ライフサイエンスコーパス: primitive streak

1 to disrupted germ-layer morphogenesis at the primitive streak.

2 ientation of mesoderm cells as they exit the primitive streak.

3 ts are inhibited and cells accumulate in the primitive streak.

4 erm cells after their ingression through the primitive streak.

5 abrogates normal cell migration through the primitive streak.

6 rsors located in the epiblast lateral to the primitive streak.

7 in E-cadherin protein downregulation in the primitive streak.

8 necessary for migration of mesoderm from the primitive streak.

9 e epiblast resulting in the formation of the primitive streak.

10 however, were those expressed in vivo in the primitive streak.

11 ls at stages leading to the formation of the primitive streak.

12 rm, a derivative of the middle region of the primitive streak.

13 or the proper morphogenesis of the mammalian primitive streak.

14 n the limb bud as well as axial mesoderm and primitive streak.

15 er maintained at a defined distance from the primitive streak.

16 elop beyond 14 days or the appearance of the primitive streak.

17 y as a mesodermal extension of the posterior primitive streak.

18 ntains the remnants of Hensen's node and the primitive streak.

19 ent as they underwent ingression through the primitive streak.

20 ctivated but is faithfully maintained in the primitive streak.

21 ectively, inhibit or induce formation of the primitive streak.

22 ists between the left and right sides of the primitive streak.

23 ial for the normal formation of the node and primitive streak.

24 tial for HNF3beta expression in the anterior primitive streak.

25 hric or mesonephric precursor cells from the primitive streak.

26 l array extending the complete length of the primitive streak.

27 istance from the organizer at the tip of the primitive streak.

28 the trunk organizer in the node and anterior primitive streak.

29 mesodermal cells are not recruited into the primitive streak.

30 distant from the signal, to form an ectopic primitive streak.

31 1 and Wnt8C, is located in the middle of the primitive streak.

32 ectoderm is dependent on derivatives of the primitive streak.

33 is required for cell migration away from the primitive streak.

34 ), prior to formation of the fully elongated primitive streak.

35 lecules BMP4 and Vg1/GDF1 in positioning the primitive streak.

36 subsets, although it is not detected in the primitive streak.

37 et of gastrulation with the formation of the primitive streak.

38 population found at the anterior end of the primitive streak.

39 he anterior visceral endoderm and the distal primitive streak.

40 ption of signalling activity in the anterior primitive streak.

41 senchymal transition and ingress through the primitive streak.

42 sed signalling cues from areas including the primitive streak.

43 sendoderm, which is a major component of the primitive streak.

44 vely uncoupling it from the induction of the primitive streak.

45 ally deleted from the migrating mesoderm and primitive streak.

46 mesoderm precursors to migrate away from the primitive streak.

47 he embryo and a gain of Wnt signaling in the primitive streak.

48 a specific progenitor population in the late primitive streak.

49 ix adhesion or by signals from the posterior primitive streak.

50 al-to-mesenchymal transition at the anterior primitive streak.

51 By stage 6, shortly after cells leave the primitive streak, a field of cells is generate which is

52 er and from other more caudal regions of the primitive streak act on the rostral prospective neuroect

53 to the normal derivatives of the definitive primitive streak along its rostrocaudal extent and which

54 monstrated that Alk2 mutant embryos formed a primitive streak, although abnormally thickened, and wer

55 ouble homozygous mutants fail to establish a primitive streak, although the anterior visceral endoder

56 and exhibit an accumulation of cells at the primitive streak and a selective loss of paraxial mesode

57 g between extrinsic mechanisms involving the primitive streak and an intrinsic role for the allantois

58 rovide evidence that Wnt3a, expressed in the primitive streak and dorsal posterior node, acts as a lo

59 s axial symmetry, and induces markers of the primitive streak and epithelial-to-mesenchymal transitio

60 pression is first detected at stage 3 in the primitive streak and exhibits transient low-level expres

61 ing event that leads to the formation of the primitive streak and gastrulation.

62 nd foregut lining, emerges from the anterior primitive streak and Hensen's node as a cell monolayer t

63 E-cadherin needs to be downregulated in the primitive streak and in the epiblast, concomitant with t

64 The establishment of the primitive streak and its derivative germ layers, mesoder

65 tion of progenitors initially located in the primitive streak and later in the tail bud.

66 he epiblast is required for induction of the primitive streak and mesoderm whereas activity in the po

67 ed visceral endoderm and subsequently to the primitive streak and mesoderm.

68 ting that loss of pluripotency, formation of primitive streak and mesodermal lineage progression are

69 gene family that is highly expressed in the primitive streak and migrating mesoderm cells on embryon

70 Cre identifies a requirement for Zic3 in the primitive streak and migrating mesoderm for proper left-

71 However, the defects in both the lulu primitive streak and neural plate are associated with di

72 iblast, and subsequently is expressed in the primitive streak and newly formed embryonic mesoderm.

73 The primitive streak and node become disfigured, consistent

74 on of Wnt-3a and Wnt-8c is restricted to the primitive streak and posterior lateral plate, and is abs

75 In the mouse, Tbx6 is expressed in the primitive streak and presomitic mesoderm, and is sharply

76 embryonic polarity: it helps to position the primitive streak and some have suggested that it might a

77 ryonic day 7 (E7) in the neural ectoderm and primitive streak and subsequently in the brain, peripher

78 use embryogenesis, Gdf11 is expressed in the primitive streak and tail bud regions, which are sites w

79 nitor population transcriptomes in the mouse primitive streak and tail bud throughout axial elongatio

80 of resident cells located in the regressing primitive streak and tail bud.

81 bilaterally to the nerve cord as a result of primitive streak and tail-bud regression during body axi

82 uction pathways that direct cell fate in the primitive streak and tailbud of the early embryo.

83 Zeb2 repress P-cadherin transcription in the primitive streak and the neural plate, respectively.

84 ion system to examine formation of the chick primitive streak and to test the proposal that oriented

85 he caudal presomitic mesoderm (PSM) from the primitive streak (and later the tail bud), while somites

86 , shortly after its gastrulation through the primitive streak, and earlier than previously reported.

87 l of individual cells in the mouse epiblast, primitive streak, and early YS.

88 s expressed in inner cell mass cells and the primitive streak, and later is restricted to the develop

89 , Cr-1 is expressed in the mouse blastocyst, primitive streak, and later is restricted to the develop

90 tributes prospective endodermal cells to the primitive streak, and subsequently to definitive endoder

91 ibutions mainly from the rostral half of the primitive streak, and that endodermal movements parallel

92 al end of the embryo, whereas markers of the primitive streak are absent or localized to the proximal

93 migration of nascent mesodermal cells in the primitive streak as the morphogenetic basis underlying t

94 nally different from that coordinated by the primitive streak, as it does not lead to mesodermal diff

95 To gain insight into primitive streak assembly and function, we have conducte

96 Impaired primitive streak assembly in the mouse amnionless (amn)

97 y in size after E7.0, indicating that middle primitive streak assembly is mechanistically tied to the

98 We propose that these cells arise within primitive streak-associated epiblast via a mechanism tha

99 ough required, does not autonomously promote primitive streak-associated gene expression in vitro.

100 of Wnt activity promotes restriction towards primitive streak-associated lineages with mesendodermal

101 ome cells in lulu mutants are trapped in the primitive streak at an intermediate stage of the epithel

102 of Pten null embryos express markers of the primitive streak at ectopic locations around the embryon

103 The mouse posterior primitive streak at neural plate/headfold stages (NP/HF,

104 tly of mesendoderm formation to position the primitive streak at the midline.

105 Importantly, the node and primitive streak at these stages lack Cx43 mRNA.

106 he anterior-most somites ingress through the primitive streak before E7 and migrate anteriorly by E7.

107 Epiblast cells adjacent to the regressing primitive streak behave as a stem zone that progressivel

108 s ingressing through the anterior and middle primitive streak, but it does not affect EMT of cells lo

109 th the replacement of the regressed node and primitive streak by a caudal blastema-like mass of mesen

110 at Dact1 contributes to morphogenesis at the primitive streak by regulating Vangl2 upstream of cell a

111 eriments in the mouse have revealed that the primitive streak can be divided into three functional re

112 As reported previously, an ectopic primitive streak can be induced by misexpression of Vg1

113 d/4) that lack Hensen's node (organizer) and primitive streak can reconstitute a functional organizer

114 ssion, which leads to Fgf4 expression in the primitive streak, can also promote mesoderm migration in

115 ls into mesodermal subtypes that reflect mid-primitive-streak cardiogenic mesoderm and posterior-prim

116 d specification of primordial germ cells and primitive streak cells.

117 erm was defined by following labeled rostral primitive-streak cells over a short period of time as th

118 The acquisition of primitive streak characteristics by self-renewing EpiSCs

119 r subfraction of cells with reversible early primitive streak characteristics, which is mutually excl

120 gesting correspondence to gastrulation-stage primitive streak, chorion and allantois precursors, resp

121 orcine PGCs originate from the posterior pre-primitive-streak competent epiblast by sequential upregu

122 epithelial to mesenchymal transition in the primitive streak, correct patterning of mesoderm, and lo

123 hird signal, FGF4, which is expressed in the primitive streak, defines an inductive center for hindbr

124 Formation of the node, at the anterior primitive streak, depends on expression of the transcrip

125 mbryos also display an expansion of anterior primitive streak derivatives and anterior neurectoderm t

126 ng allantois, a rudimentary heart and middle primitive streak derivatives such as somites and lateral

127 involved in the convergence of visceral and primitive streak-derived endoderm.

128 sults indicate that Lim1 is required in both primitive streak-derived tissues and visceral endoderm f

129 aembryonic anterior visceral endoderm and in primitive streak-derived tissues of early mouse embryos.

130 initiates a process that resembles posterior primitive streak development in a SNAI1-dependent manner

131 lance between BMP and Nodal signaling during primitive streak development, and provide a potential me

132 trulation, when cells ingressing through the primitive streak differentiate into the precursor cells

133 irects FAST site-dependent expression in the primitive streak during gastrulation, and unilateral exp

134 so plays a role in cell movement through the primitive streak during gastrulation.

135 delay and an accumulation of mesoderm in the primitive streak during gastrulation.

136 long-range migration after emerging from the primitive streak during gastrulation.

137 sors called hemangioblasts, specified in the primitive streak during gastrulation.

138 cells in various rostrocaudal levels of the primitive streak during key periods in early development

139 As the primitive streak elongates, this tissue with organizing

140 hat oriented cell division could account for primitive streak elongation.

141 We propose that the amniote primitive streak evolved from the ancestral blastopore b

142 xed, and blood island precursor cells in the primitive streak express many of the same molecular mark

143 ions on the posterior side are essential for primitive-streak extension during gastrulation by render

144 ring gastrulation, FOXF1 marks all posterior primitive streak extra-embryonic mesoderm derivatives wi

145 he mouse embryo before the appearance of the primitive streak; first in the posterior visceral endode

146 Smad2 signals serve to restrict the site of primitive streak formation and establish anterior-poster

147 mouse embryos serve to restrict the site of primitive streak formation and establish anteroposterior

148 Gata2 influences the site of primitive streak formation and its role is independent f

149 red for anterior-posterior (A-P) patterning, primitive streak formation and left-right (L-R) axis det

150 (mesd) functional interval is essential for primitive streak formation and mesoderm induction.

151 4 (BMP-4) and a BMP antagonist, chordin, in primitive streak formation and neural induction in amnio

152 al programs similar to those observed during primitive streak formation and subsequent gastrulation i

153 of BMP signalling by chordin plays a role in primitive streak formation and that chordin is not suffi

154 tigate the signalling pathways that initiate primitive streak formation and the mechanisms that ensur

155 nce of the marginal zone to initiate ectopic primitive streak formation in response to cVg1 is depend

156 isexpressed in the posterior area pellucida, primitive streak formation is inhibited.

157 In the mouse, primitive streak formation is the first overt morphologi

158 Subsequently, just prior to the time of primitive streak formation, a conformational change in t

159 eptors are required for egg cylinder growth, primitive streak formation, and rostral head development

160 both BMP-4 and chordin are expressed before primitive streak formation, and that BMP-4 expression is

161 g of the visceral endoderm is independent of primitive streak formation, but the subsequent establish

162 controls epiblast cell movements leading to primitive streak formation, generating bilateral symmetr

163 Primitive streak formation, however, was impaired in chi

164 lso show that FGF signalling is required for primitive streak formation, in cooperation with Nodal an

165 function in the posterior epiblast promotes primitive streak formation, whereas transient nodal expr

166 last of chick embryos in the early stages of primitive streak formation.

167 extinguished in the epiblast at the onset of primitive streak formation.

168 spatially directed cytokineses during early primitive streak formation.

169 ited polarized cell divisions during ectopic primitive streak formation.

170 the presumptive neural plate before or after primitive streak formation.

171 in the epiblast is quite uniform before the primitive streak forms then decreases in the central epi

172 analyses, suggested a defect in the anterior primitive streak, from which much of the embryonic mesod

173 e early gastrula they are located in the mid-primitive streak, from which they enter the mesoderm bil

174 When the primitive streak has reached its full length, a later gr

175 us origins and developmental pathways of the primitive streak have been proposed.

176 ve-streak cardiogenic mesoderm and posterior-primitive-streak hemogenic mesoderm.

177 Chicken Wnt11b is expressed in the primitive streak in a pattern similar to chicken Wnt5a a

178 Initiation of the primitive streak in avian embryos provides a well-studie

179 area pellucida enables this region to form a primitive streak in response to cVg1.

180 ion of mesoderm cells ingressing through the primitive streak in the chick embryo.

181 tissue flow underlying the formation of the primitive streak in the chick embryo.

182 ive neuroectoderm immediately rostral to the primitive streak in the early gastrula.

183 n and avian embryos is the appearance of the primitive streak in the future posterior region of a rad

184 and ingression of mesoderm cells through the primitive streak, including fibroblast growth factors an

185 on of mesenchymal cells around the shortened primitive streak indicating a functional requirement of

186 est frequency in the posterior region of the primitive streak, indicating that initial stages of haem

187 Wnt antagonists Crescent and Dkk-1 block the primitive streak-inducing ability of cVg1 in the margina

188 During mammalian embryonic development, the primitive streak initiates the differentiation of plurip

189 hat Pten is not required in the cells of the primitive streak; instead, Pten is required for normal m

190 First, they emerge from the primitive streak into the region of the endoderm that fo

191 mechanisms that mediate the assembly of the primitive streak into these functional regions, we have

192 ve proximal ACD and intraembryonic posterior primitive streak (IPS) contributed to a wide range of so

193 f prospective paraxial mesoderm cells to the primitive streak is delayed.

194 8 expression in nascent mesoderm exiting the primitive streak is dramatically reduced in mutant embry

195 k stages XI-XII through HH stage 3, when the primitive streak is initially established and prior to t

196 in other vertebrates as it suggests that the primitive streak is initially established in a radial pa

197 The primitive streak is the defining feature of the gastrula

198 y is required for cell migration through the primitive streak, is a target of miR-130b and -218 in vi

199 ior epiblast, and hence the formation of the primitive streak, is the result of local cell-cell inter

200 a late inhibition from Lefty emitted by the primitive streak itself.

201 astrulation, ingressing through a "bilateral primitive streak" just inside the blastopore.

202 rcation event early in the trajectory when a primitive streak-like cell population segregated into th

203 s described here, hPSCs are first induced to primitive streak-like cells by activating canonical Wnt

204 says and embryo grafting we demonstrate that primitive streak-like EpiSCs are biased towards mesoderm

205 Generation of a primitive streak-like population in embryoid bodies, fol

206 coding genes, associated with pluripotency, primitive streak, limb development and extracellular mat

207 ation, Rac1Deltaepi embryos have an enlarged primitive streak, make only a small amount of paraxial m

208 eak border) in which ectoderm that expresses primitive streak markers overlies the prospective notoch

209 to a linear transition in the expression of primitive streak markers precedes the formation of the p

210 gulates its own expression and that of other primitive streak markers via activation of the canonical

211 ferentiation, accelerating the expression of primitive streak markers.

212 circle and a ring of mesendoderm expressing primitive-streak markers in between.

213 The primitive streak marks the midline of the future embryo.

214 hematopoietic progenitors without affecting primitive streak mesoderm formation.

215 Cell migration, which is characteristic of primitive streak mesoderm in vivo, was not induced by FG

216 ting cells in both the progress zone and the primitive streak mesoderm suggests that one function of

217 for FGF signaling in the induction of mouse primitive streak mesoderm, as well as in later patternin

218 e progressive loss of paraxial, lateral, and primitive streak mesoderm.

219 ranscription factor that is expressed in the primitive streak, mesoderm and anterior mesendoderm of t

220 elop a normal egg cylinder but do not form a primitive streak, mesoderm or node.

221 Furthermore, we show that FGF4 expressed in primitive streak-mesoderm can induce the differentiation

222 ing the basement membrane of the prospective primitive streak more prone to breaching.

223 somite progenitor cells ingress through the primitive streak, move laterally to a paraxial position

224 at E7.5 with defects in the formation of the primitive streak, node, and mesoderm.

225 crease in canonical Wnt activity in the late primitive streak of all Axin2(canp) mutant embryos that

226 to the chick, as inhibition of K(ATP) in the primitive streak of chick embryos randomizes the express

227 day 8.5 (E8.5) embryos and the head fold and primitive streak of E7.5 embryos.

228 Brachyury mRNA expression in the primitive streak of RIalpha mutants is significantly red

229 3a encodes a signal that is expressed in the primitive streak of the gastrulating mouse embryo and is

230 F family members that are coexpressed in the primitive streak of the gastrulating mouse embryo.

231 ed primary body) through the activity of the primitive streak on axial progenitors within the epiblas

232 streak markers precedes the formation of the primitive streak on one side of the epiblast.

233 mechanisms that mediate the assembly of the primitive streak or about the signaling pathways that sp

234 rally believed to initiate expression at the primitive streak or early neural plate stages.

235 sis suggests that the hESCs progress through primitive streak or mesendoderm to mesoderm, before diff

236 imitive endodermal cell layers, but lacked a primitive streak or recognizable mesoderm.

237 ay 7 of gestation, fail to develop mesoderm, primitive streak, or proamniotic cavity.

238 First, we determined the primitive-streak origin of the endoderm using supravital

239 leads to gastrulation phenotypes, including primitive streak patterning defects in association with

240 or low levels of activin-induced a posterior primitive streak population, whereas high levels of acti

241 with high levels of Gdf11 expression in the primitive streak, presomitic mesoderm, and tail bud.

242 cs, BMP and Wnt initially specified anterior primitive streak (progenitor to endoderm), yet, 24 hr la

243 ion in the anterior visceral endoderm (AVE), primitive streak (PS) and definitive endoderm (DE) have

244 Mesoderm is induced at the primitive streak (PS) and patterns subsequently into mes

245 icropattern system to model formation of the primitive streak (PS) by WNT.

246 Abrogation of Smad2 does not compromise primitive streak (PS) formation or gastrulation movement

247 itor based on the temporal expression of the primitive streak (PS) marker brachyury and Flk-1.

248 NMPs are known to reside in the anterior primitive streak (PS) region; however, the extent to whi

249 ells that arise from the anterior end of the primitive streak (PS) to migrate to their correct locati

250 epiblast ingress through a furrow called the primitive streak (PS), and subsequently move away from t

251 tically alters anteroposterior patterning of primitive streak (PS)-like priming.

252 ogether a rudimentary fate map for the human primitive streak (PS).

253 tion, which is initiated by formation of the primitive streak (PS).

254 orphologically stable midline structure, the primitive streak (PS).

255 In mammals, the primitive streak region and its descendant, the tail bud

256 formation and/or specification of the middle primitive streak region during gastrulation.

257 ntify 44 microRNAs that are expressed in the primitive streak region of gastrula stage chicken embryo

258 strulation in the Hoxb1-expressing posterior primitive streak region: Hoxb chromatin was decondensed

259 e mechanisms responsible for positioning the primitive streak remain largely unknown.

260 of stage 4 or 5 quail node into caudal chick primitive streak resulted in the generation of ectopic s

261 with paraxial mesoderm character within the primitive streak, resulting in a lateral expansion of so

262 IIA(-/-) embryos failed to form an elongated primitive streak, resulting in severe disruption of meso

263 a transcription factor required for anterior primitive streak specification during early development,

264 FOXH1 is a primitive-streak specifier and ACTIVIN co-effector that

265 ximal regions of the egg cylinder at the mid-primitive streak stage (E7.0) with the simultaneous appe

266 more, we show that up to and through the mid-primitive streak stage (i.e., stage 3c/3+), the prospect

267 (i.e., is specified) by the fully elongated primitive streak stage (i.e., stage 3d).

268 lation but are unable to progress beyond the primitive streak stage and die shortly afterward.

269 d-mutant mouse nodes by transplantation into primitive streak stage chick embryos.

270 remarkable capacity to regulate: when a pre-primitive streak stage embryo is cut into fragments, eac

271 The anterior mesendoderm of mid- to late primitive streak stage mouse embryos has the ability to

272 um proteins were synthesized as early as the primitive streak stage.

273 revealed the onset of expression in advanced primitive streak-stage embryos being located in the extr

274 to initiate gastrulation and advance to late primitive streak stages but fail to thrive and are resor

275 n is weakly expressed in the epiblast at pre-primitive streak stages where it is substituted for by P

276 ilure to pattern derivatives of the anterior primitive streak, such as prechordal plate, node, notoch

277 ites derives from stem cells resident in the primitive streak/tail bud, the lateral part derives from

278 n normal spatial domains for Cdx expression (primitive streak/tailbud), yet, overall, they contain el

279 otes the expression of genes in the anterior primitive streak that are important for the development

280 anterior area pellucida generates an ectopic primitive streak that expresses mesoderm and organizer m

281 Formation of the primitive streak, the equivalent of the blastopore, is a

282 curs through an axial midline structure, the primitive streak, the formation of which is preceded by

283 lthough Wnt5a and Wnt5b are expressed in the primitive streak, the known Wnt11 gene is expressed in p

284 osterior of GCNF(-/-) embryos, including the primitive streak, the node, and the presomitic mesoderm.

285 While tissue is still in the primitive streak, the prospective IM is relatively uncom

286 all are associated with abnormalities in the primitive streak, the site of the EMT.

287 ll elongation and alignment upon leaving the primitive streak, the subsequent polarized protrusive ac

288 n the amniote begins with the formation of a primitive streak through which precursors of definitive

289 hordoneural hinge were grafted to 8.5 d.p.c. primitive streak to compare their developmental potency.

290 strulation, epiblast cells delaminate at the primitive streak to form mesoderm and definitive endoder

291 During mouse gastrulation, cells in the primitive streak undergo epithelial-mesenchymal transfor

292 No significant contribution to the early primitive streak was seen from the anterolateral epiblas

293 f Fgf8 and study its role in lineages of the primitive streak, we have used a new mouse line, T-Cre,

294 dye labeling, precursor cells of the stage 3 primitive streak were mapped predominantly to a specific

295 is required for cell migration away from the primitive streak when gastrulation initiates, but previo

296 n Dact1 mutants, Vangl2 was increased at the primitive streak, where cells ordinarily undergo an epit

297 ion displays characteristics of the anterior primitive streak, whereas the CD4-Foxa2(lo)GFP-Bry(+) ce

298 descendants of 8.5 d.p.c. node and anterior primitive streak which remain in the tail bud are locate

299 al edge of the isolate forms a reconstituted primitive streak, which gives rise to the normal derivat

300 can reconstitute a functional organizer and primitive streak within 10-12 hours in culture.