ライフサイエンスコーパス: precore

1 of the pregenomic 3.5-kb RNA relative to the precore 3.5-kb RNA.

2 g)-negative than in HBeAg-positive patients (precore, 38% vs. 9%; core promoter, 51% vs. 36%; respect

3 basal core promoter T1762/A1764 mutants and precore A1896 mutants also were included, then 100% of p

4 The hepatitis B precore Ag (HBeAg) is a secreted nonparticulate version

5 clinical significance (e.g. vaccine escape, precore and antiviral-resistant mutations) and carry out

6 ime in the United Kingdom, HBeAg status, and precore and basal core promoter mutations.

7 examine the tolerogenic potential of the HBV precore and core (HBcAg) proteins, HBc/HBeAg-transgenic

8 e of e-CHB in Hong Kong and the frequency of precore and core promoter mutations in these patients.

9 ypes of hepatitis B virus were reported, and precore and core promoter mutations were found in approx

10 ians should be aware of the existence of HBV precore and core promoter variants and the clinical cond

11 In conclusion, HBV precore and core promoter variants are not rare in the U

12 era were tested for HBV genotypes as well as precore and core promoter variants by line-probe assays.

13 s in the United States and the prevalence of precore and core promoter variants in patients with ALF

14 Precore and core promoter variants were found in 27% and

15 Precore and core promoter variants were more common in h

16 equence suppresses the transcription of both precore and core RNAs, and the creation of the HNF1 bind

17 Precore and/or core promoter variants were associated wi

18 HBV precore and/or core promoter variants were common among

19 Seven of these 9 patients had precore and/or core promoter variants.

20 Sera were tested for HBV genotyping, precore, and core promoter variants by line-probe assays

21 point mutations immediately upstream of the precore AUG codon.

22 The cellular localization of the precore/core and core proteins was studied by immunofluo

23 s study, sequences of the HBV polymerase and precore/core coding regions in 26 HBV-infected patients

24 Precore/core constructs with the wild-type phenotype res

25 is derived from a translation product of the precore/core gene by two proteolytic cleavage events: re

26 ted antigen (HBcrAg), a composite antigen of precore/core gene including classical hepatitis B core p

27 cidate the impact of hepatitis B virus (HBV) precore/core gene mutations on spontaneous hepatitis B e

28 und that the woodchuck hepatitis virus (WHV) precore/core gene products (i.e., WHV core-related antig

29 e information on levels of each component of precore/core gene products as well as their biochemical

30 s study, a comprehensive characterization of precore/core gene products revealed that HBcrAg componen

31 ted antigen (HBcrAg), a mixture of the viral precore/core gene products, has emerged as one potential

32 We analyzed the HBV precore/core gene sequences by cloning method in 33 chro

33 The HBV basal core promoter and precore/core gene sequences were also evaluated in subje

34 yzed by direct sequencing of the hepatitis B precore/core gene to identify the nucleotide and amino a

35 B history, this being most pronounced in the precore/core gene.

36 HNF-4), HNF-3, and fetoprotein factor to the precore/core promoter enhancer II region.

37 e was no correlation between the presence of precore/core promoter mutations and liver disease or HBV

38 Hepatitis B virus (HBV) genotype and precore/core promoter mutations have been implicated in

39 to determine the effects of HBV genotype and precore/core promoter mutations on IFN-alpha response in

40 shown that reversion of HBV mutations in the precore/core promoter region conferring an HBeAg-negativ

41 tudy were to determine the prevalence of HBV precore/core promoter variants in the United States and

42 he presence of microsomal membranes were the precore/core protein and a truncated product representin

43 The significance of the precore/core protein being present in the nucleus is not

44 staining may be caused by expression of the precore/core protein, some of which may be translocated

45 , consequently, directs the translation of a precore/core protein, which is secreted as e antigen (HB

46 sis of the kinetics of each component of the precore/core-related antigen, along with serum viral DNA

47 A precore-deficient mutant of duck hepatitis B virus (DHBV

48 ed as e antigen (HBeAg) following removal of precore-derived signal peptide and the carboxyl terminus

49 niversally showed mutations in their genomic preCore domains impeding hepatitis B e antigen (HBeAg) p

50 d the ability of the hepatitis B virus (HBV) precore, envelope, and X gene products to modulate HBV r

51 Variants in the precore (G(1896)A) and core promoter (A(1762)T, G(1764)A

52 The reduction of precore gene expression was accompanied by an increase i

53 The function of the precore gene in the biology of hepadnaviruses is unknown

54 e experiments suggest that expression of the precore gene may be important in the regulation of HBV r

55 r, transient or stable overexpression of the precore gene resulted in striking inhibition of HBV repl

56 We found that ablation of the precore gene resulted in the generation of a hepatitis B

57 on and is not invariably associated with the precore HBV mutant.

58 ations enabled core protein translation from precore messenger RNA, which could rescue the replicatio

59 (hepatitis B e antigen [HBeAg]-positive) and precore mutant (HBeAg-negative) sequences.

60 Core protein expression from the precore mutant constructs was very much reduced, indicat

61 ining two ducklings, ratios of wild-type and precore mutant virus fluctuated, with wild-type virus sl

62 In another four ducks, the precore mutant virus slowly replaced the wild-type virus

63 ence of a rapidly replicating variant of the precore mutant, since genomes cloned from the infected d

64 cks, the wild-type virus slowly replaced the precore mutant.

65 f DHBV may be analogous to the selection for precore mutants of HBV during chronic hepatitis in human

66 either independently or in combination with precore mutation G1896A were associated with the transit

67 Reversions of precore mutations A1762T/G1764A and G1896A were observed

68 ation between HCC and basal core promoter or precore mutations in genotype F.

69 d the effect of therapy on the status of HBV precore mutations.

70 for natural HBV infection and especially for precore-negative chronic hepatitis.

71 The selection of a precore-negative strain of DHBV may be analogous to the

72 The function of the hepatitis B virus (HBV) precore or HBeAg is largely unknown because it is not re

73 that the intracellular HBeAg, also known as precore or p22, inhibits the antiviral signaling of IFN-

74 Precore (PC) (G1896A) and basal core promoter (BCP) (A17

75 We investigated whether the presence of precore (PC) and basal core promoter (BCP) mutants befor

76 conversion rates, mutational patterns in the precore (PC) and core promoter (CP) regions, severity of

77 Hepatitis B virus (HBV) precore (PC) and dual basal core promoter (BCP) mutation

78 related occurrence of flares to presence of precore (PC) and/or basal core promoter (BCP) mutants an

79 The 1896 precore (PC) mutation is the most frequent cause of hepa

80 n HepG2 cells initiated by transduction with precore (PC), rtM204I, and wild-type (wt) HBV recombinan

81 Amplified basic core promoter/precore, pre-S/S, and whole genome were sequenced, analy

82 with siRNA against the polyadenylation (PA), precore (PreC), and surface (S) regions, respectively, c

83 we were unable to detect the presence of the precore protein and e antigen from CID variants.

84 These observations suggest that the precore protein can exert a dominant negative effect on

85 ich sheds light on the enigmatic function of precore protein in shaping HBV chronicity and provides a

86 ar form of HBeAg, previously reported as the precore protein intermediate (p22) without the N-termina

87 The other produces a p25 precore protein that is targeted by a signal peptide to

88 The pre-C RNAs encode the precore protein which is proteolytically processed to yi

89 Overexpression of the precore protein, however, eliminated nucleocapsid partic

90 In summary, our study suggests that HBV precore protein, specifically the p22 form, impedes JAK-

91 slightly extended 5' end to cover the entire precore region and, consequently, directs the translatio

92 V associated with the G1896A mutation in the precore region has a high prevalence.

93 ces in the number of basal core promoter and precore region mutations between patients with HCC and c

94 he hepatitis B virus DNA sequence around the precore region was determined from sera of 45 black Sout

95 lymerase and primers targeting the conserved precore region, the HBV clones thus generated are replic

96 for possessing two nonsense mutations in the precore region, which together with the core gene encode

97 or by nonsense or frameshift mutation in the precore region.

98 for mutations in the basal core promoter and precore regions.

99 reaction (PCR) with primers in the HBV S and precore regions.

100 otein (HBc) and HBeAg and, additionally, the precore-related antigen PreC, retaining the N-terminal s

101 and e antigen (HBeAg) and, additionally, the precore-related antigen, PreC, retaining the N-terminal

102 nscription of two related RNA products named precore RNA and core RNA.

103 t HNF4 could stimulate the expression of the precore RNA and the core RNA from the core promoter of b

104 (HBV) controls the transcription of both the precore RNA and the core RNA.

105 moter that controls the transcription of the precore RNA and the core RNA.

106 factor(s) and that the transcription of only precore RNA and, consequently, the expression of e antig

107 The precore RNA codes for the secreted e antigen, while the

108 affect the WT core promoter, suppressed the precore RNA expression of the double mutant.

109 The precore RNA has a slightly extended 5' end to cover the

110 re promoter transcripts, pregenomic RNA, and precore RNA in multiple HBV cell culture models.

111 Hence, the specific suppression of precore RNA transcription by this frequent double-nucleo

112 n the core promoter, suppresses specifically precore RNA transcription, and enhances viral replicatio

113 stigated how this double mutation suppresses precore RNA transcription.

114 epatitis B e antigen (HBeAg), encoded by the precore RNA, mediates part of the inhibition of viral re

115 ifically suppresses the transcription of the precore RNA.

116 of the pregenomic RNA compared with that of precore RNA.

117 or the transcription of the core RNA and the precore RNA.

118 ORF that was truncated and fused to inverted precore sequences was found using RNAs from Hep3B cells.

119 y primer extension analysis, novel core- and precore-specific transcripts were induced by Dex which i

120 e percent of the patients with e-CHB had the precore stop codon mutation, and an additional 41% had c

121 ine-resistant mutations had reversion of the precore stop codon mutation; in 2 patients this was acco

122 ariant was detected in 16 (55%) patients and precore stop codon variant in 18 (62%) patients.

123 Precore variant was detected in 27% of patients and core

124 ID(50%), 9% chronic) and for high doses of a precore WHeAg-minus mutant WHV8 clone (17% chronic).