ライフサイエンスコーパス: pre-rRNA

1 osomal subunit assembly, accumulation of 12S pre-rRNA, and impaired erythropoiesis.

2 a pre-rRNA processing step that produces 12S pre-rRNA, a precursor to the 5.8S rRNA. However, a heter

3 trates delayed kinetics of 35S, 27S, and 20S pre-rRNA processing with turnover of these intermediates

4 mpaired growth and defective cytoplasmic 20S pre-rRNA processing at 16 degrees C and 20 degrees C.

5 utant strain, showing an accumulation of 20S pre-rRNA and a 40S export defect.

6 own to be required for the conversion of 20S pre-rRNA to 18S rRNA.

7 a role for rpS14 in 3' end processing of 20S pre-rRNA.

8 -resolution kinetic labeling showed that 20S pre-rRNA predominately undergoes methylation as nascent

9 opose that Fap7 mediates cleavage of the 20S pre-rRNA at site D by directly interacting with Rps14 an

10 is strictly required for cleavage of the 20S pre-rRNA at site D in the cytoplasm.

11 PIN-domain endonuclease Nob1 cleaves the 20S pre-rRNA at site D, to form the mature 18S rRNAs.

12 sequence and structural features of the 20S pre-rRNA near the cleavage site of the nuclease, Nob1.

13 of Fap7 causes accumulation of only the 20S pre-rRNA, which could be detected not only in 43S prerib

14 9 is critical for timely cleavage of the 20S pre-rRNA.

15 NA hairpins that incorporate the 16S and 23S pre-rRNA stem sequences are efficiently cleaved by Tm-RN

16 nR64 fails to methylate residue C2337 in 27S pre-rRNA, suggesting a role in snoRNA function.

17 Polyadenylated forms of the 27S pre-rRNA and the 25S rRNA were detected, suggesting the

18 ermore, in the absence of Has1, aberrant 27S pre-rRNAs are targeted for irreversible turnover.

19 is defective in the formation of 20S and 27S pre-rRNAs and in the accumulation of 18S and 25S mature

20 ty of functional preribosomes containing 27S pre-rRNAs.

21 Prp43p-Q423N mutant immunoprecipitates 27SA2 pre-rRNA threefold more efficiently than the wild type,

22 re the ITS1 spacer can be removed from 27SA3 pre-rRNA.

23 ng triggers exonucleolytic trimming of 27SA3 pre-rRNA to generate the 5' end of 5.8S rRNA and drives

24 ent recruitment of factors required for 27SB pre-rRNA processing, namely, Nsa2 and Nog2, which associ

25 role in removal of the ITS2 spacer from 27SB pre-rRNA.

26 molecules with pre-rRNPs, processing of 27SB pre-rRNA is blocked.

27 e required for downstream processing of 27SB pre-rRNA.

28 rgely blocked, as was maturation of the 27SB pre-rRNA to the 5.8S and 25S rRNAs.

29 pre-60S ribosomal particles containing 27SB pre-rRNAs.

30 tion of NOP12 significantly inhibits 27SA(3) pre-rRNA processing, even though the A(3) factors are pr

31 which is necessary for conversion of 27SA(3) pre-rRNA to 27SB(S) pre-rRNA.

32 somal subunit assembly-processing of 27SA(3) pre-rRNA.

33 The longer 5.8S+30 pre-rRNA (a form of 5.8S rRNA 3' extended by approximate

34 Nog1 function inhibits the conversion of 32S pre-rRNA-containing complexes to a smaller form, resulti

35 onditional prp43 mutant alleles confer a 35S pre-rRNA processing defect, with subsequent depletion of

36 cold-sensitive prp43 mutant accumulates 35S pre-rRNA and depletes 20S, 27S, and 7S pre-rRNAs, precur

37 t the A0, A1, and A2 processing sites in 35S pre-rRNA, delayed processing of 20S rRNA to mature 18S r

38 negative mutants delay processing of the 35S pre-rRNA and cause accumulation of pre-rRNA species that

39 of contranscriptional processing of the 35S pre-rRNA in budding yeast is unclear.

40 e 2 cleavage on ITS1 of 47S/45S, 41S and 36S pre-rRNAs in human cells.

41 ated the site 2 cleavage of 47S, 45S and 41S pre-rRNAs.

42 d with wild-type ES cells, the levels of 45S pre-rRNA are reduced in both Chd7(+/-) and Chd7(-/-) mou

43 expressing CHD7 show increased levels of 45S pre-rRNA compared with control cells.

44 promoter and a concomitant reduction of 45S pre-rRNA levels.

45 5'-external transcribed spacer (ETS) of 45S pre-rRNA, as MTR4 does.

46 CX treatment reduced 45S pre-rRNA expression (-64 +/- 5% vs. IGF-1; P < 0.001) an

47 umulation of the 5' extended from of 45S/47S pre-rRNA and 5'-01, A0-1 and E-2 fragments of pre-rRNA t

48 ls results in a significant reduction in 47S pre-rRNA levels, whereas synthesis of the first 40 nt of

49 ge ribosomal subunit (50S) rRNAs, 23S and 5S pre-rRNAs, are catalyzed by the double-strand specific r

50 B treatment rapidly blocked processing of 6S pre-rRNA to 5.8S rRNA, leading to TRAMP-dependent pre-rR

51 nuclear pre-60S particles containing the 6S pre-rRNA bind Nmd3 and Crm1 and are exported to the cyto

52 The 6S pre-rRNA was coprecipitated with the 60S export adapter

53 the RNase responsible for processing the 7S pre-rRNA to the mature 5.8S rRNA.

54 ucture assembled around the 3' end of the 7S pre-rRNA.

55 s 35S pre-rRNA and depletes 20S, 27S, and 7S pre-rRNAs, precursors to the small- and large-subunit rR

56 aining 27SA2, 27SA3, 27SB, and 25.5S plus 7S pre-rRNAs plus ribosome assembly factors and ribosomal p

57 ergence of a novel factor that facilitates a pre-rRNA processing event specific for higher eukaryotes

58 BCCIP is vital for a pre-rRNA processing step that produces 12S pre-rRNA, a p

59 eiotropic developmental defects and aberrant pre-rRNA processing.

60 Strikingly, degradation of many aberrant pre-rRNA species, attributed mainly to 3' exonucleases i

61 the dominant negative mutations also altered pre-rRNA processing.

62 cruitment of pol I transcription factors and pre-rRNA processing factors to elongating pre-rRNA on an

63 nd show that the mutation confers growth and pre-rRNA processing defects.

64 nd analyzed their effects on cell growth and pre-rRNA processing.

65 ce factor, DNA-dependent RNA polymerase, and pre-rRNA processing protein revealed the isolate to be a

66 g telophase when rDNA gene transcription and pre-rRNA methylation are known to commence.

67 results indicate that rRNA transcription and pre-rRNA processing are coordinated via specific compone

68 coordination between rDNA transcription and pre-rRNA processing in mammalian cells.

69 coordinating ribosomal DNA transcription and pre-rRNA processing to allow for the efficient synthesis

70 ic neurons results in defective pre-tRNA and pre-rRNA processing and progressive neurodegeneration wi

71 n which the A(3) factors are not present and pre-rRNAs are unstable.

72 Nob1 binds before pre-rRNA cleavage, and we conclude that structural reorg

73 Physical links between pre-rRNA and these proteins were identified by co-immuno

74 in yeast Puf6 are important for RNA binding, pre-rRNA processing, and mRNA localization.

75 In puf6Delta or loc1Delta cells, pre-rRNA processing and 60S export are impaired and 60S

76 iates with small nucleolar RNPs to chaperone pre-rRNA processing and ribosome assembly.

77 t Rcl1, conserved in all eukaryotes, cleaves pre-rRNA at so-called site A(2), a co-transcriptional cl

78 Recombinant Rcl1 cleaves pre-rRNA mimics at site A(2) in a reaction that is sensi

79 subunit triggers its release from the common pre-rRNA transcript by stimulating cleavage at the proxi

80 inally, we review recent findings concerning pre-rRNA processing pathways and a novel mechanism invol

81 he Has1 DEAD-box RNA helicase in consecutive pre-rRNA processing and maturation steps for constructio

82 nd couples domain I folding with consecutive pre-rRNA processing steps.

83 ytoplasmic exonuclease Ngl2, and cytoplasmic pre-rRNA accumulated in strains lacking Ngl2.

84 The 5'-exonuclease Rat1 degrades pre-rRNA spacer fragments and processes the 5'-ends of t

85 rb1, destabilize the heterotrimer, and delay pre-rRNA processing and nuclear export of preribosomes.

86 RNA to 5.8S rRNA, leading to TRAMP-dependent pre-rRNA degradation.

87 NA and degradation of a variety of discarded pre-rRNA species.

88 nd the degradation of a variety of discarded pre-rRNA species.

89 that these enzymes are required for distinct pre-rRNA processing reactions leading to synthesis of 18

90 veals that despite the variants both driving pre-rRNA processing defects and 80S monosome reduction,

91 ing of snoRNAs and biogenesis factors during pre-rRNA processing, similar to its recycling role in pr

92 bosomal proteins and final cleavage of 18S-E pre-rRNA (18S-E).

93 10 and RPS26 showed elevated levels of 18S-E pre-rRNA.

94 U3 and Rrp9 are required for early pre-rRNA cleavages at sites A0, A1 and A2, but the mecha

95 RPS2B in atprmt3-2, which accounts for early pre-rRNA processing defects and results in nucleolar str

96 omplex, which seems to be primarily in early pre-rRNA processing and surveillance.

97 ncentrated in the nucleolus, where the early pre-rRNA processing reactions take place.

98 intact HAT domain is essential for efficient pre-rRNA processing and cell growth.

99 e activity of Grc3 is required for efficient pre-rRNA processing and that depletion of Grc3 leads to

100 in ncl-1 or lin-35/Rb, both of which elevate pre-rRNA levels.

101 nd pre-rRNA processing factors to elongating pre-rRNA on an as-needed basis rather than corecruitment

102 onstrate that the role of Rcl1 in eukaryotic pre-rRNA processing is identical to that of RNase III in

103 ed to RNase P, which functions in eukaryotic pre-rRNA processing.

104 tein endoribonuclease involved in eukaryotic pre-rRNA processing.

105 of all classes of RNA, and is essential for pre-rRNA processing.

106 The nuclear exosome is a key factor for pre-rRNA processing through the activity of its catalyti

107 erminal domain (CTD) of Rrp5 is required for pre-rRNA cleavage at sites A0-A2 on the pathway of 18S r

108 The 17 putative RNA helicases required for pre-rRNA processing are predicted to play a crucial role

109 box putative RNA helicases are required for pre-rRNA processing in Saccharomyces cerevisiae, althoug

110 helicase is required for snoRNA release from pre-rRNA and production of the U6 snRNP.

111 icase motifs of Has1p block U14 release from pre-rRNA.

112 ein inhibited the release of U14 snoRNA from pre-rRNA, just as was seen with Dbp4-depleted cells, ind

113 and promotes displacement of U8 snoRNA from pre-rRNA, which is necessary for the removal of the 3' e

114 rtant role in the release of U14 snoRNA from pre-rRNA.

115 d in the binding and release of snoRNPs from pre-rRNA.

116 Furthermore, pre-rRNAs are stable, indicating that the block in proce

117 Thus, a role for RNase MRP in human pre-rRNA processing is established.

118 tein (RNP) from HeLa cells cleaves the human pre-rRNA in vitro at at least one site used in cells, wh

119 Wild-type Prp43p immunoprecipitates pre-rRNAs and mature rRNAs, indicating a direct role in

120 sense oligonucleotides significantly impairs pre-rRNA processing in human cells.

121 l conserved eIF4A/eIF4G-like complex acts in pre-rRNA processing, adding to the established functions

122 g with dimethyl sulfate to detect changes in pre-rRNA structure upon genetic manipulation of the yeas

123 Mybbp1a protein levels results in defects in pre-rRNA processing within the cell.

124 been identified in proteins that function in pre-rRNA processing, including human Puf-A and yeast Puf

125 Deleting gamma2-AMPK led to increases in pre-rRNA level, ER stress markers, and cell death during

126 d in this process, many proteins involved in pre-rRNA processing and ribosomal subunit maturation hav

127 Specifically, proteins involved in pre-rRNA transcription, including subunits of the polyme

128 ucleotide modifications, some participate in pre-rRNA cleavages, and a few have both functions.

129 ts, and RNA sequencing-implicates MRP RNA in pre-rRNA processing.

130 aryotes, the enzyme performs a vital role in pre-rRNA processing in addition to its methylating activ

131 mplexes indicates this protein has a role in pre-rRNA processing.

132 The function of treacle in pre-rRNA methylation is most likely mediated by its dire

133 Unexpectedly, on many substrates, including pre-rRNAs and pre-mRNAs, binding specificity is apparent

134 isrupted nucleologenesis but did not inhibit pre-rRNA processing.

135 Depletion of Rrp14p inhibited pre-rRNA synthesis on both the 40S and 60S synthesis pat

136 , Nop12 and Nop15, which show interdependent pre-rRNA binding.

137 ls results in the appearance of intermediate pre-rRNAs species that reflect the processing of pre-rRN

138 mbly is a hierarchical process that involves pre-rRNA folding, modification, and cleavage and assembl

139 and nucleolar localization of the human ITS2 pre-rRNA endonuclease-kinase complex.

140 orm the internal transcribed spacer 2 (ITS2) pre-rRNA endonuclease-kinase machinery.

141 the nucleolar sub-structure linked with late pre-rRNA processing events.

142 e-rRNA) and to large ribosomal subunit (LSU) pre-rRNA processing independent of FANCD2.

143 hat BCCIP is a critical factor for mammalian pre-rRNA processing and 60S generation and offer an expl

144 ctor, thereby impairing exonuclease-mediated pre-rRNA processing and ribosome biogenesis in vascular

145 e how endonucleolytic cleavages of the mouse pre-rRNA transcript in the internal transcribed spacer 1

146 nuclease XRN2, a key coordinator of multiple pre-rRNA cleavages, driving mature rRNA formation and di

147 The mutant impairs processing of multiple pre-rRNA intermediates, resulting in the degradation of

148 leolar stress, as shown by decreased nascent pre-rRNA synthesis, fibrillarin perinucleolar cap format

149 elation between expression levels of nascent pre-rRNA and GPC5 (P = 0.001), but not a C13orf25 transc

150 me, can be observed at the 5' end of nascent pre-rRNA.

151 27kip1 response pathway and leads to nascent pre-rRNA reduction.

152 ch binding of a methyltransferase to nascent pre-rRNAs is a prerequisite to processing, so that all c

153 S rRNA base modifications but exhibit normal pre-rRNA processing.

154 by the nuclear exonuclease Rrp6, and nuclear pre-rRNA accumulated in the absence of Rrp6.

155 f the 35S pre-rRNA and cause accumulation of pre-rRNA species that normally have low steady-state lev

156 and is necessary for the full activation of pre-rRNA synthesis in vivo.

157 Amelioration of pre-rRNA imbalance is achieved through rescue of MRP RNA

158 ts demonstrate that quantitative analyses of pre-rRNA processing can yield important biological insig

159 orchestrate the modification and cleavage of pre-rRNA and are essential for ribosome biogenesis.

160 endo- and/or exo-ribonucleolytic cleavage of pre-rRNA remains unknown.

161 d nucleotides and participate in cleavage of pre-rRNA.

162 The subsequent downregulation of pre-rRNA level led to attenuated endoplasmic reticulum (

163 of the external transcribed spacer (ETS) of pre-rRNA.

164 d machinery that orchestrates the folding of pre-rRNA that results in the assembly of the small ribos

165 res modification, processing, and folding of pre-rRNA to yield mature rRNA.

166 ciate with preribosomes to enable folding of pre-rRNA, recruitment of ribosomal proteins, and process

167 re-rRNA and 5'-01, A0-1 and E-2 fragments of pre-rRNA transcript in the nucleolus.

168 es cerevisiae) and animal cells, hundreds of pre-rRNA processing factors have been identified and the

169 that MPA treatment results in inhibition of pre-rRNA synthesis and disruption of the nucleolus.

170 lar disassembly occurs without inhibition of pre-rRNA transcription, a well known trigger for nucleol

171 Recent results indicate that levels of pre-rRNA have prognostic value and that a tRNA has oncog

172 s laevis oocytes reduced 2'-O-methylation of pre-rRNA.

173 pation of treacle in the 2'-O-methylation of pre-rRNA.

174 ion and post-transcriptional modification of pre-rRNA.

175 We propose that probing of pre-rRNA maturation intermediates by exonucleases serves

176 In addition, NS regulates processing of pre-rRNA and consequently the level of total protein syn

177 nthesis: the transcription and processing of pre-rRNA and the transcription of ribosomal protein gene

178 (45S rDNA), cotranscriptional processing of pre-rRNA, and assembly of mature rRNA with ribosomal pro

179 essential participants in the processing of pre-rRNA.

180 ns, as well as Nob1-dependent protections of pre-rRNA in vitro and in vivo demonstrate that Nob1's bi

181 Indeed, quantification of pre-rRNA levels indicated ongoing rDNA transcription in

182 ngation, resulting in a drastic reduction of pre-rRNA and pre-tRNA synthesis, the disruption of the n

183 confirmed SIRT1's role in the regulation of pre-rRNA synthesis and processing.

184 er, many protein effectors and regulators of pre-rRNA processing needed for rRNA maturation were also

185 Assembly of eNoSC facilitated repression of pre-rRNA transcription.

186 d and annotated by means of a graphic set of pre-rRNA ratios, a technique we call Ratio Analysis of M

187 We also find that splitting of pre-rRNA in the 3' region of ITS1 is prevalent in adult

188 s suggest that CARF regulates early steps of pre-rRNA processing during ribosome biogenesis by contro

189 ct that is transcribed, whereas subgroups of pre-rRNA processing factors are recruited to plasmids on

190 nucleoplasm and a concomitant suppression of pre-rRNA processing that leads to accumulation of the 5'

191 se can switch from processing to turnover of pre-rRNA.

192 n 90S but also in site 2 cleavage in ITS1 of pre-rRNAs at early stages of human ribosome biogenesis;

193 rRNAs species that reflect the processing of pre-rRNAs through Pathway 2, a pathway that processes pr

194 ent of ribosomal proteins, and processing of pre-rRNAs to produce mature ribosomes.

195 es the coordinated folding and processing of pre-rRNAs with assembly of ribosomal proteins.

196 17, L35 and L37 in folding and processing of pre-rRNAs, and binding of other proteins within assembli

197 ase Prp43 in the 18S and 25S rRNA regions of pre-rRNAs, using UV crosslinking.

198 t requires the endonucleolytic separation of pre-rRNAs to initiate rRNA production.

199 A subset of pre-rRNAs accumulates when the SEN complex is restricted

200 We map DDX21 crosslinking sites on pre-rRNAs and show their overlap with the basepairing si

201 mponent of ribosome biogenesis, particularly pre-rRNA processing.

202 hat copy numbers of ribosomal-RNA precursor (pre-rRNA) of specific pathogen species relative to genom

203 mal particles containing 35S rRNA precursor (pre-rRNA).

204 o establish domain I architecture to prevent pre-rRNA turnover and couples domain I folding with cons

205 ires multiple nuclease activities to process pre-rRNA transcripts into mature rRNA species and elimin

206 to phosphorylate Tif6p was unable to process pre-rRNAs efficiently, resulting in significant reductio

207 through Pathway 2, a pathway that processes pre-rRNAs in a different temporal order than the more of

208 Las1 plays a fundamental role in processing pre-rRNA.

209 We focused on L-Myc, which promoted pre-rRNA synthesis and transcriptional programs associat

210 mal RNAs (pre-rRNAs) and ribosomal proteins, pre-rRNA processing, and subunit assembly with the aid o

211 ture is not required for the nucleolin RBD12/pre-rRNA NRE interaction.

212 of the functional organization of 45S rDNA, pre-rRNA transcription, rRNA processing activities, and

213 pUL31 is necessary and sufficient to reduce pre-rRNA levels, and this was dependent on the dUTPase-l

214 arginine methyltransferase AtPRMT3 regulates pre-rRNA processing; however, the underlying molecular m

215 Furthermore, depletion of Xrn2 reveals pre-rRNAs derived by cleavage events that deviate from t

216 inking identified numerous preribosomal RNA (pre-rRNA) binding sites for the large, highly conserved

217 d to the transcription of pre-ribosomal RNA (pre-rRNA) and to large ribosomal subunit (LSU) pre-rRNA

218 nucleolar RNA (snoRNA) on pre-ribosomal RNA (pre-rRNA) is essential for rRNA processing to produce 18

219 role in processing precursor ribosomal RNA (pre-rRNA) is largely unclear.

220 iogenesis, functioning in pre-ribosomal RNA (pre-rRNA) processing as a component of the small ribosom

221 d analysis of human precursor ribosomal RNA (pre-rRNA) processing because surprisingly little is know

222 to multiple sites in the pre-ribosomal RNA (pre-rRNA) to promote early cleavage and folding events.

223 procedure that minimizes pre-ribosomal RNA (pre-rRNA) transcripts.

224 muscles of mice without NO66, ribosomal RNA, pre-rRNA, and protein synthesis all increased.

225 oordinated expression of pre-ribosomal RNAs (pre-rRNAs) and ribosomal proteins, pre-rRNA processing,

226 Pre-ribosomal RNAs (pre-rRNAs) must be processed stepwise and at the correct

227 ribosomal proteins (RPLs) in precursor rRNA (pre-rRNA) processing correlates with their location in t

228 ot strictly required for the precursor rRNA (pre-rRNA) processing reactions but contributes to optima

229 ve been implicated in the processing of 20 S pre-rRNA and are necessary for survival of the cells.

230 f nucleostemin delays the processing of 32 S pre-rRNA into 28 S rRNA.

231 ignificantly promotes the processing of 32 S pre-rRNA.

232 e it is essential for the processing of 35 S pre-rRNA to the mature 25 S and 5.8 S rRNAs.

233 or conversion of 27SA(3) pre-rRNA to 27SB(S) pre-rRNA.

234 g reactions but contributes to optimal 27 SB pre-rRNA maturation.

235 emia, we detected no evidence of significant pre-rRNA processing disturbance in cells derived from tw

236 are recruited to plasmids only when specific pre-rRNA fragments are produced.

237 uding transcription, editing, mRNA splicing, pre-rRNA processing, RNA transport and RNA decay, scanni

238 Our study demonstrates that steady-state pre-rRNA-analysis can be a valuable culture-independent

239 The method, so-called steady-state pre-rRNA-analysis, represents a novel culture-independen

240 RNases recognize and process double-stranded pre-rRNA.

241 The cKO failed to suppress pre-rRNA level during ischemia/reperfusion and showed a

242 PK translocates into the nucleus to suppress pre-rRNA transcription and ribosome biosynthesis during

243 ecreased H3K4me3 and H3K36me3 and suppressed pre-rRNA expression.

244 after EX-527 treatment with the outcome that pre-rRNA and 28S rRNA levels also increased.

245 ealed multiple Rat1-binding sites across the pre-rRNA, consistent with its known functions.

246 endonucleolytic cleavage in ITS1, binds the pre-rRNA near the 5'-end of 5.8S.

247 be the elusive endonuclease that cleaves the pre-rRNA at sites A(1) and/or A(2.).

248 eins act as chaperones to correctly fold the pre-rRNA substrates and, for Mini-III, anchor the RNase

249 /RHA RNA helicase Dhr1 dislodges U3 from the pre-rRNA.

250 s likely unbound by Nob1 and flexible in the pre-rRNA.

251 impairs the early cleavage reactions of the pre-rRNA and causes U14 small nucleolar RNA (snoRNA) to

252 st and leads to defects in processing of the pre-rRNA internal transcribed spacer 2 region.

253 ubstitution of the 5'-ETS nucleotides of the pre-rRNA involved in this initial base pairing interacti

254 rently high evolutionary conservation of the pre-rRNA processing pathway and ribosome synthesis facto

255 avages in the 5'-ETS and ITS2 regions of the pre-rRNA.

256 I from the rDNA and reduced synthesis of the pre-rRNA.

257 mation and cotranscriptional cleavage of the pre-rRNA.

258 he small subunit processome to dock onto the pre-rRNA, an event indispensable for ribosome biogenesis

259 uppressed polyadenylation and stabilized the pre-rRNA and rRNA.

260 vivo RNA structure probing revealed that the pre-rRNA processing defects are due to misfolding of 5.8

261 Dhr1 was cross-linked in vivo to the pre-rRNA and to U3 sequences flanking regions that base-

262 ences flanking regions that base-pair to the pre-rRNA including those that form the CPK.

263 Binding of the U3 box A region to the pre-rRNA is mutually exclusive with folding of the centr

264 Notably, variation is not restricted to the pre-rRNA sequences removed during processing, but it is

265 al anchor that recruits the U3 snoRNA to the pre-rRNA, is a prerequisite for the subsequent interacti

266 te that it may function to bring Dbp8 to the pre-rRNA, thereby both regulating its enzymatic activity

267 me biogenesis, U3 snoRNA base pairs with the pre-rRNA to promote its processing.

268 cteria: to co-transcriptionally separate the pre-rRNAs destined for the small and large subunit.

269 tures of these RNases poised to cleave their pre-rRNA substrates within the B. subtilis 50S particle.

270 9 different RNA helicases, but none of their pre-rRNA-binding sites were previously known, making the

271 kely plays a pivotal role in remodeling this pre-rRNA region in both yeast and humans.

272 24 is essential for early cleavages at three pre-rRNA sites in yeast (A0, A1 and A2) and humans (A0,

273 how structural rearrangements are coupled to pre-rRNA processing are not understood.

274 Nob1 forms a tetramer that binds directly to pre-rRNA analogs containing cleavage site D.

275 snoRNA), an essential RNA that base pairs to pre-rRNA.

276 This complex contains proteins related to pre-rRNA processing, such as Pes1, DDX21, and EBP2, in a

277 We show that Esf2 can bind to pre-rRNAs and speculate that it may function to bring Db

278 RPS2B binds directly to pre-rRNAs in the nucleus, and such binding is enhanced i

279 oli YbeY protein: an endonuclease that trims pre-rRNAs to their mature forms and a sentinel that part

280 ts mature structure because it contained U3, pre-rRNA, and a number of early-acting ribosome synthesi

281 By mediating formation of both essential U3-pre-rRNA duplexes, Imp3p and Imp4p may help the small su

282 Imp3p, and Imp4p as candidates to mediate U3-pre-rRNA interactions.

283 propose that the Rrp9 beta-propeller and U3/pre-rRNA binding cooperate in the structure or stability

284 with mutations in U3 that destabilize the U3/pre-rRNA base-pair interactions or reduce the length of

285 d discuss its implications for understanding pre-rRNA processing pathways.

286 external transcribed spacer from unprocessed pre-rRNA and for processing the 3' tail of snRNA U4.

287 Rio1-associated pre-40S particles, in vitro pre-rRNA cleavage was strongly stimulated by ATP and req

288 ltered the structure of the nucleolus, where pre-rRNA processing occurs.

289 precipitation that complexes associated with pre-rRNA processing factors are ubiquitinated.

290 eolar RNA (snoRNA) to remain associated with pre-rRNA.

291 The production of risiRNAs correlates with pre-rRNA processing defects in these mutants.

292 interact directly with MRP RNA and four with pre-rRNA.

293 ated manner and that their interactions with pre-rRNA could be coupled.

294 ol I transcription factor UBF interacts with pre-rRNA processing factors as analyzed by immunoprecipi

295 volve direct base-pairing of the snoRNA with pre-rRNA using different domains.

296 al interactions of many factors/snoRNAs with pre-rRNA for correct rRNA processing and ribosome assemb

297 ation of RNA polymerase I transcription with pre-rRNA processing, preribosomal particle assembly, and

298 Overlapping basepairing of snoRNAs with pre-rRNAs often necessitates sequential and efficient as

299 previous reports that modification of yeast pre-rRNA exclusively occurred on released transcripts.

300 ance in the 40S:60S free subunits ratio; yet pre-rRNA processing appeared to occur normally.