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1  oocyte cortex and eccentric para-tangential pronuclear alignment compared to IVF zygotes.
2 downstream target of the APC, causes similar pronuclear and a-p polarity defects.
3                                        Here, pronuclear and mitotic events in nonhuman primate zygote
4 In this issue of Developmental Cell, using a pronuclear assembly assay, Xue et al. demonstrate that D
5 nopus egg extracts excess microtubules cause pronuclear assembly defects, leading to abnormal morphol
6 f the maternal pronucleus and argues against pronuclear asynchrony as the primary cause of CI lethali
7                              This stimulated pronuclear centering, DNA synthesis, and nuclear envelop
8 pronuclear migration, and moreover, a robust pronuclear centration and rotation very similar to that
9 e spatial organization and forces needed for pronuclear centration, rotation, and spindle displacemen
10 itially by the migration and rotation of the pronuclear complex (PNC) and its two associated astral m
11 tween initial centration and rotation of the pronuclear complex and entry into mitosis, and the model
12  simultaneous centration and rotation of the pronuclear complex observed in vivo.
13 ential for nucleus-centrosome attachment and pronuclear congression during fertilization.
14 e, but macroH2A is progressively lost during pronuclear decondensation prior to synkaryogamy.
15 itment and degradation of maternal mRNA, and pronuclear development.
16 in and terminate development after the first pronuclear division.
17 on, and centrosome association with the male pronuclear envelope are defective in dli-1(RNAi) embryos
18 showed that this event is required to form a pronuclear envelope scission event that removes membrane
19 he recruitment of YA or lamin Dm to the male pronuclear envelope, suggesting that the mutations affec
20 vity are incompatible with the presence of a pronuclear envelope.
21 s appear incompatible with the presence of a pronuclear envelope.
22 s MAP kinase remains active, however, and no pronuclear envelopes form.
23 membranes on the juxtaposed oocyte and sperm pronuclear envelopes in the zygote, allowing the parenta
24 n and mitotic spindle elongation facilitates pronuclear envelopes scission and parental genomes unifi
25 cal microtubule pulling forces contribute to pronuclear envelopes scission by promoting mitotic spind
26       We also demonstrate that weakening the pronuclear envelopes via PLK-1-mediated lamina depolymer
27 ly with the formation of the male and female pronuclear envelopes.
28 (anchoring nuclei to the exchange junction), pronuclear exchange, pronuclear fusion, and anchoring po
29 hybrids: (1) oocyte control of sperm-derived pronuclear expansion and (2) polar body formation.
30                                   When sperm pronuclear expansion is delayed, centrosomes detach, lea
31  Whereas microtubules normally promote sperm pronuclear expansion, in Dppa2-depleted Xenopus egg extr
32  the action potential, sperm entry, and male pronuclear formation can occur in the absence of PLCgamm
33  that PAWP triggers calcium oscillations and pronuclear formation in human and mouse oocytes similar
34 a2+ action potential, sperm entry, and sperm pronuclear formation were not prevented by injection of
35 ulum, an increase in intracellular pH, sperm pronuclear formation, MAP kinase dephosphorylation, and
36 t a precise time and location during nascent pronuclear formation.
37 dc2/cyclin B1 and MAP kinase activities, and pronuclear formation.
38 iated with nuclear structures at the time of pronuclear formation.
39 imately 4,000 genes are transcribed prior to pronuclear fusion and in the one- to four-cell embryos.
40 8 and Y11, inhibitors of FAK, interfere with pronuclear fusion and reduce the abundance of pY(397)FAK
41 emonstrate that Brambleberry is required for pronuclear fusion following fertilization in zebrafish.
42 immediately after fertilization and prior to pronuclear fusion in metazoa, suggesting that newly tran
43 the exchange junction), pronuclear exchange, pronuclear fusion, and anchoring postzygotic nuclear div
44 diately after fertilization in utero, before pronuclear fusion, and before the first cleavage of the
45  decondensation of the sperm chromatin after pronuclear fusion, nuclear envelope breakdown and format
46                                           At pronuclear fusion, the diploid zygotic nucleus was assoc
47 y transcribed in the A. suum zygote prior to pronuclear fusion.
48 a known defect of klp3A oocytes in mediating pronuclear fusion.
49 development, we generated transgenic mice by pronuclear injection of a 380 kb yeast artificial chromo
50                 These mice were generated by pronuclear injection of a bacterial artificial chromosom
51 ng-free method to target the mouse genome by pronuclear injection of a commercial Cas9 protein:crRNA:
52            Transgenic mice were generated by pronuclear injection of a construct carrying a 3-kilobas
53 l for Charcot-Marie-Tooth disease type 1A by pronuclear injection of a YAC containing the human PMP22
54 founder transgenic mice that was produced by pronuclear injection of an SssI-methylated construct cou
55                                              Pronuclear injection of DRAP and mutant oligonucleotides
56                                              Pronuclear injection of the 170,000-bp viral genome indu
57 rthermore, transgenic mice were generated by pronuclear injection of the modified BAC, and germline t
58 ing the entire coding region of Prss37, used pronuclear injection to create transgenic mice expressin
59 duce homologous recombinant mice directly by pronuclear injection, but the frequency of HDR was low.
60  stem (ES) cell-based targeted insertion, or pronuclear injection, of the knockdown expression casset
61 ased approach to produce transgenic mice via pronuclear injection, whereby an intact single-copy tran
62 gmyc and introduced into the mouse zygote by pronuclear injection.
63 nto the mouse as transgenes via conventional pronuclear injection.
64 e not generated after two separate rounds of pronuclear injections.
65 Conversely, androgenotes display cortex-only pronuclear interactions mimicking ICSI.
66 n zygotes that cluster their nucleoli at the pronuclear interface are thought to be more likely to de
67 ternal chromosomes eventually cluster at the pronuclear interface, in direct proximity to each other,
68 as probes to study the formation of the male pronuclear lamina in Drosophila melanogaster.
69 ologically differentiated out of a series of pronuclear masses.
70  Drosophila egg's competence to support male pronuclear maturation is acquired during activation.
71 and H3-K27 and these signals increase during pronuclear maturation.
72 cell stage arrest before entry into mitosis: pronuclear meeting occurred normally, but nuclear envelo
73                The second phase begins after pronuclear meeting, when the sperm asters begin to invad
74 y (FIB-SEM) to study the architecture of the pronuclear membranes at nanometer-scale resolution.
75 astral microtubule pulling forces to trigger pronuclear membranes scission.
76 , the parental genomes are separated by four pronuclear membranes.
77                     The transposase-enhanced pronuclear microinjection (PNI) technique described here
78 enic mouse production because of the ease of pronuclear microinjection and its overall fecundity.
79                  For Cre lines generated via pronuclear microinjection of a Cre transgene construct,
80                                              Pronuclear microinjection of ZFNs, shown by our data to
81 and reliable transgene expression to classic pronuclear microinjection or somatic cell nuclear transf
82 ransgenic L1 mouse lines are constructed via pronuclear microinjection, a procedure that typically re
83 d reliable transgene expression with classic pronuclear microinjection, and it offers comparable effi
84 d reliable transgene expression with classic pronuclear microinjection, and it offers comparable effi
85 n compares very favourably with conventional pronuclear microinjection, and report an improvement in
86 (TetOp)(7)-FasL responder mice, generated by pronuclear microinjection, were bred with Clara cell sec
87 uires fewer than half the animals needed for pronuclear microinjection.
88 lpha A-crystallin promoter were generated by pronuclear microinjection.
89 development of strategies aimed at bypassing pronuclear microinjection.
90            Transgenic mice were generated by pronuclear microinjection.
91 Cas9-mediated editing at the Avpr1a gene via pronuclear microinjections in Syrian hamsters (Mesocrice
92 of the microfilament-rich cell cortex during pronuclear migration and again during cytokinesis.
93                                              Pronuclear migration and formation of the first mitotic
94 cate that sperm asters are not essential for pronuclear migration but are required for rapid female p
95                                              Pronuclear migration does not occur in activated eggs la
96           Meiotic spindles are disorganized, pronuclear migration fails, and the mitotic apparatus fo
97 t before karyogamy in eukaryotes is known as pronuclear migration or as nuclear congression in Saccha
98                                              Pronuclear migration produces global cytoplasmic flows t
99                         Our simulations show pronuclear migration, and moreover, a robust pronuclear
100                                Specifically, pronuclear migration, centrosome separation, and centros
101  exhibit defects in chromosome condensation, pronuclear migration, kinetochore assembly, and spindle
102 ditis elegans to identify genes required for pronuclear migration, one of the first events in embryog
103 ature show defects in centrosome separation, pronuclear migration, rotation of the centrosome/nucleus
104 ltiple nuclei are well understood, including pronuclear migration, the Drosophila syncytial blastoder
105 clear envelope (NE) constituent required for pronuclear migration.
106  pronucleus-centrosome attachment and female pronuclear migration.
107  Arp2/3 mutants correlates with reduced male pronuclear migration.
108 e male pronucleus and the centrosomes during pronuclear migration.
109 key consequences of HIs for the mechanics of pronuclear migration.
110  metaphase II (MII) arrest and progressed to pronuclear, MIII, and anaphase/telophase III stages.
111  of cytoplasmically driven microtubule-based pronuclear motion in the single-celled Caenorhabditis el
112  migration but are required for rapid female pronuclear movement and premitotic positioning of the pr
113 tic spindle imaging suggests para-tangential pronuclear orientation, which initiates random spindle a
114 ygotes progress into interphase and commence pronuclear (PN) formation.
115 ication is the male pronucleus (e.g., DNA or pronuclear proteins) or some extranuclear factor from th
116                                         Male pronuclear remodeling in the injected oocytes was requir
117 f possible functions of the KLP3A protein in pronuclear specification and migration.
118 ost Setd1b(Gdf9) cKO zygotes remained in the pronuclear stage and displayed polyspermy in the perivit
119  embryo is likely determined as early as the pronuclear stage and may be predicted by a 12-gene trans
120 ere microinjected into genetically identical pronuclear stage embryos.
121 owing fertilization and progression from the pronuclear stage into mitosis.
122 erleukin-2 receptor subunit gamma (IL2RG) in pronuclear stage marmoset embryos.
123 ossums at the Tyr locus by microinjection of pronuclear stage zygotes using CRISPR/Cas9 genome editin
124 monkey oocytes, significantly upregulated in pronuclear stage zygotes, diminished in early cleaving e
125                                       At the pronuclear stage, activated Src-family PTKs became conce
126 ior to fertilization and are arrested at the pronuclear stage.
127 ply after fertilization, as the cytoplasm of pronuclear-stage zygotes is reportedly inactive.
128               Here, we show that in advanced pronuclear-stage zygotes the paternal pronucleus contain
129 c mtDNA variants have commenced or completed pronuclear transfer (and thus receipt of a mitochondrial
130                                     Maternal pronuclear transfer and CTCF mRNA microinjection experim
131              An integrated program involving pronuclear transfer and PGT was effective in reducing th
132                              We believe that pronuclear transfer between zygotes, as well as the rece
133                    Mitochondrial donation by pronuclear transfer involves transplantation of nuclear
134                                        Thus, pronuclear transfer offers affected women the potential
135 tracytoplasmic sperm injection procedure for pronuclear transfer or for PGT, respectively.
136 en generations by maternal spindle transfer, pronuclear transfer or polar body transfer: all involve
137 ood of the 8 infants whose mothers underwent pronuclear transfer ranged from undetectable to 16%.
138                                              Pronuclear transfer resulted in 8 live births and 1 ongo
139 We found that mitochondrial donation through pronuclear transfer was compatible with human embryo via
140        We offered mitochondrial donation (by pronuclear transfer) or preimplantation genetic testing
141 sfer, including spindle-chromosome transfer, pronuclear transfer, and first and second polar body tra
142 mtDNA) or elevated heteroplasmy were offered pronuclear transfer.
143 e we report the first preclinical studies on pronuclear transplantation (PNT).
144  Couples with high SPTRX3 produced fewer two-pronuclear zygotes and had a reduced pregnancy rate (19.

 
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