ライフサイエンスコーパス: real-time PCR

1 , RNA in situ hybridization and quantitative real time PCR.

2 ping was performed using Taqman chemistry in real time PCR.

3 expression was assessed through quantitative real time PCR.

4 es was measured using multiplex quantitative real time PCR.

5 yzed using histopathological examination and real time-PCR.

6 droplet digital PCR (ddPCR) and quantitative real-time PCR.

7 RNA in situ hybridization, and quantitative real-time PCR.

8 detected by sequencing were confirmed using real-time PCR.

9 evaluated in 69 patients using quantitative real-time PCR.

10 fungal infection and the control corneas by real-time PCR.

11 f 1,202 swab specimens, 105 were positive by real-time PCR.

12 31/42/51 were quantified using type-specific real-time PCR.

13 treptococcus (GAS) assay compared to routine real-time PCR.

14 ); and expression of GLUT4 mRNA in the GM by real-time PCR.

15 rial load was quantitated using quantitative real-time PCR.

16 h measuring the virus amount by quantitative real-time PCR.

17 onding non-cancerous tissues by quantitative Real-Time PCR.

18 by using flow cytometry and in part by using real-time PCR.

19 e expression H4R and RANKL was determined by real-time PCR.

20 , enzymatic assays (ELISA), and quantitative real-time PCR.

21 atients were screened for colonization using real-time PCR.

22 gene expression of key anabolic proteins by real-time PCR.

23 ination in the model foods was determined by real-time PCR.

24 by HDM was quantified by using quantitative real-time PCR.

25 by means of extracellular flux analysis and real-time PCR.

26 receptor 2 (VEGF-R2) in VECs was assessed by real-time PCR.

27 or the detection of Plasmodium species using real-time PCR.

28 rent median strain lifespans by quantitative real-time PCR.

29 anscripts were determined using quantitative real-time PCR.

30 he retinae of these mice was demonstrated by real-time PCR.

31 sured by using a microarray and quantitative real-time PCR.

32 433 (A/G) genotyping was performed by TaqMan Real-Time PCR.

33 he nasopharyngeal swab specimens tested with Real-Time PCR.

34 T expression was measured using quantitative real-time PCR.

35 h immunohistochemistry in liver sections and real-time PCR.

36 Findings were confirmed by traditional real-time PCR.

37 A shedding rates and detection of HIV RNA by real-time PCR.

38 the field, with each then being subjected to real-time PCR.

39 mitochondria and ccf-mtDNA was quantified by real-time PCR.

40 wildlife conservation, we analyzed, through real-time PCR, 152 samples belonging to 14 wild carnivor

41 Of 120 samples tested by individual real-time PCR, 35 were positive for eight different targ

42 the three susceptible loci were measured by real-time PCR after the stimulation by M. leprae antigen

43 cyte telomere length as an internal control (real-time PCR), along with sperm chromatin status (TUNEL

44 TaqMan real-time PCR amplification was used to detect the prese

45 RNA sequencing and quantitative real-time PCR analyses revealed that, although the expre

46 EMSA, ChIP assay and real-time PCR analyses suggest CaWRKY40 binds at the pro

47 Real-time PCR analyses were performed to quantify the le

48 l viability, Western Blotting, Zymogram, and Real-time PCR analyses were performed.

49 oth patients' CSF was repeatedly negative on real-time PCR analysis despite concurrent neurological d

50 evels of microRNAs and mRNAs by quantitative real-time PCR analysis of RNA extracted from plasma, liv

51 Quantitative real-time PCR analysis revealed an intermediate expressi

52 In addition, real-time PCR analysis revealed that chemokines and cyto

53 Real-time PCR analysis revealed that FENDRR was expresse

54 Quantitative real-time PCR analysis was performed to detect and relat

55 RNA sequencing and quantitative real-time PCR analysis were used to assess the transcrip

56 dates for further validation by quantitative real-time PCR analysis.

57 Furthermore, the LC-MS/MS and quantitative real-time-PCR analysis followed by inhibitor and antibod

58 r responses were measured using quantitative real time PCR and multiplex assay.

59 ral culturing, intratypic differentiation by real time-PCR and nucleic acid sequencing of VP1 region

60 onfirmed at the mRNA level with quantitative real-time PCR and at the protein level with ELISA.

61 s was confirmed by viral identification with real-time PCR and by detection of positive anti-orthopox

62 TNF-alpha, and staurosporine), quantitative real-time PCR and clustering analysis, we studied gene-g

63 s, and pluripotency markers were analyzed by real-time PCR and compared with NF human heart.

64 ced microglial ISG responses by quantitative real-time PCR and demonstrated that both were dependent

65 rowing areas of Scotland were assessed using real-time PCR and DNA barcoding techniques.

66 Real-time PCR and ELISA analyses showed that ER stress i

67 Testing for Ebola virus was done by real-time PCR and for malaria by a rapid diagnostic test

68 ne mRNA and protein expression (quantitative real-time PCR and immunofluorescence), microbiota compos

69 ers calretinin and calbindin, as assessed by real-time PCR and immunofluorescence.

70 onlesional, and healthy skin by quantitative real-time PCR and immunohistochemistry, and in blood by

71 regulation of IL-6 compared with control via real-time PCR and immunohistochemistry.

72 alyzed at 24 h or 21 d by using quantitative real-time PCR and immunohistochemistry.

73 Notably, real-time PCR and in situ hybridization (ISH) assays con

74 We use yeast complementation, real-time PCR and proteomics to study putative soybean (

75 n and copy number variation were measured by real-time PCR and Quantitative multiplex fluorescent-PCR

76 were tested for DNA and RNA viruses using a real-time PCR and RT-PCR.

77 modulatory properties was evaluated based on real-time PCR and T-cell proliferation.

78 d suspect screening, along with quantitative real-time PCR and time-resolved amplicon Illumina MiSeq

79 IL-8 and IL-10 mRNA levels were assessed by real-time PCR and Toll like receptor 4 (TLR-4) protein e

80 non-invasive emm1 isolates was quantified by real-time PCR and western blot analyses.

81 P-1alpha, and MIP-2alpha) was measured using real-time PCR and western blotting, respectively.

82 ression of Wnt-related genes, as measured by real-time PCR and western blotting.

83 Real-Time PCR and whole mount in situ hybridization assa

84 Genetic markers were determined by real-time PCR and, with clinical risk factors and Asperg

85 firm the presence of tick-borne pathogens by real-time PCR, and a subset of samples was tested for Bo

86 of approaches, including ChIP, quantitative real-time PCR, and cell migration assays, we primarily f

87 Gene array, real-time PCR, and ELISA analyses showed that treatment

88 Transcriptome analyses, real-time PCR, and immunoblotting showed consistent redu

89 nt growth using plate counting, quantitative real-time PCR, and metagenomics analysis.

90 71) using immunohistochemistry, quantitative real-time PCR, and Singulex in a cross-sectional study.

91 atory indices by using ELISA, histology, and real-time PCR; and type 2 innate lymphoid cells (ILC2s)

92 We used a species-specific real-time PCR approach to detect C. limbatus eDNA in the

93 A normalised real-time PCR approach was also proposed in the range of

94 ee polymorphisms of the CLOCK gene by TaqMan real-time PCR approach, in order to find associations wi

95 We evaluated the performance of a new real-time PCR assay - targeting SP2020 (putative transcr

96 E. histolytica and E. dispar), our tetraplex real-time PCR assay demonstrated levels of sensitivity a

97 infection was assessed with a multiamplicon, real-time PCR assay designed to analyze locations that a

98 nc, Quebec City, QC, Canada) is an automated real-time PCR assay for GAS detection from throat swab s

99 development and evaluation of a novel TaqMan real-time PCR assay for the detection and identification

100 linical performance of a simple, inexpensive real-time PCR assay for the detection of 13 carcinogenic

101 Previously, we developed a real-time PCR assay for the detection of C. auris from s

102 he aim of the present study was to develop a real-time PCR assay for the identification and quantific

103 tilization of an in-house developed C. auris real-time PCR assay for the rapid screening of patient a

104 We developed and validated a TaqMan-based real-time PCR assay on the BD Max platform targeting rib

105 Each emm real-time PCR assay showed high specificity and accurate

106 ompared with those of a laboratory-developed real-time PCR assay targeting repMp1 for detection of M.

107 ompared to that of the Public Health England real-time PCR assay targeting the RdRp gene.

108 e report the development and evaluation of a real-time PCR assay that can be performed directly on cr

109 developed a hydrolysis probe-based tetraplex real-time PCR assay that can simultaneously detect and d

110 A real-time PCR assay was developed for detection of crab,

111 e of C. acutatum in complex olive matrices a real-time PCR assay was developed, using olive drupe and

112 The LOD and LOQ of the real-time PCR assay were 0.1% and 0.4%, respectively.

113 ped and validated the first direct multiplex real-time PCR assay with melt curve analysis for the ide

114 arable with those demonstrated by the duplex real-time PCR assay.

115 enrollment and tested for SARS-CoV-2 using a real-time PCR assay.

116 tick species, showing comparable results to real-time PCR assay.

117 g was performed using a laboratory-developed real-time PCR assay; for the postimplementation group, C

118 In vitro culturing, multiple real-time PCR assays (sodC, ctrA and siaD(B)) and a PorA

119 Using multiple real-time PCR assays across the genome combined with nea

120 cal fungal isolates was used in both PCR and real-time PCR assays followed by electrophoresis or melt

121 oratories and tested by clinically validated real-time PCR assays for Ehrlichia spp., Anaplasma phago

122 For the case of a DNA standard used in real-time PCR assays for human erythropoietin gene, cDNA

123 s were tested at baseline and at 6 months by real-time PCR assays for MG detection in urine samples,

124 f highly sensitive in vitro diagnostic (IVD) real-time PCR assays for respiratory viruses, including

125 performance of the RP panel in comparison to real-time PCR assays for the detection of respiratory pa

126 Global expression analysis and real-time PCR assays revealed that RhERF1 and RhERF4 mod

127 obust, easy-to-perform and interpret PCR and real-time PCR assays to identify C. auris and related sp

128 The emergence of real-time PCR assays utilizing allele-specific molecular

129 ples by in-house nested PCR and quantitative real-time PCR assays, respectively.

130 RP panel with those of laboratory-developed real-time PCR assays, using a variety of previously coll

131 Gold standard is the real-time PCR assays, which can be conducted at highly e

132 pathogens, including HRV, using quantitative real-time PCR assays.

133 sed for real-time polymerase chain reaction (real-time PCR) assessment of markers of bone metabolism

134 s on DNA testing and several conventional or real-time PCR based tests targeting F. circinatum are av

135 This multiplex real-time PCR, based on the dual-labeled probe strategy,

136 The novel, real-time PCR-based GenePOC Carba assay on the microflui

137 We developed a sequential quadriplex real-time PCR-based method for rapid identification of 2

138 We determined that the tetraplex real-time PCR can detect amebic DNA corresponding to as

139 onfirmed using a previously published duplex real-time PCR (capable of detecting E. histolytica and E

140 ulfite sequencing, microarrays, quantitative real-time PCR, colorimetry, Raman spectroscopy to the mo

141 of GABAergic alBST neurons, and quantitative real-time PCR confirmed that GLP1R-KD rats displayed a s

142 y is emerging as diagnostic methods (such as real-time PCR) continue to improve diagnosis.

143 Species-specific eDNA analysis using real-time PCR could therefore represent a cost-effective

144 ge honeybees, while a second method based on real-time PCR coupled to high resolution melting analysi

145 Three defined zones as a function of real-time PCR cycle threshold (Ct) were identified using

146 Currently, the analysis of real-time PCR data is hampered by only considering a sin

147 onal standard curves that extends the use of real-time PCR data obtained by common qPCR instruments.

148 eic acid amplification tests (NAATs) such as real-time PCR demonstrate excellent an limit of detectio

149 Quantitative real-time PCR demonstrated the reduction of AQP5 mRNA ex

150 Quantitative real-time PCR detected an increase in the levels of immu

151 True-positive saliva real-time PCR detection (defined in relation to urine de

152 ribed Spacers region, was used to design the real-time PCR detection assay, resulting in an 490 bp am

153 This combination enabled effective real-time PCR detection of low levels of Salmonella ente

154 by TaqMan array card (TAC), a multipathogen real-time PCR detection platform.

155 d, based on DNA extraction from semolina and real-time PCR determination of T. aestivum in Triticum s

156 B. pseudomallei-infected cases to develop a real-time PCR diagnostic test using two differentially e

157 were assessed by means of Western blotting, real-time PCR, differentiation, and proliferation assays

158 irect gene sequencing, immunohistochemistry, real-time PCR, ELISA, and functional assays in human ker

159 cted from mice and analyzed by histology and real-time PCR; enterocytes were isolated by laser captur

160 of persistent pain, we show by quantitative real-time-PCR, florescence in situ hybridization, Wester

161 and throat swabs were tested using multiplex real-time PCR for 33 respiratory pathogens (FTD((R)) kit

162 Whole blood samples were analysed by real-time PCR for Borrelia burgdorferi s.l., Borrelia mi

163 MC markers were determined by quantitative real-time PCR for chymase and c-kit.

164 DI-TOF MS) for yeast isolate identification, real-time PCR for rapid surveillance sample screening, c

165 CT recipients between 2004 and 2014, in whom real-time PCR for RV was performed in nasopharyngeal asp

166 well as with immunoblotting and quantitative real-time PCR for the expression of stem cell markers.

167 es retinal lesions (both sexes), by means of real-time PCR for the neuronal activity reporter gene zi

168 enomics, we developed an efficient multiplex real-time PCR for the simultaneous detection of all know

169 , lymphocyte immunophenotyping, quantitative real-time PCR from nasopharyngeal swabs, and SARS-CoV-2

170 d the performances of two recently developed real-time PCR HCV RNA assays, cobas HCV for use on the c

171 Single-gene quantitative real-time PCR, immunoblot, and immunofluorescence analys

172 nsporters, RhBG and RhCG, were quantified by real-time PCR, immunoblots, reporter assays, biotin-tagg

173 expression and activity were examined using real-time PCR, immunoblotting and flow cytometry.

174 LncRNA MALAT1 expression (quantitative real-time PCR, immunofluorescence, and RNA sequencing),

175 Here, using cytokine, quantitative real-time PCR, immunoprecipitation, and ChIP assays, alo

176 array of techniques, including quantitative real-time PCR, immunostaining, reporter gene assays, RNA

177 s determined using neutralization assays and real time PCR in BoHV-1 infected Madin-darby bovine kidn

178 We used real time PCR in order to find the relative quantity of

179 M. pneumoniae was detected by real-time PCR in 175 (5.8%) specimens.

180 The fungus was detected using real-time PCR in 26 (8.6%) specimens across the period o

181 the diagnosis of malaria compared to that of real-time PCR in a high-transmission setting.

182 ic enteropathogens in the same samples using real-time PCR in a Taqman array card (TAC) format.

183 perative LD was subsequently confirmed using real-time PCR in an independent validation cohort of 98

184 Mucin 1 expression was evaluated by real-time PCR in human bronchial epithelial cells (HBEC)

185 miR-31 expression was measured by real-time PCR in isolated cholangiocytes.

186 ination of patch-clamp electrophysiology and real-time PCR in MNCs in sham and renovascular hypertens

187 study, we assessed the performance of saliva real-time PCR in newborns undergoing targeted cCMV scree

188 inducible chemokines were evaluated by using real-time PCR in the liver and spleen and by means of EL

189 gated risk factors for KSHV DNA detection by real-time PCR, in blood and viral shedding in saliva, in

190 Real-time PCR is a highly sensitive and powerful technol

191 dergoing targeted screening for cCMV, saliva real-time PCR is highly sensitive yet has a low positive

192 commercially available kit and detected via real-time PCR IS6110 amplification.

193 Real-time PCR measurements of SK channel subunits mRNA i

194 ert Carba-R assay is a qualitative multiplex real-time PCR method that qualitatively detects and diff

195 Using real-time PCR method, we studied the expression of GATA

196 Event- and construct-specific real-time PCR methods for detection of the GM strain and

197 ccius capensis) and using a species-specific real-time PCR MMER_VIC system was achieved using a relat

198 position, by in situ RT-PCR and quantitative real-time PCR of laser microdissected islets for gene ex

199 Out of 21 clinical samples tested by real-time PCR, only 1 was positive and 13 were negative

200 t the use of protocols using conventional or real-time PCR outside their initial development and vali

201 g of soybean DNA (8.6 copies), with adequate real-time PCR performance parameters, regardless of the

202 tive, and broad-range allele-specific TaqMan real-time PCR platform consisting of 7 simultaneous assa

203 to probability zones based on the commercial real-time PCR platform.

204 dies (sensitivity) in a subset of SARS-CoV-2 real-time PCR-positive cases.

205 g; DNA methylation by restriction digest and real-time PCR(qAMP); and expression of GLUT4 mRNA in the

206 PARalpha and CYP4A mRNA was quantified using real-time PCR (qPCR) and CYP4A protein expression, using

207 analysis, correlation and network analysis, real-time PCR (qPCR) and immunohistochemistry.

208 to identify reference genes for quantitative real-time PCR (qPCR) and validate RNAi responses in SPB

209 Quantitative real-time PCR (qPCR) and Western blot analyses confirmed

210 stic dogs for these causative agents include real-time PCR (qPCR) assays in both singleplex and multi

211 and identification of all bacterial species; real-time PCR (qPCR) assays targeting the femA or lytA g

212 loped both single and multiplex quantitative real-time PCR (qPCR) assays that can accurately detect a

213 Quantitative real-time PCR (qPCR) is well suited to the detection of

214 well as targeted PCR/analytically validated real-time PCR (qPCR) with bidirectional sequencing.

215 elected for validation by using quantitative real-time PCR (qPCR), all of which were successfully con

216 tate-of-the-art applications of quantitative real-time PCR (qPCR), next-generation sequencing (NGS) a

217 in situ hybridization (ISH) and quantitative real-time PCR (qPCR), we assess the mRNA distribution an

218 fferent stress conditions using quantitative real-time PCR (qPCR).

219 al density, flow cytometry, and quantitative real-time PCR (qPCR).

220 ntional laboratory-based instruments such as real-time PCR (qPCR).

221 reverse transcriptase PCR, and quantitative real-time PCR (qPCR).

222 As including northern blotting, quantitative real time PCR (qRT-PCR) and microarray technology beside

223 s that code identified protein, quantitative real time PCR (qRT-PCR) was performed.

224 These miRs were validated by quantitative real time-PCR (qRT-PCR) in 43 patients with different tu

225 Quantitative real-time PCR (qRT-PCR) analysis of selected genes also

226 Two quantitative assays, quantitative real-time PCR (qRT-PCR) and droplet digital PCR (ddPCR),

227 Quantitative real-time PCR (qRT-PCR) experiment results of lncRNAs, p

228 sing mature miRNA profiling and quantitative real-time PCR (qRT-PCR) in the orbitofrontal cortex (OFC

229 sion of 41 unigenes measured by quantitative real-time PCR (qRT-PCR) showed consistent results with t

230 tion of candidate markers using quantitative real-time PCR (qRT-PCR) showed that two markers (arsR [N

231 Using quantitative real-time PCR (qRT-PCR) we have measured the gene expres

232 Using quantitative real-time PCR (qRT-PCR), it showed that the DRELFA is ve

233 dontally healthy controls using quantitative real-time PCR (qRT-PCR).

234 ion process was validated using quantitative real-time PCR (qRT-PCR).

235 The development and application of multiplex real-time PCR reactions to the TAC microfluidic platform

236 screening method was developed, based on two real-time PCR reactions, targeting i) six major GM-marke

237 ty is not achieved by conventional PCR while real-time PCR requires expensive and sophisticated instr

238 CR platform with its MGB Alert M. pneumoniae real-time PCR research use only reagents (ELITechGroup,

239 SARS-CoV-2 positives (defined as a positive real-time PCR result; prevalence 7.6%).

240 Saliva real-time PCR results were prospectively correlated with

241 Microarray analysis in conjunction with real-time PCR revealed a higher mRNA expression of Bruto

242 peripheral blood mononuclear cells (PBMC) by real-time PCR revealed levels similar to those in monkey

243 Further evaluation using quantitative real-time PCR revealed that differentially expressed pat

244 Real time-PCR reveals significant reductions in expressi

245 ues such as polymerase chain reaction (PCR), real time PCR (RT-PCR) and dot blot hybridization have a

246 Selected genes were further validated using real time PCR (RT-PCR).

247 ere selected to be evaluated by quantitative real time PCR (RT-qPCR), and also functional in vitro an

248 s culture methods to determine toxigenicity, real-time PCR (RT-PCR) provides a faster method to detec

249 at we would have performed more than 150,000 real-time PCR (RT-PCR) tests, with many more to come.

250 nal gas chromatography-mass spectrometry and real-time PCR (RT-PCR), respectively.

251 slide agglutination serogrouping (SASG) and real-time PCR (RT-PCR).

252 eat mixtures using both conventional PCR and real-time PCR (RT-PCR).

253 etection (reverse transcription-quantitative real-time PCR [RT-qPCR]) and genotyping (endpoint RT-PCR

254 High-throughput sequencing and quantitative real-time PCR showed a significant compositional shift,

255 d amplification on QuantStudio 6 or ABI 7500 real-time PCR system [abbreviated CDC COV]) to detect se

256 PCR system [Stratagene-Agilent] and the 7500 real-time PCR system [ABI Life Technologies]).

257 posing a novel specific and highly sensitive real-time PCR system for the detection/quantification of

258 system and the Applied Biosystems (ABI) 7500 real-time PCR system.

259 In this work, two real-time PCR systems based on the EvaGreen dye and a Ta

260 nsitive detection at sites not equipped with real-time PCR systems required for qPCR diagnostics.

261 his work describes the development of a new, real-time PCR TaqMan assay for the detection of ling (Mo

262 Real-time PCR targeting lytA (the major autolysin gene)

263 difficile status was assessed by GDH EIA and real-time PCR targeting the toxin A (tcdA) and B (tcdB)

264 he method is based on the widely used TaqMan real-time PCR technology and combines allele-specific PC

265 nducted to evaluate the NeuMoDx GBS assay, a real-time PCR test performed for vaginal/rectal swab spe

266 Lesional swabs were collected for real-time PCR testing for T. pallidum subsp pertenue and

267 Four commercial real-time PCR tests, toxin antigen detection by enzyme i

268 o allows extension to integrate with in situ real-time PCR thermal cycling since the sample slide is

269 t device are in accordance with the standard real-time PCR, thus supporting the accuracy of the metho

270 rebral artery occlusion (tMCAO) and measured real-time PCR to detect miR-34a levels.

271 rument is a new molecular platform that uses real-time PCR to detect nucleic acids in up to 8 specime

272 next-generation sequencing and quantitative real-time PCR to determine the impact of dry cow therapy

273 rthermore, we used cycle threshold values of real-time PCR to guide the choice of protocols for SNP a

274 ere validated empirically using quantitative real-time PCR to measure gene expression.

275 emistry, immunoblotting, flow cytometry, and real-time PCR to quantify gene expression.

276 le cycle fluorescence detection, rather than real-time PCR to reduce significantly the time taken to

277 asmonic photothermal method for quantitative real-time PCR, using gold bipyramids and light to achiev

278 s biology data analysis, in combination with real-time PCR validation indicates direct functional inv

279 e University of Alabama at Birmingham, where real-time PCR was performed for detection of 23S rRNA mu

280 Real-time PCR was used as a screening tool with great ac

281 Real-time PCR was used for quantitative analysis of Aggr

282 Real-time PCR was used to detect the expression of HIF-1

283 Real-time PCR was used to study expression of antiviral

284 two-step algorithm compared to results with real-time PCR were 95.5% (95% CI, 90.5 to 98.0%) and 91.

285 ive and negative predictive values of saliva real-time PCR were 98.3% (95% confidence interval, 90.8%

286 cificity of the RDT compared to results with real-time PCR were 99.4% (95% confidence interval [CI],

287 n additional 7 samples that were negative by real-time PCR were positive with T2MR.

288 Western blotting and Real-time PCR were used to assess the expressions of BDN

289 Microarray analysis and real-time PCR were used to examine transcriptional diffe

290 differentiation, flow cytometry, ELISA, and real-time PCR were used to investigate the effects of Bl

291 Real time PCR, western blot and knock down assays demons

292 sessed using luciferase assays, quantitative real-time PCR, western blots, scratch assays, CCK-8 assa

293 h-glucose-treated HK-2 cells was analyzed by real-time PCR, western blotting, and immunohistochemical

294 MUC4 and MUC4beta was evaluated by means of real-time PCR, Western blotting, and immunohistochemistr

295 d cell sorting, RNA sequencing, quantitative real-time PCR, Western blotting, small interfering RNA i

296 An exception is real-time PCR, which can provide quantitative results us

297 amily member D) was lower in NF1-iN cells by real-time PCR with 12 sex-mixed samples.

298 um avium subspecies paratuberculosis through real-time PCR with a limit-of-detection of 20 fg, equiva

299 Species-specific PCR and real-time PCR with EvaGreen dye targeting the ITS region

300 /ml) was detected in FCDI cell lysates using real-time PCR with greater consistency than with prepara