ライフサイエンスコーパス: quantitative real-time PCR

1 nce it was first described in the mid-1990s, quantitative real time PCR (Q-PCR) has been widely used

2 A quantitative real-time PCR (qRT-PCR) assay with single-c

3 A quantitative real-time PCR (RT-PCR) method, employing no

4 Additionally, a quantitative real-time PCR (qPCR) was performed to estim

5 Additionally, a quantitative real-time PCR approach was proposed, allowi

6 Employing a quantitative real-time PCR assay, we determined the repl

7 In addition, by using a quantitative real-time PCR-based viral array, we are the

8 e gene expression analysis and would achieve quantitative real-time PCR replication pre-ITx (another

9 In addition, quantitative real-time PCR revealed that intracellular a

10 Additionally, quantitative real-time PCR studies showed that the trans

11 We analyzed quantitative real-time PCR (qPCR) assays on DNA extracte

12 a chromosome conformation capture assay and quantitative real time PCR, we demonstrate that a chroma

13 ometry, ELISA, RNA in situ hybridization and quantitative real time PCR.

14 chemical in situ hybridization histology and quantitative real time-PCR studies.

15 ods of 4 days during the summer of 2012, and quantitative real-time PCR (qPCR) was used to enumerate

16 red and subjected to microarray analysis and quantitative real-time PCR (qRT-PCR).

17 Here, microarray analysis and quantitative real-time PCR showed that miR-365 was robus

18 Cell viability assays and quantitative real-time PCR showed that the peptide inter

19 Biochemical assays and quantitative real-time PCR were used to measure triglyce

20 amic areas were analysed by Western blot and quantitative real-time PCR (qRT-PCR) for expression of a

21 Using western blot and quantitative real-time PCR analysis, our results indicat

22 d to quantify the biomass in flow cells, and quantitative real-time PCR with species-specific primers

23 ccolithovirus strains, by flow cytometry and quantitative real-time PCR allowed tentative links to th

24 ction were examined using flow cytometry and quantitative real-time PCR.

25 gated using plate counts, flow cytometry and quantitative real-time PCR.

26 use of optical density, flow cytometry, and quantitative real-time PCR (qPCR).

27 cates, using droplet digital PCR (ddPCR) and quantitative real-time PCR.

28 fluorescence, enzymatic assays (ELISA), and quantitative real-time PCR.

29 s as shown by both in situ hybridization and quantitative real-time PCR.

30 orbent assay, RNA in situ hybridization, and quantitative real-time PCR.

31 ed and analyzed for C5L2 and C5aR by IHC and quantitative real-time PCR.

32 rmed in cultured cells by immunoblotting and quantitative real-time PCR and in mouse kidneys by immun

33 on assays as well as with immunoblotting and quantitative real-time PCR for the expression of stem ce

34 17 signature by using immunofluorescence and quantitative real-time PCR.

35 3) was monitored by immunohistochemistry and quantitative real-time PCR (qRT-PCR).

36 ors was assessed by immunohistochemistry and quantitative real-time PCR in isolated islets and adenom

37 We used immunohistochemistry and quantitative real-time PCR to determine IL-31 expression

38 Using immunohistochemistry and quantitative real-time PCR, we assessed biopsy specimens

39 Using in situ hybridization (ISH) and quantitative real-time PCR (qPCR), we assess the mRNA di

40 Using whole-genome microarray and quantitative real-time PCR analysis of endobronchial bio

41 ssion was measured by using a microarray and quantitative real-time PCR.

42 in a subset of GABAergic alBST neurons, and quantitative real-time PCR confirmed that GLP1R-KD rats

43 ive donor samples by in-house nested PCR and quantitative real-time PCR assays, respectively.

44 nfiltrate composition, by in situ RT-PCR and quantitative real-time PCR of laser microdissected islet

45 were validated by ChIP-quantitative PCR and quantitative real-time PCR.

46 ultiplex polymerase chain reaction (PCR) and quantitative real-time PCR.

47 based PCR and reverse transcriptase PCR, and quantitative real-time PCR (qPCR).

48 Using mature miRNA profiling and quantitative real-time PCR (qRT-PCR) in the orbitofronta

49 red their mature, phasic firing property and quantitative real-time PCR confirmed a coincident increa

50 measured by solid phase radioimmunoassay and quantitative real-time PCR.

51 Microarray analysis revealed and quantitative real-time PCR (qRT-PCR) confirmed that grow

52 E, and phnAB occurs in these revertants, and quantitative real-time PCR experiments suggested that th

53 RNA sequencing and quantitative real-time PCR analyses revealed that, altho

54 RNA sequencing and quantitative real-time PCR analysis were used to assess

55 High-throughput sequencing and quantitative real-time PCR showed a significant composit

56 Here, we used next-generation sequencing and quantitative real-time PCR to determine the impact of dr

57 is suppressed were assayed by microarray and quantitative real-time-PCR analyses.

58 Microarray and quantitative real-time-PCR analysis of gonads showed ele

59 Furthermore, the LC-MS/MS and quantitative real-time-PCR analysis followed by inhibito

60 We also apply quantitative real-time PCR to several highly ranked non-

61 rgets were assessed using luciferase assays, quantitative real-time PCR and western blots in vitro an

62 ivity were assessed using luciferase assays, quantitative real-time PCR, western blots, scratch assay

63 Two quantitative assays, quantitative real-time PCR (qRT-PCR) and droplet digital

64 vivo confocal microscopy (IVCM) assessment, quantitative real-time PCR gene expression, C. trachomat

65 now developed a rapid peripheral blood-based quantitative real-time PCR assay that enables accurate p

66 ction of miRNAs including northern blotting, quantitative real time PCR (qRT-PCR) and microarray tech

67 Both quantitative real-time PCR (qRT-PCR) and Western blottin

68 By quantitative real time PCR, the level of kindlin-3 in en

69 duce and their expression levels analyzed by quantitative real time PCR (qRT-PCR) at different time p

70 Fibrotic response was assessed by quantitative real time PCR (qRT-PCR) of fibrotic marker

71 at mRNA and protein levels was confirmed by quantitative real time PCR (qRT-PCR) and immunocytochemi

72 some genes were selected to be evaluated by quantitative real time PCR (RT-qPCR), and also functiona

73 cle actin (alphaSMA) and Smad7 expression by quantitative real time PCR.

74 These miRs were validated by quantitative real time-PCR (qRT-PCR) in 43 patients with

75 By quantitative real-time PCR, we determined the mRNA copy

76 investigated in three sfr6 mutant alleles by quantitative real-time PCR and susceptibility to UV-C ir

77 alongside with measuring the virus amount by quantitative real-time PCR.

78 MiR-125b expression was analyzed by quantitative real-time PCR and in situ hybridization in

79 , four were selected and further analyzed by quantitative real-time PCR at the transcriptome level to

80 a-submerged polystyrene pegs was analyzed by quantitative real-time PCR using species-specific primer

81 monitored by immunohistofluorescence and by quantitative real-time PCR measurement of Foxp3 mRNA.

82 cence assay to monitor NS1 expression and by quantitative real-time PCR.

83 enotyping accuracy of 99%, as ascertained by quantitative real-time PCR.

84 ession of Stat5 target genes were assayed by quantitative real-time PCR assay.

85 Gene expression was assessed by quantitative real-time PCR (QPCR) in one center.

86 xpression, and localization were assessed by quantitative real-time PCR, Western blot analysis, and i

87 PEX material and TGF-beta1 were assessed by quantitative real-time PCR, Western blotting, immunohist

88 IFN-gamma, PD-1, and PD-L1 were assessed by quantitative real-time PCR.

89 y IHC, and immune mediators were assessed by quantitative real-time PCR.

90 tary zinc depletion were further assessed by quantitative real-time PCR.

91 sured in lysates from bronchial brushings by quantitative real-time PCR and Western blot.

92 We determined the KIT D816V allele burden by quantitative real-time PCR in bone marrow and peripheral

93 for the expression of relevant chemokines by quantitative real-time PCR and immunohistochemistry.

94 rimary murine NK cells, with confirmation by quantitative real-time PCR (qRT-PCR) and microarrays.

95 in the venom gland and this was confirmed by quantitative real-time PCR (qPCR) analysis, suggesting t

96 fear conditioning, miR-182, was confirmed by quantitative real-time PCR.

97 Gene expression was confirmed by quantitative real-time PCR.

98 icroRNA-21 (miR-21) was further confirmed by quantitative real-time PCR.

99 ly selected colorectal cancer-derived CTC by quantitative real-time PCR (qRT-PCR) to investigate tran

100 isk (LR) forms of PCa, was not detectable by quantitative real-time PCR in samples from healthy volun

101 se of differentiating cells were detected by quantitative real-time PCR.

102 eukin-22 (IL-22), and IL-17 were detected by quantitative real-time PCR.

103 CFU determination by quantitative real-time PCR (qPCR), however, is becoming

104 As determined by quantitative real-time PCR analysis, we found that level

105 smids is 23 in M. smegmatis as determined by quantitative real-time PCR and accounts for the previous

106 Renal Klotho expression was determined by quantitative real-time PCR and immunoblot analysis.

107 Gene expression was determined by quantitative real-time PCR and protein expression by Wes

108 and protein levels of CLN3 as determined by quantitative real-time PCR and Western blotting.

109 MP9 expression and activity as determined by quantitative real-time PCR and zymography.

110 MC markers were determined by quantitative real-time PCR for chymase and c-kit.

111 a/b/c, miR-155, and let-7a was determined by quantitative real-time PCR in formalin-fixed paraffin-em

112 ulatory and fibrosis genes was determined by quantitative real-time PCR in livers and hearts of 52-we

113 periodontopathic bacteria were determined by quantitative real-time PCR.

114 In addition, we established by quantitative real-time PCR (qRT-PCR) that both fresh col

115 elevated more than 100-fold as estimated by quantitative real-time PCR in most MPNST cell lines.

116 Adiponectin mRNA levels were evaluated by quantitative real-time PCR in the visceral adipose tissu

117 d gene expression profiles were evaluated by quantitative real-time PCR.

118 Changes in gene expression were evaluated by quantitative real-time PCR.

119 esenchymal marker expression was examined by quantitative real-time PCR, Western blotting, and immuno

120 We have identified, by quantitative real-time PCR, hundreds of miRNAs that are

121 pendent cohort of patient-matched lesions by quantitative real-time PCR.

122 s of cuticle-associated gene mRNA levels, by quantitative real-time PCR, indicated a relationship bet

123 ns with different median strain lifespans by quantitative real-time PCR.

124 Expression of 41 unigenes measured by quantitative real-time PCR (qRT-PCR) showed consistent r

125 the symptomatic phase of ECM, as measured by quantitative real-time PCR analysis.

126 Beta-cell gene profiles were measured by quantitative real-time PCR and the effects on intracellu

127 and protein by more than 50% as measured by quantitative real-time PCR and Western blot analysis.

128 n of miRNA and target genes were measured by quantitative real-time PCR and/or Western blotting.

129 Measured by quantitative real-time PCR, capn1 transcript levels were

130 ssion of gluconeogenic genes was measured by quantitative real-time PCR.

131 We measured levels of microRNAs and mRNAs by quantitative real-time PCR analysis of RNA extracted fro

132 erfusion-induced microglial ISG responses by quantitative real-time PCR and demonstrated that both we

133 normal and malignant endometrial samples by quantitative real-time PCR and Northern blot analysis re

134 n lesional, nonlesional, and healthy skin by quantitative real-time PCR and immunohistochemistry, and

135 for bisulfide detection in bulk solution by quantitative real-time PCR, which achieved a linear work

136 ond larger cohort of ESCC tumor specimens by quantitative real-time PCR and immunohistochemical analy

137 localization of Populus PHYs was studied by quantitative real-time PCR (qRT-PCR) and protoplast tran

138 oropharyngeal swab specimens were tested by quantitative real-time PCR (qPCR) for feline herpesvirus

139 fied IL-10-inhibited genes also validated by quantitative real-time PCR (qRT-PCR) were Toll-like rece

140 I were identified in silico and validated by quantitative real-time PCR (qRT-PCR), Western blot analy

141 NA microarrays and were further validated by quantitative real-time PCR analysis.

142 RNA-Seq data was validated by quantitative real-time PCR of selected differentially ex

143 expression analysis, which was validated by quantitative real-time PCR on a subset of genes, showed

144 riority candidates for further validation by quantitative real-time PCR analysis.

145 We validated these results by quantitative real-time-PCR using NBUVB-treated vitiligo

146 Using a model of persistent pain, we show by quantitative real-time-PCR, florescence in situ hybridiz

147 nents of a dysbindin interaction network by, quantitative real time PCR and quantitative immunohistoc

148 Single-cell quantitative real-time PCR (qRT-PCR) combined with high-

149 sing an array of approaches, including ChIP, quantitative real-time PCR, and cell migration assays, w

150 They conducted quantitative real-time PCR on genomic DNA isolated from

151 filtrated pancreatic islets and confirmatory quantitative real time PCR and protein analyses identifi

152 il during plant growth using plate counting, quantitative real-time PCR, and metagenomics analysis.

153 Here, using cytokine, quantitative real-time PCR, immunoprecipitation, and ChI

154 We developed quantitative real-time PCR (qPCR) assays to facilitate a

155 Here we employed quantitative real-time PCR (qPCR) assays for polyphospha

156 We evaluated quantitative real-time PCR to establish the diagnosis of

157 LncRNA MALAT1 expression (quantitative real-time PCR, immunofluorescence, and RNA

158 etry), cytokine mRNA and protein expression (quantitative real-time PCR and immunofluorescence), micr

159 m virgin and age-matched postpartum females, quantitative real-time PCR and Western blotting were car

160 Consistent with these findings, quantitative real-time PCR (qRT-PCR) and enzyme-linked i

161 We sought to identify reference genes for quantitative real-time PCR (qPCR) and validate RNAi resp

162 ntroduce a plasmonic photothermal method for quantitative real-time PCR, using gold bipyramids and li

163 se (GAPDH) and 18S ribosomal RNA (rRNA), for quantitative real-time PCR.

164 In this study, results from quantitative real-time PCR (qRT-PCR) assays are used as

165 Next-generation sequencing, 16 S rRNA gene quantitative real-time PCR, and aerobic culturing were a

166 Single-gene quantitative real-time PCR, immunoblot, and immunofluore

167 gmentosum (XP) A and other DNA repair genes (quantitative real-time PCR analysis) and resulted in an

168 Here, quantitative real-time PCR analyses revealed that brains

169 ells and BAL fluid were evaluated for HPGDS (quantitative real-time PCR/immunohistochemistry [IHC]) a

170 t were examined using in situ hybridization, quantitative real-time PCR, Western blot analysis and fi

171 lonization by (i) classical culture and (ii) quantitative real-time PCR (qPCR) targetinglytAin patien

172 Immunohistochemistry, immunofluorescence, quantitative real-time PCR, in vivo pharmacology, Wester

173 ubjects (n = 71) using immunohistochemistry, quantitative real-time PCR, and Singulex in a cross-sect

174 t of patients, lymphocyte immunophenotyping, quantitative real-time PCR from nasopharyngeal swabs, an

175 We used immunostaining, quantitative real-time PCR, Western blots, and lung canc

176 ere, using an array of techniques, including quantitative real-time PCR, immunostaining, reporter gen

177 onal antibodies coupled with mRNA isolation, quantitative real-time PCR, and standard PCR analyses co

178 ergillus-specific lateral-flow device (LFD), quantitative real-time PCR (qPCR), and the galactomannan

179 Padlock probe-mediated quantitative real time PCR (PLP-qRT-PCR) was adapted to

180 pithelium were determined by DNA microarray, quantitative real-time PCR, and immunohistochemistry bas

181 variety of approaches, including microarray, quantitative real-time PCR, Western blotting, chromatin

182 T cells was measured by means of microarray, quantitative real-time PCR, or both.

183 , such as bisulfite sequencing, microarrays, quantitative real-time PCR, colorimetry, Raman spectrosc

184 robial peptides was measured using multiplex quantitative real time PCR.

185 tudy for the first time explored a multiplex quantitative real-time PCR (qPCR) technique combined wit

186 escribe development of an absolute multiplex quantitative real-time PCR for detection of Plasmodium s

187 need, we developed both single and multiplex quantitative real-time PCR (qPCR) assays that can accura

188 After adaptation to multiplex quantitative real-time PCR (qRT-PCR), the signature was

189 ion were assessed by comparative analysis of quantitative real-time PCR (qrtPCR) by the delta-delta C

190 The application of quantitative real-time PCR (qPCR) technologies for the r

191 cell-SELEX, state-of-the-art applications of quantitative real-time PCR (qPCR), next-generation seque

192 We present an assay based on quantitative real-time PCR which estimates the relative

193 RT-PCR, quantitative real-time PCR and Western blotting were emp

194 Barr virus (EBV), were studied by performing quantitative real-time PCR analysis of blood samples obt

195 n of the genes that code identified protein, quantitative real time PCR (qRT-PCR) was performed.

196 K, which encodes an uncharacterized protein; quantitative real-time PCR (qRT-PCR) confirmed consisten

197 Relative quantitative real-time PCR (qRT-PCR) was used to determi

198 Here we show via relative quantitative real-time PCR and microarray analysis an in

199 d and 12 other FBLN5-regulated genes by semi-quantitative real time PCR.

200 ion in Golden Syrian hamsters by a sensitive quantitative real-time PCR (TaqMan) with lipl32 as the t

201 P was compared to that of a highly sensitive quantitative real-time PCR (qPCR) assay.

202 creening and treatment with highly sensitive quantitative real-time PCR, or MDA with blood-stage trea

203 ence-activated cell sorting, RNA sequencing, quantitative real-time PCR, Western blotting, small inte

204 of HCL, we developed a BRAFV600Emut-specific quantitative real-time PCR assay and validated it in 117

205 More specifically, quantitative real time PCR (qPCR) achieves a high degree

206 associated with using the analogue standard, quantitative real-time PCR (qPCR).

207 resveratrol, TNF-alpha, and staurosporine), quantitative real-time PCR and clustering analysis, we s

208 Subsequent quantitative real-time PCR (qRT-PCR) and Northern blotti

209 r cholesterol-coated surfaces and subsequent quantitative real-time PCR (qRT-PCR) revealed that type

210 RNAs were selected and validated by targeted quantitative real-time PCR.

211 The quantitative real time PCR analysis was based on a suppr

212 These results were also confirmed by the quantitative real-time PCR (qPCR), droplet digital PCR (

213 onated genes expression was assessed through quantitative real time PCR.

214 panel of human tissues was analysed through quantitative real-time PCR methods, to quantify the rela

215 specific transcripts were identified through quantitative real-time PCR.

216 Using a high-throughput quantitative real-time PCR platform, we performed miRNA

217 re length was measured using high-throughput quantitative real-time PCR.

218 extraction method for reverse transcriptase quantitative real-time PCR (RT-qPCR)-based detection app

219 throughput, stem-loop, reverse transcriptase quantitative real-time PCR miRNA expression profiling (s

220 situ hybridization and reverse transcriptase quantitative real-time PCR.

221 ession was assessed by reverse transcription quantitative real time PCR and function by Hoechst 33342

222 s-specific primers for reverse transcription quantitative real-time PCR (RT-qPCR) were designed by id

223 6 mRNA, as shown using reverse-transcription quantitative real-time PCR and luciferase reporter activ

224 Virus detection (reverse transcription-quantitative real-time PCR [RT-qPCR]) and genotyping (en

225 experiments, including in vitro translation, quantitative real-time PCR, Northern blot, ribonuclease

226 of over 1,700 publications whose authors use quantitative real-time PCR (qPCR) reveal a lack of trans

227 We also used quantitative real-time PCR to analyse a panel of cDNAs d

228 We used quantitative real-time PCR (qPCR) to test for 32 enterop

229 We used quantitative real-time PCR and IHC in human ICM and a ra

230 We used quantitative real-time PCR to analyze expression of neur

231 f the MCM family to GBM progression, we used quantitative real-time PCR to analyze the gene expressio

232 We used quantitative real-time PCR to determine DEFA1A3 DNA copy

233 vement in human cortical development we used quantitative real-time PCR to examine the expression tra

234 In this study, we used quantitative real-time-PCR to define miRNA expression pa

235 Using quantitative real time PCR and chromatin Immuno-Precipit

236 Using quantitative real time PCR, we have shown higher express

237 n and cellular responses were measured using quantitative real time PCR and multiplex assay.

238 , n = 59) and expression was validated using quantitative real time PCR (n = 21) and western blotting

239 Gene expression analysis using quantitative real time-PCR, GGPPS promoter-GUS (beta-glu

240 Using quantitative real-time PCR (q real-time PCR), YPEL5/PPP1

241 Using quantitative real-time PCR (qRT-PCR) we have measured th

242 Using quantitative real-time PCR (qRT-PCR), it showed that the

243 Using quantitative real-time PCR analysis, we find that 13 for

244 Using quantitative real-time PCR and functional drug efflux st

245 Using quantitative real-time PCR, GSTP1 gene expression was as

246 Using quantitative real-time PCR, the expression of twenty-sev

247 Using quantitative real-time PCR, we found that transcript lev

248 Using quantitative real-time PCR, we investigated the intra-he

249 Using quantitative real-time PCR, we prospectively measured re

250 Using quantitative real-time PCR, we showed that the block to

251 ndent gene expression in P. aeruginosa using quantitative real-time PCR.

252 culture-independent molecular analysis using quantitative real-time PCR (qPCR).

253 genes were selected for validation by using quantitative real-time PCR (qPCR), all of which were suc

254 CCA was analyzed at 24 h or 21 d by using quantitative real-time PCR and immunohistochemistry.

255 measured in different human tissues by using quantitative real-time PCR and immunohistochemistry.

256 on of the TH17 response was studied by using quantitative real-time PCR, flow cytometry, and addition

257 Results were validated by using quantitative real-time PCR, protein expression, and func

258 s, and mRNA expression was assessed by using quantitative real-time PCR.

259 XMRV and monitored virus production by using quantitative real-time PCR.

260 on DC subsets by HDM was quantified by using quantitative real-time PCR.

261 cies under different stress conditions using quantitative real-time PCR (qPCR).

262 This was confirmed using quantitative real-time PCR (n = 4).

263 and 16 periodontally healthy controls using quantitative real-time PCR (qRT-PCR).

264 growth-arrested embryos (degenerates) using quantitative real-time PCR (qRT-PCR).

265 y and gene transcripts were determined using quantitative real-time PCR.

266 nfirmation of gene expression was done using quantitative real-time PCR.

267 ures; these were validated empirically using quantitative real-time PCR to measure gene expression.

268 N) messenger RNA (mRNA), was evaluated using quantitative real-time PCR.

269 Further evaluation using quantitative real-time PCR revealed that differentially

270 umber of the NADH dehydrogenase 1 gene using quantitative real-time PCR.

271 Enumeration of Dhc biomarker genes using quantitative real-time PCR (qPCR) in groundwater is a ke

272 s was done for a select group of genes using quantitative real-time PCR (qRT-PCR).

273 respiratory pathogens, including HRV, using quantitative real-time PCR assays.

274 y and diseased tissue was investigated using quantitative real-time PCR.

275 Validation of candidate markers using quantitative real-time PCR (qRT-PCR) showed that two mar

276 mtDNA copy number was measured using quantitative real-time PCR on PBC DNA samples from parti

277 alpha-Synuclein mRNA levels, measured using quantitative real-time PCR, did not differ significantly

278 MT expression was measured using quantitative real-time PCR.

279 TNF]-alpha(KO), or caspase 7(KO) mice) using quantitative real-time PCR, enzyme-linked immunosorbent

280 yed for their increased levels of mRNA using quantitative real-time PCR.

281 t women and confirmed this observation using quantitative real-time PCR and mass spectrometry proteom

282 We validated the results obtained using quantitative real-time PCR (qPCR).

283 els were also evaluated in 69 patients using quantitative real-time PCR.

284 syndrome (MDS) and 76 healthy persons using quantitative real-time PCR.

285 pression in human tissues was profiled using quantitative real-time PCR.

286 , total bacterial load was quantitated using quantitative real-time PCR.

287 Studies using quantitative real-time PCR revealed stage-specific chang

288 ved in predation process was validated using quantitative real-time PCR (qRT-PCR).

289 Functional validation using quantitative real-time PCR (qRT-PCR) indicated four prom

290 es (LN) and peripheral blood cells utilizing quantitative real-time PCR at different time points post

291 by miR-21 in human and mouse cells, whereas quantitative real-time PCR revealed little difference at

292 ssion events that are highly correlated with quantitative real time PCR measurement.

293 analysis, and suspect screening, along with quantitative real-time PCR and time-resolved amplicon Il

294 Compared with quantitative real-time PCR (qPCR), metagenomic sequencin

295 This finding was corroborated with quantitative real-time PCR assays and protein immunoblot

296 g chromatin immunoprecipitation coupled with quantitative real-time PCR, we identified five putative

297 ing AKI was confirmed at the mRNA level with quantitative real-time PCR and at the protein level with

298 eleted mtDNA in each donor was measured with quantitative real-time PCR (qPCR).

299 he KCNQ2 and KCNQ3 subunits as measured with quantitative real-time PCR.

300 onfirmed the results from array studies with quantitative real-time PCR analysis.