ライフサイエンスコーパス: quantitative PCR

1 ased assay (Legionella pneumophila only) and quantitative PCR.

2 ssed at 4- and 10-weeks post-infection using quantitative PCR.

3 cluding SIGLEC1, a result corroborated using quantitative PCR.

4 munohistochemistry and reverse transcription-quantitative PCR.

5 rodissection combined with DNA microarray or quantitative PCR.

6 opposite inflammatory states was analyzed by quantitative PCR.

7 Organism load was determined by quantitative PCR.

8 assessed with histology, flow cytometry, and quantitative PCR.

9 opy number, which is quantified by real-time quantitative PCR.

10 Participants' aTLs were measured by using quantitative PCR.

11 on using microsatellites and allele-specific quantitative PCR.

12 wabs were taken daily to monitor shedding by quantitative PCR.

13 ffer systems for their inhibitory effects on quantitative PCR.

14 resentative mouse model were completed using quantitative PCR.

15 e results using targeted techniques, such as quantitative PCR.

16 iglec-7 was quantified by flow cytometry and quantitative PCR.

17 c-8 was assessed by using flow cytometry and quantitative PCR.

18 enes in the small intestine were analyzed by quantitative PCR.

19 (PKDL) or venous blood (VL) was processed by quantitative PCR.

20 NA) in cerebrospinal fluid were amplified by quantitative PCR.

21 R1, and PTK2 were determined using real-time quantitative PCR.

22 AVT, in situ hybridization for avt-mRNA, and quantitative PCR.

23 termined by ELISA, cytometric bead array, or quantitative PCR.

24 off-target genes using reverse transcription quantitative PCR.

25 ears 15, 25) were measured in whole blood by quantitative PCR.

26 (IDO) were analyzed using flow cytometry and quantitative PCR.

27 n the Huntington's gene of genomic DNA using quantitative PCR.

28 two distinct antibodies and MCPyV DNA using quantitative PCR.

29 tissue levels via time-lapse microscopy and quantitative PCR.

30 or harboring HDV RNA detectable by real-time quantitative PCR.

31 was measured by using standardized real-time quantitative PCR.

32 analyzed by 16S rRNA amplicon sequencing and quantitative PCR.

33 e-mount fluorescence staining, and real-time quantitative PCR.

34 human beta-cells was performed by real-time quantitative PCR.

35 ce predicted to be 3 molecules or higher for quantitative PCR.

36 Leptin receptor (Ob-R) was evaluated by quantitative PCR.

37 ld more than the other isoforms by real-time quantitative PCR.

38 /mL or IU/mL, respectively, as determined by quantitative PCR.

39 g, respectively) was quantified by real-time quantitative PCR.

40 absolute S. aureus abundance was measured by quantitative PCR.

41 ential expression patterns were confirmed by quantitative PCR.

42 nt in PBMCs from healthy donors by real-time quantitative PCR.

43 as 1 Pf/uL blood using reverse transcription quantitative PCR.

44 e tested for HHV-6 viremia using a real-time quantitative PCR.

45 -stimulated gene expression were measured by quantitative PCR.

46 ression was measured using microarray and RT quantitative PCR.

47 ciated enzymes is a promising alternative to quantitative PCR.

48 d using microarray and reverse transcriptase quantitative PCR.

49 by immunosorbent assay, Western blotting and quantitative PCR.

50 of genogroups I, II, and IV] were tested by quantitative PCR.

51 signature was further evaluated by real-time quantitative PCR.

52 n brain, spleen, and liver were confirmed by quantitative PCR.

53 timulation and subsequent RNA sequencing and quantitative PCR.

54 t generation sequencing and verified using a quantitative PCR.

55 (n = 329) arms for 34 enteropathogens using quantitative PCR.

56 (mucin 5B) polymorphisms were identified by quantitative PCR.

57 Quantitative PCR, 16S rRNA gene metabarcoding and shotgu

58 ts by flow cytometry, ELISA, RNA sequencing, quantitative PCR, adoptive transfer to mice, and measure

59 everaged reverse transcription and real-time quantitative PCR along with key control experiments to q

60 Herein, using has deletion mutants, quantitative PCR analyses, and immunoblotting, we show t

61 site-directed mutagenesis, allelic exchange, quantitative PCR analyses, immunoblotting, and (13)C-hem

62 expression were measured by Western blot and quantitative PCR analyses, respectively, and in a subset

63 icroscopy, along with gene microarray and RT-quantitative PCR analyses, revealed that UNC45A localize

64 ion profiles were compared by microarray and quantitative PCR analyses.

65 smic and nuclear accumulation, and data from quantitative PCR analysis closely recapitulated the EdU

66 Quantitative PCR analysis confirmed SH3TC2 mRNA expressi

67 ofiles using multiple V regions validated by quantitative PCR analysis confirmed that distinct bacter

68 l evaluation in a blinded fashion as well as quantitative PCR analysis for chemokines and cytokines.

69 Moreover, RT-quantitative PCR analysis of actively translated mRNAs b

70 dual disease level was measured by real-time quantitative PCR analysis of immunoglobulin and T-cell r

71 Real-time quantitative PCR analysis revealed that 5azaD/TSA-expand

72 Finally, quantitative PCR analysis showed that expression of C. t

73 similar to Nesprin2-KD cells as assessed by quantitative PCR analysis.

74 assessed by histologic, flow cytometric, and quantitative PCR analysis.

75 t baseline and after 4 weeks and analyzed by quantitative PCR and 16S rRNA sequencing.

76 We used quantitative PCR and 16S rRNA-based sequencing to establ

77 nd its relevant matrix genes was measured by quantitative PCR and also analyzed in single-cell RNA-se

78 mtDNA damage was measured by using quantitative PCR and apoptosis via ELISA.

79 diarrheal controls for enteropathogens using quantitative PCR and calculated pathogen-specific attrib

80 We measured Nosema load with quantitative PCR and characterized microbiota with 16S r

81 Quantitative PCR and chromatin immunoprecipitation were

82 the number of CFHR3 and CFHR1 gene copies by quantitative PCR and collected clinical and biologic dat

83 Using quantitative PCR and DGGE profiling, we investigated fea

84 cytokine and chemokine levels by performing quantitative PCR and ELISA.

85 upregulated or downregulated using real-time quantitative PCR and found a strong correlation between

86 ytic bacterial communities was determined by quantitative PCR and high-throughput sequencing of 16S r

87 , diversity and composition were revealed by quantitative PCR and high-throughput sequencing.

88 Quantitative PCR and Illumina sequencing of the 16S rRNA

89 sion of Gprc5b in disease was analyzed using quantitative PCR and immunofluorescence, and by analyzin

90 Quantitative PCR and immunohistochemistry showed that HO

91 Senescence and fibrosis were measured by quantitative PCR and immunohistochemistry.

92 biliary hyperplasia and hepatic fibrosis by quantitative PCR and immunostaining of specific markers.

93 BDNF mRNA levels, assessed by quantitative PCR and in situ hybridization at 1, 4, 7, a

94 VL was determined using quantitative PCR and log10 transformed for normalization

95 RT-quantitative PCR and luciferase assays indicated that th

96 s titration results to reverse transcriptase quantitative PCR and measurement of fluorescein concentr

97 nchial soluble mediators were measured using quantitative PCR and MesoScale Discovery.

98 After confirmatory quantitative PCR and pathobiont-specific gene analyses,

99 ule primordia, chromatin immunoprecipitation-quantitative PCR and protoplast trans-activation assays

100 Using quantitative PCR and RNA flow cytometry, we found that c

101 Multiplex quantitative PCR and routine microbiologic culture were

102 pression and activity were assessed by using quantitative PCR and the telomere repeat amplification p

103 of differentiation markers was evaluated by quantitative PCR and Western blot.

104 n MGC and CGC and in human ovarian tissue by quantitative PCR and Western blot/immunohistochemistry,

105 dermal keratinocytes (n = 11) with real-time quantitative PCR and Western blotting revealed up-regula

106 We used quantitative PCRs and a telomere-sequence to single-copy

107 shmania donovani, spatial analyses at macro-(quantitative PCR) and micro-(confocal microscopy) scales

108 g immunoprecipitation and immunoblotting, RT-quantitative PCR, and ChIP assays, we show that SLTM int

109 work, using ChIP-sequencing, immunoblotting, quantitative PCR, and computational analyses across vari

110 etry, Toll-like receptor (TLR) expression by quantitative PCR, and cytokine secretion after stimulati

111 We validated the RNA-Seq data by quantitative PCR, and examined the expression pattern of

112 ith the use of micro-ELISAs, RNA sequencing, quantitative PCR, and flow cytometry, we found that ECs

113 liquid chromatography, reverse transcription-quantitative PCR, and genomic sequencing.

114 infection in vitro Using infectivity assays, quantitative PCR, and immunofluorescence assay approache

115 rotarod tests, followed by Western blotting, quantitative PCR, and immunohistochemistry to assess YY1

116 led-template shotgun metagenomic sequencing, quantitative PCR, and isolate whole-genome sequencing we

117 yzed by microarray and reverse transcription quantitative PCR, and linked with function by further pr

118 eic acid were observed in aborted fetuses by quantitative PCR, and PCV3 antigen was localized in hist

119 ase of 48.2- and 86.1-fold in mtDNA based on quantitative PCR, and proportion of subsequent short seq

120 ls segregating for different spot positions, quantitative PCR, and pyrosequencing, were used to confi

121 luding fluorescence and Electron microscopy, quantitative PCR, and RNAi-mediated depletion, we report

122 Our in situ hybridization, real-time quantitative PCR, and stage-specific transcriptomic anal

123 for a concurrent Orientia or Rickettsia spp. quantitative PCR, and the use of antibiotics by the pati

124 One-step growth, reverse transcription-quantitative PCR, and Western blotting assessments showe

125 of TGF-beta signaling pathways by real-time quantitative PCR array, supported the hypothesis that in

126 e EoE diagnostic panel, a 94-gene expression quantitative PCR array.

127 ted PCR targeting the cytochrome b gene) and quantitative PCR as reference standards.

128 In this analysis, we employed quantitative PCR as well as multiple sequencing methodol

129 erum tryptase determination, allele-specific quantitative PCR (ASqPCR) for the KIT D816V mutation, an

130 Through use of a quantitative PCR assay for HHpgV-1, infection was also d

131 Furthermore, chromatin immunoprecipitation-quantitative PCR assay showed enrichment of p63 on Sun1,

132 s used to evaluate viral inactivation, and a quantitative PCR assay was used to quantify DNA damage.

133 , were tested as unknowns using 10 different quantitative PCR assays at 5 different laboratories.

134 icipating in proficiency testing surveys for quantitative PCR assays of cytomegalovirus (CMV), Epstei

135 We used quantitative PCR assays to determine if presence or conc

136 ation, prespacer protection, sequencing, and quantitative PCR assays, we show here that the bacterial

137 assessed using microscopy and ultrasensitive quantitative PCR at intervals of 28 days from 12 to 20 w

138 We developed a quantitative PCR-based lymph node infiltration assay to

139 (ChIP-seq) and chromatin immunoprecipitation quantitative PCR (ChIP-qPCR) indicate that in LCLs inhib

140 ted cytokines was also detected by real-time quantitative PCR comparing lesional with perilesional or

141 s in StMSI1-OE Chromatin immunoprecipitation-quantitative PCR confirmed H3K27me3-mediated suppression

142 oms (POS) from 65 individuals with real-time quantitative PCR-confirmed SARS-CoV-2 infection, we show

143 e causing infection as there are no accepted quantitative PCR cutoffs for diagnosing respiratory vira

144 ChIP-quantitative PCR demonstrated that H3K9me3 is erased fro

145 Although culture and quantitative PCR detect active infection, currently used

146 In contrast, quantitative PCR detected the presence of the gene for i

147 using conventional reverse transcription and quantitative PCR detection.

148 ric oxide synthase expression (determined by quantitative PCR) differentiated AD and psoriasis with 1

149 were subjected to mRNA extraction, real-time quantitative PCR, enzyme-linked immunosorbent assay, and

150 Samples were tested by multiplex semi-quantitative PCR for 12 viruses.

151 Quantitative PCR for BCR-ABL1 yielded a value of 29.6% o

152 rom BLT1(+/+) and/or BLT1(-/-) mice and used quantitative PCR for gene expression analysis in termina

153 (PCR) for detecting messenger RNA (mRNA) and quantitative PCR for HBoV1 genome load count, we studied

154 Newer methods have proliferated and include quantitative PCR for organism load, AmpliSens PCR, PCR f

155 ls of miRs-1204-1208 were investigated using quantitative PCR for prostate cancer cell lines and prim

156 vitro differentiation of primary CD34 cells, quantitative PCR for unfolded protein response (UPR) gen

157 Quantitative PCR gene expression studies of glycosyltran

158 Quantitative PCR highlighted significant changes in expr

159 cyte-derived macrophages was investigated by quantitative PCR, immunoblotting and flow cytometry.

160 d in different malt and feed varieties using quantitative PCR, immunoblotting, and enzyme-linked immu

161 e model and primary cell cultures along with quantitative PCR, immunoblotting, and immunohistochemist

162 Using murine knock-in technology and quantitative PCR, immunoblotting, and immunoprecipitatio

163 Using immunohistochemistry, real-time quantitative PCR, immunoblotting, ELISA, siRNA-mediated

164 s and approaches, including cell biology, RT-quantitative PCR, immunoblotting, immunofluorescence, fl

165 Using real-time quantitative PCR, immunofluorescence, Western blotting,

166 The HP CNV was typed using quantitative PCR in 1299 aSAH survivors in the Genetics

167 The levels of serum miRNAs were performed by quantitative PCR in 138 asthmatics and 39 healthy subjec

168 next-generation sequencing and confirmed by quantitative PCR in 29 asthmatics and 10 healthy individ

169 d Cacna1c expression levels were examined by quantitative PCR in fluorescence-activated cell sorting.

170 ngesting modified LDL; this was validated by quantitative PCR in human and murine macrophages.

171 -34 mRNA levels were quantified by real-time quantitative PCR in human synovial fibroblasts and murin

172 immunoassay and Campylobacter jejuni/coli by quantitative PCR in stool samples.

173 We measured TL by quantitative PCR in white Swiss HIV Cohort Study partici

174 Validation by quantitative PCR indicated a high reliability of the RNA

175 ng microarray, immunoblotting, and real-time quantitative PCR indicated that PIERCE1 negatively regul

176 NA polymerase (RNAP) activity using a common quantitative PCR instrument for fluorescence detection.

177 ession was examined by reverse-transcriptase quantitative PCR, intracellular flow cytometry, and ELIS

178 Quantitative PCR is a diagnostic mainstay of clinical vi

179 Real-time quantitative PCR may be useful in differentiating asympt

180 Real-time quantitative PCR measured CSF2 and CSF2R mRNA levels.

181 s process, we correlated these findings with quantitative PCR measurements and protein analyses of gl

182 Quantitative PCR measurements of PMP22 mRNA in dermal ne

183 either directly, using reverse transcription-quantitative PCR, microarrays, and flow cytometry, or in

184 alysis, identification of cargo microRNA via quantitative PCR, microRNA target validation by overexpr

185 med methylation-sensitive restriction enzyme-quantitative PCR (MSRE-qPCR) and observed that multiple

186 ) and validated by reverse transcription and quantitative PCR (n = 40).

187 Flow cytometry, quantitative PCR, next-generation sequencing, and specif

188 AZM-treated patients) had sputum stored for quantitative PCR of 6GS markers at baseline and after 48

189 Quantitative PCR of exon versus intron sequences confirm

190 The titer of the bioamendments based on quantitative PCR of functional marker genes decreased bu

191 e chain reaction analysis and taxon-specific quantitative PCR of the 16S ribosomal RNA gene.

192 -5, and IL-10 mRNA was measured by real-time quantitative PCR on tissue homogenates of patients with

193 documented PG-G hydrolases in neutrophils by quantitative PCR, only ABHD12 and ABHD16A were detected

194 bsence of certain antibiotics with real-time quantitative PCR or digital PCR to determine antimicrobi

195 NA, and cytokines were assessed by real-time quantitative PCR or ELISA tests.

196 han did endoscopic brushes or biopsies using quantitative PCR (p<0.0001).

197 op-mediated isothermal amplification; or (4) quantitative PCR positive with >5 parasites/uL.

198 Using quantitative PCR (q-PCR), immunostaining and patch clamp

199 Quantitative PCR (qPCR) allows the precise measurement o

200 Nucleic acid based techniques, such as quantitative PCR (qPCR) and next generation sequencing (

201 scribe a relatively high-throughput combined quantitative PCR (qPCR) and next-generation sequencing m

202 Here, ddPCR was compared to quantitative PCR (qPCR) and pyrosequencing.

203 and caspase assays; and gene expression with quantitative PCR (qPCR) and RNA sequencing on lung epith

204 Here we describe a novel real-time quantitative PCR (qPCR) approach based on the SYBR green

205 ion was compared to that with a conventional quantitative PCR (qPCR) assay and blood culture.

206 of the Xpert Carba-R test, a rapid real-time quantitative PCR (qPCR) assay that detects five families

207 We designed a multiplex real-time quantitative PCR (qPCR) assay to detect SFGR, TGR, O. ts

208 ement, RLPM, upon which a specific real-time quantitative PCR (qPCR) assay was developed.

209 rkeri or "Candidatus Rickettsia andeanae," a quantitative PCR (qPCR) assay was employed to quantify r

210 wo molecular approaches: (i) a comprehensive quantitative PCR (qPCR) assay, including testing for aer

211 Quantitative PCR (qPCR) assays are the gold standard for

212 performance of the Pan-LASV RDT to available quantitative PCR (qPCR) assays during the 2018 LF outbre

213 triplex TaqMan MGB probe-based fluorescence quantitative PCR (qPCR) assays to perform both qualitati

214 In this study, 34 probe-based quantitative PCR (qPCR) assays were designed to target a

215 Through a series of immunoblotting and quantitative PCR (qPCR) experiments, we show that Msp do

216 Using quantitative PCR (qPCR) for common respiratory viruses a

217 mong diarrheal samples positive by nanoliter quantitative PCR (qPCR) for V. cholerae (n = 78/849), th

218 Quantitative PCR (qPCR) has become the gold standard tec

219 Real-time quantitative PCR (qPCR) is one of the most powerful tech

220 showed a total percent agreement of mNGS and quantitative PCR (qPCR) of 89.2% (306/343), with a kappa

221 ory protein database with RNA-sequencing and quantitative PCR (qPCR) results in eWAT, the mRNA levels

222 s were analyzed by using a manual, nonnested quantitative PCR (qPCR) targeting IS6110 Two swab brands

223 However, practices learned from quantitative PCR (qPCR) that promote assay robustness an

224 We performed real-time quantitative PCR (qPCR) to determine the abundance of Br

225 ion-sensitive restriction enzymes (MSRE) and quantitative PCR (qPCR) to determine the methylation sta

226 for 20 min and the use of PerfeCTa multiplex quantitative PCR (qPCR) ToughMix.

227 In experiment 1, quantitative PCR (qPCR) was performed at several stages

228 Rapid diagnostic test and quantitative PCR (qPCR) were used for the detection of i

229 In this study, we compared the results of B1 quantitative PCR (qPCR) with those of two different Rep

230 men were tested by microscopy (micromethod), quantitative PCR (qPCR), and immunoglobulin (Ig)M trypom

231 Using quantitative PCR (qPCR), droplet digital PCR, and fluore

232 Its advantages over quantitative PCR (qPCR), including absolute quantificati

233 Unlike quantitative PCR (qPCR), the developed HDA-strip assay o

234 Using quantitative PCR (qPCR), the efficiency of each selectio

235 entified 45 of 48 that were PCV3 positive by quantitative PCR (qPCR), with 60% of a subset also testi

236 further studied through field surveys using quantitative PCR (qPCR), with the conclusion that it was

237 ent, BV Blue, and Affirm VPIII, as well as a quantitative PCR (qPCR)-based test under initial evaluat

238 and NGU, and to select bacteria for targeted quantitative PCR (qPCR).

239 well as the density of bacteria in stool by quantitative PCR (qPCR).

240 ochrome P450 17A1 [CYP17A1]), as measured by quantitative PCR (qPCR).

241 nd HIV RNA (CA-DNA, CA-RNA) were measured by quantitative PCR (qPCR).

242 a walk-away workflow identical to real-time quantitative PCR (qPCR).

243 nely applied after culture (spoligotyping or quantitative PCR [qPCR]) in each laboratory; liquid (MGI

244 ) than in the one without T (porA) Real-time quantitative PCR (qRT-PCR) analysis of the porA mRNA and

245 Quantitative PCR (qRT-PCR) and Western blotting confirme

246 fied by microarray and reverse transcription-quantitative PCR (qRT-PCR) in the screening cohort.

247 Using SnoRNASeq and real-time quantitative PCR (qRT-PCR) we demonstrate snoRNA express

248 ole-genome sequencing, reverse transcription-quantitative PCR (qRT-PCR), and carbohydrate utilization

249 vels were assessed via reverse transcriptase quantitative PCR (qRT-PCR).

250 Moreover, tandem affinity and RT-quantitative PCR results revealed that JUND mRNA is a co

251 e3 in vivo and chromatin immunoprecipitation-quantitative PCR results showed binding occurs preferent

252 Quantitative PCR revealed higher Gabrd transcript levels

253 roxia-exposed samples were made by real-time quantitative PCR, RNA in situ hybridization, quantitativ

254 Reverse-transcriptase-quantitative PCR (RT-Q-PCR) and RT-PCR amplicon sequenci

255 al abundances were validated using real-time quantitative PCR (RT-qPCR) and Northern blotting.

256 ares the accuracies of reverse transcription-quantitative PCR (RT-qPCR) and reverse transcription-dig

257 A real-time reverse transcription-quantitative PCR (RT-qPCR) assay utilizing Pleiades prob

258 Reverse transcriptase quantitative PCR (RT-qPCR) confirmed the presence of vir

259 y RNA-seq or reverse transcription-real-time quantitative PCR (RT-qPCR) in whole-blood samples from s

260 patients with NASH, as assessed by real time quantitative PCR (RT-qPCR) or western blots.

261 -16S rRNA, and rpoB by reverse transcriptase quantitative PCR (RT-qPCR) showed minimal loss in estima

262 by immunofluorescence, reverse transcriptase quantitative PCR (RT-qPCR), and NGS.

263 mation, mainly through reverse transcription-quantitative PCR (RT-qPCR), is a common approach employe

264 conventional viral RNA reverse transcription-quantitative PCR (RT-qPCR)-based assays and from outgrow

265 for genotype A measles virus (MeV) (MeVA RT-quantitative PCR [RT-qPCR]) that can identify measles va

266 By combining targeted quantitative PCR screens with bioinformatic analysis and

267 Quantitative PCR showed that the mRNA levels of chemokin

268 Allele-specific quantitative PCR showed that the vast majority of ILC2s

269 We demonstrate that sADPL coupled to quantitative PCR signal detection enables multiplexed "w

270 Quantitative PCR study indicates that 5-aza may function

271 at swabs were positive for SARS-CoV-2 RNA by quantitative PCR, suggesting community transmission of S

272 Pneumococcal DNA was detected with quantitative-PCRs targeting piaB and lytA genes in raw a

273 airways, RNAscope in situ hybridization and quantitative PCR to assess CFTR mRNA expression in the l

274 at quarterly intervals using ultrasensitive quantitative PCR to detect Plasmodium infections.

275 We used quantitative PCR to generate a composite metric of type

276 3 years or more, with three or more BCR-ABL quantitative PCR transcript measurements (BCR-ABL to ABL

277 onducted on blood samples, using high-volume quantitative PCR (uPCR).

278 that of ultrasensitive reverse transcription-quantitative PCR (uRT-qPCR) using nucleic acid extracts

279 ollowed by differential expression analysis, quantitative PCR validation and detailed investigation o

280 cell RNA-sequencing data sets in tandem with quantitative PCR validation in both murine and human isl

281 Targeted RT-quantitative PCR verified the altered expression of sele

282 Real Time-quantitative PCR was performed for SERPINA3 transcript,

283 n was measured by using ELISA, and real-time quantitative PCR was performed to detect PGE2 receptor e

284 Quantitative PCR was used to compare bacterial load and

285 Flow cytometry and quantitative PCR was used to measure type 2 cytokines.

286 Quantitative-PCR was used to assess DNA concentrations o

287 Then, using real-time quantitative PCR we found that PROX1 displays a strong a

288 Using quantitative PCR, we determined that two vascular pathog

289 orescence in situ hybridization analysis and quantitative PCR, we found that NA inhibited gene expres

290 Using genomic data and quantitative PCR, we show that SCN4A is on the Z chromos

291 Upon validation with quantitative PCR, we studied the association of the top

292 ption at low dNTP concentrations followed by quantitative-PCR, we found that the same adenosine resid

293 tance genes.Methods: 16S rRNA sequencing and quantitative PCR were performed to assess the effect of

294 situ activity assays, Western blotting, and quantitative PCR were used to investigate function and e

295 e threshold (C(T) ) values, determined using quantitative PCR, were higher for GPP-negative/culture-p

296 from our own molecular genetic experiments (quantitative PCR, Western blot, EMSA) or genome-wide ass

297 iratory syndrome 2 (SARS-CoV-2) infection by quantitative PCR with reverse transcription (RT-qPCR) ty

298 surgery, SHERLOCK was in 100% agreement with quantitative PCR with reverse transcription.

299 5p replicates in an independent cohort using quantitative PCR, with concomitant upregulation of four

300 Quantitative PCR yielded no Escherichia coli and few ins