpha depletion whose polysome association remains unaltered are enriched in c

110 Analyses by RNA gel blot and polysome association suggest that the tRNA deficiencies

111 Reduced polysome association was observed for additional key reg

112 f Hel2 abolished the major 18S crosslink and polysome association, and altered mRNA binding.

113 Transcripts whose levels, but not polysome association, are reduced following ERalpha depl

114 exes from target mRNAs, leading to increased polysome association, translation, and stability of miRN

115 Changes in polysome-bound mRNA (translatome) are correlated closely

116 ling of the rpl4d mutant showed reduction in polysome-bound mRNA compared with wild-type, but no sign

117 RNA-sequencing after isolation of polysome-bound mRNAs allows for cell-type-specific, geno

118 Isolation of pseudopodia-localized polysome-bound RNA reveals transcripts of Lck, Paxillin,

119 Changes in polysome-bound transcripts after miRNA inhibition were d

120 impact on the association of HCMV mRNAs with polysomes but significantly diminished the translation e

121 so determines the specificity of neocortical polysomes by defining their combinatorial composition of

122 nt of elongation, TOP mRNAs are recruited on polysomes causing a relative increase in the synthesis o

123 MD in human cells and found that it triggers polysome collapse characteristic of inhibited translatio

124 on and are a result of its direct binding to polysomes, complex formation with cellular RNAs (which i

125 lyzing mRNAs that dynamically associate with polysome complexes as neocortical development progresses

126 a-up-regulated gene transcripts increased in polysome complexes during the stress, but the number of

127 Ribosomal protein specificity within polysome complexes is regulated by the arrival of in-gro

128 s bind target mRNAs in high molecular weight polysome complexes, while inhibited miRNAs are stericall

129 as a shift of TDP-43 and FUS mRNAs away from polysomes, consistent with translational silencing.

130 Polysomes contain ~1 ribosome every 200 to 900 nucleotid

131 The actively translating ribosomes (polysomes) contain amounts of proteins consistent with t

132 show that active mRNA translation complexes (polysomes) contain ribosomal protein subsets that underg

133 location of longer 3' UTR mRNAs from RNPs to polysomes correlated with the production of new miRNAs t

134 Association of mRNA with the polysomes correlates with active translation.

135 a reduction in 40S subunits and translating polysomes, correlating with reduced overall cellular pro

136 miRNA profiling of monosomes and polysomes demonstrated that miRNAs cosediment with ribos

137 hinery concomitantly inhibits stress-induced polysome disassembly and SG assembly.

138 Following translation inhibition, polysome disassembly releases 48S preinitiation complexe

139 K85R) mutant do not prevent arsenite-induced polysome disassembly, but fails to support the SG assemb

140 ation of SG proteins to VFs was dependent on polysome dissociation and occurred via association of th

141 y performing mRNA half-life measurements and polysome distribution analysis, we found that AUF1 assoc

142 es, including involucrin, are bound to heavy polysomes during differentiation, despite decreased gene

143 3'-UTRs were preferentially associated with polysomes during oxidative stress.

144 n and identified conditions that distinguish polysomes engaged in the translation of distinct cohorts

145 ly, the Sec61 complex was highly enriched in polysomes engaged in the translation of endomembrane org

146 as a direct HIF-1alpha target gene and that polysome enrichment of IGFBP3 mRNA may permit continuous

147 Hfq from polysomes extracted from wild-type cells binds RyhB in v

148 for translocation of viral RNA genome to the polysomes for efficient translation and replication.

149 d to accommodate a maximum of membrane-bound polysomes for secretory protein synthesis.

150 miRNA binding sites and are thus targeted to polysomes for translation followed by degradation.

151 transport and disrupting their loading onto polysomes for translation.

152 acetylation does not significantly alter the polysome formation rate on Nmnat mRNAs.

153 0S ribosomal subunits that were blocked from polysome formation.

154 ing with labeled ribosomes to determine when polysomes formed.

155 Importantly, their localisation to the polysome fraction is independent of the RNA, suggesting

156 nd that decapping factors co-sediment in the polysome fraction of a sucrose gradient and do not alter

157 abundance of these target transcripts in the polysome fraction of TcUBP1-induced samples.

158 -untranslated region (UTR), as determined by polysome fractionation and 5'UTR-reporter assays.

159 cover by integrating large scale proteomics, polysome fractionation and a focused RNAi approach that

160 Polysome fractionation experiments confirmed eIF4E could

161 Polysome fractionation experiments indicate that dFMRP O

162 as indicated by luciferase reporter assays, polysome fractionation studies, and Western blot analysi

163 L-seq (TATL-seq), which combines TL-seq with polysome fractionation, enabled annotation of TLs, and s

164 Polysome fractionations coupled with quantitative PCR ex

165 creased viral messenger RNA association with polysome fractions and enhanced tolerance to begomovirus

166 omic analyses of HeLa cytosolic and ER-bound polysome fractions identified translocon components as s

167 Polysome gradient analyses of mutant strains revealed un

168 r by evaluating the position of an mRNA in a polysome gradient.

169 tates, we measured mRNA profiles on ribosome/polysome gradients by RNA sequencing (RNA-seq).

170 mRNAs can be packaged in reversibly stalled polysome granules before their transport to distant syna

171 Using RNA-seq analysis of mouse neocortical polysomes, here we report translationally repressed and

172 e reconstitute the disassembly of a collided polysome in a mammalian cell-free system.

173 ment by counting the number of ribosomes per polysome in early and late embryos.

174 zation of RP transcripts led to retention of polysomes in a hog1Delta mutant, whereas stabilization o

175 te only in skeletal muscle, without reducing polysomes in any tissue.

176 and dynamic recruitment of messenger RNAs to polysomes in both dormant and nondormant seeds.

177 Furthermore, Htt co-sedimentation with polysomes in cytoplasmic mouse brain extracts is depende

178 nt local translation and active transport of polysomes in dendrites of primary neurons.

179 s, capture distinct mobilities of individual polysomes in different subcellular compartments, and det

180 munopurification of transcripts engaged with polysomes in pollen tubes within self-fertilized florets

181 A decay, is critical for assembly of stalled polysomes in rat hippocampal neurons derived from embryo

182 upregulated at the level of association with polysomes in response to rhizobia.

183 -'UTR-shortened transcripts efficiently form polysomes in the mTOR-activated cells, leading to increa

184 F3-containing terminating ribosomes in heavy polysomes in vivo indicating slower termination rates, t

185 The apparent formation of polysomes in wheat germ extracts suggests that subsequen

186 RNA sequencing of polysomes indicated substantial overlap in sets of genes

187 ing RNAs (lncRNAs) are often associated with polysomes, indicating coding potential.

188 nosines ((Cap)2G or (Cap)3G) are enriched on polysomes, indicating that RNAs synthesized from differe

189 dentified translocon components as selective polysome-interacting proteins.

190 mRNAs associated with translationally active polysomes, irrespective of unchanged total HSP90 levels.

191 elta cells, but their rapid association with polysomes is delayed.

192 strated that the transport of HCV RNA on the polysomes is Stau1-dependent, being mainly localized in

193 The association of proteasomes with polysomes is weakened in srp1-49.

194 ly detected in multiple tissues, and also in polysome isolated from leaf, confirming active transcrip

195 these RNA isoforms were underrepresented in polysomes isolated from control and hypoxic plants.

196 In Drosophila, reduced IIS decreased polysome levels in fat body and gut, but reduced the rat

197 the association of eIF2 subunits, diminished polysome levels, and increased GCN4 expression indicatin

198 HuR-dependent proteins, the association with polysomes likewise depends on the eukaryotic initiation

199 directly binds Mre11 transcripts to mediate polysome loading and translation.

200 Modeling revealed that a decrease in polysome loading at night is required to balance protein

201 ases, rising metabolite levels and increased polysome loading drove an increase in fluxes.

202 tantly, the fold induction of TNF-alpha mRNA polysome loading in response to LPS stimulation is reduc

203 Leaf elongation and polysome loading in the growth zones also remain high at

204 demonstrate the essential role of selective polysome loading in this biological process.

205 inst even sudden changes in temperature, and polysome loading increases when the night temperature is

206 tion-polymerase chain reaction revealed that polysome loading remained high for much of the night in

207 In Col-0, polysome loading was 60% to 70% in the light, 40% to 45%

208 Polysome loading was investigated during the diurnal cyc

209 nses of the metabolome and transcriptome and polysome loading, as a qualitative proxy for protein syn

210 h is mechanistically traced to a decrease in polysome loading.

211 t correlated with overall and with cytosolic polysome loading.

212 med target mRNA localization to the ER-bound polysomes manifested as the earliest event, which is fol

213 We propose that polysomes may act as antennae that enhance the rates of

214 creasing the amount of SNCA mRNA loaded into polysomes, mechanistically providing a cytoprotective ef

215 m(6)A in Snail CDS, but not 3'UTR, triggers polysome-mediated translation of Snail mRNA in cancer ce

216 ell line to fit thousands of images of human polysomes obtained by atomic force microscopy, from whic

217 Both signaling pathways enhanced polysome occupancy and eukaryotic initiation factor 4E (

218 The effect of Jak-Stat-Pim signaling on polysome occupancy and expression of GW182 protein was g

219 cells binds RyhB in vitro, whereas Hfq from polysomes of a relA mutant strain shows no binding.

220 s with short half-lives were enriched in the polysomes of upf1 mutants, indicating that UPF1/NMD supp

221 ail lengths were similar for target mRNAs on polysomes or in non-translating mRNPs, and the presence

222 of Snail1 mRNA into the actively translated polysome pool accompanied by accumulation of the EMT tra

223 regulated TCF7L2 mRNA association with heavy polysomes, probably through the TCF7L2 5'-untranslated r

224 Polysome profile analyses reveal the accumulation of hal

225 We fractionated a polysome profile and reconstructed transcript isoforms f

226 k6 plants accumulate 80S ribosomes while the polysome profile remains unchanged, consistent with a ph

227 Furthermore, we also used a polysome profile, supporting that YM155 markedly suppres

228 We investigated seed polysome profiles and the mRNAs and protein complexes th

229 Polysome profiles confirmed the decreased translational

230 Analyses of polysome profiles revealed that key differentiation gene

231 sibly blocks ribosomal elongation, preserves polysome profiles, and may be a broadly useful tool for

232 phase cell count, ploidy levels and ribosome polysome profiles.

233 in proplatelet-producing MKs in 2D DIGE and polysome profiling analyses, respectively.

234 of Satb1 transcript variants as confirmed by polysome profiling and in vitro translation assay.

235 analysis of CD8 T-cell activation, combining polysome profiling and microarray analysis.

236 By combining polysome profiling and next-generation sequencing, ILF3

237 ss of PUF3 on gene expression using mRNA and polysome profiling and quantitative proteomics.

238 -)), and two independent translation assays, polysome profiling and radiolabeled amino acid incorpora

239 Furthermore, using polysome profiling and ribosome profiling, we failed to

240 Combining polysome profiling and RNA sequencing, we identified the

241 We used polysome profiling by RNA-Seq to quantify translational

242 f U2AF1 is altered by the S34F mutation, and polysome profiling indicates that the mutation affects t

243 Polysome profiling indicates that UBAP2L promotes transl

244 Here we combine polysome profiling of a library of 280,000 randomized 5'

245 Polysome profiling of the rpl4d mutant showed reduction

246 Mechanistically, polysome profiling revealed reduced translation of genes

247 Polysome profiling revealed that translation of GluA1 an

248 Here, we show by polysome profiling that inhibin betaA is translationally

249 blastoma to ionizing radiation (IR), we used polysome profiling to define the IR-induced translatomes

250 2D difference gel electrophoresis (DIGE) and polysome profiling, respectively.

251 We utilized polysome profiling, RNA sequencing, and whole-proteomic

252 Using Structure-seq2 and polysome profiling, we assess global mRNA structure and

253 Using transcriptome-wide RNA-Seq and polysome profiling-Seq in halofuginone-treated fibroblas

254 ight stress in Arabidopsis thaliana by using polysomes profiling.

255 l oligopyrimidine motif) and TOP-like mRNAs, polysome-profiling indicated that MTOR also modulates tr

256 this regulation, we developed a genome-wide polysome-profiling strategy using stage-matched WT and e

257 In untreated conditions, Puf3p migrates with polysomes rather than ribosome-free fractions, but this

258 dependent 5'TOP mRNA translation repression, polysome release, and accumulation in stress granules.

259 tion of factor XII (FXII) messenger RNA with polysomes, resulting in increased translation.

260 isoform mRNAs lost from the eif3ha morphant polysomes, revealing a mechanism by which lens developme

261 action, which we term Transcript Isoforms in Polysomes sequencing (TrIP-seq).

262 Overall polysome sequestration contributes to an increase of cel

263 Here we report a novel method--miRNA Polysome Shift Assay (miPSA)--for direct measurement of

264 ffects of eIF5A depletion are a reduction in polysome size concomitant with eIF5A depletion.

265 -intact tissue culture cells, we performed a polysome solubilization screen and identified conditions

266 The potential role of the polysome structure during protein translation and the re

267 lored translational controls embedded in the polysome structure.

268 mRNA stability and polysome studies suggest IGF2BP1 mediates posttranscript

269 NS5A associated with eIF4F complex and polysomes, suggesting its active involvement in host tra

270 th retained U12-type introns can be found in polysomes, suggesting that splicing efficiency can alter

271 umulation of 70S monosomes at the expense of polysomes, suggesting that the growth defect stems from

272 decreasing the proportion of mef2ca mRNA in polysomes, the levels of Mef2c and slow myosin heavy cha

273 nge in the levels of Ribosomal protein L7 in polysomes, thereby regulating neocortical translation ma

274 After stimulation, CPEB3 is recruited into polysomes, thus promoting the translation of its target

275 esponded with a shift in cyclin B1 mRNA from polysome to nonpolysome fractions.

276 Gemin5 redistributes the SMN mRNA from heavy polysomes to lighter polysomes and monosomes, suggesting

277 further examined their potency at converting polysomes to monosomes across other commonly used model

278 s manifested by a global shift in mRNAs from polysomes to monosomes and the downregulation of genes i

279 osomal integrity while thoroughly converting polysomes to monosomes in all examined species.

280 type cells and the shift in translation from polysomes to monosomes is attenuated, suggesting puf3Del

281 g TNFalpha mRNA from polyribosome-associated polysomes to monosomes.

282 bosomal particles and complete conversion of polysomes to monosomes.

283 forms of recapping targets redistribute from polysomes to non-translating mRNPs, and recapping is all

284 he redistribution of target transcripts from polysomes to non-translating mRNPs, where they accumulat

285 futsch mRNA shift from actively translating polysomes to nontranslating ribonuclear protein particle

286 ng RNAs) with 50% being enriched on specific polysome types.

287 (Dc,60S = 0.311 microm(2)/s), whereas entire polysomes underwent long-range motility along microtubul

288 Upon release from polysomes, unfolded mRNAs outcompete G3BP auto-inhibitor

289 rmine the partitioning of ribosomes into 70S-polysomes versus free 30S and 50S subunits.

290 percentage of the HAC1 mRNA associating with polysomes versus free ribosomal subunits.

291 ned that CSP1 associates with polyribosomes (polysomes) via an RNA-mediated interaction.

292 ation, the association of mRNAs and sRNAs to polysomes was characterized in roots of the model legume

293 immediate-early, early, and late mRNAs with polysomes was not affected by eIF4F disruption.

294 wnregulated and exclusively localized in the polysomes when Stau1 is overexpressed.

295 -independent manner, were shifted to heavier polysomes whereas mRNAs encoding GAPDH, actin, L32, and

296 ment of early endosomes randomly distributes polysomes, which may ensure the even distribution of the

297 rangement, similar to that observed in yeast polysomes, which we determined using cryoelectron tomogr

298 s C), but did not influence global levels of polysomes, which were minimally perturbed by above freez

299 Cbc1 associates with polysomes, while the deletion of the CBC1 gene causes hy

300 Loss of polysomes with increased 80S monosomes in the polyamine-