ライフサイエンスコーパス: primase

1 inding activity of D5, the poxvirus helicase-primase.

2 Hence, DnaC controls the access of DnaB by primase.

3 nto the mechanism of nucleotide synthesis by primase.

4 (p180DeltaN-p70) inhibited RNA synthesis by primase.

5 Interestingly, Ctf4 binds only one Pol alpha-primase.

6 t a new primer synthesized downstream by the primase.

7 ific DNA recognition in an archaeoeukaryotic primase.

8 lves interactions of these proteins with DNA primase.

9 ific DNA recognition in an archaeoeukaryotic primase.

10 hat of a functional primer synthesized by T7 primase.

11 interaction of the yeast ortholog pol1 with primase.

12 tacting and sequestering the relaxase-linked primase.

13 d, pre-translocation state competent to bind primase.

14 80 catalytic subunit of DNA polymerase alpha-primase.

15 e interface in the NTD of DnaB that contacts primase.

16 ymerase requires RNA primers produced by DNA primase.

17 on due to impaired binding to both ssDNA and primase.

18 s to elevated RNA primer synthesis by T7 DNA primase.

19 bility of this DNA helicase to interact with primase.

20 ructure of the iron-sulfur cluster domain of primase.

21 occluding this region from interacting with primase.

22 main not found in the archaeal and bacterial primases.

23 and eukaryotes requires the activity of DNA primase, a DNA-dependent RNA polymerase that lays short

24 all organisms depends on the activity of DNA primase, a DNA-dependent RNA polymerase that synthesizes

25 CST/AAF, a DNA polalpha.primase accessory factor, binds POT1b and shortens the e

26 The molecules were docked to the primase active site using the available primase crystal

27 iously unrecognized as critical to the human primase active site.

28 been reported to stimulate the helicase and primase activities of the complex in the presence of ICP

29 The helicase and primase activities of the hexameric ring-shaped T7 gp4 p

30 nzymes, PrimPol possesses DNA polymerase and primase activities that are important for replication fo

31 expresses full and functional helicase (and primase) activities when bound to a gp61 primase subunit

32 ase activity, it substantially augments both primase activity and primase-to-polymerase switching.

33 Furthermore, we show that primase activity exhibits a cooperative dependence on pr

34 Biochemical assays measuring primase activity have been limited to monitoring formati

35 additionally report that although PrimPol's primase activity is required to restore wild-type replic

36 We find that primase activity lowers replisome processivity but only

37 r Mn(II) over Mg(II), suggesting that T7 DNA primase activity modulation when bound to Mn(II) is base

38 sis reveals that RPA serves to stimulate the primase activity of PrimPol.

39 the discontinuous nature of DNA replication, primase activity on the lagging strand is required throu

40 e N-terminal domain in T7 gp4 contains a DNA primase activity, this function is lost in metazoan mtDN

41 th the second subunit (p180C-p70) stimulated primase activity, whereas the whole catalytically active

42 y DNA polymerase-alpha and its intrinsic RNA primase activity.

43 activity, and PrimPol, a DNA polymerase with primase activity.

44 s zinc ions and is essential for maintaining primase activity.

45 he primosome, but significantly inhibits its primase activity.

46 n has helicase activity but no DNA-dependent primase activity.

47 e (PrimPol; CCDC111), an archaeal-eukaryotic primase (AEP) in eukaryotic cells, is involved in chromo

48 is article focuses on the archaeo-eukaryotic primase (AEP) superfamily, drawing on recently character

49 In eukaryotes, a single archaeo-eukaryotic primase (AEP), DNA primase, is required for the initiati

50 Slow primer release from DNA primase allows the polymerase to engage the complex and

51 olecule inhibitors of the activity of T7 DNA primase, an ideal model for bacterial primases due to th

52 -stranded DNA de novo, all organisms require primase, an RNA polymerase making short RNA primers whic

53 s predicted to possess an archaeo-eukaryotic primase and a UL52-like zinc finger domain, the role of

54 lication system of bacteriophage T7 both DNA primase and DNA helicase activities are contained within

55 human primosome, a 340-kilodalton complex of primase and DNA polymerase alpha (Polalpha), synthesizes

56 bserve that signal release is independent of primase and does not seem to require a protein trigger a

57 Disrupting the interaction between primase and helicase in Escherichia coli increases Okaza

58 alled "clamp zones." Loading depends on DnaG primase and is probably driven by Okazaki fragment initi

59 ack of crystal structures of the full-length primase and its complexes with substrates in initiation

60 and is consistent with the requirements for primase and ligase activities as well as earlier electro

61 ost recently discovered human DNA polymerase/primase and plays an emerging role in nuclear and mitoch

62 e ability to disrupt the association between primase and pol alpha allowed us to assess the physiolog

63 The catalytic subunits of primase and pol alpha synthesize composite RNA-DNA prime

64 domains and defining the requirement for its primase and polymerase activities during nuclear DNA rep

65 s, we find that SSBs significantly limit the primase and polymerase activities of PrimPol.

66 that PrimPolY89D has a striking decrease in primase and polymerase activities.

67 een p180C, p70, and p58 regulates the proper primase and polymerase function.

68 PrimPol was recently identified as a TLS primase and polymerase involved in DNA damage tolerance.

69 However, the negative effects of primase and rNTPs on processivity are overcome by the ex

70 New herpesvirus drugs include viral helicase-primase and terminase inhibitors.

71 lication proteins, including DnaA, helicase, primase and the clamp loader, TrfA interaction with the

72 ivity in replication and show that Pol alpha-primase and the lagging-strand Pol delta can be re-used

73 xtension effected by cooperation between DNA primase and the lagging-strand polymerase.

74 nts and binding mode for interactions of DNA primase and thymidylate synthetase (TS) with high and lo

75 l is a recently discovered DNA-dependent DNA primase and translesion synthesis DNA polymerase found i

76 aryotic AEP-like proteins with DNA-dependent primase and/or polymerase activity.

77 is novel assay should be applicable to other primases and inefficient DNA/RNA polymerases, facilitati

78 the HU-14 noncoding region between dnaG (DNA primase) and rpoD (sigA).

79 rations of DNA polymerase-alpha primase (Pol-primase), and the p58 subunit of Pol-primase associates

80 and V that also function with the helicase, primase, and sliding clamp in the replisome.

81 ed residues within the ATPase, Topoisomerase/Primase, and Winged helix domains, including four that e

82 The redox switch in eukaryotic DNA primases appears to regulate polymerase handoff, and in

83 n reaction catalyzed by the T. kodakaraensis primase are discussed.

84 olymerase and the zinc-finger domains of DNA primase are involved in the stabilization of the priming

85 that the dramatic conformational changes in primase are necessary to accomplish the initiation and t

86 Primases are crucial RNA polymerases that perform the in

87 Eukaryotic and archaeal primases are heterodimers consisting of small catalytic

88 Eukaryotic and archaeal primases are heterodimers consisting of small catalytic

89 PRIM1 encoding the catalytic subunit of DNA primase as a novel disease gene.

90 tants A49 and A53 did not interact with UL52 primase as determined by co-immunoprecipitation experime

91 8 of Pol1 abrogates the interaction with the primase, as does mutation to alanine of the invariant am

92 LTA synthases while YvgJ functions as an LTA primase, as indicated by the accumulation of a GroP-Glc(

93 se (Pol-primase), and the p58 subunit of Pol-primase associates with NFIC/CTF1, suggesting that NFI a

94 In eukaryotic cells, the presence of primase at the replication fork is secured by its physic

95 leading Pol epsilon below CMG and Pol alpha-primase at the top of CMG at the replication fork.

96 to assess the physiological significance of primase being tethered to the eukaryotic replisome in th

97 lusters using DNA charge transport regulates primase binding to DNA and illustrates chemistry that ma

98 at occur only on the lagging strand, such as primase binding to DnaB helicase, RNA synthesis, and SS

99 revealed that the N-terminal domain of UL52 primase binds UL5 helicase and the middle domain interac

100 r binds weakly to fork DNA in the absence of primase, but forms a much more stable primosome complex

101 ontrols the ability of DnaB to interact with primase by modifying the conformation of the NTD of DnaB

102 strate that the [4Fe4S] cluster in human DNA primase can make use of this chemistry to coordinate the

103 Here, we show that primase can use metabolic cofactors as initiating substr

104 DNA primases catalyze the synthesis of oligoribonucleotides

105 A primase chimera, where PriX is fused to a truncated vers

106 By contrast, the helicase-primase complex (DnaB and DnaG) remains critically assoc

107 and biochemical characterization of the DNA primase complex and its subunits from the archaeon Therm

108 The Polalpha/primase complex assembles the short RNA-DNA fragments fo

109 The DNA polymerase alpha-primase complex forms an essential part of the eukaryoti

110 The DNA Polymerase alpha (Pol alpha)/primase complex initiates DNA synthesis in eukaryotic re

111 The T. kodakaraensis DNA primase complex is a heterodimer containing stoichiometr

112 es simplex virus 1 (HSV-1) UL5/8/52 helicase-primase complex is required for DNA unwinding at the rep

113 The DNA polymerase alpha-primase complex performs limited synthesis to initiate t

114 We report that the T. kodakaraensis primase complex preferentially interacts with dNTP rathe

115 he completed RNA-DNA primer by the Pol alpha/primase complex simplifies current models of primer tran

116 pplementation of such reactions with the DNA primase complex supported lagging strand formation as we

117 ins including the viral polymerase, helicase-primase complex, and the origin binding protein.

118 itelivir, an inhibitor of the viral helicase-primase complex, exhibits antiviral activity in vitro an

119 e latter findings indicate that the archaeal primase complex, in contrast to the eukaryote homolog, c

120 lished primarily by the DNA polymerase alpha-primase complex, which makes the RNA-DNA primers accessi

121 with a completed Okazaki fragment or primer-primase complexes as the recycling mechanism.

122 ocating along DNA and of helicase-polymerase-primase complexes engaging in synthesis of both DNA stra

123 eriophage DNA replication system that primer-primase complexes have a residence time similar to the t

124 We examined the role of RNA primer-primase complexes left on the lagging ssDNA from primer

125 The collision with primer-primase complexes triggering the early termination of Ok

126 ation machinery includes a trimeric helicase-primase composed of helicase (UL5) and primase (UL52) su

127 Archaea encode a eukaryotic-type primase comprising a catalytic subunit (PriS) and a nonc

128 Prokaryotic primases consist of a zinc-binding domain (ZBD) necessar

129 ically characterized the bacterial-like DnaG primase contained within the hyperthermophilic crenarcha

130 In the complex, Pol alpha and primase cooperate in the production of RNA-DNA oligonucl

131 the primase active site using the available primase crystal structure and ranked based on their pred

132 ion of the essential poxvirus virus helicase-primase D5 and show that the active helicase domain of D

133 vealed that some of the molecules inhibit T7 primase-dependent DNA replication.

134 , which promotes polymerase alpha (polalpha)/primase-dependent fill-in throughout the genome and at t

135 activities such as duplex translocation and primase-dependent RNA synthesis.

136 , we demonstrate redundancy of the Pol alpha-primase DNA polymerase activity in replication and show

137 The phage encodes its own primase, DNA ligase, DNA polymerase, and enzymes necessa

138 short RNA-DNA hybrid primers synthesized by primase-DNA polymerase alpha (Prim-Pol alpha) are needed

139 and purified the previously uncharacterized primase DnaG from Mycobacterium tuberculosis (Mtb DnaG).

140 racts with the replicative helicase DnaC and primase DnaG in a ternary complex.

141 Bacterial DNA primase DnaG synthesizes RNA primers required for chromo

142 e helicase, which is fully suppressed by the primase DnaG.

143 amage in a reaction that is dependent on the primase, DnaG, but independent of any of the known repli

144 A novel peptide from the DNA primase, DNAP(211-223), was also found.

145 e mirrored by experiments in yeast cells, as primase does not interact in cell extracts with pol1 tha

146 The primase domain and the helicase domain were structurally

147 The N-terminal primase domain of the gene 4 protein of phage T7 compris

148 en the winged helix domain and topoisomerase-primase domain, remote from the DNA.

149 ll-length gp4 contains both the helicase and primase domains.

150 T7 DNA primase, an ideal model for bacterial primases due to their common structural and functional f

151 DNA primase facilitates binding of DNA helicase to ssDNA and

152 It tethers Pol alpha and primase, facilitating RNA primer handover from primase t

153 In the 2CMG-Ctf4(3)-1Pol alpha-primase factory model, the two CMGs nearly face each oth

154 th the bacterial DnaG and archaeo-eukaryotic primase families.

155 -polymerase enzyme unrelated to either known primase family.

156 primase function and are applicable for DNA primases from other species.

157 rovide notable insight into the mechanism of primase function and are applicable for DNA primases fro

158 O'Brien et al proposed a novel mechanism of primase function based on redox activity of the iron-sul

159 The role of this region in primase function was further investigated by generating

160 Archaeal organisms contain conserved primase genes homologous to both the bacterial DnaG and

161 ls transfected with WT ICP8 and the helicase-primase (H/P) complex exhibited punctate nuclear structu

162 et al report that the iron-sulfur cluster of primase has a redox role in enzyme activity.

163 zinc-binding domain (ZBD) of prokaryotic DNA primases has been postulated to be crucial for recogniti

164 lthough structures of archaeal and bacterial primases have provided insights into general priming mec

165 ed sites were found within the UL5 (helicase-primase helicase subunit), UL23 (thymidine kinase), UL25

166 nal significance of their interactions using primase, helicase and primer extension assays, and a 'st

167 act with the beta-clamp), in the presence of primase, helicase, Pol III core, clamp loader, and beta-

168 ogy with the bacteriophage T7 gene 4 protein primase-helicase (T7 gp4).

169 Here we characterize a complex between T7 primase-helicase and DNA polymerase on DNA that was trap

170 Whereas one of the polymerases engages the primase-helicase and RNA primer on the lagging strand of

171 omplex consists of two DNA polymerases and a primase-helicase hexamer that assemble on the DNA templa

172 DNA polymerase and multiple subunits of the primase-helicase hexamer.

173 The zinc binding domain of the primase-helicase is essential for trapping the RNA prime

174 These results indicate that the T7 primase-helicase specifically engages two copies of DNA

175 e long 52 gene (UL52; a component of the DNA primase/helicase complex), bICP4, IEtu2, and the unique

176 ding unique long 52 (UL-52; component of DNA primase/helicase complex), Circ, bICP4, and IEtu2 were s

177 Pol-prim is composed of a primase heterodimer that synthesizes an RNA primer, a DN

178 ons in the Polalpha-recruitment and putative primase homology domain in Mcm10/Cdc23 abrogate the ribo

179 quivalent peptide from human Pol alpha binds primase in an analogous fashion.

180 s that the DnaB-DnaC complex binds poorly to primase in comparison with DnaB alone.

181 , we describe the crystal structure of human primase in heterodimeric form consisting of full-length

182 well conserved with heterodimeric (p48/p58) primases in eukaryotes.

183 4-(2-pyridinyl)phenyl ]acetamide, a helicase-primase inhibitor for the treatment of herpes simplex vi

184 Pritelivir is a novel helicase-primase inhibitor in clinical development for treatment

185 ed novel herpes simplex virus (HSV) helicase-primase inhibitor that reduced genital shedding and lesi

186 Helicase-primase inhibitors are novel, potent inhibitors of herpe

187 Pol alpha/primase initiates primers on both strands that are exten

188 ons that inhibit this charge transfer hinder primase initiation without affecting primase structure o

189 to organize two helicases and one Pol alpha-primase into a replication factory.

190 In eukaryotic and archaeal replication, primase is a heterodimer of two subunits, PriS and PriL.

191 The single Pol alpha-primase is centrally located and may prime both sister r

192 harge transfer through the [4Fe4S] domain of primase is not feasible.

193 single archaeo-eukaryotic primase (AEP), DNA primase, is required for the initiation and progression

194 of dATP, glycerol, and Tris buffer, the DNA primase isolated from Thermococcus kodakaraensis catalyz

195 n primosome and the C-terminal domain of the primase large subunit (p58C) with bound DNA/RNA duplex.

196 different proteins containing the helicase, primase, leading polymerase and a lagging strand polymer

197 The LTA primase LtaP(Lm) initiates LTA synthesis by transferring

198 The structures of p48 reveal that eukaryotic primases maintain the conserved catalytic prim fold doma

199 at utilization of cofactors as substrates by primase may influence regulation of replication initiati

200 Here we report the identification of a primase noncatalytic subunit, denoted PriX, from the hyp

201 A deficiency in the primase of bacteriophage T7 to synthesize primers can be

202 utagenesis of the zinc-binding domain of DNA primase of bacteriophage T7 using a bacterial homolog fr

203 ive tryptophan residues are dispersed in the primase of bacteriophage T7: Trp-42 in the ZBD and Trp-6

204 DnaG primase of Escherichia coli initiates synthesis of RNA w

205 two of 12 potential priming sites of the DNA primase of the pRN1 replicon, but nearly all these mutat

206 now be cited demonstrating how the term 'DNA primase' only describes a very narrow subset of these nu

207 olymerase activities resembling those of RNA primases or even canonical RNA-dependent RNA polymerases

208 port through DNA to the [4Fe4S] cluster of a primase p58C construct and a reversible switch in the DN

209 he N-terminal domain of the large subunit of primase (p58N) directly interacts with the C-terminal do

210 support of a functional bacterial-like DnaG primase participating in archaeal DNA replication, we ha

211 onstrated that the presence of a primer, not primase per se, provides the signal that triggers cyclin

212 DNA polymerase alpha-primase (Pol-prim) plays an essential role in eukaryotic

213 t low concentrations of DNA polymerase-alpha primase (Pol-primase), and the p58 subunit of Pol-primas

214 RPA-like ssDNA-binding complex, may regulate primase-Pol alpha (PP) activity at telomeres constitutiv

215 ochemical function of the CST complex is its primase-Pol alpha (PP) stimulatory activity.

216 lls utilise specialized polymerases from the Primase-Polymerase (Prim-Pol) superfamily to maintain ge

217 Eukaryotic Primase-Polymerase (PrimPol) is an enzyme that maintains

218 a multifunctional replicative enzyme called primase-polymerase (PrimPol) that is capable of directly

219 we report that PrimPol, a recently described primase-polymerase (PrimPol), plays a crucial role in th

220 Here, we establish that Primase-Polymerase (PrimPol; CCDC111), an archaeal-eukar

221 The CCPol-MP complex is therefore a unique primase-polymerase enzyme unrelated to either known prim

222 PrimPol is a primase-polymerase found in humans, and other eukaryotes

223 ired for replication initiation, and the DNA primase-polymerase in eukaryotes is pol alpha.

224 PrimPol is a primase-polymerase involved in nuclear and mitochondrial

225 PrimPol was discovered as a primase-polymerase localized to the mitochondria with re

226 ndergo fork reversal in vivo and rely on the primase-polymerase PRIMPOL for repriming, unrestrained r

227 t that engages the repriming activity of the primase-polymerase PrimPol.

228 atory mechanism of this functionally diverse primase-polymerase.

229 In many prokaryotes, a specific DNA primase/polymerase (PolDom) is required for nonhomologou

230 These structures, along with analysis of primase/polymerase activities, provide a plausible mecha

231 ion of these enzymes under a category called primase-polymerases within the wider functional grouping

232 ese findings establish that some replicative primases, previously considered to be solely involved in

233 Here, we report that archaeal replicative primases (Pri S, primase small subunit) can also perform

234 n enzymes: DNA polymerase delta (POLD1), DNA primase (PRIM1), and minichromosome 6 (MCM6).

235 kaging tegument protein), and UL52 (helicase-primase primase subunit) proteins.

236 ng activity of the bacteriophage T4 helicase-primase (primosome) complex.

237 bly, and function of the processive helicase-primase (primosome) component of the bacteriophage T4-co

238 post-replicative gaps formed by the DNA/RNA primase PrimPol.

239 g kinetics with those of the eukaryotic-type primase (PriSL) also found in Sso.

240 ms that guarantee DNA polymerase, clamp, and primase proteins are present for every cycle.

241 DNA primases provide oligoribonucleotides for DNA polymerase

242 ase), we developed the first non-radioactive primase-pyrophosphatase assay.

243 gging-strand polymerases are attached to the primase, ready for Okazaki fragment synthesis in tandem.

244 tations in T7 that suppress the inability of primase reduce the amount of gp5.5 and thus increase the

245 ural differences between bacterial and human primases render the former an excellent target for drug

246 Application of the model to DNA primase revealed a preference in the enzyme's second met

247 e crystal structure of the full-length human primase, revealing the precise overall organization of t

248 ision model) or synthesis of a new primer by primase (signaling model).

249 t that archaeal replicative primases (Pri S, primase small subunit) can also perform TLS.

250 The addition of a single subunit of gp61 primase stabilized the resulting primosome complex at th

251 hinder primase initiation without affecting primase structure or polymerization.

252 ed inside the C-terminal domain of the large primase subunit (p58C).

253 and primase) activities when bound to a gp61 primase subunit at a helicase:primase subunit ratio of 6

254 nits of the helicase hexamer, and the single primase subunit interacts with both strands.

255 Recently, a third primase subunit named PriX was identified in the archaeo

256 While ruling out the role of the primase subunit of Polalpha holoenzyme, we find that mut

257 ound to a gp61 primase subunit at a helicase:primase subunit ratio of 61.

258 A fifth SNV located within UL5 (helicase-primase subunit) greatly reduced in vivo viral replicati

259 rom six (gp41) helicase subunits, one (gp61) primase subunit, and nonhydrolyzable GTPgammaS.

260 s, together with a single tightly bound gp61 primase subunit.

261 The presence of additional primase subunits does not change the molecular mass or h

262 The mode of interaction of primase subunits with substrates during the various step

263 the large subunit of eukaryotic and archaeal primases, suggesting that the PhrB-like photolyases bran

264 As a member of archaeo-eukaryotic primase superfamily enzymes, PrimPol possesses DNA polym

265 l alpha, the critical interaction that keeps primase tethered to the eukaryotic replisome.

266 transcription factor (Mtf1) is an efficient primase that initiates DNA synthesis on ssDNA coated wit

267 synthesis in genomic duplication depends on primase, the DNA-dependent RNA polymerase that synthesiz

268 ulating the activity of DNA polymerase-alpha primase, the only enzyme known to initiate DNA replicati

269 DNA replication depends on primase, the specialised polymerase responsible for synt

270 d gp4 lacking the zinc binding domain of the primase; the protein has helicase activity but no DNA-de

271 Once loaded, the helicase recruits the primase through a direct protein-protein interaction to

272 fork construct prior to the addition of the primase to avoid the formation of metastable DNA-protein

273 The ability of T7 DNA primase to catalyze template-directed oligoribonucleotid

274 s an attractive candidate for serving as the primase to initiate lagging strand DNA synthesis during

275 in atomic detail the mode of association of primase to Pol alpha, the critical interaction that keep

276 imase, facilitating RNA primer handover from primase to Pol alpha.

277 and physiological significance of tethering primase to the eukaryotic replisome via pol alpha remain

278 /CTF1, suggesting that NFI also recruits Pol-primase to the NCCR.

279 These findings indicate that tethering of primase to the replisome by pol alpha is critical for th

280 s an oligoribonucleotide, synthesized by DNA primase, to initiate the synthesis of an Okazaki fragmen

281 been thought to require a protein, possibly primase, to pry polymerase from incompletely extended DN

282 tantially augments both primase activity and primase-to-polymerase switching.

283 domains of DNA helicase, five domains of RNA primase, two DNA polymerases, and two thioredoxin (proce

284 ngle-stranded DNA binding protein (UL29) and primase (UL52) genes.

285 icase-primase composed of helicase (UL5) and primase (UL52) subunits and a third subunit, UL8.

286 is revealed that upon binding Mn(II), T7 DNA primase undergoes conformational changes near the metal

287 Primases use single-stranded (ss) DNAs as templates to s

288 DnaB complexed with the C-terminal domain of primase, we found that Ile-85 is located at the interfac

289 ng NADH-quinone reductase subunit A, and DNA primase were expressed in HLA-B27(+) cells, and their HL

290 R screening, fragment molecules that bind T7 primase were identified and then exploited in virtual fi

291 Until relatively recently, DNA primases were viewed simply as a class of proteins that

292 at the RepA CTD interacts with the host DnaG primase, which binds the replicative helicase.

293 cantly enhances the binding of nucleotide to primase, which correlates with higher catalytic efficien

294 ures of the catalytic engine of a eukaryotic primase, which is contained in the p48 subunit.

295 ganisms, DNA replication is initiated by DNA primases, which synthesize primers that are elongated by

296 This direct interaction of a bacterial-like primase with a eukaryotic-like helicase suggests that fo

297 e, as well as the helicase-binding domain of primase with a molar ratio of 6:6:3 at 7.5 A resolution.

298 peptide spanning the last 16 residues binds primase with high affinity, and the equivalent peptide f

299 replicative helicase upon association of the primase with the replisome.

300 has replaced PriL as the subunit that endows primase with the unique ability to initiate nucleic acid

301 The inclusion of multiple DNA primases within a whole domain of organisms complicates