ライフサイエンスコーパス: presequence

1 ytoplasm without a conventional mt-targeting presequence.

2 ed M(r) of 52,000 containing a 31-amino acid presequence.

3 a preprotein with a 50-amino acid N-terminal presequence.

4 its native form by removal of the N-terminal presequence.

5 and it recognizes a mitochondrial targeting presequence.

6 s of mature QPs3 and a 55-amino acid residue presequence.

7 ed to a size consistent with cleavage of the presequence.

8 includes a putative chloroplastic targeting presequence.

9 ibiting a step other than proteolysis of the presequence.

10 uits other motor subunits and binds incoming presequence.

11 matrix, based on an N-terminal, amphipathic presequence.

12 and function of the 20-amino acid N-terminal presequence.

13 l step in import, i.e., translocation of the presequence.

14 rane by a bipartite, cleaved, amino-terminal presequence.

15 inner membrane Tim17/23 translocon by their presequences.

16 ssessing independent mitochondrial targeting presequences.

17 residues and contain 9-amino-acid cleavable presequences.

18 thesized as precursors that carry N-terminal presequences.

19 ns targeted by positively charged N-terminal presequences.

20 preprotein maturation, beyond the removal of presequences.

21 (1) protein has been shown to lack a cleaved presequence; a single methionine is removed from the ami

22 teins carry a positively charged, N-terminal presequence and are imported by the TIM23 complex (prese

23 c, allowing recognition of both the incoming presequence and other translocase components at the tran

24 ows that MPP recognizes a second site in the presequence and processing occurs between residues 43 an

25 is not imported using a conventional cleaved presequence and show that sequences at the N-terminus of

26 efore MIP cleaves off the second part of the presequence and that this second processing step takes p

27 oteins of the intermembrane space (IMS) lack presequences and are imported in an oxidation-driven rea

28 e two major IMS-targeting signals, bipartite presequences and cysteine motifs.

29 d us to propose that the length of targeting presequences and the subunit composition of organellar p

30 s are targeted to mitochondria by N-terminal presequences and use the TIM23 complex for their translo

31 independent of proteolytic processing of the presequence, and that o-phenanthroline together with EDT

32 tructure unveils how mitochondrial targeting presequences are recognized.

33 These presequences are rich in positive charges, mainly argini

34 reveals the highly conserved nature of such presequences, as well as of the import machinery.

35 The cleavable presequence at the amino terminus of the precursor form

36 tates along with Abeta- and citrate synthase presequence-bound PreP at 3.3-4.6 angstrom resolution.

37 the Deltapsi is not linked to the respective presequence, but rather to the mature portion of the pol

38 ired in TOM-TIM23 coupling and the import of presequence-carrying preproteins.

39 can assume a conformation incompatible with presequence cleavage.

40 al peptide are important for Som1p-dependent presequence cleavage; however, no specific cargo sequenc

41 ing plasmids lacking either the IDH1 or IDH2 presequence coding regions were unexpectedly found to be

42 ecursors have functionally similar bipartite presequences composed of an N-terminal signal peptide do

43 lly required for the import of mitochondrial presequence-containing but not for carrier proteins.

44 ing experiments indicate that the N-terminal presequence-containing domain of the substrate proteins

45 Here, we report that import of presequence-containing precursors and carrier proteins i

46 termed the TIM23 complex, mediates import of presequence-containing proteins (preproteins) into the m

47 0 along with Tim23 regulate translocation of presequence-containing proteins across the mitochondrial

48 brucei The results show that ATOM46 prefers presequence-containing, hydrophilic proteins that lack t

49 As shown herein, the presequence contains significant secondary structure and

50 ransit peptides, and processing of the LAP-N presequence could generate the mature 55-kD LAP-N.

51 Only 24% of carriers met EHR-based presequencing criteria for probable or definite FH diagn

52 sparagine-linked glycosylation reporters and presequence deletion constructs of the precursor to the

53 , while shorter hydrogenosomal and mitosomal presequences did not.

54 al target presequence of 64 residues and the presequence directed the green fluorescent protein to to

55 The bipartite presequences directing the products of these genes back

56 The presequence dramatically altered SCP-2 structure as dete

57 x) sorts precursor proteins with a cleavable presequence either into the matrix or into the inner mem

58 We propose that the presequence either targets Atp6p to the Atp9p or signals

59 The 16-amino acid mitochondrial targeting presequence, encoded by the second and third exons, was

60 his study, we applied a diverse selection of presequencing enrichment methods targeting all major vir

61 h subunit was found to be dependent upon its presequence for mitochondrial localization, and the subu

62 gulator2 homolog (SRT2) contains a predicted presequence for mitochondrial targeting.

63 her CCS nor SOD1 contains typical N-terminal presequences for mitochondrial uptake; however, the mito

64 ity of mitochondrial proteins use N-terminal presequences for targeting to mitochondria and are trans

65 id N-terminal presequence that is similar to presequences found on all examined hydrogenosomal protei

66 dria, even after fusion of the mitochondrial presequence from pmAAT to its N-terminal end.

67 -directed mutagenesis was used to remove the presequences from each gene and from both genes carried

68 st three peptidases are involved in cleaving presequences from imported mitochondrial proteins.

69 was shown by expressing the LipDH N-terminal presequences fused to green fluorescent protein in eryth

70 ssed a natural substrate, pre-23K, and a 23K presequence-green fluorescent protein (GFP) chimera in v

71 The LAP-A presequence had features similar to chloroplast transit

72 The 5-kD presequence had features similar to plastid transit pept

73 ex we used iron-sulfur proteins in which the presequences had been changed by site-directed mutagenes

74 nosomal AAC contains a cleavable, N-terminal presequence; however, this sequence is not necessary for

75 ta indicate the importance of the N-terminal presequence in regulating SCP-2 structure, cholesterol l

76 his study has examined the role of the Atp6p presequence in the function and assembly of the ATPase c

77 PP), is capable of cleaving a hydrogenosomal presequence in vitro, in contrast to MPP which requires

78 of Sf9 cells and required the mitochondrial presequences in both subunits.

79 ial proteins are synthesized with N-terminal presequences in the cytosol.

80 sized as preproteins carrying amino-terminal presequences in the cytosol.

81 studies of Pex5p(C) binding], the N-terminal presequence increased SCP-2's affinity for Pex5p(C) by 1

82 Inspection of the LAP-A presequence indicated the presence of a dibasic protease

83 most of the import of preproteins containing presequences into the mitochondria, and its primary stru

84 Translocation of the presequence is an early event in import of preproteins a

85 rt competence can be retained as long as the presequence is capable of forming a relatively more stab

86 If proline-20 of the S. pombe presequence is changed into a serine, a second cleavage

87 onversely, if serine-24 of the S. cerevisiae presequence is changed to a proline, the first cleavage

88 The mutated presequence is cleaved and forms mature-sized protein in

89 uggesting that the N-terminal portion of the presequence is essential for membrane binding.

90 surface is dramatically accelerated when its presequence is long enough to span both membranes and to

91 cates that the precursor of Tic22 (preTic22) presequence is necessary and sufficient for targeting to

92 ges in the N-terminal helical segment of the presequence is necessary for import competence.

93 sults indicate that removal of the subunit 6 presequence is not an essential event for ATPase biogene

94 ate of DeltapH-driven translocation, and the presequence is optimized in its natural acidic residue c

95 icate that a single amino acid change in the presequence is responsible for one-step processing in S.

96 If the presequence is too short, import is slow but can be stro

97 rotein and the COOH-terminal residues of its presequence is used as a substrate.

98 d us to postulate that the N-terminus of the presequence is vital for import and the C-terminal end i

99 brane domains (TMDs), whereas ATOM69 prefers presequence-lacking, hydrophobic substrates that have TM

100 Specifically, longer mitochondrial presequences may have evolved to require an alpha/beta h

101 inducible biosensor for monitoring the main presequence-mediated import pathway with a quantitative,

102 role as a "ticket canceller" for removal of presequences, MPP exhibits a second conserved activity a

103 long 105-amino acid mitochondrial targeting presequence (mTP).

104 domain hydrophobic region oriented with the presequence N terminus formed by signal peptidase cleava

105 (NTD) is intrinsically disordered and binds presequence near a region important for interaction with

106 presequence of the ribosomal protein L29 and presequence of 1-aminocyclopropane-1-carboxylic acid dea

107 rame of 2634 nucleotides encoding a putative presequence of 31 amino acid residues and a mature prote

108 A peptide presequence of 52 amino acids is cleaved to give the mat

109 ed a typical N-terminal mitochondrial target presequence of 64 residues and the presequence directed

110 lacking negative charges in the unstructured presequence of LF(N) translocate independently of the De

111 The latter effect depends on the presequence of LMP7, but not on LMP7 catalytic activity.

112 st for the first time that the 20-amino acid presequence of pro-SCP-2 alters SCP-2 structure to facil

113 these positive charges in the 19-amino acid presequence of rat liver aldehyde dehydrogenase was inve

114 from the first 25 amino acid residues in the presequence of subunit IV of yeast cytochrome c oxidase

115 The mitochondrial targeting presequence of the "Rieske" protein (subunit 9) is lodge

116 N- and C-terminal fragments derived from the presequence of the ATPase beta subunit ranging in size f

117 We previously reported that the presequence of the F(1)-ATPase beta subunit precursor (p

118 e cytochrome bc1 complex, we mutagenized the presequence of the iron-sulfur protein to eliminate the

119 s, respectively, in the cleaved NH2-terminal presequence of the precursor protein (pre-LMP3), and six

120 Short mitochondrial targeting peptides (presequence of the ribosomal protein L29 and presequence

121 asts of a chimeric protein consisting of the presequence of the small subunit of ribulose 1,5-bisphos

122 imeric precursor proteins, consisting of the presequence of the small subunit of Rubisco fused to the

123 ue peptide derived from the membrane-binding presequence of yeast cytochrome c oxidase, and the pepti

124 On the basis of the unique presequences of the nuclear genes and the recent mitocho

125 In contrast, the mitochondrial targeting presequences of these same proteins have not undergone a

126 The mitochondrial targeting presequences of two sdh3 genes are derived from preexist

127 rt rates of artificial precursors containing presequences of varying length fused to either mouse dih

128 eting to the endoplasmic reticulum (ER), the presequence on mitochondrial proteins is inhibited from

129 ese observations suggest that the N-terminal presequence on the avidin-bound precursor is available f

130 otranslational import requires an N-terminal presequence on the nascent protein and contributes to lo

131 ed question in gene evolution: the origin of presequences or transit peptides that generally exist in

132 orm of TFAM, which retains the mitochondrial presequence, ordinarily removed upon mitochondrial impor

133 hierarchical dominance of the mitochondrial presequence over the NLS.

134 ensable for general protein import along the presequence pathway, we show that it participates in the

135 The protein analytes were positively charged presequences (pb2) of varying length fused to the small

136 f mitochondrial Abeta-degrading enzyme PreP (presequence peptidase) was enhanced in Tg mAPP mitochond

137 etic peptide corresponding to the 29-residue presequence peptide (mAAT-pp) with anionic phospholipid

138 could be re-assigned and characterized as a presequence peptide degrading enzyme in the matrix.Prote

139 om40 constructs specifically interact with a presequence peptide in a concentration- and voltage-depe

140 sistent with only a shallow insertion of the presequence peptide in the bilayer.

141 supported by the fact that the addition of a presequence peptide induces transient pore closure.

142 Based on these results, we propose that the presequence peptide may contain dual recognition element

143 no acid sequence, indicating a mitochondrial presequence peptide of only nine amino acids.

144 channel in the presence of the mitochondrial presequence peptide pF(1)beta revealed the kinetics of p

145 nnel formation or for the interaction with a presequence peptide.

146 o not, however, alter the activity of PSC, a presequence-peptide sensitive channel in the mitochondri

147 tal for mitochondrial proteostasis, degrades presequence peptides cleaved off from nuclear-encoded pr

148 Cleaved presequence peptides then need to be efficiently degrade

149 s not significantly blocked by mitochondrial presequence peptides.

150 uded that positively charged residues in the presequence play a vital role in the import of precursor

151 m the mature protein portion and less on the presequence portion, when a synthetic peptide composed o

152 Analysis of the presequence predicts that the protein is targeted to out

153 Although the 20-amino acid presequence present in 15-kDa pro-sterol carrier protein

154 Phosphorylation of this presequence prevents mitochondrial import and directs TF

155 ial clearance against impaired mitochondrial presequence processing and proteotoxic stress.

156 al syndrome whereby defects of mitochondrial presequence processing induce an early activation of UPR

157 ure the correct functioning of the essential presequence processing machinery.

158 the pathogenetic mechanisms of mitochondrial presequence processing, we employed cortical neurons and

159 Presequence protease (PreP), a 117 kDa mitochondrial M16

160 Presequence protease (PreP), a mitochondrial peptidasome

161 gradation of targeting peptides catalyzed by presequence protease.

162 A role for the cargo in presequence recognition distinguishes Imp1p and Imp2p fr

163 otosystem II (pLHCPII) were used to identify presequence regions translocated into the ER lumen and s

164 y brominated phospholipids reveals that this presequence residue inserts to a depth of approximately

165 of the peptide corresponding to the R3Q/R10Q presequence revealed that this peptide was only somewhat

166 of hydrogenosomal and mitochondrial protein presequences reveals striking similarities.

167 These results suggest that the presequence segment lies close to the surface of the mem

168 peptide corresponding to the linker-deleted presequence showed that it was substantially more prone

169 Analysis of presequence structure and in vitro import experiments in

170 Analysis of presequence structure and in vitro import into microsoma

171 They have an N-terminal presequence that allows recognition by the mitochondrial

172 to precursors with an NH2-terminal targeting presequence that are imported in a linear NH2-terminal m

173 ine-rich, 69-residue mitochondrial targeting presequence that is absent in the mature protein.

174 synthesized with an N-terminal 10-amino acid presequence that is cleaved during assembly of the compl

175 e presence of an eight amino acid N-terminal presequence that is similar to presequences found on all

176 as a preprotein with a cleavable N-terminal presequence that is the mitochondrial targeting signal,

177 ino-acid amino-terminal, organelle-targeting presequence that was cleaved in vivo.

178 their respective organelles using N-terminal presequences that are subsequently cleaved by a peptidas

179 tions of three peptides, which correspond to presequences that direct mitochondrial protein import, w

180 ny mitochondrial proteins contain N-terminal presequences that direct them to the organelle.

181 interaction, including the length of the pb2 presequence, the position of the electrostatic traps wit

182 Tim23p does not carry an amino-terminal presequence; therefore, the targeting information reside

183 ith the structural similarity of all Euglena presequences, these results demonstrate that chloroplast

184 to drive translocation of positively charged presequences through the TIM23 complex (presequence tran

185 of a conserved leucine at position 2 in the presequence to a glycine disrupts import of pFd into the

186 ct on the affinity or mode of binding of the presequence to model membranes.

187 The presequence translocase (TIM23 complex) sorts precursor

188 TIM23 complex, functions as a gatekeeper of presequence translocase and thereby maintains quality co

189 The presequence translocase complex, translocase of the inne

190 The presequence translocase constituent Pam17 is specificall

191 nclude that Mgr2 is a coupling factor of the presequence translocase crucial for cell growth at eleva

192 The presequence translocase of the inner membrane (TIM23 com

193 of the outer mitochondrial membrane and the presequence translocase of the inner membrane.

194 The presequence translocase of the inner mitochondrial membr

195 NT complex is required for inhibition of the presequence translocase TIM23, which leads to stabilizat

196 uence and are imported by the TIM23 complex (presequence translocase) located in the inner mitochondr

197 rged presequences through the TIM23 complex (presequence translocase), the activity of the Hsp70-powe

198 chaperone cooperates with cochaperones, the presequence translocase, and other chaperone systems.

199 Loss of MMP, but not the presequence translocase-associated import motor (PAM), b

200 ates with partner protein complexes like the presequence translocase-associated import motor and the

201 across the inner membrane is provided by the presequence translocase-associated motor (PAM) which con

202 a through interactions with the import motor presequence translocase-associated motor (PAM).

203 ormal channel activity and disruption of the presequence translocase-associated motor complex did not

204 from the channel to the import motor of the presequence translocase.

205 to mitochondria and are translocated by the presequence translocase.

206 nown energy-dependent steps: Deltapsi-driven presequence translocation and adenosine triphosphate-dri

207 The mitochondrial presequence translocation machinery (TIM23 complex) is c

208 ot modify TIM23 channel activity nor prevent presequence translocation.

209 With precursors that have sufficiently long presequences, unfolding by the inner membrane import mac

210 This presequence was necessary to target Hsp60 to one (and oc

211 , whose sequences mimic mitochondrial import presequences, was measured using a novel, qualitative, f

212 the putative mitochondrial matrix targeting presequences) were co-expressed in Escherichia coli on o

213 are imported from the cytosol via N-terminal presequences, which are cleaved upon exposure to the mit

214 east promoters and a mitochondrial targeting presequence with the bacterial coding sequence.