ライフサイエンスコーパス: pulse label

1 decay after experimental intervention (e.g., pulse labeling).

2 he greatest rate (approximately 90 min after pulse labeling).

3 ction was characterized by hydrogen exchange pulse labeling.

4 ediate was studied by hydrogen exchange (HX) pulse labeling.

5 in the far-UV region and by NMR quench-flow pulse labeling.

6 to levels that were detectable other than by pulse labeling.

7 -UTP and FITC-conjugated CTP within 5 min of pulse labeling.

8 gical volumetry and bromodeoxyuridine (BrdU) pulse labeling.

9 d peripheral T cell proliferation by genetic pulse labeling.

10 early folding events has been analyzed by pH-pulse labeling.

11 al DNA synthesis by bromodeoxyuridine (BrdU) pulse-labeling.

12 of 5-bromo-2-deoxyuridine following in vivo pulse-labeling.

13 ne H3 immunoreactivity and bromodeoxyuridine pulse labelling.

14 mide only under chase labeling but not acute pulse labeling, 3) the induction of the levels of sphing

15 under the conditions used for the [(32)P]GTP pulse labeling, (32)P was incorporated into the entire m

16 Assays for pulse-labeled [3H]DNA and for total DNA indicated that t

17 produce full-length Lhcb as demonstrated by pulse-labeling: a new radioactively labeled band of a si

18 In the absence of substantial SecA, pulse-labeled AcrB was retained in the cytoplasm even af

19 Plants were pulse-labelled after the drought with (13) C-CO(2) to qu

20 DcxCreER(T2) transgenic mice to permanently pulse-label age-defined cohorts of granule cells born ei

21 Pulse-labeling alkaline elution showed deficiency of dam

22 The (13)C pulse labeling allowed tracing the C of switchgrass orig

23 Pulse-labeling analyses show that formation of prenyl-pA

24 In this study using ex vivo pulse label analysis, we demonstrate that AIPL1 is not i

25 Pulse-labeling analysis disclosed a time-dependent reduc

26 Pulse-labeling analysis indicated that Nodals and Leftys

27 ll kinetic folding, the present work used HX pulse labeling analyzed by a fragment separation-mass sp

28 After a 30-min [35S]methioneine/cysteine pulse labeling and 120 min of chase, all of the nascent

29 Pulse labeling and chase of mitochondrial translation pr

30 he nonprocessed D1 precursor was observed by pulse labeling and immunodetection in LAHG-grown PS I-le

31 H24L/H119F were studied by hydrogen exchange pulse labeling and interrupted hydrogen/deuterium exchan

32 ompared at high resolution by quench-flow pH-pulse labeling and interrupted hydrogen/deuterium exchan

33 In this study, we employed a combined pulse labeling and neutral-neutral two-dimensional gel-b

34 lts from kinetic deuterium hydrogen exchange pulse labeling and protein engineering studies.

35 In organello, pulse labelling and pulse-chase experiments have enabled

36 Pulse labelling and treatment of cells with the translat

37 Pulse-label and pulse-chase experiments show that GPA is

38 that had not been looked at previously were pulse-labeled and analyzed for the presence of newly tra

39 r membrane surface proteins were vectorially pulse-labeled and flagella and vesicles were analyzed fo

40 half-lives than wild-type controls for total pulse-labeled and individual mRNAs.

41 change using 5-bromo-2'-deoxyuridine (BrdU) pulse-labeling and DAPI (4',6-diamidino-2-phenylindole)

42 ons, Western blot (immunoblot) analysis, and pulse-labeling and immunoprecipitation of both fusion pr

43 We report here the results of pulse-labeling and immunoprecipitation studies of this r

44 Pulse-labeling and metabolic chase analysis suggested th

45 is increased by ppGpp as judged by both RpoS pulse-labeling and promoter-independent effects on lacZ

46 en using substituted pyrimidine analogues in pulse-labeling and suggest that EdU is the preferable nu

47 progression, we followed CHH fibroblasts by pulse-labeling and time-lapse microscopy.

48 Immunoblot, pulse labeling, and ribosome profiling analyses of mutan

49 during superinduction, yet Western blotting, pulse labeling, and the use of bicistronic vectors showe

50 ransfer activity was inhibited at the end of pulse labeling, apoB100 secretion was abolished.

51 producible mass spectrometry-based method, a pulse labeling approach using stable isotope-labeled ami

52 Here, we use a pulse-labeling approach with a monovalent anti-Env Fab p

53 was verified using an in vivo stable isotope pulse-labeling approach, and their exact ribosomal prote

54 onstraining growth conditions in radioactive pulse-labeling approaches.

55 ished proinsulin biosynthesis, observed in a pulse-labeling as short as 5 minutes.

56 uses is polyadenylated like bacterial mRNAs, pulse-labelled as well as the steady-state population of

57 Using a dye (PKH26) to pulse label ATMs in vivo, we purified macrophages recrui

58 siae using a combination of quantitative and pulse labeling-based proteomics approaches, in vitro stu

59 Approximately 25% of all pulse-labeled beta-catenin destined for E-cadherin assoc

60 We found that pulse-labeled beta-catenin replaces the beta-catenin bou

61 y inhibit the assembly of apoB-100 VLDL) and pulse-labeled (+BFA) and chased (+BFA) for 30 min to obt

62 ional viral RC, detected by incorporation of pulse-labeled bromodeoxyuridine into newly synthesized D

63 on in HCMV-infected cells, they incorporated pulse-labeled bromodeoxyuridine, and their formation was

64 oteins for anterograde axonal transport were pulse labeled by intravitreous injection of (35)S-methio

65 e that reporter protein could be efficiently pulse-labeled by soaking animals in ligand.

66 , which accumulated in the medium, even from pulse labeled cells, was predominantly in the high molec

67 association of MTP and apoB, as assessed in pulse-labeled cells by co-immunoprecipitation, was trans

68 In pulse label-chase experiments, [Cl-] was 19 mM just afte

69 In the present study, we pulse-labeled chick embryos by injecting low doses of th

70 or 14 days after crushing the sciatic nerve; pulse-labeled class II and class III beta-tubulin were i

71 pped-flow fluorescence and hydrogen exchange pulse labeling coupled with mass spectrometry.

72 n T-87 cell lipid assembly were evaluated by pulse labeling cultures with [(14)C]acetate and [(14)C]g

73 ication and endoreduplication in nuclei from pulse-labeled developing maize root tips.

74 Amino acid pulse labeling directly establishes that much of the ste

75 iofilms using immunofluorescent detection of pulse-labeled DNA and also an inducible green fluorescen

76 Furthermore, bromodeoxyuridine (BrdU)-pulse-labeled DNA synthesis initiation sites colocalized

77 Nevertheless, replication foci pulse labeled during any short interval of S phase were

78 Previous pulse labeling efforts have always assumed EX2 exchange

79 f the two UDP-sugar substrates separately to pulse label either the beginning or the end of HA chains

80 5 days in labeled medium and also in a 1-day pulse labeling experiment where the washout of label was

81 Here, we revisited the pulse labeling experiment with barnase and detected no s

82 tudies, an amide hydrogen/deuterium exchange pulse-labeling experiment detected a stable submilliseco

83 on of a double-jump experiment followed by a pulse-labeling experiment.

84 e novo sphingolipid pathway as determined by pulse labeling experiments and inhibition studies with m

85 Pulse labeling experiments demonstrated that newly synth

86 nt for NMR measurements after quench-flow pH-pulse labeling experiments gives a greatly increased dat

87 Pulse labeling experiments indicate that int6Delta signi

88 Pulse labeling experiments indicate that LS is synthesiz

89 Hydrogen exchange pulse labeling experiments indicate that, in contrast to

90 Pulse labeling experiments reveal that, in immature cons

91 Pulse labeling experiments revealed that rates of protei

92 tion did not alter PIKK mRNA levels, in vivo pulse labeling experiments showed that Tel2 controls the

93 Furthermore, [(32)P(i)] pulse labeling experiments uncovered alterations in phos

94 In pulse labeling experiments, AAI protected TraR against p

95 labeling by amino acids in cell culture and pulse labeling experiments.

96 Finally, pulse labelling experiments demonstrated that metabolic

97 In contrast, pulse labelling experiments of GDF-5-infected limbs show

98 Moreover, yeast pulse-labeling experiments argue against there being a s

99 s observed in the previous hydrogen exchange pulse-labeling experiments at pH 6.2 and 10 degrees C.

100 Consistent with this, pulse-labeling experiments demonstrate a significant red

101 [35S]Methionine pulse-labeling experiments demonstrated that GIRK4 assoc

102 uch that the results of our model agree with pulse-labeling experiments from three different nerve ce

103 Strikingly, pulse-labeling experiments indicate that total poly(A)(+

104 Pulse-labeling experiments indicate that ubiquilin facil

105 BrdU pulse-labeling experiments revealed that virtually all s

106 Hydrogen exchange pulse-labeling experiments show that the slow-folding ph

107 Pulse-labeling experiments showed that expression of at

108 Pulse-labeling experiments showed that in vivo CL biosyn

109 Pulse-labeling experiments showed that most major late p

110 Pulse-labeling experiments showed that newly synthesized

111 5-Iododeoxyuridine/5-chlorodeoxyuridine pulse-labeling experiments showed that RAD6 is necessary

112 In pulse-labeling experiments that followed nascent MYOC ov

113 anism, and we perform genetic decoupling and pulse-labeling experiments to demonstrate that Sis1 indu

114 Pulse-labeling experiments using 5-bromo-2-deoxyuridine

115 Results of pulse-labeling experiments with [35S]methionine further

116 the preferable nucleoside analogue for short pulse-labeling experiments, resulting in increased recov

117 Hydrogen exchange pulse-labeling experiments, with NMR detection, were per

118 lly induced dynamic nuclear polarization NMR pulse-labelling experiments that involve rapid in situ p

119 rogen deuterium exchange (HDX) data from NMR pulsed labelling experiments, and uses backbone and side

120 d a severe reduction in the incorporation of pulse-labelled flagellin into the membrane/flagellum fra

121 Hydrogen exchange pulse-labeling followed by mass spectrometry reveals det

122 hin a cell population can be demonstrated by pulse-labeling followed by PCR amplification of immunopr

123 When HSP16.9 is pulse labeled for 10 ms, residues 1-40 and 131-151 are s

124 First, McA RH7777 cells were pulse-labeled for 20 min with [35S]methionine/cysteine a

125 When pulse-labeled Gag precursors from High Five cells were f

126 alphaherpesvirus gI homologs, a fraction of pulse-labeled gI synthesized in BHV-1-infected cells app

127 Pulse-labeling HDX-MS studies revealed that ANGPTL3/8 an

128 d to establish the structure of the PUFs and pulse-labelled HDX NMR was used to show that the PUFs an

129 l phosphates on prothymosin alpha in vivo by pulse-labeling HeLa cells with [32P]orthophosphate and c

130 , and also the recently introduced transient pulse-labeling HX experiments.

131 using circular dichroism, fluorescence, and pulse-labeling hydrogen exchange.

132 Pulse-labeling hydrogen-deuterium exchange experiments m

133 In pulse-labelled hydrogen/deuterium exchange experiments m

134 data set of early folding residues based on pulsed labeling hydrogen deuterium exchange experiments.

135 ived from aldolase incubated in 3 M urea and pulse labeled in (2)H2O.

136 rongylocentrotus droebachiensis embryos were pulse labeled in the presence of colchicine or taxol at

137 Uridine pulse labeling in intact CS-B fibroblasts and lymphoblas

138 Using pulse labeling in vivo and synchronized translation in v

139 nt transport waves obtained by radioisotopic pulse labeling in vivo.

140 fragments derived from folded cytochrome c, pulse-labeled in the same manner, to indicate the percen

141 Pulse-labeling in the presence of cycloheximide indicate

142 ld experiment, using in situ (13)C and (15)N pulse-labelling in intensively and extensively managed f

143 no presaturation pulse, (2) a presaturation pulse labeling inferior vena cava (IVC) blood (signal vo

144 Interestingly, shortly after pulse labeling INS cells, a substantial fraction of both

145 Pulse-labeling interaction studies reveal that TMEM126A

146 ombination of 5-bromo-2'-deoxyuridine (BrDU) pulse labeling, intracellular biocytin labeling, and imm

147 e molecules into newly synthesized DNA (i.e. pulse-labeling) is used to monitor cell proliferation or

148 egral membrane proteins, indicating that the pulse-labeled Lhcb is readily integrated into the membra

149 resolution by an advanced hydrogen-exchange pulse-labeling mass-spectrometry method (HX MS).

150 Pmel17 immunoprecipitates from metabolically pulse labeled melanoma cells and melanocytes contain, in

151 ring folding and used this to provide an NMR pulse labeling method for determining structures of fold

152 Key developments over time include the HX pulse labeling method with nuclear magnetic resonance an

153 Here, we used a pulse-labeling method in Mus musculus models for trackin

154 ic mice overexpressing NF-M by the classical pulse-labeling method using 35S-methionine.

155 ng" method, which is less accurate than the "pulse-labeling" method typically used in mammals.

156 Pull-down assays of pulse-labeled mitochondria enabled us to characterize Co

157 FE) has been studied using hydrogen exchange pulse labelling monitored by 2D 1H NMR, and by stopped f

158 We also found that the rate at which pulse-labeled monophosphorylated MAPK became bisphosphor

159 More than 50% of pulse-labelled mutant DNA was in short chains characteri

160 In vivo pulse-labeled myosin Va advances along axons at slow tra

161 initiation and elongation, as determined by pulse-labeling nucleotide incorporation in replication f

162 -retaining cells (LRCs) were determined by a pulse label of newborn mice with BrdU, followed by a cha

163 rage of approximately 1,100 RS after a 5-min pulse labeling of 3T3 mouse fibroblast cells in early S-

164 In vivo pulse labeling of an mto1 mutant, however, indicate incr

165 Pulse labeling of ATMs with PKH26 assessed the recruitme

166 thesis by TGF-beta and CTGF was confirmed by pulse labeling of cells with [35S]methionine.

167 Bromodeoxyuridine (BrdU) pulse labeling of cortical slices cultured in NMDA antag

168 Pulse labeling of endothelial cells with cholera toxin B

169 Biosynthetic pulse labeling of five human glycoproteins showed that e

170 In this study, we used stable isotope pulse labeling of human pulmonary artery endothelial cel

171 Western blotting experiments and pulse labeling of infected cells with [(35)S]methionine

172 Pulse labeling of mitochondrial protein synthesis produc

173 lable single-nucleus RNA-Seq (sNuc-Seq) with pulse labeling of proliferating cells by 5-ethynyl-2'-de

174 Immediately after UV irradiation and a short pulse labeling of repair patches, intact nuclei were dig

175 n analysis of ATPase transcripts and in vivo pulse labeling of the mitochondrial translation products

176 Pulse labeling of tumor and TDLN T cells with BrdU confi

177 Pulse labelling of CDP-DG and PG, shown previously to in

178 However, pulse labelling of PS, which normally increases during i

179 Subcellular fractionation after pulse labelling of the cells with 125I-ABL for 2 h at 4

180 Pulse-labeling of cell wall glucans indicated wall synth

181 he most common nucleoside analogues used for pulse-labeling of DNA in cells are the deoxypyrimidine a

182 Pulse-labeling of DNA with EdU and RNA with BrU and test

183 Pulse-labeling of infected cells revealed that LEF-12 mu

184 Pulse-labeling of lpt1Delta strains showed a 30% reducti

185 Metabolic pulse-labeling of nascent RNA with 4'Thiouridine was use

186 Pulse-labeling of progenitors with bromodeoxyuridine sho

187 tu hybridization analyses to detect mRNA and pulse-labeling of proteins were used to examine several

188 We also note that the pulse-labelling pattern of proteins at 42 C (displayed o

189 cules, the DNA sequences replicated during a pulse-labeling period.

190 hosphatase, whereas in F- cells, < 5% of the pulse-labelled polypeptide was processed.

191 aline phosphatase activities and patterns of pulse-labelled polypeptides indicated that TraA'-'PhoA-1

192 retory granules, ISGs): Inhibiting export of pulse-labeled POMC by brefeldin A (BFA) or a 20 degrees

193 eta-cell lines, we have followed the fate of pulse-labeled procathepsin B (ProB, a lysosomal proenyzm

194 ysis of the peptic peptides derived from the pulse-labeled product of the sub-millisecond folding rea

195 ed by Western blot or immunoprecipitation of pulse-labeled protein.

196 PC precursors were detected among pulse-labeled proteins in transformants with N-terminal

197 Furthermore, analysis of pulse-labeled proteins indicated prolonged synthesis of

198 n viral protein accumulation were studied by pulse-labeling proteins in infected protoplasts.

199 Here we use a pulse-block-pulse labeling protocol with fluorescent ligands to labe

200 Using a novel pulse labeling/quantitative mass spectrometry technique,

201 Here, using high-resolution separation of pulse-labeled replication intermediates coupled with str

202 However, short pulse labeling revealed that the initial translation rat

203 Multipoint BrdU pulse-labeling revealed that, compared to cells actively

204 Moreover, although pulse-labeled RNA decays normally in orn mutant cells un

205 At 44 degrees C, the half-life of total pulse-labeled RNA rose from 2.9 min in a wild-type strai

206 Pulse-labeling RNA studies showed a PARP-dependent incre

207 led to a decrease in the half-life of total pulse-labelled RNA along with decreased half-lives of th

208 We found that about 85% of pulse-labeled rp mRNA was associated with polysomes in e

209 Pulse labeling showed that the rate of recruitment of ne

210 zonal centrifugation.; (ii) fractionation of pulse-labeled steady-state cultures according to cell ag

211 along with a 5-bromo-2'-deoxyuridine (BrdU) pulse-label strategy, we compared memory cells to their

212 We pulse-labeled striosomal cells (S cells) and matrix cell

213 Second, pulse labeling studies demonstrated increased production

214 Pulse labeling studies indicated that newly replicated m

215 PtdIns dramatically in both steady-state and pulse labeling studies, suggesting that the observed eff

216 Immunofluorescence and pulse labelling studies indicate that this is a previous

217 Pulse-labeling studies and immunoblot analyses showed th

218 We have used pulse-labeling studies and the expression of the ARG8(m)

219 m unloading, making it a suitable tracer for pulse-labeling studies of phloem transport.

220 Pulse-labeling studies reveal that extracellular secreti

221 Pulse-labeling studies show that when K(IR)6.2 is expres

222 Results from our pulse-labeling studies showed that Cdc37 protects nascen

223 BrdU, although cell cycle analyses and BrdU pulse-labeling studies suggested that most of this proli

224 d stability of the molecule, as indicated by pulse-labeling studies, demonstrating a prolonged half-l

225 For the G8 mutant, from these assays and pulse-labeling studies, we determined the ratio of synth

226 By performing BrdU pulse-labeling studies, we found that MBC formation prec

227 ntracellular betaPPs and Abeta shortly after pulse labeling suggests that Abeta is produced in the se

228 blood (signal void), and (3) a presaturation pulse labeling superior vena cava (SVC) blood.

229 onses, we used radioactive orthophosphate to pulse-label suspension-cultured cells of Arabidopsis in

230 use footpads using a newly developed in situ pulse labeling technique.

231 Here we used steady-state and pulse-labeling techniques to follow Notch receptors in s

232 the IL-6(-/-) mice show more hepatocyte BrdU pulse labeling than the IL-6(+/+) controls at 24 hours,

233 We pulse-labelled the soil surrounding wheat (Triticum aest

234 Within 10 min of pulse labeling, the mutant protein undergoes a molecular

235 tivities with gp160 at different times after pulse-labeling, the MAbs were sorted into groups that ex

236 xamined by sucrose gradient fractionation of pulse-labeled thylakoids; the accumulation of high-molec

237 We use live imaging and pulse labeling to quantitatively determine the fates of

238 Thus, we were able to use FSPM pulse-labeling to localize PBP4 activity in live cells,

239 We used (13) C pulse-labelling to trace assimilated C in mosses (Sphagn

240 About 70% of the pulse-labelled TraA-'PhoA-121 polypeptide was rapidly pr

241 Pulse-labeled transcription complexes elongated in the p

242 Pulse-labeled transcription complexes established from i

243 intermediate promoter but is observed using pulse-labeled transcription complexes initiated from all

244 We used 5-ethynyluridine to pulse-label transcripts during mitosis and mitotic exit

245 ys, and also strongly influenced the fate of pulse-labeled TraR.

246 ween breeding and nonbreeding conditions, we pulse-labeled two different cohorts of new HVC neurons u

247 In contrast, only 50% of pulse-labeled type I receptor is converted to the Golgi-

248 Vascular SMCs were genetically pulse-labeled using the tamoxifen-dependent Cre recombin

249 Hydrogen exchange pulse labeling was used to establish the structure of th

250 baculoviruses (BV) were plaque purified, and pulse-labeling was used to verify the synthesis and secr

251 hetic rate, measured using [(35)S]methionine pulse labeling, was decreased by 75% in the diabetic adi

252 Release of ABL from the cell, after pulse labelling, was assessed using both fluorescein iso

253 Using SNAP-based fluorescent pulse labeling, we now demonstrate that cell cycle-restr

254 Here, using 13CO2 pulse labeling, we show that natural densities of the nu

255 ng intermediates were probed by H/D exchange pulsed labeling, which showed the coexistence of noncomp

256 e from cytoplasm to chloroplast, Euglena was pulse labeled with 35S-sulfate and the organelles were s

257 maize seedlings at the third-leaf stage were pulse labeled with [(14)C]O(2) and Golgi membranes were

258 Asynchronously cycling cells are pulse labeled with the nucleotide analog 5-bromo-2-deoxy

259 Pulse labeling with 5-EU revealed nascent and unstable R

260 Pulse labeling with [(35)S]methionine and biotinylation

261 The 41-kDa species, emerging within 5 min of pulse labeling with [(35)S]methionine, is converted into

262 y incorporated into camalexin during a 1.5-h pulse labeling with [14C]anthranilate also increased wit

263 incorporation into camalexin during a 1.5-h pulse labeling with [14C]indole was similar to that with

264 TP photoaffinity inhibitor BMS-192951 before pulse labeling with [35S]methionine/cysteine led to an 8

265 Following pulse labeling with [3H]arachidonic acid ([3H]AA), its i

266 Single pulse labeling with [3H]thymidine at 36 h labeled Thy 1-

267 Pulse labeling with [3H]thymidine confirmed in vitro neu

268 Single as well as multiple pulse labeling with [3H]thymidine confirmed that the ent

269 ease H was studied by hydrogen exchange (HX) pulse labeling with analysis by an advanced fragment sep

270 n 17 HIV-infected patients by 30 min in vivo pulse labeling with bromodeoxyuridine (BrdU).

271 x HIV-1-infected patients studied by in vivo pulse labeling with bromodeoxyuridine.

272 Recent work using HX pulse labeling with MS analysis finds that a number of p

273 ucted by genome-wide molecular combing after pulse labeling with two thymidine analogues.

274 was grown in (15)N-labeled soil columns and pulse-labeled with (13)CO(2) to visualize the spatial di

275 s transduced with HPV-18 E7 were pulse-chase-pulse-labeled with (3)H-thymidine ((3)H-TdR) and bromode

276 Moreover, when intact neurons were pulse-labeled with 3H-labeled sugars at low temperature

277 whereby unsynchronized cell populations are pulse-labeled with 5-bromo-2'-deoxyuridine (BrdU), fract

278 newly synthesized (pro)insulin, islets were pulse-labeled with [(3)H]tyrosine (40 microCi) for 20 mi

279 eated with 62.5 microM 6AN for 21 h and then pulse-labeled with [(35)S]methionine for 1 h, increased

280 riton X-100-fractionated extracts from cells pulse-labeled with [(35)S]methionine indicated that HMW1

281 idine residues at its carboxyl terminus were pulse-labeled with [35S]cysteine, and the labeled norrin

282 Human monocyte-derived macrophages were pulse-labeled with [35S]Met and prepared for affinity ch

283 thesis, WI-38 human diploid fibroblasts were pulse-labeled with [35S]methionine, and calpain was immu

284 Frog retinas were pulse-labeled with [35S]methionine/cysteine and [3H]DHA

285 4 and Chinese hamster ovary (CHO) cells were pulse-labeled with BAC-TMR-dextran by fluid-phase endocy

286 Samples withdrawn during dialysis were pulse-labeled with deuterium to identify unfolded region

287 wing dilution from 8 M urea, the protein was pulse-labeled with deuterium, quenched with acid and mas

288 ation of urea, the amide hydrogen atoms were pulse-labeled with deuterium, the labeled samples were q

289 nd late-replicating chromosomal domains were pulse-labeled with halogenated nucleotides and prelabele

290 periods of time with chlorodeoxyuridine and pulse-labeled with iododeoxyuridine 8 h before tumor rem

291 in a high-speed quenched-flow apparatus and pulse-labeled with protium to identify unfolded regions.

292 Proteins were pulse-labeled with radioactive isotope (35S or 14C) in c

293 Pulse-labeling with (15)N-labeled medium time-stamps the

294 in guanidine hydrochloride (GdHCl) or urea, pulse-labeling with 2H2O and analyzing the intact protei

295 lutions of TIM unfolded in GdHCl or urea and pulse-labeling with 2H2O at different times.

296 ing after 24 hours was preceded by 2 hour of pulse-labeling with 5-bromodeoxyuridine.

297 smission of herpes simplex virus (HSV) using pulse-labeling with ethynyl nucleotides and cycloadditio

298 ra, transducin activation, Western blotting, pulse-labeling with immunoprecipitation, and immunocytoc

299 as well as LC-MS/MS approaches incorporating pulse-labelling with stable isotopes (SILAC) to monitor

300 Pulse-labelled YopE, but not YopQ, could be secreted aft