ライフサイエンスコーパス: pronephric

1 In addition, elevated Notch signaling in the pronephric anlage both perturbed the characteristic patt

2 bition was the result of a failure to form a pronephric anlage of appropriate size rather than a defe

3 lops from the ventroposterior portion of the pronephric anlage, is missing from more of the mild UV p

4 potential binding partners in both dissected pronephric anlagen and in individual dissected component

5 nd that during tubulogenesis, the developing pronephric anlagen expresses Daam1 and its interacting R

6 Wnt-4 then patterns the proximal pronephric anlagen to establish the specific compartment

7 a Wnt-4 function that patterns the proximal pronephric anlagen.

8 3 d, hPSCs are induced into PAX2(+)GATA3(+) pronephric (anterior) intermediate mesoderm fates in mon

9 PKD2 suppression inactivates CaMK-II in pronephric cells and cilia, whereas constitutively activ

10 nsporter expression is lost, indicating that pronephric cells are transfated to MCCs.

11 fewer endothelial cells as well as far more pronephric cells compared to wildtype.

12 retain the normal number of endothelial and pronephric cells.

13 flr pronephric cilia were shortened and showed a reduced bea

14 s a role for lmx1b in the development of the pronephric components.

15 enesis, and expansion of MCCs occurred after pronephric cyst formation and was inversely correlated w

16 n of cilia structure or motility resulted in pronephric cyst formation, hydrocephalus and left-right

17 on in sentinel fish results in a synergistic pronephric cyst phenotype, revealing a genetic interacti

18 lialization/polarity in renal cells and with pronephric cysts and microphthalmia in zebrafish embryos

19 rafish CC2D2A ortholog (sentinel) results in pronephric cysts, a hallmark of ciliary dysfunction anal

20 zebrafish models of JBTS (curved body shape, pronephric cysts, and cerebellar abnormalities) and redu

21 including axis curvature, hydrocephalus, and pronephric cysts, and disrupts multicilia motility, sugg

22 partially rescued pkd2 morphant phenotypes, pronephric cysts, hydrocephalus, and body curvature.

23 ranslation-blocking morpholinos (MOs) caused pronephric cysts, hydrocephalus, and body curvature.

24 ronal architecture and with the formation of pronephric cysts, respectively.

25 Knockdown of fat1 in zebrafish causes pronephric cysts, which is partially rescued by RAC1/CDC

26 rtholog of Nek8 resulted in the formation of pronephric cysts.

27 ahorse, a zebrafish mutation that results in pronephric cysts.

28 ckdown of miR-30a-5p phenocopied most of the pronephric defects observed upon global inhibition of mi

29 s was substantiated in Xenopus, in which the pronephric defects of bicc1 knockdowns were rescued by r

30 kdown of greb1l in zebrafish caused specific pronephric defects, which were rescued by wild-type huma

31 suggest that a medial signal is required for pronephric development and show that the signal is propa

32 time dependent manner and was essential for pronephric development in Xenopus embryos.

33 equirement for pax2.1 in multiple aspects of pronephric development including tubule and duct epithel

34 ndicates that it normally functions later in pronephric development than does XPax-8.

35 (4) Throughout pronephric development the interglomerular mesangial cel

36 hese studies reveal the similarity of normal pronephric development to kidney organogenesis in all ve

37 d a requirement for Notch signaling early in pronephric development, before the pattern of MCC differ

38 tubule specification and persists throughout pronephric development.

39 kdown of Prdm15 in Xenopus embryos disrupted pronephric development.

40 restricting hemato-vascular fate in favor of pronephric development.

41 ration, generalized edema, glomerular cysts, pronephric dilatation, and expansion of kidney cilia.

42 ystic kidney diseases (CKD) in humans, cause pronephric dilations in zebrafish.

43 zebrafish embryos, bnc2 was expressed in the pronephric duct and cloaca, analogs of the mammalian low

44 on of vasculogenesis and concomitant loss of pronephric duct and somitic tissue.

45 alpha 6 is also expressed in the elongating pronephric duct as well as a subset of the rhombencephal

46 topic podocyte-specific marker expression in pronephric duct cells correlates with loss of expression

47 ufficient to direct pathfinding of migrating pronephric duct cells in axolotl embryos by: (1) demonst

48 kidney development with highly disorganized pronephric duct cilia.

49 asolateral membrane protein targeting in the pronephric duct epithelial cells is also severely affect

50 These include pronephric duct extension, "gill bulge" formation, and e

51 ereas constitutively active CaMK-II restores pronephric duct formation in pkd2 morphants.

52 are incorporated into an integrated model of pronephric duct guidance consistent with all present evi

53 The role of this matrix in pronephric duct guidance was assayed by presenting matri

54 e basal bodies in multiciliated cells of the pronephric duct in ift mutants were disorganized, with a

55 he epidermis overlying the migrating axolotl pronephric duct is known to participate in duct guidance

56 We have previously shown that pronephric duct is specified somewhat later, at stage 14

57 ds its effect to head, heart, pronephros and pronephric duct mesoderm inducing early blood and endoth

58 ucts caused a corresponding reorientation of pronephric duct migration.

59 pidermally deposited matrix from reorienting pronephric duct migration.

60 were detected and localized here, inhibited pronephric duct migration.

61 ies that recognize the pronephric tubules or pronephric duct to explore the induction of the embryoni

62 tion occurs after the differentiation of the pronephric duct with both the glomeruli and tubules bein

63 Interestingly the pronephric duct, which develops from the ventroposterior

64 also required for ciliogenesis in zebrafish pronephric duct.

65 hpf is confined to the lens, cerebellum, and pronephric duct.

66 ective zebrafish had too few motile cilia in pronephric ducts and in Kupffer's vesicle.

67 and disrupted ciliogenesis in the developing pronephric ducts and otic vesicles.

68 sited on microcarriers directly to migrating pronephric ducts in situ.

69 s, which give rise to the inner ear; and the pronephric ducts of the kidney.

70 -II-deficient zebrafish embryos fail to form pronephric ducts properly, and exhibit anterior cysts an

71 tion of F-actin and Na(+)/K(+)-ATPase in the pronephric ducts was disturbed.

72 lacodes with defective morphology as well as pronephric ducts with increased polyglutamylation.

73 ecreased ciliary length in the spinal canal, pronephric ducts, and Kupffer's vesicle.

74 ral tube, retina, notochord, somites, heart, pronephric ducts, branchial arches, and jaw muscles in e

75 efects of the pronephric tubules but not the pronephric ducts, consistent with the tubular atrophy ob

76 s such as the brain, olfactory placodes, and pronephric ducts.

77 otic vesicle and shorter motile cilia in the pronephric ducts.

78 bules, whereas zSMCTe mRNA is more distal in pronephric ducts.

79 ssed in MCCs and that notch3 is expressed in pronephric epithelial cells.

80 Daam1 resulted in reduced expression of late pronephric epithelial markers with no apparent effect up

81 absent in trunk IM, although endothelial and pronephric expression is retained, suggesting that early

82 We also identified pronephric expression of lunatic fringe and radical frin

83 ric structures on both sides, but the caudal pronephric extension was attenuated on the cut side.

84 Fgf signaling we show that Fgfs both promote pronephric fate and repress blood and endothelial fate.

85 intermediate mesoderm as candidates for the pronephric field by expression patterns of the Wilms' Tu

86 The most anterior cells in the pronephric field give rise to podocytes.

87 As is true for other organ fields, the pronephric field regulates after focal destruction, such

88 duct are restricted to subdomains within the pronephric field.

89 These techniques, assessing pronephric function, highlight the potential for in vivo

90 In addition to pronephric function, ponzr1 is required for pharyngeal a

91 esting a defect associated with the onset of pronephric function.

92 nes scl and gata1 was also observed, whereas pronephric gene pax2a was relatively normal in expressio

93 umulation, delayed development, and signs of pronephric glomerular and tubular dysfunction mimicking

94 Pronephric glomerular midline fusion was compromised in

95 , we focus on the morphogenetic movements of pronephric glomerular primordia (PGP) occurring during z

96 (3) In zebrafish, the bilateral pair of pronephric glomeruli is fused at the midline to form a g

97 The morphological processes forming the pronephric glomerulus are astoundingly different between

98 ts of using teleost models to understand the pronephric glomerulus development.

99 rates that in fish, the morphogenesis of the pronephric glomerulus is not stereotypical.

100 In zebrafish, the pronephric glomerulus occupies a midline position undern

101 LIM homeodomain protein, is expressed in the pronephric glomus.

102 xplant studies revealed that the prospective pronephric IM is already specified to express kidney gen

103 ed embryos are still competent to respond to pronephric-inductive signals.

104 tudy reconstructs the cellular makeup of the pronephric kidney and identifies conserved cells, segmen

105 )(+) that both promotes morphogenesis of the pronephric kidney and stabilizes primary cloacal cilia.

106 rafish embryos caused ciliary defects in the pronephric kidney at 27 h postfertilization and distensi

107 of Polycystin-2 mRNA expression resulted in pronephric kidney cysts, body axis curvature, organ late

108 To study the role of miRNAs during pronephric kidney development of Xenopus, global miRNA b

109 , such as curly tail up, edema, and abnormal pronephric kidney development.

110 bty mRNA was expressed by the pronephric kidney of N. coriiceps at a steady-state leve

111 Using the pronephric kidney of Xenopus laevis we discovered that t

112 n and amphibian nephrons, the Xenopus laevis pronephric kidney offers a simplified model for studying

113 The zebrafish pronephric kidney provides a simplified model of nephron

114 understanding of the cellular makeup of the pronephric kidney remains incomplete.

115 he complex phenotype of cholera toxin in the pronephric kidney was caused by the hyperactivation of a

116 Indeed, the cells in the Xenopus pronephric kidney were positive for the lipid raft marke

117 Here, we took advantage of the zebrafish pronephric kidney which forms directly from intermediate

118 Thus, there is a field for the pronephric kidney with classical attributes of defined b

119 cluding curly tail and cyst formation in the pronephric kidney, caused by down-regulation of endogeno

120 ed truncated expression of megalin along the pronephric kidney, consistent with a shortened S1 segmen

121 Due to their fully functioning pronephric kidney, larval zebrafish have become a popula

122 omus, the filtration device of the amphibian pronephric kidney, using an explant culturing strategy i

123 n Xenopus resulted in a dramatically smaller pronephric kidney.

124 differentiated epithelial structures of the pronephric kidney.

125 pholino oligonucleotide to pkd2 in zebrafish pronephric kidney.

126 in order to efficiently direct cells to form pronephric kidneys, XPax-8 requires cofactors, one of wh

127 ile restricting the expression domain of the pronephric marker pax2.1.

128 Pronephric markers were strongly expressed caudal to the

129 Explants of presumptive pronephric mesoderm were dissected from embryos of mid-g

130 the loss of tissues capable of inducing the pronephric mesoderm, as marginal zone explants from vent

131 development, however, is expanded only into pronephric mesoderm, remaining excluded from head, heart

132 h potential binding partners can up-regulate pronephric molecular markers suggesting that lmx1b lies

133 tamylation in fleer/ift70 mutants and rescue pronephric multicilia formation in both fleer- and ift88

134 ll mRNA sequencing of the functional Xenopus pronephric nephron and evaluated its cellular compositio

135 cell membranes, and occlusion of the caudal pronephric nephron segment.

136 mutation studies in zebrafish revealed that pronephric nephrons require osr1 for proximal tubule and

137 Our results indicate that early phase pronephric Notch signalling patterns the medio-lateral a

138 the former experiments, not the migration of pronephric or mesonephric precursor cells from the primi

139 mental knockdown of Bnc2 in zebrafish caused pronephric-outlet obstruction and cloacal dilatation, ph

140 f either gene alone has a moderate effect on pronephric patterning, while coexpression of XPax-8 plus

141 e that, ctns gene is essential for zebrafish pronephric podocyte and proximal tubular function and th

142 odocytes and required for the development of pronephric podocyte cell structure.

143 osr1-deficient pronephric podocyte progenitors express the Wilms' tumor

144 n and podocin were specifically expressed in pronephric podocytes and required for the development of

145 increased the expression of early markers of pronephric precursor cells, Pax-2 and Wilms' tumor suppr

146 og) in the gut endoderm, pax2 and wt1 in the pronephric primordial, and valentino (val) in the hindbr

147 terning defect during differentiation of the pronephric primordium and that mutually inhibitory regul

148 ax2.1 expression in the lateral cells of the pronephric primordium is required to restrict the domain

149 a key early step in the establishment of the pronephric primordium.

150 The hPSCs are first induced into pronephric progenitor cells at 90% efficiency and then a

151 n of the intermediate mesoderm that provides pronephric progenitors.

152 h markers of haematopoietic, endothelial and pronephric progenitors.

153 y unappreciated roles for sim1a in zebrafish pronephric proximal tubule and CS patterning, and are co

154 ateral cell membranes, whereas in the caudal pronephric segment, Polycystin-2 was concentrated in sub

155 indicated that injected xWT1 mRNA inhibited pronephric specification prior to any overt sign of morp

156 m-1 expression, an early molecular marker of pronephric specification, in tailbud embryos indicated t

157 Embryos manipulated after stage 9 developed pronephric structures on both sides, but the caudal pron

158 chord and the trunk paraxial mesoderm formed pronephric structures on both sides, regardless of the s

159 rsion of chick intermediate mesoderm (IM) to pronephric tissue and Lmx-1 mRNA expression as a marker

160 ) and, as we demonstrate, can induce ectopic pronephric tissue in Xenopus ectodermal organoids.

161 ls correlates with loss of expression of the pronephric tubule and duct-specific markers mAb 3G8 and

162 All have visible cysts in place of the pronephric tubule at 2-2.5 days of development.

163 Obstruction of the zebrafish pronephric tubule caused a rapid increase in cilia beat

164 diate mesoderm to test six2b function during pronephric tubule development and differentiation.

165 ern of Xanx-4 suggests it may have a role in pronephric tubule development.

166 bryos by mRNA injection and found to inhibit pronephric tubule development.

167 The results suggest that the failure in pronephric tubule differentiation in noi arises from a p

168 six2b morphants and mutants showed disrupted pronephric tubule morphogenesis.

169 The enlarged pronephric tubule phenotype observed may be attributed t

170 transcription is upregulated at the time of pronephric tubule specification and persists throughout

171 two nephrons with fused glomeruli and paired pronephric tubules and ducts.

172 l-C developed generalized edemas and dilated pronephric tubules and ducts.

173 We find that the pronephric tubules are present in all but the strongest

174 ified at stage 12.5, the same stage at which pronephric tubules are specified.

175 h embryos induced convolution defects of the pronephric tubules but not the pronephric ducts, consist

176 It has been shown that pronephric tubules can be induced to form in presumptive

177 ole for annexin IV in the development of the pronephric tubules in Xenopus laevis.

178 sed monoclonal antibodies that recognize the pronephric tubules or pronephric duct to explore the ind

179 usly undescribed migration pathway along the pronephric tubules to initiate adult hematopoiesis in th

180 Pronephric tubules were also shown to be reduced in stru

181 strates that noi(- )larvae specifically lack pronephric tubules while glomerular cell differentiation

182 s are specifically located to the developing pronephric tubules, and the protein to the luminal surfa

183 Unlike the pronephric tubules, the glomus can also be induced by FG

184 thin the kidney, zSMCTn mRNA is expressed in pronephric tubules, whereas zSMCTe mRNA is more distal i

185 from more of the mild UV phenotypes than are pronephric tubules.

186 and also leads to the development of ectopic pronephric tubules.

187 a normal glomerulus and cysts limited to the pronephric tubules.

188 These data indicate that pronephric tubulogenesis requires the Daam1/WGEF/Rho PCP

189 aam1 signaling pathway significantly reduced pronephric tubulogenesis.