ライフサイエンスコーパス: pro-TRH

1 All pro-TRH neurons receiving contacts by alpha-MSH-containi

2 with in situ hybridization revealed that all pro-TRH mRNA-positive neurons in these ventral medullary

3 We analyzed pro-TRH-mRNA expression, mapped TRH-immunoreactive eleme

4 h an anatomical association between CART and pro-TRH-producing neurons in the PVN and demonstrate tha

5 veal the morphological relationships between pro-TRH mRNA-containing neurons and CART- and alpha-MSH-

6 did not augment the ability of PC2 to cleave pro-TRH to either N- or C-terminal forms.

7 itivity of prothyrotropin-releasing hormone (pro-TRH) gene expression in the paraventricular nucleus

8 Rat prothyrotropin-releasing hormone (pro-TRH) is endoproteolyzed within the regulated secreto

9 Prothyrotropin-releasing hormone (pro-TRH) is initially cleaved by the prohormone converta

10 The prothyrotropin-releasing hormone (pro-TRH) precursor (26 kDa) undergoes proteolytic cleava

11 lymerase chain reaction (qRT-PCR) identified pro-TRH transcript in a number of different brain region

12 ddition to catecholamine and angiotensin II, pro-TRH/TRH may be another important axis that affects h

13 roid axis can affect metabolism, deficits in pro-TRH processing may contribute to the obese and diabe

14 dependently stimulated a 10-fold increase in pro-TRH biosynthesis, with a maximum response at 10 nm.

15 The finding that TRbeta2 localized in pro-TRH-synthesizing neurons in the ventral medullary nu

16 convertase, and particularly PC1 and PC2, in pro-TRH processing, recombinant vaccinia viruses were us

17 thyroid hormone receptor beta2 (TRbeta2) in pro-TRH-synthesizing neurons in the Rpa, Rob and the PPR

18 n hypophysiotropic TRH neurons by increasing pro-TRH gene expression and the biosynthesis of TRH.

19 in the mechanism by which leptin influences pro-TRH gene expression.

20 situ hybridization with digoxigenin-labeled pro-TRH cRNA probe.

21 llular subdivisions of the PVN and to 34% of pro-TRH neurons in the medial parvocellular subdivision,

22 ties were juxtaposed to approximately 70% of pro-TRH neurons in the anterior and periventricular parv

23 ART was co-contained in approximately 80% of pro-TRH neuronal perikarya, whereas colocalization with

24 Transcript abundance of pro-TRH can be increased in cultured cardiac fibroblasts

25 H has an important role in the activation of pro-TRH gene expression in hypophysiotropic neurons via

26 Concentrations of pro-TRH forms are increased in homozygotes.

27 re, we show that after MI, the expression of pro-TRH is induced in the heart coordinately with the pr

28 When a mutant form of pro-TRH, which has the dibasic sites of initial processi

29 -IR axons densely innervated the majority of pro-TRH mRNA-containing neurons in all parvocellular sub

30 Proteolysis of pro-TRH begins in the trans-Golgi network and forms two

31 s may be necessary for downstream sorting of pro-TRH-derived peptides as it occurs before Golgi exit

32 eam sorting events that result in storage of pro-TRH-derived peptides in mature secretory granules.

33 3 d prevented fasting-induced suppression of pro-TRH in the PVN but had no effect on AGRP mRNA in the

34 ART prevented fasting-induced suppression of pro-TRH in the PVN when administered intracerebroventric

35 Our strategy was to block the transport of pro-TRH or its intermediates from one subcellular compar

36 ted that initial processing action of PC1 on pro-TRH in the trans-Golgi network, and not a cargo-rece

37 Partial pro-TRH transcript from C. batrachus transcriptome showe

38 inant PC1 and PC2 process partially purified pro-TRH to cryptic peptides in vitro and that pro-TRH an

39 whereas neuropeptide Y treatment suppressed pro-TRH biosynthesis approximately 3-fold.

40 utively secreted form of PC1 does not target pro-TRH peptides to the constitutive secretory pathway b

41 Furthermore, the C-terminal pro-TRH-derived peptides were more efficiently released

42 ro-TRH to cryptic peptides in vitro and that pro-TRH and PC1 mRNAs are coexpressed in primary culture

43 In summary, our data show that pro-TRH-derived peptides are differentially sorted withi

44 We now report that when pro-TRH is transiently expressed in GH4C1 cells, a neuro

45 ronal perikarya, whereas colocalization with pro-TRH was found in <10% of the anterior parvocellular

46 y a portion of CART-IR axons in contact with pro-TRH neurons were immunoreactive for alpha-MSH.

47 hed asymmetric synaptic specializations with pro-TRH neurons.