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1 erium Bacillus subtilis, the subject of this Primer.
2 barrier to further elongation of the correct primer.
3 erminus of the FMDV 3D(pol) and RNA template-primer.
4 tch to circulating SARS-CoV-2 in the reverse primer.
5 reactions supplemented with a DENV-specific primer.
6 and nontrivial design of sequence-selective primers.
7 or RT-qPCR evaluation using species-specific primers.
8 n isothermal amplification step and multiple primers.
9 ellaceae, as evidenced by qPCR with 16S rRNA primers.
10 m NGS amplicon sequencing with degenerate PV primers.
11 their DNA accessible to added polymerase and primers.
12 plification and PCR-amplified with universal primers.
13 robustly amplified with extremely conserved primers.
14 environments and using multiple alternative primers.
15 a duplex flanked by single stranded binding primers.
16 o 15 forward primers combined with 4 reverse primers.
17 uC, sitA, tcpC, and terC) not covered by the primers.
20 l changes and energetic cost of bringing the primer 3' end to the priming site are hypothesized to ex
22 imple, reliable, and convenient FAW-specific primers, a pest management programme can be developed no
23 olecular orchestration of regeneration, this Primer aims to showcase both the unique assets and the q
24 in reactions containing either an oligo(dT) primer alone, or in reactions supplemented with a DENV-s
25 focus on the effects of PCR cycle number and primer amplification efficiency on the results of divers
26 ere, we firstly developed a "one-step" tetra-primer amplification-refractory mutation system (T-ARMS)
27 terminus is required for 3B to function as a primer and although a single 3B can support genome repli
30 ol transcribes vRNA using a host-capped mRNA primer and replicates it by synthesizing a positive-sens
31 fluorescence-labeled highly poloymorphic SSR primers and a capillary electrophoresis (CE) detection s
34 of plastidic sorts) and tests with alternate primers and comparisons to metabarcoding did not reveal
37 itute of Technology, synthesized multiplexed primers and probes and formulated a master mix composed
41 extremely slow, and key reagents such as PCR primers and RNA extraction kits are at critical shortage
42 t LiCl-isopropanol DNA precipitation removed primers and small, nonspecific PCR products from live-ce
45 th an ordered series of events that involves primer annealing, genome dimerization, and virion assemb
46 used for fungal classification, but as most primers are genus-specific, it is laborious to identify
47 between genomes in polyploids means that if primers are not homeologue-specific via the incorporatio
48 Here, we report that 3'-amino terminated primers are, in fact, slowly extended by the DNA polymer
50 SPR RNA (crRNA) and isothermal amplification primers, as well as discuss important considerations for
51 h the highest score, as well as all possible primers at every SNP location, are then returned to the
52 how that purified hpol eta adds rNTPs to DNA primers at physiological rNTP concentrations and in the
54 ave an additional 3' extension composed of a primer binding site and a reverse-transcription template
56 enables identification of conserved core PCR primer binding sites flanking polymorphic haplotypes wit
58 id of hpol eta, could not add rNTPs to a DNA primer, but the expression of transfected hpol eta in th
59 tant for prediction and design of probes and primers, but melt curve analyses are low-throughput and
62 e importance of careful attention to method (primer choice, proximal vs. distal location, and develop
69 sus-specific assays that remove the need for primers conventionally targeting variable regions and al
70 y of eDNA in seawater using a broad metazoan primer could identify differences in community compositi
71 promising antibodies, RT-PCR and sequencing primers, CRISPR guides (from research, diagnostics, vacc
73 gets include dengue virus specific consensus primer (DENVCP) and four DNAs corresponding to Staphyloc
75 We showed that the RSV polymerase catalyzed primer-dependent RNA elongation with different lengths o
77 toCloner is the first tool to fully-automate primer design for gene cloning in polyploids, where prev
79 equence knowledge is needed to inform robust primer design when employing PCR amplification, a factor
81 er provides a simple and effective polyploid primer-design method for gene cloning, with no need for
83 developed sRNAPrimerDB, the first automated primer designing and query web service for small ncRNAs.
85 However, inclusion of a DENV-specific cDNA primer did increase the viral genome coverage immediatel
87 (DeltaG), while minimizing the formation of primer-dimers in a pooled experiment, is therefore neces
91 of HCV NS5B in ternary complex with template-primer duplexes and nucleotides, to address the question
92 tch directly from synthetic RNA template/DNA primer duplexes having either a blunt end or a 3'-DNA ov
93 ion is not linked to the use of displacement primers during the initiation of amplification and is th
94 f nascent DNA or spontaneous partitioning of primer-end to the exonuclease site as a "cost of proofre
96 polymerase backtracking, and generate frayed primer-ends that are shuttled to the exonuclease site an
97 developed metagenomic sequencing with spiked primer enrichment (MSSPE), a method for enriching target
99 validity, we design a CRISPR-array-mediated primer-exchange-reaction-based biochemical circuit casca
101 selective 2'-hydroxyl acylation analyzed by primer extension (SHAPE) on in vivo transcripts compared
105 Does the recently illuminated mechanism of primer extension affect the distribution of sequences th
106 selective 2'-hydroxyl acylation analyzed by primer extension and mutational profiling (SHAPE-MaP), u
107 To do this, we developed a high-throughput primer extension assay that allows monitoring of the kin
108 o an agarose bead support enables repetitive primer extension assays for specific genomic DNA targets
115 nonenzymatic template-directed generation of primer extension products long enough to encode active r
116 es are also less efficiently incorporated in primer extension reactions, but the difference is more m
119 ic analysis of reverse transcription and RNA primer extension showed that hpol eta favors the additio
121 good substrate for KOD XL DNA polymerase in primer extension synthesis of modified DNA which exerted
122 phosphate, is shown to be less inhibitory to primer extension than pyrophosphate, the canonical bypro
123 s, while also illuminating the mechanisms of primer extension utilised by closely related Prim-Pols.
126 This mechanism enables Prim-PolC to couple primer extension with template base dislocation, ensurin
136 aternal immune activation (MIA) may act as a primer for multiple genetic and environmental factors to
141 we include a SWEET promoter database, RT-PCR primers for detecting SWEET induction, engineered report
143 lected sequences are employed as water-based primers for PP-metal adhesion to condition PP surfaces a
144 alance between transcription and replication primer formation, as a potential molecular mechanism gov
145 peat 2 (DR2); (iii) DP-rcDNA exhibits an RNA primer-free 5' terminus of (+)strand DNA with either a p
146 n reaction (PCR) amplification, of which 167 primers gave expected PCR amplicon and 101 primers showe
147 ed in cervical cell samples by using general primer GP5+/GP6+-mediated polymerase chain reaction.
148 M392 mutations (M392A, M392L, and M392V) and primer grip mutations (K375R and R376K) exhibited diverg
149 mentarity near the active site, the extended primer grip of the polymerase has the potential to disti
152 ediately adjacent to L420 and the polymerase primer grip region, led us to engineer additional polyme
153 is influenced by its interactions with the "primer grip" region and could be influenced by the M230I
156 XP-V cell extracts did not add dNTPs to DNA primers hybridized to RNA, but could when hpol eta was e
160 easured their relative proportions using the Primer-ID based, quantitative single-variant sequencing
162 ll protein termed VPg or 3B, which acts as a primer in the replication of foot-and-mouth disease viru
164 syltransferases that can elaborate a glucose primer installed onto protein targets by an N-glycosyltr
167 elp readers garner hands-on experience, this Primer is accompanied with tutorial-style code in PyTorc
170 The system uses an inexpensive universal primer labelling system, which can easily be converted t
171 lytic metal ligand, was unable to synthesize primers, likely due to protein instability and weakened
172 ow virus concentration, sequencing coverage, primer mismatches, and replicates influence the accuracy
174 hysical primers which were used as benchmark primers, namely slicing (5.0 +/- 0.6 umol glyceollins/g
176 c cleavage of a test mRNA to generate capped primers of appropriate length and defined 3' terminus in
177 nonspecific endonuclease activity generates primers of appropriate length and specificity during cap
178 he assistance of N protein, generated capped primers of appropriate length and specificity from a tes
179 tween the outbreak strain and the probes and primers of diagnostic assays and the antigenic sites of
181 on of genome-scale enhancer data, we offer a primer on current models of enhancer function in the CNS
182 for neural circuit mapping, and we provide a primer on currently applied anterograde and retrograde v
188 This discussion also serves as a succinct primer on some of the pivotal epidemiological analyses (
189 es on rcDNA 5' termini, specifically the RNA primer on the (+)strand and the polymerase on the (-)str
192 ent RNA elongation with different lengths of primers on both short (10-nt) and long (25-nt) RNA templ
193 ng genera and quantify the bias of barcoding primers on the probability of detection in a natural com
195 rious structures, for example, to remove RNA primers or to produce 3' overhangs at telomeres or doubl
197 dNTP machinery, lead, in the presence of two primers P(1) and/or P(2), to the selective emergence of
198 cation machinery leads, in the presence of a primer, P(1), to the gated replication of the scaffolds
200 ultry and applied it in combination with the primer pair for mammalian species in a duplex assay.
202 g the complement of a 10-23 DNAzyme into the primer pair, PCR-amplified DNAzyme-amps were generated,
204 gions for 48 DNA samples and hundreds of PCR primer pairs (producing up to 23,040 PCR products) durin
205 es were established after screening 526 PLUG primer pairs and performing FISH using oligonucleotides
206 the database included validation against 27 primer pairs from previous studies, a search for serotyp
212 cturally complex NDMMs, some of which act as primer pheromones that are capable of triggering irrever
215 his is the RdRp-SARSr (Charite) confirmatory primer-probe set which has low sensitivity, probably due
217 efficiency and sensitivity, we show that all primer-probe sets can be used to detect SARS-CoV-2 at 50
218 goal was to make independent evaluations of primer-probe sets used in four common SARS-CoV-2 diagnos
221 reaction (RT-PCR), novel quantitative RT-PCR primers/probe were developed, and whole genome sequencin
222 turation, and we find that the efficiency of primer processing by DNA polymerase I in vitro is specif
225 tric polyneuropathy (for the purpose of this Primer, referred to as diabetic neuropathy) is very comm
226 e, we truncated 19 nucleotides (nt) from the primer regions of an 80-nt aptamer, and the resulting 61
227 of constant temperature, use of up to 4 to 6 primers (rendering it highly specific), and capability o
235 validate the frequency's distribution of the primer set, confirming the absence of PCR-based biases.
238 oped in our clinical laboratory based on CDC primer sets and four commercially available, FDA emergen
242 ormed an in silico analysis of 16S rRNA gene primer sets, targeting different hypervariable regions.
245 ilar sensitivities and efficiencies, despite primer site binding mismatches in the Ituri Ebola virus.
248 ll intermediates, in which the transitioning primer strand is stabilized by conserved Pol III residue
255 of POL form an extensive interface with the primer-template and the triphosphate of the incoming dNT
256 by which PrimPol transfers a nucleotide to a primer-template DNA, which comprises DNA binding, confor
257 rase processivity factor, is loaded onto the primer-template junction by replication factor C (RFC).
260 tic and natural templates, extend mismatched primer termini, and bypass secondary DNA structures.
261 RT restrict the movement of the primer, the primer terminus of dsRNA is not blocked from reaching th
262 NA products and to elongate from an unpaired primer terminus opposite template damage, also without t
264 nti-His antibodies, which were modified with primers that induced an isothermal exponential amplifica
266 elements of RT restrict the movement of the primer, the primer terminus of dsRNA is not blocked from
267 Following PCR amplification with indexing primers, the subnucleosome-sized (fragments with a lengt
268 uses complex pools of reverse-transcription primers to enable sequencing enrichment at user-selected
269 CEL-Seq2, in contrast, makes use of poly-dT primers to reverse transcribe mRNA, followed by linear a
270 ites at the template strand and evidence for primer translocation as proposed by the prime-and-realig
271 esion, a suite of siderophore analog surface primers was synthesized with systematic variations in in
294 re, ROS-priming was superior to two physical primers which were used as benchmark primers, namely sli
295 Juglans and the generation of validated SSR primers will be a useful resource for future genetic ana
296 Attribution of these events to particular primers will help inform and improve LAMP primer design.
299 created and evaluated by Primer3; the set of primers with the highest score, as well as all possible
300 ly 0.07% of the taxa detected by a broad COI primer, yet included 43 species not observed in the visu