ライフサイエンスコーパス: prolyl 4-hydroxylase

1 uding Lh2b (lysyl oxidase-like 2b) and P4ha (prolyl 4-hydroxylase).

2 substrate for the collagen-modifying enzyme prolyl 4-hydroxylase.

3 -2, that encode alpha subunits of the enzyme prolyl 4-hydroxylase.

4 olding and assembly is distinct from that of prolyl 4-hydroxylase.

5 1026_II1055 (bcaB), which encodes a putative prolyl 4-hydroxylase.

6 EGLN1, hypoxia-inducible gene 1 (HIG1), and prolyl 4-hydroxylase.

7 o human PHD2 and a Chlamydomonas reinhardtii prolyl-4-hydroxylase.

8 TET 5-methylcytosine hydroxylases, and EglN prolyl-4-hydroxylases.

9 ind to protein disulfide isomerase (PDI) and prolyl 4-hydroxylase 1 (P4H1).

10 Prolyl 4-hydroxylase-1 (P4H1) was previously implicated

11 e2Cre-mediated disruption of Egln1 (encoding prolyl-4 hydroxylase 2 [PHD2]; Egln1(Tie2)) in endotheli

12 encoding for hypoxia-inducible factor (HIF) prolyl-4-hydroxylase 2 (PHD2), cause erythrocytosis and

13 ing RNA (siRNA) to silence murine HIF-1alpha-prolyl-4 hydroxylase-2 (PHD2).

14 c oxide synthase (eNOS), Akt, and HIF-1alpha-prolyl-4-hydroxylase-2 (PHD)2 expression were measured.

15 For a third system, viral prolyl 4-hydroxylase (26 kDa), ECD showed that multiple

16 colocalization of antibodies to PGP 9.5 and prolyl-4-hydroxylase (a fibroblast marker) as well as co

17 PDI-2 is required for the normal function of prolyl 4-hydroxylase, a key collagen-modifying enzyme.

18 ated deletions within each gene to eliminate prolyl 4-hydroxylase activity from the animal.

19 toglutarate/lactate ratio, likely inhibiting prolyl-4-hydroxylase activity.

20 1 target genes and discovered that the phy-2 prolyl 4-hydroxylase alpha subunit is critical for survi

21 peptide that acts as the beta-subunit in the prolyl 4-hydroxylase alpha(2)beta(2)-tetramer (P4H) and

22 ading enzymes, and decreased LOX, LOXL2, and prolyl 4-hydroxylase alpha-1 (P4HA1) for ECM processing

23 K catalytic subunit (DNA-PKcs), HA95, Hsp27, prolyl 4-hydroxylase alpha-1 subunit, alpha-tubulin, and

24 nuclear YAP1 accumulation and expression of prolyl 4-hydroxylase alpha-2 (P4HA2) which increases col

25 ted that these 3 genes are apolipoprotein E, prolyl 4-hydroxylase alpha-subunit, and an unknown trans

26 physical map, and we show that it encodes a prolyl-4-hydroxylase alpha catalytic subunit.

27 Prolyl-4-hydroxylase alpha(I) (P4Halpha(I)) is the rate-

28 riptionally activates the alpha(II) collagen prolyl-4-hydroxylase [alpha(II)PH] gene, resulting in th

29 D44(+), SH2(+), SH3(+), and SH4(+); produced prolyl-4-hydroxylase, alpha-smooth muscle actin, fibrone

30 n, along with the alpha and beta subunits of prolyl 4-hydroxylase, an enzyme that modifies the collag

31 elopment and is mediated by a HIF-alpha type prolyl 4-hydroxylase and five sequentially acting cytopl

32 erved residues present in the active site of prolyl 4-hydroxylase and lysyl hydroxylase.

33 nase domains similar to the alpha-subunit of prolyl 4-hydroxylase and lysyl hydroxylases.

34 amily of O2-dependent HIF hydroxylases: four prolyl 4-hydroxylases and an asparaginyl hydroxylase.

35 Since prolyl-4-hydroxylase and protein disulfide isomerase coe

36 y collagen prolyl 3-hydroxylase and collagen prolyl 4-hydroxylase, and is essential for normal cell f

37 enes, (5) other HRGPs, glycosyl transferase, prolyl 4-hydroxylase, and peroxidase genes coexpressed w

38 Hypoxia-inducible factor (HIF) prolyl 4-hydroxylases are a family of iron- and 2-oxoglu

39 w molecular weight inhibitors of procollagen prolyl 4-hydroxylase as potential inhibitors of the HIF

40 , we identified carbonyl modification of the prolyl-4-hydroxylase beta subunit (P4Hb) that is respons

41 D application, but there was an increase in prolyl-4-hydroxylase-beta expression, indicative of incr

42 oliferation, apoptosis, satellite cells, and prolyl-4-hydroxylase-beta expression.

43 an enzymatic activity distinct from that of prolyl 4-hydroxylase, but no amino acid sequences or gen

44 Collagen prolyl 4-hydroxylases (C-P4H) are alpha(2)beta(2) tetram

45 Collagen prolyl 4-hydroxylases (C-P4H) are required for formation

46 Collagen prolyl 4-hydroxylases (C-P4H-I, C-P4H-II, and C-P4H-III)

47 Collagen prolyl 4-hydroxylases (C-P4Hs) are key enzymes in collag

48 Collagen prolyl 4-hydroxylases (C-P4Hs) play a central role in th

49 a-(P4H-beta) subunits of the type I collagen prolyl-4-hydroxylase (C-P4H(I)).

50 show that hypoxia increases type I collagen prolyl-4-hydroxylase [C-P4H(I)], which leads to prolyl-h

51 synthesis at a post-translational level in a prolyl 4-hydroxylase-dependent manner that does not requ

52 in the kidney and liver and is regulated by prolyl-4-hydroxylase domain (PHD) dioxygenases PHD1, PHD

53 iminates between the inhibition of HIF-alpha prolyl-4-hydroxylase domain (PHD) enzymes and HIF-alpha

54 The prolyl-4-hydroxylase domain (PHD) enzymes are regarded a

55 F-1alpha in these cells is controlled by the prolyl-4-hydroxylase domain (PHD) family of proteins.

56 ulated in response to oxygen availability by prolyl-4-hydroxylase domain (PHD) proteins, with PHD2 be

57 e targeting to examine the von Hippel-Lindau/prolyl-4-hydroxylase domain (PHD)/HIF axis in cell-expre

58 Transfections with plasmids coding prolyl-4-hydroxylase domain protein 2 (PHD2) and ShPHD2

59 Prolyl-4-hydroxylase domain-containing proteins 1-3 (PHD

60 Prolyl-4-hydroxylase-domain (PHD) proteins regulate the

61 collagen is post-translationally modified by prolyl-4-hydroxylase (EC 1.14.11.2) before secretion and

62 f each of the hypoxia inducible factor (HIF) prolyl-4-hydroxylase enzymes (PHD1-3) in the first 24 h

63 e collagen prolyl 3-hydroxylase and collagen prolyl 4-hydroxylase families as potentially important r

64 e collagen prolyl 3-hydroxylase and collagen prolyl 4-hydroxylase families in melanoma.

65 P4HA3 in a subset of melanomas, the collagen prolyl 4-hydroxylase family members P4HA1, P4HA2, and P4

66 n of the fibroblast markers type I collagen, prolyl-4-hydroxylase, fibroblast specific protein-1, and

67 rt for the animal-derived O2-sensor model of prolyl 4-hydroxylase function, in an organism that lacks

68 Co-transfection with prolyl 4-hydroxylase generated homotrimers with stable p

69 hat expression of hypoxia-regulated collagen prolyl-4-hydroxylase genes P4HA1 and P4HA2 is significan

70 Although prolyl 4-hydroxylase has been characterized as an alpha2

71 While prolyl-4-hydroxylase has been studied extensively by bio

72 Hypoxia-inducible factor (HIF) prolyl 4-hydroxylases (HIF-P4Hs 1-3) are druggable targe

73 Hypoxia-inducible factor prolyl 4-hydroxylases (HIF-P4Hs) regulate the hypoxic in

74 As oxygen sensors of the cells, the HIF prolyl 4-hydroxylases (HIF-P4Hs) regulate the stability

75 gen VIII molecules expressed with or without prolyl 4-hydroxylase identified urea-sensitive high mole

76 t body morphology, consistent with a role of prolyl 4-hydroxylase in formation of the body cuticle.

77 us solution, azaquinolone inhibitors bind to prolyl 4-hydroxylase in two different orientations, as f

78 s the first report of a mutationally defined prolyl-4-hydroxylase in any animal.

79 e midleaflet and free edge and expression of prolyl-4-hydroxylase (indicating collagen synthesis) was

80 ells demonstrated expression of vimentin and prolyl-4-hydroxylase, indicating a mesenchymal phenotype

81 that prevention of fibrosis after MI with a prolyl 4-hydroxylase inhibitor (P4HI) would preserve LV

82 Addition of the prolyl 4-hydroxylase inhibitor 2,2-dipyridyl, however, e

83 Cs that were cultured in the presence of the prolyl-4-hydroxylase inhibitor dimethyloxalylglycine (DM

84 factor (HIF) pathway under normoxia using a prolyl-4-hydroxylase inhibitor, dimethyloxalylglycine (D

85 ntified low molecular weight and peptide HIF prolyl 4-hydroxylase inhibitors as novel neurological th

86 We also showed that structurally diverse HIF prolyl 4-hydroxylase inhibitors prevent oxidative death

87 inhibition with multiple selective collagen prolyl 4-hydroxylase inhibitors reduces proliferation an

88 Prolyl 4-hydroxylases install a hydroxyl group in the 4R

89 uggest that selective inhibition of collagen prolyl 4-hydroxylase is an attractive strategy to reduce

90 own of P4ha1 and/or P4ha2, encoding collagen prolyl 4-hydroxylase isoenzymes.

91 , by inhibiting the hypoxia-inducible factor prolyl 4-hydroxylase isoform 1 (PHD1), an iron and 2-oxo

92 thylases, TET DNA demethylases, and collagen prolyl 4-hydroxylases, leading to accumulation of methyl

93 Ofd1, a prolyl 4-hydroxylase-like 2-oxoglutarate dioxygenase, co

94 Ofd1, an uncharacterized prolyl 4-hydroxylase-like 2-oxoglutarate-Fe(II) dioxygen

95 (II)-dependent protein hydroxylases, such as prolyl 4-hydroxylase or lysyl hydroxylases.

96 Soluble cytosolic extracts have Skp1 prolyl 4-hydroxylase (P4H) activity, which can be measur

97 aperone and being a component of the enzymes prolyl 4-hydroxylase (P4H) and microsomal triglyceride t

98 Prolyl 4-hydroxylase (P4H) catalyzes the posttranslation

99 Prolyl 4-hydroxylase (P4H) is a nonheme iron dioxygenase

100 In a screen of prolyl 4-hydroxylase (P4H) mutants, we found that geneti

101 l hydroxylation (CPH), which is catalyzed by prolyl 4-hydroxylase (P4H), is the most prevalent posttr

102 An endoplasmic reticulum transmembrane prolyl 4-hydroxylase (P4H-TM) is able to hydroxylate the

103 A transmembrane prolyl 4-hydroxylase (P4H-TM) is highly expressed in the

104 ndoplasmic reticulum-localized transmembrane prolyl 4-hydroxylase (P4H-TM)-is found in animals.

105 Prolyl 4-hydroxylases (P4H) catalyze the post-translatio

106 Prolyl-4-hydroxylase (P4H) is a non-heme iron hydroxylas

107 two transient complexes in the reaction of a prolyl-4-hydroxylase (P4H), a functional homologue of hu

108 ium expresses, in all cells, an O2-dependent prolyl 4-hydroxylase (P4H1) required for O-glycosylation

109 Dictyostelium expresses an HIFalpha-type prolyl 4-hydroxylase (P4H1) whose levels affect the O(2)

110 Proteomic profiling identified the prolyl 4-hydroxylase (P4HB, also known as PDIA1) as a ma

111 Prolyl 4-hydroxylases (P4Hs) are mononuclear non-heme ir

112 Prolyl 4-hydroxylases (P4Hs) catalyze post-translational

113 Specific prolyl 4-hydroxylases (P4Hs) regulate the stability of t

114 nal modification (PTM) of HRGPs catalyzed by prolyl 4-hydroxylases (P4Hs), defines their subsequent O

115 h glycoproteins (HRGPs) that is catalyzed by prolyl 4-hydroxylases (P4Hs).

116 els of HIF-1alpha transcripts, low levels of prolyl-4-hydroxylases (PHD2 and PHD3), and a low cellula

117 ne such environmental factor and cytoplasmic prolyl 4-hydroxylases (PHDs) are evolutionarily conserve

118 Cytoplasmic prolyl 4-hydroxylases (PHDs) have a primary role in O(2)

119 PHD1 belongs to the family of prolyl-4-hydroxylases (PHDs) that is responsible for pos

120 ma gondii is hydroxylated by an O2-dependent prolyl-4-hydroxylase (PhyA), and the resulting hydroxypr

121 g F-box and WD-40 domain protein (Fbxw7) and Prolyl 4-hydroxylase possessing a transmembrane domain (

122 Previous studies showed that a prolyl 4-hydroxylase related to animal HIFalpha prolyl h

123 stelium lacks HIFalpha, a mediator of animal prolyl 4-hydroxylase signaling, and P4H1 can hydroxylate

124 ular weight or peptide inhibitors of the HIF prolyl 4-hydroxylases stabilize HIF-1alpha and up-regula

125 sed levels of the miRNA biogenesis molecules prolyl 4-hydroxylase subunit alpha 1 (P4HA1) and Ago2 al

126 s in mitochondrial metabolism as elicited by prolyl 4-hydroxylase subunit alpha 1 (P4HA1) to T cell e

127 odel wherein conditional genetic ablation of prolyl 4-hydroxylase subunit beta (P4HB), the gene that

128 not viable, suggesting an essential role for prolyl 4-hydroxylase that is normally accomplished by ei

129 However, unlike the prolyl 4-hydroxylases that regulate mammalian hypoxia-in

130 , cellular hypoxic responses are mediated by prolyl 4-hydroxylases that use O(2) and alpha-ketoglutar

131 d PHD3, oxygen- and 2-oxoglutarate-dependent prolyl-4-hydroxylases that regulate HIF activity.

132 The ability of the inhibitors of HIF prolyl 4-hydroxylases to stabilize proteins involved in

133 ve consanguineous families in P4HTM encoding prolyl 4-hydroxylase transmembrane (P4H-TM).

134 ere mimicked by small-molecule inhibitors of prolyl 4-hydroxylase, validating the genetic results and

135 HIF-1 activation is controlled by HIF-1alpha-prolyl 4-hydroxylases, which target HIF-1alpha for ubiqu

136 ed by examining this required interaction of prolyl-4-hydroxylase with procollagen.