ライフサイエンスコーパス: pull-down assay

1 tation and a glutathione S-transferase (GST) pull-down assay.

2 east two-hybrid screening and in an in vitro pull-down assay.

3 on and shown to be 3'UTR-dependent by biotin pull-down assay.

4 GTP) was quantitated by p21-activated kinase pull-down assay.

5 Rho activation was determined using a pull-down assay.

6 PDAC cells from mice and humans using a Raf pull-down assay.

7 E1 initiating enzyme, UBE1L, in an in vitro pull-down assay.

8 action between myosin VIIa and PDZD7 by FLAG pull-down assay.

9 Activation of Cdc42 was determined by GTP pull-down assay.

10 large subunit with PfR4 was detected by GST pull-down assay.

11 s interaction was confirmed in vitro using a pull-down assay.

12 2 and RAC3 using a glutathione S-transferase pull-down assay.

13 rmation of their interaction by the in vitro pull-down assay.

14 ian two-hybrid and glutathione S-transferase-pull down assays.

15 ers advantages over lateral flow or magnetic pull-down assays.

16 ed FRET reporters, or by the use of Rap1-GTP pull-down assays.

17 ited complex using affinity-tagged 3'BTE RNA pull-down assays.

18 1 integrin was demonstrated through in vitro pull-down assays.

19 eting domain (residues 81-230) of Rabex-5 in pull-down assays.

20 l80 that functions in conventional ELISA and pull-down assays.

21 cosedimentation, coimmunoprecipitation, and pull-down assays.

22 o-hybrid and glutathione S-transferase (GST) pull-down assays.

23 .1R was borne out by the results of in vitro pull-down assays.

24 tudy using a yeast two-hybrid screen and GST pull-down assays.

25 icroRNA 195 (miRNA195) was determined by RNA pull-down assays.

26 from mammalian cell lysates and in vitro by pull-down assays.

27 rain also interacts with these same sites in pull-down assays.

28 further confirmed by immunoprecipitation and pull-down assays.

29 ed for interaction with GST or GST-Aalpha in pull-down assays.

30 and tested for binding to GST-Galpha(12) in pull-down assays.

31 llular level of active Rab5-GTP, as shown by pull-down assays.

32 binds directly in glutathione-S-transferase pull-down assays.

33 ced aggregates of Glu1 based on results from pull-down assays.

34 precipitation, biotinylated CaM overlay, and pull-down assays.

35 artners for Ggamma13 by yeast two-hybrid and pull-down assays.

36 act in vitro using glutathione S-transferase pull-down assays.

37 ts interactions with PSD95 in two-hybrid and pull-down assays.

38 nd to coelute by gel-filtration analysis and pull-down assays.

39 l inhibition in vitro through use of protein pull-down assays.

40 in Far-Western analysis and to native IFs in pull-down assays.

41 OCK activity; GTP-bound RhoA was measured in pull-down assays.

42 demonstrated in vitro by GST fusion protein pull-down assays.

43 c peptidoglycan was confirmed by biochemical pull-down assays.

44 demonstrated using yeast two-hybrid and GST pull-down assays.

45 beta with the PRF signal was demonstrated in pull-down assays.

46 rectly to HER2 by co-immunoprecipitation and pull-down assays.

47 were determined by glutathione S-transferase pull-down assays.

48 using colocalization, yeast two-hybrid, and pull-down assays.

49 lular domain directly interacted with Dvl in pull-down assays.

50 rved an interaction between SERT and PIP2 in pull-down assays.

51 provide an effective system that complements pull-down assays.

52 o (co-immunoprecipitation) and in vitro (GST pull-down) assays.

53 al two-hybrid system and in a poly-histidine pull-down assay; 2) immunoprecipitation of the D(2) rece

54 Using a pull-down assay, active GTP-bound rap1 was up-regulated

55 Reporter assay, glutathione-S-transferase pull-down assay, adenovirus-mediated gene transduction,

56 Interestingly, ERalpha-ERE-DNA pull-down assays also revealed that, upon TSEC treatment

57 By employing a bacterial two hybrid system, pull down assays and surface plasmon resonance (SPR) ana

58 D2 loop in protein associations using a GST pull-down assay and a heterologous coexpression system.

59 BDs avidly bound HIV-1 IN in an in vitro GST pull-down assay and each full-length protein potently st

60 The GST pull-down assay and ELISA assay show that Cdc37 binds to

61 Using an SG pull-down assay and mass spectrometry, we found that myo

62 ell as nuclear proteins in mouse brain using pull-down assay and matrix-assisted laser desorption ion

63 ns heterodimerize in vitro and in vivo using pull-down assays and a Forster energy-transfer approach,

64 Pull-down assays and bimolecular fluorescence complement

65 ed by coimmunoprecipitation and in vitro GST pull-down assays and by the identification of a beta-TrC

66 found that they disrupt complex formation in pull-down assays and cellular co-localization studies.

67 h N-ethylmaleimide-sensitive factor (NSF) in pull-down assays and co-immunoprecipitated with NSF in r

68 A16 and IFT46 was confirmed through in vitro pull-down assays and coimmunoprecipitation from flagella

69 eins with Ca(2+)/CaM were confirmed by using pull-down assays and coimmunoprecipitation.

70 ed the binding of SLIP1 to DBP5 and eIF3g by pull-down assays and determined the 3.25 A resolution st

71 Pull-down assays and fluorescence imaging approaches rev

72 1, or both, binding studies utilizing biotin pull-down assays and heterologous luciferase reporter co

73 Further studies with in vitro protein pull-down assays and immunoprecipitation analyses indica

74 and GSK3 was also confirmed by in vitro GST pull-down assays and in situ coimmunoprecipitation assay

75 by glutathione S-transferase fusion protein pull-down assays and in vivo as shown by co-immunoprecip

76 confirmed in vitro by mobility shift and DNA pull-down assays and in vivo by chromatin immunoprecipit

77 ion using co-immunoprecipitation, His-tagged pull-down assays and intracellular immunofluorescence co

78 Using a combination of pull-down assays and isothermal calorimetry, we demonstr

79 ns sensitive to F32L and/or Y96L mutation by pull-down assays and mass spectrometry.

80 hosphatases SHP-1 and SHP-2, as seen in both pull-down assays and microscopy.

81 Glutathione transferase pull-down assays and site-directed mutagenesis revealed

82 By using nucleotide-free RhoG pull-down assays and small interfering RNA-mediated knoc

83 and protocadherin 15 CD3 was confirmed with pull-down assays and surface plasmon resonance analysis,

84 4A in the yeast two-hybrid system and in GST pull-down assays and that the two proteins can be coimmu

85 Using classical pull-down assays and the Hsp90 inhibitory agents geldana

86 of Golgi compaction and vesicle transport in pull-down assays and was required to reconstitute Golgi

87 ed with SPY in yeast two-hybrid and in vitro pull-down assays and were O-GlcNAc modified in Escherich

88 Using pull-down assays and Western blots, we show that TFPI-2

89 ere compared by substrate competition assay, pull-down assay, and surface plasmon resonance (SPR).

90 ePRK1 and LePRK2 in yeast and in an in vitro pull-down assay, and with LePRK2 in a co-immunoprecipita

91 terminus was further substantiated using GST pull-down assays, and binding of the full-length tagged

92 with MPK6 in yeast two-hybrid tests, immuno-pull-down assays, and by bimolecular fluorescence comple

93 We confirmed the interaction by in vitro pull-down assays, and demonstrated that BIF2 phosphoryla

94 ed using phage display, immunoprecipitation, pull-down assays, and gel retardation analysis.

95 pecifically interacts with purified TFIID in pull-down assays, and we have mapped the domains of Rap1

96 120) mutation did not affect DiaA binding in pull-down assays, and we propose that domain II, like Di

97 rotein-interaction domains: most frequently, pull-down assays are used to examine these.

98 Using a pull-down assay based on mixed disulfide fishing, we cha

99 We performed glutathione S-transferase (GST)-pull-down assays between purified KCNQ2-4 carboxy termin

100 They used pull-down assays both in vitro and in vivo to confirm th

101 The interaction was confirmed by a pull-down assay, coimmunoprecipitation, and colocalizati

102 chromatin immunoprecipitation, and in vitro pull-down assays corroborated that Prx1 interacts with A

103 To address this, we used biotin-DNA pull-down assays coupled with mass spectrometry-immunobl

104 Through a combination of pull-down assays, deletion analysis, and isothermal titr

105 SNAP-GST pull-down assays demonstrate SCRIB binds multiple alpha(

106 RNA pull-down assays demonstrate that mRNA containing uridin

107 Single-molecule pull-down assays demonstrate that ORCA-ORC (Origin Recog

108 Further, in vitro pull-down assay demonstrated interactions between gp17 a

109 In vitro pull-down assays demonstrated direct interactions betwee

110 Pull-down assays demonstrated that 14-3-3 proteins assoc

111 Affinity pull-down assays demonstrated that Cry1Fa does not bind

112 tor, Galpha12/13 knockdown and activated Rho-pull-down assays demonstrated that FTY720-P potently act

113 GST pull-down assays demonstrated that Jmjd1a bound all 3 my

114 ChIP and GST pull-down assays demonstrated that liganded VDR blocked

115 iated protein, and glutathione S-transferase pull-down assays demonstrated that MVP1 interacted speci

116 lex immunoprecipitation and biotinylated RNA pull-down assays demonstrated that PARP-14 forms a compl

117 Furthermore, pull-down assays demonstrated that Rrp47p interacts dire

118 Glutathione S-transferase pull-down assays demonstrated that TDG binds to a region

119 Pull-down assays demonstrated that TFII-I interacts with

120 Furthermore, Immunoprecipitation and GST pull-down assays demonstrated that TRIM28 interacts with

121 Bacterial two-hybrid analysis and protein pull-down assays demonstrated the ability of Bsp22 to as

122 y Western blot and glutathione S-transferase pull-down assays demonstrated the association of Tat wit

123 vitro and in vivo glutathione S-transferase pull-down assays demonstrated the two putative glycosylt

124 In vitro pull-down assays demonstrated UreG binding to UreE, with

125 Subsequently, pull-down assays directed at helix alphaC of Cdk2 are sh

126 ion substrates of KEG in vitro, and in vitro pull-down assays document their direct interaction.

127 ing, immunoprecipitation, and an ATP-agarose pull-down assay, EGCG was found to directly modulate the

128 Spectroscopic and pull-down assays fail to detect direct interactions betw

129 tween HIV Gag and human LysRS using affinity pull-down assays, fluorescence anisotropy measurements a

130 Using multiple methods, including pull-down assays, fluorescence polarization, hydrogen/de

131 We used an RNA pull-down assay followed by mass spectrometry and identi

132 CEC) line, and primary HCECs was assessed by pull-down assay followed by Western blotting.

133 SD95 in the cellular milieu was confirmed in pull-down assays following heterologous expression in HE

134 A DNA pull-down assay for proteins interacting with the vieSAB

135 eporter assays and glutathione S-transferase pull-down assays for effector binding.

136 usion constructs of WT and mutant RDS in GST pull-down assays from WT mouse retinal extract.

137 In brain pull-down assays, full-length RGS14 and R14-RBD/GL (but

138 Oligonucleotide pull-down assays further established the capacity of MYC

139 GST pull-down assays further identified three cytosolic doma

140 GST pull-down assays further supported an interaction betwee

141 confirmed by glutathione S-transferase (GST) pull-down assays (GST-NTRPC3 pulled down TRPC1 and vice

142 wn that in a glutathione S-transferase (GST) pull-down assay, GST/hnRNP C1 binds to poliovirus polype

143 In pull-down assays, GST-GEC1 interacted directly with the

144 A pull-down assay identified GAPDH and heat-shock protein

145 Both a yeast two-hybrid screen and a pull-down assay identified plastid ribosomal protein L15

146 Protein domain arrays and pull-down assays identified acetyl "reader" proteins tha

147 firmed that the 2AI is cell permeable, while pull-down assays identified BfmR, a response regulator t

148 Biotinylated-peptide pull-down assays identified DSGIS(338) and DSAPGS(378) a

149 Most importantly, glutathione S-transferase pull-down assays identified that Stat3 binds to the p65

150 Pull-down assays identify at least one putative Ran:L(M)

151 In addition, using pull down assays, immunofluorescence, cell transfection,

152 oresis (PAGE) gel shifts as well as affinity pull-down assays implicated Zu5 and beta-spectrin repeat

153 d for application in the design of selective pull-down assays in proteomics, drug delivery, and nanos

154 Both far-western analyses and immuno-pull-down assays indicate interaction of APE1 with karyo

155 Streptavidin-bead pull-down assay indicated strong binding of biotin-label

156 Reciprocal immunoprecipitation and pull-down assays indicated an interaction between SBDCP1

157 ping studies using glutathione S-transferase pull-down assays indicated that amino acids 137 to 181 o

158 Protein pull-down assays indicated that Arabidopsis FD proteins

159 Protein pull-down assays indicated that Atf4 directly interacts

160 Significantly, our pull-down assays indicated that beta-TrCP binds a phosph

161 Using mRNA arrays, Argonaute pull-down assays, luciferase expression assays, and muta

162 In pull-down assays, MAT2B directly promoted binding of GIT

163 In glutathione S-transferase pull-down assays, MBD3L2 is found associated with severa

164 Using yeast two-hybrid screening and pull-down assays, MDT-28/PLIN-1 was found to bind to DLC

165 on of CcpA-promoter interactions using a DNA pull-down assay mimicking physiological conditions showe

166 In yeast two-hybrid and pull-down assays, MORF proteins can connect to form hete

167 t bind ENaC in cells, as assessed by in vivo pull-down assays, nor did it phosphorylate ENaC in vitro

168 Pull-down assays, nuclear magnetic resonance, and isothe

169 Pull-down assays of pulse-labeled mitochondria enabled u

170 x-containing telomerase RNA was confirmed by pull-down assays of the endogenous RNAs.

171 native claudin-4 on transfected CHO cells in pull-down assays, only the larger Cpe30 peptide affected

172 Using GST pull-down assays, PCNA-coupled affinity beads pull-down

173 In pull-down assays, peptides containing CvpA-sorting motif

174 F4G binding, it binds m(7)GTP weakly, and in pull-down assays, rather than binding eIF4G, it binds 4E

175 molecular fluorescence complementation and a pull-down assay, respectively.

176 oprecipitation and glutathione S-transferase pull-down assay results indicated that caveolin-1 and BK

177 Pull-down assays reveal that Liprin-alpha interacts with

178 scence results and glutathione S-transferase pull-down assays revealed an association of Ndm with cor

179 In vivo pull-down assays revealed Asp80 as critical for stabiliz

180 atin immunoprecipitation and oligonucleotide pull-down assays revealed ERalpha binding to the PBF pro

181 Both in vitro and ex vivo pull-down assays revealed that equol directly bound with

182 Pull-down assays revealed that GATA-3 could bind oligonu

183 Pull-down assays revealed that hyperacetylated p300 HAT

184 Pull-down assays revealed that Rb interacts with active

185 Pull-down assays revealed that RWE or quercetin (but not

186 on experiments and glutathione-S-transferase pull-down assays revealed that TLX1 directly binds to CB

187 ybrid library screening and in vitro protein pull-down assays revealed that XLG2 interacts with the n

188 binding site, confirmed via oligonucleotide pull-down assays, revealed increased HAB promoter activi

189 As shown in glutathione S-transferase pull-down assays, S141A bound to Cdc42(GTP) at a 6-fold

190 In vitro pull-down assays show an interaction between monomers th

191 s of ND7 neuroblastoma cells, while in vitro pull-down assays show direct association.

192 triphosphate-agarose and calmodulin-agarose pull-down assays show that the TRPV6-ARD does not intera

193 Docking model and pull down assay showed that PL directly binds to the DNA

194 on experiments and glutathione S-transferase pull-down assay showed a direct interaction between RARb

195 A biotin-streptavidin pull-down assay showed that 4,11-bis(2-aminoethylamino)a

196 A DNA pull-down assay showed that the -401 TIE was capable of

197 Western immunoblot and microaffinity DNA pull-down assays showed a parallel increase in nuclear t

198 atin immunoprecipitation and oligonucleotide pull-down assays showed that both FOXO3a and FOXM1 bind

199 In vitro pull-down assays showed that both receptors can interact

200 Pull-down assays showed that both wild-type and ZASP1-D1

201 ng with phospho-specific antibodies, and GST pull-down assays showed that Nck determines spatiotempor

202 Mammalian two-hybrid and GST pull-down assays showed that PK11195 repressed the inter

203 Pull-down assays showed that the AQP9 COOH-terminal SVIM

204 Finally, GST pull-down assays showed that the LW motif is part of a p

205 Glutathione-S-transferase pull-down assays showed that the MZF1 SCAN domain self-a

206 Two-hybrid and pull-down assays showed that these two proteins interact

207 Glutathione S-transferase pull-down assays showed the selective interaction of alp

208 me of which indeed interact with aldolase in pull-down assays, suggest supplementary, non-glycolytic

209 A2 in coimmunoprecipitation and in vitro GST pull-down assays, suggesting that regulation involved di

210 The pull-down assay suggests an association of the N-termina

211 In glutathione S-transferase pull-down assays, TDP-43 bound to karyopherin-alphas, th

212 dy, we show by coimmunoprecipitation and GST pull-down assays that BK(Ca) channels can associate with

213 We show by GST-LFn pull-down assays that LFn specifically interacts with at

214 brid assay and the glutathione S-transferase pull-down assay, that mouse REV1 can physically interact

215 In a pull-down assay, the amount of Arp1 pulled down with its

216 Using a RIC-3 affinity pull-down assay, the interaction between MBP-5HT(3A)-ICD

217 athways) has been demonstrated using the GST pull-down assay, the yeast two-hybrid assay, as well as

218 By GST pull-down assays, the ligand-binding domain of TR was id

219 Mass-spectrometry (MS) subsequent to a ChIP pull-down assay to characterise the components of the pr

220 reporter probes in a single-molecule protein pull-down assay to characterize antibody binding and tar

221 We developed an effector pull-down assay to test this model.

222 ce spectroscopy and conventional biochemical pull-down assays to demonstrate a direct interaction bet

223 they were used in glutathione S-transferase pull-down assays to determine their binding properties.

224 These two proteins were used in pull-down assays to identify interacting PPD products.

225 export assays, and glutathione S-transferase pull-down assays to investigate the export pathway used

226 through the small GTP-ase, Rac1 and we used pull-down assays to investigate the role of Eps8 in Rac1

227 ar fluorescence complementation and in vitro pull-down assays to investigate the supramolecular organ

228 f the compound library and performed protein pull-down assays to purify the anticancer targets of the

229 In a pull-down assay, uracil DNA glycosylase 2 (UNG2), an imp

230 Results from pull-down assay using 7-methyl-GTP Sepharose 4B beads in

231 A pull-down assay using biotin-labeled S100B was used to d

232 A pull-down assay using biotinylated siRNA- or DNA-conjuga

233 A pull-down assay using cytosolic proteins identified that

234 hibition of SP-A binding to agarose beads, a pull-down assay using His-tagged Prdx6 and Ni(2) -chelat

235 A pull-down assay using nuclear proteins demonstrated that

236 Glutathione S-transferase (GST) pull-down assay using recombinant GST-occludin demonstra

237 tion and verified by confocal microscopy and pull-down assay using recombinant or in vitro translated

238 A pull-down assay using the T. cruzi component of the prer

239 by means of glutathione S-transferase (GST) pull-down assays using GST fused to the Ran binding doma

240 ERalpha)-estrogen response element (ERE)-DNA pull-down assays using HeLa nuclear extracts followed by

241 GST-AE1 pull-down assays using human kidney membrane proteins sh

242 endent as shown in glutathione S-transferase pull-down assays using native and recombinant Cpn0585.

243 Pull-down assays using nuclear extracts from osteoblasts

244 In contrast, pull-down assays using purified glutathione S-transferas

245 HTLV-1-infected T cells (C81) as well as GST pull-down assays using purified proteins.

246 Results of in vitro pull-down assays using recombinant proteins confirmed th

247 nteraction between Golgin45 and Syntaxin5 in pull-down assays using recombinant proteins, whereas thi

248 Pull-down assays using recombinant-purified cortactin an

249 These were further confirmed by peptide pull-down assays using specific mutations in the interac

250 In vitro pull-down assays using truncated constructs have also re

251 In vitro pull-down assays verified binding between maltose-bindin

252 The GST)-effector pull down assay was used to study the presence of active

253 A GTP pull-down assay was performed to identify Rac1-GTP.

254 Here, the glutathione transferase pull-down assay was used to investigate interaction betw

255 Using an affinity pull-down assay we identified the potassium transporter-

256 Furthermore, by GST pull-down assay we observed that PC1L2 and polycystin-1L

257 covery after photobleaching, and biochemical pull down assays, we demonstrated that recycling of inte

258 Using NMR and reciprocal pull down assays, we present the first direct evidence t

259 Using an in vitro pull-down assay, we demonstrate that the interaction bet

260 Using glutathione S-transferase (GST) pull-down assay, we demonstrated that the intracellular

261 Using a membrane-based pull-down assay, we have identified a binding "hot-spot"

262 Using a pull-down assay, we show interaction between caspase 6 a

263 nce resonance energy transfer technology and pull-down assays, we confirmed that these two subunits i

264 rmatics analysis, coimmunoprecipitation, and pull-down assays, we discovered a novel, direct binding

265 oprecipitation and glutathione S-transferase pull-down assays, we discovered and confirmed that Saito

266 oinformatic analyses, as well as RIP and RNA pull-down assays, we discovered and validated that the l

267 Using glutathione S-transferase (GST) pull-down assays, we found that the ESEV PBM enables NS1

268 Using in vivo pull-down assays, we have determined that the C-terminal

269 By coimmunoprecipitation and pull-down assays, we provide evidence that AtPDCD5 inter

270 Using pull-down assays, we show that an effector loop, the U6

271 Using yeast two-hybrid and GST-pull-down assays, we show that ovulin interacts with its

272 ctive labeling on Hrp1, in vivo activity and pull-down assays, we were able to study this complex of

273 PTP-PEST interaction with SKAP-Hom, in vitro pull down assays were performed demonstrating that the P

274 d as VPg, therefore yeast two-hybrid and GST pull-down assays were carried out with proteins encoded

275 GTP pull-down assays were performed to identify the activate

276 Pull-down assays were used to demonstrate increased acti

277 Glutathione S-transferase pull-down assays were used to examine whether DTACs alte

278 mechanisms of OsHAD1, we performed in vitro pull-down assays, which revealed the association of OsHA

279 Experiments using a pull-down assay with [3H]EGCG demonstrate binding of EGC

280 leavage was also demonstrated in an in vitro pull-down assay with agarose bead-immobilized AtS1P.

281 A protein pull-down assay with biotinylated glutathione ethyl este

282 Here, we used a direct toxin pull-down assay with immobilised KcsA potassium channel

283 ligation assay, co-immunoprecipitation, and pull-down assay with recombinant extracellular CHL1 doma

284 transcription factor C/EBPalpha by use of a pull-down assay with S-tagged C/EBPalpha combined with m

285 at combines the principles of a conventional pull-down assay with single-molecule fluorescence micros

286 The assay combines the conventional pull-down assay with single-molecule total internal refl

287 Pull-down assay with the p53-specific antibody has shown

288 stent with S-glutathionylation, streptavidin pull-down assays with biotinylated glutathione ethyl est

289 recipitation from mouse brain lysates and by pull-down assays with exogenously expressed proteins.

290 Pull-down assays with full-length and truncated Ssa2 pro

291 Pull-down assays with glutathione S-transferase-FliM sho

292 Pull-down assays with GST (glutathione S-transferase) pr

293 Protein pull-down assays with GST-IE62 fusion proteins containin

294 mass spectrometry on proteins obtained from pull-down assays with GST-nephrin cytoplasmic domain.

295 Using affinity pull-down assays with immunoblotting and immunofluoresce

296 In pull-down assays with lectins, we found a notable differ

297 Cdc42 activation was determined by pull-down assays with PAK-agarose beads.

298 Pull-down assays with recombinant UBR proteins suggest t

299 FA pull-down assays with Western blot analysis revealed a F

300 In pull-down assays, ZAP bound to viral RNA transcripts wit