ライフサイエンスコーパス: primer pair

1 fficient solution-phase PCR using one common primer pair.

2 wing an initial amplification with the G1-G2 primer pair.

3 ngle preparation of genomic DNA and a single primer pair.

4 lification of multiple targets with a single primer pair.

5 d with a consensus and Four Corners-specific primer pair.

6 om this reaction was amplified with a second primer pair.

7 s detected by RT-PCR using a custom-designed primer pair.

8 and replaced with sequences to match any PCR primer pair.

9 sitioning only the perfectly matched cognate primer pairs.

10 ted by a multiplex PCR in this case with 170 primer pairs.

11 ine DNA extraction yielded 17 clone-specific primer pairs.

12 llowed multiplexing with several independent primer pairs.

13 ommercially available multiplex set of eight primer pairs.

14 tranded circular template DNA using specific primer pairs.

15 nce was measured using a qPCR array with 363 primer pairs.

16 ive PCR (QPCR) analysis with genome-specific primer pairs.

17 ing specific polymerase chain reaction (PCR) primer pairs.

18 re developed independently and had different primer pairs.

19 zed by PCR using a set of approximately 6000 primer pairs.

20 virus was detected by PCR using gag-specific primer pairs.

21 which amplify multiple sequences using short primer pairs.

22 using a high-throughput qPCR array with 382 primer pairs.

23 ombination junctions that are not flanked by primer pairs.

24 ar process is used to add probes to selected primer pairs.

25 allowing for simplified selection of optimal primer pairs.

26 ts of these genes, and designs potential PCR primer pairs.

27 lication status of each virus using specific primer pairs.

28 39,000 were validated by e-PCR using unique primer pairs.

29 ajor primer design engine to choose the best primer pairs.

30 We found the popular primer pair 515F (515F-C) and 806R greatly underestimate

31 Using these five primer pairs, 54% of the positive saliva samples were de

32 regions that cannot be amplified by a single primer pair, a directed graph analysis method is used to

33 Two primer pairs (actin and ribonucleotide reductase) were c

34 ix molecular beacons and two oligonucleotide primer pairs, allowing for detection and classification

35 The primer pair also amplified an approximately 370-bp fragm

36 The primer pair amplified a single specific product from eac

37 These primer pairs amplified 94 loci, 57 (61%) of which segreg

38 Thirty-two primer pairs amplified identical fragments from all 44 i

39 fragments from all 44 isolates, while eight primer pairs amplified regions that were polymorphic bet

40 However, when primer pairs amplifying the seven polymorphic AvrII site

41 med by RT-quantitative PCR using a set of 42 primer pairs amplifying var subtype-specific loci coveri

42 A large CISP set (6,062 primer pairs, amplifying introns from 1,676 genes) desig

43 isoform mRNA is quantified using a specific primer pair and a 5'FAM- and 3'TAMARA-labeled probe in c

44 itions for use with the previously described primer pair and determined the limit of detection under

45 s encoded by the 16S rRNA gene serves as the primer pair and is used in combination with both an inte

46 PCR with the SM1-SM4 primer pair and isolated genomic DNA as a template resul

47 ch immunoassay by designing specific labeled primer pair and probe combinations.

48 repeat (SSR) (also known as microsatellite) primer pairs and a plastid DNA deletion marker that dist

49 so carried out regarding the design of NASBA primer pairs and detection probes, as well as reaction a

50 RT-PCR analysis using isoform-specific primer pairs and detector oligonucleotides suggests that

51 irs of primers, including 35 custom-designed primer pairs and four additional primer pairs from the A

52 es were established after screening 526 PLUG primer pairs and performing FISH using oligonucleotides

53 orate additional necessary sequences such as primer pairs and to easily generate files documenting li

54 e the impact of template type, master mixes, primer pairs and, crucially, extraction methods on dPCR

55 As part of the tiling process, primer pairs are computationally screened to meet the cr

56 tally validated primers, only a few thousand primer pairs are currently present in existing databases

57 Finally, both primer pairs are highly sensitive (>99%) in amplifying v

58 Finally, a set of primer pairs are presented for the amplification of the

59 AMP is challenging because a large number of primer pairs are required to detect alterations in the h

60 Primer pairs are then selected computationally to produc

61 for selective amplification, since only the primer pair associated with each amplification need be u

62 In this way a specific hazelnut primer pair based on the ITS marker (70 bp) and a nuclea

63 s of E. canis were amplified by PCR with two primer pairs based on the sequences of E. chaffeensis om

64 ecanucleotides), and the combined ability of primer pairs, based on these oligonucleotides, to detect

65 molecules with ends complementary to a given primer pair but containing nonhomologous central sequenc

66 It not only designs primer pairs but also finds potential isoforms and homol

67 In this report, a set of six novel primer pair combinations, which can be used with previou

68 In total, 240 primer-pair combinations were used to generate >48,000 i

69 The PGMY L1 consensus primer pair combined with the line blot assay allows the

70 etect three common K-ras codon 12 mutations, primer pairs complementary to the wild-type and mutant l

71 amplification is carried out using a generic primer pair corresponding to the oligonucleotide and gen

72 festans RXLR effector clones, obtained using primer pairs corresponding to 32 genes and assigned acti

73 The DNA was amplified with primer pairs covering hypervariable regions of the 16S r

74 In all, 26,855 primer pairs covering most known mouse genes have been e

75 PrimerBank is the experimental validation of primer pairs covering most known mouse genes.

76 analysis is required in order to screen out primer-pair cross interactions.

77 l (LATE)-PCR, solves these problems by using primer pairs deliberately designed for use at unequal co

78 ative PCR of stool DNA with species-specific primer pairs demonstrated significantly reduced levels o

79 ices for the mock community data for the two primer pairs demonstrated that the V3-V4 hypervariable r

80 Ang-1 was confirmed by RT-PCR using specific primer pairs derived from junction sites and the 3' end

81 Over 100 primer pairs derived from noncoding regions of known gen

82 PCR amplification utilizing primer pairs derived from the 5'-UTR reported herein amp

83 rom virus genome DNA by PCR with a consensus primer pair designed from sequences determined for the c

84 The real-time PCR assay uses a single primer pair designed to amplify all known pathogenic RF

85 er sets were identified by serial testing of primer pairs designed from KLKB1 cDNA sequence and DNA s

86 ate mRNA from T560 cells and oligonucleotide primer pairs designed from the published sequence of mou

87 hromosomal hybrid DNAs were PCR amplified by primer pairs designed to the 2p11 reference sequence.

88 Some primer pairs designed were able to amplify all splice va

89 s (verifiable by sequencing) with degenerate primer pairs designed within the butyrate kinase gene or

90 Among the 55 primers pairs designed from clones from pathotype 3 of P

91 itivity, compared to an 87% sensitivity with primer pairs directed to EcoRI fragment D, 32% sensitivi

92 Of 148 specimens, primer pairs directed to the HindIII-X fragment region o

93 ed to EcoRI fragment D, 32% sensitivity with primer pairs directed to the immediate-early antigen 1 g

94 1 gene (IEA1 gene), and 20% sensitivity with primer pairs directed to the major immediate-early (MIE)

95 ed and analyzed by PCRs with three different primer pairs directed toward bacterial rRNA loci.

96 eviously, we demonstrated the utility of two primer pairs, directed to the rRNA operon, to specifical

97 Overall, this primer pair displayed a sensitivity and specificity of 9

98 For two of these ESTs we tested fourteen primer pairs each and using standard optimization method

99 rect assay that amplifies the 16S rRNA gene (primer pair ehr521-ehr790; detects 0.25 infected cell).

100 Each primer pair either specifically amplified a DNA fragment

101 ave an option to add a probe to any selected primer pair, enabling design of Taqman and other primer-

102 At present, 531 306 and 2 941 669 primer pairs exist across 223 species for miRNAs and piR

103 been designed and in many cases alternative primer pairs exist.

104 Most primer pairs facing in opposite directions gave several

105 the ADH1 and MATalpha loci by PCR, using the primer pairs flanking the Ho cut sites.

106 silosis sensu lato FKS1 gene and a universal primer pair followed by EcoRI enzyme digestion.

107 ultry and applied it in combination with the primer pair for mammalian species in a duplex assay.

108 We designed a novel primer pair for poultry and applied it in combination wi

109 a lower annealing temperature or a different primer pair for the HIV-1 pol region.

110 Primer pairs for 314 SSLP markers were designed and type

111 We list primer pairs for amplifying each exon, with flanking int

112 d that ensures the design of target-specific primer pairs for DNA amplification.

113 esource for the retrieval of human and mouse primer pairs for gene expression analysis by PCR and Qua

114 n software application that designs multiple primer pairs for generating PCR probes useful for fluore

115 e fragments to the Primer3 program to design primer pairs for PCR amplification.

116 xperimentally validated collection of murine primer pairs for PCR and QPCR which can be used under a

117 e designed a "one-tube test" comprising four primer pairs for polymorphic tetranucleotide repeat sequ

118 e used a SmartChip quantitative PCR with 382 primer pairs for profiling the resistome and MGEs and 16

119 pairs for sequences on chromosome 18, three primer pairs for sequences on chromosome 13, and one pri

120 tide repeat sequences on chromosome 21, four primer pairs for sequences on chromosome 18, three prime

121 ies; these regions were used to design three primer pairs for species-specific PCR.

122 Intron sequences were used to design primer pairs for the amplification of all exons.

123 Primer pairs for the amplification of each of the 13 p73

124 thms designed to select and match degenerate primer pairs for the amplification of viral genomes.

125 was to compare published Helicobacter pylori primer pairs for their ability to reliably detect H. pyl

126 es were used to design 13 taxon-specific PCR primer pairs for those taxa most commonly implicated in

127 We designed 33,179 primer pairs from 64,013 SSR motifs.

128 ere reproducibly obtained for seven of eight primer pairs from chromosomal DNA of 33 of the 35 isolat

129 the database included validation against 27 primer pairs from previous studies, a search for serotyp

130 om-designed primer pairs and four additional primer pairs from the ARTIC network protocol v3.

131 One of these primer pairs, G1-G2, only amplified a DNA fragment from

132 sted assay that amplifies the 16S rRNA gene (primer pairs ge3a-ge10 [primary] and ge9-ge3 [nested]; d

133 both populations using previously published primer pairs generated sequences containing multiple sub

134 RNA clone libraries generated with different primer pairs, > 99% and 100% of the sequences were affil

135 For each gene, at least one primer pair has been designed and in many cases alternat

136 The primer pairs have been validated by gel electrophoresis,

137 amplified fragments (AFs) generated with the primer pair HindIII+A and HhaI+A were compared.

138 Five of the twenty-six previously reported primer pairs (HP64-f/HP64-r, HP1/HP2, EHC-U/EHC-L, VAG-F

139 he MY09-MY11 and the GP5(+)-GP6(+) consensus primer pairs, HPV sequences were amplified by nested PCR

140 No combinations of primer pairs improved the results.

141 ragment from the cyt b gene with a universal primer pair in HRM analyses.

142 The presence of a single universal PCR primer pair in solution accelerates amplification within

143 lomerase bypasses a requirement for template-primer pairing in order to add telomeric DNA de novo to

144 re data generated from two platforms for two primer pairs in a low density mock bacterial community.

145 In principle, SDM uses modified primer pairs in a PCR reaction to introduce a mutation i

146 cpz and HIV-1 lineages (groups M, N, and O), primer pairs in conserved pol and env regions were desig

147 n locus (cps) of vaccine serotypes to locate primer pairs in conserved regions that border variable r

148 The assay panel included 16 primer pairs in eight multiplexed reactions and was vali

149 n polymorphism and sequencing with arbitrary primer pairs in molecular population genetics.

150 However, use of a pol primer pair increased the sensitivity of the assay to 98

151 96 clones) using a third universal bacterial primer pair (inosine-341f and 1492r).

152 Primer pairs (intHIV-2/SIV) based on a region of integra

153 sequence-based method employing a single PCR primer pair is cost-effective and simple.

154 The complex primer pair is covalently tethered through its 5' end to

155 A variant-specific primer pair is required to amplify each alternatively sp

156 However, designing subtype-specific PCR primer pairs is a very challenging task: on one hand, se

157 PCR with single primer IS3A and primer pair IS3A-IS3B identified 6 and 14 different subt

158 Amplification of adjacent primer pairs, labeled in two colors, allowed us to narro

159 orghum RIL mapping population using the same primer pairs located approximately 200 of the BAC contig

160 e chain reaction (RT-PCR) was performed with primer pairs located in exons flanking the exon (extra d

161 iii) an M. catarrhalis-specific primer pair (primer pair MCA1-MCA2).

162 plifies the major surface protein gene msp2 (primer pair msp2-3f-msp2-3r; detects 0.25 infected cell)

163 very challenging task: on one hand, selected primer pairs must result in robust amplification in the

164 We have developed a PCR primer pair, named EBIEF1/EBIER1, based on the small-sub

165 We propose that seven core SSR primer pairs, namely, SMC31CUQ, SMC336BS, SMC597CS, SMC7

166 sequenced and then used to design a specific primer pair, Nf1 (16-mer) and Nf2 (16-mer), complementar

167 However, real-time PCR using gene-specific primer pairs only amplified Ee-BAM1, indicating that Ee-

168 y conserved crRNA targets from user-provided primer pairs or PrimedRPA output files.

169 reaction with genomic DNA, single primers or primer pairs oriented in the same direction showed negli

170 PsASGR-BABY BOOM-like (psASGR-BBML)-specific primer pair p779/p780 was in perfect linkage with the AS

171 g the complement of a 10-23 DNAzyme into the primer pair, PCR-amplified DNAzyme-amps were generated,

172 The SlipChip was designed to preload one primer pair per reaction compartment and to screen up to

173 Detection limits of the 26 PCR primer pairs previously described for detection of H. py

174 h DG74, and (iii) an M. catarrhalis-specific primer pair (primer pair MCA1-MCA2).

175 Redesign of six of the primer pairs (Primer set_B), re-genotyping of the succes

176 gments were amplified with several different primer pairs prior to cloning and sequencing or communit

177 The new primer pair/probe set detected 140 of 141 isolates, incl

178 ersion of the AMPLICOR HIV-1 test, and a new primer pair/probe system.

179 gions for 48 DNA samples and hundreds of PCR primer pairs (producing up to 23,040 PCR products) durin

180 In addition, the 16S primer pair putatively identified B. cepacia in seven pa

181 btained different statistical outcomes among primer pairs, raising unexpected but serious questions a

182 Minimally, testing numerous primer pairs seems to be necessary to avoid false conclu

183 se chain reaction (RT-PCR) using a published primer pair sequence followed by restriction endonucleas

184 ts, we designed 410 evolutionarily conserved primer pair sequences which are specific for anchor locu

185 The use of SuperSelective primer pairs should enhance the sensitivity of multiplex

186 Two of the primer pairs showed 100% sensitivity and specificity for

187 tent with the primer extension results, some primer pairs showed no amplification, indicating that th

188 Amplification with the same primer pairs showed strong concordance within each platf

189 Compared with other PCR-based methods, this primer pair shows a higher efficiency of detection in di

190 reparation, PCR-based amplification with the primer pair SK39 (biotinylated at the 5' end) and SK38,

191 is of cps from 92 serotypes indicated that a primer pair spanning the regulatory gene cpsB could puta

192 ite polymerase chain reaction (PCR) using 15 primer pairs spanning the common deleted region on 20q s

193 rate PCR reactions, each of which contains a primer pair specific to one or the other allelic SNP var

194 amplified by polymerase chain reaction using primer pairs specific for the DQB loci followed by South

195 he HLA DR loci (n = 1) or inability to obain primer pair specificity among similar HLA DR types (n =

196 Successful cross-amplification for 12 primer pairs studied in detail using DNA from downy mild

197 h multiplex PCR derived from the use of many primer pairs such as false amplicons, primer-dimers etc.

198 re evaluated with a previously described gag primer pair system.

199 The design of panels of hundreds of primer pairs takes just hours using this program, compar

200 reverse transcription primers, specific PCR primers pairs, TaqMan probe, double-hairpin probe and hy

201 n for all the genomovars indicated that this primer pair targeted a region shared by these isolates.

202 a given environment by amplifying eDNA using primer pairs targeting a wide range of taxa and high-thr

203 Eight different primer pairs targeting different variable regions of the

204 We amplified eDNA using 12 primer pairs targeting mammals, fish, amphibians, birds,

205 Six mini-barcode primer pairs targeting short (127-314 bp) fragments of t

206 We provide a database of over 4.3 million primer pairs targeting the human and mouse genomes that

207 Analysis by the PCR/ESI-MS method using nine primer pairs targeting the most information-rich regions

208 considered a common feature of PCR, 30 of 32 primer pairs tested in this study produced primer-dimer

209 le product), determined that of the fourteen primer pairs tested, six and nine pairs respectively amp

210 Here we present a primer pair that eliminates this problem, allowing simpl

211 The conclusions were validated by using primer pairs that amplify sequences within the cystic fi

212 Indel Group in Genomes (IGG) markers are primer pairs that amplify single-locus sequences that di

213 The assay utilizes 24 primer pairs that amplify targets in the mtDNA control r

214 Ten primer pairs that anneal upstream and downstream of each

215 endonuclease restriction maps, we chose PCR primer pairs that approximated and framed junctions crea

216 ate the list of candidate primers, to choose primer pairs that are best suited for their needs.

217 regions to allow design of a limited set of primer pairs that can selectively target coding regions

218 In this work, we identified four PCR primer pairs that collectively amplified cdt genes in al

219 mark PCR Arrays from SuperArray, containing primer pairs that have been experimentally validated to

220 and conformation polymorphism analysis using primer pairs that span the BRCA1 coding region and intro

221 RT-PCR using primer pairs that were designed to detect specifically n

222 Using two primer pairs that will either specifically amplify the l

223 Based on complementary primer pairs, the QuickChange method has been widely use

224 r binding regions used for the MY09 and MY11 primer pair, this PCR assay detects the presence of 27 H

225 t from thousands of computationally proposed primer pairs those that are specific to individual rdh g

226 Using a specific primer pair to amplify Nanog by reverse transcriptase-PC

227 airs for sequences on chromosome 13, and one primer pair to identify the sex chromosomes.

228 sed RealSeqS, a technique that uses a single primer pair to interrogate ~350,000 genome-spanning regi

229 e chain reaction assay that employs the same primer pair to simultaneously amplify ERalpha and ERbeta

230 quential amplification reactions with nested primer pairs to achieve the necessary specificity and se

231 We designed PCR primer pairs to amplify genomic DNA flanking each of 40

232 region of the mutation by using intron/exon primer pairs to amplify regions of the precursor nuclear

233 med tetra-primer ARMS-PCR, which employs two primer pairs to amplify, respectively, the two different

234 onstrate this principle, we designed 384 PCR primer pairs to conserved exonic regions flanking intron

235 d on the 384-well SlipChip with 20 different primer pairs to identify 16 bacterial and fungal species

236 Here we present a combination of eight primer pairs to identify mold within a single PCR run.

237 This allowed the design of taxon-specific primer pairs to quantify the abundance of the most repre

238 LST housekeeping gene loci by using the same primer pairs used in MLST.

239 ber of different bands detected for each SSR primer pair using a LI-COR- DNA Analyzer ranged from two

240 lification of multiple targets with a single primer pair, using MLPA probes containing unique barcode

241 tions than does another previously described primer pair, V1/EB450.

242 Two primer pairs (W3F-W4R, W3F-W2R) were used in separate po

243 coding success rate found with an individual primer pair was 88.6% compared to 20.5% success rate ach

244 A single primer pair was designed to amplify a 348-bp region of s

245 An outer primer pair was designed to amplify an approximately 340

246 previous studies in which only a single PCR primer pair was used may have significantly underestimat

247 A novel PCR primer pair was used to detect the presence of cmeC in 1

248 A total of 3607 non-redundant SSR primer pairs was designed based on the sequences of CDS,

249 One of the primer pairs was further assessed for species specificit

250 nformation was unavailable, selection of PCR primer pairs was guided by general insights gleaned from

251 Using a single primer pair, we amplified ~350,000 amplicons distributed

252 Using a single primer pair, we generated amplicons of high sequence com

253 lymorphism fingerprinting technique with two primer pairs, we identified 12 genotypes among the isola

254 0 degrees C annealing temperature or the pol primer pair were 71 and 98%, respectively.

255 A set of four species-specific real-time PCR primer pairs were also designed, capable of detecting le

256 Primer pairs were designed for more than 60,000 SSR-cont

257 Multiple primer pairs were designed from the sequence of a 200 kb

258 ping among legumes; thus 60 of the EST-based primer pairs were designed to amplify orthologous sequen

259 Primer pairs were designed to span five different nucleo

260 Primer pairs were designed to span introns for polymorph

261 Further, virus-specific primer pairs were identified for California encephalitis

262 A total of 318 primer pairs were optimized for domestic cats, and 86% o

263 Two primer pairs were putatively specific for B. cepacia.

264 The primer pairs were separated and collected by IP-RP HPLC.

265 of quantitation using PCR utilizing dual LUX primer pairs were similar to quantitation using single L

266 A total of 187 primer pairs were tested under five different abiotic st

267 These primer pairs were tested with 44 O157 isolates, 2 each f

268 The mini-barcode primer pairs were then tested against 44 processed fish

269 Nine primer pairs were used to generate AFLP patterns, with a

270 Degenerative primer-pairs were first designed based on the highly con

271 confound standard-design "RNA-specific" PCR primer pairs which rely, for cDNA versus gDNA specificit

272 Here, seven primer pairs (which amplify within the loci 16S rRNA, Rv

273 f this product was used to design a specific primer pair, which generated a single 864-bp fragment wi

274 native and innovative strategy is to utilize primer pairs with 3'-overhangs, but this approach has no

275 tional and real-time PCR for a subset of 112 primer pairs with a success rate of 98.2%.

276 temperature for each primer and selects the primer pairs with comparable annealing temperature.

277 dal B cell lymphomas have employed different primer pairs with detection sensitivities varying betwee

278 A number of different primer pairs with homology to most known genital HPV typ

279 ompartment and to screen up to 384 different primer pairs with less than 30 nanoliters of sample per

280 e RNAs, provided that the 3' terminus of the primer pairs with the template.

281 es, or anchored PCR, using C region-specific primers paired with vector specific primers.

282 Primer pairs within the 3' untranslated region of PCTA-1