ライフサイエンスコーパス: pulse labeling

1 decay after experimental intervention (e.g., pulse labeling).

2 he greatest rate (approximately 90 min after pulse labeling).

3 in the far-UV region and by NMR quench-flow pulse labeling.

4 to levels that were detectable other than by pulse labeling.

5 -UTP and FITC-conjugated CTP within 5 min of pulse labeling.

6 gical volumetry and bromodeoxyuridine (BrdU) pulse labeling.

7 ents of autophagy in living cells by optical pulse labeling.

8 d peripheral T cell proliferation by genetic pulse labeling.

9 early folding events has been analyzed by pH-pulse labeling.

10 ction was characterized by hydrogen exchange pulse labeling.

11 ediate was studied by hydrogen exchange (HX) pulse labeling.

12 al DNA synthesis by bromodeoxyuridine (BrdU) pulse-labeling.

13 of 5-bromo-2-deoxyuridine following in vivo pulse-labeling.

14 , RUBISCO, and CBB cycle activities by (13)C pulse-labeling.

15 mide only under chase labeling but not acute pulse labeling, 3) the induction of the levels of sphing

16 under the conditions used for the [(32)P]GTP pulse labeling, (32)P was incorporated into the entire m

17 produce full-length Lhcb as demonstrated by pulse-labeling: a new radioactively labeled band of a si

18 Pulse-labeling alkaline elution showed deficiency of dam

19 The (13)C pulse labeling allowed tracing the C of switchgrass orig

20 Pulse-labeling analyses show that formation of prenyl-pA

21 Pulse-labeling analysis disclosed a time-dependent reduc

22 Pulse-labeling analysis indicated that Nodals and Leftys

23 ll kinetic folding, the present work used HX pulse labeling analyzed by a fragment separation-mass sp

24 After a 30-min [35S]methioneine/cysteine pulse labeling and 120 min of chase, all of the nascent

25 Pulse labeling and chase of mitochondrial translation pr

26 he nonprocessed D1 precursor was observed by pulse labeling and immunodetection in LAHG-grown PS I-le

27 H24L/H119F were studied by hydrogen exchange pulse labeling and interrupted hydrogen/deuterium exchan

28 ompared at high resolution by quench-flow pH-pulse labeling and interrupted hydrogen/deuterium exchan

29 In this study, we employed a combined pulse labeling and neutral-neutral two-dimensional gel-b

30 lts from kinetic deuterium hydrogen exchange pulse labeling and protein engineering studies.

31 i-seq using 5-ethynyl-2'- deoxyuridine (EdU) pulse-labeling and bivariate flow sorting, and while the

32 change using 5-bromo-2'-deoxyuridine (BrdU) pulse-labeling and DAPI (4',6-diamidino-2-phenylindole)

33 ons, Western blot (immunoblot) analysis, and pulse-labeling and immunoprecipitation of both fusion pr

34 We report here the results of pulse-labeling and immunoprecipitation studies of this r

35 Pulse-labeling and metabolic chase analysis suggested th

36 is increased by ppGpp as judged by both RpoS pulse-labeling and promoter-independent effects on lacZ

37 en using substituted pyrimidine analogues in pulse-labeling and suggest that EdU is the preferable nu

38 progression, we followed CHH fibroblasts by pulse-labeling and time-lapse microscopy.

39 Immunoblot, pulse labeling, and ribosome profiling analyses of mutan

40 during superinduction, yet Western blotting, pulse labeling, and the use of bicistronic vectors showe

41 ransfer activity was inhibited at the end of pulse labeling, apoB100 secretion was abolished.

42 producible mass spectrometry-based method, a pulse labeling approach using stable isotope-labeled ami

43 Here, we use a pulse-labeling approach with a monovalent anti-Env Fab p

44 was verified using an in vivo stable isotope pulse-labeling approach, and their exact ribosomal prote

45 onstraining growth conditions in radioactive pulse-labeling approaches.

46 ished proinsulin biosynthesis, observed in a pulse-labeling as short as 5 minutes.

47 siae using a combination of quantitative and pulse labeling-based proteomics approaches, in vitro stu

48 pped-flow fluorescence and hydrogen exchange pulse labeling coupled with mass spectrometry.

49 n T-87 cell lipid assembly were evaluated by pulse labeling cultures with [(14)C]acetate and [(14)C]g

50 Those results, in conjunction with pulse-labeling data, suggest that light elicits a plasto

51 Amino acid pulse labeling directly establishes that much of the ste

52 Previous pulse labeling efforts have always assumed EX2 exchange

53 5 days in labeled medium and also in a 1-day pulse labeling experiment where the washout of label was

54 Here, we revisited the pulse labeling experiment with barnase and detected no s

55 tudies, an amide hydrogen/deuterium exchange pulse-labeling experiment detected a stable submilliseco

56 on of a double-jump experiment followed by a pulse-labeling experiment.

57 e novo sphingolipid pathway as determined by pulse labeling experiments and inhibition studies with m

58 Pulse labeling experiments demonstrated that newly synth

59 nt for NMR measurements after quench-flow pH-pulse labeling experiments gives a greatly increased dat

60 Pulse labeling experiments indicate that int6Delta signi

61 Pulse labeling experiments indicate that LS is synthesiz

62 Hydrogen exchange pulse labeling experiments indicate that, in contrast to

63 Pulse labeling experiments reveal that, in immature cons

64 Pulse labeling experiments revealed that rates of protei

65 tion did not alter PIKK mRNA levels, in vivo pulse labeling experiments showed that Tel2 controls the

66 Furthermore, [(32)P(i)] pulse labeling experiments uncovered alterations in phos

67 In pulse labeling experiments, AAI protected TraR against p

68 labeling by amino acids in cell culture and pulse labeling experiments.

69 Moreover, yeast pulse-labeling experiments argue against there being a s

70 s observed in the previous hydrogen exchange pulse-labeling experiments at pH 6.2 and 10 degrees C.

71 Consistent with this, pulse-labeling experiments demonstrate a significant red

72 [35S]Methionine pulse-labeling experiments demonstrated that GIRK4 assoc

73 uch that the results of our model agree with pulse-labeling experiments from three different nerve ce

74 Strikingly, pulse-labeling experiments indicate that total poly(A)(+

75 Pulse-labeling experiments indicate that ubiquilin facil

76 BrdU pulse-labeling experiments revealed that virtually all s

77 Hydrogen exchange pulse-labeling experiments show that the slow-folding ph

78 Pulse-labeling experiments showed that expression of at

79 Pulse-labeling experiments showed that in vivo CL biosyn

80 Pulse-labeling experiments showed that most major late p

81 Pulse-labeling experiments showed that newly synthesized

82 5-Iododeoxyuridine/5-chlorodeoxyuridine pulse-labeling experiments showed that RAD6 is necessary

83 In pulse-labeling experiments that followed nascent MYOC ov

84 anism, and we perform genetic decoupling and pulse-labeling experiments to demonstrate that Sis1 indu

85 Pulse-labeling experiments using 5-bromo-2-deoxyuridine

86 Results of pulse-labeling experiments with [35S]methionine further

87 the preferable nucleoside analogue for short pulse-labeling experiments, resulting in increased recov

88 Hydrogen exchange pulse-labeling experiments, with NMR detection, were per

89 Hydrogen exchange pulse-labeling followed by mass spectrometry reveals det

90 hin a cell population can be demonstrated by pulse-labeling followed by PCR amplification of immunopr

91 Pulse-labeling HDX-MS studies revealed that ANGPTL3/8 an

92 l phosphates on prothymosin alpha in vivo by pulse-labeling HeLa cells with [32P]orthophosphate and c

93 , and also the recently introduced transient pulse-labeling HX experiments.

94 using circular dichroism, fluorescence, and pulse-labeling hydrogen exchange.

95 Pulse-labeling hydrogen-deuterium exchange experiments m

96 Using pulse-labeling hydrogen-deuterium exchange mass spectrom

97 data set of early folding residues based on pulsed labeling hydrogen deuterium exchange experiments.

98 Uridine pulse labeling in intact CS-B fibroblasts and lymphoblas

99 Using pulse labeling in vivo and synchronized translation in v

100 nt transport waves obtained by radioisotopic pulse labeling in vivo.

101 Pulse-labeling in the presence of cycloheximide indicate

102 no presaturation pulse, (2) a presaturation pulse labeling inferior vena cava (IVC) blood (signal vo

103 Interestingly, shortly after pulse labeling INS cells, a substantial fraction of both

104 Pulse-labeling interaction studies reveal that TMEM126A

105 ombination of 5-bromo-2'-deoxyuridine (BrDU) pulse labeling, intracellular biocytin labeling, and imm

106 e molecules into newly synthesized DNA (i.e. pulse-labeling) is used to monitor cell proliferation or

107 resolution by an advanced hydrogen-exchange pulse-labeling mass-spectrometry method (HX MS).

108 ring folding and used this to provide an NMR pulse labeling method for determining structures of fold

109 Key developments over time include the HX pulse labeling method with nuclear magnetic resonance an

110 Here, we used a pulse-labeling method in Mus musculus models for trackin

111 ic mice overexpressing NF-M by the classical pulse-labeling method using 35S-methionine.

112 ng" method, which is less accurate than the "pulse-labeling" method typically used in mammals.

113 initiation and elongation, as determined by pulse-labeling nucleotide incorporation in replication f

114 rage of approximately 1,100 RS after a 5-min pulse labeling of 3T3 mouse fibroblast cells in early S-

115 In vivo pulse labeling of an mto1 mutant, however, indicate incr

116 Pulse labeling of ATMs with PKH26 assessed the recruitme

117 thesis by TGF-beta and CTGF was confirmed by pulse labeling of cells with [35S]methionine.

118 Bromodeoxyuridine (BrdU) pulse labeling of cortical slices cultured in NMDA antag

119 Pulse labeling of endothelial cells with cholera toxin B

120 Biosynthetic pulse labeling of five human glycoproteins showed that e

121 In this study, we used stable isotope pulse labeling of human pulmonary artery endothelial cel

122 Western blotting experiments and pulse labeling of infected cells with [(35)S]methionine

123 Pulse labeling of mitochondrial protein synthesis produc

124 lable single-nucleus RNA-Seq (sNuc-Seq) with pulse labeling of proliferating cells by 5-ethynyl-2'-de

125 Immediately after UV irradiation and a short pulse labeling of repair patches, intact nuclei were dig

126 n analysis of ATPase transcripts and in vivo pulse labeling of the mitochondrial translation products

127 Pulse labeling of tumor and TDLN T cells with BrdU confi

128 Pulse-labeling of cell wall glucans indicated wall synth

129 he most common nucleoside analogues used for pulse-labeling of DNA in cells are the deoxypyrimidine a

130 Pulse-labeling of DNA with EdU and RNA with BrU and test

131 Pulse-labeling of infected cells revealed that LEF-12 mu

132 Pulse-labeling of lpt1Delta strains showed a 30% reducti

133 Metabolic pulse-labeling of nascent RNA with 4'Thiouridine was use

134 Pulse-labeling of progenitors with bromodeoxyuridine sho

135 tu hybridization analyses to detect mRNA and pulse-labeling of proteins were used to examine several

136 cules, the DNA sequences replicated during a pulse-labeling period.

137 n viral protein accumulation were studied by pulse-labeling proteins in infected protoplasts.

138 Here we use a pulse-block-pulse labeling protocol with fluorescent ligands to labe

139 Using a novel pulse labeling/quantitative mass spectrometry technique,

140 However, short pulse labeling revealed that the initial translation rat

141 Multipoint BrdU pulse-labeling revealed that, compared to cells actively

142 Pulse-labeling RNA studies showed a PARP-dependent incre

143 Pulse labeling showed that the rate of recruitment of ne

144 Second, pulse labeling studies demonstrated increased production

145 Pulse labeling studies indicated that newly replicated m

146 PtdIns dramatically in both steady-state and pulse labeling studies, suggesting that the observed eff

147 Pulse-labeling studies and immunoblot analyses showed th

148 We have used pulse-labeling studies and the expression of the ARG8(m)

149 m unloading, making it a suitable tracer for pulse-labeling studies of phloem transport.

150 Pulse-labeling studies reveal that extracellular secreti

151 Pulse-labeling studies show that when K(IR)6.2 is expres

152 Results from our pulse-labeling studies showed that Cdc37 protects nascen

153 BrdU, although cell cycle analyses and BrdU pulse-labeling studies suggested that most of this proli

154 d stability of the molecule, as indicated by pulse-labeling studies, demonstrating a prolonged half-l

155 For the G8 mutant, from these assays and pulse-labeling studies, we determined the ratio of synth

156 By performing BrdU pulse-labeling studies, we found that MBC formation prec

157 ntracellular betaPPs and Abeta shortly after pulse labeling suggests that Abeta is produced in the se

158 blood (signal void), and (3) a presaturation pulse labeling superior vena cava (SVC) blood.

159 use footpads using a newly developed in situ pulse labeling technique.

160 Here we used steady-state and pulse-labeling techniques to follow Notch receptors in s

161 the IL-6(-/-) mice show more hepatocyte BrdU pulse labeling than the IL-6(+/+) controls at 24 hours,

162 Within 10 min of pulse labeling, the mutant protein undergoes a molecular

163 tivities with gp160 at different times after pulse-labeling, the MAbs were sorted into groups that ex

164 We use live imaging and pulse labeling to quantitatively determine the fates of

165 Using optical pulse labeling to visualize the turnover of endogenously

166 Thus, we were able to use FSPM pulse-labeling to localize PBP4 activity in live cells,

167 Hydrogen exchange pulse labeling was used to establish the structure of th

168 baculoviruses (BV) were plaque purified, and pulse-labeling was used to verify the synthesis and secr

169 hetic rate, measured using [(35)S]methionine pulse labeling, was decreased by 75% in the diabetic adi

170 Using SNAP-based fluorescent pulse labeling, we now demonstrate that cell cycle-restr

171 Here, using 13CO2 pulse labeling, we show that natural densities of the nu

172 ng intermediates were probed by H/D exchange pulsed labeling, which showed the coexistence of noncomp

173 Pulse labeling with 5-EU revealed nascent and unstable R

174 Pulse labeling with [(35)S]methionine and biotinylation

175 The 41-kDa species, emerging within 5 min of pulse labeling with [(35)S]methionine, is converted into

176 y incorporated into camalexin during a 1.5-h pulse labeling with [14C]anthranilate also increased wit

177 incorporation into camalexin during a 1.5-h pulse labeling with [14C]indole was similar to that with

178 TP photoaffinity inhibitor BMS-192951 before pulse labeling with [35S]methionine/cysteine led to an 8

179 Following pulse labeling with [3H]arachidonic acid ([3H]AA), its i

180 Single pulse labeling with [3H]thymidine at 36 h labeled Thy 1-

181 Pulse labeling with [3H]thymidine confirmed in vitro neu

182 Single as well as multiple pulse labeling with [3H]thymidine confirmed that the ent

183 ease H was studied by hydrogen exchange (HX) pulse labeling with analysis by an advanced fragment sep

184 n 17 HIV-infected patients by 30 min in vivo pulse labeling with bromodeoxyuridine (BrdU).

185 x HIV-1-infected patients studied by in vivo pulse labeling with bromodeoxyuridine.

186 Recent work using HX pulse labeling with MS analysis finds that a number of p

187 ucted by genome-wide molecular combing after pulse labeling with two thymidine analogues.

188 Pulse-labeling with (15)N-labeled medium time-stamps the

189 in guanidine hydrochloride (GdHCl) or urea, pulse-labeling with 2H2O and analyzing the intact protei

190 lutions of TIM unfolded in GdHCl or urea and pulse-labeling with 2H2O at different times.

191 ing after 24 hours was preceded by 2 hour of pulse-labeling with 5-bromodeoxyuridine.

192 smission of herpes simplex virus (HSV) using pulse-labeling with ethynyl nucleotides and cycloadditio

193 ra, transducin activation, Western blotting, pulse-labeling with immunoprecipitation, and immunocytoc