ライフサイエンスコーパス: pyrosequence

1 pigenetic measurements, DNA was isolated and pyrosequenced.

2 egion of the 16S rRNA gene amplified and 454-pyrosequenced.

3 molecular phylogenetic analysis of 16S rDNA pyrosequences.

4 sine methylation was measured in blood using pyrosequencing.

5 (96/126) with follow-up target-specific PCR/pyrosequencing.

6 first examination were analyzed by ultradeep pyrosequencing.

7 All controls screened negative by PCR/pyrosequencing.

8 analyzed by 16S ribosomal RNA gene amplicon pyrosequencing.

9 re collected and profiled with 16S rRNA gene pyrosequencing.

10 fic DNA methylation analysis in plants using pyrosequencing.

11 ent in sorted cells from spleen and blood by pyrosequencing.

12 olution using high-throughput locus-specific pyrosequencing.

13 ection or a limitation of the sensitivity of pyrosequencing.

14 Findings were validated using bisulfite pyrosequencing.

15 The microbiota was monitored using 454 pyrosequencing.

16 e expression, and top CpGs were validated by pyrosequencing.

17 The methylation status was confirmed by pyrosequencing.

18 ks and 6 months by 16S-based GS-FLX-titanium-pyrosequencing.

19 as performed on cellular RNA using Roche/454 pyrosequencing.

20 ory protocol) and by bacterial 16S rRNA gene pyrosequencing.

21 ic, with validation of findings by bisulfite pyrosequencing.

22 ma RNA and cell DNA specimens by OLA and 454 pyrosequencing.

23 A, and one at 69% by OLA and not detected by pyrosequencing.

24 es in the leptin promoter was measured using pyrosequencing.

25 ed annually between 5 and 14 years of age by pyrosequencing.

26 (n = 73) and at age of 4.5 years (n = 61) by pyrosequencing.

27 Clostridiaceae) inferred from 16S rRNA gene pyrosequencing.

28 nd 24 months were characterized by 16S-based pyrosequencing.

29 ta, across this landscape using qPCR and 454 pyrosequencing.

30 pG sites within the SFRP1 promoter region by pyrosequencing.

31 ota composition (dysbiosis) was evaluated by Pyrosequencing.

32 o those that were previously described using pyrosequencing.

33 ate landscape using 16S rRNA gene tagged 454 pyrosequencing.

34 A quantitative polymerase chain reaction and pyrosequencing.

35 mpared with standard single-region Roche 454 Pyrosequencing.

36 g enzymes and transporters were evaluated by pyrosequencing.

37 eir promoter regions was analyzed by PCR and pyrosequencing.

38 mor tissues and 9 matching normal tissues by pyrosequencing.

39 was compared to quantitative PCR (qPCR) and pyrosequencing.

40 rongosa National Park, Mozambique) using 454 pyrosequencing.

41 tissues of the offspring were determined by pyrosequencing.

42 d and analyzed by 16S ribosomal RNA targeted pyrosequencing.

43 Microbial taxa were identified by 16S pyrosequencing.

44 y and environmentally relevant species using pyrosequencing.

45 arate validation cohort of 30 MF patients by pyrosequencing.

46 s DHAs on DNA methylation reversal using DNA pyrosequencing.

47 C4 in response to exposure were validated by pyrosequencing.

48 vered Antarctic lakes by 16S rRNA gene-based pyrosequencing.

49 ity was tracked using 16S rRNA gene amplicon pyrosequencing.

50 munity data from two distinct studies by 454 pyrosequencing.

51 a viridis and Actinia equina using 16 S rDNA pyrosequencing.

52 n additional tumor samples using RT-qPCR and pyrosequencing.

53 r working with the raw flowgrams obtained by pyrosequencing.

54 16S rRNA gene polymerase chain reaction and pyrosequencing.

55 Candidate genes were validated by bisulfite pyrosequencing (30 AGA, 21 SGA) and also analyzed in cor

56 For 42 real pyrosequencing ('454') datasets, assembly increased the

57 ); among 60 (61%) A (H3N2) viruses tested by pyrosequencing, 53 (88%) belonged to the drifted 3C.2a g

58 ssay results with phenotypic DST (97.0%) and pyrosequencing (97.8%) results from the same clinical sa

59 Third, pyrosequencing allowed the detection of OTUs that were e

60 Our 16S rRNA gene pyrosequencing analyses revealed that the soil type dete

61 Based on pyrosequencing analyses, beta-Proteobacteria dominated i

62 Pyrosequencing analysis and qPCR revealed a dramatic tra

63 AMF diversity was determined using 18S rDNA pyrosequencing analysis of 360 soil samples taken at six

64 Pyrosequencing analysis of CpG rich regions, and CpG din

65 16S rDNA metagenomic pyrosequencing analysis of the microbial communities ass

66 Pyrosequencing analysis revealed that Geobacter species

67 Methylation-sensitive pyrosequencing analysis showed that CpG sites at the Thy

68 Pyrosequencing analysis was conducted to determine the s

69 Using highly quantitative pyrosequencing analysis, we found extensive and highly v

70 dividuals for miRNA expression profiling and pyrosequencing analysis.

71 ribed algorithms to de-noise functional gene pyrosequences and performs ecological analysis on de-noi

72 mucosal microbiota assessed by 16S rRNA gene pyrosequencing and (1)H NMR spectroscopy.

73 The application of pyrosequencing and allele-specific PCR techniques establ

74 etagenomic shotgun sequencing, 16S rRNA gene pyrosequencing and cloning/sequencing hgcAB gene product

75 ched genome-wide significance, replicated by pyrosequencing and correlated with expression difference

76 ormed on stool samples via 16S ribosomal RNA pyrosequencing and correlations between disease severity

77 Concordance between PCR/pyrosequencing and culture was 96.9% (1,085/1,120) for U

78 (n = 15) were analyzed by 16S ribosomal RNA pyrosequencing and culture-based methods.

79 Bisulphite pyrosequencing and demethylation treatment showed that N

80 umole photons s(-1) m(-2) ) and combined 454-pyrosequencing and enzymatic activity assays to evaluate

81 ological settings by 16S rRNA gene-based 454 pyrosequencing and explored their relationships to filtr

82 Pyrosequencing and fluorescence in situ hybridization an

83 particle purification, genome amplification, pyrosequencing and gene/genome reconstruction and annota

84 me were investigated using 16S rRNA amplicon pyrosequencing and high-throughput quantitative PCR.

85 16S ribosomal RNA gene amplicon pyrosequencing and HPV DNA testing were conducted annual

86 firmed sepsis samples with Universal 16S PCR/pyrosequencing and in 76.4% (96/126) with follow-up targ

87 ycobacterium tuberculosis isolates combining pyrosequencing and IS6110 polymorphism.

88 Here, we used 16S rRNA gene pyrosequencing and metagenomic sequencing to examine ora

89 trations of approximately 2%, one at 8.8% by pyrosequencing and not detected by OLA, and one at 69% b

90 levels of resistant variants not detected by pyrosequencing and possibly below the threshold for phen

91 Pyrosequencing and qPCR revealed that laundry water micr

92 hberg) and verified hits using bisulfite PCR pyrosequencing and qPCR.

93 ces samples were analyzed by high-throughput pyrosequencing and quantitative real-time polymerase cha

94 thylation and mRNA levels by using bisulfite pyrosequencing and quantitative RT-PCR in monocytes in v

95 sing over 7 million cDNA sequences from both pyrosequencing and Sanger sequencing, we found that a mu

96 According to 16S rRNA pyrosequencing and shotgun metagenome sequencing analyse

97 We applied 454-pyrosequencing and single-cell RT-PCR of bulk and sorted

98 ymorphisms were typed using a combination of pyrosequencing and TaqMan genotyping assays.

99 flocks was performed using Illumina and 454 pyrosequencing and the sequenced genomes compared to the

100 e PCR), DNA methylation status (by bisulfite pyrosequencing), and GAL peptide by RIA of the GAL syste

101 DMP was successfully validated by bisulfite-pyrosequencing, and identified DMPs were tested in postm

102 n primers, multiple DNA barcode markers, 454-pyrosequencing, and Illumina MiSeq sequencing to the sam

103 r 16S rRNA gene techniques (clone libraries, pyrosequencing, and real-time PCR), we show that polymet

104 pecific polymerase chain reaction, bisulfite pyrosequencing, and restriction enzyme-polymerase chain

105 tractive hybridisation (SSH) followed by 454 pyrosequencing, and RT-qPCR methods.

106 uantitative polymerase chain reaction (PCR), pyrosequencing, and standard culture techniques.

107 ogical studies, we developed and validated a pyrosequencing approach that enables identification of s

108 cts were sequenced using both Sanger and 454 pyrosequencing approaches.

109 ns identified by Universal 16S rRNA gene PCR/pyrosequencing as containing staphylococci, streptococci

110 e promoter of ADCYAP1R1 (cg11218385) using a pyrosequencing assay in DNA from white blood cells.

111 A pyrosequencing assay was established that reproduced the

112 A novel pyrosequencing assay was used to determine genetic group

113 ured by bisulphite-polymerase chain reaction pyrosequencing assay.

114 ions were verified by MassARRAY Epityper and pyrosequencing assays and could be further replicated in

115 R amplification on bisulfite-treated DNA and pyrosequencing assays as well as the quantification of t

116 A methylation using bisulfite conversion and pyrosequencing assays on DNA from buccal cells provided

117 distinguish these variants reliably and thus pyrosequencing assays were developed.

118 ee sources are suitable for standard PCR and pyrosequencing assays.

119 cortex and performed quantitative bisulfite pyrosequencing at nine loci.

120 The virus populations were sequenced by 454 pyrosequencing at the end of passaging, and the results

121 s readily adopted by another laboratory with pyrosequencing capabilities.

122 riteria for pyrosequence processing or among pyrosequencing, cloning and microscopy, while taxon iden

123 ely 7.5 h when including target-specific PCR/pyrosequencing compared to 27.9 +/- 13.6 h for Gram stai

124 veloped in-house), were utilized to expedite pyrosequencing data analysis.

125 We apply the method to 454 amplicon pyrosequencing data obtained from a malaria virulence ge

126 Pyrosequencing data revealed majority overlap in differe

127 Pyrosequencing data revealed that the dominant phyla rel

128 Analysis of ultradeep pyrosequencing data sets derived from virus amplicons fr

129 The pyrosequencing data were cross-checked by using the sing

130 In addition, pyrosequencing demonstrated higher levels of methylation

131 Targeted bisulfite pyrosequencing demonstrated that miR-34A was inactivated

132 Error-correcting algorithms for pyrosequences ('denoising') reduced discrepancies in ric

133 Evaluation of pyrosequencing-derived 16S rRNA gene sequences recovered

134 The 16S rRNA gene pyrosequencing did not have sufficient resolution to ide

135 Pyrosequencing errors, consisting mainly of nucleotide i

136 f 16S rRNA-encoding gene sequence reads (454-pyrosequencing) examined viable and total biofilm commun

137 Pyrosequencing experiments validated the cell type-speci

138 terial 16S rRNA genes, we amplified and then pyrosequenced faecal DNA from stool samples.

139 Fresh soils were pyrosequenced for fungal internal transcribed spacer (IT

140 e assessed HVR1 evolution by using ultradeep pyrosequencing for a cohort of treatment-naive, chronica

141 polymerase chain reaction amplification with pyrosequencing for a rapid approach to generate the comp

142 length p17 and p24 gag were generated by 454 pyrosequencing for all pairs near the time of transmissi

143 We employed bacterial fingerprinting and pyrosequencing for microbiome analysis.

144 Our findings confirm the potential of pyrosequencing for quantifying microbial diversity, but

145 umbricus rubellus through utilizing 16S rRNA pyrosequencing for the first time to establish the micro

146 Independent verification by bisulfite pyrosequencing generally confirmed the percentage methyl

147 Pyrosequencing generated more than 150,000 sequences, re

148 Pyrosequencing genotyping and sequencing of the voltage

149 anscriptome profiling and gene-specific cDNA-pyrosequencing have documented that transcriptome shock

150 all and medium CMNs using Sanger sequencing, pyrosequencing, high-resolution melting analysis, and mu

151 Pyrosequencing identified abundant atypical bacteria in

152 Thus, pyrosequencing illustrated that while DB, PRB, and SRB r

153 est were verified by means of locus-specific pyrosequencing in 35 sibling pairs and replicated in an

154 thylation was measured by means of bisulfite pyrosequencing in patients.

155 terval [CI]) for Universal 16S rRNA gene PCR/pyrosequencing included sensitivity of 77.8% (69.5 to 84

156 Pyrosequencing indicated biofilm communities were all si

157 lly available high-throughput locus-specific pyrosequencing instruments allow for the simultaneous an

158 motives, which cannot be evaluated by these pyrosequencing instruments due to software limitations.

159 uency by single-genome amplification and 454 pyrosequencing, (iv) virion-associated Env content, and

160 In summary, shotgun pyrosequencing metagenomic analyses of agricultural dust

161 In this study, we performed shotgun pyrosequencing metagenomic analyses of DNA from dusts fr

162 alignment showed that 19% or 62% of shotgun pyrosequencing metagenomic DNA sequence reads from swine

163 Mutational status was determined by pyrosequencing method, allelic discrimination, or Sanger

164 Pyrosequencing methylation and HPV genotyping assays wer

165 Baseline pyrosequencing methylation and human papillomavirus (HPV

166 the Sanger sequencing, the 'next generation' pyrosequencing, microarrays, quantitative PCR, and the r

167 Culture-independent pyrosequencing microbiome analysis was used to examine t

168 However, out of 23 candidates tested by pyrosequencing, none could be confirmed.

169 Repeat pyrosequencing of 13 specimens showed reproducible detec

170 Pyrosequencing of 16S rDNA is widely used to study micro

171 Pyrosequencing of 16S rDNA was used to study changes in

172 Analyses of community composition by pyrosequencing of 16S rRNA and hoxH genes indicate that

173 Fecal and ileal microbiota were analyzed by pyrosequencing of 16S rRNA genes and enumeration of sele

174 In total, 24 samples were examined using pyrosequencing of 16S rRNA genes and various chemical pr

175 Pyrosequencing of 16S rRNA genes results revealed an age

176 Quantitative PCR (qPCR) and pyrosequencing of 16S rRNA genes was used to quantify th

177 ng six marine lakes and one ocean site using pyrosequencing of 16S rRNA genes.

178 Pyrosequencing of 16S rRNA transcripts from the indigeno

179 By combining pyrosequencing of 18S ribosomal RNA genes with data on m

180 acterization was performed by using 16S rDNA pyrosequencing of 872 nasal swabs collected biweekly fro

181 We demonstrate via 16S rRNA gene pyrosequencing of 922 specimens from 58 subjects that th

182 We used 454 pyrosequencing of a normalized adult cDNA library and de

183 In contrast, SSCP and pyrosequencing of an unrelated single SNP (rs1835740:C >

184 bacterial communities were characterized by pyrosequencing of barcoded 16S rRNA genes and clustered

185 DNA methylation was assessed by pyrosequencing of bisulfite converted DNA and methylated

186 independent sample set of 558 subjects using pyrosequencing of bisulfite-converted DNA (min P-value <

187 ent case-control participants using targeted pyrosequencing of bisulfite-converted DNA.

188 Utilizing extensive transcriptome pyrosequencing of diverse taxa, we established a resolve

189 Direct Sanger sequencing as well as pyrosequencing of environmental isolates demonstrated th

190 Data to support this includes V1-V3 pyrosequencing of formation water and drilling mud, as w

191 and analyzed community composition based on pyrosequencing of fungal internal transcribed spacer (IT

192 Pyrosequencing of genetic marker, COI (cytochrome c oxid

193 geting clades of the xoxF gene, and used 454 pyrosequencing of PCR amplicons obtained from the DNA of

194 DGGE and 454-pyrosequencing of PCR-amplified 16S rRNA genes were used

195 Pyrosequencing of pig caecal 16S rRNA gene amplicons ide

196 MeTIL markers can be determined by bisulfite pyrosequencing of small amounts of DNA from formalin-fix

197 f 86 school age children was analyzed by 454 pyrosequencing of the 16S rRNA gene fragments.

198 200 paired sputum samples from 93 CF adults; pyrosequencing of the 16S rRNA gene was applied to 59 pa

199 Using 454 pyrosequencing of the 16S rRNA gene, we compared bacteri

200 8) and peri-implantitis (n = 6) sites using pyrosequencing of the 16S rRNA gene.

201 ed soil, rhizosphere, and plant roots by 454-pyrosequencing of the 16S rRNA gene.

202 rm and nonfarm children were analyzed by 454-pyrosequencing of the bacterial 16S ribosomal RNA gene.

203 In this work, pyrosequencing of the bacterial 16S rRNA gene was firstl

204 CM fungal communities were identified by 454 pyrosequencing of the fungal internal transcribed spacer

205 DNA methylation levels were assessed by pyrosequencing of the LINE-1 retroelement promoter in DN

206 Pyrosequencing of the PCR amplicons identified a total o

207 Pyrosequencing of the pfcrt gene codon 76 region was per

208 Pyrosequencing of the two feeding groups found that meth

209 Biofilm samples were analyzed by FLX+ pyrosequencing of the V1 to V4 hypervariable regions of

210 ace sediments were used as templates for tag pyrosequencing of the V4 region of the 18S ribosomal DNA

211 or out of season (n = 4) were amplified and pyrosequenced on the 454 GS FLX+ System.

212 amplicons were subjected to high-throughput pyrosequencing on a Roche 454 GS-FLX platform.

213 centage methylation (%5mC) was analysed with pyrosequencing on buccal DNA.

214 tive was to perform whole-genome shotgun 454 pyrosequencing on the same fecal specimens collected in

215 Second, although most of the pyrosequenced operational taxonomic units (OTUs) were di

216 and homozygote mutant genotypes compared to pyrosequencing or Sanger sequencing.

217 In conclusion, the OLA and pyrosequencing performed similarly in the quantification

218 c Gram-negative rods had target-specific PCR/pyrosequencing performed.

219 Pyrosequencing permits accurate quantification of DNA me

220 e for the presence of similar sequences, the pyrosequencing platform correctly classified all 12 inci

221 le-measure incidence assay by implementing a pyrosequencing platform.

222 sity surveys which used PCR or multiplex 454 pyrosequencing platforms showed that the bacterial diver

223 critical for ligation of the OLA probes and pyrosequencing primers, respectively.

224 ight the importance of careful evaluation of pyrosequence processing for using this method to address

225 agnitude either using different criteria for pyrosequence processing or among pyrosequencing, cloning

226 We analyzed, based on pyrosequencing profiling of the biofilm communities in 1

227 e sample processing was included for PCR and pyrosequencing protocols, the molecular approach yielded

228 We developed a pyrosequencing (PSQ) assay including eight subassays for

229 rnia Department of Public Health began using pyrosequencing (PSQ) to detect mutations associated with

230 We described AM fungal communities using 454-pyrosequencing, quantified the proportion of root length

231 own of residence, and experimental plate for pyrosequencing reactions.

232 ified by adenosine triphosphate analysis and pyrosequencing, respectively.

233 ial 16S ribosomal RNA gene was amplified and pyrosequenced, resulting in 370,849 high-quality reads f

234 The pyrosequencing results corroborated our analysis, withou

235 Based on the pyrosequencing results, it appears that clindamycin has

236 actors most likely to affect the accuracy of pyrosequencing results.

237 The 16S rDNA-targeted pyrosequencing revealed a significant change in the comp

238 Pyrosequencing revealed an increase in percentage of Gra

239 Pyrosequencing revealed pronounced diversity co-exists w

240 Pyrosequencing revealed relatively stable bacterial comm

241 Pyrosequencing revealed shared HIV-1 antibody clonal lin

242 However, pyrosequencing revealed that 15/15 (100%) of patients ha

243 16S rRNA gene amplicon pyrosequencing revealed that bacterial communities highl

244 Methylated DNA immunoprecipitation-PCR and pyrosequencing revealed that developmental BPA exposure

245 Pyrosequencing revealed that the population belonging to

246 Community analysis using 16S rRNA pyrosequencing revealed the dominance of Methanosarcina

247 49 subjects with paired specimens tested by pyrosequencing, S. aureus was identified from 11 (22%) a

248 2.0%, and at 125 codons (28%; P < 0.0001) by pyrosequencing sensitive to 0.1%.

249 454 pyrosequencing showed a significant selection for diazot

250 16S rRNA amplicon pyrosequencing showed an increase in the relative abunda

251 expression; promoter methylation analysis by pyrosequencing showed no differences in Bmpr1a, Ptch1, S

252 16S rRNA gene pyrosequencing showed that Gram-positive bacteria affili

253 Pyrosequencing showed that novel strains Peptoniphilacea

254 Operational taxonomic units were pyrosequenced targeting 16S ribosomal RNA and volatile o

255 somal RNA gene libraries sequenced using 454-pyrosequencing targeting the V1 to V3 and V7 to V9 regio

256 Amplicon pyrosequencing targets a known genetic region and thus i

257 g 15 GIST and 17 PPGL) was performed using a pyrosequencing technique and correlated with SDHC gene e

258 Pyrosequencing technology provides an important new appr

259 om DNA analyses of the in situ samples using pyrosequencing technology, we found the highest abundanc

260 gender-matched controls using the bisulphite pyrosequencing technology.

261 ial changes were monitored by amplifying and pyrosequencing the 16 S ribosomal small subunit region.

262 e fecal microbiota from these two species by pyrosequencing the 16S V1-V3 hypervariable regions using

263 We profiled the fecal microbiota by pyrosequencing the gene encoding 16S ribosomal RNA (rRNA

264 ith pine seedlings, and colonized roots were pyrosequenced to detect resistant propagules of ECM fung

265 We used 16S rRNA gene amplification and pyrosequencing to characterize, for the first time to ou

266 We used next generation 454 pyrosequencing to discern the whole-body microbiome of t

267 s were subjected to whole-genome shotgun 454 pyrosequencing to identify both fecal bacterial populati

268 ions by using phylogenic microarrays and 454 pyrosequencing to identify microorganisms and functional

269 We used pyrosequencing to investigate allelic imbalance of Oxtr

270 tersubtype Env recombinants and utilized 454 pyrosequencing to investigate the distribution of over 4

271 eotrichia (class Spirotrichea), by comparing pyrosequencing to Sanger-sequenced clone libraries and m

272 Here we compared the results of PCR/pyrosequencing to those of culture for detecting bacteri

273 ten-eleven translocation-assisted bisulfite pyrosequencing, to quantify FMR1 5mC and 5hmC levels.

274 es, multicolor flow cytometry, and bisulfite pyrosequencing, to study the effects of H pylori on alle

275 The initial PCR/pyrosequencing took approximately 5.5 h to complete or a

276 In this study, we used ultradeep pyrosequencing (UDPS) to map the viral heterogeneity of

277 uencing (NGS), capillary electrophoresis and pyrosequencing under the term 'NGS+' for typing Y-STRs a

278 ive to 2.0%, at 71 codons (16%; P = 0.78) by pyrosequencing using a cutoff value of >/= 2.0%, and at

279 arine sediment cores by quantitative PCR and pyrosequencing using newly designed DEH 16S rRNA gene ta

280 Next-generation pyrosequencing validated the congruence of the potential

281 454-pyrosequencing was able to recover 10 more families of a

282 Pyrosequencing was employed to characterize bacteria and

283 ent for detection of bacteria by culture and pyrosequencing was good for aerobic bacteria such as P.

284 While pyrosequencing was more sensitive, detection of mutants

285 Targeted bisulfite pyrosequencing was performed on a validation cohort of 3

286 ity structure appeared to remain stable when pyrosequencing was performed on samples that were not su

287 Pyrosequencing was used for DNA methylation quantificati

288 454 pyrosequencing was used to assign operational taxonomic

289 s prior to DNA extraction, and 16S rRNA gene pyrosequencing was used to characterize their bacterial

290 Pyrosequencing was used to confirm the imprinting status

291 Pyrosequencing was used to evaluate deviation from monoa

292 In this study, 454 pyrosequencing was used to identify the bacteria associa

293 Culture-independent high-density Roche 454 pyrosequencing was used to survey the distal gut microbi

294 Next-generation sequencing (454 pyrosequencing) was performed to reveal the CPB transcri

295 Using pyrosequencing we followed up the two most differentiall

296 Using pyrosequencing, we elucidated how important phylotypes o

297 DNA extraction and pyrosequencing were done on several core sections in ord

298 tive polymerase chain reaction (PCR) and 454 pyrosequencing were used to analyze bacterial 16S riboso

299 ferent spot positions, quantitative PCR, and pyrosequencing, were used to confirm the role of the can

300 during monochloramine disinfection using PMA-pyrosequencing, while the community structure appeared t