ライフサイエンスコーパス: processive

1 ble-stranded DNA, is that it is slow and non-processive.

2 n form ectopic telomeres if BIR is made less processive.

3 ficiently cross linked, and myosin has to be processive.

4 are sufficiently long that synthesis is more processive.

5 of action: exo, endo-dissociative, and endo-processive.

6 on makes scanning by 48S PIC slower but more processive.

7 h cleaves DNA strands, acting primarily as a processive 5'-3' exonuclease and secondarily as a 5'-fla

8 Its dominant, processive 5'-3' exonuclease and secondary 5'-flap endon

9 rs based on covalent chemistry and is weakly processive (6 +/- 1 steps per outing).

10 Lin28-stimulated oligo-uridylation, which is processive, a distributive mode is employed by TUT7 for

11 ecommendations for substrate trapping of the processive AAA+ Clp, Lon, and FtsH chaperone proteolytic

12 in which these intermittent dynamics enable processive, accurate, and controllable RNA synthesis is

13 Ena/VASP tetramers are processive actin elongation factors that localize to div

14 Formins are conserved processive actin-polymerizing machines that regulate act

15 Myosin Va (myoVa) is a processive, actin-based molecular motor essential for in

16 differences between the in vitro apparently processive activity and in vivo distributive monouridyly

17 Pauses during processive activity on both types of DNA exhibited ATP d

18 The processive addition and minimal set of PTMs associated w

19 GQ folding of the nascent DNA product during processive addition of multiple telomere repeats modulat

20 lated uracils in the switch region, although processive AID deaminations are evident in some molecule

21 KIF3AC and homodimeric KIF3AA and KIF3CC are processive, although the run length of KIF3AC exceeds th

22 4 (Dis3) and Rrp6 subunits to modulate their processive and distributive 3'-to-5' exoribonuclease act

23 on to facilitate substrate accessibility for processive and efficient CoQ production and distribution

24 almodulin binding protein)) exhibited highly processive and fast motility (up to 0.6 mum s(-1)).

25 model of DNA replication describes a highly-processive and largely continuous process by which the g

26 assays, we show that Kif18B is only modestly processive and that the motor switches frequently betwee

27 Compared with kinesin-1, kinesin-2 is less processive, and its processivity is more sensitive to lo

28 NA synthesis during HR is conservative, less processive, and more mutagenic than replicative DNA synt

29 d direct interactions of cargo with a highly processive anterograde motor.

30 Our recent studies showed that KIF3AC is as processive as conventional kinesin-1, suggesting that th

31 hyscomitrella patens and found that none are processive as native dimers.

32 protein specificity control activities of a processive assembly factor.

33 es Spire from the barbed end to elicit rapid processive assembly from profilin.actin.

34 acer sequences through a mechanism involving processive, ATP-dependent Cas3 translocation along forei

35 eneral design guidelines to construct highly processive, autonomous DNA walker systems and to regulat

36 ophils lacking these components, revealing a processive bleb-based protrusion program that is mechani

37 f DSB resection reveals that AdnAB is highly processive but prone to spontaneous pausing at random si

38 atalyzed by the glycosyltransferase GlfT2, a processive carbohydrate polymerase, which generates a se

39 owth of physiologically compatible bacteria, processive catabolism of complex substrates, and unidire

40 in-recruited ZAP-70, which suggests a weakly processive catalysis on membranes.

41 e enzyme on interfaces and should facilitate processive catalysis.

42 TrCel7A is an exolytic, processive cellobiohydrolase (CBH), with high activity o

43 ts of complexation and decomplexation of the processive cellulase Cel7A and its insoluble substrate,

44 ing partial enzymatic digestion reveals that processive cellulases exploit these defects as initiatio

45 The method was applied to the study of the processive chitinase ChiA from Serratia marcescens.

46 Blunt termini promote processive cleavage, while 3' overhanging termini are cl

47 BLM is significantly less processive compared to the highly efficient GQ destabili

48 Within the complete processive cycle of CBHs, dissociation from the cellulos

49 x may target multiple nucleosomes implying a processive deacetylase function.

50 xhibits a 3' to 5' directional preference in processive deamination.

51 ted nucleotides is not due to events such as processive degradation of nascent DNA or spontaneous par

52 approximately 800 nt gRNA precursors, their processive degradation to a mature size of 40-60 nt, and

53 ation solubilizes its outer surface allowing processive degradation.

54 folding and translocation steps that lead to processive degradation.

55 ased algorithm to classify switching between processive, diffusive, and stuck motility states.

56 igate the movement of fluorescently labeled, processive, dimeric motor proteins and propose a unified

57 ngle molecule analysis reveals that Bad is a processive DNA motor capable of moving along DNA for dis

58 A highly processive DNA polymerase was conjugated to the nanopore

59 eins encircle duplex DNA and are involved in processive DNA replication and the DNA damage response.

60 o be modified as a consequence of disrupting processive DNA replication.

61 sliding clamp), is rapidly exchanged during processive DNA replication.

62 e DNA and associate with DNA polymerases for processive DNA replication.

63 A) adopts a ring-shaped structure to promote processive DNA synthesis, acting as a sliding clamp for

64 w know that PCNA does much more than promote processive DNA synthesis.

65 e and as an essential component required for processive DNA synthesis.

66 le recombinant protein that is functional in processive DNA synthesis.

67 DNAP that can couple strand displacement to processive DNA synthesis.

68 ion of these structures by DDX11 to maintain processive DNA synthesis.

69 anslocating, which is an emerging feature of processive DNA unwinding enzymes.

70 Hexameric helicases are processive DNA unwinding machines but how they engage wi

71 t of the conserved domains, YonO is a highly processive DNA-dependent RNA polymerase.

72 We conclude that AdnAB's processive DSB unwinding activity suffices for AdnAB fun

73 These enzyme complexes are highly processive, duplex unwinding and degrading machines that

74 ate, leading to a dramatic increase in fast, processive dynein motility.

75 y and can synergize with dynactin to enhance processive dynein movement.

76 also increases the frequency and velocity of processive dynein movements that are activated by comple

77 offers insight into how cargo adaptors form processive dynein-dynactin motor complexes.

78 ein adaptors that promote assembly of highly processive dynein-dynactin motor complexes.

79 py to study the interaction of LIS1 with the processive dynein-dynactin-BicD2N (DDB) complex.

80 al polymerases, was found to be critical for processive elongation and virus growth.

81 ocessivity factor POT1-TPP1 fails to restore processive elongation in the presence of inhibitory dNTP

82 ng pockets of domain V and contributing to a processive elongation of alternan chains.

83 ly65Val allele in Li1 plants likely disrupts processive elongation of F-actin, resulting in a disorga

84 eins act as actin polymerases that drive the processive elongation of filament barbed ends in membran

85 Processive elongation of RNA Polymerase II from a proxim

86 roduct profiles displayed by Cps1B suggested processive elongation of the nascent polymer, whereas Cp

87 d subsequent intermediates leading to highly processive elongation, and we examined the effects of fo

88 e transition from distributive initiation to processive elongation.

89 om preferential substrate binding but during processive elongation.

90 Loop formation followed by processive enlargement provides a mechanism by which con

91 the discovery that the HECT E3 Nedd4-1 is a processive enzyme and that disruption of its processivit

92 ces cerevisiae Pol delta-PCNA is a rapid and processive enzyme, suggesting that CMG may bind and alte

93 CsaB is a distributive enzyme, and CsxA is a processive enzyme.

94 from Escherichia coli is a uniquely fast and processive enzyme.

95 terified zones, indicating that it acts as a processive enzyme.

96 que for measuring the structural kinetics of processive enzymes and other biomacromolecules.

97 Processive enzymes are major components of the efficient

98 Whereas processive enzymes bind only once to a substrate before

99 ave indicated that kinesin-14 motors are non-processive enzymes, working in the context of multi-moto

100 dicate that this organization helps to drive processive exo-ribonucleolytic cleavage.

101 RNase II, a 3' to 5' processive exoribonuclease, is the major hydrolytic enzy

102 llulose is produced by cellulose synthase, a processive family-2 glycosyltransferase.

103 This is the direct demonstration of a processive formin from plants.

104 transitions between the two complexes during processive genome packaging.

105 ng cell nuclear antigen and participation in processive genome replication are common features of FAM

106 uggest that B35DNAP may perform faithful and processive genome replication in vivo and, when required

107 heterodimeric co-factor A20.D4 required for processive genome synthesis.

108 obscured by a complex multistep mechanism of processive GHs.

109 Processive glycoside hydrolases (GHs), like cellobiohydr

110 Processive glycoside hydrolases are the key components o

111 se is synthesized by membrane-integrated and processive glycosyltransferases that polymerize UDP-acti

112 y electrophoresis shows that this PME is non-processive, halophilic, and acidophilic.

113 Kinesin-1, -2, -5, and -7 generate processive hand-over-hand 8-nm steps to transport intrac

114 The structure suggests a processive hand-over-hand unfolding mechanism, where eac

115 molecule approaches, we show that hDNA2 is a processive helicase capable of unwinding kilobases of ds

116 Our results indicate that PcrA is a highly processive helicase prone to stochastic pausing, resulti

117 stead as an adenosine triphosphate-dependent processive helicase when complexed with two accessory pr

118 an generate, leading to an apparently poorly processive helicase.

119 nd run length of a general model of finitely processive helicases, the two most commonly measured exp

120 ays revealed that Pol beta scans DNA using a processive hopping mechanism and has a mean search footp

121 is involved in base excision repair, uses a processive hopping search mechanism to locate substrates

122 description of how this channel facilitates processive hydrolysis of unbound cAMP.

123 core particles revealed that Pol beta is not processive in the context of a nucleosome, and its singl

124 PC-mediated ubiquitylation involves a highly processive initial reaction on the substrate, followed b

125 n of (5m)C by CD of mouse TET2 is chemically processive (iterative).

126 Mammalian KIF3AB is an N-terminal processive kinesin-2 that is best known for its roles in

127 KIF16B is a highly processive kinesin-3 family member that participates in

128 odimeric KIF17 and heterotrimeric KIF3AB are processive, kinesin-2 family motors that act jointly to

129 ficant reduction in the distances that these processive kinesins can travel.

130 ize, and a unified expression for predicting processive lifetime is revealed.

131 rocessive myosin ensembles and produce novel processive lifetime measurements.

132 s of prior experimental evidence that longer processive lifetimes are enabled by larger myosin ensemb

133 ion by Mre11-Rad50-Xrs2 and Sae2, as well as processive long-range resection by Sgs1-Dna2 or Exo1 pat

134 plate and synthesize RNA product in a highly processive manner in vitro.

135 able of liberating many sugar molecules in a processive manner without dissociating from the substrat

136 d full deacetylation of (GlcNAc)3-6 in a non-processive manner.

137 1D2), DDXs function in an ATP-dependent, non-processive manner.

138 P450 11B2 must produce aldosterone through a processive mechanism despite favoring a distributive mec

139 to 5hmC and 5fC formation arguing against a processive mechanism of 5mC oxidation.

140 kinetic model to further dissect Ena/VASP's processive mechanism on bundled filaments.

141 This so-called processive mechanism relies on a complex pattern of enzy

142 first evidence that DHX36 uses a local, non-processive mechanism to unwind G4 substrates, reminiscen

143 dy state kinetic model that accounts for the processive mechanism.

144 Intracellular transport is largely driven by processive microtubule- and actin-based molecular motors

145 tor that can work alone, to Eg5, a minimally processive mitotic motor that works in large ensembles.

146 maining mutations selectively compromise the processive mode of dynein movement that is activated by

147 hanges the reaction from a distributive to a processive mode.

148 Processive molecular motors enable cargo transportation

149 that unlike conventional myosin-II or other processive molecular motors, Ncd requires two ATP turnov

150 ects motor-microtubule complex stability and processive motility (the ability of an individual motor

151 Cargo selectivity and activation of processive motility depend on a group of so-called "acti

152 n autoinhibited conformation on its own, and processive motility is activated when it forms a ternary

153 Plus end-directed processive motility of KlpA on single microtubules depen

154 Here, we examined the processive motility of mammalian dynein complexed with d

155 ppears to be well matched to the duration of processive motility of the KIF16B motor, indicating the

156 lly exhibit long-distance minus-end-directed processive motility on single microtubules as individual

157 KlpA exhibits plus end-directed processive motility on single microtubules, but reverts

158 This processive motility requires gating mechanisms to coordi

159 ing of LIS1 to DDB does not strongly disrupt processive motility.

160 lly required for an allosteric activation of processive motility.

161 gy and significant motility, undergoing slow processive motion interrupted by short stretches of conf

162 Paradoxically, this resulting processive motion relies on quenching diffusive plasmid

163 ates that the origin of replication exhibits processive motion with a mean displacement that scales a

164 l of Cin8 is not necessary for bidirectional processive motion, but is controlling the switch between

165 the catalytic domain alone is sufficient for processive motion, providing insight into TrCel7A's mole

166 catastrophe inhibitor in vitro that uses its processive motor activity as part of a feedback loop to

167 ruct GiKIN14a-Deltatail is still a minimally processive motor and moves its center of mass via discre

168 tors, as well as with the requirement that a processive motor driving a large load via an elastic lin

169 shaped by evolution to cope with the lack of processive motor proteins in bacteria.

170 ess to explore the mechanochemical cycles of processive motor proteins such as kinesin-1, but it has

171 how that the mammalian kinesin-4 KIF21B is a processive motor that can accumulate at microtubule plus

172 he motor domain, KlpA is a plus end-directed processive motor; in contrast, when the tail detaches fr

173 nesin-3 family contains the fastest and most processive motors of the three neuronal transport kinesi

174 lity using assembling F-actin and two highly processive motors, myosin-5 and myosin-6.

175 Dimerization allows kinesins to be processive motors, taking many steps along the microtubu

176 Thus, processive motors, which commonly follow individual prot

177 a transport machine capable of long-distance processive movement along microtubules.

178 how that the yeast kinesin-14 Kar3 generates processive movement as a heterodimer with the non-motor

179 We observed that processive movement is rescued if ATP is added to the mu

180 The treadmilling dynamics direct the processive movement of the septal cell wall synthesis ma

181 ish the principles governing the dynamics of processive movement, we present a theoretical framework

182 to a ternary complex (termed DDB) capable of processive movement.

183 n to different membrane regions results from processive MreB motion in various geometries.

184 ize square complexes lead to defects in both processive MT plus-end tracking and polymerase.

185 The H3K4me3 mark promotes processive, multisite acetylation of histone H3 by Gcn5

186 neously binds a substrate-linked UB to drive processive multiubiquitination.

187 ational techniques are developed to simulate processive myosin ensembles and produce novel processive

188 sin motor head promotes highly selective and processive myosin V.

189 mechanochemical systems are investigated for processive myosin-actin motility systems.

190 er, much of our mechanistic understanding of processive myosins comes from in vitro studies in which

191 -dependent ADP release that is essential for processive myosins, such as myosin V.

192 Importantly, our study shows that processive Nedd4-1, but not the distributive Nedd4-1:inh

193 Here, we used the processive nuclease Cas3, together with a minimal Type I

194 Both human and yeast Exo1 are processive nucleases on their own.

195 corroborated by our observations that during processive nucleotide incorporation, sequential rounds o

196 During processive nucleotide incorporation, the first nucleotid

197 showed that human myosin Vc (MyoVc) was not processive on single-actin filaments [1-3].

198 ecords of motion reveal RNase R to be highly processive: one molecule can unwind over 500 bp of a str

199 We explored the possibility that MyoVc is processive only under conditions that resemble its biolo

200 val of phosphates can occur in a sequential (processive) or a stepwise (distributive) manner.

201 formation of the thioether bridges follows a processive order, providing mechanistic insights into ho

202 This compaction is processive over tens of kilobases at an average rate of

203 Because the latter monobody inhibits processive phosphorylation by Bcr-Abl and the SH2-kinase

204 Processive phosphorylation by SRPK stimulates RNF12-depe

205 TOG1 is critical for processive plus-end tracking, whereas TOG2 is critical f

206 In vitro, human full-length KIF1C is a processive, plus-end directed motor.

207 harp contrast to the correlated rotations of processive PMEs, and the substrate-binding groove is neg

208 ferent behavior and properties compared with processive PMEs.

209 gh-resolution cryo-EM structure of the human processive Pol delta-DNA-PCNA complex in the absence and

210 and unfurled TOG array states gives rise to processive polymerase activity at MT plus ends.

211 ication by varphi29 DNA polymerase, a highly processive polymerase enzyme that couples synthesis with

212 ophosphorolysis, providing new insights into processive polymerization.

213 ding to the backside of certain E2s promotes processive polyUb formation, but the mechanism remains e

214 s was ~8 s, consistent with these catalyzing processive positive supercoil relaxation in front of the

215 More importantly, during processive primer extension, pyrophosphate (PPi) release

216 hypothesized that actin cables regulate the processive properties of MyoVc.

217 This data supports for a processive, rather than distributed model of ERK phospho

218 otein splicing mediated by inteins is a self-processive reaction leading to the excision of the inter

219 Purified human telomerase catalyzes processive repeat synthesis, which could restore the ful

220 w model for DNA and RNA handling dynamics in processive repeat synthesis.

221 that this domain contributes to maintaining processive replication through G4-forming sequences, and

222 The chimeric polymerase carries out processive replication with PCNA in vitro; however, in y

223 , which mediate interactions with Pol31, and processive replication with the replication clamp prolif

224 erases depend on circular sliding clamps for processive replication.

225 ion during homologous recombination-supports processive resection by Exo1.

226 ssociates into smaller particles, and begins processive retrograde IFT in <2 s.

227 es positive for TrkB and BDNF, which exhibit processive retrograde motility with faster velocities th

228 ns but lack cytoplasmic dynein, the foremost processive retrograde transporter.

229 Processive, ring-shaped protein and nucleic acid protein

230 A combination of highly processive RNA polymerases, allosteric protein transcrip

231 Unlike their QDE-1(Ncr) counterpart favoring processive RNA synthesis, QDE-1(Tte) and QDE-1(Mth) prod

232 ter to transit into the elongation phase for processive RNA synthesis.

233 Although polymerization is efficient and processive, RNase H is inefficient and periodic.

234 ble for rod shape in Escherichia coli is the processive 'Rod complex'.

235 hen transmitted around the hexamer, reveal a processive rotary translocation mechanism and substrate-

236 structural complexity of long RNA molecules, processive RT enzymes are now required for preserving li

237 Notably, Ena/VASP's processive run length increases with the number of both

238 KIF3AC heterodimer modulates entry into the processive run regardless of whether the run is initiate

239 this study, the kinetics for entry into the processive run were quantified.

240 KIF3AB heterodimer modulates entry into the processive run, and the results suggest that it is the h

241 that either KIF3A or KIF3C can initiate the processive run.

242 cycles of the two motor heads to sustain the processive run.

243 uch that either KIF3A or KIF3C initiates the processive run.

244 inetics are similar for each head during the processive run.

245 A stochastic sequence of processive runs and diffusive searches results in a bias

246 ation of motility during degradation reveals processive runs and distinct steps on the scale of 1 nm.

247 ation selectively destroys the initiation of processive runs at physiological ATP levels.

248 a series of gating mechanisms, which lead to processive runs averaging ~1 mum.

249 hat Ena takes approximately threefold longer processive runs on trailing barbed ends of fascin-bundle

250 -1 function together to support fast, highly processive runs toward axon terminals.

251 gesting that the enzyme can initiate several processive runs without leaving the substrate.

252 Myo1c also regulates the termination of processive runs.

253 Application of our processive search assay into nucleosome core particles r

254 We developed a processive search assay to determine if Pol beta has evo

255 logous DNA Pol X family members to perform a processive search for 1-nucleotide gaps in DNA using a p

256 Of note, the differences in processive searching ability observed among the DNA Pol

257 Processive searching has been reported or proposed for m

258 nes within the lyase domain are required for processive searching, revealing a novel function for the

259 demonstrating both enzymes were deficient in processive searching.

260 otubule lattice structures, linking multiple processive segments together and contributing to KIF1A's

261 Importantly, the addition of Dna2 triggers processive Sgs1 translocation, but DNA resection only oc

262 litudes of contractions are tuned to achieve processive shrinking of cell surfaces remains unclear.

263 NusG-like proteins to lock RNAP in a closed, processive state during elongation.

264 cule results presented show that the kinesin processive stepping distance decreases 40-60% with EC50

265 d that DDB spends 65% of its time undergoing processive stepping, 4% undergoing 1D diffusion, and the

266 romotes a conformation of dynein optimal for processive stepping.

267 rotate around the ClpP apical surface during processive steps of translocation and proteolysis.

268 arlier computational analysis predicted that processive steps would alternate between a fast step for

269 mismatched ClpX and ClpP work in tandem for processive substrate translocation into the ClpP proteol

270 release, and it appears to limit the rate of processive synthesis at 4 degrees C.

271 alytic changes prevent potentially mutagenic processive synthesis by Rev1 and facilitate dissociation

272 Telomerase helps maintain telomeres by processive synthesis of telomere repeat DNA at their 3'-

273 ep, which appears to be rate-limiting during processive synthesis.

274 luding the rates of DNA translocation during processive synthesis.

275 ABE8e is more processive than ABE7.10, which could benefit screening,

276 ses to remove base methylations and a highly processive thermostable group II intron reverse transcri

277 that is able to unfold diverse substrates by processive threading through its central pore.

278 s or small molecules switches Nedd4-1 from a processive to a distributive mechanism of polyubiquitin

279 or of Nedd4-1, which switches Nedd4-1 from a processive to a distributive mechanism.

280 olymerase theta is frequently insufficiently processive to complete repair of breaks in microhomology

281 Spt5 forms a complex with Spt4 and regulates processive transcription elongation.

282 n human ribosomes during single-turnover and processive translation reactions.

283 hree tRNAs, which contributed to the rate of processive translation.

284 -induced DNA unwinding-including initiation, processive translocation, pausing and backtracking-and r

285 illustrated by comparing kinesin-1, a highly processive transport motor that can work alone, to Eg5,

286 ore how these dynamic structures can lead to processive transport of actin.

287 Although processive ubiquitination might-in principle-arise from

288 rate affinity on APC/C function, stimulating processive ubiquitylation of high-affinity substrates an

289 pecific disruption of Doc1/Apc10 function in processive ubiquitylation.

290 stabilize the active holoenzyme and promote processive ubiquitylation.

291 quitylation, initiation of translocation and processive unfolding of substrates, respectively.

292 By generalizing a previous model of processive unwinding by helicases, we provide a unified

293 on requires a RecQ dimer, and that continued processive unwinding of several kilobases involves multi

294 However, gp5 becomes highly processive upon formation of a complex with Escherichia

295 for RNA binding, subunit oligomerization and processive UTP incorporation, and predict druggable pock

296 rm tetramers and provide an amended model of processive VASP-mediated actin assembly in clustered arr

297 limiting reaction regimes, distributive and processive, which differ in the number of enzyme-substra

298 sequence for coiled-coil formation is highly processive with run lengths of approximately 1.23 mum an

299 neck linker and helix alpha7 is also highly processive with run lengths of approximately 1.62 mum an

300 s translocation activity is rapid and highly processive, with individual complexes traveling an avera