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1 ic analysis of microtubule pull-downs, using ratiometric (14)N/(15)N labeling, revealed 605 proteins
2 tion in vivo can be imaged in real time with ratiometric activatable cell penetrating peptides (RACPP
9 dentify the regimes in which the widely used ratiometric analysis of signals is substantially subopti
10 thrombin as a model protease, we show that a ratiometric analysis of the emission from the QD, Cy3, a
12 ect hydrogen peroxide in the nucleus through ratiometric analysis of the oxidation of nuclear cystein
15 UV lamp was used as an excitation source and ratiometric analysis using an iPad camera was possible b
16 ended to overcome this technical limitation: ratiometric analysis using monochromatic, evanescent dar
18 The studies showed that 4-MB exhibits a dual ratiometric and calorimetric response toward peroxynitri
20 siological pH of 7.0, have simultaneous self-ratiometric and ratiometric features varying linearly wi
22 fluorescence lifetime spectroscopy with the ratiometric approach and investigate potential non-FRET
26 potential, monitored by the dual wavelength ratiometric approach utilizing the probe di-8-ANEPPS.
30 mutant to increase the ECV pH in the in vivo ratiometric assay suggests that ammonia produced by the
33 we developed an oligonucleotide-based probe, ratiometric bimolecular beacon (RBMB), which generates a
34 uitin assays, they were recovered in vivo by ratiometric bimolecular fluorescence complementation, an
36 ured by X-rhod-1 or mitochondrially-targeted ratiometric biosensor 4mtD3cpv, or ADP-ATP exchange rate
40 xibility in the design of a wide spectrum of ratiometric biosensors and expanded in vivo applications
41 d sensors, ECL resonance energy transfer and ratiometric biosensors and give future perspectives for
43 truct both intermolecular and intramolecular ratiometric biosensors for qualitative imaging of caspas
44 , that predicts the input range of two-state ratiometric biosensors given the experimental error in m
45 a retroreflective marker-based tracking and ratiometric brain imaging system, permitting brain activ
46 a genetically encoded Ca(++) sensor that is ratiometric by virtue of bioluminescence resonance energ
48 ponse of a low-affinity, genetically encoded ratiometric Ca(2)(+) indicator (D4cpv) calibrated agains
56 single-emission biosensors into a series of ratiometric calcium sensors (MatryoshCaMP6s) and ammoniu
58 nt concentration, researchers have developed ratiometric CEST imaging, which investigates contrast ag
61 The derivative L1 detects Zn(2+) by bringing ratiometric change in the fluorescence signals at 390 an
62 GTP binding to these sensors results in a ratiometric change in their fluorescence, thereby provid
65 s(II) and Ag(I) complexes exhibit remarkable ratiometric changes of intensity for phosphorescence ver
66 lities of SWiFi by reporting on mobility and ratiometric characterization of fluorescent nanodiamonds
70 ct quantity versus the donor, we utilize the ratiometric comparison of the two to obtain the stoichio
71 ostic product ions during SRM transitions by ratiometric comparisons with stable isotope internal sta
72 individual protomers in complexes where the ratiometric composition of LHR(B-) to LHR(S-) modulated
74 report the synthesis of a new water-soluble ratiometric Cu(II) dye with a moderate affinity (10(9) M
75 was successfully constructed via fluorescent ratiometric detection and fluorescence lifetime imaging;
76 ng capability in a single vector with robust ratiometric detection by exploiting a network of FRET pa
79 he application of our sensing system for the ratiometric detection of Mg(2+) in target organelles in
80 rescent probes that have been applied to the ratiometric detection of various analytes, including cat
81 in the presence of L-cysteine is slow, but a ratiometric detection process in the therapeutic window
84 e fragmentation strategy that allows for the ratiometric determination of relative H2S and cysteine (
86 the design and fabrication of pH responsive ratiometric dual component sensor systems based on multi
91 e, we have specifically designed a family of ratiometric endosomal pH probes for use in live-cell STE
92 ncy, and discriminate collagen and myosin by ratiometric epi-generated SHG images at 920 nm and 860 n
95 ssion mechanisms, and imaging studies with a ratiometric extracellular zinc sensor probing zinc relea
96 odulate the intensity of such phenomena in a ratiometric fashion, in direct proportion with analyte c
100 mine fluorescence intensities functions as a ratiometric fluorescence chemodosimeter for mercury.
101 igital NAAT platform, termed Droplet Digital Ratiometric Fluorescence Coding (ddRFC), via a padlock p
102 IP-1), a reactivity-based probe that enables ratiometric fluorescence imaging of labile iron pools in
105 H of individual lysosomes using quantitative ratiometric fluorescence microscopy and report an unappr
106 rowing biology, we report a first-generation ratiometric fluorescence resonance energy transfer (FRET
107 al sensing methods currently being explored, ratiometric fluorescence sensing has received particular
109 The o-amino GFPSCs can serve as wavelength-ratiometric fluorescence sensors that selectively recogn
111 a convenient strategy to design an efficient ratiometric fluorescent bioimaging probe for metal ions.
113 e proof of concept that the colorimetric and ratiometric fluorescent cyanide-selective chemodosimeter
116 and dynamics of LH using genetically encoded ratiometric fluorescent heme sensors in the unicellular
117 This feature was utilized for the real-time ratiometric fluorescent imaging of MsrA activity in E. c
118 intracellular copper, we demonstrate with a ratiometric fluorescent indicator, crisp-17, that cytoso
119 H probes for use in live-cell STED nanoscopy.Ratiometric fluorescent pH probes are useful tools to mo
122 signed and synthesized as a colorimetric and ratiometric fluorescent probe that can be utilized to ra
123 e report the design and application of a new ratiometric fluorescent probe, which contains different-
124 er, and then polarized, labeled with JC-1 (a ratiometric fluorescent probe, which indicates changes i
125 ard the design strategy of a new category of ratiometric fluorescent probes facilitating precise and
126 Keima is a pH-sensitive, dual-excitation ratiometric fluorescent protein that also exhibits resis
130 Using this design principle, we generate a ratiometric fluorescent sensor protein for methotrexate
131 acid technology permits the semisynthesis of ratiometric fluorescent sensor proteins with unprecedent
136 vely termed as DC-SIGN/R) using a sensitive, ratiometric Forster resonance energy transfer (FRET) ass
137 as donor showed the highest dynamic range in ratiometric FRET imaging experiments with the G-protein
141 mouse, Arl13b-mCherry-GECO1.2, expressing a ratiometric genetically encoded calcium indicator in all
144 features make it particularly favorable for ratiometric Hg(2+) sensing and bioimaging applications.
149 ral confocal microscopy approach to generate ratiometric images of the plasma membrane surface of Bri
150 large dynamic range of the construct enabled ratiometric imaging (with Rox excitation) of caspase act
154 t insensitive to Cl(-) and pH), we show that ratiometric imaging is strongly affected by the optical
155 We show the use of these BRET systems for ratiometric imaging of both cells in culture and deep-ti
156 Transgenic expression of Salsa6f enables ratiometric imaging of Ca(2+) signals in complex tissue
160 ge of DAF emission in two distinct channels, ratiometric imaging was successfully performed and led t
161 d in Arabidopsis (Arabidopsis thaliana), and ratiometric imaging was used to monitor cytosolic Pi dyn
162 que combination of features: dual-excitation ratiometric imaging, high dynamic range, good signal-to-
174 RACT: The calcium-modulated photoactivatable ratiometric integrator (CaMPARI) is a genetically encode
175 ference for fluorescent indicators to enable ratiometric intensity or dually lifetime referenced meas
176 distinguishable emission bands, allowing for ratiometric intensity-based and time-resolved studies in
177 ere employed as temperature indicators using ratiometric intensity-based fluorescence techniques.
178 ally encoded tag for electron microscopy and ratiometric light microscopy, we go on to show that bulk
179 lipid identification was carried out using a ratiometric lipid standard on a TSQ Quantum Access Max m
180 g, pulse-chase analysis, and high-resolution ratiometric live-cell studies demonstrate that mDia2 and
181 ed for F(-), and the results obtained by our ratiometric luminescent method were consistent with thos
182 ymerization strategy to realize a membranous ratiometric luminescent thermometer based on the emissio
184 and red) that can be used for ultrasensitive ratiometric measurements and fluorescence microscopy.
185 The ability of SWiFi for high-throughput, ratiometric measurements of fast-diffusing species rende
186 oxidation-state specificity and facilitates ratiometric measurements that minimize potential interfe
187 monstrate that SWiFi enables high-throughput ratiometric measurements with several thousands of indiv
188 ator performance such as Kd, wavelength, and ratiometric measurements, the use of NP sensors can achi
191 as off-target binding or uneven delivery, a ratiometric method is employed to quantify the specific
192 Inspired by dual-wavelength fluorescence ratiometric method which could reduce the influence from
193 robust sensor for pH determination using the ratiometric methodology where excitation at a single wav
194 ple targets as well as for implementation of ratiometric methods that can improve accuracy and precis
197 gel transitions, simple changes in color, or ratiometric monitoring of the differences in the fluores
199 ing super-resolution microscope that enables ratiometric multicolor imaging of mammalian cells at 5-1
200 new pathway to design ultrasmall fluorescent ratiometric nanoindicators with tunable wavelengths and
202 cantly increased signal change compared with ratiometric NES-YC3.6 in response to several stimuli.
205 support of multiplexing and error correcting ratiometric or differential measurement approaches.
206 trate that REX-GECO1 can be used as either a ratiometric or intensiometric Ca(2+) indicator in organo
207 iofunctionalised IDE array electrodes gave a ratiometric percentage of sample reading against the bla
208 pH (pHm) and DeltaPsim were monitored using ratiometric pericam and tetramethylrhodamine ethyl ester
209 for the first time proposed an MIs-triggered ratiometric persistent luminescence (R-PersL) sensor arr
212 luorogenic donor functions as a cell surface ratiometric pH indicator, which upon internalization ser
216 NP by fine-tuning of the surface coverage; a ratiometric pH response was then observed owing to stron
221 ystem lumen, we targeted genetically encoded ratiometric pH sensors to the cytosol, the endoplasmic r
224 em dye pair MG-Bis-SA to FAP-EGFR provides a ratiometric pH-sensitive model with dual fluorescence ex
226 998, genetically encoded pH-sensitive sensor ratiometric pHluorin proved to be a valuable tool for ce
227 mitation, we developed a genetically encoded ratiometric pHlys biosensor, pHLARE (pH Lysosomal Activi
228 , we designed APC-1 and APC-2 explicitly for ratiometric photoacoustic imaging by using an aza-BODIPY
230 g the development of the first near-infrared ratiometric photoacoustic probe for in vivo real-time im
232 With respect to single-emission probes, this ratiometric probe has demonstrated to feature excellent
234 is demonstrated utilizing four known optical ratiometric probes and sensors, together with a discussi
237 phoresis, fluorescence guided surgery, or as ratiometric probes for probing biochemical pathways.
239 entified subpopulations of DCIS lesions with ratiometric properties resembling either benign fibroade
240 es on photodissociation of cut s10 from GFP, ratiometric protease sensors were designed from the cons
242 Lipids were identified and subjected to ratiometric quantification using a TSQ quantum Access Ma
243 gnetic resonance (MR) probe that enables the ratiometric quantitation and imaging of pH through chemi
244 f [Ca(2+)], providing the first example of a ratiometric quantitation of Ca(2+) concentration using P
245 Herein, we report a novel dual-affinity ratiometric quenching (DARQ) assay, which combines selec
246 en) in a molecular proximity that results in ratiometric quenching of their fluorescence emission.
251 ts in combination with the recently reported ratiometric resonance synchronous spectroscopic (R2S2, p
253 achieves this with a 2.4% change/ degrees C ratiometric response over physiological temperatures in
255 dual emission exhibits highly sensitive and ratiometric response to solvent polarity, viscosity, tem
258 w, excellent photostability, self-referenced ratiometric response, fast response, and high selectivit
259 orm (low concentration range), it displays a ratiometric response, while a "turn-off" sensing is obse
263 vised sensor film can be used for referenced ratiometric sensing and 2D imaging of pH using a color c
264 uses only a single emitter, which simplifies ratiometric sensing and broadens the applications of the
266 BSA-AuNCs offered a sensitive and reversible ratiometric sensing of a 0.1-pH unit change in the pH ra
268 of a dual emission probe for the fluorescent ratiometric sensing of hydrogen peroxide (H2O2), enzyme
270 ecific repertoire of computations, including ratiometric sensing, balance detection, and imbalance de
272 sion enables the development of the CD-based ratiometric sensor for Fe(III) ions and pyrophosphate ba
275 them ideal candidates for the preparation of ratiometric sensors based on absorption-based indicators
280 d on these results, we further construct two ratiometric sensors using SWCNT/GO nanoscrolls by incorp
282 ports investigation of the dependence of the ratiometric signal on the type of linkage used to conjug
283 lls the sensors showed consistent changes in ratiometric signal upon depletion or restoration of GTP
286 nanosized optical pH probes need to provide ratiometric signals in the optically transparent biologi
287 yield light-up signals of up to 40-fold, or ratiometric signals with ratio changes of 82-fold, on en
291 e combine APEX technology with a SILAC-based ratiometric tagging strategy to substantially reduce unw
292 on describes ultrabright single-nanoparticle ratiometric temperature sensors based on semiconducting
293 4] is an unprecedented case of a luminescent ratiometric thermometer based on a very stable silicate
294 of open cellular regions, we instead use a 'ratiometric' three-state SILAC protocol for high spatial
295 f QD-FRET methods with digital imaging for a ratiometric transduction of nucleic acid hybridization o
296 palette of a digital camera for quantitative ratiometric transduction of nucleic acid hybridization o