ライフサイエンスコーパス: population doubling

1 SC-derived epicardial cells (for at least 25 population doublings).

2 rm culture (50 passages or approximately 260 population doublings).

3 ines when treated with IdUrd or BrdUrd for 1 population doubling.

4 ost 50-60 nt of telomeric CCCTAA repeats per population doubling.

5 by 40% to 45% in just 2 days, one usual cell population doubling.

6 hological transformation after more than 100 population doublings.

7 nently contain short telomeres after only 40 population doublings.

8 ersisted for up to 7 days, equivalent to six population doublings.

9 ted cells can be maintained for at least 130 population doublings.

10 tion doublings remaining) to greater than 90 population doublings.

11 erase (hTERT) have been followed for 250-400 population doublings.

12 ntained at their new lengths for at least 20 population doublings.

13 ferate, and two have completed more than 110 population doublings.

14 e become senescent after a limited number of population doublings.

15 nt or apoptotic and ceased growth within 3-4 population doublings.

16 maintained HPV plasmid persistence beyond 60 population doublings.

17 irreversible cell cycle arrest after several population doublings.

18 aining linear growth curves over at least 30 population doublings.

19 licative senescence after a finite number of population doublings.

20 logy and growth response after more than 100 population doublings.

21 itor cells (MAPCs), can be expanded for >120 population doublings.

22 a telomere reduction rate of 500 base pairs/population doubling, a rate that is much faster than exp

23 cease proliferation after a finite number of population doublings, a phenomenon termed replicative se

24 ic muscles achieved at least five additional population doublings above the maximum that was attained

25 Exposure to Ang II over several population doublings accelerated the rate of telomere at

26 Cells in OpM1 reached 1.74 population doublings after 30 min, more than twofold hig

27 d foci in this system is delayed for several population doublings after irradiation and appears to in

28 in mitotic control were evident within 5-10 population doublings after retroviral infection, indicat

29 d from 3 to 5 times, with an average of 5.99 population doublings after subculturing, and a lifespan

30 BMR cells proliferated actively for 7-20 population doublings, after which the cells began secret

31 a consequence of genetic abnormalities many population doublings ago, not from the antiestrogen-sens

32 that WJ MSC from obese donors showed slower population doubling and a stronger immunosuppressive act

33 We report a delayed population doubling and accelerated senescence in Aptx-/

34 flavopiridol results in a 3-fold decrease in population doubling and prevents recovery from treatment

35 ten their telomeres at an increased rate per population doubling and the premature senescence this lo

36 thout loss of multipotency for more than 140 population doublings and exhibit the capacity for differ

37 TRF1 could be maintained over more than 300 population doublings and resulted in stable, short telom

38 nger proliferative lifespans, underwent more population doublings, and experienced senescence later,

39 and cell cycle kinetics at greater than 240 population doublings, and loss of p16 after passage 10.

40 expand for an extensive but finite number of population doublings, and maintain a diploid karyotype b

41 roliferative cells emerge to undergo further population doublings (approximately 20-70), before enter

42 cose (average of 10-fold) was stable over 66 population doublings (approximately 7.5 months of tissue

43 sgenic mice and expanded in vitro for >40-80 population doublings, are capable of multilineage hemato

44 term replicative capacity (approximately 80 population doublings) arose from these growth arrested c

45 ferative potential of approximately 10 to 40 population doublings before encountering a stress-associ

46 n be grown in culture for a finite number of population doublings before they cease proliferation and

47 an undifferentiated state for more than 100 population doublings, can thus differentiate into cells

48 Growth kinetics and population doubling capacity were assessed by passaging

49 A 0.8-kb cDNA clone, isolate EPC-A2 (early population doubling cDNA-A2), encodes the 3' end of the

50 After 30-40 population doublings cells became growth-arrested in G0/

51 a uniform, fixed number of approximately 50 population doublings, commonly termed the Hayflick limit

52 enescent cells, reflecting a lower number of population doublings completed by the former.

53 A significant decrease in EC cumulative population doublings (CPDs) was associated with the rare

54 By 250 population doublings, dramatic two- and fourfold length

55 tend fibroblast lifespan by approximately 10 population doublings, ending in a viable senescence-like

56 significantly increased the total number of population doublings exhibited by OPCs before mitotic se

57 , we conducted a long-term (> 3 months; > 40 population doublings) experiment in which normal immorta

58 C) initiated as primary cultures to 50 to 60 population doublings followed by p16-positive, senescenc

59 Within one population doubling following Rev3L deletion, DNA double

60 ethylation in the MGMT CpG island of cells 4 population doublings following replating after confluenc

61 noted in logarithmically growing cells by 10 population doublings following replating.

62 failed to explain why it took more than "60 population doublings" from the introduction of the first

63 ells and the shortening of telomeres at each population doubling have suggested that telomere length

64 ave been maintained in culture for up to 100 population doublings, have a high self-renewal capabilit

65 underwent replicative senescence after 65-70 population doublings; however, they continued expression

66 Thus, despite dividing 40 population doublings, hTERT strains derived from single

67 occurs at a rate of about 10(-2)-10(-3) per population doubling in recent isolates from nature, but

68 r than the already rapid growth rate of 0.74 population doublings in 30 min in M1.

69 rapid outgrowth and expansion of SOC to >40 population doublings in a 4-month period.

70 henotypic stability after expansion for >150 population doublings in a serum-free, defined medium and

71 HSV-tk mutant frequencies measured after 10 population doublings in cells derived from a clinically

72 merase hTERT gene, and growing them for many population doublings in culture, Serakinci et al observe

73 the demethylated fibroblasts for up to five population doublings in culture.

74 et of a senescence phenotype following 15-25 population doublings in culture.

75 d was then stably maintained for at least 60 population doublings in culture.

76 rrant proliferative signals or by cumulative population doublings in culture.

77 xtended by nuclear transfer, as indicated by population doublings in fibroblast lines derived from a

78 e context (PMD solo-WCGWs) tracks cumulative population doublings in primary cell culture.

79 ulse of hormone is stably maintained for >50 population doublings in the absence of hormone in clonal

80 The T(CM) were capable of 20-40 mean population doublings in vitro, and the expanded cells pr

81 populations are strongly modulated over >15 population doublings in vitro.

82 vessel walls can be passaged for at least 40 population doublings in vitro.

83 c cells can undergo only a limited number of population doublings in vitro.

84 st termed M0 (mortality stage 0) after 10-15 population doublings in vitro.

85 The maximum number of population doublings increased from 5 doublings in M1 to

86 expression of mutant p53 proteins for 16-17 population doublings increased the frequency of appearan

87 ogy and a significant reduction in mobility, population doubling, increased levels of reactive oxygen

88 /FN123 cells had accelerated growth, greater population doublings, increased telomerase activity, and

89 elo-seq resolves telomere shortening in five population doubling increments and reveals intrasample,

90 lenium supplementation extends the number of population doublings, its deficiency impairs the prolife

91 with crisis occurring approximately 10 to 25 population doublings later than in Upf+ cells.

92 early passages [approximately 30 cumulative population doubling level (cpdl)] showed a low level of

93 showed decreasing TRF lengths from 11 kbp at population doubling level (PDL) 15 to 9.5 kbp at PDL 73.

94 galactosidase-labeled cells as a function of population doubling level (PDL).

95 EPC-1/PEDF (early population doubling level cDNA-1/retinal pigmented epith

96 Early population doubling level complementary DNA-1 (EPC-1, an

97 Analysis of HDFs at increasing population doubling levels shows a gradual increase in s

98 After reaching the population doubling limit of their parent cell strains,

99 nt (LTC) cultures completed 15-25 fewer mean population doublings (MPDs) than the controls prior to s

100 rol efflux was inversely correlated with the population doubling number in cell culture and was inhib

101 Population doubling occurred significantly faster in cul

102 at least 20 passages with a mean cumulative population doubling of 50.3 +/- 4.5.

103 lular levels of p300 and CBP with increasing population doublings of human normal melanocytes.

104 ressing telomerase showed an increase of 5-9 population doublings over 234 days of culture in monolay

105 es between that of normal fibroblasts [99 bp/population doubling (PD)] and four times that of normal

106 n of both permitted cells to divide 25 to 43 population doublings (PD) beyond their normal limit.

107 thyroglobulin expression, capable of 10 - 15 population doublings (PD) compared to less than three in

108 EC could be cultured for at least 15-20 more population doublings (PD) than control cells.

109 These cells have continued to grow over 300 population doublings (PD) with no signs of senescence, w

110 ns only support cell proliferation for 30-40 population doublings (PD), a point at which telomeres re

111 sitive LacZ21 cells remained below 2% for 25 population doublings (pd), first became significantly in

112 res are capable of robust and long-term [>70 population doublings (PD)] proliferation in culture, hav

113 plicative ability and senesce after 50 to 60 population doublings (PD)s.

114 n IMR90 fibroblasts in tissue culture by >20 population doubling (PDLs).

115 These effects were observed by 25 population doublings (PDs) following the establishment o

116 -derived stem cells (MDSCs) expanded for 300 population doublings (PDs) showed no indication of repli

117 After a limited number of population doublings (PDs), cultures of normal mammalian

118 red an additional 28 +/- 1.5 and 3.4 +/- 0.4 population doublings (PDs), respectively.

119 dium on plastic senesced at approximately 13 population doublings (PDs).

120 Long-term cultures (> 50 population doublings [PDs]) were evaluated for transform

121 t 4 kb (from 12.8 to 8.8 kb) over 1 year (89 population doublings [PDs]).

122 Most of the control clones underwent about 2 population doublings per day (PD/D).

123 e growth rate is reduced from seven to eight population doublings per week to one to two doublings pe

124 cing of these cells after defined numbers of population doublings permitted analysis of nascent mutat

125 ol colonies failed to expand beyond 32 to 36 population doublings postexplantation.

126 SCs expressed lower colony-forming capacity, population-doubling rate, cell proliferation, multipoten

127 in ddG caused no observable effects on cell population doubling rates or morphology.

128 end telomeres shortened by around 100 nt per population doubling, reflecting the generation of 3' ove

129 e life-span of senescent cells (zero to four population doublings remaining) to greater than 90 popul

130 in cord blood that can achieve at least 100 population doublings, replate into at least secondary an

131 Even after 40 population doublings, replication, abundance of the rapi

132 NEK4 suppression extended the number of population doublings required to reach replicative senes

133 Lines maintained for over 250 population doublings retained long telomeres and a norma

134 c fibroblasts and/or tissues display reduced population doubling, significantly dampened DNA damage r

135 port here that at twice the normal number of population doublings, telomerase-expressing human skin f

136 s (rBMSC) underwent significantly more total population doublings than human BMSCs (hBMSC) and rhesus

137 increased annual reproductive effort across populations, doubling the proportion of plants in flower

138 without obvious senescence for more than 80 population doublings, they may be an important source of

139 ight SDH activity correlated positively with population doubling time (R(2) = 0.91 for PAC, 0.76 for

140 beyond that of control was achieved without population doubling time acceleration.

141 showed an approximately 1.5-fold increase in population doubling time and a 2-fold reduction in mitot

142 or GFP-DeltaHP1(Hsbeta) showed a decrease in population doubling time and decreased sensitivity to IR

143 An increase in the cell population doubling time and higher sensitivity to cell

144 Population doubling time averaged 27 hours in mCSCs and

145 The population doubling time is approximately 4 days.

146 SHI cells displayed a population doubling time of 29 h compared with 19 h for

147 ca-1(+), shared similar morphology and had a population doubling time of approximately 2 days.

148 -(4-sulfophenyl)-2H-tetrazolium] assay, cell population doubling time), apoptosis, and colony formati

149 We estimated cell energy consumption by population doubling time, and cell survival and growth b

150 ing IGFBP-rP1 had a dose-related increase in population doubling time, decreased colony formation in

151 decreased plating efficiency, elongated cell population doubling time, lower clonogenic fraction in s

152 rested by serum deprivation after which cell population doubling time, proliferation fraction, and ce

153 verexpression influences telomere stability, population doubling time, radioresistance, and tumorigen

154 mmortalized cells in terms of proliferation, population doubling time, soft agar growth, transwell mi

155 ctors such as p53 mutation status and native population doubling time.

156 e with FGF-2 alone and exhibited a decreased population doubling time.

157 ferating cells with no effect on the overall population doubling time.

158 een mutation burden and lifespan, as well as population doubling time.

159 eased plating efficiency; (b) elongated cell population doubling time; (c) lower clonogenic fraction

160 xhibit cell cycle defects, including reduced population-doubling time and a delay in cell cycle reent

161 eases anchorage-independent growth, shortens population-doubling time and reduces contact inhibition.

162 The population-doubling time of exponentially growing cells

163 The telomere-telomerase axis, population-doubling time, and insulin-like growth factor

164 To document the growth kinetics of CSCs, population-doubling time, telomere length, telomerase ac

165 tants for suppression"), displayed a reduced population-doubling time, with 9 of 18 showing focus for

166 hondria incorporated thymidine-3H within one population-doubling time.

167 mber of oligodendrocytes in these clones and population doubling-time.

168 The population doubling times (DT) of sham-irradiated cells

169 extrachromosomal HR HPV genomes had shorter population doubling times and formed dysplastic stratifi

170 The double-expressor cells had similar population doubling times and were as infective as wild-

171 approximately once per day, while commensal population doubling times are likely on the order of min

172 Exogenous and endogenous apoE increased population doubling times by 30-50% over a period of 14

173 d anchorage-independent growth and decreased population doubling times in estrogen-depleted or 4-hydr

174 Cell population doubling times were determined and correlated

175 These cells also exhibited longer population doubling times which did not arise through re

176 00 microM TMPyP4 over 15 days in culture (10 population doubling times).

177 nd on the specific cell type used and on its population-doubling times so that the required numbers o

178 airable ROS-induced DSB and greatly improved population-doubling times.

179 determined by calculating the ratio of mean population doublings to the number of days required for

180 ., the inability, after a critical number of population doublings, to replicate) on microvascular rem

181 so decreased cell survival and the number of population doublings under suboptimal culture conditions

182 ate, the slope of the line on a plot of cell population doublings versus time, as an alternative, tim

183 However, the rate of base pair loss per population doubling was significantly lower during the f

184 erage chromosomal telomere length at several population doublings was estimated by Southern blot anal

185 be stably propagated for >12 passages or 30 population doublings while retaining their dual commitme

186 ce they have been passaged for more than 100 population doublings without any diminution in growth po

187 dle-shaped morphology; self-renewal for >200 population doublings without evidence for senescence; no

188 ous hmTert alleles proliferated for over 400 population doublings without exhibiting chromosomal abno

189 ll line is capable of replicating beyond 100 population doublings without exhibiting signs of senesce