ライフサイエンスコーパス: pre-tRNA

1 may have developed novel alternatives to 5' pre-tRNA processing.

2 ic rate constants for pre-tRNA(met608) and a pre-tRNA(met608) (+1)C/(+72)A mutant provides evidence t

3 scripts from such genes must be cleaved by a pre-tRNA endonuclease to form a functional tRNA.

4 displayed by the protein specifically for a pre-tRNA substrate.

5 gnize and cleave any target RNA that forms a pre-tRNA-like complex with another RNA, in some cases cl

6 he highly structured T stem-loop region in a pre-tRNA substrate is bound by the B. subtilis P RNA.

7 Individual modifications in a pre-tRNA substrate that disrupt ES interactions proximal

8 e S-domain binds the T stem-loop region in a pre-tRNA substrate to confer specificity for tRNA substr

9 get mRNA to form a tertiary structure like a pre-tRNA and recruit intracellular ribonuclease P (RNase

10 imately 4-fold better than the cleavage of a pre-tRNA substrate by the wild-type RNase P RNA under th

11 The 2'OH group at the cleavage site of a pre-tRNA substrate is an important determinant in the cl

12 tional groups in the T stem-loop region of a pre-tRNA substrate previously identified to directly con

13 that the C terminus is required to protect a pre-tRNA anticodon stem from chemical modification.

14 ubstrate selectivity is investigated using a pre-tRNA substrate containing single-atom modifications

15 Soaks with a pre-tRNA 5' leader sequence with and without metal help

16 nzyme reaction is much more efficient with a pre-tRNA substrate, binding at least 100-fold better and

17 bonucleoprotein enzyme RNase P processes all pre-tRNAs, yet some substrates apparently lack consensus

18 trate that is cleaved slowly by P RNA alone (pre-tRNA(f-met605)) and one that is cleaved quickly (pre

19 ain cells and mass, with evidence of altered pre-tRNA processing.

20 re cleaved only after the discriminator, and pre-tRNA variants with a total of N bp (N is less than 1

21 icated to be important for tRNA identity and pre-tRNA processing.

22 d at specific locations on the P protein and pre-tRNA 5' leader.

23 uence-specific contact between P protein and pre-tRNA that contributes to molecular recognition of RN

24 cross-linked nucleotides in the ribozyme and pre-tRNA were determined by reverse transcriptase primer

25 s, while tRNA transcription and pre-rRNA and pre-tRNA cleavage processing appear normal.

26 lting in a drastic reduction of pre-rRNA and pre-tRNA synthesis, the disruption of the nucleolus, and

27 affinity purification-mass spectrometry and pre-tRNA cleavage assays of MOB3C pulldowns.

28 gene, was copied into the acceptor stem and pre-tRNA region.

29 ed box C/D RNPs positioned in either another pre-tRNA(Trp) or in the excised intron.

30 olved in tRNA expression/function as well as pre-tRNA splicing.

31 cent RNA polymerase III transcripts, such as pre-tRNAs, whereas in the cytoplasm they contribute to t

32 La has also been hypothesized to assist pre-tRNAs in attaining their native fold through RNA cha

33 a and dysfunctional processing of associated pre-tRNAs that are spliced but 5' and 3' unprocessed, wi

34 he ability to cleave the covalently attached pre-tRNA, indicating that the cross-links reflect the na

35 ree of structural changes from the authentic pre-tRNA.

36 Both av pre-tRNAs are able to fold into two conformations: 1 and

37 n a scenario elucidated by the use of the av pre-tRNAs, algal permuted tRNA genes could have further

38 ting of a small group I intron from Azoarcus pre-tRNA(Ile) showed that tertiary interactions between

39 te of a small group I ribozyme from Azoarcus pre-tRNA(ile).

40 that both tRNA(Gln) species and a bacterial pre-tRNA(Asp) can be imported in vitro into mitochondria

41 f1 via small interfering RNA increased basal pre-tRNA and rendered tRNA synthesis refractory to mTOR

42 rry out two cis-methylation reactions before pre-tRNA splicing.

43 These findings demonstrate a link between pre-tRNA splicing and pre-mRNA 3' end formation, suggest

44 splays a 3-fold greater affinity for binding pre-tRNA substrates relative to tRNA products.

45 , it becomes protease-resistant upon binding pre-tRNAs, U6 RNA, or pre-5S rRNA.

46 Herein we describe the use of biotinylated pre-tRNA substrates to isolate RNase P ribozyme-substrat

47 those with the rna1-1 mutation, affect both pre-tRNA splicing and RNA export.

48 SEN2 exhibited defects in maturation of both pre-tRNA and pre-mRNA.

49 r cytoplasmic recruitment, packaging of both pre-tRNAs and U6 snRNA requires the nuclear export recep

50 component of bacterial RNase P can catalyse pre-tRNA cleavage in the absence of the RNase P protein

51 ecause some early steps in the S. cerevisiae pre-tRNA biosynthetic pathway are nucleolar, we examined

52 the rate constant for catalysis for certain pre-tRNA substrates up to 1000-fold.

53 is required for efficient folding of certain pre-tRNAs.

54 to the catalytic center, to bind and cleave pre-tRNA.

55 gomeric states of Hth1307 are able to cleave pre-tRNAs.

56 inetic studies indicate that Hth1307 cleaves pre-tRNAs from multiple species with a preference for na

57 ities and cleavage rates of Escherichia coli pre-tRNAs that exhibit the largest variation from consen

58 ly characterized substrate-enzyme conjugate [pre-tRNA(Tyr)-Methanocaldococcus jannaschii (Mja) RPR] t

59 s of cleavage of non-consensus and consensus pre-tRNAs.

60 (SEN) during processing of intron-containing pre-tRNAs and by Ire1 cleavage of HAC1 mRNA following in

61 Both intron-containing pre-tRNAs and spliced tRNAs, regardless of whether they

62 studies of the location of intron-containing pre-tRNAs in the rna1-1 mutant rule out the possibility

63 xport of newly transcribed intron-containing pre-tRNAs.

64 rom postmitotic neurons results in defective pre-tRNA and pre-rRNA processing and progressive neurode

65 pathways for tRNA maturation, with defective pre-tRNAs being most sensitive to decay and most depende

66 d nuclear accumulation, prevented disordered pre-tRNA processing, and restored suppression, indicatin

67 tRNase Z(L) processes a nuclear-encoded pre-tRNA approximately 1600-fold more efficiently than t

68 ss both nuclear- and mitochondrially encoded pre-tRNAs.

69 ased enzyme primarily responsible for 5'-end pre-tRNA processing.

70 cleavage of precursor tRNA in vivo, enhances pre-tRNA binding by directly contacting the 5'-leader se

71 hylation of the two nucleotides of exogenous pre-tRNA(Trp) added to an H. volcanii cell extract also

72 Aberrant 3'-extended pre-tRNAs were detected, presumably due to stabilization

73 p in assisting formation of correctly folded pre-tRNA anticodon stems in vivo.

74 . subtilis RNase P has a higher affinity for pre-tRNA with adenosine at N(-4), and this binding prefe

75 igase follows the same chemical steps as for pre-tRNA splicing.

76 ers the structural and mechanistic basis for pre-tRNA processing by the prokaryotic HARP system.

77 nce of apparent catalytic rate constants for pre-tRNA(met608) and a pre-tRNA(met608) (+1)C/(+72)A mut

78 competes with the 3' -> 5' exonucleases for pre-tRNA substrates adding short poly(A) tails, which no

79 k(cat)/K(m) (by approximately 360-fold) for pre-tRNA cleavage to those observed with partially purif

80 We propose a model for pre-tRNA cleavage in which an essential Mg2+ ion is coor

81 etal ion stabilizes the transition state for pre-tRNA cleavage.

82 one site stabilizes the transition state for pre-tRNA cleavage.

83 equence-specific contacts between the fourth pre-tRNA nucleotide on the 5' side of the cleavage site

84 s, the enzyme catalyzing intron removal from pre-tRNA is a heterotetrameric complex (splicing endonuc

85 s the removal of the 5'-leader sequence from pre-tRNA to produce the mature 5' terminus.

86 lyzes removal of the 5'-leader sequence from pre-tRNAs with its NYN metallonuclease domain.

87 clease P cleaves 5'-precursor sequences from pre-tRNAs.

88 uclease (3' tRNase) removes 3' trailers from pre-tRNAs by cleaving the RNA immediately downstream of

89 s that perform the same biological function, pre-tRNA maturation, thereby providing insight into the

90 In general, pre-tRNA variants containing a total of more than 11 bp

91 ly processed endonucleolytically to generate pre-tRNA species, which undergo 5'-end maturation by the

92 within polycistronic transcripts to generate pre-tRNAs that subsequently become substrates for RNase

93 i RPR's cis cleavage of precursor tRNA(Gln) (pre-tRNA(Gln)), which lacks certain consensus structures

94 On the other hand, pre-tRNA variants containing extra acceptor stem base-pa

95 er relative to the protein in the holoenzyme-pre-tRNA complex.

96 Recent work has demonstrated that the host pre-tRNA processing enzyme, RNase P, can cleave the HCV

97 yadenylation and degradation of hypomodified pre-tRNA(i)(Met).

98 The conformation of a group I pre-tRNA(ile) from the bacterium Azoarcus was probed by

99 SEN) was destabilized, resulting in impaired pre-tRNA cleavage.

100 The impaired pre-tRNA processing seen on Lsm depletion is not, howeve

101 ity is accompanied by a 500-fold decrease in pre-tRNA cleavage efficiency (k(cat)/K(M)).

102 ct tRNA nuclear export also cause defects in pre-tRNA splicing leading to tight coupling of the splic

103 lear compartmentalization causing defects in pre-tRNA splicing.

104 RNase P protein is to offset differences in pre-tRNA structure such that binding and catalysis are u

105 ests that some aspects of their functions in pre-tRNA and pre-rRNA processing pathways might overlap

106 tin-t homologue, binds tRNA and functions in pre-tRNA splicing and export of mature tRNA from the nuc

107 y conserved regions, the regions involved in pre-tRNA recognition and the location of the active site

108 To understand the role of Los1p in pre-tRNA splicing, we sought los1 multicopy suppressors.

109 A mutation in pre-tRNA(Arg)(CCG) causes yeast cells to be cold-sensiti

110 portin for tRNA, suppresses the reduction in pre-tRNA levels, AAM gene up-regulation, and slow growth

111 8 surrounding the natural processing site in pre-tRNA substrates.

112 fication and cleavage of the splice sites in pre-tRNA.

113 An early step in pre-tRNA maturation is removal of the 5' leader by the e

114 licing of tRNA introns is a critical step in pre-tRNA maturation.

115 , and J18/2 regions of ribozyme structure in pre-tRNA binding and implicate an additional region, J11

116 catalyzes essential removal of 5' leaders in pre-tRNAs.

117 ecursors involves separation into individual pre-tRNAs by one of several ribonucleases followed by 5'

118 Nonphosphorylated La (npLa) inhibits pre-tRNA processing, while phosphorylation of human La s

119 Puromycin inhibits pre-tRNA processing by the Tetrahymena complex, and impl

120 lencing, suggesting that synthesis of intact pre-tRNAs is required for the silencing mechanism.

121 Considering that perturbed intranuclear pre-tRNA metabolism and apparent deficiency in tRNA nucl

122 and m(1)A within mitochondrial Ile and Leu1 pre-tRNA regions, respectively, in nascent polycistronic

123 ific C-terminal domain (CTD) of hLa maintain pre-tRNA in an unprocessed state by blocking the 5'-proc

124 o 5' processing of nuclear and mitochondrial pre-tRNAs occurs before 3' processing.

125 es accumulation of nuclear and mitochondrial pre-tRNAs, suggesting that JhI-1 encodes both forms of t

126 We used two avatar (av) or model pre-tRNAs and two splicing endonucleases with distinct m

127 The model pre-tRNAs permit description of the features that a tran

128 leader sequence of precursor tRNA molecules (pre-tRNA), whereas the protein subunit assists in substr

129 Consistently, overexpression of a mutant pre-tRNA(Tyr) that cannot be processed by RNase P had a

130 Although maturation of the mutant pre-tRNA(Ser)CGA requires Lhp1p, introduction of a secon

131 t deficiency in the processing of the mutant pre-tRNA, that becomes limiting for protein synthesis on

132 s could affect tRNase Z processing of mutant pre-tRNAs, perhaps contributing to mitochondrial disease

133 Previous kinetic analyses with mutant pre-tRNAs indicated that both C residues of the invarian

134 f La with the 5' triphosphate end of nascent pre-tRNA.

135 Nascent pre-tRNAs released from a terminator by C37 mutants have

136 The nascent pre-tRNAs must undergo folding, 5' and 3' processing to

137 experiments showed that formation of native pre-tRNA is delayed by misfolding of P3-P9, including mi

138 equences form separate domains in the native pre-tRNA, their folding is coupled via metastable non-na

139 better than the EGS generated from a natural pre-tRNA.

140 n subunit to catalysis for some nonconsensus pre-tRNAs.

141 However, nuclear pre-tRNA splicing fails to complement growth defects of

142 In addition, Mod5 is bound to nuclear pre-tRNA transcripts, although they are not substrates f

143 Comparative analyses of pre-tRNA and tRNA binding to the RNase P holoenzyme and

144 hat facilitates the efficient association of pre-tRNA processing factors with their substrates.

145 basic region are required for attenuation of pre-tRNA 5' processing.

146 and in bacteria the RNA alone is capable of pre-tRNA processing in vitro, i.e. it is a catalytic RNA

147 The catalysis of pre-tRNA by the eukaryotic tRNA-splicing endonuclease th

148 We conclude that the 3'-CCA of pre-tRNA, particularly the 3'-proximal C residue, compri

149 The single turnover pH dependence of pre-tRNA cleavage revealed a single ionization (pKa appr

150 protein component alter the pH dependence of pre-tRNA(Asp) cleavage catalyzed by RNase P, providing f

151 RNA processing, with altered distribution of pre-tRNA intermediates.

152 of contact between the mature tRNA domain of pre-tRNA and the ribozyme; however, relatively little is

153 haalphabetabeta hand that binds the elbow of pre-tRNA.

154 es not affect interaction with the 3' end of pre-tRNA.

155 III transcription with the nuclear export of pre-tRNA.

156 We investigated what features of pre-tRNA determine the cleavage site using various pre-t

157 and ts-44 are derived from distinct genes of pre-tRNA(His), and are down-regulated in CLL 3- to 5-fol

158 nonical editing events, within the intron of pre-tRNA(Tyr)GUA, involving guanosine-to-adenosine trans

159 These data demonstrate that the 5' leader of pre-tRNA moves 4 to 6 A closer to the PRNA x P protein i

160 irs with the 5' leader increases the K(m) of pre-tRNA slightly, in agreement with previous results.

161 exhibit higher fidelity of 5'-maturation of pre-tRNA(Gln) and some of its mutant derivatives.

162 ently been shown to facilitate maturation of pre-tRNA, and three distinct ribosomal proteins, Rpl16p,

163 Measurements of pre-tRNA-containing adenosine analogs at N(-4) indicate

164 re we investigate the enzymatic mechanism of pre-tRNA hydrolysis catalyzed by the NYN (Nedd4-BP1, Yac

165 bases encoding the +1 and +2 nucleotides of pre-tRNA Tyr, had a strong deleterious effect in vivo, a

166 zation is required for the normal pathway of pre-tRNA maturation, facilitates assembly of small RNAs

167 that operates upstream of a major pathway of pre-tRNA maturation, which itself is upstream of tRNA ex

168 Although processing of pre-tRNA(i)(Met) and other tRNA precursors, and the amin

169 0-fold decrease in the self-cleavage rate of pre-tRNA(Tyr)-MjaDeltaU RPR compared to the wild type, a

170 t in RNase P is to facilitate recognition of pre-tRNA by enhancing the interaction between the enzyme

171 c endonuclease that catalyzes the removal of pre-tRNA leader sequences to form the 5' end of mature t

172 cleavage by Escherichia coli RNase P RNA of pre-tRNA in which specific pro-Rp phosphate oxygens were

173 th the single-stranded 5' leader sequence of pre-tRNA, and (ii) the orientation and register of the p

174 photoagent was incorporated into a series of pre-tRNA substrates containing unique uridine residues i

175 The mutation causes the anticodon stem of pre-tRNA(Arg)(CCG) to misfold into an alternative helix

176 logy and demonstrate that all three steps of pre-tRNA splicing, as well as tRNA nuclear export and am

177 ates, and determined the first structures of pre-tRNA-bound human TSEN complexes.

178 nism of HAC1 mRNA splicing resembles that of pre-tRNA splicing.

179 nuclease protection assays with a variety of pre-tRNA substrates and mutant La proteins indicate that

180 he helicase motif alters the accumulation of pre-tRNAs, pre-rRNAs, and some small nuclear RNAs.

181 is responsible for processing the 5' end of pre-tRNAs as well as other RNA molecules.

182 aves precursor sequences from the 5' ends of pre-tRNAs.

183 eal that a greater-than-expected fraction of pre-tRNAs from both E. coli and B. subtilis contains a n

184 d a marked decline in the cellular levels of pre-tRNAs.

185 domain and the anticodon-intron base pair of pre-tRNAs.

186 pathway that monitors both end processing of pre-tRNAs and the modification state of mature tRNAs.

187 a protein can also modulate 5' processing of pre-tRNAs.

188 protein subunit and the leader sequences of pre-tRNAs may be common in bacterial RNase P and may lea

189 tes that bind and protect the 3' trailers of pre-tRNAs from exonuclease digestion via sequence-specif

190 the 5' leader sequences had little effect on pre-tRNA folding.

191 ation enzymes, have unanticipated effects on pre-tRNA end processing.

192 ds in the 3' --> 5' direction generating one pre-tRNA at a time.

193 for the wild-type pre-tRNA(Ser)CGA and other pre-tRNAs, Lhp1p is required for the normal endonucleoly

194 of "corrected" tRNAs from such a "permuted" pre-tRNA configuration.

195 omplex indirectly by binding and positioning pre-tRNA.

196 Eukaryotic precursor (pre)-tRNAs are processed at both ends prior to maturatio

197 Introns of human transfer RNA precursors (pre-tRNAs) are excised by the tRNA splicing endonuclease

198 Eukaryotic transfer RNA precursors (pre-tRNAs) contain a 5' leader preceding the aminoacyl a

199 denylated Pol III transcripts, predominately pre-tRNAs.

200 n-coding RNA, snoRNAs and, most prominently, pre-tRNAs and other Pol III transcripts are targeted for

201 ion of human La serine-366 (S(366)) promotes pre-tRNA processing.

202 (f-met605)) and one that is cleaved quickly (pre-tRNA(met608)) pinpoint the characteristic C(+1)/A(+7

203 endonuclease (TSEN) complex, largely reduced pre-tRNA cleavage activity, and accumulation of linear t

204 endent polycistronic tRNA operons to release pre-tRNAs is the essential function of the enzyme, since

205 length and that this significantly restricts pre-tRNAs to a La-independent pathway of maturation in f

206 of the leader to 2 nucleotides due to P RNA-pre-tRNA contacts that are stabilized by the P protein.

207 Select pre-tRNAs and mature tRNAs with PR and POLR3A colocalize

208 RNA mutations: a mutation can impede several pre-tRNA processing steps, with each such reduction cont

209 A small pre-tRNA (R-ATW) composed of an acceptor stem, an extra

210 ts for 3' tRNase substrates, we tested small pre-tRNA(Arg) substrates lacking the D and anticodon ste

211 on elicits increased polyadenylation of some pre-tRNAs by poly(A) polymerase I (PAP I), which exacerb

212 that La is stably associated with a spliced pre-tRNA intermediate.

213 at least four magnesium sites that stabilize pre-tRNA binding and, possibly, catalysis.

214 We propose that binding by Lhp1p stabilizes pre-tRNAs in conformations that allow the 3' endonucleol

215 molecule which by itself can bind substrate pre-tRNA, select and hydrolyze the correct phosphodieste

216 ribonuclease P (RNase P) binds to substrate pre-tRNAs with high affinity and catalyzes site-specific

217 5I E478K and E478K) bound the completed SUP4 pre-tRNA more avidly.

218 Suppressor pre-tRNA(Ser)UCA-C47:6U with a debilitating substitution

219 Here, we demonstrate that pre-tRNA binding affinities for Bacillus subtilis and Es

220 d guide nucleotides, we now demonstrate that pre-tRNA methylation is guided in trans by the intron-en

221 results are consistent with the notion that pre-tRNAs recruit RNAP II-associated factors, thereby re

222 These experiments reveal that pre-tRNAs can require protein assistance for efficient f

223 nfected cells expressing the variant and the pre-tRNA-derived EGS, respectively.

224 thylation-competent box C/D RNPs on both the pre-tRNA and the excised intron (both linear and circula

225 The 3'-end trailer can be removed by the pre-tRNA processing endonuclease tRNase Z, an ancient, c

226 activity is dependent on 3' oligo(U) in the pre-tRNA for interaction with the N-terminal RNA binding

227 the first position in the palindrome in the pre-tRNA sequence, which does not affect tRNA function.

228 In the pre-tRNA, Tb(3+) cleavage was redirected to the 5' and 3

229 s part of the catalytic pocket formed in the pre-tRNA-RNase P complex and participates in the binding

230 a1p corrects the nuclear export, but not the pre-tRNA-splicing defects of los1Kan(r) cells, thereby u

231 roteins to determine the conformation of the pre-tRNA 5' leader relative to the protein in the holoen

232 acts with N(-4) and N(-5) nucleotides of the pre-tRNA 5'-leader.

233 separate the 5' from the 3' terminus of the pre-tRNA and to position the cleavage site in the cataly

234 auses partial or complete degradation of the pre-tRNA by RNase R, whereas extension of the stem resul

235 and (ii) the orientation and register of the pre-tRNA leader sequence in the central cleft places the

236 cal data and three-dimensional models of the pre-tRNA showed that the tRNA is folded, and that the tR

237 t -2 and -5 in the 5' leader sequence of the pre-tRNA substrate.

238 o recognize and bind the elbow region of the pre-tRNA substrate.

239 so eliminate the obligatory refolding of the pre-tRNA that would be required to carry out two cis-met

240 ed region, likely alters the geometry of the pre-tRNA-binding cleft and may provide a functional expl

241 th distinct mechanisms of recognition of the pre-tRNA.

242 methyltransferase subcomplex recognises the pre-tRNA in a distinct mode that can support tRNA-end pr

243 A uses a specificity module to recognize the pre-tRNA and a catalytic module to perform cleavage.

244 mase family, endonucleolytically removes the pre-tRNA 3' trailer in a step central to tRNA maturation

245 Kinetic assays showed that the pre-tRNA folds in minutes, much more slowly than the int

246 ribozyme that are in close proximity to the pre-tRNA cleavage site, we introduced the short-range ph

247 feedback" of nucleus/cytosol exchange to the pre-tRNA splicing machinery.

248 ow the conserved nucleotides adjacent to the pre-tRNA substrate contribute to substrate binding and p

249 ing that binding of these antibiotics to the pre-tRNA substrate contributes to the inhibitory activit

250 formations of the two 3 nt bulges, where the pre-tRNA is cleaved, are stabilized by stacking interact

251 for tgm silencing regardless of whether the pre-tRNA transcripts are substrates for Mod5 modificatio

252 Nucleoli were slightly fragmented, and the pre-tRNAs went from their normal, mostly nucleolar locat

253 which not only modulate the stability of the pre-tRNAs, but also regulate the availability of functio

254 box C/D guide RNA within the intron of this pre-tRNA led to the assumption that nucleotide methylati

255 y also detects binding of small molecules to pre-tRNA.

256 e NRE and RRM1 affect binding of human La to pre-tRNAs but not UUU-3'OH or poly(A) sequences, and we

257 ugh binding by the yeast La protein Lhp1p to pre-tRNAs is required for the normal pathway of tRNA mat

258 intron that must be removed from transcribed pre-tRNAs to generate mature, functional tRNAs.

259 r sequences from tRNA precursor transcripts (pre-tRNAs) by ribonuclease P (RNase P) is essential for

260 , is the RNA subunit of RNase P, which trims pre-tRNA transcript 5' ends.

261 he cleavage of the 5' end of precursor tRNA (pre-tRNA) catalyzed by ribonuclease P (RNase P).

262 nhancing the affinity of the precursor tRNA (pre-tRNA) substrate.

263 a catalytic RNA that cleaves precursor tRNA (pre-tRNA) to form the mature 5'-end of tRNA.

264 iscriminator nucleotide from precursor tRNA (pre-tRNA).

265 zes the 5' end maturation of precursor tRNA (pre-tRNA).

266 to cleave the 5' leader of precursor tRNAs (pre-tRNAs) and generate mature tRNAs.

267 its PPR domain to recognize precursor tRNAs (pre-tRNAs) as it catalyzes removal of the 5'-leader sequ

268 moval of the 5' leader from precursor tRNAs (pre-tRNAs) in all three domains of life.

269 e of the leader sequence of precursor tRNAs (pre-tRNAs), generating the mature 5' end of tRNAs.

270 of 5' leader sequences from precursor tRNAs (pre-tRNAs).

271 avage of the 5'-leader from precursor tRNAs (pre-tRNAs).

272 es the 5'-end maturation of precursor tRNAs (pre-tRNAs).

273 catalyzes 5'-maturation of precursor tRNAs (pre-tRNAs).

274 We demonstrate that for the wild-type pre-tRNA(Ser)CGA and other pre-tRNAs, Lhp1p is required

275 The hypomodified, but not wild-type, pre-tRNA(i)(Met) accumulates as a polyadenylated species

276 est differences in the processing of typical pre-tRNAs by the three isoforms in Arabidopsis thaliana

277 ects of los1Kan(r) cells, thereby uncoupling pre-tRNA splicing and tRNA nuclear export.

278 rocessing of the 5' and 3' ends of unspliced pre-tRNA.

279 and, less efficiently, with other unspliced pre-tRNA intermediates but not mature tRNAs.

280 strong accumulation of all tested unspliced pre-tRNA species, as well as accumulation of 5' and 3' u

281 f mature tRNAs and accumulation of unspliced pre-tRNAs.

282 Similar results were obtained using pre-tRNA(Val)s containing a 5' leader of various lengths

283 rate (k(cat)) for cleavage using the various pre-tRNA derivatives.

284 tRNase) can remove a 3' trailer from various pre-tRNAs without 5' leader nucleotides.

285 ace of mitochondria, whereas in vertebrates, pre-tRNA splicing is nuclear.

286 nism of 2'-O-methylation for the H. volcanii pre-tRNA(Trp) in vitro by assembling methylation-compete

287 rved accumulation of the excised H. volcanii pre-tRNA(Trp) intron in vivo.

288 Haloferax volcanii pre-tRNA(Trp) processing requires box C/D ribonucleoprot

289 is conserved, the subcellular location where pre-tRNA splicing occurs is not.

290 urification-tagged Lsm3p was associated with pre-tRNA primary transcripts and, less efficiently, with

291 Photocrosslinking experiments with pre-tRNA bound to RNase P reconstituted with the protein

292 activity, likely through an interaction with pre-tRNA.

293 independently, interactions of RNase P with pre-tRNA(Tyr) containing either the 5' leader, the 3' tr

294 e formation of complexes of RNase P RNA with pre-tRNA or tRNA, and at least one site stabilizes the t

295 La is the first protein to interact with pre-tRNAs in eukaryotes.

296 haromyces cerevisiae La homologue Lhp1p with pre-tRNAs was reduced approximately threefold on depleti

297 '-leader contact in the RNase P holoenzyme x pre-tRNA complex.

298 y but not for the formation of the RNase P x pre-tRNA (enzyme-substrate, ES) complex.

299 nces the magnesium affinity of the RNase P x pre-tRNA complex indirectly by binding and positioning p

300 ies of magnesium ions bound to the RNase P x pre-tRNA(Asp) complex.