ライフサイエンスコーパス: protoplast

1 vitro (in nuclear extracts) and in vivo (in protoplasts).

2 the inner membrane that surrounds the spore protoplast.

3 in both N. benthamiana and maize (Zea mays) protoplasts.

4 for the accumulation of full-length PEMV in protoplasts.

5 , insertions and deletions in rice and wheat protoplasts.

6 d with individual amiRNA candidates in plant protoplasts.

7 neration of plants from Arabidopsis thaliana protoplasts.

8 pulus trichocarpa stem differentiating xylem protoplasts.

9 ivate hydrolytic enzyme promoters in soybean protoplasts.

10 fluorescence complementation in Arabidopsis protoplasts.

11 GFP transport to the plastids of Arabidopsis protoplasts.

12 across the plasma membrane of root epidermal protoplasts.

13 rs to the tonoplast in Arabidopsis mesophyll protoplasts.

14 phisticated thylakoid architecture in intact protoplasts.

15 by the CryR1 cis-element in Arabidopsis leaf protoplasts.

16 tid envelope after expression in Arabidopsis protoplasts.

17 nidase, repressed its translation in tobacco protoplasts.

18 regulator exclusively in the nucleus of rice protoplasts.

19 XXT1, XXT2, and XXT5 proteins in Arabidopsis protoplasts.

20 observed its nuclear localization in barley protoplasts.

21 e plasma membrane of tobacco Bright Yellow-2 protoplasts.

22 human cells and ADH1 in Arabidopsis thaliana protoplasts.

23 imilar to those produced in p1-5b-inoculated protoplasts.

24 support viral genome replication in tobacco protoplasts.

25 UTAMATE RECEPTOR-LIKE (GLR) channels in root protoplasts.

26 Arabidopsis (Arabidopsis thaliana) mesophyll protoplasts.

27 a gusA gene into tobacco (Nicotiana tabacum) protoplasts.

28 was shown to be a target of bZIP60 in maize protoplasts.

29 tion of a PHO1 5'UTR-luciferase construct in protoplasts.

30 of RNAi knockout genes would be feasible in protoplasts.

31 lso be utilized to examine gene functions in protoplasts.

32 a and H4b region can mediate satC fitness in protoplasts.

33 fect of the rice PHO1 uORF on translation in protoplasts.

34 Arabidopsis (Arabidopsis thaliana) mesophyll protoplasts.

35 iently transformed maize Black Mexican Sweet protoplasts.

36 protein-protein interactions in Arabidopsis protoplasts.

37 taxin of plant) 51 and SYP61, in Arabidopsis protoplasts.

38 ssypium hirsutum (cotton) leaves and arugula protoplasts.

39 transient expression analysis in Arabidopsis protoplasts.

40 K MPK4 in vitro, and activates it in vivo in protoplasts.

41 t yeast strains or electroporated into plant protoplasts.

42 n transient expression assays in Arabidopsis protoplasts.

43 says and functional studies in nontransgenic protoplasts.

44 toplasts than in pRTL2-GFP:TGBp2-transfected protoplasts.

45 ent protein (GFP) chimera in vivo in tobacco protoplasts.

46 d in isolated Arabidopsis thaliana mesophyll protoplasts.

47 x with RAV1 both in yeast and in Arabidopsis protoplasts.

48 by ZmHSFA2 and inhibited by ZmHSBP2 in maize protoplasts.

49 concentrations that were non-toxic to poplar protoplasts.

50 dependent off-target effects of CBEs in rice protoplasts.

51 ach for the tracking of rare RNAs within the protoplasts.

52 c acid probe sequences can be uptaken by the protoplasts.

53 es in response to ABA in B. napus guard cell protoplasts.

54 the SARD1 promoter in yeast and Arabidopsis protoplasts.

55 aling by transient expression in Arabidopsis protoplasts.

56 nt system was adapted to function in tobacco protoplasts.

57 Arabidopsis (Arabidopsis thaliana) mesophyll protoplasts.

58 electron microscopy, and cell separation by protoplasting.

59 At pH 5.7, the majority of auxin influx into protoplasts - 75% - was mediated by the influx carrier A

60 In contrast, in mature epidermal protoplasts a plasma membrane hyperpolarization-activate

61 on mammalian, whole plant cells, plant leaf protoplast and fungal cell cultures and observed using c

62 Protoplast and oocyte swelling assays showed that PIP2;5

63 Protoplast and test-tube reconstitution assays were used

64 athematical model, we characterized PULSE in protoplasts and achieved high induction rates, and we co

65 smotic water permeability (Pf) of guard cell protoplasts and an accumulation of reactive oxygen speci

66 raffinose biosynthesis in transformed maize protoplasts and Arabidopsis plants.

67 microscopy in Arabidopsis thaliana mesophyll protoplasts and bimolecular fluorescence complementation

68 ic plant parts using transient expression in protoplasts and dual luciferase outputs.

69 Arsenic efflux was measured from protoplasts and from intact plants, and arsenic levels w

70 nd EVR at the plasma membrane of Arabidopsis protoplasts and hypothesize that CST negatively regulate

71 a TF gene affecting wood formation, in these protoplasts and identified differentially expressed gene

72 ment inhibits TBSV RNA accumulation in plant protoplasts and in Nicotiana benthamiana leaves.

73 ed virus replication, respectively, in maize protoplasts and in plants.

74 inimal promoter in tobacco, corn and soybean protoplasts and in transgenic Arabidopsis and tobacco pl

75 e Ethylene receptor 1 (ETR1) gene in tobacco protoplasts and obtained edited regenerates.

76 Arabidopsis (Arabidopsis thaliana) protoplasts and plants were transiently and stably trans

77 protein localized to the plasma membrane in protoplasts and plasmolysis experiments, suggesting that

78 Splicing analyses of constructs in protoplasts and RNA from overexpression lines used high-

79 fully transactivates the VPE gene in soybean protoplasts and that this transactivation was associated

80 can be achieved in ~60 s, with isolation of protoplasts and their subsequent transfection taking ~50

81 AID) systems to mutagenize Nicotiana tabacum protoplasts and to regenerate plants harboring the resul

82 na benthamiana leaves, Arabidopsis mesophyll protoplasts and tobacco BY-2 protoplasts, regardless of

83 ctivates DREB2A expression in mesophyll cell protoplasts and transgenic plants and binds directly to

84 ng three different clusters of genes in rice protoplasts and verification of deletions of two cluster

85 YB305 fusion localized to nucleus of tobacco protoplasts and yeast one-hybrid assays demonstrated tha

86 h it is not irreversibly associated with the protoplast, and presumably resides outside the cell memb

87 onto polysomes and stimulates translation in protoplasts, and both processes are sensitive to TOR inh

88 rants, as well as regenerants from wild-type protoplasts, and confirmed that their genotypes were inh

89 tribution, measurement of auxin transport in protoplasts, and direct quantification of free auxin lev

90 vated the promoter of AAO3 in mesophyll cell protoplasts, and electrophoretic mobility shift assays s

91 uses cGMP-dependent net water uptake into WT protoplasts, and hence volume increases, whereas respons

92 AvrPtoB(1-307) was phosphorylated in tomato protoplasts, and mass spectrometry identified serine 258

93 synthetic parts in sorghum (Sorghum bicolor) protoplasts, and the results showed that our method work

94 ucleus in Arabidopsis (Arabidopsis thaliana) protoplasts, and VEN4 homologs are present in a wide ran

95 encoding AL1 were cotransfected into tobacco protoplasts, and viral DNA replication was monitored by

96 pts are overexpressed in cls leaves, and cls protoplasts are more sensitive to programmed cell death

97 Because protoplasts are nongrowing cells, effective RNAi-trigger

98 Wheat root protoplasts are patch clamped in the whole-cell configur

99 the preparation of bioactive COS and fungal protoplasts, as biocontrol agent against pathogenic fung

100 Both are expressed strongly in guard cell protoplasts, as determined by reverse transcription-poly

101 Here, we developed a protoplast assay to rapidly optimize the multiplexed clu

102 We use membrane photobleaching and protoplast assays to demonstrate that SpoIIIE is require

103 ility shift, yeast one-hybrid, and C. roseus protoplast assays.

104 ions across the plasma membrane of a single protoplast at multiple voltages and in complex physiolog

105 In mesophyll protoplasts, AtECA3-green fluorescent protein associated

106 Yeast- or protoplast-based two-hybrid and bimolecular fluorescent

107 ng context of cultured cell filaments and in protoplasts before and during regeneration.

108 In an Arabidopsis-protoplast beta-glucuronidase reporter gene assay, as we

109 imes less efficiently than wild-type TGMV in protoplasts but produced severe symptoms that were delay

110 t can accumulate to wild-type (wt) levels in protoplasts but remain less fit in planta when competed

111 a methodology for the isolation of idioblast protoplasts by fluorescence-activated cell sorting is es

112 rod-shaped cells are converted into spheres (protoplasts) by treatment with lysozyme, DivIVA adopts a

113 Protoplasts can be used for genome editing using several

114 e were transfected into three distinct plant protoplast cell systems.

115 conducted genome modification of embryogenic protoplast cells and epicotyl tissues.

116 Rice protoplast cells transformed with Cas9/sgRNA constructs

117 Therefore, RNAi in protoplasts complements existing genetic tools, as it al

118 Experiments with protoplasts confirmed that the coexpression of MYB77 and

119 Furthermore, a protoplast cotransfection assay showed that BBX24 and BB

120 C1 and LEC2 proteins produced in Arabidopsis protoplasts could form a ternary complex with NF-YC2 in

121 eins, when expressed in qed1 or rare1 mutant protoplasts, could not complement the editing defect.

122 In plant protoplasts, cryptochrome activation results in rapid in

123 histone variant family, were observed during protoplast culture.

124 PEG-calcium transfection of plasmid DNA and protoplast culture.

125 To investigate this, leaf protoplast cultures were cofed with 13C6-labeled conifer

126 e-cell patch-clamp measurements on root cell protoplasts demonstrated that Ag NPs slightly inhibited

127 )P level due to chemical inhibition in plant protoplasts; depletion of two PI(4)P kinases, Stt4p and

128 quired for MS channel activities detected in protoplasts derived from root cells.

129 me-wide transcript profiles of plantlets and protoplast-derived cells (PdCs) during the first week of

130 ORMATION4, is required for the initiation of protoplast division.

131 Furthermore, PSKR1-deficient protoplasts do not expand in response to PSK but are sti

132 momycin binding to intact sucrose-stabilized protoplasts, even though the drug bound normally to the

133 ting to isolate responses in the transformed protoplasts exclusively.

134 e unresponsive to PSK, and bak1-3 and bak1-4 protoplasts expanded less in response to PSK but were fu

135 PSK promotes protoplast expansion in the wild type but not in cngc17.

136 Protoplast expansion is likewise promoted by cGMP in a C

137 Protoplasting experiments indicated that both GGT1 and G

138 aize Ubiquitin promoter for use in transient protoplast expression assays and particle bombardment tr

139 responsive genes in a Daucus carota (carrot) protoplast expression system.

140 Expression of the splicing reporter in protoplasts faithfully produced all splice variants from

141 isolated epidermal peels and enlargement of protoplasts, firmly establishing a direct guard cell res

142 l disruption of mesophyl-derived Arabidopsis protoplasts, followed by a density gradient fractionatio

143 Protoplast formation and transformation takes less than

144 We isolated a total of 3 x 10(8) guard cell protoplasts from 22,000 Arabidopsis thaliana plants and

145 tilized approximately 350 million guard cell protoplasts from approximately 30,000 plants of the Arab

146 Protoplasts from BGL-1 lines divide and form calli witho

147 y in insect cell cultures and in Arabidopsis protoplasts from cryptochrome mutant seedlings.

148 me digestion of tiny leaf strips to separate protoplasts from leaf tissue and protects chloroplasts f

149 s, either by overexpression or by the use of protoplasts from the corresponding mutants.

150 e volume increases, whereas responses of the protoplasts from the receptor mutant are impaired.

151 n Kros and the osmotic water permeability of protoplasts from the veins but not from the mesophyll.

152 Finally, because we isolated protoplasts from tissues of 14-d-old seedlings instead o

153 late 50% less [(3)H]MTX than the vacuoles of protoplasts from wild-type plants when incubated in medi

154 otocol for the isolation and transfection of protoplasts from wood-forming tissue, the stem-different

155 Here we developed a protoplast fusion-based protocol for horizontal transfer

156 Here we developed a protoplast fusion-based protocol for horizontal transmis

157 Cultured guard cell protoplasts (GCP) of tree tobacco (Nicotiana glauca) com

158 Cultured tree tobacco guard cell protoplasts (GCPs) are useful for studying the effects o

159 ll surface area to volume ratio in wild-type protoplasts generates similar shape changes and cell div

160 h relies on a tight coordination between the protoplast growth and the synthesis/remodeling of the ex

161 xpression system using Arabidopsis mesophyll protoplasts has proven an important and versatile tool f

162 etion of a targeted polypeptide and, because protoplasts have a limited life span, if functional assa

163 In transiently transfected Arabidopsis protoplasts, HYL1-mCherry and YFP-DCL1 fusion proteins c

164 that entailed fusion of recipient and donor protoplasts, hyphal anastomosis, and single-conidium iso

165 tobacco (Nicotiana tabacum) Bright Yellow-2 protoplasts identified single cDNA clones encoding prote

166 ed SIZ1-mediated sumoylation in vitro and in protoplasts identifying the K393 residue as the principa

167 A did not accumulate to detectable levels in protoplasts in the absence of the kl-TSS.

168 lso exhibits polar localization in spherical protoplasts, in contrast to other polarity proteins so f

169 the cellular level using vitality stains in protoplasts, in intact seedlings grown on agar plates an

170 te ring mechanisms, we studied fission yeast protoplasts, in which constriction occurs without the ce

171 Overexpression of ZmDREB2A in maize protoplasts increased the expression of ZmGH3.2, ZmRAFS

172 ar fractionation of pea (Pisum sativum) leaf protoplasts indicated that 30% of lysophosphatidylcholin

173 Arabidopsis (Arabidopsis thaliana) mesophyll protoplasts indicated that a combination of the yeast Ga

174 XXT2YFP and XXT5HA proteins from Arabidopsis protoplasts indicated that while the formation of the XX

175 scription factor in vitro and in Arabidopsis protoplasts, indicating that ABI5 is sumoylated through

176 After cleavage of the protoplast into aplanospores, a vacuole and lipids remai

177 to the host cells, the parasite injected its protoplast into the host between the host cell wall and

178 Transient genetic modification of plant protoplasts is a straightforward and rapid technique for

179 BASL polarity within protoplasts is dynamic and resistant to auxin transport

180 Cell division in protoplasts is enhanced 7-fold in the presence of exogen

181 Radiolabeled auxin uptake was measured in protoplasts isolated from roots of Arabidopsis thaliana.

182 The vacuoles of protoplasts isolated from the leaves of Wassilewskia atm

183 es of control experiments, establishing that protoplast isolation and cell sorting procedures did not

184 The protoplast isolation and DNA transfection procedures tak

185 ue preparation, digestion of SDX cell walls, protoplast isolation and DNA transfection.

186 The core procedure, from protoplast isolation to identification of optimal amiRNA

187 The method includes protoplast isolation, PEG-calcium transfection of plasmi

188 The whole procedure, including protoplast isolation, takes approximately 6 h.

189 behavior when expressed in tobacco mesophyll protoplasts: KAT2 forms homotetrameric channels active a

190 In addition to protoplasts, leaf epidermal cells transiently transforme

191 morphology of avenaciolide-treated cells was protoplast-like, which indicated that cell wall biosynth

192 r the pectin activation of numerous genes in protoplasts, many of which are involved in cell wall bio

193 rinated analogue of oritavancin, to isolated protoplast membranes and whole-cell sucrose-stabilized p

194 In isolated protoplast membranes, both with and without 1 M sucrose

195 show that overexpressed Ggamma1 localizes to protoplast membranes, but Gbeta exhibits membrane locali

196 ssociated vacuoles are detected in mesophyll protoplasts of des1 mutants.

197 of preassembled Cas9 ribonucleoproteins into protoplasts of F. proliferatum.

198 eq to obtain single-cell transcriptomes from protoplasts of more than 10,000 Arabidopsis (Arabidopsis

199 membranes and whole-cell sucrose-stabilized protoplasts of Staphylococcus aureus, grown in media con

200 ry) was aberrant in roots and root epidermal protoplasts of the Atann1 knockout mutant.

201 IA PLA(2) activity toward cell wall-depleted protoplasts of the wt and tagO strains of S. aureus or i

202 GFP-intron-GFP reporter was investigated in protoplasts of three single and three double mutants of

203 Our functional results in Zea mays mesophyll protoplasts on ABA-inducible expression effects on the L

204 ative SYP121-Sp2 fragment in maize mesophyll protoplasts or epidermal cells leads to a decrease in th

205 ed either volume changes of single hypocotyl protoplasts or hypocotyl growth, both at high temporal r

206 ed TBSV replication in Nicotiana benthamiana protoplasts or in whole plants.

207 Transient gene expression, in plant protoplasts or specific plant tissues, is a key techniqu

208 (Solanum tuberosum) regenerated from either protoplasts or stem explants for copy number changes by

209 tabolites in Arabidopsis thaliana guard cell protoplasts over a time course of ABA treatment.

210 Protoplasts overexpressing both AtHD2B and RPS6 exhibite

211 In protoplasts pectins activate, in a WAK2-dependent fashio

212 ur protocol gives a high yield (~2.5 x 10(7) protoplasts per g of SDX) of protoplasts sharing 96% tra

213 Nontransgenic genome editing in regenerable protoplasts, plant cells free of their cell wall, could

214 enhance virus accumulation in the inoculated protoplasts, promote cell-to-cell virus movement in the

215 Metabolite profiling of the cofed protoplasts provided strong support for the occurrence o

216 occurred in more than 10% of the transformed protoplasts regardless of the reporter's chromosomal pos

217 opsis mesophyll protoplasts and tobacco BY-2 protoplasts, regardless of whether VirE2 was co-expresse

218 ted by cuttings displayed no changes, all 15 protoplast regenerants tested were affected by aneuploid

219 As protoplasts regenerate, polarity remains dynamic in isot

220 c mutation did not alter viral DNA levels in protoplast replication assays.

221 a membrane face of elongation zone epidermal protoplasts resulted in the appearance of a hyperpolariz

222 oduction of these four components into plant protoplasts results in ABA-responsive gene expression.

223 A transcriptional activation assay of protoplasts revealed that ABA treatment and coexpression

224 Reporter-fusion analyses in protoplasts revealed that, with a free N terminus, PGD1

225 -cell configuration analysis of pollen grain protoplasts revealed three subpopulations of anionic cur

226 a indicate that APY expression in guard cell protoplasts rises quickly when these cells are moved fro

227 culum (ER) in stably transformed Arabidopsis protoplasts, roots and root hairs.

228 Isolated protoplasts serve as a transient expression system that

229 d (~2.5 x 10(7) protoplasts per g of SDX) of protoplasts sharing 96% transcriptome identity with inta

230 FP-OsPIP1;3 alone in Xenopus oocytes or rice protoplasts showed OsPIP1;3 mislocalization in the endop

231 Transient co-transfections in Arabidopsis protoplasts showed that A-ZIP53 inhibited three bZIPs an

232 ity assays performed in Arabidopsis and rice protoplasts showed that OsPCF2 and OsNIN-like4 are activ

233 Patch clamp recordings on moss protoplasts showed the presence of three distinct thermo

234 by spongy mesophyll anatomy, decreasing with protoplast size and increasing with airspace fraction an

235 ast two-hybrid analysis and Arabidopsis leaf protoplast split luciferase assay, to demonstrate that m

236 Furthermore, expression of FLDK3R in fld protoplasts strongly reduced FLC transcription compared

237 previous results carried out in transfected protoplasts suggest that the hormone auxin can be bypass

238 in the K+ flux-to-current ratio among single protoplasts suggests a heterogeneous distribution of KOR

239 ther cell engulfs the forespore to produce a protoplast, surrounded by two bilayer membranes, which s

240 stem overcomes the drawback that transfected protoplast suspensions are often a heterogeneous mix of

241 elated mutants and Coimbra, no auxin-induced protoplast swelling occurred.

242 be involved in mediating rapid auxin-induced protoplast swelling, but it is not involved in the contr

243 ated the TIR1/AFB pathway but did not induce protoplast swelling; instead, it showed auxin activity i

244 Using a parsley (Petroselinum crispum) protoplast system and a modified reporter gene vector wi

245 Furthermore, we developed a cotton protoplast system for transient gene expression to study

246 itor-responsive gene expression in a parsley protoplast system.

247 s investigated using an Arabidopsis thaliana protoplast system.

248 P fluorescence was greater in virus-infected protoplasts than in pRTL2-GFP:TGBp2-transfected protopla

249 teraction by luminescence within Arabidopsis protoplasts that express recombinant proteins at physiol

250 t expression system in Arabidopsis mesophyll protoplasts that is highly amenable for the dissection o

251 We find that ACD2 shields root protoplasts that lack chlorophyll from light- and PPIX-i

252 enome edits when ssODNs were introduced into protoplasts that were pretreated with the glycopeptide a

253 ransgenic plants and transient expression in protoplasts, the impact of cis-NAT expression on mRNA tr

254 planta as in the moss Physcomitrella patens protoplasts, the presence of RY-like (RYL) elements is n

255 sider the case for local equilibrium between protoplasts, their cell walls, and adjacent air spaces d

256 In assays with transfected protoplasts, this repression was previously shown to occ

257 anslation in both wheat germ extract and oat protoplasts through a novel, noncanonical translation me

258 ethod for the transformation of N. uniformis protoplasts to inactivate both nocK and nocL was develop

259 A protocol was developed with isolated protoplasts to obtain peripheral chloroplasts (P-CP), a

260 tin immunoprecipitation-quantitative PCR and protoplast trans-activation assays were used to show tha

261 cane internode nuclear protein extracts, and protoplast transactivation assays demonstrated that ShMY

262 rmine binding sites in target promoters, and protoplast transactivation assays to demonstrate domains

263 Protoplasts transfected with PVX-GFP:TGBp2 or pRTL2-GFP:

264 Protoplast transfection and regeneration systems are use

265 Arabidopsis protoplast transfection assays suggested that PtrMYB152

266 We showed that protoplasts transfection with an in vitro-synthesized ds

267 g toolkits for citrus including PEG-mediated protoplast transformation, a GFP reporter system that al

268 Confocal microscopy of protoplasts transformed with enhanced green fluorescence

269 by quantitative real-time PCR (qRT-PCR) and protoplast transient assay.

270 Using a plant protoplast transient expression analysis we have further

271 ctionally important because Glc signaling in protoplast transient expression assays is compromised by

272 s of mRNA levels, promoter-GUS activity, and protoplast transient expression showed that the expressi

273 AtPTB1 and AtPTB2 was analysed in an in vivo protoplast transient expression system with a novel mini

274 oplasts, but also localized to nuclei during protoplast transient expression.

275 Protoplasts transiently cotransfected with promoter-luci

276 using fluorescence microscopy of A. thaliana protoplasts transiently expressing the N-terminal fusion

277 ession assays in tobacco (Nicotiana tabacum) protoplasts, TSAR1 and TSAR2 exhibit different patterns

278 expression assays in Arabidopsis and lettuce protoplasts using a flagellin-based peptide.

279 ing assays in Arabidopsis thaliana mesophyll protoplasts using green fluorescent protein fusions, and

280 Sucrose solution visibly preserved the protoplast viability and slightly influenced the water d

281 he ABA activation of PLDalpha1 in leaves and protoplasts was attenuated in the SPHK mutants, and the

282 1)P, the nascent peptidoglycan of the intact protoplasts was confined to the membrane surface.

283 protein-tagged CHX20 expressed in mesophyll protoplasts was localized mainly to membranes of the end

284 SIZ1-dependent sumoylation of ICE1 in protoplasts was moderately induced by cold.

285 , translation in tobacco (Nicotiana tabacum) protoplasts was repressed by those constructs containing

286 VAMP722 was significantly increased when the protoplasts were incubated in the light.

287 The viable protoplasts were isolated from green cotyledons, etiolat

288 s restored in an auxin-dependent manner when protoplasts were transfected with a 35S:ARF7 effector ge

289 Protoplasts were transformed with BA-mgfp5-ER, in which

290 Root cell protoplasts were used to demonstrate that signalling to

291 to the cytoplasm of tobacco and Arabidopsis protoplasts, whether in the absence or presence of VIP1

292 tors that make precise genomic edits in rice protoplasts while minimizing untargeted mutagenesis.

293 constitutively enhanced the Pf of guard cell protoplasts while suppressing its ABA-dependent activati

294 activity was measured by first transforming protoplasts with a DNA vector in a 96-well plate.

295 e constitutively or inducibly coexpressed in protoplasts with amiRNA candidates targeting single or m

296 Transfection of Arabidopsis orrm1 mutant protoplasts with constructs encoding a region encompassi

297 we could regenerate whole plants from edited protoplasts without employing selection.

298 e extracellular domain and in experiments in protoplasts without primary cell walls.

299 Herein, we tested if transient RNAi in protoplasts would result in the depletion of a targeted

300 When expressed alone in mesophyll protoplasts, ZmPIP2s are efficiently targeted to the pla