ライフサイエンスコーパス: psoralen

1 ific photoadduct formation by the conjugated psoralen.

2 on, we tested dimeric bis-PNAs conjugated to psoralen.

3 henylphosphine oxide-1, or 5-(4-phenylbutoxy)psoralen.

4 ICLs) induced by drugs such as cisplatin and psoralen.

5 t to angelicin but eliminated recruitment to psoralen.

6 account for some of the mutations induced by psoralens.

7 Psoralen 4 (Pso4) is an evolutionarily conserved protein

8 immunosuppressant PAP-1 (5-(4-phenoxybutoxy)psoralen), a high-affinity blocker of Kv1.3 channels in

9 The assay is based on mutagenesis by psoralen, a photoactive DNA cross-linker.

10 t, largely accounting for the differences in psoralen accessibility between active and inactive genes

11 l are transcribed and maintained in an open, psoralen-accessible conformation.

12 enhanced effectiveness of the clamp ODNs to psoralen activation.

13 inked to psoralen, have been shown to direct psoralen adduct formation in cells, leading to mutagenes

14 , neither XPA nor XPC is cross-linked to the psoralen adduct.

15 nd are required for XPD cross-linking to the psoralen-adducted base, neither XPA nor XPC is cross-lin

16 excision nuclease assembled at the site of a psoralen-adducted thymine.

17 We previously demonstrated that psoralen adducts targeted by triple-helix-forming oligon

18 Removal of psoralen adducts was blocked in XPC-deficient cells but

19 , a more rapid removal of digoxigenin-tagged psoralen adducts, and decreased cellular sensitivity to

20 e helices, even in the absence of associated psoralen adducts, are able to provoke DNA repair and cau

21 t mutations induced by third-strand-directed psoralen adducts.

22 XPC protein was rapidly recruited to psoralen adducts.

23 In addition to the ICL formation, psoralens also readily form various monoadducts (MAs) wi

24 or pathogen inactivation using the synthetic psoralen amotosalen HCl.

25 re densely biotinylated using a biotinylated psoralen analog plus UVA light and hybridized on microar

26 NEIL3 is the primary unhooking mechanism for psoralen and abasic site ICLs.

27 further characterized the inhibition of four psoralen and coumarin derivatives toward ALDH2 and compa

28 The TFOs were linked to psoralen and designed to target and mutagenize a site in

29 ast cancer followed by Bergapten, Angelicin, Psoralen and Isoimperatorin.

30 Psoralen and its derivatives are widely applied for the

31 sensitive to photochemical inactivation with psoralen and long-wavelength ultraviolet light.

32 he collagenase gene upon treatment with free psoralen and subsequent photoactivation.

33 In this study, APS165 was modified with psoralen and the extent of triplex formation with alpha1

34 tation equilibrium of 3'-linked intercalated psoralen and to develop conditions that lead to preferen

35 Sunlight and psoralen and ultraviolet A (PUVA) are risk factors for t

36 Psoralen and ultraviolet A light (PUVA) are used to kill

37 es that themselves had never been exposed to psoralen and ultraviolet A radiation.

38 king the ciclosporin who had been exposed to psoralen and ultraviolet-A light (PUVA) and other treatm

39 elix-forming oligonucleotides were linked to psoralen and used to form triplexes on the plasmids.

40 tive to photochemical inactivation with S-59 psoralen and UV light.

41 om 30 minute reactions were crosslinked with psoralen and UV, linearized with restriction enzymes and

42 with photochemical therapy (PCT) using S-59 psoralen and UVA light to prevent proliferation.

43 s are widely consumed foods that are rich in psoralens and furocoumarins, a group of naturally occurr

44 A combination of psoralens and ultraviolet A radiation is widely used to

45 patients with severe psoriasis treated with psoralens and ultraviolet-A therapy (PUVA) who enrolled

46 from Xenopus laevis has been shown to cleave psoralen- and abasic site-induced ICLs in Xenopus egg ex

47 ary using this system identified the angular psoralen angelicin and the insect growth regulator fenox

48 Psoralens are natural compounds that serve in the light

49 DNA damage, thus ICL-inducing agents such as psoralen, are clinically useful chemotherapeutics.

50 Upon near-ultraviolet irradiation (360 nm), psoralen-Asp41-Tat(37-72) cross-linked to a single site

51 The psoralen-bis-PNA conjugate was found to direct photoaddu

52 ks was dependent on precise placement of the psoralen by the triple helix-forming oligonucleotide at

53 Photoreactive psoralens can form interstrand crosslinks (ICLs) in doub

54 Upon exposure to UVA light, psoralens can induce DNA interstrand cross-links (ICLs),

55 s (TFOs) linked to DNA damaging agents (e.g. psoralen) can stimulate HR, providing the potential to i

56 We show that psoralen-conjugated pcPNAs can deliver site-specific pho

57 imethylaminopropylamine, in a 10-nucleotide, psoralen-conjugated TFO confers substantial increases in

58 Using a psoralen-conjugated TFO designed to bind to a site overl

59 was induced efficiently by injection of both psoralen-conjugated TFOs (followed by long-wave UVA ligh

60 Psoralen-conjugated triplex-forming oligonucleotides (ps

61 Interactions between nuclease-resistant, 5'-psoralen-conjugated, chimeric methylphosphonate oligodeo

62 of pyrimidine deoxyribo- or 2'-O-methylribo-psoralen-conjugated, triplex-forming oligonucleotides, p

63 These results indicate that TFO-linker-psoralen conjugates allow simultaneous, efficient target

64 ree, two, or no strands covalently linked by psoralen conjugation and photoaddition.

65 A defined template DNA with a single psoralen cross-link and the SV40 origin of replication w

66 The psoralen cross-link defines the intramolecular interacti

67 The psoralen cross-link inhibited DNA replication by termina

68 ation of TBP from DNA was not prevented by a psoralen cross-link positioned immediately preceding the

69 The major pathway of psoralen cross-link repair in yeast is error-free and in

70 ontaining four adjacent cytosines, next to a psoralen cross-link site.

71 A site-specific psoralen cross-link was placed between the promoter and

72 porter gene, we showed that a single defined psoralen cross-link was removed in repair-proficient cel

73 l incisions 5' to the cross-linked base of a psoralen cross-link, generating a gap of 22 to 28 nucleo

74 We compared psoralen cross-link, psoralen monoadduct, and DSB repair

75 epair of plasmid molecules carrying a single psoralen cross-link, psoralen monoadduct, or double-stra

76 UV-induced psoralen cross-linking is used to probe the secondary st

77 Nuclease digestion and psoralen cross-linking mapped the PIC between positions

78 understand the relationship between triplex psoralen cross-linking products and the fate of RNA Pol

79 ed after UV irradiation, which initiates the psoralen cross-linking reaction.

80 These studies have low sensitivity, because psoralen cross-linking suggested few (estimated <10%) of

81 embedded sequences for triplex formation and psoralen cross-linking.

82 e characterized by elevated accessibility to psoralen cross-linking.

83 In the yeast Saccharomyces cerevisiae, psoralen cross-links are processed by nucleotide excisio

84 Our observations suggest that psoralen cross-links can be repaired by three pathways:

85 Psoralen cross-links induced both recombination and muta

86 comparison, the ability of triplex-directed psoralen cross-links to induce recombination was only pa

87 Site-specific mutagenesis by psoralen cross-links was dependent on precise placement

88 repair of laser-localized lesions formed by psoralen (cross-links/monoadducts) and angelicin (only m

89 Inhibition of transcription by psoralen-cross-linked triplexes results in both arrest a

90 x DNA containing a site-directed interstrand psoralen crosslink.

91 Chloroquine titration, psoralen crosslinking and supercoiling-sensitive reporte

92 develop PARIS, a method based on reversible psoralen crosslinking for global mapping of RNA duplexes

93 A light-induced psoralen crosslinking reaction was used to attach a puro

94 ve fraction from yeast nuclei, and selective psoralen crosslinking was used to distinguish between ac

95 ld be detected only after analysis following psoralen crosslinking.

96 demonstrate that TFOs can be used to direct psoralen crosslinks adjacent to a gene as a way of activ

97 protection assay to detect triplex-directed psoralen crosslinks in genomic DNA prepared from TFO-tra

98 NEIL1 recruitment to psoralen crosslinks was independent of the nucleotide ex

99 blocked by infrequent, randomly distributed psoralen crosslinks, but the bacterial population was ab

100 se that RPA70 makes the initial contact with psoralen-damaged DNA but that within preincision complex

101 omplex is formed, containing HMGB1, RPA, and psoralen-damaged DNA.

102 Using a psoralen delivery system mediated by a DNA third strand

103 For one psoralen derivative, 4'-aminomethyl-4,5',8-trimethylpsor

104 Some new psoralen derivatives were synthesized and evaluated as i

105 Among the psoralen derivatives, 8-methoxypsoralen (8-MOP) is conve

106 A glycosylases Nei and NEIL1 excise unhooked psoralen-derived ICLs in three-stranded DNA via hydrolys

107 lusion, the Nei-like DNA glycosylases unhook psoralen-derived ICLs in various DNA structures via a ge

108 ikingly, crosslinking of the siRNA duplex by psoralen did not completely block RNA interference, indi

109 WRN facilitates ATM activation in cells with psoralen DNA cross-link-induced collapsed replication fo

110 n control cells 6 and 16 h after exposure to psoralen DNA cross-links.

111 ay of NER is critical for the recognition of psoralen DNA ICLs in the mammalian genome.

112 PA has also been suggested to play a role in psoralen DNA interstrand cross-link (ICL) repair, but a

113 ucts low-efficiency nonmutagenic bypass of a psoralen DNA interstrand cross-link (ICL), whose structu

114 age recognition proteins to triplex-directed psoralen DNA interstrand cross-links (ICLs).

115 -forming oligonucleotides (TFOs) to direct a psoralen-DNA interstrand cross-link (ICL) to a specific

116 TPE can initiate photochemistry resulting in psoralen-DNA photoadducts, target DNA sequences were inc

117 DNA from irradiated cells treated with psoralen exhibited a 4- to 7-fold increase in tritium ac

118 tion, 8-p-anisyl-5-p-cyanophenyl-substituted psoralen exhibits aggregation-induced emission propertie

119 cs on platelet membrane extracts showed that psoralen forms adducts with unsaturated carbon bonds of

120 h purine and pyrimidine TFOs, when linked to psoralen, have been shown to direct psoralen adduct form

121 L with a greater efficiency than that of the psoralen ICL alone.

122 designed a model substrate DNA with a single psoralen ICL at a three-way junction (Y-shaped DNA), whi

123 f a DNA substrate containing a site-specific psoralen ICL can be monitored in vitro.

124 Using laser-induced psoralen ICL formation in cells, we find that FANCA inte

125 A and PCNA are involved in an early stage of psoralen ICL processing.

126 d MUS81-EME1 incises DNA at the 5' side of a psoralen ICL residing in fork structures.

127 generated a defined substrate with a single psoralen ICL that mimics a postincision structure genera

128 triplex technology to direct a site-specific psoralen ICL to a target DNA substrate to determine whet

129 the UvrABC nuclease cleaved the TFO-directed psoralen ICL with a greater efficiency than that of the

130 Mechanistically, NEIL3 is recruited to psoralen-ICL in a rapid, PARP-dependent manner.

131 epistatic with both NEIL3 and FA pathways in psoralen-ICL repair, suggesting that TRAIP may function

132 IL3 and function within the NEIL3 pathway in psoralen-ICL repair.

133 nd the FA/BRCA pathways are non-epistatic in psoralen-ICL repair.

134 NEIL3 pathway is the major pathway to repair psoralen-ICL through a unique DSB-free mechanism in huma

135 3 pathway is the major pathway for repairing psoralen-ICL, and the FA/BRCA pathway is only activated

136 However, how TFO-directed psoralen ICLs (Tdp-ICLs) are recognized and processed in

137 1 (HMGB1) protein bound to triplex-directed psoralen ICLs (TFO-ICLs) in vitro, cooperatively with NE

138 ors believed to be involved in the repair of psoralen ICLs [xeroderma pigmentosum (XP)-A, XP-C, XP-F,

139 simultaneously, demonstrating not only that psoralen ICLs are recognized by XPC-hHR23B alone, but al

140 The induction of p53 phosphorylation by psoralen ICLs did not require factors believed to be inv

141 kade of transcription and DNA replication by psoralen ICLs during S-phase elicits a strong apoptotic

142 Here, we show that processing of psoralen ICLs in mammalian cell extracts is dependent up

143 The processing of psoralen ICLs is also dependent upon the nucleotide exci

144 protein complexes were also observed to bind psoralen ICLs simultaneously, demonstrating not only tha

145 binds with high affinity and specificity to psoralen ICLs, and interacts with the essential NER prot

146 eta complex is shown to specifically bind to psoralen ICLs, and this binding is stimulated by the add

147 sults demonstrate that XPC-hHR23B recognizes psoralen ICLs, which have a structure fundamentally diff

148 air (MMR) complex MutSbeta were sensitive to psoralen ICLs.

149 clease UvrABC plays a key role in processing psoralen ICLs.

150 Importantly, the NEIL3 pathway repairs psoralen-ICLs without generating double-strand breaks (D

151 f a Holliday junction, suggesting a role for psoralen in the mechanism to initiate repair of psoralen

152 sing the docked poses of 29 (the most active psoralen in the series) as templates for alignment of th

153 nocytogenes as a model platform, recombinant psoralen-inactivated Lm DeltauvrAB vaccines induced pote

154 followed by G1 (G3/G1) VLPs, or with live or psoralen-inactivated SA11.

155 oxidative DNA damage, as well as trioxsalen (psoralen)-induced DNA interstrand crosslinks, but not to

156 Here we report that human NEIL3 cleaves psoralen-induced DNA-DNA cross-links in three-stranded a

157 rthermore, while Nei and NEIL1 also cleave a psoralen-induced four-stranded DNA substrate to generate

158 The HMT-adduct of d(CCGCTAGCGG) forms a psoralen-induced Holliday junction, showing for the firs

159 Psoralen-induced ICLs blocked transcription and replicat

160 produce monoadducts and ICLs and found that psoralen-induced ICLs induced phosphorylation of the Ser

161 CJ recruitment to laser-induced DSBs but not psoralen-induced ICLs is dependent on nuclease-active MR

162 l cycle arrest and genomic instability after psoralen-induced ICLs.

163 136R, and E181K, to oxidative DNA damage and psoralen-induced interstrand crosslinks was differential

164 p53 and apoptosis much more effectively than psoralen-induced monoadducts.

165 photoadducts directed by PNAs conjugated to psoralen-induced mutations at frequencies in the range o

166 In particular, using the N-4 linker, psoralen interaction with the residues of the non-coding

167 in production of incisions at the site of a psoralen interstrand cross-link and that in Fanconi anem

168 oduction of the 5' incision at the site of a psoralen interstrand cross-link as well as the 3' incisi

169 Here, we show that a single psoralen interstrand cross-link induces DNA synthesis in

170 ps of psoralen photoadducts: monoadducts and psoralen interstrand cross-link products.

171 ' and 3' incisions that occur at a site of a psoralen interstrand cross-link.

172 nuclei, specifically binds to DNA containing psoralen interstrand cross-links and that the FANCA, FAN

173 The repair of psoralen interstrand cross-links in the yeast Saccharomy

174 To elucidate factors affecting TFO-directed psoralen interstrand crosslink (ICL)-induced recombinati

175 vity, purified NEIL1 protein bound stably to psoralen interstrand crosslink-containing synthetic olig

176 cient in the stimulation of DNA synthesis by psoralen interstrand crosslinks.

177 Although the presence of psoralen is not required for targeting nor is it alone a

178 mage, are in close physical proximity to the psoralen lesion and thus are cross-linked to the damaged

179 XPF-ERCC1 complex makes an incision 5' to a psoralen lesion on Y-shaped DNA in a damage-dependent ma

180 the position of its subunits relative to the psoralen lesion.

181 r a 72 h period, suggesting that TFO-induced psoralen lesions are not repaired on this time scale.

182 ralen in the mechanism to initiate repair of psoralen-lesions in mammalian DNA.

183 Psoralens linked to triplex-forming oligonucleotides (ps

184 We have constructed chemically modified, psoralen-linked TFOs that mediate site-specific mutagene

185 We have described psoralen-linked TFOs with 2'-O-methyl and 2'-O-(2-aminoe

186 We have prepared psoralen-linked TFOs with varying amounts of the AE-modi

187 site was greatly improved by shortening the psoralen linker.

188 We combined in vivo psoralen-mediated RNA-RNA crosslinking and high-throughp

189 o-severe disease if they had been prescribed psoralen, methotrexate, cyclosporine, acitretin, adalimu

190 at(42-72) and Tat(37-72), and incorporated a psoralen-modified amino acid at position 41 during solid

191 Psoralen-modified TFOs and UVA irradiation were used to

192 We have used psoralen-modified triplex-forming oligonucleotides (TFOs

193 Psoralen-modified triplex-forming oligonucleotides (TFOs

194 Psoralen-modified triplex-forming oligonucleotides (TFOs

195 onucleotide third strands with a 3'-terminal psoralen moiety attached by linkers that differ in lengt

196 for G-quadruplex binders characterized by a psoralen moiety.

197 igonucleotides attached with a photoreactive psoralen molecule (psoTFO) can be used to induce site-sp

198 We compared psoralen cross-link, psoralen monoadduct, and DSB repair using plasmid substr

199 cules carrying a single psoralen cross-link, psoralen monoadduct, or double-strand break in yeast cel

200 ence, the kinetics of formation and yield of psoralen monoadducts and crosslinks with pyrimidine resi

201 Target DNA acquired strand-specific psoralen monoadducts in a light dose-dependent fashion.

202 nnel blockers Margatoxin, 5-(4-Phenoxybutoxy)psoralen, or broad-spectrum K(+) channel blocker 4-Amino

203 compound of this series, 5-(4-phenoxybutoxy)psoralen (PAP-1), blocks Kv1.3 in a use-dependent manner

204 Most of the induced psoralen-pcPNA mutations were single-base substitutions

205 Such psoralen-peptide conjugates provide a new class of probe

206 derived from photoaffinity cross-linking by psoralen, phenphi (cis-Rh(1,10-phenanthroline)(9,10-phen

207 GM847, GM847-Tert, and WI-38 VA13 ALT cells, psoralen photo-cross-linked in situ, and the telomere re

208 te the mechanism of photoadduct-formation by psoralen photo-cross-linking, triplex structures were ge

209 Gene activation was dependent on psoralen photo-crosslinking.

210 These results indicate that psoralen photo-crosslinks play a prominent role in activ

211 Psoralen photoadduct formation was shown to be essential

212 eriments that monitored the formation of the psoralen photoadducts also suggested that the length of

213 Northern blots indicated that TFO-directed psoralen photoadducts blocked progression of RNA polymer

214 ate the mechanism for creating site-specific psoralen photoadducts in a target duplex DNA.

215 he only pathway that can metabolize targeted psoralen photoadducts into recombinagenic intermediates.

216 e mechanism associated with the formation of psoralen photoadducts that are directed by psoTFO during

217 soTFOs) were designed to deliver UVA-induced psoralen photoadducts to two distinct sequences within t

218 The formation of these psoralen photoadducts was also photoreversible through e

219 The psoralen photoadducts were then analyzed after UV irradi

220 DNA duplex generated two distinct groups of psoralen photoadducts: monoadducts and psoralen interstr

221 site-specific cross-linking method based on psoralen photochemistry to determine the effect of core

222 was investigated by micrococcal nuclease and psoralen photocrosslinking.

223 d for their ability to form triplex-directed psoralen photoproducts with both the mutant T residue of

224 Psoralen photoreacts with DNA to form interstrand cross-

225 ultimately offering the potential to define psoralen plus ultraviolet A dosage regimes in terms of m

226 nificantly altered the threshold response to psoralen plus ultraviolet A erythema but not the rate of

227 etectable effect on the maximum slope of the psoralen plus ultraviolet A erythema dose-response curve

228 aviolet-A-emitting fluorescent tubes used in psoralen plus ultraviolet A therapy.

229 Although psoralen plus ultraviolet A treatment is highly effectiv

230 ed green tea extract per cm2 30 min prior to psoralen plus ultraviolet A treatment resulted in an alm

231 the standard dose used in photochemotherapy (psoralen plus ultraviolet A).

232 resulted in an almost complete abrogation of psoralen plus ultraviolet A-induced erythema.

233 sfully in combination with ultraviolet B and psoralen plus ultraviolet A.

234 Psoriatic patients undergoing psoralen plus ultraviolet radiation (PUVA) therapy are s

235 The Psoralen plus Ultraviolet-A (PUVA) cohort study has been

236 are hypersensitive to DNA damage induced by psoralen plus UVA irradiation (PUVA) or UVC radiation, s

237 Psoralen plus UVA light (PUVA) is commonly used to treat

238 argeting in the episomal shuttle vector, the psoralen-PNA-induced mutation frequency was 0.13%, 3.5-f

239 Using UV/psoralen (Ps)-inactivated virus to block viral transcrip

240 orming oligonucleotides (TFOs) that target a psoralen (pso) interstrand crosslink to a specific chrom

241 ple helix-forming oligonucleotides linked to psoralen (pso-TFO) to introduce a DNA interstrand cross-

242 helix-forming DNA oligonucleotides linked to psoralen (psoTFOs) were designed to deliver UVA-induced

243 a DNA interstrand crosslinking (ICL) agent, psoralen (pTFO-ICLs), to improve the gene targeting effi

244 and the KV1.3/1.5 blocker 5-(4-phenylbutoxy)psoralen reduced H2O2-elicited dilation to a similar ext

245 Photochemical treatment (PCT) with the psoralen S-59 and long wavelength ultraviolet light (UVA

246 tarting from a bromo-triflato-functionalized psoralen scaffold.

247 We used these psoralen-Tat conjugates to form specific complexes with

248 Psoralen-TFO conjugates formed DNA cross-links with high

249 We conclude that directing DNA damage with psoralen-TFOs is an efficient and specific means for blo

250 of cells after transfection with plasmid and psoralen-TFOs produced photoadducts inside the cells and

251 gments containing either an abasic site or a psoralen-thymine monoadduct.

252 rate the ability of bis-PNAs conjugated with psoralen to mediate site-specific gene modification, and

253 UV-A and UV-B, when used in combination with psoralens, topical corticosteroids, vitamin D analogues,

254 unctional survival assays indicated that the psoralen-TPE treatment was not toxic to cells.

255 e time of maximal erythema, the slope of the psoralen ultraviolet A dose-response curve was approxima

256 we determined that only 67% of mean maximum psoralen ultraviolet A erythemal intensity had developed

257 If assessment of psoralen ultraviolet A erythemal sensitivity had been ma

258 gs, it seems appropriate to consider whether psoralen ultraviolet A minimal phototoxic dose measureme

259 r to determine the separate contributions of psoralen + ultraviolet A and other ultraviolet exposures

260 here was some evidence that high exposure to psoralen + ultraviolet A before a first basal cell carci

261 Risk increased with high psoralen + ultraviolet A exposure prior to the first squ

262 s with low pre-first squamous cell carcinoma psoralen + ultraviolet A exposure, 95% confidence interv

263 Long-term, high-dose exposure to psoralen + ultraviolet A is associated with an increased

264 lop a squamous cell carcinoma after starting psoralen + ultraviolet A therapy should be closely monit

265 d 258 a basal cell carcinoma after beginning psoralen + ultraviolet A therapy.

266 rates of post-first squamous cell carcinoma psoralen + ultraviolet A treatment also were associated

267 Higher post-first tumor psoralen + ultraviolet A treatment rates also increased

268 This study's data suggest that psoralen + ultraviolet A-induced p53 mutations may play

269 he development of nonmelanoma skin cancer in psoralen + ultraviolet A-treated patients, but these mut

270 Psoralen + ultraviolet A-treated psoriasis patients appe

271 Psoralen + ultraviolet A-treated psoriasis patients are

272 carcinomas were studied in a cohort of 1380 psoralen + ultraviolet A-treated psoriasis patients pros

273 ultraviolet B for skin cancer development in psoralen + ultraviolet A-treated psoriasis patients.

274 let-type mutations, two (5%) tumors had only psoralen + ultraviolet A-type mutations, and 18 (49%) tu

275 Interestingly, psoralen + ultraviolet A-type p53 mutations were more fr

276 ising in patients with high-dose exposure to psoralen + ultraviolet A.

277 er risk among patients who have discontinued psoralen+ultraviolet A and the risk of a first tumor wit

278 In conclusion, substantial exposure to psoralen+ultraviolet A dramatically increases the risk o

279 ents because the cancer risk associated with psoralen+ultraviolet A is persistent.

280 onmelanoma skin cancer and prior exposure to psoralen+ultraviolet A remains an important issue in the

281 5 years, about 7% of patients with < or =200 psoralen+ultraviolet A treatments and more than half of

282 sk was noted during the first 15 years after psoralen+ultraviolet A was discontinued.

283 Psoralen+ultraviolet A-treated psoriasis patients are at

284 ort of 1,380 psoriasis patients treated with psoralen+ultraviolet A.

285 of which 59.4% were considered years without psoralen+ultraviolet.

286 gulation of CYP2S1 by ultraviolet radiation, psoralen-ultraviolet A (PUVA), and topical drugs used to

287 nventional one-photon excitation, as used in psoralen + UV A radiation (320-400 nm) therapy.

288 In this study, using a psoralen-UV cross-linking method and an in vitro RdRP as

289 ral replication by attenuating the VVVs with psoralen-UV light treatment.

290 Electron microscopy of psoralen/UV cross-linked DNA revealed t-loops in enriche

291 Psoralen/UV photocrosslinking studies revealed that meta

292 Similar data were obtained using psoralen/UVA, signifying that MLH1 influences the cellul

293 ous times after introduction into cells, the psoralen was activated by exposure to long wave ultravio

294 was shown to establish the position at which psoralen was added to the target DNA duplex and determin

295 Psoralen was conjugated to the 5'-end of these clamp-for

296 Gene activation by pso-TFOs in which the psoralen was linked to the TFO via a disulfide bond was

297 The activity of this TFO, linked to psoralen, was measured in a mammalian cell line that was

298 f the 5-propynyl-U change the orientation of psoralen with respect to the upper-strand C.

299 clude topical corticosteroids, phototherapy (psoralen with UVA or UVB), topical chemotherapy, topical

300 o attract TnsC, we show that the location of psoralen within the pyrimidine motif triplex does alter

301 roblasts were incubated with tritium-labeled psoralen without TFO to maximize detectable damage and i