ライフサイエンスコーパス: qPCR

1 qPCR and immunohistochemistry confirmed gene and protein

2 qPCR and immunohistochemistry verified the significant u

3 qPCR and western blotting were used to assess ion channe

4 qPCR confirmed increased expression of GDPD1 and increas

5 (T) ] values for the Rep 529-1 and Rep 529-2 qPCRs were lower than those for the B1 qPCR [P < 0.001 a

6 The performances of the 3 qPCRs were also compared according to the genotypes of t

7 ata revealing that the efficiency of Rep 529 qPCR does not depend on the genotype of T. gondii isolat

8 R (qPCR) with those of two different Rep 529 qPCRs performed on 111 samples in two different laborato

9 A qPCR test for the detection of the highly repetitive Tso

10 umoniae (S. pneumoniae), respectively; and a qPCR assay targeting the tuf gene for detection and quan

11 The currently used molecular test is a qPCR assay targeting the ITS1 rDNA region (ITS1) of Ac.

12 This study characterized the effects of a qPCR data analysis using the sample PCR efficiencies (th

13 two patient tumour tissue samples through a qPCR instrument and finally piloted on an ISFET enabled

14 AcanR3990 qPCR did not cross-react in five CSF from patients with

15 AcanR3990 qPCR failed to amplify genomic DNA from the other relate

16 These results suggest AcanR3990 qPCR assay is highly sensitive and specific with potenti

17 m varied geographic locations, the AcanR3990 qPCR detected the presence of Ac in 49/49 ITS1 confirmed

18 Using immunostaining, western blot and qPCR methods, we firstly identified that ITCH was expres

19 Flow cytometry and qPCR further analyzed ex vivo the glomerular leukocyte i

20 Our results suggest ELISA and qPCR did not have strong diagnostic agreement, despite F

21 luated the diagnostic agreement of ELISA and qPCR, and whether differences in their diagnostic accura

22 two FFV diagnostic tests available-ELISA and qPCR-as well as the prevalence of FFV in a large cohort

23 r collection, filtration, DNA extraction and qPCR analysis, which allowed for real-time remote report

24 rred from MitoTimer protein fluorescence and qPCR.

25 enta assessment via immunohistochemistry and qPCR.

26 Microarray and qPCR analysis of gastrocnemius muscle RNA revealed that

27 apparent ophidiomycosis (lesions present and qPCR positive), and the best models predicting qPCR resu

28 anslating ribosome affinity purification and qPCR was used to compare mRNA expression of nrg1,2,3,4 a

29 Although improved access to screening and qPCR increased the number of infants diagnosed with cong

30 were examined by Western blot, RNA-seq, and qPCR.

31 During the selection, we applied qPCR-based analyses to evaluate the ssDNA pool size and

32 aration for common biological assays such as qPCR.

33 of 3 pg/muL of DNA, similar to the available qPCR kits, is achieved, but it is cheaper, easier and av

34 6 (1.7%) of the 1554 recruits with available qPCR results tested positive on day 14.

35 529-2 qPCRs were lower than those for the B1 qPCR [P < 0.001 and P < 0.01, respectively]).

36 ates and that, in fact, it is superior to B1 qPCR.

37 rray Card that compartmentalised probe-based qPCR for 32 enteropathogens.

38 Echocardiography, Western blotting, qPCR, immunohistochemistry, immunofluorescence, and tran

39 n virus, coxsackievirus B5, by applying both qPCR- and culture-based methods to measure inactivation

40 samples that gave Ct values below 22 on both qPCR assays were positive on the Pan-Lassa RDT.

41 Analysis by qPCR showed novel DNA repeat families were comparatively

42 g ACTA2, TGFBR1, and TGFBR2 were analyzed by qPCR.

43 he HER2 gene in the tumor tissue assessed by qPCR (but not by FISH) have significantly more often a h

44 mRNA and protein expression were assessed by qPCR, flow cytometry, Western blotting, and immunofluore

45 nts had undetectable cccDNA when assessed by qPCR, RCA and ddPCR assays detected cccDNA in all-but-on

46 l enhancer RNA at this locus was assessed by qPCR.

47 MMP9 expression was confirmed by qPCR and immunocytochemistry of odontoclasts located in

48 nd their discriminatory ability confirmed by qPCR.

49 atients, while A fumigatus was detectable by qPCR in 46%.

50 ty-one serotypes/serogroups were detected by qPCR compared to 14 by CCBM.

51 kel cell polyomavirus (MCV) were detected by qPCR in 49% and 19% of cases, respectively.

52 increased during 2 months after diagnosis by qPCR and remained on a plateau for the remainder of the

53 abundance of Prevotellaceae, as evidenced by qPCR with 16S rRNA primers.

54 d insect access and confirmed as HLB free by qPCR.

55 eviously tested positive for P. jirovecii by qPCR assay and 50 control samples (retrospective part),

56 ase, but not estrogen receptors, measured by qPCR changes across the reproductive cycle.

57 d three immune cell markers were measured by qPCR in the prefrontal cortex from 37 people with schizo

58 ase-expressing UP strain, and post-mortem by qPCR and bacterial titration.

59 screened for the presence of pneumococci by qPCR targeting lytA and piaB.

60 visible by microscopy in 70% and present by qPCR in 86% of patients, while A fumigatus was detectabl

61 MiR-34a was quantified by qPCR, and rs2666433 (A/G) genotyping was performed by Ta

62 ion factors and cytokines were quantified by qPCR.

63 etected in eight (44.4%) baseline samples by qPCR and the number of positives declined post-treatment

64 idermidis detected by WGS or DNA from TTV by qPCR in ocular fluids is associated with worse outcomes

65 of fragments that would be unrecoverable by qPCR.

66 endogenous control genes for bladder cancer qPCR.

67 Single-cell qPCR revealed that these genes were also differentially

68 hich we validated by in situ and single-cell qPCR.

69 H2AX by chromatin immunoprecipitation (ChIP)-qPCR in order to uncover the mechanisms by which Mec1(AT

70 ChIP-qPCR experiments revealed increased beta-catenin recruit

71 ChIP-qPCR unraveled that YAP-5SA overexpression increased bin

72 o protein-protein interaction analysis, ChIP-qPCR and EMSA were performed and suggested that HSI2 and

73 ChIP-seq and ChIP-qPCR provided evidence that the CDK inhibitor directly i

74 (TM6SF2), the latter being confirmed by ChIP-qPCR.

75 infections were identified through clinical qPCR testing performed as a result of daily symptom moni

76 correlated (R(2) > 0.99, p < 0.001) to a CMV-qPCR assay conducted on DNA isolated from whole blood sa

77 and composition of the microbial community (qPCR) in digesta were determined.

78 Concomitantly, qPCR analysis showed that curing enhanced upregulation o

79 performing the experiments on a conventional qPCR instrument (n = 41).

80 s from 31 patients were examined by culture, qPCR, whole genome sequencing, serotyping, and reverse t

81 The developed qPCR assay presented a detection limit of 10.14 fg/react

82 n serum viral load measurements by different qPCR assays.

83 A recently proposed method for estimating qPCR amplification efficiency E analyzes fluorescence in

84 Fast24-qPCR with repeat testing had a sensitivity of 87.3% (95%

85 We found Fast24-qPCR to have a sensitivity of 96.7% (95% confidence inte

86 urther, we independently repeated the Fast24-qPCR test on positive samples, increasing stringency by

87 e performance of the existing Warwick Fast24-qPCR test and its modified version based on a high-throu

88 With Fast24-qPCR, we provide a social-group-level test with sufficie

89 Fast96-qPCR requires further optimization.

90 igh-throughput DNA extraction method (Fast96-qPCR).

91 Finally, qPCR was also used to validate two deletion events in li

92 Five qPCR assays were evaluated: the universal GenBac3, human

93 way to identify endogenous control genes for qPCR analysis of formalin-fixed paraffin-embedded (FFPE)

94 easy, informed choice of reference genes for qPCR transcriptomic studies.

95 of open-source automated liquid handlers for qPCR, bioplotting, and other bioinstrumentation applicat

96 k postinfection compared with 1 to 32 mo for qPCR.

97 or a standardized data analysis protocol for qPCR MST assays for interlaboratory consistency and comp

98 pped with real-time PCR systems required for qPCR diagnostics.

99 eeds and positional resolutions required for qPCR.

100 ncies in gene expression values derived from qPCR experiments in the presence of non-detects, providi

101 fter 3 weeks and visible coverage and fungal qPCR concentrations were correlated (R(2) = 0.55).

102 Gram stain Nugent scoring and 16S rRNA gene qPCR and HiSeq sequencing.

103 Moreover, by comparing with SYBR Green qPCR, we further validated the feasibility of the triple

104 falciparum patients were more likely to have qPCR detected P. falciparum infections (22.0%, 9/41) com

105 and HLB as case study, we monitored healthy (qPCR-negative) in-field grown citrus trees and compared

106 e BRD4 and gammaH2AX ChIP-Seq with R-loop IP qPCR reveals that BRD4 inhibition leads to accumulation

107 th 15/73 samples previously negative by ITS1 qPCR despite strong clinical suspicion for angiostrongyl

108 ex PJA with that of established P. jirovecii qPCR assays.

109 le to those for three different P. jirovecii qPCR assays.

110 ample preparation times comparable to manual qPCR.

111 uals), whereas current methods of molecular (qPCR) and visual detection failed to contribute to the s

112 the methylation status of the TSDR, our MSRE-qPCR results were within 5% on average for all samples w

113 ve restriction enzyme-quantitative PCR (MSRE-qPCR) and observed that multiple genes of the IL-12/IFN-

114 ntage (>70%) of Tregs, reinforcing that MSRE-qPCR can be completed in less time than other methods wi

115 os of Tregs and non-Tregs, we show that MSRE-qPCR can distinguish the methylation status of the TSDR

116 MTT, qPCR and immunoblotting assays tested the effects of cot

117 singleplex and non-matrix scoring multiplex qPCR assays utilize a hydrolysis probe, providing sensit

118 This multiplex qPCR assay could support early clinical diagnosis and tr

119 r for CSF (94.4% of those cured had negative qPCR results) than for plasma (86.7% of those cured test

120 e a significant risk factor, whereas neither qPCR nor ELISA identified sex to be a risk factor.

121 ELISA, but not qPCR, identified age to be a significant risk factor, wh

122 NA pool size and remelting curve analysis of qPCR amplicons to monitor changes in pool diversity and

123 established, approaches for the analysis of qPCR data continue to improve.

124 An important aspect of qPCR data that has been largely ignored is the presence

125 ements in performance and reproducibility of qPCR, LAMP, and RT reactions.

126 very low bacteremia, optimal sensitivity of qPCR for these rickettsioses will require use of larger

127 Our study highlights that combined use of qPCR and ELISA for FFV may enhance estimates of the true

128 Recently, use of qPCR has challenged these observations.

129 In conclusion, use of qPCR suggests that pneumococcal carriage in Portuguese e

130 mps were generated, tested, and validated on qPCR for the detection of the antimicrobial resistance g

131 The odds that an RDT or qPCR was positive was reduced by more than 99% (OR, 0.00

132 e odds that a rapid diagnostic test (RDT) or qPCR was positive was reduced by 89% (odds ratio [OR], 0

133 Our qPCR assay utilized repetitive LINE-1 elements specific

134 gned a multiplex real-time quantitative PCR (qPCR) assay to detect SFGR, TGR, O. tsutsugamushi, and i

135 Quantitative PCR (qPCR) assays are the gold standard for diagnosis of Pneu

136 Pan-LASV RDT to available quantitative PCR (qPCR) assays during the 2018 LF outbreak in Nigeria.

137 B probe-based fluorescence quantitative PCR (qPCR) assays to perform both qualitative and quantitativ

138 ples positive by nanoliter quantitative PCR (qPCR) for V. cholerae (n = 78/849), the odds that a rapi

139 se with RNA-sequencing and quantitative PCR (qPCR) results in eWAT, the mRNA levels of several adipok

140 er, practices learned from quantitative PCR (qPCR) that promote assay robustness and wide-ranging uti

141 riction enzymes (MSRE) and quantitative PCR (qPCR) to determine the methylation status of the TSDR.

142 Rapid diagnostic test and quantitative PCR (qPCR) were used for the detection of infections that wer

143 compared the results of B1 quantitative PCR (qPCR) with those of two different Rep 529 qPCRs performe

144 hrough field surveys using quantitative PCR (qPCR), with the conclusion that it was the most likely v

145 lect bacteria for targeted quantitative PCR (qPCR).

146 low identical to real-time quantitative PCR (qPCR).

147 Quantitative real-time PCR (qPCR) and Western blot analyses confirmed that the level

148 ion of all bacterial species; real-time PCR (qPCR) assays targeting the femA or lytA gene for detecti

149 le and multiplex quantitative real-time PCR (qPCR) assays that can accurately detect and identify the

150 ed PCR/analytically validated real-time PCR (qPCR) with bidirectional sequencing.

151 idation by using quantitative real-time PCR (qPCR), all of which were successfully confirmed.

152 applications of quantitative real-time PCR (qPCR), next-generation sequencing (NGS) and bioinformati

153 ory-based instruments such as real-time PCR (qPCR).

154 riptase PCR, and quantitative real-time PCR (qPCR).

155 ails of sheep through transcriptome, RT-PCR, qPCR, and Western blot analyses.

156 or pathogenic disease by gold standard (PCR, qPCR, RT-qPCR) methods, therefore, alternative methods h

157 e had symptoms in the week before a positive qPCR test.

158 CR positive), and the best models predicting qPCR result and ophidiomycosis category included individ

159 handle, and high-throughput TaqMan-MGB probe qPCR assay to perform both qualitative and quantitative

160 reliable detectable ability of TaqMan probe qPCR assays without cross-reactivity upon probes combina

161 lidated the feasibility of the triplex-probe qPCR assay for the quantitative detection of mtDNA copy

162 ough quantitative polymerase chain reaction (qPCR) analysis, we found that our inertial sorting appro

163 plex quantitative polymerase chain reaction (qPCR) assay using TaqMan probe was developed to discrimi

164 itative real time polymerase chain reaction (qPCR) data are normalised using endogenous control genes

165 Quantitative polymerase chain reaction (qPCR) is the technique of choice for quantifying gene ex

166 sing quantitative polymerase chain reaction (qPCR) of the nifH (marker gene used to identify biologic

167 Quantitative polymerase chain reaction (qPCR) was performed for specific pathogens and whole-gen

168 ence quantitative polymerase chain reaction (qPCR) was performed.

169 ISA, quantitative polymerase chain reaction (qPCR), and immunohistochemistry.

170 sing quantitative polymerase chain reaction (qPCR), and lastly, quantified baseline extracellular DA

171 and quantitative Polymerase Chain Reaction (qPCR), we measured protein and mRNA levels of a panel of

172 y or quantitative polymerase chain reaction (qPCR), were included in the study.

173 with quantitative polymerase chain reaction (qPCR).

174 sing quantitative polymerase chain reaction (qPCR).

175 s of quantitative polymerase-chain-reaction (qPCR) assay of nares swab specimens obtained between the

176 by a quantitative polymerase-chain-reaction (qPCR) assay.

177 RT-qPCR also showed enhanced stem cell and axonal guidance

178 RT-qPCR analyses in transgenic lines revealed extremely low

179 RT-qPCR analysis of a sampling of tissues from the head dem

180 RT-qPCR demonstrated viral replication in salmon brains up

181 RT-qPCR revealed that the telencephalon and thalamus were c

182 a subgroup analysis, RT-RPA detected 9/10 RT-qPCR-positive samples, while loop-mediated isothermal am

183 plicate commercially available SARS-CoV-2 RT-qPCR test kits and backfill pipeline shortages.

184 EV-associated miRNAs were identified by a RT-qPCR-based profiling.

185 on, we performed transcriptomic analyses, RT-qPCR, CRISPR-Cas9 modification of human intestinal enter

186 Nile Red efflux assays and RT-qPCR analysis suggest ospemifene interferes directly wit

187 d of the study, the NAc was dissected and rt-qPCR methods were used to estimate CRF overexpression an

188 The validated RNA extraction and RT-qPCR platform has been installed in NHS diagnostic labs,

189 standard, we validate RNA extraction and RT-qPCR workflows as well as two detection assays based on

190 ques for CTV detection include RT-PCR and RT-qPCR.

191 ubjected to RNA in situ hybridization and RT-qPCR.

192 s cDNA synthesis, gene amplification, and RT-qPCR.

193 able element (TE) RNA expression, such as RT-qPCR and RNA-seq, that do not distinguish between TEs ex

194 AVM-BECs were determined by Western blot, RT-qPCR (quantitative reverse transcription polymerase chai

195 al cases had CHIKV infection confirmed by RT-qPCR (52.9%), viral antigen (41.1%), and/or specific-IgM

196 SARS-CoV-2 was detected by RT-qPCR and viral culture; the limit of detection for cultu

197 , a total of 162 NHP were YFV positive by RT-qPCR and/or immunohistochemistry, being 22 Callithrix-sp

198 Stool specimens were tested by RT-qPCR for GI and GII noroviruses and subsequently genotyp

199 s2), as well as knockdown confirmation by RT-qPCR of two other endogenous genes.

200 E circRNAs have been further validated by RT-qPCR using divergent primers spanning back-splicing junc

201 RSV infection was confirmed by RT-qPCR with any positive value (ITT-I population) or RSV R

202 s observed in FIR-responsive CAD ECFCs by RT-qPCR.

203 ALP, Osterix, and MMP13 were measured by RT-qPCR.

204 Combining RT-qPCR with microbiological assays (colony and surface pel

205 -2 RNA by traditional clinical diagnostic RT-qPCR that included an RNA extraction.

206 The direct RT-qPCR approach correctly identified 92% of a reference se

207 be detected from NP samples via a direct RT-qPCR assay that omits the RNA extraction step altogether

208 dies on validation of reference genes for RT-qPCR analysis in A. eugenii limits the investigation of

209 capsidiol-related genes were selected for RT-qPCR analysis, two 5-epi-aristolochene synthase (CA12g05

210 results, specific genes were selected for RT-qPCR evaluation using species-specific primers.

211 heir usage as reference gene products for RT-qPCR experiments, when quantifying mRNA levels in human

212 saliva and induced sputum are desired for RT-qPCR test or other early detection technologies.

213 An independent RT-qPCR experiment on seven genes in twelve cancer cell lin

214 lification and detection on an integrated RT-qPCR-CE (capillary electrophoresis (CE)) microfluidic ch

215 developed a fully automated microfluidic RT-qPCR system for rapid quantitative detection of HCV RNA

216 required reagents, we devised a multiplex RT-qPCR assay to detect SARS-CoV-2 in a single reaction.

217 Our novel multiplex RT-qPCR improves upon current single diagnostics by saving

218 le threshold (Ct) values of our multiplex RT-qPCR were comparable to those obtained by the single ass

219 re consistently detected by the multiplex RT-qPCR.

220 From our comparisons of RT-qPCR analytical efficiency and sensitivity, we show that

221 s that required 30-39 threshold cycles of RT-qPCR to achieve a positive detection.

222 RNA-to-DNA reverse transcription step of RT-qPCR with a rapid nucleic-acid-template-dependent DNA ch

223 from a CDC-approved quantitative RT-PCR (RT-qPCR) assay performed in a state testing lab, and superi

224 t quantitative reverse transcription PCR (RT-qPCR) assay recommended for severe acute respiratory syn

225 l quantitative reverse transcription-PCR (RT-qPCR) assays are being used by clinical, research and pu

226 Reverse transcriptase quantitative PCR (RT-qPCR) confirmed the presence of viral RNA in formalin-fi

227 s assessed by real time quantitative PCR (RT-qPCR) or western blots.

228 , reverse transcriptase quantitative PCR (RT-qPCR), and NGS.

229 detect the virus by quantitative RT-PCR (RT-qPCR), the most robust, sensitive, and specific assay cu

230 evelop two versions of a unique five plex RT-qPCR test, termed CoV2-ID, that allows the detection of

231 enic disease by gold standard (PCR, qPCR, RT-qPCR) methods, therefore, alternative methods have been

232 transcription-polymerase chain reaction (RT-qPCR) after 3, 6, or 24 hours of IL-1beta stimulation.

233 tive real-time polymerase chain reaction (RT-qPCR) is widely used for mRNA quantification.

234 n-quantitative polymerase chain reaction (RT-qPCR) to detect the purified SARS-CoV-2 RNA.

235 transcription polymerase chain reaction (RT-qPCR)) and infectivity (TCID(50)).

236 e-quantitative polymerase chain reaction (RT-qPCR), 14 of which resulted in genome assembly.

237 se - real-time polymerase chain reaction (RT-qPCR).

238 transcription-polymerase chain reaction (RT-qPCR).

239 n quantitative polymerase chain reaction (RT-qPCR).

240 the feasibility of establishing a robust RT-qPCR assay at approximately 10% of the cost of equivalen

241 Chest computerized tomography (CT) scan, RT-qPCR, lateral flow immunochromatographic strip (LFICS) f

242 We show RT-qPCR and detailed phenotypic evidence of our method succ

243 e in fluorescence intensity with standard RT-qPCR (Pearson coefficients > 0.7 for the N1, N2 and RPP3

244 t step toward establishing a standardized RT-qPCR analysis in Cryptocercus.

245 , were selected as the candidates for the RT-qPCR analysis.

246 y same assay in a clinical lab, where the RT-qPCR assay exhibited multiple invalid or inconclusive re

247 sitivity and 100% specificity compared to RT-qPCR with a sample-to-answer time of 50 min.

248 ntitative PCR with reverse transcription (RT-qPCR) typically involves bulky instrumentation in centra

249 In this study, we used RT-qPCR, RNA sequencing, pathway, upstream regulator, and h

250 promising alternatives to currently used RT-qPCR-based tests.

251 on of SARS-CoV-2 in patient samples using RT-qPCR, CRISPR-Cas13a, and RT-LAMP.

252 :C), house dust mite (HDM) or IL-33 using RT-qPCR, Luminex and live cell imaging.

253 valuated in human gingival biopsies using RT-qPCR.

254 mples (4.3%) with microfilaridermia using RT-qPCR.

255 sed miRNAs and mRNAs were validated using RT-qPCR.

256 related markers in hDFC was analysed with RT-qPCR.

257 In silico design of a more sensitive qPCR assay was performed based on tandem repeats predict

258 Specific qPCR tests targeting S. aureus and S. pneumoniae did not

259 rRNA gene sequencing or L. murinus-specific qPCR of DNA from total organ homogenates vs.broncho alve

260 his study aimed to design a species-specific qPCR assay, based on klap1 gene, suitable for C. acutatu

261 quantitative polymerase chain reaction (SyG-qPCR) assay were combined to generate a ready-to-use kit

262 ) and Mycoplasma penetrans (MP) for targeted qPCR.

263 In targeted qPCR assays it is typically performed with respect to pr

264 his association was confirmed using targeted qPCR and was independent of infant carriage of P. copri.

265 isease and whether RS is more sensitive than qPCR, the "golden standard" in pathogen diagnostics?

266 te statistical analysis, we also showed that qPCR-negative plants exhibited HLB-specific spectral cha

267 The qPCR tests targeting S. aureus and S. pneumoniae gave ea

268 he clinical diagnosis, the CST assay and the qPCR method reached a sensitivity of 87.82% and 94%, res

269 We then applied the qPCR-based method to establish a UV(254) inactivation cu

270 ve for O. ophiodiicola DNA, and 77.8% of the qPCR positive individuals had skin lesions.

271 s, determination of bacterial load using the qPCR test targeting the tuf gene could help evaluation o

272 Application of these qPCR- and sequencing-based detection assays will provide

273 alyses of POLD3 were conducted via real time qPCR to determine when and in what tissues the gene is e

274 as measured from saliva samples by real-time qPCR at baseline and UPF consumption was collected using

275 rial biogenesis were quantified by real-time qPCR.

276 investigated using zymography and real-time qPCR.

277 nts (n = 12) by flow cytometry and real-time qPCR.

278 in Caco-2 cells were evaluated by real-time qPCR.

279 rmance for the diagnosis of PCP, compared to qPCR assays.

280 ore sensitive diagnostics of HLB compared to qPCR.

281 ive and retrospective samples and subject to qPCR using TaqMan human endogenous control arrays or sin

282 ns with a somewhat lower sensitivity towards qPCR that can be classified as "good."

283 Traditional qPCR analysis methods typically rely on paired designs.

284 ncing, serotyping, and reverse transcription qPCR.

285 The authenticity of these two qPCR products was confirmed by DNA sequencing analysis,

286 ho did not volunteer for the study underwent qPCR testing only on day 14, at the end of the quarantin

287 reverse transcription-quantitative PCR (uRT-qPCR) using nucleic acid extracts from blood samples (n

288 rials, the positive percent agreement to uRT-qPCR was 90% (95% confidence interval [CI], 80 to 96).

289 is particularly useful in settings where uRT-qPCR is difficult to implement.

290 SPP1 mRNA expression was analysed using qPCR (n = 100) and OPN protein by immunohistochemistry (

291 channels in rodent and primate brains using qPCR, in situ hybridization, and immunocytochemical sing

292 f VEGF in the cornea was quantified by using qPCR.

293 swabbed to detect O. ophiodiicola DNA using qPCR.

294 way was studied at transcription level using qPCR.

295 vestigated the absolute bacterial load using qPCR: hemolymph samples contained 2784 +/- 339 bacteria/

296 te load in slit aspirate was monitored using qPCR.

297 (23.4%) samples with microfilaridermia were qPCR-positive.

298 odiicola in five of the 22 species that were qPCR positive.

299 Further analysis with qPCR, flow cytometry and ELISA experiments revealed that

300 transcriptomic analysis in conjunction with qPCR, we find that that MR1A and MR1B transcripts are wi