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1 ing to define a sensitive ground truth of HP presence.
2 ment in areas of late gadolinium enhancement presence.
3  factors that gauge this compactness are the presence/absence of a radial-node in the valence-orbital
4                                              Presence/absence of AD-like pathology was donor-specific
5         We studied B cell development in the presence/absence of autoantigen by crossing the Ig KI mi
6  and in their conformational dynamics in the presence and absence of Ca(2+) Only AtSCS-A has the feat
7 ere detected by NMR spectroscopy both in the presence and absence of Cu at a common interface.
8 d rat liver mitochondrial respiration in the presence and absence of saturating solutions of CO (160
9 sh Danio rerio and testing the effect of the presence and absence of the germ line on somatic repair
10 ospectively studied associations between LGE presence and adverse cardiovascular events in patients w
11 y component of the disease, particularly the presence and evolution of focal lesions - the pathologic
12  aggregation pheromone that signals nestmate presence and induces arrest in the termite Reticuliterme
13 omising noninvasive approach to identify the presence and potentially monitor the persistence and pro
14 ent preoperative evaluation is to assess the presence and severity of small-vessel arteriopathy.
15                                          The presence and severity of various characteristics of drus
16 ter, settled aerosol, or harvested air), the presence and type of at-source aerosol reduction methods
17 o its frequency over years and indicated its presence in 94.1 and 93.7% of lines in the 2019 IBWSN an
18 ssible clinical utility, many of which whose presence in blood is likely linked to specific cell-leve
19 investigate microaspiration and pneumococcal presence in the lower airways.
20  prior to treatment in most patients and its presence is strongly prognostic.
21 asicrystalline and approximant phases, whose presence is tunable by layer number.
22 ey may be recruited to tumors, and how their presence may give rise to aggressive disease.
23                                              Presence of >=1 of these features should be considered a
24                                       In the presence of 0.25 M exogenous sucrose, increased cataboli
25 rene dicarboxylic acid self-assembles in the presence of 1,12-diaminododecane to yield overall neutra
26                                          The presence of 1,2-unsaturated PA in foods is a concern to
27              No interference was observed in presence of 100 ppb arsenic, 20 ppb copper, 5 ppb cadmiu
28                                       In the presence of 2 factors or more upfront transplantation sh
29 with 2.3 equivalents of KC(8) in THF, in the presence of 2.2.2-cryptand, at -78 degrees C.
30 rthermore, the pathway analysis revealed the presence of 229 and 943 genes involved in the phenylprop
31 ere evaluated in a randomized manner for the presence of 3 tomographic features of edema.
32 yotes is not restricted to animals or to the presence of 4D-Na(v)s.
33  a wide pH range (pH 1-14), including in the presence of 5 M NaOH.
34                                 However, the presence of 50 g/L lactose reduced the RFP intensity due
35 his work, (71) Ga NMR spectroscopy shows the presence of [Ga(arene)(n) ](+) salts on oxidation of Ga
36    Many of these reduction steps require the presence of a catalyst to activate the reducing agent.
37                                       In the presence of a chiral catalyst, both the central and axia
38                                              Presence of a common disease haplotype among this cohort
39 reduction in electrical conductivity-and the presence of a complex network of passive components that
40 xamined included aneurysm wall irregularity, presence of a daughter dome, presence of hypoplastic or
41 0N-NS1 RBD interaction in vitro requires the presence of a dsRNA platform that binds both NS1 RBD and
42 and filtering are only possible owing to the presence of a gasket-like structure (forming a canopy ab
43 oop was evolved by infectious cycling in the presence of a helper adenovirus to yield a new AAV varia
44 he constant thickness (11 km), requiring the presence of a mantle thermal anomaly extending up to 2.6
45 M deficient fibroblasts and may indicate the presence of a mechanism to protect 3D genome structure i
46 e dimension this transition is stable in the presence of a metallic Fermi sea, and its universality c
47   A true-positive finding was defined as the presence of a pathology-confirmed cancer within 12 month
48 minals is likely to increase, indicating the presence of a previously unknown degree of functional co
49 lcohol can be protected in good yield in the presence of a primary ammonium salt or a carboxylic acid
50 ase/dNTP replication machinery leads, in the presence of a primer, P(1), to the gated replication of
51 ssociated functions of RC also depend on the presence of a specific set of host proteins.
52 PROs as a primary endpoint (p=0.008) and the presence of a subsequent publication on PROs (p<0.0001).
53 ide natural products that are unified by the presence of a substituted, nitrogen-containing heterocyc
54 hermolytic decomposition of Mo(CO)(6) in the presence of a surface-stabilizing ligand and a high boil
55      Further FT-IR spectroscopy revealed the presence of a W=O moiety arising from the in situ reacti
56 ment of active-site amino acids revealed the presence of a water-coordinating aspartate residue that
57 eed germination and seedling greening in the presence of ABA, whereas knockout of ABT has the opposit
58     Absence of a conserved basic residue and presence of acidic residues in the vestibule regions, co
59                 We also demonstrate that the presence of acoustic communication is strongly conserved
60  ClpP1 and ClpP2 rings and also requires the presence of activator peptides, such as benzoyl-leucyl-l
61  between symptoms of gastroenteritis and the presence of airborne norovirus, and to investigate the s
62 ch we independently varied daylength and the presence of ALAN.
63 cloisomerization that not only tolerates the presence of all of the oxygen functionalities, but also
64                                       In the presence of amines, the degradation of glutaconaldehyde
65                          For example, in the presence of ammonium acetate, at low flow rates, lower c
66 is process may be greatly potentiated by the presence of amphipathic components of plasma membrane be
67 e associated in AD-vulnerable regions in the presence of amyloid positivity in the AD clinical spectr
68  Alzheimer's disease is characterized by the presence of amyloid-beta and tau deposition in the brain
69 re at high risk of disease as defined by the presence of an adverse-risk karyotype, the presence of s
70 , consistent with its low expression and the presence of an alternative pathway for Ca(2+) uptake int
71 he ED peptide enhanced neuritogenesis in the presence of an androgen receptor inhibitor to the extent
72 nown pre-existing systemic malignancy or the presence of an atypical orbital mass in addition to enla
73  mobility was negatively associated with the presence of an endotracheal tube (adjusted odds ratio, 0
74 perboloid with an elliptical wormhole in the presence of an in-plane electric field.
75 e, and of the device selectivity through the presence of an internal reference electrode.
76                           Experiments in the presence of an osmoticant agent suggest that channel gat
77                                       In the presence of any high-risk pathologic features (nodal or
78 proportion of Tregs, the side effects in the presence of ART prevent their clinical use and call for
79                               Similarly, the presence of artifacts on the RNFL scan was significantly
80 tory illnesses, and we correlated these with presence of asthma at 6, 8, 11, 13, and 18 years of age.
81 80 angstrom resolution structure reveals the presence of ATP and GMP at the canonical sites of the Ba
82 mulate CheA kinase activity depending on the presence of attractants or repellants, respectively.
83 rder (ASD) and their families, including the presence of autoantibodies reactive to fetal brain prote
84 ring I from paromomycin that proceeds in the presence of azides and affords a glycosyl acceptor for t
85 of secondary progressive MS at 30 years were presence of baseline infratentorial lesions and deep whi
86 on and sequential B-Mes bond cleavage in the presence of BBr(3) .
87                            Nevertheless, the presence of BDG can still be recognised by the host's in
88 largely disordered species and suggested the presence of beta-sheets.
89                                       In the presence of BFT, pharmacological inhibition of GCS cause
90 ratory distress syndrome did not require the presence of bilateral infiltrates.
91  the Ni(II) -> N(IV) protocol, either in the presence of bipyridine, or using the excess of the >P(O)
92                                       In the presence of both hematite and Fe(2+)((aq)), NTO was quan
93 PCR, or droplet digital PCR to determine the presence of BRAF(V600), NRAS(Q61), TERT(-124C>T), and TE
94  poly-ADP ribosylation (PARsylation) and the presence of BRCA1-bound RAP80 are critical for the norma
95 g energetics of Syt1 both in the absence and presence of Ca(2+).
96 curately described the data collected in the presence of carbonate when we accounted for the effect i
97  respectively, at the indole scaffold in the presence of catalytic iodine and air at 40 degrees C in
98 complex multidomain proteins, folding in the presence of chaperones, and the ability to investigate r
99 ng different mechanisms, but all require the presence of cholesterol to pierce lipid bilayers.
100                                          The presence of chronic PAMM lesions was evaluated for both
101 Inpp5e(rdg) function largely depended on the presence of cilia and on smoothened, the obligate transd
102  Clinic Liver Cancer (BCLC) B and C, and the presence of cirrhosis, respectively.
103 lower amount of KChIP3 bound to Kv4.2 in the presence of CLN3.
104 o tested negative for COVID-19 regarding the presence of clots and presenting symptoms.
105 lication stress and DNA damage depend on the presence of co-transcriptional RNA/DNA hybrids (R-loops)
106 ge, 10-27 y) with CDCS were assessed for the presence of cognitive and behavioral symptoms using a ba
107 pret for a positive event, especially in the presence of competing risks.
108 nt bone marrow cells, both in the absence or presence of competition.
109 adiosilver isotopes is often thwarted by the presence of concomitant and dominating gamma emitters, p
110 m of this pilot study was to investigate the presence of concurrent donor-specific memory B cell-deri
111                          The ever-increasing presence of contaminants in environmental waters is an a
112 s of antibiotics that are only active in the presence of copper ions, are effective bactericidal agen
113            It concludes that even though the presence of cotton wool appearance in retina is a nonspe
114                                       In the presence of CRISPR-Cas immunity, full-length AcrIIA1 use
115         Recent reports indicate that, in the presence of crowding agents, tau can undergo liquid-liqu
116                                 However, the presence of cryptic lineages within the upwelling zone c
117 ncreased Cu load and decreased growth in the presence of Cu, which was abrogated by the introduction
118  95% CI 1.50-22.54) remained associated with presence of cysts and solid lesions in HRIs.
119               Our findings indicate that the presence of cytosolic DNA and subsequent activation of c
120 ssentiality of darG(Mtb) is dependent on the presence of darT(Mtb) , but simultaneous deletion of bot
121                                          The presence of difficult bag-mask ventilation is independen
122 ellular hemoglobin solutions, indicating the presence of diffusional barriers in intact cells.
123                  Colonic diverticulosis (the presence of diverticula in the colon; referred to as div
124 sulin were not significantly affected in the presence of DMNDC, suggesting that DMNDC does not inhibi
125                                          The presence of DNA damage increases the frequency of pausin
126  photodynamic therapy (PDT) treatment in the presence of DPP, resulting in attenuated cancer cell gro
127 PTPN14 and CAV1 co-inmunoprecipitated in the presence of E-cadherin in B16F10 melanoma and other canc
128 ver, in this "silent coupling" scenario, the presence of effector causes a change in the DeltaH assoc
129 d 3%) were incorporated in the recipe in the presence of either 10% sourdough or chemically acidified
130 ion occurs below 0 degrees C, reflecting the presence of elevated amounts of unsaturated fatty acid c
131                      Sugar alone, and in the presence of emulsifier, hindered the polymorphic form IV
132 pts was eventually resumed, showing that the presence of enhancer sequences, rather than either their
133  bi-allelic variants in UBR1, notably by the presence of epilepsy and the absence of exocrine pancrea
134                                          The presence of expansin genes in diverse species of bacteri
135 ts and halogenated purine nucleosides in the presence of Fe(acac)(3)/CuI is described.
136 ained cases of NIHF that were defined by the presence of fetal ascites, pleural or pericardial effusi
137                             Judging from the presence of fine versus beaded terminals, the vast major
138  for central macular thickness (CMT) and the presence of fluid over the 10 years.
139  days of gene transfer, correlating with the presence of functional effector and regulatory T-cell su
140            We aimed to ascertain whether the presence of fungi, in particular Aspergillus fumigatus,
141 fluorescence staining and immunoblotting the presence of galectin-8 within the mitotic apparatus.
142                                    While the presence of garlic in the mixture can explain the activi
143  sputum production poorly correlate with the presence of GCH or COPD.
144 ion species in two spatial dimensions in the presence of general quartic contact interactions.
145 zer as it is converted to a PS by the mutual presence of glutathione (GSH) and hydrogen peroxide (H(2
146 en cyclic and acyclic forms, alone or in the presence of glycine under moderate temperature drying co
147                                          The presence of gold as nanoparticles in the solutions of th
148  mitochondrial function is maintained in the presence of H(2)S in sulfide spring P. mexicana but not
149                         Despite the abundant presence of HDAC inhibitors such as butyrate in the inte
150                                       In the presence of heparin, the monovalent Fab shows essentiall
151 principles: 1) open the lung to minimize the presence of heterogeneity-induced stress concentrators t
152 be tomography of these crystals revealed the presence of heterogeneous spatial sequences of metal ion
153 tro antibacterial activity was stable in the presence of high bacterial inoculum size compared to van
154  sequence comparison were used to detect the presence of HIV superinfection.
155 odds of POD1 IOP spike >40 mmHg, whereas the presence of HMS was associated with a lower likelihood o
156  dSTACs elicit ER subtype-selectivity in the presence of hormone, most synergize with 17beta-oestradi
157 e to recruit ribosomal machinery only in the presence of host factor PCBP2 that binds to stem-loop IV
158                Population studies reveal the presence of HPG and KoRV-related sequences in several lo
159 l irregularity, presence of a daughter dome, presence of hypoplastic or aplastic A1 arteries and hypo
160            A hallmark of MS diagnosis is the presence of IgG Abs, which appear as oligoclonal bands i
161                            We have shown the presence of increased type 2 inflammation in patients wi
162 e hormone secreting cells; and (ii) elevated presence of innate immune cells.
163 s the knee joints, which we attribute to the presence of intact cutaneous mechanoreceptors.
164 erized by dopaminergic neuronal loss and the presence of intra-neuronal Lewy body (LB) inclusions wit
165 80-fold increase in hepcidin affinity in the presence of iron.
166 ate changes in depolymerization based on the presence of islands of uncompressed (GMPCPP) dimers in t
167 AM16L affected seed germination, even in the presence of its seemingly identical paralog, AtPAM16.
168 Circular dichroism spectroscopy revealed the presence of largely disordered species and suggested the
169 nce of CNV activity on FFA identified by the presence of leakage, and (3) cross-tabulation of CNV act
170  antioxidants was remarkably promoted in the presence of lecithin (L/GA/MG > L/GA > L/MG).
171 EV yield from human serum, together with the presence of lipoprotein and protein contaminants.
172                    These results support the presence of localized anoxic microsites in the otherwise
173 volving atmospheric pressure conditions, the presence of many different chemicals, and an electrical
174 he metabolic fingerprinting and revealed the presence of metabolites with significant influence on th
175              The definitive evidence for the presence of metaphosphates, reported here in whole bacte
176                                          The presence of methane and other hydrocarbons in domestic-u
177 ive site, however, in different modes in the presence of Mg(2+) versus Mn(2+) ions.
178 ne (8-oxodG) are efficiently ligated and the presence of Mn(2+) stimulates this coupled reaction in v
179                                       In the presence of Mn, the mechanism of As removal varied with
180 er into TPP molecules occurs at <+1 V in the presence of mobile ions, enabled by ionic screening of t
181                                          The presence of monovalent cations resulted in aggregation a
182 aigns, the environmental suitability for the presence of mosquitoes and viruses becomes one of the mo
183 s review we summarize recent findings on the presence of movement-related signals in sensory areas an
184                           When combining the presence of MRI findings, facial morphological variants,
185  UK Biobank, we found that regardless of the presence of multimorbidity, engaging in a healthier life
186                        ESI-IM-MS reveals the presence of multiple conformers for the dimer, tetramer,
187 e regions may be especially sensitive to the presence of multiple objects.
188                                          The presence of multiple pathways is rationalized by the str
189 etry was observed in assays performed in the presence of Munc18-1, Munc13-1, NSF and alphaSNAP, which
190 onomer 4 and P for Al(3+) was also proved in presence of Na(2)EDTA by both UV-Vis and fluorometric ti
191 led mutant virions in mammalian cells in the presence of NAbs.
192 opic components of BSA/lutein binding in the presence of NaSCN decreased as the temperature increased
193  ideal for clinical xenotransplantation, the presence of naturally-existing antibodies to TKO pig cel
194 igh negative fixed charge density due to the presence of negatively charged aggrecan glycosaminoglyca
195 ren; however, there was no difference in the presence of neutralizing antibodies.
196 (O)H reagents with PhX (X = I and Br) in the presence of NiCl(2)/Zn as the precursors for the assumed
197                     We recently reported the presence of nicotinamide adenine dinucleotide (NAD)-capp
198 is activity has often been attributed to the presence of nitrogen-coordinated, single Ni atom active
199 CR data that has been largely ignored is the presence of non-detects: reactions failing to exceed the
200 mps and HOMA of insulin resistance), and the presence of nonalcoholic fatty liver disease (NAFLD) and
201 lying these symptoms can be explained by the presence of nonfiring pacemaker cells.
202  military facility to confirm the absence or presence of nuclear weapons and fissile materials.
203                                       In the presence of O(2) , Aer2 signaling increases the autophos
204             In silico analysis predicted the presence of only two short allergenic peptides on the su
205 d trophic diversification depend more on the presence of optimal resources than on ecological opportu
206 e in flux in MG1655:: tetA when grown in the presence of OTC compared to growth without OTC.
207 alization by [B(12)X(11)](-) occurred in the presence of other more reactive functional groups.
208  protocol for trace silver extraction in the presence of overwhelming activities of (137)Cs via the a
209  in pig kidney, ATP was rapidly generated in presence of oxygen (100 kPa), which remained stable up t
210  infrared (ATR-FTIR) spectroscopy revealed a presence of oxygen-containing functional groups on the D
211 id formation of diformazan in the absence or presence of paraformaldehyde, (4) addition of S-nitrosot
212 ved O-PTIR and AFM-IR spectra confirming the presence of peaks related to fluticasone propionate at 1
213 ired from individual liposomes confirmed the presence of peaks related to N-H bending vibration, C-N
214 rried out ITC demicellization studies in the presence of PEG3350, finding in all cases that PEG had s
215 r and that the binding is facilitated by the presence of phosphatidylserine.
216 FIXa responds to assembly with FVIIIa in the presence of phospholipids.
217 o support histone pre-mRNA processing in the presence of polyadenylation factors from nuclear extract
218  risk scores across populations suggests the presence of population-specific components of genetic ar
219                  This study investigates the presence of potentially oncogenic viruses such as SV40,
220 sing nonperfusion, worsening DR severity and presence of PPL.
221 le composition and the sensory effect of the presence of Quaker beans in natural specialty coffee bev
222                                       In the presence of rapid cultural, environmental, or genetic ch
223         Last, simulations indicated that the presence of RBD near the membrane led to a local enrichm
224                        DNAN reduction in the presence of RDX was evaluated in abiotic experiments usi
225 yrosine adducts of alphaSyn that form in the presence of reactive oxygen species and cytochrome c, a
226 e cell wall-membrane interface, implying the presence of reducing-end glycans at this location where
227 treatments at-or close to-the source, in the presence of residual nonaqueous liquid (NAPL).
228          Compared with complete closure, the presence of residual shunt after PFO closure was associa
229 maging studies are necessary to rule out the presence of retained foreign bodies.
230 0.001), and among asymptomatic controls with presence of rhinovirus infection (OR:4.23; 95%CI 1.16-14
231 ultiple mutations that are beneficial in the presence of rifampicin and deleterious in its absence.
232     Here, we report on acoustically detected presence of right whales in MB over a nearly 6 year peri
233 tuated by retinoic acid treatment and by the presence of risk alleles at the Pitx2 locus.
234                 Images were assessed for the presence of RMA.
235                                       In the presence of RyRp at high Ca(2+), the closed conformation
236                              We document the presence of SARS-CoV-2 RNA associated with platelets of
237 the majority becoming less accessible in the presence of saturating concentrations of both Na(+) and
238 e presence of an adverse-risk karyotype, the presence of secondary acute myeloid leukaemia arising fr
239                                       In the presence of serum albumin, the potency of JMS-053 as an
240 cies and the in-depth analysis uncovered the presence of several antimicrobial resistance genes.
241 ipids like cardiolipins and demonstrated the presence of several ubiquinones in Arabidopsis mitochond
242 ions are selected during viral growth in the presence of single antibodies.
243 lasma expanding from a thermal source in the presence of spatially diverging magnetic-field lines.
244           It has been also observed that the presence of specific glucuronidase could yield free syst
245 esis of clinically-relevant molecules in the presence of specific IgG biomarker antibodies.
246              However, acidity levels and the presence of specific volatiles (such as acetate esters a
247                        However, the combined presence of spin-orbit coupling and magnetic field break
248  vivo tracking experiments have revealed the presence of SSCs not only within the bone marrow but als
249 ges were evaluated by three radiologists for presence of stenosis or occlusion.
250 n be confounded by genomic heterogeneity and presence of sub-clonal mutations.
251 olids for decades, our study establishes the presence of sub-THz frequency tunneling systems even in
252  for (1) evidence of CNV activity on SD OCT (presence of subretinal fluid, intraretinal fluid, and/or
253 ra, should be approached with caution in the presence of substrate heterogeneity or variable sample t
254  to all positions tested is modulated by the presence of substrates, with the majority becoming less
255 coordinated regional policies adopted in the presence of such spillovers.
256                                       In the presence of sufficient NaCl or osmolytes, trehalose and
257                                          The presence of sulfide can lead to the formation of thiolat
258 f the quasicrystal, Ta(21)Te(13), reveal the presence of symmetry-protected nodal crossings in the qu
259                                       In the presence of target cell receptors, an immunocomplex was
260                                              Presence of teratoma in ORCH and PC-RPLND specimens was
261                   The synergy depends on the presence of the active promoter/origin.
262           We could not find evidence for the presence of the CA2 and CA4 fields of the cornu Ammonis
263 tion across Symbiodiniaceae species, and the presence of the canonical CDK motif, CDKB emerged as a l
264                             Infection in the presence of the capsid destabilising small molecule PF-7
265                                       In the presence of the dimeric protein, the network yields a co
266 and allow cancer cells to proliferate in the presence of the HER2 kinase inhibitor lapatinib.
267 d of greatest concern in this respect is the presence of the historical, but still recycled, flame-re
268 mic mastocytosis (advSM) is characterized by presence of the KIT D816V mutation and pathologic accumu
269 ilar energetics of binding regardless of the presence of the leader peptide, suggesting that although
270                                       In the presence of the self-assembled capsule, a kinetic and th
271 hogonality of the leveraged modes and to the presence of the spherical constant-e(r) lens which incre
272 ported for directly confirming the localized presence of the template.
273 syndrome or myeloproliferative neoplasm, the presence of therapy-related acute myeloid leukaemia, or
274            To obtain direct evidence for the presence of these uracils, we engineered a protein that
275 were frozen melt sills, they would imply the presence of thick young oceanic lithosphere (20-25 km),
276 xidation reactions, in either the absence or presence of thiourea as a cocatalyst, was obtained.
277 ogen, and several lines of evidence link the presence of this bacterium in the circulation with vascu
278         Testing was performed to confirm the presence of tick-borne pathogens by real-time PCR, and a
279 o environmental concerns associated with the presence of toxic metals, these quantum dots are not wel
280                             We confirmed the presence of Trem2-dependent DAM and identified a previou
281 -mediated STAT1 phosphorylation required the presence of TRIM6.
282 east Saccharomyces cerevisiae growing in the presence of two carbon sources.
283 was achieved by electropolymerization in the presence of two computationally selected and cysteine mo
284 rom UBA III into UBA I still occurred in the presence of UBA III seeds.
285 and randomly diffuse on DNA, however, in the presence of UV-induced DNA lesions these complexes stall
286                                          The presence of van der Waals interactions can be identified
287                                          The presence of variants in genes associated with monogenic
288 iated mobilization of U as a function of the presence of various essential plant nutrients contained
289 nding to SHBG was reduced by ~20-fold in the presence of zinc, whereas DVT binding was almost complet
290  The cellular lineages identified by pigment presence or absence (wild-type or knock-out lineages, re
291 ntestinal biopsies, with gammac cytokines in presence or absence of BNZ-2.
292 recombination dynamics depend largely on the presence or absence of C(60).
293 cupine's digital bits are represented by the presence or absence of distinct DNA strands, called mole
294 a with CAF expanded similarly in mice in the presence or absence of docetaxel.
295                                          The presence or absence of PSB then determines whether the t
296  for p53 activation, Atg7 was deleted in the presence or absence of the master regulator of antioxida
297  predict glycan subtype, thus predicting the presence or absence of this virulence marker.
298 t robust and consistent predictor of disease presence (or absence) compared to alpha-diversity indice
299 etion with anti-CD20 to retain B cells whose presence were required to achieve EAE recovery.
300 een minimum temperature and Brazilian pepper presence, with a discrete threshold temperature occurrin

 
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