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1 ditions, unlike Escherichia coli, which uses pyruvate formate lyase.
2 ted from formate, which was generated by the pyruvate formate-lyase.
3 specific abstraction of a hydrogen atom from pyruvate formate-lyase.
4 H atom abstraction from the buried G(734) of pyruvate formate-lyase.
5 a catalytically essential glycyl radical on pyruvate formate-lyase.
6 l radical formation on the native substrate, pyruvate formate-lyase.
7 zyme A from the labeled pyruvate, formed via pyruvate-formate lyase.
8 s in anaerobic ribonucleotide reductases and pyruvate-formate lyases.
11 PFL requires the activities of two enzymes: pyruvate formate lyase activating enzyme (coded by pflA)
12 edure, similar to what has been reported for pyruvate formate lyase activating enzyme (PFL-AE), was e
14 ctivation of pyruvate formate-lyase (PFL) by pyruvate formate-lyase activating enzyme (PFL-AE) involv
17 Here, we probe the reaction of the GRE-AE pyruvate formate-lyase activating enzyme (PFL-AE) with t
19 S-adenosyl-l-methionine (SAM) bound forms of pyruvate formate-lyase activating enzyme are reported.
20 rt in which photoinduced ET in the RS enzyme pyruvate formate-lyase activating enzyme cleaved the S-C
22 reaction catalyzed by the radical SAM enzyme pyruvate formate-lyase activating enzyme on a peptide su
24 adical S-adenosyl-l-methionine (SAM) enzyme, pyruvate formate lyase-activating enzyme (PFL-AE), both
26 substrate-free and substrate-bound forms of pyruvate formate-lyase-activating enzyme, the first stru
29 g the oxygen-sensitive glycyl radical enzyme pyruvate formate lyase and a phosphate acyltransferase t
31 e radical and that of the glycyl radicals of pyruvate formate-lyase and anaerobic ribonucleotide redu
32 acid synthase and the activating enzymes for pyruvate formate-lyase and anaerobic ribonucleotide redu
33 ce similarity to the glycyl radical enzymes, pyruvate formate-lyase and anaerobic ribonucleotide redu
34 between the activase and the G(734) loop of pyruvate formate-lyase and provide a structural basis fo
35 ns has other enzymatic activities, including pyruvate formate-lyase and pyruvate dehydrogenase, for p
37 :ferredoxin oxidoreductase PFR1, which, like pyruvate:formate lyase and HYDA1, is localized in the ch
38 c ribonucleotide reductase, biotin synthase, pyruvate formate lyase, and cobalamin-dependent methioni
40 he pyruvate dehydrogenase complex (PDHC) and pyruvate formate-lyase are considered the primary routes
45 eversed pyruvate ferredoxin oxidoreductase / pyruvate-formate-lyase-dependent (rPFOR/Pfl) pathways.
47 n pathogenic Salmonella, and we identify the pyruvate formate-lyase-encoding pflB gene as a key drive
49 beled formate from pyruvate by the action of pyruvate-formate lyase is also consistent with the label
51 his analysis include the identification of a pyruvate-formate lyase-like operon, likely to be express
52 ynthase, anaerobic ribonucleotide reductase, pyruvate formate lyase, lysine 2, 3-aminomutase, and lip
53 ate, ethanol, and acetate are generated by a pyruvate:formate lyase pathway similar to Escherichia co
54 er was similar to what has been observed for pyruvate formate lyase (PFL) and anaerobic ribonucleotid
57 ion, we studied the role of the pneumococcal pyruvate formate lyase (PFL), a key enzyme in mixed-acid
58 t the Escherichia coli glycyl radical enzyme pyruvate formate lyase (PFL), which catalyzes the anaero
59 e action of pyruvate decarboxylase (PDC) and pyruvate formate lyase (PFL)-enzymes that catalyze the d
60 ichia coli unable to synthesize a functional pyruvate formate-lyase (PFL) are severely impaired in th
61 e-lyase-activating enzyme (PFL-AE) activates pyruvate formate-lyase (PFL) by generating a catalytical
64 nerated from the glycyl radical of activated pyruvate formate-lyase (PFL) during the process of oxyge
67 A as a substrate and generates pyruvate, and pyruvate-formate lyase (PFL) converting pyruvate to form
68 lation was shown to depend upon two enzymes, pyruvate:formate lyase (PFL) and pyruvate:ferredoxin oxi
69 hree Chlamydomonas mutants disrupted for the pyruvate formate lyase (PFL1) gene; the encoded PFL1 pro
70 ate that the normally soluble dimeric enzyme pyruvate formate-lyase (PflB), which is responsible for
71 ically essential glycyl radical on G(734) of pyruvate formate-lyase via the direct, stereospecific ab