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1 rRNA promoters were activated by purified R. sphaeroides
2 rRNA-modifying enzymes participate in ribosome assembly.
3 ned 402 new multigene sequences from the 12S rRNA, 16S rRNA, and tRNA (val) regions of the mitochondr
18 asons examined, as evidenced by abundant 16S rRNA and hgcA transcripts clustering with this family.
20 to our microfluidic nanoarray to amplify 16S rRNA using dPCR and then perform dHRM to identify the tw
22 formed a case-control study by analyzing 16S rRNA microbial profiling, shotgun metagenomics and SCFAs
24 he most widespread ESBL gene family) and 16S rRNA (a proxy for bacterial load) abundance data from 83
25 s question, using metatranscriptomic and 16S rRNA gene profiling techniques to compare the microbiome
27 ased on untargeted mass spectrometry and 16S rRNA gene sequencing, both stress and Test diet altered
28 Using high-resolution metabolomics and 16S rRNA gene sequencing, plasma/urine metabolomes and the f
33 biofilms on MUC2-coated coverslips, and 16S rRNA sequencing showed a unique biofilm profile with sub
36 h-throughput sequencing of the bacterial 16S rRNA gene to assess whether significant vertical stratif
38 udinal metagenomics data, including both 16S rRNA and whole-metagenome shotgun sequencing data, enhan
39 are distinct from the references by both 16S rRNA fractional content and phylogeny, with the former d
40 c clade based on the phylogenies of both 16S rRNA genes and ribosomal proteins, which we propose to n
45 sparse bacterial signal was detected by 16S rRNA sequencing (n = 40 of 50) compared to environmental
50 functions of these species, we combined 16S rRNA sequencing and shotgun metagenomics to characterize
53 ofiles were assessed using datasets from 16S rRNA sequencing, Nanostring miRNA and GC-MS targeted ana
56 cific endoribonuclease that functions in 16S rRNA processing in both alpha- and gamma-proteobacteria.
64 he assay was able to detect amplicons of 16S rRNA and katG mRNA generated from 0.1 pg and 10 pg total
67 gle cells were analysed by sequencing of 16S rRNA genes in the oligotrophic North Pacific Subtropical
69 -pairing interactions with the 3' end of 16S rRNA, mRNA Shine-Dalgarno (SD) sequences positioned upst
71 ng to the metabolic predictions based on 16S rRNA gene sequences, the relative abundance of functiona
75 s race-specific microbiota, we performed 16S rRNA gene-based sequencing of retrospective tumor and ma
76 ive, cross-sectional study, we performed 16S rRNA sequencing on stool swab samples collected from neo
83 ing RNAseq of the citrus host responses, 16S rRNA gene sequencing to characterize citrus-associated m
85 w multigene sequences from the 12S rRNA, 16S rRNA, and tRNA (val) regions of the mitochondrial genome
86 encing of saliva and middle ear samples, 16S rRNA sequencing, molecular modeling, and statistical ana
88 erial taxa were quantified by sequencing 16S rRNA genes in fecal samples collected at 6, 12, 18, and
89 genes were monitored in water and soil, 16S rRNA as an indicator of total bacterial load, intI1 as a
91 study, we sequenced the V6 region of the 16S rRNA gene and used quantitative polymerase chain reactio
95 samples were amplified by targeting the 16S rRNA gene V4 region, and microbial findings were correla
96 , intI1, tet(O), tet(Q), tet(X), and the 16S rRNA gene) decreased significantly in runoff with increa
99 U), and crosslinks specifically with the 16S rRNA, and several mtLSU proteins and assembly factors.
102 thin a farming cohort, were subjected to 16S rRNA amplicon sequencing to characterize bacterial commu
104 by securing efficient methylation of two 16S rRNA residues, and ultimately serves to coordinate subun
106 mollusk-prokaryote interactions, we used 16S rRNA gene amplicons to evaluate how microbial compositio
109 n = 36 pregnant, n = 39 lactating) using 16S rRNA gene amplicon sequencing and assessed whether the r
111 unambiguous species identification using 16S rRNA gene and average nucleotide identity, 2) determinat
113 iations in both health and disease using 16S rRNA gene sequencing of 410 individuals from across the
114 s from 100 Lynch syndrome patients using 16S rRNA gene sequencing of colon biopsies, coupled with met
118 irth cohort, we modeled maturation using 16S rRNA sequence data of the human gut microbiome in infant
119 y characterizing sediment bacteria using 16S rRNA sequences, bacterial community composition of a sed
121 e denitrifying microbial community using 16S rRNA sequencing and quantitative polymerase chain reacti
123 31 non-NAFLD controls are analyzed using 16S rRNA sequencing; an independent Western cohort is used f
127 , oral bacterial community profiling via 16S rRNA sequence analysis remains a valuable technique for
128 d 3 years (n = 140) were quantified with 16S rRNA gene and shotgun metagenomic sequencing (n = 101 si
129 antification of PC intake, together with 16S rRNA gene sequencing of the gut microbiota, and faecal a
131 responding samples were also paired with 16S rRNA sequencing to describe the microbial community and
132 PCR amplification and sequencing of the 16S-rRNA gene, the potential presence of FE producing bacter
134 In this study we explored both 16S and 18S rRNA microbial communities of D. armigerum from both wil
135 ganization, overaccumulation of 5.8S and 18S rRNA precursors, and an imbalanced 40S:60S ribosome subu
136 ted for microbial eukaryote communities (18S rRNA), and (5) developmental stage has an influence on t
138 ke 5 (METTL5) protein catalyzes m(6)A in 18S rRNA at position A(1832) We report that absence of Mettl
139 ficantly decreased m(2) (6,6)A levels in 18S rRNA, indicating a dominant-negative effect of this vari
142 which were applied for visualization of 18S rRNA by fluorescent in situ hybridization in HEK-293T ce
144 I]) and nuclear (small subunit 18S rRNA [18S rRNA]) genes to determine a species-level molecular iden
145 se I [mtCOI]) and nuclear (small subunit 18S rRNA [18S rRNA]) genes to determine a species-level mole
147 idely used barcode, the V9 region of the 18S rRNA gene, to study the effect of environmental conditio
148 ession of SNORD42A with concomitant U116 18S rRNA 2'-O-methylation is essential for leukemia cell gro
149 table during the activation process were 18S rRNA and SDHA mRNA, encouraging their usage as reference
152 ase Center (GAC) RNA domain in bacterial 23S rRNA is directly bound by ribosomal protein L11, and thi
153 confirms known modifications present in 23S rRNA and additionally allows for localization of Mg2+ io
154 rrelated with the presence of alleles of 23S rRNA (A2142G/A2143G) for clarithromycin (kappa coefficie
156 o found to generate the mature 5' end of 23S rRNA, subsequent to a newly identified prior processing
157 ing of several classes of antibiotics to 23S rRNA, resulting in a multidrug-resistant phenotype in ba
158 POET activates hibernating ribosomes via 23S rRNA pseudouridine synthase RluD, which increases riboso
159 nking circularly permutated 5S rRNA with 23S rRNA we generated a bacterial strain devoid of free 5S r
160 th the longest length sequences (16S and 23S rRNAs), as well as a substantial improvement on long-dis
161 ons in the Expansion Segment 7 (ES7L) of 25S rRNA that allowed the formation of mature, translational
163 d compensatory mutations at the 752 and 2609 rRNA positions, we show that integrity of the base pair
166 eps of the two large ribosomal subunit (50S) rRNAs, 23S and 5S pre-rRNAs, are catalyzed by the double
169 choring of the Rpf2 subcomplex containing 5S rRNA, rpL5, rpL11, Rpf2 and Rrs1, which initially docks
173 Our results argue that the autonomy of 5S rRNA is preserved due to its role in ribosome biogenesis
175 bosome engineering to investigate whether 5S rRNA autonomy is critical for ribosome function and cell
178 oduces 12S pre-rRNA, a precursor to the 5.8S rRNA. However, a heterozygous Bccip loss was insufficien
180 port that Fe(2+) promotes degradation of all rRNA species of the yeast ribosome and that it is bound
181 her cNHEJ factors, resides in nucleoli in an rRNA-dependent manner and is co-purified with the small
184 s challenging because ribosomal proteins and rRNA preclude most spectroscopic measurements of protein
185 RNA substrates to fine-tune spliceosomal and rRNA function, accommodating changing requirements for s
186 RNA modification that is present on tRNA and rRNA and has recently been investigated in eukaryotic mR
188 nce identified the genetic background behind rRNA gene mutations causing variable levels of resistanc
189 nctions associated with ribosome biogenesis, rRNA processing, ribosome binding, GTP binding, and hydr
191 xpected gene regulation directly mediated by rRNA and how ribosome evolution drives translation of cr
197 nstrate that Mn(2+) competes with Fe(2+) for rRNA-binding sites and that protection of ribosomes from
198 de that loss of a single enzyme required for rRNA methylation has profound and highly specific effect
199 n assembly of 94-ribosomal proteins and four-rRNAs with an additional protein mass of ~700 kDa on the
200 have been developed to detect circRNAs from rRNA-depleted RNA-seq data based on back-splicing juncti
201 ostic performance of 16S ribosomal RNA gene (rRNA) polymerase chain reaction (PCR)/sequencing of SF a
205 zation highlights the importance of m(6)A in rRNA in stemness, differentiation, development, and dise
207 e specificities and modification patterns in rRNA, and highlight a differential impact of m(4)C methy
208 els of ac(4)C across hundreds of residues in rRNA, tRNA, non-coding RNA and mRNA from hyperthermophil
210 a provide the first structural insights into rRNA maturation in bacteria by revealing how these RNase
211 py approach to detail the protein inventory, rRNA modification state, and structures of 40S ribosomal
212 urified RNA polymerase (RNAP) to investigate rRNA synthesis in the photoheterotrophic alpha-proteobac
213 munity composition by sequencing 16S and ITS rRNA regions and function using community-level physiolo
214 (ESs) consist of multitudes of tentacle-like rRNA structures extending from the core ribosome in euka
215 protection of ribosomes from Fe(2+)-mediated rRNA hydrolysis correlates with the restoration of cell
218 by a nuclear gene, is responsible for 12S mt-rRNA methylation at m(4)C839 both in vivo and in vitro W
221 the functional organization of the multicopy rRNA gene clusters (rDNA) in the nucleolus is less well
225 ly focusing on a line only containing 20% of rRNA gene copies (20rDNA line), we investigated the impa
228 ta255-344 mutation affects proper folding of rRNA helices H68-70 during anchoring of the Rpf2 subcomp
231 total number of tRNA genes, total number of rRNA genes, and codon usage bias in ribosomal protein se
233 ith U3 snoRNA expression led to reduction of rRNA levels and translational capacity, whilst induced e
237 apply our system to analyze the turnover of rRNA during ribophagy induced by oxidative stress or mTO
240 of detection of T. pallidum DNA in blood or rRNA in CSF at the index episode were significantly lowe
241 e-rRNA processing step that produces 12S pre-rRNA, a precursor to the 5.8S rRNA. However, a heterozyg
245 s that despite the variants both driving pre-rRNA processing defects and 80S monosome reduction, the
247 B in atprmt3-2, which accounts for early pre-rRNA processing defects and results in nucleolar stress.
250 BCCIP is a critical factor for mammalian pre-rRNA processing and 60S generation and offer an explanat
252 RNAs (pre-rRNAs) and ribosomal proteins, pre-rRNA processing, and subunit assembly with the aid of nu
253 nine methyltransferase AtPRMT3 regulates pre-rRNA processing; however, the underlying molecular mecha
257 ibosomal subunit (50S) rRNAs, 23S and 5S pre-rRNAs, are catalyzed by the double-strand specific ribon
258 inated expression of pre-ribosomal RNAs (pre-rRNAs) and ribosomal proteins, pre-rRNA processing, and
260 in (Ncl), a nucleolar protein that regulates rRNA synthesis and ribosome biogenesis, interacts with C
266 nd to the ribosome reveal 23S ribosomal RNA (rRNA) nucleotide A1913 positional changes that are depen
267 macrolides interacts with 23S ribosomal RNA (rRNA) nucleotides A752 and U2609, that were proposed to
273 efficient physical removal of ribosomal RNA (rRNA), which otherwise dominates transcriptomic reads.
274 ted two methods for preparing ribosomal RNA (rRNA)-depleted sequencing libraries for RNA-Seq of whole
276 kinds: maxicircles encoding ribosomal RNAs (rRNAs) and proteins and minicircles bearing guide RNAs (
279 s maturation of 18S and 5.8S ribosomal RNAs (rRNAs), and Nop53 is required for maturation of 5.8S and
280 ding RNAs (ncRNAs) including ribosomal RNAs (rRNAs), transfer RNAs (tRNAs), small nuclear RNAs (snRNA
281 tion to study microbiota composition by 16 S rRNA amplicon sequencing and for short-chain fatty acid
282 alpha-helical conformation that induces 28 S rRNA nucleotide rearrangements that suppress the peptidy
284 ed, the P. locustae ribosome retains several rRNA segments absent in other microsporidia, and represe
286 m(4)C methylation in bacterial small subunit rRNA on the ribosome, we found that METTL15 depletion re
287 S. meliloti can reciprocally complement the rRNA processing defect in a DeltaybeY mutant of the othe
288 where they refold and chemically modify the rRNA and prevent early translation before full maturatio
290 ind that addition of hgbRNA depletion to the rRNA-depletion protocol for library preparation from blo
291 n contrast, eIF2alpha-R53 interacts with the rRNA backbone only in the open complex, and the R53E sub
293 ions for adenine at position -7 in the three rRNA promoters strongly increased intrinsic promoter act
295 hat 20 million reads that are not mapping to rRNA/tRNA are required for global detection of translate
296 mplexes, requires the correct folding of two rRNA elements in the subunit head and the proper positio
299 ntisense RNAs are the main substrates, while rRNA, tRNA, and other conserved non-coding RNAs (ncRNAs)
301 hus, distinct interactions of eIF2alpha with rRNA or mRNA stabilize first the open, and then closed,