ライフサイエンスコーパス: purify

1 in Escherichia coli BL21(DE3), refolded, and purified.

2 PKG stimulated purified 26S proteasomes by phosphorylating a different

3 ibosomal subunits in vivo and can dissociate purified 70S ribosomes in vitro, independent of GTP hydr

4 concentrations in crude (488.93mug/g DM) and purified (8394.99mug/g DM) extracts.

5 To test our prediction, we purified a candidate beta-lactone synthetase from Nocard

6 To address this question, we purified a five-member Pseudomonas aeruginosa division c

7 ~5% conversion of precursor to O-glycans and purified a tagged O-glycan library of over 100 O-glycan

8 Here, titrations with the purified ACCase biotin attachment domain-containing (BAD

9 n network mechanics is well-characterized in purified actin networks, its mechanical role in the cyto

10 Here, we expressed and purified active SARS-CoV-2 RdRp composed of the nonstruc

11 Using purified adult retinal ganglion cells (RGCs) in culture,

12 We subsequently purified an activity from HeLa cell extracts and identif

13 Here, we purified an HAO from the thermophilic verrucomicrobial m

14 Here, we purify an intact T4CC from the Legionella membrane.

15 noglobulin G from 18 acute iTTP patients was purified and added to closed ADAMTS13 in healthy donor p

16 ystem, human GLS2 protein was overexpressed, purified and analyzed for posttranslational modification

17 veral hours explains why SznF was previously purified and crystallographically characterized without

18 es and drawbacks of the two major strategies-purified and extract-based systems-and how they may be u

19 Total RNA collected from cultured islets was purified and global miRNA profiling was performed with 3

20 inking reaction so that they can be affinity purified and identified by mass spectrometry (eLife 2015

21 her with Orco, were recombinantly expressed, purified and integrated into liposomes.

22 finity tags introduced at the Tet2 locus, we purified and sequenced TET2-crosslinked RNAs from mouse

23 Here we purified and spectroscopically characterized light-harve

24 Three of these peptides were purified and their structures elucidated by one and two-

25 Toward that end here we purify and characterize a suite of 33 Arabidopsis Rubisc

26 Here we present validated methods to purify and culture primary HLA-G+ EVT from the placental

27 f HbF activation by developing techniques to purify and profile differentiation stage-matched late er

28 olution (i.e., selective sweeps), widespread purifying and background selection, and, more recently,

29 We then synthesized, purified, and biochemically tested the carboxylation rat

30 ress this limitation, here we overexpressed, purified, and characterized G. lovleyi NrfA.

31 The recombinant FrtR protein was purified, and conserved DNA binding motifs were determin

32 Nisin P was purified, and its predicted structure was confirmed by n

33 here optimized protocols used to synthesize, purify, and characterize a panel of six Yariv reagents a

34 strate that autoSTOMP can efficiently label, purify, and identify proteins belonging to 1-2 mum struc

35 tion, by spiking in known amounts of HCPs to purified antibody drug substance with low levels of HCPs

36 hemical and thermal stability studies of the purified ARD by the means of circular dichroism and tryp

37 Recombinant NFU4 and NFU5 were purified as apo-proteins after expression in Escherichia

38 Polyamines stimulate the activity of purified ATP13A2, whereas ATP13A2 mutants that are impli

39 Furthermore, purified axoplasm exhibits adenosine-to-inosine activity

40 Purified B cells from such patients generated more IL-35

41 We hypothesized that purified bacterial collagenase would initiate nociceptiv

42 We then transfer the purified bacterial DNA to our microfluidic nanoarray to

43 observed between the inhibition efficacy of purified beta-lactamases and the potentiation of beta-la

44 Assays were established to generate and purify both naturally occurring and in silico-designed D

45 By reconstituting these processes with purified budding yeast proteins, we show that ubiquityla

46 produced initially with a histidine tag and purified by a simple two-step procedure.

47 rologously expressed in Escherichia coli and purified by affinity chromatography with a yield of 100

48 Released O-glycans were purified by an optimized protocol to eliminate interfere

49 pressing cell-specific fluorescent reporters purified by fluorescence activated cell sorting (FACS).

50 tissue, greater than 10(4) eosinophils were purified by fluorescence-activated cell sorting (FACS) p

51 ELMOD2 is a ~32 kDa protein first purified by its GTPase-activating protein (GAP) activity

52 (C(16:0)) and stearic (C(18:0)) fatty acids purified by preparative gas chromatography(5-8).

53 crosslinked RNA binding proteins (RBPs) are purified by solid-phase reversible immobilization (SPRI)

54 Bipods purified by this method have purity suitable for cell-ba

55 ediment-containing fraction could be however purified by ultracentrifugation resulting in the mitigat

56 Radiotracers were purified by using size-exclusion methods and evaluated b

57 Activated purified CaMKIIalpha also directly binds Shank3 between

58 Purified CD8(+) T cells from controls respond to opioids

59 ized for temperature and reaction time using purified cDNA and viral RNA as template.

60 out the need for DNA methylation profiles of purified cell types.

61 mes, also acted as trigger wave sources, but purified centrosomes in the absence of nuclei did not.

62 , a STING-mediated method to concentrate and purify cGAMP from any type of biological sample.

63 nd doxycycline on the antibody response to a purified chick embryo cell vaccine, given on a postexpos

64 er plants to produce steam and transport the purified CO(2) via trucks to the nearest opportunity for

65 ) uptake into proteoliposomes containing the purified complex.

66 bacterial cell division machinery using its purified components FtsZ, FtsA and truncated transmembra

67 A palmitoylation assay with purified components revealed that Galphas and Galphai ar

68 rization of a subset of Cas9 orthologs using purified components reveals additional biochemical diver

69 nstituting translation in vitro using highly purified components, we show that this translation block

70 cesses of HIV-1 assembly and maturation from purified components.IMPORTANCE Assembly and maturation a

71 culation with radiation-attenuated, aseptic, purified, cryopreserved Plasmodium falciparum (Pf) sporo

72 fSPZ Vaccine (radiation attenuated, aseptic, purified, cryopreserved Plasmodium falciparum [Pf] sporo

73 In addition, purified CST complex binds to 5' DNA overhangs and direc

74 Using the CTC-iChip to purify CTCs from PDAC patients for RNA-seq characterizat

75 To purify cutin we tested the ionic liquids cholinium hexan

76 These studies were conducted using purified DGKepsilon and detergent-free phospholipid aggr

77 site-specific endonuclease strand-breaks in purified DNA from Eschericia coli, phosphorothioate epig

78 U3 activates purified DNA-PK and triggers phosphorylation of DNA-PKcs

79 We reconstituted the PT reaction using purified DndCDE from Salmonella enterica and IscS from E

80 ermeable probe showed good reactivity toward purified DUBs, including USP2, UCHL1, and UCHL3, upon ph

81 Mitochondria were purified either with discontinuous Percoll gradient or w

82 Activity assays using the purified enzyme confirmed that angiotensin peptides are

83 e unrelated cell types (mosaic data) or from purified enzyme data.

84 The purified enzyme specifically recognises ss-dsDNA junctio

85 Experiments with purified enzymes indicated that viperin inhibits the thi

86 ults also suggest that, although some of the purified enzymes involved in alanine biosynthesis have b

87 generate donor and acceptor substrates using purified enzymes of the ALG pathway to recapitulate the

88 diseases by direct optimization using single purified enzymes.

89 Using purified erythroid progenitors in vitro, we show that IL

90 e results from a biochemical analysis of the purified Escherichia coli YecSC-FliY cysteine/cystine im

91 s has been complicated by limited amounts of purified EVs, low-abundance PTM proteins, and interferen

92 ggshell matrix proteins in the uterus and in purified EVs.

93 of HCC by performing digital scoring on the purified EVs.

94 o ongoing analytical efforts to separate and purify exosomes and exosome subpopulations.

95 The purified extracts of Cerise and Black Prince tomatoes ha

96 Ethidium Bromide uptake in purified F4/80+/CD11b+ mdx macrophages revealed function

97 ter-proximal pausing in vitro, entirely with purified factors (no extracts).

98 e investigated the biochemical properties of purified FCP complexes, but limited knowledge is availab

99 CD49f can be used to purify fetal astrocytes and human induced pluripotent st

100 n, we performed ATAC-seq from bulk tissue or purified fibroblasts of uninjured and regenerating cauda

101 the limited availability of glycans in their purified form, the low affinities of GBP-glycan interact

102 dered by its instability and insolubility in purified form.

103 Experiments performed with purified Fpn provided direct evidence that the protease

104 We validated that A250, a highly purified fraction of fermented wheat germ extract (FWGE)

105 erical piliferous virus 1 (TSPV1), which was purified from a Thermoproteales host isolated from a hot

106 IgE-reactive proteins were purified from almond kernels.

107 itive in detecting Ab-54149 than an antibody purified from anti- Ab-54149 EPS serum.

108 chnological potential of a cysteine protease purified from Calotropis procera (CpCP3).

109 Ctf19c members associate hierarchically when purified from cell extract [1], an observation that is m

110 Tag ligand, Ago2-miRNA-mRNA complexes can be purified from cells and tissues expressing the endogenou

111 MDMX purified from cells contains near-stoichiometric amounts

112 iled functional analyses of insulin granules purified from cells subjected to model treatments that m

113 Bovine colostral antibodies, purified from cow's milk produced immediately after calv

114 Fontenot et al. now show that Fur purified from Escherichia coli binds an all-Cys-coordina

115 Importantly, the transfer of EVs purified from HCMV-infected cells enhanced virus spread.

116 e involved the use of urea-denatured protein purified from inclusion bodies.

117 Fluorescent staining of virus-like particles purified from infant meconium or early stool samples sho

118 in peroxidase (LiP), an extracellular enzyme purified from Phanerochaete chrysosporium NK-1 isolated

119 ere, ~39 kDa class IV chitinase (HrCHI4) was purified from seabuckthorn seeds using chitin-affinity c

120 Other plasma MPs were purified from SS patients before and 2 years after the o

121 studied; in addition, dendritic cells (DCs) purified from the draining lymph nodes of tape-stripped

122 res were seeded with ex-vivo amyloid fibrils purified from the explanted heart of this patient.

123 ehydrogenase (suggested EC 1.8.2.7) that was purified from the haloalkaliphilic sulfur-oxidizing bact

124 We demonstrate that exosomes purified from the saliva of HIV-positive patients and se

125 human lubricin (rhPRG4) and native lubricin purified from the SF of patients were incubated with exo

126 The native receptor was purified from Torpedo electric tissue and functionally r

127 Addition of agonist and the purified G(s) protein surrogate mini-G(s) (mG(s)) yielde

128 In addition, we purified genomic DNA from paraffin-embedded tissue and p

129 n this study, we constructed a compendium of purified ginsenoside compounds from Panax ginseng C.A. M

130 of ~ 1,500 transmembrane proteins using the purified GluN1-NTD protein as a bait, we identify Protoc

131 slational modifications for stabilization of purified GPCR in detergent micelles.

132 The limited availability of purified GSLs represents a significant challenge to the

133 The purified HCC EVs are subjected to reverse-transcription

134 rate different combinations of extracted and purified hemicelluloses (xylans and glucomannans) from s

135 compared to the desuccinylation reaction of purified His-SIRT5.

136 Here we purify holo-CCP2 directly from cyanobacteria and establi

137 The purified HoxEFU subcomplex catalyzed electron transfer r

138 esent a structural model of the endogenously purified human canonical BAF complex bound to the nucleo

139 We purified human gammaTuRC and measured its nucleation pro

140 red to form cccDNA by both cell extracts and purified human proteins.

141 In contrast, the addition of purified hyaluronan suppresses excitatory synapse format

142 Purified HypX proved to be metal-free, which makes it a

143 93F mammalian cell cultures, solubilized and purified in Facade detergent.

144 bit warfarin sensitivity, whereas human VKOR purified in LMNG is stable only with pre-bound warfarin.

145 The protein could be extracted and purified in surfactant micelles and was monodisperse and

146 It co-purifies in vivo with DNMT3L and DNMT3A, components of t

147 show that a single-dose immunization of ZIKV purified inactivated vaccine (ZPIV)(4-7) in a dengue vir

148 efficacy of an aluminum hydroxide adjuvanted purified inactivated Zika vaccine (PIZV) against challen

149 ) amount in the vaccine without the need for purified, inactivated, and calibrated virus reference an

150 We purified ISCs and intestinal progenitors using flow cyto

151 Similarly, purified kallikrein activated FIX in buffer and initiate

152 The effect of purified KCNQ1 antibodies on human cardiomyocytes derive

153 CaRe purifies lectins by utilizing their ability to form spon

154 CaRe is a time-saving procedure that can purify lectins even from a few milliliters of crude prot

155 mia control, we evaluated gene expression of purified leukocyte subsets from IC versus NUC-treated pa

156 The purified Lon protease degraded free H-NS but not DNA-bou

157 Addition of purified LOXCAT to the medium of cultured human cells wi

158 ne and ATP, protected against ALI induced by purified LPS.

159 this process was established by transferring purified M2 macrophages from PAM3 treated control donors

160 of mice with COBRA P1 and the corresponding purified mAbs were characterized for Ag specificity and

161 results, which are from assays performed on purified melanoma cell lines, suggest that the TERT prom

162 More importantly, by purifying microglia/macrophage derived exosomes in the C

163 The purified mitochondria were suitable for enzyme activity

164 olitica-infected bone marrow-derived DCs and purified MLN DCs, which may explain the mechanism underl

165 proach designed to optimize HCP detection in purified monoclonal antibody samples with LC-MS(2).

166 helicase activity, dissociation of UPF1 from purified mRNPs, and transcriptome-wide UPF1 RNA binding,

167 This raises the question of whether purified muscle myosins retain procoagulant phospholipid

168 This work provides a framework to generate purified N(0) and incorporate it with RNA into NCLPs in

169 Furthermore, COM1 is subject to purifying natural selection, thereby contributing to spe

170 Highly purified neutrophils from healthy donors were primed in

171 ning CCR4 family of deadenylases, but highly purified NOC possesses little or no ribonuclease activit

172 The purified nsLTP1 was treated with 4-vinyl pyridine, and t

173 ediment containing fractions compared to the purified oil.

174 hen CaM is bound to full-length RyR1, either purified or in SR membranes, strikingly different result

175 ed in cellular membranes without the need to purify or label the investigated membrane protein.

176 required in the context of the full-length, purified Par-6-aPKC complex.

177 illus subtilis species, using live cells and purified peptidoglycan.

178 rn blot, and RT-PCR were performed in highly purified peripheral blood basophils and eosinophils of a

179 tants together with biochemical studies with purified phospholipases, we investigate the effect of th

180 -C1), increases the lipid kinase activity of purified PI3KC3-C1, and is required for endosomal genera

181 Proteome analysis of purified podocyte fractions from FSGS mouse models showe

182 y proteome analysis to very small samples of purified podocyte fractions.

183 irely differently in human cells than in the purified Pol establishes a new paradigm for DNA polymera

184 Under identical conditions, purified Pol I and Pol III, but not Pol II, could transc

185 heta differs from other Pols in that whereas purified Poltheta misincorporates an A opposite 1,N (6)-

186 ture CSA-Flow allows for isolation of highly-purified populations of both low-frequency (IL-10(+); GM

187 Purified prodigiosin and ShlA from Escherichia coli furt

188 Purified prodigiosin was obtained from stationary phase

189 DNA sequencing of purified products yielded Phred quality scores values of

190 in E. coli and spectroscopic analysis of the purified products.

191 are used in many research schemes including purifying products from reaction mixtures, fractionating

192 Vts1 condensates formed from purified protein can transform naive cells to acquire [S

193 ons by two-hybrid techniques and of affinity-purified protein complexes.

194 onstituting the NOCAP synthase in vitro from purified protein components followed by heterologous exp

195 tality while inducing more BCG reactions and purified protein derivative (PPD) responses (secondary o

196 Purified protein from Escherichia coli showed robust sta

197 N- and C-termini has been limited to single purified protein species, while the proteome-wide identi

198 pathway activity, and controlling binding to purified protein targets in vitro.

199 Here, using purified proteins and several biochemical assays and sur

200 In this study, using purified proteins and site-directed mutagenesis, along w

201 d the development of M1 assembly assays with purified proteins are crucial advances for antiviral tar

202 a parS-dependent ParB spreading event using purified proteins from Caulobacter crescentus and show t

203 The higher yields of purified proteins obtained with constructs lacking the l

204 on, localization and transport function, and purified proteins retained drug stimulation of ATP hydro

205 ing site for Rap1A, we first confirmed using purified proteins that the RA2 domain is indeed essentia

206 We used yeast proteome microarrays (~5800 purified proteins) to conduct a high-throughput and syst

207 Here we use purified proteins, mouse primary neurons and patient-der

208 o directly screen structural features of pre-purified proteins, protein complexes or clarified cell l

209 Here, using recombinantly expressed and purified proteins, static light-scattering analysis, and

210 Using purified proteins, we confirm that RPA inhibits A3A by b

211 Using purified proteins, we report a minimal cell-free system

212 Using purified proteins, we show that although DEP1 phosphoryl

213 interaction as supported in experiments with purified proteins.

214 hrough high resolution structural studies of purified proteins.

215 formation in human cells and in vitro using purified proteins.

216 formation and potential heme transfer using purified proteins.

217 Then, a study involving purified PRPs and polyphenols from apple juice was condu

218 We established that the largest purified PSI-LHCI supercomplex contains 10 LHCIs (~240 c

219 A known amount of purified quebracho tannin was used to test the validatio

220 rRNA promoters were activated by purified R. sphaeroides CarD, a transcription factor fou

221 ons and deletions in vitro by reconstituting purified RD3 variants with GCAP1-activated human RetGC1.

222 We have studied the role of purified recombinant alpha-hemolysin in inducing inflamm

223 directly incubating the macrophages with the purified recombinant EsxA or EsxB protein or the EsxBA h

224 Here, using purified recombinant human proteins, we uncover a role f

225 chia coli Functional characterization of the purified recombinant proteins revealed that NnOMT1 is a

226 Biochemical studies on purified rhodopsin from mice indicated that multiple spe

227 e of detergent, the C protein is retained on purified ribonucleocapsids less efficiently than the P p

228 Using an in vitro transcription system with purified RNA polymerase (RNAP) to investigate rRNA synth

229 for in vitro de novo RNA synthesis using the purified RSV polymerase as 8 nucleotides (nt), shorter t

230 on up to 66.4% and 24% yield of isolated and purified rutinoside.

231 s when CaM is bound to RyR1 in SR membranes, purified RyR1, or a peptide corresponding to the CaM-bin

232 and conformations of soluble, overexpressed, purified S proteins have been studied in detail using cr

233 erase chain reaction (RT-qPCR) to detect the purified SARS-CoV-2 RNA.

234 The purified scFvs showed specific activity towards alpha-sy

235 population size changes and the strength of purifying selection affect patterns of variation at "neu

236 ma as the deletion driver and suggest little purifying selection against mtDNA deletions by mitophagy

237 ear incompatibilities, hybrid breakdown, and purifying selection as mechanisms that can support speci

238 There is evidence of purifying selection but little evidence of diversifying

239 Similar alterations in the strength of purifying selection during cancer development could help

240 were created by mutational bias coupled with purifying selection on the protein code.

241 growth than was inferred when the effects of purifying selection were neglected.

242 plasmy in T cells, a finding consistent with purifying selection within this lineage.

243 al in multimers but deleterious in monomers; purifying selection would then prevent reversion to the

244 e demographic history of the population) and purifying selection, is lacking.

245 ven species of Diadema, bindin evolves under purifying selection, more slowly than in any other sea u

246 nsights into mechanisms of TE regulation and purifying selection, which reveal the remarkable foresig

247 All PCGs showed signs of purifying selection, with K(A)/K(S) <<1 across the whole

248 hancers show evidence of unusually stringent purifying selection.

249 ppose TE spread: regulated transposition and purifying selection.

250 nalysis show that ORF-Y is subject to strong purifying selection.

251 RNA-seq on non-purified sensory neuronal preparations, such as whole do

252 Exposing macrophages to purified SL-1 enhanced the trafficking of phagocytic car

253 We purified SMALP-lipid-protein complexes by chromatography

254 Purified Smc5/6 exhibits DNA-dependent ATP hydrolysis an

255 We interrogated purified sorted tumor fractions from these samples with

256 inferred from high resolution structures of purified stable intermediates, low resolution images of

257 ice were either euthanized (baseline) or fed purified standard diet with or without F1394 for 4 more

258 nsive and labor-intensive and require highly purified starting material.

259 origami structures, and stepwise assembly of purified structures with coiled-coil conjugates.

260 Prolonged culture also revealed that purified subpopulations of MDSCs exhibit remarkable plas

261 gated this with specific comparison with the purified synthetic TLR2 agonists, Pam(3)CSK(4) and FSL-1

262 we show interacts with IER5 in cells and in purified systems.

263 cells isolated from the immunized camel and purified the antibody from Escherichia coli after refold

264 Here we purified the budding yeast Smc5/6 holocomplex and charac

265 sed them heterologously in Escherichia coli, purified the corresponding recombinant enzymes, and perf

266 We first purify the bacterial DNA into a PCR solution using our D

267 sing a diverse combination of techniques, we purified these serpins from several species of nonmodel

268 m (NKX2-1(GFP);TP63(tdTomato)), we track and purify these cells as they first emerge as developmental

269 ransgenic line with fluorescent oenocytes to purify these cells for the first time.

270 as extracting solvent and the extracts were purified through primary secondary amine and graphitized

271 Using genome-wide RNA-Seq analysis of purified Tregs sorted from multiple human cancer types,

272 lar to those of the hexagonal nets formed by purified TRIM5alpha in vitro.

273 ctures of the binding domains alone and with purified trisaccharides.

274 Purified UNC-45B mutants showed changes in folding and s

275 we report that human recombinant beta(2)GPI purified under native conditions is oxidized.

276 The extracted ergosterol was purified using a novel methodology and the extraction so

277 Nucleic acid is purified using a pipet tip with an embedded matrix that

278 Fennel nsLTP1 was purified using the combination of gel filtration and rev

279 Studies with purified UVR8 and seedlings not previously exposed to UV

280 a direct interaction between vancomycin and purified VanS(SC), and traced these interactions to the

281 on protein modeling and in vitro analysis of purified variants.

282 ethod for the quantification of a variety of purified vesicle samples.

283 Scandium radionuclides were purified via ion-exchange chromatography using branched

284 uman adenovirus type 5 (AdV5) offered highly purified virus for the subsequent fluorescent labeling p

285 We purified virus-like particles (VLPs) from ddm1 and ddm1r

286 AP proteins and verified lattice dynamics in purified VLPs incorporating 10% Gag-SNAP, 10% Gag-Halo,

287 t the cryo-electron microscopy structures of purified VZV A-capsid and C-capsid, as well as of the DN

288 ormations in the oceans played a key role in purifying water from toxic impurities and, thus, played

289 cleoli in an rRNA-dependent manner and is co-purified with the small subunit processome.

290 Here, we show that TDP2 co-purifies with K63 and K27 poly-Ubiquitinated cellular pr

291 alysis identified a total of 181 proteins co-purifying with a Venus multifunctional (VM)-tagged CK1de

292 rodimer, cumulin is difficult to produce and purify without contaminating GDF9 and BMP15 homodimers.

293 and FZDs has been hampered by the paucity of purified WNT proteins and by their apparent non-selectiv

294 o directly visualize nucleation of a MT from purified Xenopus laevis gamma-TuRC.

295 Using purified Xenopus laevis proteins we biochemically recons

296 tro initiation reaction which is set up with purified yeast components and designed to stall with eIF

297 nthesis in a reconstituted system containing purified yeast F(1)F(0) ATP synthase, although, thermody

298 RBD protein fragment that is expressed at a purified yield of 214 mg/l in unoptimized, mammalian cel

299 Methods: CR3022 was labeled with (131)I and purified, yielding (131)I-CR3022.

300 with zDHHC20, and MBLAC2 is a substrate for purified zDHHC20 in vitro.