ライフサイエンスコーパス: prenyl

1 ither wild-type or abz1 mutant yeast to form prenyl-[(13)C(6)]pABA.

2 is of the enol ether afforded methyl 6-oxo-1-prenyl-2-cyclohexenecarboxylate.

3 The common precursor 1-methoxy-2-prenyl-3-carbomethoxycarbazole was synthesized from dime

4 a novel novobiocin analogue, in which the 3-prenyl-4-hydroxybenzoate of novobiocin is replaced with

5 ophosphate (FPP) to their protein or peptide prenyl acceptor substrates.

6 rotein to react with C2,3 double bond in the prenyl acceptor to form PSPP, with the lower two-thirds

7 Mimetics of the C-terminal tetrapeptide of prenyl acceptors have been previously shown to inhibit m

8 tool, 38 potential substrates were tested as prenyl acceptors in assays with three prenyltransferases

9 This study indicates that certain prenyl alcohol analogues can act as prenyltransferase in

10 xidizes farnesol more efficiently than other prenyl alcohol substrates.

11 The prenyl anchor of Ypt7 allows both HOPS and P-HOPS to be

12 Substitution of different prenyl anchors on Rab GTPases does not lead to correct f

13 Enzymatic prenyl and glycosyl transfer are seemingly unrelated rea

14 MTPT to protect linear peptides by attaching prenyl and methyl groups at their free N- and C-termini.

15 d monolayer lipoprotein particles containing prenyl and neutral lipids and several dozen proteins mos

16 ro, tertiary amino, and N-alkyl, N-benzyl, N-prenyl, and N-silyl tert-butyl carbamate groups were tol

17 The prenyl binding is enabled by a unique "loop-out" conform

18 d proteins is likely due to sequestration of prenyl binding protein delta (PrBPdelta) by ARL3-Q71L as

19 l organization of K-Ras is controlled by the prenyl binding protein PDEdelta, which enhances Ras diff

20 The mouse Pde6d gene encodes a ubiquitous prenyl binding protein, termed PrBP/delta, of largely un

21 pocket and renders the protein incapable of prenyl binding, is supported by molecular dynamics simul

22 We developed a prenyl-binding assay and show that RabGGTase has a preny

23 n the domain suggested to form a hydrophobic prenyl-binding pocket for the gamma subunit's prenyl gro

24 novel, unrelated chemotype that binds to the prenyl-binding pocket of PDEdelta with high affinity, th

25 t the small-molecule Deltarasin binds to the prenyl-binding pocket of PDEdelta, and impairs Ras enric

26 se (PDEdelta), which possesses a hydrophobic prenyl-binding pocket, is able to function as a potentia

27 ereas soluble PDE6 is purified with a 17-kDa prenyl-binding protein (PDEdelta) tightly bound.

28 A structure of PDE6 in complex with prenyl-binding protein (PrBP/delta) indicated the locati

29 tions with its inhibitory gamma-subunit, the prenyl-binding protein (PrBP/delta), and activated trans

30 ctor of phosphodiesterase 6 Delta (PDE6D), a prenyl-binding protein and chaperone of prenylated prote

31 pha and PDE6beta and can form a complex with prenyl-binding protein delta (PrBP/delta), an isoprenyl-

32 ments establish that PDEdelta functions as a prenyl-binding protein interacting with multiple prenyla

33 The prenyl-binding protein PDEdelta is crucial for the plasm

34 es is highly dependent on the BBSome and the prenyl-binding protein phosphodiesterase 6 subunit delta

35 lium through a motif near the C terminus and prenyl-binding protein phosphodiesterase 6D (PDE6D)-depe

36 prenylated substrate, whereas the homologous prenyl-binding protein PrBP/delta did not interact with

37 delta (delta subunit of phosphodiesterase; a prenyl-binding protein) and INPP5E (inositol polyphospha

38 Furthermore, the 17-kDa prenyl-binding protein, abundant in retinal cells, selec

39 protein prenyl transferases contain a single prenyl-binding site and suggests that RabGGTase transfer

40 finity technique for the characterization of prenyl-binding sites.

41 rolidine amide of 2-methoxybenzoic acid with prenyl bromide afforded (-)-vibralactone confirming the

42 alkylation of methyl 2-methoxybenzoate with prenyl bromide and hydrolysis of the enol ether afforded

43 h an arylcopper intermediate is reacted with prenyl bromide to afford a key intermediate that was con

44 -binding assay and show that RabGGTase has a prenyl carrier function similar to the CAAX prenyl trans

45 r prenyl pyrophosphate substrates, acting as prenyl carriers.

46 lization between amino acid nitrogen and the prenyl chain during clavicipitic acid biosynthesis can o

47 and arginine residues are non-equivalent and prenyl chain length modifies nascent polybasic domain li

48 biquinone-8 as well as quinones with shorter prenyl chains (ubiquinone-1 and ubiquinone-2).

49 ng blocks are stitched together by means of 'prenyl coupling', followed by enzymatically controlled m

50 phan fluorescence of PDEdelta and dansylated prenyl cysteines as fluorescent ligands, we show that PD

51 The prenyl-dependent binding of Narf to prelamin A is an imp

52 approach to complex terpenes whereby simple prenyl-derived chains are cyclized using radical, rather

53 of downstream mevalonate products, including prenyl-derived lipids, and prevented membrane localizati

54 Herein it is shown that certain novel prenyl diphosphate analogues are potent inhibitors of ma

55 ical reaction in all domains of life wherein prenyl diphosphate donors transfer prenyl groups onto sm

56 nes from the corresponding C10, C15, and C20 prenyl diphosphate precursors, and at the gene level by

57 rpenoid classes is the transformation of the prenyl diphosphate precursors, geranyl diphosphate, farn

58 S-e/f subfamily that utilize the unusual cis-prenyl diphosphate substrates neryl diphosphate and 2z,6

59 erestingly, LOS uses alternative C15 and C20 prenyl diphosphate substrates to produce combinatorial h

60 nylated in vitro with natural and nonnatural prenyl diphosphate substrates.

61 osphate is utilized by a membrane-associated prenyl diphosphate synthase activity to generate decapre

62 d functional characterization of a novel cis-prenyl diphosphate synthase cDNA, termed Lavandula x int

63 Rv1086 is a unique prenyl diphosphate synthase in that it adds only one iso

64 630 is the only Arabidopsis trans-long-chain prenyl diphosphate synthase that clusters with the Coq1

65 he product specificity of a trans-long-chain prenyl diphosphate synthase that elongates an allylic di

66 This is the first report of a bacterial Z-prenyl diphosphate synthase that preferentially utilizes

67 a dedicated type III polyketide synthase, a prenyl diphosphate synthase, and an aromatic prenyltrans

68 documents the presence of a short-chain cis-prenyl diphosphate synthase, previously only found in So

69 m tuberculosis H37Rv genome encodes a unique prenyl diphosphate synthase.

70 Z-prenyl diphosphate synthases catalyze the sequential con

71 The specific activities of the prenyl diphosphate synthases in M. tuberculosis are 10-

72 In Mycobacterium tuberculosis, two related Z-prenyl diphosphate synthases, E,Z-farnesyl diphosphate s

73 te is the allylic substrate for two distinct prenyl diphosphate synthases, one located in the cell me

74 Geranylgeranyl diphosphate (GGPP), a prenyl diphosphate synthesized by GGPP synthase (GGPS),

75 20-carbon prenyl group, respectively, from a prenyl diphosphate to a cysteine residue at the carboxyl

76 processive manner to generate the 50-carbon prenyl diphosphate, which is then dephosphorylated to ge

77 PS genes in the Solanum cluster encoding cis-prenyl diphosphate-utilizing enzymes are closely related

78 enzymes were found to be active on multiple prenyl-diphosphate substrates with different chain lengt

79 ral conserved sequence motifs found in other prenyl-diphosphate synthases are present in both TgFPPSs

80 use trans-prenyl diphosphates, some use cis-prenyl diphosphates and some use both.

81 ions in substrate specificity to utilize cis-prenyl diphosphates and through the acquisition of CPT g

82 TPS enzymes accept ubiquitous prenyl diphosphates as substrates and convert them into

83 that use the corresponding C10, C15, and C20 prenyl diphosphates as substrates to generate the enormo

84 te and dimethylallyl diphosphate to form C10 prenyl diphosphates that can be elongated by the additio

85 id biosynthesis is the conversion of acyclic prenyl diphosphates to terpenoid compounds by specific t

86 pene and diterpene synthases, some use trans-prenyl diphosphates, some use cis-prenyl diphosphates an

87 esis of monoterpenes and diterpenes from cis-prenyl diphosphates, substrates that are synthesized by

88 PFTase discriminates between FPP and larger prenyl diphosphates, we have examined the interactions b

89 atic substrates in the presence of different prenyl diphosphates.

90 ke all cis and trans C(10) , C(15) and C(20) prenyl diphosphates.

91 tilizes 3,3-dimethylallyl pyrophosphate as a prenyl donor and prenylates resveratrol to form arachidi

92 sis, whereas phytyl diphosphate (PDP) is the prenyl donor for tocopherol synthesis.

93 Geranylgeranyl diphosphate (GGDP) is the prenyl donor for tocotrienol synthesis, whereas phytyl d

94 nant LaPT1 for the dimethylallyl diphosphate prenyl donor is in a similar range to that of other flav

95 ino acids surrounding the allyl group of the prenyl donor.

96 el use of an iodolactone to protect both the prenyl double bond and carboxylic acid, no protecting gr

97 ariety of acyclic or cyclic monoketones with prenyl ether-tethered aldehydes in the presence of 1,8-d

98 is the covalent attachment of a hydrophobic prenyl group (either farnesyl or geranylgeranyl), which

99 , a dimer containing a gamma subunit with no prenyl group (gamma(2)-C68S) decreased binding by nearly

100 renyl-binding pocket for the gamma subunit's prenyl group (prenyl pocket mutants) and the other set i

101 The method utilizes simple chemistry on the prenyl group and cleavable properties of a sulfoxide gro

102 The CAAX isoform contains a C-terminal prenyl group and is tightly bound to endoplasmic reticul

103 eraction between the G protein gamma subunit prenyl group and PLCbeta isozymes.

104 the tail is not simply a linker between the prenyl group and the protein but that it also provides a

105 nction is independent of the identity of the prenyl group and, therefore, that FTI inhibition of K-Ra

106 both a hydrophilic cofactor and a lipophilic prenyl group attached to a polar protein substrate.

107 the penetratin vehicle and the nature of the prenyl group attached.

108 prenyltransferases (PTases) responsible for prenyl group attachment have only recently been isolated

109 structurally reminiscent of the binding of a prenyl group by a regulatory protein, the Rho guanine nu

110 The precise amino acid sequence and prenyl group define a combinatorial code for lipid bindi

111 ts support the structural model in which the prenyl group escapes contact with the aqueous milieu by

112 ase I (GGTase I) catalyzes the transfer of a prenyl group from geranylgeranyl diphosphate to the carb

113 product bearing different placements of the prenyl group in the A-ring have shown selectivity for ei

114 to the incorporation of a prenyl or reverse-prenyl group into a bicyclo[2.2.2]diazaoctane framework,

115 presumably with insertion of the C-terminal prenyl group into the membrane.

116 a-receptor interactions, suggesting that the prenyl group is an important domain in the beta gamma di

117 e that a major function of the gamma subunit prenyl group is to facilitate direct protein-protein int

118 the composition of the gamma subunit and its prenyl group markedly affects the ability of the betagam

119 Furthermore, the nature of the prenyl group on Ggamma may play an important role in the

120 The prenyl group on the G protein gamma subunit is an import

121 The role of the prenyl group on the gamma subunit in determining the act

122 o acid sequence of the gamma subunit and its prenyl group play a role in determining the activity of

123 ues that play a dominant role in determining prenyl group specificity.

124 din-2, whereas another, AhR3'DT-1, added the prenyl group to C-3' of resveratrol.

125 a phenol to the terminal olefin of a reverse prenyl group to give a dihydrobenzofuran product.

126 Furthermore, the position of the prenyl group within the phenolic skeleton also influence

127 talyze the transfer of a 15- and a 20-carbon prenyl group, respectively, from a prenyl diphosphate to

128 cPLA2gamma contains a prenyl group-binding site motif and appears to be largel

129 s through membrane insertion of a C-terminal prenyl group.

130 pt7, bound to membranes by either C-terminal prenyl groups (Ypt7-pr) or a recombinant transmembrane a

131 the hydrophobicity of the palmitate and the prenyl groups and the spacing between them.

132 Switching prenyl groups from farnesyl to geranylgeranyl or vice ve

133 translational modification with myristoyl or prenyl groups is essential for membrane association of m

134 was used to examine the effect of different prenyl groups on the gamma subunits in the activation of

135 e wherein prenyl diphosphate donors transfer prenyl groups onto small molecules as well as large prot

136 Prenylation is the addition of prenyl groups to peptide chains or metabolites via the c

137 ral basis for the successive addition of two prenyl groups to Rab proteins by the homologous enzyme g

138 bsence of statins and subsequent cleavage of prenyl groups with Raney nickel revealed that the enzyme

139 Rabs are anchored to membranes by C-terminal prenyl groups, but can also function when anchored by an

140 In contrast, prenyl groups, which are as hydrophobic as acyl chains b

141 y mevinolin, which inhibits the synthesis of prenyl groups.

142 anchored to the membrane through C-terminal prenyl groups.

143 ltered to direct modification with different prenyl groups.

144 es their post-translational modifications by prenyl groups.

145 Two "reverse prenyl" hexahydropyrroloindole alkaloids, 5-N-acetylarde

146 his study we have identified new halogenated prenyl-indole alkaloids from an invertebrate-derived Mal

147 ein-protein interactions rather than protein-prenyl interactions.

148 rfamily catalyze the attachment of prenyl or prenyl-like moieties to diverse acceptor compounds.

149 V virion assembly is critically dependent on prenyl lipid modification, or prenylation, of its nucleo

150 carotenoid formation and the channelling of prenyl lipid precursors in tomato (and its potential man

151 o the inner membrane provides access for the prenyl lipid substrate.

152 Isolated PGs from k1 k3 showed a modified prenyl-lipid composition, suggesting reduced activity of

153 C1K1/3 complex contributes to PG function in prenyl-lipid metabolism, stress response, and thylakoid

154 is reversible and mediated by the polybasic-prenyl membrane targeting motif of KRas.

155 ctive palladium(0)-catalyzed decarboxylative prenyl migration and aromatization sequence as the key s

156 Protein prenylation, the specific prenyl modification (farnesyl or geranylgeranyl), as wel

157 alter the position of the post-translational prenyl modification at the C terminus of the gamma subun

158 To determine the role of the prenyl modification in the interaction of beta gamma dim

159 Prenyl modification of the gamma subunit is necessary fo

160 s in living cells revealed that acyl but not prenyl modifications promote clustering in lipid rafts.

161 ions of AIPL1-FK506-binding protein with the prenyl moieties of PDE6 and AIPL1-TPR with the Pgamma su

162 isoprenoid compounds involve transfer of the prenyl moiety in allylic diphosphates to electron-rich (

163 synthetic pathways, we demonstrated that the prenyl moiety on the prenylated stilbenoids derives from

164 ion energy, indicating direct binding of the prenyl moiety to PLCbeta.

165 d menaquinones, both containing an essential prenyl moiety, are key electron carriers in respiratory

166 We conclude that the polybasic-prenyl motif acts as a Ca2+/CaM-regulated molecular swit

167 ccurs through sequestration of the polybasic-prenyl motif by Ca2+/calmodulin (Ca2+/CaM) and subsequen

168 using prenylated peptides which mimicked the prenyl motifs in the proteins.

169 P-MurNAc-pentapeptide (Park's nucleotide) to prenyl-MurNAc-pentapeptide (lipid I), the first membrane

170 dented 3,4-dihydro-1-benzazepine-2,5-dione-N-prenyl-N-acetoxy-anthranilamide scaffold, was isolated f

171 MATS) superfamily catalyze the attachment of prenyl or prenyl-like moieties to diverse acceptor compo

172 equences that lead to the incorporation of a prenyl or reverse-prenyl group into a bicyclo[2.2.2]diaz

173 f oxindoles to afford selectively either the prenyl or reverse-prenyl product has been demonstrated.

174 ctivated Ypt7, whether bound to membranes by prenyl or transmembrane anchor.

175 lse-labeling analyses show that formation of prenyl-pABA occurs within minutes and precedes the synth

176 A hexaprenylated form of pABA (prenyl-pABA) is normally present in wild-type yeast crud

177 activity was also exquisitely sensitive to a prenyl peptide analogue that had been previously describ

178 We recently identified a prenyl peptide-binding protein in microsomal membranes f

179 inability of a 15-C FPP to displace the 20-C prenyl-peptide product.

180 mino group; N-acetylated prenylcysteines and prenyl peptides are not substrates.

181 Prenyl peptides bind to purified tubulin with a Kd of 40

182 of the minimum structure requirement of the prenyl phosphate in the MraY/MurX-catalyzed lipid I anal

183 is the first bacterium reported to utilize a prenyl phosphate other than undecaprenyl phosphate as th

184 f IL-15, a T cell growth factor, to modulate prenyl phosphate-induced gamma delta T cell proliferatio

185 particular a class of compounds known as the prenyl phosphates.

186 nge (beta), and residues lining the proposed prenyl pocket (beta).

187 n order to accurately predict the effects of prenyl pocket amino acid substitutions.

188 ith the aqueous milieu by inserting into the prenyl pocket and stabilizing the Pdc-binding conformati

189 substitution of certain residues within the prenyl pocket caused only minor decreases in binding, wh

190 sertion of the geranylgeranyl group into the prenyl pocket in order to accurately predict the effects

191 The prenyl pocket mutants expressed with gamma(2) were 10-fo

192 pocket for the gamma subunit's prenyl group (prenyl pocket mutants) and the other set in a domain bet

193 ord selectively either the prenyl or reverse-prenyl product has been demonstrated.

194 ously described as a potent inhibitor of the prenyl protease activity in mammalian tissues.

195 infected Sf9 cells exhibited a high level of prenyl protease activity.

196 domains of life, including the type II CAAX prenyl proteases and their prokaryotic homologs with put

197 tography of extracts from cells in which the prenyl protein pool was metabolically labeled revealed t

198 The additional modifications in the prenyl protein processing pathway also affected the inte

199 A putative prenyl protein protease in yeast, designated Rce1p, was

200 d by its sensitivity to RPI, an inhibitor of prenyl protein protease.

201 d the human RCE1 gene products are bona fide prenyl protein proteases and suggest that they play a ma

202 rt due to the ability of tubulin to bind the prenyl protein reaction product.

203 This enzymatic activity, termed prenyl protein-specific endoprotease (PPEP) activity, ha

204 reviously been shown to be recognized by any prenyl:protein transferase (PTase), but which most close

205 pool was metabolically labeled revealed that prenyl proteins bound to the immobilized microtubules; o

206 lying the presence of a pool of unmethylated prenyl proteins in these cells under normal conditions.

207 ing evidence that C-terminal modification of prenyl proteins, rather than being purely a constitutive

208 key photochemical valence isomerization of 3-prenyl-pyran-2-one produces both the all-carbon quaterna

209 -nonreactive TCR abrogated reactivity to the prenyl pyrophosphate Ag isopentenyl pyrophosphate and to

210 1 chain from an Ag-nonreactive TCR yielded a prenyl pyrophosphate Ag-nonreactive TCR.

211 Human gamma delta T cells recognize prenyl pyrophosphate Ags and their analogues in a V gamm

212 uctural requirements for recognition of such prenyl pyrophosphate Ags by gamma delta T cells.

213 , we show that gamma delta TCR reactivity to prenyl pyrophosphate Ags is dependent upon the junctiona

214 to achieve stable association of nonpeptide prenyl pyrophosphate Ags with the presenting molecule.

215 acterial supernatant containing a mixture of prenyl pyrophosphate Ags.

216 junctional region sequences on reactivity to prenyl pyrophosphate Ags.

217 elta2 antigen receptors recognize nonpeptide prenyl pyrophosphate and alkylamine antigens.

218 was observed in response to stimulation with prenyl pyrophosphate antigens (isopentenyl pyrophosphate

219 T cells are stimulated by self- and foreign prenyl pyrophosphate intermediates in isoprenoid synthes

220 sponses to microbial pathogens by monitoring prenyl pyrophosphate isoprenoid metabolites.

221 These results show that prenyl pyrophosphate recognition is primarily by germlin

222 BTN3A1 proteins for their ability to mediate prenyl pyrophosphate stimulation of Vgamma2Vdelta2 T cel

223 philin (BTN)3A1 was shown to be required for prenyl pyrophosphate stimulation.

224 the enzymes form stable complexes with their prenyl pyrophosphate substrates, acting as prenyl carrie

225 delta T cells are stimulated when exposed to prenyl pyrophosphate, bisphosphonate, and alkylamine Ags

226 Thus, recognition of nonpeptide prenyl pyrophosphate, bisphosphonate, and alkylamine Ags

227 Although a prenyl pyrophosphate-binding site was defined by Lys109

228 ding V regions of the B30.2 domain abrogated prenyl pyrophosphate-induced proliferation.

229 did not preferentially bind a photoaffinity prenyl pyrophosphate.

230 of HMBPP, meta/para-benzophenone-(methylene)-prenyl pyrophosphates (m/p-BZ-(C)-C(5)-OPP), can crossli

231 Longer prenyl pyrophosphates also retained bioactivity.

232 amma2Vdelta2(+) T cells recognize nonpeptide prenyl pyrophosphates and alkylamines.

233 Several findings suggest that prenyl pyrophosphates are presented by an Ag-presenting

234 Thus, BTN3A1 is required for stimulation by prenyl pyrophosphates but does not bind the intermediate

235 se findings support intracellular sensing of prenyl pyrophosphates by BTN3A1 rather than extracellula

236 features that determine the antigenicity of prenyl pyrophosphates by testing synthetic analogs for b

237 quirements of TCR gamma delta recognition of prenyl pyrophosphates distinguish this reactivity from t

238 at the extracellular BTN3A1 IgV domain binds prenyl pyrophosphates, leading to the proposal that the

239 e intracellular B30.2 domain of BTN3A1 binds prenyl pyrophosphates, resulting in a change in the extr

240 ing Vgamma2Vdelta2 TCRs recognize nonpeptide prenyl pyrophosphates.

241 e existence of an Ag-presenting molecule for prenyl pyrophosphates.

242 g infections by recognizing small nonpeptide prenyl pyrophosphates.

243 (BTN3)/CD277 Ig superfamily proteins mimics prenyl pyrophosphates.

244 ive to statin inhibition than stimulation by prenyl pyrophosphates; however, the continuous presence

245 Prenyl Rab-GDI complexes contain all of the information

246 The late endosomal, prenyl Rab9 binds GDI with very high affinity, which led

247 of 4-hydroxy-3-prenylbenzoate with different prenyl side chain lengths.

248 redox active benzoquinone ring attached to a prenyl side chain.

249 n of the 7-hydroxyl group and removal of the prenyl side chain.

250 sion of prenylated proteins as measured with prenyl-specific antibody, and inhibits parasite differen

251 A prenyl substituent was incorporated at the C3 position o

252 The prenyl-substituted aryl ester of 3,5-dihydroxychroman-3-

253 yme could utilize solanesyl diphosphate, the prenyl substrate for plastoquinone-9 synthesis.

254 rmed by converting farnesyltransferase (15-C prenyl substrate) into GGTase-I (20-C prenyl substrate)

255 (15-C prenyl substrate) into GGTase-I (20-C prenyl substrate) with a single point mutation.

256 f the chiral catalyst and the stereoisomeric prenyl substrate, full control of the stereo- and enanti

257 lgeranyl transferase inhibitor GGTI-298, and prenyl substrates (farnesyl pyrophosphate [FPP] and gera

258 The biological role of prenyl substrates has made these reactions significant t

259 ture is similar to that of the bacterial cis-prenyl synthase, undecaprenyl diphosphate synthase (UPPS

260 nd structure-based mechanism of this unusual prenyl synthase.

261 Plasmodium falciparum using a library of 560 prenyl-synthase inhibitors.

262 etergent micelles, whereas that with a short prenyl tail (CoQ(1)) remains in solution.

263 simultaneously to the plasma membrane via a prenyl tail and to the E2 in planta.

264 Alternatively, enzyme catalyzed prenyl transfer provides excellent regio- and stereo-spe

265 .FPP.protein ternary complex for the ensuing prenyl transfer reaction.

266 tudies led us to propose a mechanism for the prenyl transfer reaction.

267 the five-carbon isoprene unit for subsequent prenyl transfer reactions.

268 reaction is limited by a step subsequent to prenyl transfer such as release of products from the enz

269 at are synthesized by enzymes encoded by cis-prenyl transferase (CPT) genes also located within the s

270 A short-chain cis-prenyl transferase (LfCPT1) first produces the rare dite

271 roblems in cellular isoprenoid metabolism or prenyl transferase activity were investigated, but none

272 The protein adopts the zeta or cis-prenyl transferase fold but remarkably, unlike tuberculo

273 Ad, and its crystal structure revealed a cis-prenyl transferase fold with hydrophobic residues for is

274 e critical structural properties determining prenyl transferase specificity and in metabolic engineer

275 e sensitivity to existing drugs, and the cis-prenyl transferase undecaprenyl diphosphate synthase (UP

276 prenylation of K-Ras by the closely related prenyl transferase, geranyl geranyl transferase I, and t

277 ns of the protein structure indicated that a prenyl-transferase domain and several transmembrane heli

278 ettled wisdom with the discovery of a fourth prenyl-transferase, namely geranylgeranyl-transferase-II

279 erculosinol adenosine synthase and other cis-prenyl transferases (e.g. cis-farnesyl, decaprenyl, unde

280 as to the mechanism of action of many other prenyl transferases and may also be of use in engineerin

281 mechanism and inhibition of the head-to-head prenyl transferases and should aid future drug design.

282 s generated considerable interest in protein prenyl transferases as possible anticancer targets.

283 his study establishes that all three protein prenyl transferases contain a single prenyl-binding site

284 progress has been made in understanding how prenyl transferases distinguish between related target p

285 Previous characterization of CAAX prenyl transferases showed that the enzymes form stable

286 om C(5) precursors; the epsilon head-to-head prenyl transferases that convert these diphosphates into

287 terpene synthases; the zeta head-to-tail cis-prenyl transferases that produce the cis-isoprenoid diph

288 alphaalpha and alphadelta head-to-tail trans-prenyl transferases that produce trans-isoprenoid diphos

289 Small molecule inhibitors of prenyl transferases, enzymes that catalyze the post-tran

290 Through the addition of prenyl transferases, farnesyl diphosphates, (2E,6E)-FDP

291 Three distinct protein prenyl transferases, one protein farnesyl transferase (F

292 ket distinguishes Rv3378c from classical cis-prenyl transferases, providing a unique model for the pr

293 prenyl carrier function similar to the CAAX prenyl transferases.

294 (farnesyl or geranylgeranyl), as well as the prenyl-transferases involved can be inferred by protein

295 s motifs direct modification by one of three prenyl-transferases, has been an area of fairly settled

296 l-CoA, carbamoyl-P), methyl transfers (SAM), prenyl transfers (IPP), glucosyl transfers (UDP-glucose)

297 This rearrangement may be conducted with prenyl-type selenosulfides to give isoprenyl alkyl sulfi

298 Lipid I isolated from Mtb contains the C50-prenyl unit that shows very poor water solubility; thus,

299 a noncanonical route to the construction of prenyl units and serves as a prototype for the intersect

300 hates, including those bearing repeating cis-prenyl units.